Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.96.0_yia02-6
D. Sato, Nozomi Imaizumi, M. Kusunoki, L. Miyamoto
{"title":"Involvement of Pgc-1α in the skeletal muscle on glucose uptake via the peripheral sympathetic nervous system in rats","authors":"D. Sato, Nozomi Imaizumi, M. Kusunoki, L. Miyamoto","doi":"10.1254/jpssuppl.96.0_yia02-6","DOIUrl":"https://doi.org/10.1254/jpssuppl.96.0_yia02-6","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84098465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.95.0_1-p-084
Yuki Kaguchi, Yuto Hoshino, Yuichi Fukuyama, Takuya Yamaguchi, J. Yamazaki
{"title":"Role of TRPV1 in hyaluronan-induced apoptosis avoidance in MCF-7 cells","authors":"Yuki Kaguchi, Yuto Hoshino, Yuichi Fukuyama, Takuya Yamaguchi, J. Yamazaki","doi":"10.1254/jpssuppl.95.0_1-p-084","DOIUrl":"https://doi.org/10.1254/jpssuppl.95.0_1-p-084","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84145193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.96.0_yia05-3
Haruka Furuta, Mari Yamada, T. Nagashima, Shuichi Matsuda, K. Nagayasu, H. Shirakawa, S. Kaneko
{"title":"Identification of the therapeutic target for tendinopathy through the combination of real world data analysis and pharmacological experiments","authors":"Haruka Furuta, Mari Yamada, T. Nagashima, Shuichi Matsuda, K. Nagayasu, H. Shirakawa, S. Kaneko","doi":"10.1254/jpssuppl.96.0_yia05-3","DOIUrl":"https://doi.org/10.1254/jpssuppl.96.0_yia05-3","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84153420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.95.0_3-sl11
R. Nishinakamura
Recapitulating the three-dimensional organ structure in vitro is a major challenge for developmental biology and regenerative medicine. The kidney develops by the reciprocal interactions between the nephron progenitor and ureteric bud, and the origins of these two types of precursors are spatially distinct. Based on these developmental findings, we previously established the induction protocols of the nephron progenitors from pluripotent stem cells (PSCs). Induced nephron progenitors robustly formed nephrons: glomeruli and renal tubules. By generating iPS cellderived nephron organoids from a patient with the congenital nephrotic syndrome, we reproduced the glomerular abnormalities that represent the initial phase of this disease. We also established the protocols to induce the ureteric bud from mouse and human PSCs. Mouse organoids reassembled from the differentially induced ureteric bud and nephron progenitors developed the inherent architectures of the embryonic kidney. Humans ureteric bud organoids were applied to autosomal dominant polycystic kidney disease to successfully reproduce cyst formation in vitro. Thus, kidney organoids will serve as useful basis to analyze human kidney development and disease.
{"title":"Organoid-based analysis of human kidney development and disease","authors":"R. Nishinakamura","doi":"10.1254/jpssuppl.95.0_3-sl11","DOIUrl":"https://doi.org/10.1254/jpssuppl.95.0_3-sl11","url":null,"abstract":"Recapitulating the three-dimensional organ structure in vitro is a major challenge for developmental biology and regenerative medicine. The kidney develops by the reciprocal interactions between the nephron progenitor and ureteric bud, and the origins of these two types of precursors are spatially distinct. Based on these developmental findings, we previously established the induction protocols of the nephron progenitors from pluripotent stem cells (PSCs). Induced nephron progenitors robustly formed nephrons: glomeruli and renal tubules. By generating iPS cellderived nephron organoids from a patient with the congenital nephrotic syndrome, we reproduced the glomerular abnormalities that represent the initial phase of this disease. We also established the protocols to induce the ureteric bud from mouse and human PSCs. Mouse organoids reassembled from the differentially induced ureteric bud and nephron progenitors developed the inherent architectures of the embryonic kidney. Humans ureteric bud organoids were applied to autosomal dominant polycystic kidney disease to successfully reproduce cyst formation in vitro. Thus, kidney organoids will serve as useful basis to analyze human kidney development and disease.","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84166149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.95.0_1-o-030
Momoko Goto, Toya Okawa, Daiki Shirane, Hiroki Tanaka, H. Akita, A. Hisaka, Hiromi Sato
Metastatic brain tumors emit various components, including cyclic 2'3'-GMP-AMP (cGAMP) to surrounding astrocytes, which may affect the tumor microenvironments. To identify the cGAMP targets, we analyzed transcriptome in cGAMP-incorporated astrocytes. cGAMP is encapsulated in lipid nanoparticles (ssPalm), then introduced into primary cultured astrocytes. After extracting the RNA, the fold change (FC) by cGAMP was investigated by microarray. Among the genes with high intrinsic expression levels, we searched for interacting factors with large FC in STRING's protein-protein interaction database. As an indicator of astrocyte responsiveness, changes in intracellular calcium concentration were observed by confocal time-lapse imaging.
