Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.086
S. Anwar, R. Astuti, D. Solihin
{"title":"Activity of Ethanol-derived Fraction of Clove Leaves and Eugenol Compound as Antiaging Agent in the Yeast Model Organism Schizosaccharomyces pombe","authors":"S. Anwar, R. Astuti, D. Solihin","doi":"10.2991/absr.k.210621.086","DOIUrl":"https://doi.org/10.2991/absr.k.210621.086","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72954011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.020
Darmi, Rizwar, Helmiyetti
Earthworms are an important component of natural and agricultural land ecosystems. The existence of earthworms in agricultural land can increase soil fertility and also support a sustainable agricultural system. This study aims to determine the abundance and distribution patterns of megadrilli earthworms in the Bengkulu Kabawetan Tea Plantation. Determination of the research location used the stratified sampling method which consisted of altitude strata, namely an altitude of 800, 900, and 1000 meters above sea level. The samples of earthworms were taken using the quadratic method (30 x 30 x 20 cm) and the hand sorting method as many as 15 plots for each location. The data were analyzed for population densities and distribution patterns using the Morisita Index formula. The results showed that only one species of megadrilli earthworm was found Pontoscolex corethrurus in tea plantations. The highest earthworm population density was at a location of 1000 m above sea level (89.66 individuals m), followed by a location of 900 m (70.33 individuals m), and the lowest density was at a location of 800 m (24.44 individuals m). Statistically, it showed that the densities of the earthworm population at an altitude of 1000 m and 900 m were not significantly different, but it was significantly different from an altitude of 800 m. The distribution pattern of megadrilli earthworms based on the Morisita Index value showed that the three locations have a clustered pattern.
{"title":"Abundance and Distribution Patterns of Megadrilli Earthworms at Different Altitude in Kabawetan Tea Plantation, Bengkulu","authors":"Darmi, Rizwar, Helmiyetti","doi":"10.2991/absr.k.210621.020","DOIUrl":"https://doi.org/10.2991/absr.k.210621.020","url":null,"abstract":"Earthworms are an important component of natural and agricultural land ecosystems. The existence of earthworms in agricultural land can increase soil fertility and also support a sustainable agricultural system. This study aims to determine the abundance and distribution patterns of megadrilli earthworms in the Bengkulu Kabawetan Tea Plantation. Determination of the research location used the stratified sampling method which consisted of altitude strata, namely an altitude of 800, 900, and 1000 meters above sea level. The samples of earthworms were taken using the quadratic method (30 x 30 x 20 cm) and the hand sorting method as many as 15 plots for each location. The data were analyzed for population densities and distribution patterns using the Morisita Index formula. The results showed that only one species of megadrilli earthworm was found Pontoscolex corethrurus in tea plantations. The highest earthworm population density was at a location of 1000 m above sea level (89.66 individuals m), followed by a location of 900 m (70.33 individuals m), and the lowest density was at a location of 800 m (24.44 individuals m). Statistically, it showed that the densities of the earthworm population at an altitude of 1000 m and 900 m were not significantly different, but it was significantly different from an altitude of 800 m. The distribution pattern of megadrilli earthworms based on the Morisita Index value showed that the three locations have a clustered pattern.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72650071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.023
Fatimatuzzahra, Jarulis, R. Supriati
BAAI Island has a coastal forest formed from various communities and vegetation structures, which have functions including as a tourist area, a source of food, and disaster mitigation. The aim of this research was to find out the composition, structure and diversity of coastal vegetation on Baai Island area, west of Sumber Jaya Village, Kampung Melayu District, Bengkulu City. Sampling was taken using the transect line plot method with a size of 20 x 20 m for trees, 10 x 10 m for poles, a plot size of 5 x 5 m for saplings. The results showed that the composition of the coastal vegetation at the tree level found 11 families, with a total of 11 species. 5 species came from the pole level, and 4 species with a total of 11 individuals from the sapling level. The species of Casuarina equisetifolia obtained the highest value index of importance at the tree level, while the species Ardisia elliptica had the important value index for the stakes and saplings. The results of the calculation of the Shannon-Wiener diversity index (H'), of all growth levels show a value <1.0 which means low diversity, less stable.
