Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.95.0_3-s36-3
Kurotaki Daisuke
We have been studying the mechanism of myeloid cell development from the viewpoint of chromatin regulation by transcription factors. The transcription factor IRF8 is essential for the development of monocytes and dendritic cells (DCs), whereas it inhibits neutrophilic differentiation. We have demonstrated that IRF8 establishes enhancer landscapes in myeloid progenitors to epigenetically prime these cells to differentiate into monocytes or DCs. Recently, we focus on high-order chromatin structure during DC development. Our Hi-C data revealed that DC-specific active compartments are gradually established throughout the course of differentiation, while most of the topologically associating domains (TADs) specific for the DC lineage are formed with slower kinetics. We also found that the activation of DC-specific enhancers proceeds the compartment switch and subsequent gene expression. In addition, our data suggest that IRF8 is required for many of the DC-specific changes from compartment B to A. Collectively, myeloid cell gene expression patterns are epigenetically regulated by key transcription factors such as IRF8 via enhancer establishment and chromatin structure reorganization. Symposium 36
{"title":"Epigenetic regulation of myeloid cell differentiation","authors":"Kurotaki Daisuke","doi":"10.1254/jpssuppl.95.0_3-s36-3","DOIUrl":"https://doi.org/10.1254/jpssuppl.95.0_3-s36-3","url":null,"abstract":"We have been studying the mechanism of myeloid cell development from the viewpoint of chromatin regulation by transcription factors. The transcription factor IRF8 is essential for the development of monocytes and dendritic cells (DCs), whereas it inhibits neutrophilic differentiation. We have demonstrated that IRF8 establishes enhancer landscapes in myeloid progenitors to epigenetically prime these cells to differentiate into monocytes or DCs. Recently, we focus on high-order chromatin structure during DC development. Our Hi-C data revealed that DC-specific active compartments are gradually established throughout the course of differentiation, while most of the topologically associating domains (TADs) specific for the DC lineage are formed with slower kinetics. We also found that the activation of DC-specific enhancers proceeds the compartment switch and subsequent gene expression. In addition, our data suggest that IRF8 is required for many of the DC-specific changes from compartment B to A. Collectively, myeloid cell gene expression patterns are epigenetically regulated by key transcription factors such as IRF8 via enhancer establishment and chromatin structure reorganization. Symposium 36","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75375543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.95.0_1-sl03
S. Okabe
The development of neuronal circuits in vivo depends on precise regulation of synapse formation, elimination, and remodeling. In vivo two-photon imaging confirmed the presence of two phases of synapse dynamics in the postnatal mouse cortex. In the first phase (until postnatal 20 days), synapse turnover is maintained to be high, while in the second phase (after three weeks postnatal), synapse dynamics are highly suppressed, leading to the maturation of the cortical neural network. The transition in synapse dynamics may underlie the pathophysiology of neurodevelopmental disorders and psychiatric diseases, but their precise mechanisms have not yet been clarified. Our laboratory has focused on (1) the diverse mechanisms in neural circuits and synapse formation, (2) the structure-function relationship in dynamic synaptic changes, and (3) the relationship between brain diseases and synaptic dysfunction. In particular, we have recently developed new tools for studying neural circuits, such as quantitative methods for analyzing the nano-structure of spine synapses and methods for measuring the molecular dynamics inside spines. Furthermore, we are applying these tools to the study of brain pathology. In this talk, I will introduce these researches and discuss the validity and prospects of understanding the pathogenesis of psychiatric disorders as synaptic dysfunction.