{"title":"Effect of tumor releasing factor, cGAMP on gene expression and calcium response in astrocytes","authors":"Momoko Goto, Toya Okawa, Daiki Shirane, Hiroki Tanaka, H. Akita, A. Hisaka, Hiromi Sato","doi":"10.1254/jpssuppl.95.0_1-o-030","DOIUrl":"https://doi.org/10.1254/jpssuppl.95.0_1-o-030","url":null,"abstract":"Metastatic brain tumors emit various components, including cyclic 2'3'-GMP-AMP (cGAMP) to surrounding astrocytes, which may affect the tumor microenvironments. To identify the cGAMP targets, we analyzed transcriptome in cGAMP-incorporated astrocytes. cGAMP is encapsulated in lipid nanoparticles (ssPalm), then introduced into primary cultured astrocytes. After extracting the RNA, the fold change (FC) by cGAMP was investigated by microarray. Among the genes with high intrinsic expression levels, we searched for interacting factors with large FC in STRING's protein-protein interaction database. As an indicator of astrocyte responsiveness, changes in intracellular calcium concentration were observed by confocal time-lapse imaging.","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84189753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.95.0_1-p-014
Seki Haruka, F. Takata, Yuki Egashira, T. Iwao, Miki Yokoya, Miho Yasunaga, Naotaka Aridome, Yuko Fukunaga, S. Dohgu
{"title":"PDGF-BB-stimulated brain pericytes release the mediators related to blood-brain barrier dysfunction.","authors":"Seki Haruka, F. Takata, Yuki Egashira, T. Iwao, Miki Yokoya, Miho Yasunaga, Naotaka Aridome, Yuko Fukunaga, S. Dohgu","doi":"10.1254/jpssuppl.95.0_1-p-014","DOIUrl":"https://doi.org/10.1254/jpssuppl.95.0_1-p-014","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"10 4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78346805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.96.0_2-b-p-177
Noriaki Samukawa, Takehiro Yamaguchi, K. Samukawa, Kentaro Tokudome, T. Homma, S. Matsunaga, S. Tomita
{"title":"Antimicrobial activities of ginseng saponins isolated from Red Ginseng to non-tuberculous mycobacteria","authors":"Noriaki Samukawa, Takehiro Yamaguchi, K. Samukawa, Kentaro Tokudome, T. Homma, S. Matsunaga, S. Tomita","doi":"10.1254/jpssuppl.96.0_2-b-p-177","DOIUrl":"https://doi.org/10.1254/jpssuppl.96.0_2-b-p-177","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77766612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.95.0_3-p-260
Riku Sato, Megumi Kanai, Miho Wakamatsu, Kotomi Kanagaki, Yoshimitsu Goto, Takeshi Yamaguchi, N. Iigima, K. Hamada, Toshiyuki Hamada
{"title":"The analysis of Period1 gene expression in vivo and in vitro using a micro PMT system at the early stage in diabets","authors":"Riku Sato, Megumi Kanai, Miho Wakamatsu, Kotomi Kanagaki, Yoshimitsu Goto, Takeshi Yamaguchi, N. Iigima, K. Hamada, Toshiyuki Hamada","doi":"10.1254/jpssuppl.95.0_3-p-260","DOIUrl":"https://doi.org/10.1254/jpssuppl.95.0_3-p-260","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"84 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78070636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.95.0_1-p-001
Ishikawa Hiroki, S. Namiki, D. Asanuma, K. Hirose