{"title":"Coastal Vegetation Diversity on Baai Island, Kampung Melayu District, Bengkulu City","authors":"Fatimatuzzahra, Jarulis, R. Supriati","doi":"10.2991/absr.k.210621.023","DOIUrl":"https://doi.org/10.2991/absr.k.210621.023","url":null,"abstract":"BAAI Island has a coastal forest formed from various communities and vegetation structures, which have functions including as a tourist area, a source of food, and disaster mitigation. The aim of this research was to find out the composition, structure and diversity of coastal vegetation on Baai Island area, west of Sumber Jaya Village, Kampung Melayu District, Bengkulu City. Sampling was taken using the transect line plot method with a size of 20 x 20 m for trees, 10 x 10 m for poles, a plot size of 5 x 5 m for saplings. The results showed that the composition of the coastal vegetation at the tree level found 11 families, with a total of 11 species. 5 species came from the pole level, and 4 species with a total of 11 individuals from the sapling level. The species of Casuarina equisetifolia obtained the highest value index of importance at the tree level, while the species Ardisia elliptica had the important value index for the stakes and saplings. The results of the calculation of the Shannon-Wiener diversity index (H'), of all growth levels show a value <1.0 which means low diversity, less stable.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85579244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.081
Masrukhin, S. Saputra, Syaiful Rizal, P. S. Sushadi, N. Supriatna
{"title":"Identification and Antibiotic Resistance Profile of Bacteria From Fruit Bat (Chironax melanocephalus)","authors":"Masrukhin, S. Saputra, Syaiful Rizal, P. S. Sushadi, N. Supriatna","doi":"10.2991/absr.k.210621.081","DOIUrl":"https://doi.org/10.2991/absr.k.210621.081","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89294106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.060
Hery Haryanto, S. Nurliana, Syariffuddin Syarifuddin
The objective of this research was to determine the effectiveness of erythrocyte immobilisation with total protein extracted from gnetum seeds on a variety of solid media, i.e., filter papers, doff plastics, and nitrocellulose membranes. Old gnetum seeds were ground to make powders. The powders were homogenized in ml PBS buffer containing anti-protease PMSF in a cool room. The homogenate was centrifuged in order to separate supernatant from pellet. Solid media were punched with a perforator, so the results were discs of papers, plastics, and nitrocellulose membranes. The solid media perforated discs were then dipped in gnetum total protein supernatants. The dipped discs were air-dried for a while. Human erythrocytes of all ABO groups were washed three times and diluted to suspension of 20% (v/v) in NaCl 0,9% (w/v). A small drop of erythrocyte suspension was put in middle of each solid media disc, and its blood suspension was spread evenly. Next, the disc was observed under an USB digital microscope to see whether there was erythrocyte immobilisation on solid media coated with total proteins of gnetum seeds or not. All the erythrocytes from human ABO groups were bound effectively, from strongest to weakest,to paper filters, nitrocellulose membranes, and plastics. The future use of erythrocyte immobilisation on a variety of solid media coated with of gnetum total protein seed may be useful for diagnosing diseases related patological erythrocytes with a specific marker.
{"title":"Erythrocyte Immobilisation with Total Protein Extracted from Gnetum gnemon L. Seeds on Variety of Solid Media","authors":"Hery Haryanto, S. Nurliana, Syariffuddin Syarifuddin","doi":"10.2991/absr.k.210621.060","DOIUrl":"https://doi.org/10.2991/absr.k.210621.060","url":null,"abstract":"The objective of this research was to determine the effectiveness of erythrocyte immobilisation with total protein extracted from gnetum seeds on a variety of solid media, i.e., filter papers, doff plastics, and nitrocellulose membranes. Old gnetum seeds were ground to make powders. The powders were homogenized in ml PBS buffer containing anti-protease PMSF in a cool room. The homogenate was centrifuged in order to separate supernatant from pellet. Solid media were punched with a perforator, so the results were discs of papers, plastics, and nitrocellulose membranes. The solid media perforated discs were then dipped in gnetum total protein supernatants. The dipped discs were air-dried for a while. Human erythrocytes of all ABO groups were washed three times and diluted to suspension of 20% (v/v) in NaCl 0,9% (w/v). A small drop of erythrocyte suspension was put in middle of each solid media disc, and its blood suspension was spread evenly. Next, the disc was observed under an USB digital microscope to see whether there was erythrocyte immobilisation on solid media coated with total proteins of gnetum seeds or not. All the erythrocytes from human ABO groups were bound effectively, from strongest to weakest,to paper filters, nitrocellulose membranes, and plastics. The future use of erythrocyte immobilisation on a variety of solid media coated with of gnetum total protein seed may be useful for diagnosing diseases related patological erythrocytes with a specific marker.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89631022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.015
