Pub Date : 2018-08-06DOI: 10.23751/PN.V20I1-S.5993
M. Shidfar, S. Keskin, E. Khah, Petropoulos Spiridon, F. A. Özdemir, Ibrahim Samet Gokcen
Fifteen Random Amplified Polymorphic DNA (RAPD) markers were used to measure genetic diversity and genetic relationships between five endemic genera of Mediterranean basin (Greece). Three species of Cichorium spinosum were collected; two from island Crete (Greece) and one from island Kythnos, and other two species of Taraxacom sp. (Asteraceae); are from Orhomenos and Athens. Two hundred-forty amplified products and 163 RAPD bands were scored with an average of 67.91% of them revealing polymorphism across accessions. In this research OPD-05 primer with 24 bands showed the highest number of bands, while the OPM-18 and OPB-16, both of them with 8 bands showed the least number of bands. Also OPV-06 primer with 18 polimorphic bands showed the highest number of bands. The least number of polymorphic bandswere found in OPX-18. UBC-292, OPAN-01, OPB-16, OPM-18, OPD-05 primers. Subsequently, OPD-05 primer with 29.16% showed the least percentage of polymorphism degree, whereas OPM-18 and OPB-16 primers with 87.50% showed the highest percentage of polymorphism. UPGMA clustering based on data from polymorphic RAPD bands revealed two distinct group which joined to form one major cluster at 32% level of similarity. Also Cichorium spinosum, Crete and Cichorium spinosum, Kythnos, varieties with 100% similarity are synonyms. The similarity indices of the RAPD dendrogram ranged between 30% and 100% averagely high enough to suggest useful variability for genetic diversity and plant breeding.
{"title":"RAPD markers reveal genetic variation between Cichorium spinosum L. and Taraxacum sp.; a substantial medicinal plants of Greece","authors":"M. Shidfar, S. Keskin, E. Khah, Petropoulos Spiridon, F. A. Özdemir, Ibrahim Samet Gokcen","doi":"10.23751/PN.V20I1-S.5993","DOIUrl":"https://doi.org/10.23751/PN.V20I1-S.5993","url":null,"abstract":"Fifteen Random Amplified Polymorphic DNA (RAPD) markers were used to measure genetic diversity and genetic relationships between five endemic genera of Mediterranean basin (Greece). Three species of Cichorium spinosum were collected; two from island Crete (Greece) and one from island Kythnos, and other two species of Taraxacom sp. (Asteraceae); are from Orhomenos and Athens. Two hundred-forty amplified products and 163 RAPD bands were scored with an average of 67.91% of them revealing polymorphism across accessions. In this research OPD-05 primer with 24 bands showed the highest number of bands, while the OPM-18 and OPB-16, both of them with 8 bands showed the least number of bands. Also OPV-06 primer with 18 polimorphic bands showed the highest number of bands. The least number of polymorphic bandswere found in OPX-18. UBC-292, OPAN-01, OPB-16, OPM-18, OPD-05 primers. Subsequently, OPD-05 primer with 29.16% showed the least percentage of polymorphism degree, whereas OPM-18 and OPB-16 primers with 87.50% showed the highest percentage of polymorphism. UPGMA clustering based on data from polymorphic RAPD bands revealed two distinct group which joined to form one major cluster at 32% level of similarity. Also Cichorium spinosum, Crete and Cichorium spinosum, Kythnos, varieties with 100% similarity are synonyms. The similarity indices of the RAPD dendrogram ranged between 30% and 100% averagely high enough to suggest useful variability for genetic diversity and plant breeding.","PeriodicalId":20600,"journal":{"name":"Progress in Nutrition","volume":"20 1","pages":"153-159"},"PeriodicalIF":0.0,"publicationDate":"2018-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45966625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-06DOI: 10.23751/PN.V20I1-S.5798
Saira Tanweer, A. Shehzad, M. S. Butt, M. Shahid
Novel health boosting strategies of the millennium have illuminated phytoceutic as one of the promising therapeutic tool to mitigate various health related disorders. In current research work, indigenously grown ginger variety suravi was evaluated for its anti-ulcerogenic, anti-inflammatory, hepato-protective and nephron-defensive potential. For this purpose, nutraceuticalCSE T1 (ethanol extract, 90 min) and nutraceuticalSFE T2 (3300 psi, 50°C, 3 hr) were given to Sprague Dawley rats via oral gavage according to daily intake values (i.e. 3 and 0.3% respectively). In aspirin induced ulcer, ginger extracts showed 38.02% (T1) and 42.9% (T2) reduction for gastric juice volume, 8.45 (T1) and 17.87% (T2) reduction in gastric juice acidity. However, the ulcer index decreased from 2.63 to 1.97 (T1) and 1.68 (T2). In carrageenan induced inflammation, ginger extracts lessen the paw edema from 0.273 to 0.260 and 0.256 in T1 and T2. In CCl4 induced hepatotoxicity, nutraceuticalCSE showed 12.94% reduction in AST, 4.74% in ALP, 10.13% in ALT and 6.56% in bilirubin, however, the nutraceuticalSFE proved 19.99, 14.88, 18.07 and 10.18% reduction in AST, ALP, ALT and bilirubin, respectively. Similarly, in gentamicin induced nephron toxicity, the decrease rate was 8.94, 8.87 and 9.42% for urea, creatinine and uric acid content, accordingly the urea, creatinine and uric acid level lessen to 14.32, 12.10 and 15.94%, correspondingly in T1 and T2.
