Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.013
R. E. Putra, Rilvi Abilia Tesa, M. Rosmiati, A. Dwiartama
{"title":"Application of Bokashi, Vermicompost, and Residue of Coffee Testa Bioconversion by Black Soldier Fly (Hermetia illucens) on the Production of Japanese Cucumber (Luffa acutangula)","authors":"R. E. Putra, Rilvi Abilia Tesa, M. Rosmiati, A. Dwiartama","doi":"10.2991/absr.k.210621.013","DOIUrl":"https://doi.org/10.2991/absr.k.210621.013","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74080038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.012
P. H. Putri, N. Nugrahaeni
Cowpea is an underutilized legume that has been cultivating in Indonesia. But, the availability of cowpea variety is limited. Cowpea breeding program is started with gene source characterization of germplasm. This research aims to study the yield and its supported component variability of cowpea germplasm. One hundred and fifty cowpea accessions were planted using randomized block design with two replications. Observation was done for days of flowering, days of maturity, plant height, number of branches, number of pods per plant, pod length, number of seeds per pod, seed weights per plant, 100 seeds weight, and seed weights per plot. Statistic descriptive, analysis of variance, and was analyzed with MSTAT 1.4 version software, while path analysis was done with SPSS 19 version. Days of flowering was ranged 32-53 das, days of maturity 60-79 das, plant height 16-51.3 cm, number of branches 0-5, number of pods per plant 13-93, pod length 9.1-22.8 cm, number of seeds per pod 12-20, seed weight 7.47-76.17 g per plant, 100 seeds weight 4.19-18.5 g, and seed weight per plot 45.61346.58 g. There were significant differences between the genotype in leaf length x width, plant height, pod length, number of pods per plant, weight of 100 seeds, and seed weight per plot. All of the observed characters were correlated highly significant to yield, except days of maturity, leaf length x width, and pod length which correlated significantly, also number of branches and number of seeds per pod which not correlated significantly to yield. Selection was held for yield character using 20% selection index and there were 19 accessions selected, with 269.58-326.48 g seed yield per plot.
{"title":"Cowpea [Vigna unguiculata (L.) Walp.] Yield Variance and Supported Character","authors":"P. H. Putri, N. Nugrahaeni","doi":"10.2991/absr.k.210621.012","DOIUrl":"https://doi.org/10.2991/absr.k.210621.012","url":null,"abstract":"Cowpea is an underutilized legume that has been cultivating in Indonesia. But, the availability of cowpea variety is limited. Cowpea breeding program is started with gene source characterization of germplasm. This research aims to study the yield and its supported component variability of cowpea germplasm. One hundred and fifty cowpea accessions were planted using randomized block design with two replications. Observation was done for days of flowering, days of maturity, plant height, number of branches, number of pods per plant, pod length, number of seeds per pod, seed weights per plant, 100 seeds weight, and seed weights per plot. Statistic descriptive, analysis of variance, and was analyzed with MSTAT 1.4 version software, while path analysis was done with SPSS 19 version. Days of flowering was ranged 32-53 das, days of maturity 60-79 das, plant height 16-51.3 cm, number of branches 0-5, number of pods per plant 13-93, pod length 9.1-22.8 cm, number of seeds per pod 12-20, seed weight 7.47-76.17 g per plant, 100 seeds weight 4.19-18.5 g, and seed weight per plot 45.61346.58 g. There were significant differences between the genotype in leaf length x width, plant height, pod length, number of pods per plant, weight of 100 seeds, and seed weight per plot. All of the observed characters were correlated highly significant to yield, except days of maturity, leaf length x width, and pod length which correlated significantly, also number of branches and number of seeds per pod which not correlated significantly to yield. Selection was held for yield character using 20% selection index and there were 19 accessions selected, with 269.58-326.48 g seed yield per plot.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90147612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Violin Crab (Uca) is type of crab living in mangrove habitat as a detrivore. In Bengkulu, the mangrove forest zone locates in many areas, including Kahyapu, Enggano Island and Gulf of Muaro Labu Nawi, Pulau Baai, Bengkulu City. The objective of this experiment was to investigate the pattern of Violin Crab distribution at mangrove forest in Kahyapu and Gulf of Muaro. The methodology used in this experiment, carried out from 2017 to 2018, was a survey done by random sampling. The samples were taken from 2 stations at 10 plots, whose size was1m x 1m each. The variable measured included the crab pattern of distribution and abiotic factor which might affect the growth of the crabs, such as water pH, temperature, and salinity Muaro Labu Nawi. However, the total Biola crab in these two habitats was 11, including Uca annulipes, Uca boninensis,Uca coarcata, Uca crassipes, Uca dussemiert, Uca forcipata, Uca perplexa, Uca rosea, Uca tetragon, Uca triangularis, and Uca vacans. The violin crab inhabitating Kahyapu showed random pattern of distribution while those in the Gulf of Muaro Labu Nawi had clustered and random distribution. Moreover, Kahyapu habitat had water temperature of 27–30 C, solution pH of 6.4–6.7, and salinity level of 26–28%. On the other hand, the Gulf of Muaro Labu Nawi had water temperature of 27–31 C, pH of 6.5–6.9, and 26–31% of salinity level.