S. Pudjiono, M. Susanto, Mashudi, D. Setiadi, M. Sulaeman, R. Hartati, Taufik Rahmadi, A. Wibowo
Manglid (Manglietia glauca Bl) is an indigenous tree species in the Indonesian forest whose existence began to be difficult to find. Wood is the part of the plant that can be used. The development of this plant which is the local genetic resources need to be done with the breeding program. In connection with this, the determination of the plant growth is necessary in order to obtain information of manglid ability in producing wood. The purpose of this research was to determine the growth variation of the manglid plants that planted at the Candiroto with an altitude between 457-464 meters above sea level, Candiroto District, Temanggung Regency, Central Java at the age of 42 months. The research method used was a Randomized Complete Block Design with the seedlings propagated from 100 parent trees, separated into 10 blocks, each block consisted of 4 tree plots, and the planting distance was 4 m x 3 m. The characteristics of plant measured were the height of the plant, stem diameter, and life percentage of manglid. The measurement results were analyzed for the variation and tested by employing a Duncan Multiple Range Test. The results showed that the height of plants was ranged between 106-1083 cm with an average of 577 cm. The stem diameter was ranged between 1,3-28,7cm with an average of 9,4 cm. The life percentage of plants was ranged between 17,5-80,0% with an average of 51,3%. The analysis results showed that the parent tree has significant effects on the total height and the stem diameter of plants. There were some variations of height characteristic from 100 parent trees, there were 23 groups, while for the stem diameter characteristic, there were 11 groups.
芒树(Manglietia glauca Bl)是印度尼西亚森林中的一种本土树种，其存在开始变得难以找到。木材是植物中可以利用的部分。这种地方遗传资源的开发需要结合育种计划来完成。与此相关的是，为了获得木材生产能力的信息，测定植物的生长是必要的。本研究的目的是确定种植在中爪哇省Temanggung县Candiroto区海拔457 ~ 464米的Candiroto地区的山核桃植物在42月龄时的生长变化。研究方法采用随机完全区组设计，将100株母树育苗分为10个区组，每个区组由4个树样组成，种植距离为4 m x 3 m。测定植株的性状为株高、茎粗、木质素的活率。对测量结果进行变异分析，并采用Duncan多元极差检验。结果表明:各树种株高在106 ~ 1083 cm之间，平均为577 cm;茎粗在1,3 ~ 28.7 cm之间，平均为9.4 cm。植物的生活率在17.5 ~ 80%之间，平均为51.3%。分析结果表明，母树对植株的总高和茎粗有显著影响。100株亲本的株高性状有23个组，茎粗性状有11个组。
{"title":"The Manglid (Manglietia glauca Bl) Growth Variations at Age of 42 Months in Candiroto Temanggung Central Java","authors":"S. Pudjiono, M. Susanto, Mashudi, D. Setiadi, M. Sulaeman, R. Hartati, Taufik Rahmadi, A. Wibowo","doi":"10.2991/absr.k.210621.015","DOIUrl":"https://doi.org/10.2991/absr.k.210621.015","url":null,"abstract":"Manglid (Manglietia glauca Bl) is an indigenous tree species in the Indonesian forest whose existence began to be difficult to find. Wood is the part of the plant that can be used. The development of this plant which is the local genetic resources need to be done with the breeding program. In connection with this, the determination of the plant growth is necessary in order to obtain information of manglid ability in producing wood. The purpose of this research was to determine the growth variation of the manglid plants that planted at the Candiroto with an altitude between 457-464 meters above sea level, Candiroto District, Temanggung Regency, Central Java at the age of 42 months. The research method used was a Randomized Complete Block Design with the seedlings propagated from 100 parent trees, separated into 10 blocks, each block consisted of 4 tree plots, and the planting distance was 4 m x 3 m. The characteristics of plant measured were the height of the plant, stem diameter, and life percentage of manglid. The measurement results were analyzed for the variation and tested by employing a Duncan Multiple Range Test. The results showed that the height of plants was ranged between 106-1083 cm with an average of 577 cm. The stem diameter was ranged between 1,3-28,7cm with an average of 9,4 cm. The life percentage of plants was ranged between 17,5-80,0% with an average of 51,3%. The analysis results showed that the parent tree has significant effects on the total height and the stem diameter of plants. There were some variations of height characteristic from 100 parent trees, there were 23 groups, while for the stem diameter characteristic, there were 11 groups.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89684622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.080