{"title":"Probing the therapeutical potential of conventional and supercritical fluid extract of Zingiber officinale to mitigate ulcer, inflammation, hepatotoxicity and nephron toxicity","authors":"Saira Tanweer, A. Shehzad, M. S. Butt, M. Shahid","doi":"10.23751/PN.V20I1-S.5798","DOIUrl":"https://doi.org/10.23751/PN.V20I1-S.5798","url":null,"abstract":"Novel health boosting strategies of the millennium have illuminated phytoceutic as one of the promising therapeutic tool to mitigate various health related disorders. In current research work, indigenously grown ginger variety suravi was evaluated for its anti-ulcerogenic, anti-inflammatory, hepato-protective and nephron-defensive potential. For this purpose, nutraceuticalCSE T1 (ethanol extract, 90 min) and nutraceuticalSFE T2 (3300 psi, 50°C, 3 hr) were given to Sprague Dawley rats via oral gavage according to daily intake values (i.e. 3 and 0.3% respectively). In aspirin induced ulcer, ginger extracts showed 38.02% (T1) and 42.9% (T2) reduction for gastric juice volume, 8.45 (T1) and 17.87% (T2) reduction in gastric juice acidity. However, the ulcer index decreased from 2.63 to 1.97 (T1) and 1.68 (T2). In carrageenan induced inflammation, ginger extracts lessen the paw edema from 0.273 to 0.260 and 0.256 in T1 and T2. In CCl4 induced hepatotoxicity, nutraceuticalCSE showed 12.94% reduction in AST, 4.74% in ALP, 10.13% in ALT and 6.56% in bilirubin, however, the nutraceuticalSFE proved 19.99, 14.88, 18.07 and 10.18% reduction in AST, ALP, ALT and bilirubin, respectively. Similarly, in gentamicin induced nephron toxicity, the decrease rate was 8.94, 8.87 and 9.42% for urea, creatinine and uric acid content, accordingly the urea, creatinine and uric acid level lessen to 14.32, 12.10 and 15.94%, correspondingly in T1 and T2.","PeriodicalId":20600,"journal":{"name":"Progress in Nutrition","volume":"20 1","pages":"255-278"},"PeriodicalIF":0.0,"publicationDate":"2018-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68833694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-06DOI: 10.23751/PN.V20I1-S.6654
Amita Jain, Meerambika Mishra, D. Yadav, Dheeraj Khatarker, Pratiksha Jadaun, Arvind Tiwari, Charu Katare, G. Prasad
Background and Aim: The present study has been undertaken to analyze the antihyperglycemic, antilipidemic and antioxidant potential of Cucurbita ficifolia juice in type 2 diabetic subjects. Methodology: A total 34 subjects were selected for the study out of which 14 subjects were with type 2 diabetes mellitus, 10 subjects were selected as a normal control group and 10 subjects were selected as a normal treated group. The patients had type 2 diabetes for more than 2 years, their mean age was less than 45 years, and subjects were then asked to start the Cucurbita ficifolia juice therapy starting at day 0 and continuing up to 40 day both for diabetic and normal subjects. Fasting blood glucose, glycosylated hemoglobin, lipid profile and anti-oxidant levels were measured two times at 0 days and continuous 40 days Cucurbita ficifolia therapy. Results: This study compared the measurement of glucose levels, lipid biomarkers, antioxidant and lipid peroxidation in all three groups [normal control, normal treated, and type 2 diabetic patients treated], at 0 days and after therapy of 40 days with Cucurbita ficifolia juice. Conclusion: The results suggest that the Cucurbita ficifolia juice therapy in type 2 diabetes may have the potential to regulate hyperglycemia, hypercholesterolemia and enhance the antioxidants enzymes along with reduction of lipid peroxidation.