小提琴蟹(Uca)是一种生活在红树林栖息地的腐食蟹。在明古鲁，红树林区域分布在许多地区，包括Kahyapu, Enggano岛和Muaro Labu Nawi湾，Pulau Baai，明古鲁市。本试验旨在研究卡亚普和穆阿罗湾红树林小提琴蟹的分布格局。本实验于2017年至2018年进行，采用随机抽样的调查方法。样本取自10个样地的2个站点，每个样地的大小为1m × 1m。测量的变量包括蟹的分布模式和可能影响蟹生长的非生物因素，如水体pH、温度和盐度。而在这两个生境中，斑蟹总数为11只，分别为环状斑蟹、boninensis斑蟹、coarcata斑蟹、crassipes斑蟹、dussemiert斑蟹、forcipatata斑蟹、perplexa斑蟹、rosea斑蟹、tetragon斑蟹、triangularis斑蟹和vacans斑蟹。栖息在卡亚普的小提琴蟹呈随机分布，而在穆阿罗拉布纳威湾的小提琴蟹呈聚集性随机分布。喀亚普生境水温为27 ~ 30℃，pH值为6.4 ~ 6.7，盐度为26 ~ 28%。另一方面，Muaro Labu Nawi湾的水温为27-31℃，pH值为6.5-6.9，盐度为26-31%。
{"title":"The Type and Distribution of Violin Crab (Uca) at Mangrove Forest of Kahyapu, Enggano Island and The Gulf of Muaro, Labu Nawi, Bengkulu City","authors":"Novia Duya, Jefri Novriansyah, Rista Noveria, Darmi","doi":"10.2991/absr.k.210621.028","DOIUrl":"https://doi.org/10.2991/absr.k.210621.028","url":null,"abstract":"Violin Crab (Uca) is type of crab living in mangrove habitat as a detrivore. In Bengkulu, the mangrove forest zone locates in many areas, including Kahyapu, Enggano Island and Gulf of Muaro Labu Nawi, Pulau Baai, Bengkulu City. The objective of this experiment was to investigate the pattern of Violin Crab distribution at mangrove forest in Kahyapu and Gulf of Muaro. The methodology used in this experiment, carried out from 2017 to 2018, was a survey done by random sampling. The samples were taken from 2 stations at 10 plots, whose size was1m x 1m each. The variable measured included the crab pattern of distribution and abiotic factor which might affect the growth of the crabs, such as water pH, temperature, and salinity Muaro Labu Nawi. However, the total Biola crab in these two habitats was 11, including Uca annulipes, Uca boninensis,Uca coarcata, Uca crassipes, Uca dussemiert, Uca forcipata, Uca perplexa, Uca rosea, Uca tetragon, Uca triangularis, and Uca vacans. The violin crab inhabitating Kahyapu showed random pattern of distribution while those in the Gulf of Muaro Labu Nawi had clustered and random distribution. Moreover, Kahyapu habitat had water temperature of 27–30 C, solution pH of 6.4–6.7, and salinity level of 26–28%. On the other hand, the Gulf of Muaro Labu Nawi had water temperature of 27–31 C, pH of 6.5–6.9, and 26–31% of salinity level.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87970395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.054
S. W. R. Hilia, Abinawanto, A. Kristanto
{"title":"Effect of Honey Supplementation on Sperm Abnormality in Sperm Preservation of Brek Fish Systomous orphoides","authors":"S. W. R. Hilia, Abinawanto, A. Kristanto","doi":"10.2991/absr.k.210621.054","DOIUrl":"https://doi.org/10.2991/absr.k.210621.054","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90981665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.074
B. N. Hidayah, N. Herawati, A. Aisah, M. Syafwan
{"title":"White Rot Disease on Garlic Crop:","authors":"B. N. Hidayah, N. Herawati, A. Aisah, M. Syafwan","doi":"10.2991/absr.k.210621.074","DOIUrl":"https://doi.org/10.2991/absr.k.210621.074","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91210286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.016
Y. Sondang, R. Siregar, K. Anty
Endophytic bacteria are bacteria that live in plant tissue without destroying the host plant. The presence of endophytic bacteria in plant tissue can produce phytohormones to stimulate growth and increase plant resistance to disease. The purpose of this study is to identify endophytic bacteria from the root tissue of maize plants treated with water hyacinth biofertilizer and to calculate the number of bacterial populations. The research was conducted at the Experimental Garden and Laboratory of the Payakumbuh State Agricultural Polytechnic. The treatments of biofertilizer were carried out by immersing the seeds in biofertilizer 4% solution for 12 hours, then maize plants were treated with a biofertilizer in the planting hole, and the age of the plants was 14, 28, 42, 56 days after planting. Observation parameters were carried out on endophytic bacteria of the maize plant and the number of bacterial populations. Identification of bacterial morphology based on colony shape, colony edges, colony elevation, surface, color, bacterial density, and gram staining. Bacterial species were identified using molecular analysis based on the 16S rRNA gene fragment. The identification results of maize root endophytic bacteria found 4 dominant bacterial with several bacterial populations species such as B. paramycoides 12,1 x 10 CFU/ml, P. aeruginosa 4,3 x 10 CFU/ml, B. subtilis 9,4 x 10 CFU/ml, and B. licheniformis 5,95 x 10 CFU/ml.