{"title":"Synapse formation and remodeling visualized in physiological and pathological states","authors":"S. Okabe","doi":"10.1254/jpssuppl.95.0_1-sl03","DOIUrl":"https://doi.org/10.1254/jpssuppl.95.0_1-sl03","url":null,"abstract":"The development of neuronal circuits in vivo depends on precise regulation of synapse formation, elimination, and remodeling. In vivo two-photon imaging confirmed the presence of two phases of synapse dynamics in the postnatal mouse cortex. In the first phase (until postnatal 20 days), synapse turnover is maintained to be high, while in the second phase (after three weeks postnatal), synapse dynamics are highly suppressed, leading to the maturation of the cortical neural network. The transition in synapse dynamics may underlie the pathophysiology of neurodevelopmental disorders and psychiatric diseases, but their precise mechanisms have not yet been clarified. Our laboratory has focused on (1) the diverse mechanisms in neural circuits and synapse formation, (2) the structure-function relationship in dynamic synaptic changes, and (3) the relationship between brain diseases and synaptic dysfunction. In particular, we have recently developed new tools for studying neural circuits, such as quantitative methods for analyzing the nano-structure of spine synapses and methods for measuring the molecular dynamics inside spines. Furthermore, we are applying these tools to the study of brain pathology. In this talk, I will introduce these researches and discuss the validity and prospects of understanding the pathogenesis of psychiatric disorders as synaptic dysfunction.","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75518180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.95.0_1-ss-61
S. Jonan, Ayana Fujiwara, K. Iwata, S. Kato, K. Amagase
{"title":"Alleviative effect of glutamate on 5-fluorouracil-induced intestinal mucositis in mice","authors":"S. Jonan, Ayana Fujiwara, K. Iwata, S. Kato, K. Amagase","doi":"10.1254/jpssuppl.95.0_1-ss-61","DOIUrl":"https://doi.org/10.1254/jpssuppl.95.0_1-ss-61","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75554136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.95.0_2-s25-1
Okada Takaharu
{"title":"Induction of itch and modulation of inflammation by IL-31-responsive sensory neurons","authors":"Okada Takaharu","doi":"10.1254/jpssuppl.95.0_2-s25-1","DOIUrl":"https://doi.org/10.1254/jpssuppl.95.0_2-s25-1","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75709903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.95.0_2-yia-44
R. Hosoda, A. Kuno, Ryuta Nakashima, S. Asakura, N. Iwahara, Iyori Nojima, Risa Kunimoto, Y. Horio
{"title":"Resveratrol attenuates age-related muscle atrophy and motor dysfunction by promoting autophagy in mice","authors":"R. Hosoda, A. Kuno, Ryuta Nakashima, S. Asakura, N. Iwahara, Iyori Nojima, Risa Kunimoto, Y. Horio","doi":"10.1254/jpssuppl.95.0_2-yia-44","DOIUrl":"https://doi.org/10.1254/jpssuppl.95.0_2-yia-44","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73949551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.96.0_4-b-s38-4
Kaneko Naoko
{"title":"Interaction between new neurons and astrocytes in the post-stroke brain","authors":"Kaneko Naoko","doi":"10.1254/jpssuppl.96.0_4-b-s38-4","DOIUrl":"https://doi.org/10.1254/jpssuppl.96.0_4-b-s38-4","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72611330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.96.0_1-b-p-056
Ayaka Kishi, Yuu Tachibana, Yui Murase, Koga Tokuyama, M. Saito, Hiroyuki Yasuda, Kenjiro Matsumoto, S. Kato
{"title":"Orphan G protein-coupled receptor GPR35 contributes to the pathogenesis of dextran sulfate sodium-induced colitis in mice.","authors":"Ayaka Kishi, Yuu Tachibana, Yui Murase, Koga Tokuyama, M. Saito, Hiroyuki Yasuda, Kenjiro Matsumoto, S. Kato","doi":"10.1254/jpssuppl.96.0_1-b-p-056","DOIUrl":"https://doi.org/10.1254/jpssuppl.96.0_1-b-p-056","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"105 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78629262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.96.0_1-b-w05-2
Yoshioka Yusuke
{"title":"Role of extracellular vesicles in disease: a novel tool for understanding disease mechanism?","authors":"Yoshioka Yusuke","doi":"10.1254/jpssuppl.96.0_1-b-w05-2","DOIUrl":"https://doi.org/10.1254/jpssuppl.96.0_1-b-w05-2","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78729311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.95.0_1-yia-15
Tokunaga Nozomi, Yoki Nakamura, K. Nakashima, N. Morioka
{"title":"Downregulation of astrocytic connexin43 potentiates the antidepressant-induced brain-derived neurotrophic factor expression","authors":"Tokunaga Nozomi, Yoki Nakamura, K. Nakashima, N. Morioka","doi":"10.1254/jpssuppl.95.0_1-yia-15","DOIUrl":"https://doi.org/10.1254/jpssuppl.95.0_1-yia-15","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"116 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76001583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.96.0_2-b-s17-2
Takashi Uehara
{"title":"Development of Molecule-Specific Oxidative Modification Inhibitor","authors":"Takashi Uehara","doi":"10.1254/jpssuppl.96.0_2-b-s17-2","DOIUrl":"https://doi.org/10.1254/jpssuppl.96.0_2-b-s17-2","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72659880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：