{"title":"Evaluation of the antihyperglycemic, antilipidemic and antioxidant potential of Cucurbita ficifolia in human type 2 diabetes","authors":"Amita Jain, Meerambika Mishra, D. Yadav, Dheeraj Khatarker, Pratiksha Jadaun, Arvind Tiwari, Charu Katare, G. Prasad","doi":"10.23751/PN.V20I1-S.6654","DOIUrl":"https://doi.org/10.23751/PN.V20I1-S.6654","url":null,"abstract":"Background and Aim: The present study has been undertaken to analyze the antihyperglycemic, antilipidemic and antioxidant potential of Cucurbita ficifolia juice in type 2 diabetic subjects. Methodology: A total 34 subjects were selected for the study out of which 14 subjects were with type 2 diabetes mellitus, 10 subjects were selected as a normal control group and 10 subjects were selected as a normal treated group. The patients had type 2 diabetes for more than 2 years, their mean age was less than 45 years, and subjects were then asked to start the Cucurbita ficifolia juice therapy starting at day 0 and continuing up to 40 day both for diabetic and normal subjects. Fasting blood glucose, glycosylated hemoglobin, lipid profile and anti-oxidant levels were measured two times at 0 days and continuous 40 days Cucurbita ficifolia therapy. Results: This study compared the measurement of glucose levels, lipid biomarkers, antioxidant and lipid peroxidation in all three groups [normal control, normal treated, and type 2 diabetic patients treated], at 0 days and after therapy of 40 days with Cucurbita ficifolia juice. Conclusion: The results suggest that the Cucurbita ficifolia juice therapy in type 2 diabetes may have the potential to regulate hyperglycemia, hypercholesterolemia and enhance the antioxidants enzymes along with reduction of lipid peroxidation.","PeriodicalId":20600,"journal":{"name":"Progress in Nutrition","volume":"20 1","pages":"191-198"},"PeriodicalIF":0.0,"publicationDate":"2018-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49663732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-06DOI: 10.23751/PN.V20I1-S.5669
F. Erman, O. A. Kireçci, A. D. Ozsahin, O. Erman, Tubay Kaya, Ö. Yılmaz
In this study, the effects of goldenberry and lupin on lipid peroxidation and antioxidant system parameters of liver and kidney tissues of streptozotocin-induced diabetic rats were investigated. Type II diabetes was produced in rats by the streptozotocin intraperitoneal injection. Albino rats were divided into four groups, each one containing 10 rats: non-diabetic control group, STZ-Diabetes type II group, STZ-Diabetes+goldenberry type II group, and STZ-Diabetes+lupin type II group. After one week from the injection, goldenberry and lupin were intraperitoneal injected to rats for 2 months. Malondialdehyde, glutathione, cholesterol, and fatty acid levels, which are signs of lipid peroxidation, were measured in these tissues. At the beginning and end of the study, postprandial blood glucose levels and weights of the rats were measured. In type II diabetes, malondialdehyde increased compared to the control group. Glutathione decreased in the other tissues and all of the streptozotocin-induced diabetic groups. In type II diabetes, liver cholesterol levels increased. Treatment with similar doses of goldenberry and lupin extracts significantly reduced postprandial hyperglycemia, oxidative stress, and augmented antioxidant system. The results of the present study showed that the herb suspensions exerted anti-hyperglycemic effects and consequently may alleviative liver and kidney damage caused by streptozotocin-induced diabetes.