{"title":"The Study Application of Water Hyacinth Biofertilizer Towards the Existence of Endophytic Bacteria in Maize Plant","authors":"Y. Sondang, R. Siregar, K. Anty","doi":"10.2991/absr.k.210621.016","DOIUrl":"https://doi.org/10.2991/absr.k.210621.016","url":null,"abstract":"Endophytic bacteria are bacteria that live in plant tissue without destroying the host plant. The presence of endophytic bacteria in plant tissue can produce phytohormones to stimulate growth and increase plant resistance to disease. The purpose of this study is to identify endophytic bacteria from the root tissue of maize plants treated with water hyacinth biofertilizer and to calculate the number of bacterial populations. The research was conducted at the Experimental Garden and Laboratory of the Payakumbuh State Agricultural Polytechnic. The treatments of biofertilizer were carried out by immersing the seeds in biofertilizer 4% solution for 12 hours, then maize plants were treated with a biofertilizer in the planting hole, and the age of the plants was 14, 28, 42, 56 days after planting. Observation parameters were carried out on endophytic bacteria of the maize plant and the number of bacterial populations. Identification of bacterial morphology based on colony shape, colony edges, colony elevation, surface, color, bacterial density, and gram staining. Bacterial species were identified using molecular analysis based on the 16S rRNA gene fragment. The identification results of maize root endophytic bacteria found 4 dominant bacterial with several bacterial populations species such as B. paramycoides 12,1 x 10 CFU/ml, P. aeruginosa 4,3 x 10 CFU/ml, B. subtilis 9,4 x 10 CFU/ml, and B. licheniformis 5,95 x 10 CFU/ml.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80321222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.021
E. Widjaja, B. N. Utomo
{"title":"Genetic Resources of Sambar Deer (Cervus unicolor) and its Conservation Efforts in Lamandau Regency, Central Kalimantan","authors":"E. Widjaja, B. N. Utomo","doi":"10.2991/absr.k.210621.021","DOIUrl":"https://doi.org/10.2991/absr.k.210621.021","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86342805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.014
R. Budiono, Handoko, G. Susanto, K. Noerwijati
Soybean is an important food commodity that has very large market potential and its needs continue to increase. For the sake of achieving self-sufficiency in soybeans, one important factor is the availability of scatter seeds. Provision of appropriate soybean seeds has a strategic role as a means of supporting increased soybean production. In the field, the seed flow system in Mojokerto Regency, East Java Province, is still facing problems, namely the absence of seed channels for rainy season cultivation in dry land so that it cannot grow a sustainable "Jabalsim" system. For this reason, this activity aims to 1) produce superior soybean seeds according to farmers' preferences to meet market demand, 2) grow seed producers in soybean development areas to ensure adequate supply and distribution of quality seeds of superior varieties, and 3) as well as assist in the application of technological innovations to reduce the yield gap. The results showed that the activity of seed production in Wuwut Village, Mojoanyar Sub-District, Mojokerto District could be categorized as independent. Indicators of independence are: 1) Establishment of a seed producer on behalf of Arifin with a certificate of breeder as Extention Seed producer number 030 / UPT.PSBTPH / MJK / VII / 2019, 2) Superior Varieties of Soybean has been adopted and developed (Dega I, Devon I, Deja II) at the research location even outside the research area, 3) Availability of seeds at all times for the needs of members, and 4) the emergence of a soybean seedling business that has partnered with seed providers. To maintain the continuity of the seeds producer that have been formed, monitoring of their business developments on a regular basis was needed. In addition, in the future, it is necessary to provide access to seed programs that are assisted as one of the seed providers and provide physical infrastructure support so that the speed and volume of seed production can increase.