M. Asril, Y. Lisafitri, A. Niswati, S. R. Dirmawati, R. Rismawati, I. Rini, I. Oktaviani
Phosphorus is the main macronutrient required by plants for growth and development. Phosphorus in the soil in bonds with metals such as Al and Ca, especially in acid soils such as ultisols. This form of phosphate must be mineralized or dissolved using phosphate solubilizing bacteria. The ability to dissolve phosphate also correlates with the production of Indole Acetic Acid (IAA), which can increase plant growth. This study aims to assess the ability of phosphate dissolution and IAA production of three phosphate solubilizing bacteria (EF.NAP 5, EF.NAP 7, and EF.NAP 10) which have been isolated from acid soils, Lampung. The method used was the clear zone test for bacterial isolates, phosphate solubility, and measurement of IAA production during the phosphate solubility process. EF.NAP 7, EF.NAP 5 and EF.NAP 10 isolates had phosphate dissolution indexes of 0.986 (6th day), 0.828 (7th day) and 0.789 (3rd day), respectively. EF.NAP 7 isolate (392.87 mg/L) was able to dissolve phosphate better than EF.NAP 10 (311 mg/L) and EF.NAP 5 (213.37 mg/L). The pH conditions on the highest phosphate solubility were EF.NAP 7 (4.94), EF.NAP 10 (4.19) and EF.NAP 5 (6.01), respectively. The three isolates of phosphate solubilizing bacteria were able to produce IAA during the phosphate dissolving process in the Liquid Pikovskaya medium without the addition of Tryptophan with IAA concentrations of EF.NAP 5 (12.5 mg/L), EF.NAP 7 (8.0 mg/L) and EF.NAP 10 (8.5 mg/L), respectively.
{"title":"Assessment of Phosphate Solubilization and Indole Acetic Acid Production of Phosphate Solubilizing Bacteria Isolated from Acid Soils, Lampung, Indonesia","authors":"M. Asril, Y. Lisafitri, A. Niswati, S. R. Dirmawati, R. Rismawati, I. Rini, I. Oktaviani","doi":"10.2991/absr.k.210621.080","DOIUrl":"https://doi.org/10.2991/absr.k.210621.080","url":null,"abstract":"Phosphorus is the main macronutrient required by plants for growth and development. Phosphorus in the soil in bonds with metals such as Al and Ca, especially in acid soils such as ultisols. This form of phosphate must be mineralized or dissolved using phosphate solubilizing bacteria. The ability to dissolve phosphate also correlates with the production of Indole Acetic Acid (IAA), which can increase plant growth. This study aims to assess the ability of phosphate dissolution and IAA production of three phosphate solubilizing bacteria (EF.NAP 5, EF.NAP 7, and EF.NAP 10) which have been isolated from acid soils, Lampung. The method used was the clear zone test for bacterial isolates, phosphate solubility, and measurement of IAA production during the phosphate solubility process. EF.NAP 7, EF.NAP 5 and EF.NAP 10 isolates had phosphate dissolution indexes of 0.986 (6th day), 0.828 (7th day) and 0.789 (3rd day), respectively. EF.NAP 7 isolate (392.87 mg/L) was able to dissolve phosphate better than EF.NAP 10 (311 mg/L) and EF.NAP 5 (213.37 mg/L). The pH conditions on the highest phosphate solubility were EF.NAP 7 (4.94), EF.NAP 10 (4.19) and EF.NAP 5 (6.01), respectively. The three isolates of phosphate solubilizing bacteria were able to produce IAA during the phosphate dissolving process in the Liquid Pikovskaya medium without the addition of Tryptophan with IAA concentrations of EF.NAP 5 (12.5 mg/L), EF.NAP 7 (8.0 mg/L) and EF.NAP 10 (8.5 mg/L), respectively.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79622216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.059
Delfiani Anggias Putri, R. H. Wibowo, Sipriyadi, W. Darwis, C. Muslim, S. Yudha, Hizkia Puspa Pertiwi
Sponges including multicellular animals, do not have actual tissues and organs, and are the simplest animals structurally. Sponges are also a group of marine life that is symbiotic with the microbial community. Microbes that have formed a symbiotic relationship with a sponge will produce secondary metabolites which also protect their host from pathogens. The sponge used in this study is the Aplysina sp. sponge collected from Enggano island, Bengkulu Province. Aplysina sp sponge is known to contain metabolites with antimicrobial, repellent properties, fighting predators, cytotoxic activity of breast cancer cells, has an antibiotic and anti-inflammatory effect on gram-positive bacteria. This study aims to identify of potential isolates associated with Aplysina sp. sponge collected from Enggano island. Isolation of bacteria from Aplysina sp. sponge using Sea Water Complete (SWC) media. The isolates were screened by antagonistic test, morphological characters, Gram-staining, biochemical test and molecular identification. Based on the antagonistic test, APD7 isolate could inhibit Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus in vitro. APD7 isolate was identified molecularly using of 16S rRNA genes analysis and it genetically close with Staphylococcus sciuri.