{"title":"Effects of Physalis peruviana and Lupinus albus on malondialdehyde, glutathione, cholesterol, vitamins and fatty acid levels in kidney and liver tissues of diabetic rats","authors":"F. Erman, O. A. Kireçci, A. D. Ozsahin, O. Erman, Tubay Kaya, Ö. Yılmaz","doi":"10.23751/PN.V20I1-S.5669","DOIUrl":"https://doi.org/10.23751/PN.V20I1-S.5669","url":null,"abstract":"In this study, the effects of goldenberry and lupin on lipid peroxidation and antioxidant system parameters of liver and kidney tissues of streptozotocin-induced diabetic rats were investigated. Type II diabetes was produced in rats by the streptozotocin intraperitoneal injection. Albino rats were divided into four groups, each one containing 10 rats: non-diabetic control group, STZ-Diabetes type II group, STZ-Diabetes+goldenberry type II group, and STZ-Diabetes+lupin type II group. After one week from the injection, goldenberry and lupin were intraperitoneal injected to rats for 2 months. Malondialdehyde, glutathione, cholesterol, and fatty acid levels, which are signs of lipid peroxidation, were measured in these tissues. At the beginning and end of the study, postprandial blood glucose levels and weights of the rats were measured. In type II diabetes, malondialdehyde increased compared to the control group. Glutathione decreased in the other tissues and all of the streptozotocin-induced diabetic groups. In type II diabetes, liver cholesterol levels increased. Treatment with similar doses of goldenberry and lupin extracts significantly reduced postprandial hyperglycemia, oxidative stress, and augmented antioxidant system. The results of the present study showed that the herb suspensions exerted anti-hyperglycemic effects and consequently may alleviative liver and kidney damage caused by streptozotocin-induced diabetes.","PeriodicalId":20600,"journal":{"name":"Progress in Nutrition","volume":"20 1","pages":"218-230"},"PeriodicalIF":0.0,"publicationDate":"2018-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42583568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-06DOI: 10.23751/PN.V20I1-S.5788
S. Zahoor, M. Babar, M. Javed, T. Hussain
Constipation is a common gastrointestinal complaint in pregnancy that occurs in about 11-40% of the women. It is described by symptoms like pain, distress, straining, hard lumpy stools and a sense of incomplete bowel evacuation. Diet rich in fiber and increased fluid intake is recommended to improve defecation. The use of laxatives and probiotics is also reported to alleviate constipation, though the safety and efficacy of laxatives and probiotics on the mother and fetus need to be established. This review article provides an insight on different factors leading to constipation in pregnancy and evaluation of different remedies used for the treatment of constipation in pregnancy.
{"title":"Constipation in pregnancy: causes and remedies","authors":"S. Zahoor, M. Babar, M. Javed, T. Hussain","doi":"10.23751/PN.V20I1-S.5788","DOIUrl":"https://doi.org/10.23751/PN.V20I1-S.5788","url":null,"abstract":"Constipation is a common gastrointestinal complaint in pregnancy that occurs in about 11-40% of the women. It is described by symptoms like pain, distress, straining, hard lumpy stools and a sense of incomplete bowel evacuation. Diet rich in fiber and increased fluid intake is recommended to improve defecation. The use of laxatives and probiotics is also reported to alleviate constipation, though the safety and efficacy of laxatives and probiotics on the mother and fetus need to be established. This review article provides an insight on different factors leading to constipation in pregnancy and evaluation of different remedies used for the treatment of constipation in pregnancy.","PeriodicalId":20600,"journal":{"name":"Progress in Nutrition","volume":"20 1","pages":"305-311"},"PeriodicalIF":0.0,"publicationDate":"2018-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48370135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-06DOI: 10.23751/PN.V20I1-S.5930
E. Çaçan, K. Kökten, Ömer Kılıç
Fatty acids composition leaves of Satureja hortensis L., Satureja boissieri Hausskn ex Boiss., Thymus kotschyanus Boiss.& Hohen. var. glabrescens Boiss., Thymus kotschyanus Boiss. & Hohen. var. kotschyanus, Thymus hausknechtii Velen., Thymus pubescens Boiss. & Kotschy ex Celak var. pubescens, Thymus fallax Fisch & Mey, Origanum vulgare L. subsp. gracile and Origanum acutidens L., were analyzed by gas chromatography. The fatty acid composition of plants used to this study showed different saturated and unsaturated fatty acid concentrations. The main fatty acids found were Palmitic acid methyl ester (13.49-27.71%), Linoleic acid methyl ester (10.85-19.47%), and Linolenic acid methyl ester (40.68-56.53%); while other fatty acids were found in minor proportions. As a result, all taxa had the highest total unsaturated fatty acid amounts (39.05-73.19%) and the lowest total saturated fatty acid amounts (19.43-51.12%). The highest unsaturated fatty acid determined to Satureja hortensis (73.19%), the lowest to Thymus hausknechtii (39.05%). In this study, palmitic acid methyl ester was found the major saturated fatty acids in all studied taxa.