大豆是一种重要的粮食商品，市场潜力巨大，需求量持续增加。为了实现大豆的自给自足，一个重要因素是分散种子的可用性。提供适当的大豆种子作为支持增加大豆产量的一种手段具有战略作用。在田间，东爪哇省Mojokerto Regency的种子流动系统仍然面临问题，即在旱地缺乏雨季种植的种子渠道，因此无法种植可持续的“Jabalsim”系统。因此，本活动的目的是:1)根据农民的喜好生产优质大豆种子，满足市场需求;2)在大豆开发区培育种子生产者，保证优质品种种子的充足供应和分配;3)协助应用技术创新，缩小产量差距。结果表明:莫乔克托区莫乔亚尔街道五伍特村种子生产活动可归为独立活动;独立性指标为:1)代表Arifin成立一家种子生产商，并持有育种者证书，编号030 / UPT。PSBTPH / MJK / VII / 2019, 2)即使在研究区域之外，也在研究地点采用和开发了大豆优良品种(Dega I, Devon I, Deja II)， 3)随时提供种子以满足成员的需求，4)出现了与种子供应商合作的大豆幼苗业务。为了保持已形成的种子生产商的连续性，需要定期监测其业务发展情况。此外，在未来，有必要提供种子项目，作为种子提供者之一，并提供物理基础设施支持，以提高种子生产的速度和数量。
{"title":"Seed Independence Efforts in Mojokerto District, East Java, to Support the Soybean Self-Sufficiency Program","authors":"R. Budiono, Handoko, G. Susanto, K. Noerwijati","doi":"10.2991/absr.k.210621.014","DOIUrl":"https://doi.org/10.2991/absr.k.210621.014","url":null,"abstract":"Soybean is an important food commodity that has very large market potential and its needs continue to increase. For the sake of achieving self-sufficiency in soybeans, one important factor is the availability of scatter seeds. Provision of appropriate soybean seeds has a strategic role as a means of supporting increased soybean production. In the field, the seed flow system in Mojokerto Regency, East Java Province, is still facing problems, namely the absence of seed channels for rainy season cultivation in dry land so that it cannot grow a sustainable \"Jabalsim\" system. For this reason, this activity aims to 1) produce superior soybean seeds according to farmers' preferences to meet market demand, 2) grow seed producers in soybean development areas to ensure adequate supply and distribution of quality seeds of superior varieties, and 3) as well as assist in the application of technological innovations to reduce the yield gap. The results showed that the activity of seed production in Wuwut Village, Mojoanyar Sub-District, Mojokerto District could be categorized as independent. Indicators of independence are: 1) Establishment of a seed producer on behalf of Arifin with a certificate of breeder as Extention Seed producer number 030 / UPT.PSBTPH / MJK / VII / 2019, 2) Superior Varieties of Soybean has been adopted and developed (Dega I, Devon I, Deja II) at the research location even outside the research area, 3) Availability of seeds at all times for the needs of members, and 4) the emergence of a soybean seedling business that has partnered with seed providers. To maintain the continuity of the seeds producer that have been formed, monitoring of their business developments on a regular basis was needed. In addition, in the future, it is necessary to provide access to seed programs that are assisted as one of the seed providers and provide physical infrastructure support so that the speed and volume of seed production can increase.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84658568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.062
Jarulis, Melisa Mayang Sari, R. H. Wibowo
The study aimed to describe the genetic characteristic, single nucleotide polymorphism, genetic distance, and phylogenetic of mountain white-eye Zosterops montanus based on mitochondrial DNA CO1 gene and carried out in March-June 2020. A sample of the bird was taken from Pasar Minggu, Bengkulu city. The blood sample was taken from the pectoral vein, and the DNA genome was isolated using DNeasy® Blood and Tissue Kit based on Spin-Column Protocol procedure, Qiagen. The CO1 gene was amplified using a polymerase chain reaction technique, electrophoresis on 1.2% agarose gel, and visualized with a UV transilluminator. The results showed that the gene size of CO1 mitochondrial DNA from Z. montanus (n=5) is 751 bp, obtained conservative sites (C) 745 sites, variations (V) six sites, the parsimony of information (Pi) four sites, and singleton sites (S) two sites. The highest nucleotide base composition was cytosine (C) and the lowest guanine (G), while the highest nucleotide combined composition of adenine-thymine (AT) and the lowest guanine-cytosine (GC). We found six single nucleotide polymorphisms were evenly distributed between 9-732 sites. Average intraspecific genetic distance was 0.4%, interspecific genetic distance 4%, and between groups 1 and 2 separated from the outgroup (group 3) with a genetic distance of 12.5%.