{"title":"Antibacterial Activities of Staphylococcus sciuri APD7 Isolate Sponge-Associated Aplysina sp. Against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus","authors":"Delfiani Anggias Putri, R. H. Wibowo, Sipriyadi, W. Darwis, C. Muslim, S. Yudha, Hizkia Puspa Pertiwi","doi":"10.2991/absr.k.210621.059","DOIUrl":"https://doi.org/10.2991/absr.k.210621.059","url":null,"abstract":"Sponges including multicellular animals, do not have actual tissues and organs, and are the simplest animals structurally. Sponges are also a group of marine life that is symbiotic with the microbial community. Microbes that have formed a symbiotic relationship with a sponge will produce secondary metabolites which also protect their host from pathogens. The sponge used in this study is the Aplysina sp. sponge collected from Enggano island, Bengkulu Province. Aplysina sp sponge is known to contain metabolites with antimicrobial, repellent properties, fighting predators, cytotoxic activity of breast cancer cells, has an antibiotic and anti-inflammatory effect on gram-positive bacteria. This study aims to identify of potential isolates associated with Aplysina sp. sponge collected from Enggano island. Isolation of bacteria from Aplysina sp. sponge using Sea Water Complete (SWC) media. The isolates were screened by antagonistic test, morphological characters, Gram-staining, biochemical test and molecular identification. Based on the antagonistic test, APD7 isolate could inhibit Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus in vitro. APD7 isolate was identified molecularly using of 16S rRNA genes analysis and it genetically close with Staphylococcus sciuri.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82526368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.044
Fitmawati, A. Saputra, S. N. Kholifah, E. Resida, R. Roza, Emrizal
The development of medicine requires preclinical testing of experimental animals. One of the potential plants to be developed as a new medicinal source is Sumatran wild mangoes (Mangifera spp.) which are known to have immunomodulatory activity and higher antioxidant value than cultivated Mangifera. This study was aimed to test the sub-chronic toxicity of the extract of 6 wild mango species on white rats’ kidney function. This study used 21 treatments and 3 replications, consisted of 3 control treatments (control 0, with distilled water, positive control (stimuno) and negative control (CMC-Na 1%), and 6 types of Mangifera spp. (Mangifera sp.2 (Hutan), M. sumatrana, M. foetida (var. Batu, Limus, and Manis), and M. laurina) with 3 different dosages. Observations were made macroscopically (morphology) and histology through the glomerulus image to see the percentage of damage. The percentage of glomerulus damage was analyzed by ANOVA and Duncan's test. Based on the results showed that the kidney morphology looked like a pea with brownish-red color and the capsule looked flat (healthy) after being treated. Histologically, the administration of wild mango extract did not cause damage to the rats' kidneys, which was indicated by the glomerulus which was still covered by the bowman capsule and the boundary between the pars visceral epithelium and pars parietal epithelium was clearly visible.