{"title":"Leaf fatty acid composition of some Lamiaceae taxa from Turkey","authors":"E. Çaçan, K. Kökten, Ömer Kılıç","doi":"10.23751/PN.V20I1-S.5930","DOIUrl":"https://doi.org/10.23751/PN.V20I1-S.5930","url":null,"abstract":"Fatty acids composition leaves of Satureja hortensis L., Satureja boissieri Hausskn ex Boiss., Thymus kotschyanus Boiss.& Hohen. var. glabrescens Boiss., Thymus kotschyanus Boiss. & Hohen. var. kotschyanus, Thymus hausknechtii Velen., Thymus pubescens Boiss. & Kotschy ex Celak var. pubescens, Thymus fallax Fisch & Mey, Origanum vulgare L. subsp. gracile and Origanum acutidens L., were analyzed by gas chromatography. The fatty acid composition of plants used to this study showed different saturated and unsaturated fatty acid concentrations. The main fatty acids found were Palmitic acid methyl ester (13.49-27.71%), Linoleic acid methyl ester (10.85-19.47%), and Linolenic acid methyl ester (40.68-56.53%); while other fatty acids were found in minor proportions. As a result, all taxa had the highest total unsaturated fatty acid amounts (39.05-73.19%) and the lowest total saturated fatty acid amounts (19.43-51.12%). The highest unsaturated fatty acid determined to Satureja hortensis (73.19%), the lowest to Thymus hausknechtii (39.05%). In this study, palmitic acid methyl ester was found the major saturated fatty acids in all studied taxa.","PeriodicalId":20600,"journal":{"name":"Progress in Nutrition","volume":"20 1","pages":"231-236"},"PeriodicalIF":0.0,"publicationDate":"2018-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68833706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-06DOI: 10.23751/PN.V20I1-S.6744
H. Aşkın, B. Yılmaz, S. Bakirci, A. Ayar
Turkey is rich in terms of triterpenes and sterols flora. Triterpenes and sterols hold out for medicinal advances and new drugs. This paper describes a simple, precise, sensitive and specific method for the simultaneous determination of α-amyrin and β-sitosterol in Centranthus longiflorus and Iris taochia by gas chromatography-mass spectrometry (GC-MS) method. The retention times of α-amyrin and β-sitosterol were found 16.1 and 15.1 min, respectively. The linear ranges in this developed method were 1-100 and 5-750 µg/mL for α-amyrin and β-sitosterol, respectively. The intra- and inter-day precisions, expressed as the relative standard deviation (RSD), were less than 4.97 and 4.99%, determined from quality control samples for α-amyrin and β-sitosterol, and accuracy was within 2.00 and 4.60% in terms of relative error, respectively. The percentage recovery obtained for α-amyrin and β-sitosterol were 99.7 and 99.8%, respectively. Limit of detection and quantification for α-amyrin were 5 and 15 ng/mL, for β-sitosterol 50 and 150 ng/mL, respectively. The developed method can be used for routine quality control analysis of α-amyrin and β-sitosterol in dried whole plant of Centranthus longiflorus and Iris taochia.