{"title":"DNA Barcode of Mountain White-Eye Zosterops montanus (Aves: Zosteropidae) Based on Mitochondrial DNA Cytochrome Oxidase Sub Unit I","authors":"Jarulis, Melisa Mayang Sari, R. H. Wibowo","doi":"10.2991/absr.k.210621.062","DOIUrl":"https://doi.org/10.2991/absr.k.210621.062","url":null,"abstract":"The study aimed to describe the genetic characteristic, single nucleotide polymorphism, genetic distance, and phylogenetic of mountain white-eye Zosterops montanus based on mitochondrial DNA CO1 gene and carried out in March-June 2020. A sample of the bird was taken from Pasar Minggu, Bengkulu city. The blood sample was taken from the pectoral vein, and the DNA genome was isolated using DNeasy® Blood and Tissue Kit based on Spin-Column Protocol procedure, Qiagen. The CO1 gene was amplified using a polymerase chain reaction technique, electrophoresis on 1.2% agarose gel, and visualized with a UV transilluminator. The results showed that the gene size of CO1 mitochondrial DNA from Z. montanus (n=5) is 751 bp, obtained conservative sites (C) 745 sites, variations (V) six sites, the parsimony of information (Pi) four sites, and singleton sites (S) two sites. The highest nucleotide base composition was cytosine (C) and the lowest guanine (G), while the highest nucleotide combined composition of adenine-thymine (AT) and the lowest guanine-cytosine (GC). We found six single nucleotide polymorphisms were evenly distributed between 9-732 sites. Average intraspecific genetic distance was 0.4%, interspecific genetic distance 4%, and between groups 1 and 2 separated from the outgroup (group 3) with a genetic distance of 12.5%.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83930081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.022
E. Riandini, Dhiatama Tauhida Nisa, Della Septa Sari
Hospital wastewater can contain pathogenic microorganisms and harmful chemicals that could affect the environment and the organism around. Testing the quality of wastewater is necessary to determine the level of pathogenic microorganisms or organic and inorganic compounds by testing with physics, chemistry, and biologcal parameters. These tests are performed to identify the quality of wastewater and the factors that influence it, as well as to know the techniques and parameters used in the quality analysis of wastewater. The quality analysis of wastewater using three test of parameters, which include temperature test and TSS (Total Suspended Solid) wich contitudes the physics parameter (14 mg/L), an inorganic chemical parameter that consist the test of Ph (7.51), iron (Fe)(0.018 mg/L), manganese (Mn)( 0.013 mg/L), copper (Cu) (2 mg/L), nitrite (NO2) (0.008 mg/L), sulfide (S) (0.005 mg/L), BOD (Biochemical Oxygen Demand) (25 mg/L), COD (Chemical Oxygen Demand) (55 mg/L), oils and fats, MBAS (Methylene Blue Active Surfactant) (0.306 mg/L), ammonia (NH3-N) (0.860 mg/L), and the microbiology parameter which is total coliform test (2940 MPN/100 ml). These tests are performed according to Indonesian national standards (SNI) and work instructions of environmental laboratory (IK LL). Result from the test showed that quality value of the wastewater analysis does not exceed the quality standards from PERMEN LH NO 5 TH 2014 and PERMEN LH RI No. P.68/Menlhk-Setjen/2016. Based on the result showed that the intalation of wastewater treatment work properly according to the quality standard from the government.
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