药物的开发需要实验动物的临床前试验。其中一个潜在的药用植物是苏门答腊野生芒果(Mangifera spp.)，已知其具有免疫调节活性和比栽培芒果更高的抗氧化价值。研究6种野生芒果提取物对大鼠肾脏功能的亚慢性毒性作用。本研究共设21个处理，3个重复，包括3个对照处理(对照0，蒸馏水处理，阳性对照(刺激)和阴性对照(cc - na 1%))， 6种芒果属植物(芒果属2号(胡檀)、苏门答腊、竹竹(巴图、Limus和Manis品种)和月桂属)3种不同剂量。通过肾小球图像进行宏观(形态学)和组织学观察，观察损伤百分率。采用方差分析和Duncan检验分析肾小球损伤百分率。结果显示，处理后肾形态呈豌豆状，呈棕红色，肾囊呈扁平状(健康)。组织学上，野生芒果提取物未对大鼠肾脏造成损伤，可见肾小球仍被鲍曼囊覆盖，肾部内脏上皮和肾部壁上皮的边界清晰可见。
{"title":"Morphological and Histological Study of White Rats (Rattus norvegicus) Kidney Following the Consumption of Sumatran Wild Mango Extract (Mangifera spp.)","authors":"Fitmawati, A. Saputra, S. N. Kholifah, E. Resida, R. Roza, Emrizal","doi":"10.2991/absr.k.210621.044","DOIUrl":"https://doi.org/10.2991/absr.k.210621.044","url":null,"abstract":"The development of medicine requires preclinical testing of experimental animals. One of the potential plants to be developed as a new medicinal source is Sumatran wild mangoes (Mangifera spp.) which are known to have immunomodulatory activity and higher antioxidant value than cultivated Mangifera. This study was aimed to test the sub-chronic toxicity of the extract of 6 wild mango species on white rats’ kidney function. This study used 21 treatments and 3 replications, consisted of 3 control treatments (control 0, with distilled water, positive control (stimuno) and negative control (CMC-Na 1%), and 6 types of Mangifera spp. (Mangifera sp.2 (Hutan), M. sumatrana, M. foetida (var. Batu, Limus, and Manis), and M. laurina) with 3 different dosages. Observations were made macroscopically (morphology) and histology through the glomerulus image to see the percentage of damage. The percentage of glomerulus damage was analyzed by ANOVA and Duncan's test. Based on the results showed that the kidney morphology looked like a pea with brownish-red color and the capsule looked flat (healthy) after being treated. Histologically, the administration of wild mango extract did not cause damage to the rats' kidneys, which was indicated by the glomerulus which was still covered by the bowman capsule and the boundary between the pars visceral epithelium and pars parietal epithelium was clearly visible.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75228847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.027
M. A. Fadila, N. S. Ariyanti, E. B. Walujo
Local wisdom is a legacy that must be preserved and documented. Using plants as traditional medicine is examples of local wisdom. One of the tribes in Indonesia that called Serawai tribe, are still using the surrounding plants as traditional medicine. Documenting local knowledge in making a drug from medicinal plants is part of the implementation of Nagoya Protocol that has been ratified by Indonesia Government in UU RI number 11 2013. Some medicinal plants are used by mixing more than one species to make it a medicinal herb. These medicinal ingredients are often used orally. Therefore, this study aims to document oral formulations based on medicinal plants used by the Serawai tribe in Seluma, Bengkulu. The research was conducted using the exploring method based on informations obtained through open interviews with several key informants. The explorations were carried out in four districts in Seluma Regency, Bengkulu. This research found at least 28 species of medicinal plants that used as ingredients of traditional medicine which are applied orally. These types of medicinal plants contained of alkaloids, phenol and terpenoids.
{"title":"Oral Herbs Based on Serawai Seluma Medicinal Plants","authors":"M. A. Fadila, N. S. Ariyanti, E. B. Walujo","doi":"10.2991/absr.k.210621.027","DOIUrl":"https://doi.org/10.2991/absr.k.210621.027","url":null,"abstract":"Local wisdom is a legacy that must be preserved and documented. Using plants as traditional medicine is examples of local wisdom. One of the tribes in Indonesia that called Serawai tribe, are still using the surrounding plants as traditional medicine. Documenting local knowledge in making a drug from medicinal plants is part of the implementation of Nagoya Protocol that has been ratified by Indonesia Government in UU RI number 11 2013. Some medicinal plants are used by mixing more than one species to make it a medicinal herb. These medicinal ingredients are often used orally. Therefore, this study aims to document oral formulations based on medicinal plants used by the Serawai tribe in Seluma, Bengkulu. The research was conducted using the exploring method based on informations obtained through open interviews with several key informants. The explorations were carried out in four districts in Seluma Regency, Bengkulu. This research found at least 28 species of medicinal plants that used as ingredients of traditional medicine which are applied orally. These types of medicinal plants contained of alkaloids, phenol and terpenoids.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75814814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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