{"title":"Simultaneous determination of α-amyrin and β-sitosterol in Centranthus longiflorus Stev. Subsp. longiflorus Stev and Iris taochia Woronow ex Grossh by GC-MS method","authors":"H. Aşkın, B. Yılmaz, S. Bakirci, A. Ayar","doi":"10.23751/PN.V20I1-S.6744","DOIUrl":"https://doi.org/10.23751/PN.V20I1-S.6744","url":null,"abstract":"Turkey is rich in terms of triterpenes and sterols flora. Triterpenes and sterols hold out for medicinal advances and new drugs. This paper describes a simple, precise, sensitive and specific method for the simultaneous determination of α-amyrin and β-sitosterol in Centranthus longiflorus and Iris taochia by gas chromatography-mass spectrometry (GC-MS) method. The retention times of α-amyrin and β-sitosterol were found 16.1 and 15.1 min, respectively. The linear ranges in this developed method were 1-100 and 5-750 µg/mL for α-amyrin and β-sitosterol, respectively. The intra- and inter-day precisions, expressed as the relative standard deviation (RSD), were less than 4.97 and 4.99%, determined from quality control samples for α-amyrin and β-sitosterol, and accuracy was within 2.00 and 4.60% in terms of relative error, respectively. The percentage recovery obtained for α-amyrin and β-sitosterol were 99.7 and 99.8%, respectively. Limit of detection and quantification for α-amyrin were 5 and 15 ng/mL, for β-sitosterol 50 and 150 ng/mL, respectively. The developed method can be used for routine quality control analysis of α-amyrin and β-sitosterol in dried whole plant of Centranthus longiflorus and Iris taochia.","PeriodicalId":20600,"journal":{"name":"Progress in Nutrition","volume":"20 1","pages":"209-217"},"PeriodicalIF":0.0,"publicationDate":"2018-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43668855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-06DOI: 10.23751/PN.V20I1-S.5311
E. Bursal, R. Boğa
Objectives: The leaves of oak trees have been boiled in water and the extracts have been evaporated to produce oak molasses. Colloidal precipitants have been widely used as dietary molasses since have very effective antiseptic functions as alternative medicine for ages in some parts of the world. Present study aimed to investigate the biological activities of oak products for elucidating the scientific basis of alternative medicine. Thus, antioxidant activities of ethanol and water extracts of acorn, leaves and molasses of oak trees (Quercus robur subsp. pedunculiflora) were evaluated. Results: We determined antioxidant activities of extracts by different in vitro methods including DPPH free radical scavenging, ABTS•+ radical scavenging, total antioxidant activity determination by ferric thiocyanate method, reducing power by FRAP methods, separately. The results were compared with BHA, BHT and ascorbic acid as standard antioxidant compounds. The acorn seed extract, which presented the highest antioxidant potential, was the most effective scavenger against DPPH free radical. Also, phenolic compounds of the molasses were identified by UHPLC-ESI-MS/MS. The highest phenolic compound was quinic acid (4556 ± 219 ppb). Conclusion: Acorns and leaves of oak trees (Quercus robur subsp. pedunculiflora), as well as traditional oak molasses” have high antioxidant potential for the food industry as a food ingredient to produce functional food products.
{"title":"Polyphenols analysed by UHPLC-ESI-MS/MS and antioxidant activities of molasses, acorn and leaves of oak (Quercus robur subsp. pedunculiflora)","authors":"E. Bursal, R. Boğa","doi":"10.23751/PN.V20I1-S.5311","DOIUrl":"https://doi.org/10.23751/PN.V20I1-S.5311","url":null,"abstract":"Objectives: The leaves of oak trees have been boiled in water and the extracts have been evaporated to produce oak molasses. Colloidal precipitants have been widely used as dietary molasses since have very effective antiseptic functions as alternative medicine for ages in some parts of the world. Present study aimed to investigate the biological activities of oak products for elucidating the scientific basis of alternative medicine. Thus, antioxidant activities of ethanol and water extracts of acorn, leaves and molasses of oak trees (Quercus robur subsp. pedunculiflora) were evaluated. Results: We determined antioxidant activities of extracts by different in vitro methods including DPPH free radical scavenging, ABTS•+ radical scavenging, total antioxidant activity determination by ferric thiocyanate method, reducing power by FRAP methods, separately. The results were compared with BHA, BHT and ascorbic acid as standard antioxidant compounds. The acorn seed extract, which presented the highest antioxidant potential, was the most effective scavenger against DPPH free radical. Also, phenolic compounds of the molasses were identified by UHPLC-ESI-MS/MS. The highest phenolic compound was quinic acid (4556 ± 219 ppb). Conclusion: Acorns and leaves of oak trees (Quercus robur subsp. pedunculiflora), as well as traditional oak molasses” have high antioxidant potential for the food industry as a food ingredient to produce functional food products.","PeriodicalId":20600,"journal":{"name":"Progress in Nutrition","volume":"20 1","pages":"167-175"},"PeriodicalIF":0.0,"publicationDate":"2018-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44762182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-06DOI: 10.23751/PN.V20I1-S.6686
S. Kazemiani, A. Motallebi-Azar, J. Panahandeh, S. Mokhtarzadeh, F. A. Özdemir
The present investigation was conducted to develop a protocol for rapid shoot proliferation of potato by MS medium and different concentrations of plant growth regulators. In this study, the node explant was cultured in MS media supplemented with different concentrations of thiamine-HCl (0.1 and 10 mg.l-1), Pyridoxine- HCl, Nicotinic acid (each 0.5 and 50 mg.l-1) and BAP (0.0, 2 and 4 mg.l-1) for shoot proliferation. Maximum number of lateral shoots was observed in MS medium containing 50 mg.l-1 Nicotinic acid, 50 mg.l-1Pyridoxine- HCl, 10 mg.l-1Thiamine-HCl and 4 mg.l-1BAP. Also, maximum percentage of root and callus formation was observed in combination of MS include vitamins without BAP, the present study describes an efficient method for in vitro shoot proliferation of potato cultivars which could be considered for large scale multiplication and propagation of this important vegetable crop.