Synaptic transmission is regulated by nanoscale assembly of synaptic molecules. Stimulated emission depletion (STED) microscopy is a modality of superresolution microscopy that allows visualizing such nanoscale molecular distribution. However, photobleaching severely limits time-series imaging by STED microscopy in living neurons. In this study, we aimed to develop a fluorescence labeling method that circumvents the limitation due to photo-bleaching in STED microscopy. Our method is based on a fusion protein tag that reversibly binds a small organic dye and turns on its fluorescence emission. We reasoned that this reversibility in binding enables everlasting imaging because the bleached dye should be continuously displaced by a fresh dye. Accordingly, we prepared expression constructs of the protein tag fused with synaptic molecules, RimBP2, CAST, and Rim1a. When expressed in cultured hippocampal neurons, the synaptic localization of these molecules was visualized under a standard fluorescence microscope. We successfully performed STED imaging of RimBP2 and CAST with the spatial resolution of less than 100 nm at 0.2 Hz for 10 min. Thus, our fluorescence labeling method enables live STED microscopy in neurons which will be useful to unveil the dynamic feature of nanoscale molecular distribution underlying synaptic function. Poster Session
{"title":"Development of a fluorescent labeling method for the live-cell superresolution imaging of synaptic molecules.","authors":"Ishikawa Hiroki, S. Namiki, D. Asanuma, K. Hirose","doi":"10.1254/jpssuppl.95.0_1-p-001","DOIUrl":"https://doi.org/10.1254/jpssuppl.95.0_1-p-001","url":null,"abstract":"Synaptic transmission is regulated by nanoscale assembly of synaptic molecules. Stimulated emission depletion (STED) microscopy is a modality of superresolution microscopy that allows visualizing such nanoscale molecular distribution. However, photobleaching severely limits time-series imaging by STED microscopy in living neurons. In this study, we aimed to develop a fluorescence labeling method that circumvents the limitation due to photo-bleaching in STED microscopy. Our method is based on a fusion protein tag that reversibly binds a small organic dye and turns on its fluorescence emission. We reasoned that this reversibility in binding enables everlasting imaging because the bleached dye should be continuously displaced by a fresh dye. Accordingly, we prepared expression constructs of the protein tag fused with synaptic molecules, RimBP2, CAST, and Rim1a. When expressed in cultured hippocampal neurons, the synaptic localization of these molecules was visualized under a standard fluorescence microscope. We successfully performed STED imaging of RimBP2 and CAST with the spatial resolution of less than 100 nm at 0.2 Hz for 10 min. Thus, our fluorescence labeling method enables live STED microscopy in neurons which will be useful to unveil the dynamic feature of nanoscale molecular distribution underlying synaptic function. Poster Session","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79935412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1254/jpssuppl.96.0_1-b-p-068
Natsumi Mizuno, S. Shiga, Y. Yanagawa
{"title":"CDK8/19 inhibitors induce M2-like macrophage polarization.","authors":"Natsumi Mizuno, S. Shiga, Y. Yanagawa","doi":"10.1254/jpssuppl.96.0_1-b-p-068","DOIUrl":"https://doi.org/10.1254/jpssuppl.96.0_1-b-p-068","url":null,"abstract":"","PeriodicalId":20464,"journal":{"name":"Proceedings for Annual Meeting of The Japanese Pharmacological Society","volume":"258 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76765180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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