{"title":"Shoot proliferation from potato (Solanum tuberosum cv. Agria) under different concentration of MS include vitamins and BAP medium","authors":"S. Kazemiani, A. Motallebi-Azar, J. Panahandeh, S. Mokhtarzadeh, F. A. Özdemir","doi":"10.23751/PN.V20I1-S.6686","DOIUrl":"https://doi.org/10.23751/PN.V20I1-S.6686","url":null,"abstract":"The present investigation was conducted to develop a protocol for rapid shoot proliferation of potato by MS medium and different concentrations of plant growth regulators. In this study, the node explant was cultured in MS media supplemented with different concentrations of thiamine-HCl (0.1 and 10 mg.l-1), Pyridoxine- HCl, Nicotinic acid (each 0.5 and 50 mg.l-1) and BAP (0.0, 2 and 4 mg.l-1) for shoot proliferation. Maximum number of lateral shoots was observed in MS medium containing 50 mg.l-1 Nicotinic acid, 50 mg.l-1Pyridoxine- HCl, 10 mg.l-1Thiamine-HCl and 4 mg.l-1BAP. Also, maximum percentage of root and callus formation was observed in combination of MS include vitamins without BAP, the present study describes an efficient method for in vitro shoot proliferation of potato cultivars which could be considered for large scale multiplication and propagation of this important vegetable crop.","PeriodicalId":20600,"journal":{"name":"Progress in Nutrition","volume":"20 1","pages":"160-166"},"PeriodicalIF":0.0,"publicationDate":"2018-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48065845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-06DOI: 10.23751/PN.V20I1-S.6394
S. Rana, Meerambika Mishra, D. Yadav, S. Subramani, Charu Katare, G. Prasad
Honey often referred to as ‘the drink of the gods’, is naturally sweet and a substantially rich source of carbohydrates, amino acids and antioxidants. In spite of being rich in carbohydrates, honey has a low glycemic index and therefore effectively used as a dietary compliance by diabetics. The fructose content of honey has hepatoprotective capability, while the antioxidants present in it provide effective protection against oxidative damage. The therapeutic attribute of honey makes it the food of choice even in infants and diabetics. Honey is endowed with antioxidant, immune modulating, and wound healing, anti-inflammatory, therapeutic, nutritional, antimicrobial and antidiabetic qualities. The present review aims at discussing these capabilities of honey with special reference to its antidiabetic benefits under one dome.
{"title":"Medicinal uses of honey: a review on its benefits to human health","authors":"S. Rana, Meerambika Mishra, D. Yadav, S. Subramani, Charu Katare, G. Prasad","doi":"10.23751/PN.V20I1-S.6394","DOIUrl":"https://doi.org/10.23751/PN.V20I1-S.6394","url":null,"abstract":"Honey often referred to as ‘the drink of the gods’, is naturally sweet and a substantially rich source of carbohydrates, amino acids and antioxidants. In spite of being rich in carbohydrates, honey has a low glycemic index and therefore effectively used as a dietary compliance by diabetics. The fructose content of honey has hepatoprotective capability, while the antioxidants present in it provide effective protection against oxidative damage. The therapeutic attribute of honey makes it the food of choice even in infants and diabetics. Honey is endowed with antioxidant, immune modulating, and wound healing, anti-inflammatory, therapeutic, nutritional, antimicrobial and antidiabetic qualities. The present review aims at discussing these capabilities of honey with special reference to its antidiabetic benefits under one dome.","PeriodicalId":20600,"journal":{"name":"Progress in Nutrition","volume":"20 1","pages":"5-14"},"PeriodicalIF":0.0,"publicationDate":"2018-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45358915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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