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Vaginal Smear and Neutrophil Count as an Alternative Method for Estrous Phase in Female Tiger (Panthera tigris sumatrae, Pocock, 1929) 阴道涂片和中性粒细胞计数作为雌性老虎发情期的替代方法(Panthera tigris sumatrae, Pocock, 1929)
Genoveva Kiranaputri, L. Sjahfirdi, L. Tumbelaka
Detection of the estrous cycle is essential for basic reproductive aspects in all animals. Both natural mating and assisted reproductive techniques require estrous detection for breeding programs which will assist to maintain the tiger population in captivity. This research aims to explore alternative methods for tiger conservation in the remote area. Vaginal smear and neutrophil count were used to determine the phase of estrous cycle, and it easily applied on the field. Vaginal smears and blood smears were collected twice within interval 6 months n a female tiger at Tambling Wildlife Nature Conservation Rescue Centre. While tiger was restrained mechanically, blood samples were collected from the coccygeal vein and the vaginal smears were collected intravaginal. All procedure followed the animal welfare protocol. Vaginal smears were stained by 10% Giemsa. Basal/parabasal, intermediate, and superficial/cornified cells were identified to determine the reproductive periods under the microscope. Blood smears were stained by Giemsa-Wright. Neutrophils were counted within 200 white blood cells count under the microscope. The result showed that female tiger was in the follicular phase and neutrophil count was 78 cells at the 1 sample collection. The 2 sample collection resulted in luteal phase with 55 neutrophils count. This research suggests vaginal smear and neutrophil count can be used as an alternative method of tiger estrous phase detection. In addition, these methods have economical and practical value.
探测发情周期对于所有动物的基本生殖方面都是必不可少的。自然交配和辅助生殖技术都需要在繁殖计划中进行动情检测,这将有助于维持圈养老虎的数量。本研究旨在探索偏远地区老虎保护的替代方法。用阴道涂片和中性粒细胞计数来判断发情周期,在野外应用方便。在坦布林野生动物自然保育救援中心对一只雌性老虎进行了两次阴道涂片和血液涂片检查,每隔6个月进行一次。在对虎进行机械约束的同时,采集尾骨静脉血样和阴道内涂片。所有程序均遵循动物福利规程。阴道涂片10%吉姆氏染色。鉴定基底/准基底、中间和表面/角化细胞,以确定显微镜下的生殖周期。血涂片用吉姆萨-赖特染色法染色。显微镜下计数200个白细胞内的中性粒细胞。结果表明,雌虎处于卵泡期,1次采集时中性粒细胞计数为78个。2例黄体期中性粒细胞计数55。本研究提示阴道涂片和中性粒细胞计数可作为虎发情期检测的替代方法。此外,这些方法具有经济实用价值。
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引用次数: 0
Ethnobotanical Study of Pitcher Plant (Nepenthes sp.): A Medicine for Diarrhea and Gastroenteritis of Dayak Sampit People 猪笼草(Nepenthes sp.)的民族植物学研究:一种治疗达雅Sampit人腹泻和胃肠炎的药物
Dedy Idamansyah, Ayatusa’adah
The Dayak Sampit people believe that pitcher plant is a medicine for diarrhea and gastroenteritis. This study aims to depict the ethnobotanical study of pitcher plants in the tradition of Dayak Sampit people of Singsingan Hamlet, Tehang Village, Parenggean Sub-district, Kotawaringin Timur Regency. The research method is descriptive qualitative with data collection technique of interview with key informants. The key informants include gatherer/batra at Singsingan Hamlet Tehang Village, pitcher plant users, and the village elders. The informants are selected using purposive sampling method. The research results suggest that Dayak Sampit people utilize and believe that pitcher plant roots could cure diarrhea and gastroenteritis. The Dayak Sampit people call the plant as Tabeko due to its morphological form that resembles a teapot. The plant utilization as a medicine for diarrhea and gastroenteritis by Dayak people of Singsingan hamlet is observed from the root taking and potion making stages. The plant has phytochemicals, plumbagin, 2-methylnaphthazarin, octadecyl caffeate, isoshinanolone, and droserone that indicate reaction as anti-malaria. The results suggest that pitcher plants have a potential as a medicine for diarrhea and gastroenteritis.
达亚克Sampit人相信猪笼草是治疗腹泻和肠胃炎的药。本研究旨在描述铁木尔摄政王高塔瓦林县帕林吉安街道特hang村Singsingan村达亚克Sampit人传统的猪笼草民族植物学研究。研究方法采用描述性定性方法,采用对关键线人进行访谈的数据收集技术。主要举报人包括Singsingan Hamlet Tehang村的采集者/batra、猪笼草使用者和村里的长老。采用有目的的抽样方法选择举报人。研究结果表明,达雅Sampit人利用并相信猪笼草的根可以治疗腹泻和肠胃炎。由于其形态与茶壶相似,达雅Sampit人将其称为Tabeko。从取根和制药两个阶段考察了新新安村达雅克人对腹泻和肠胃炎的药物利用。该植物含有植物化学物质,白桦素,2-甲基萘萨林,十八烷基咖啡酸酯,异草胺酮和德罗塞隆,表明对抗疟疾有反应。结果表明猪笼草有可能作为治疗腹泻和胃肠炎的药物。
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引用次数: 0
Antibacterial Activities of Endophytic Bacteria Isolate AKEBG28 from Yellow Root Plant (Arcangelisia flava (L.) Merr) Against Escherichia coli and Staphylococcus aureus 黄根植物(Arcangelisia flava)内生细菌AKEBG28的抑菌活性抗大肠杆菌和金黄色葡萄球菌
Sipriyadi, R. H. Wibowo, W. Darwis, Resli Siboro, S. Yudha
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引用次数: 0
Analysis of Water Quality from Several Rivers as Habitat of Mungkus Fish (Sicyopterus sp.) in Bengkulu Province 明古鲁省几条河流蒙古斯鱼栖息地水质分析
A. Putra, D. Lestari, Sipriyadi
Mungkus Fish (Sicyopterus sp.) is Gobiid fish inhabiting fast flowing and good quality river in Bengkulu Province. Today, it is rare to find Mungkus fish in the river. One of the extinction threats is the decline of water quality. This research was aimed to measure abiotic factor like chemical and physical factor in several rivers in Bengkulu Province where can be found Mungkus fish. Sampling was done in 8 rivers from 5 regencies. Chemical factors tested in this research were oil, Ammonia, Fe, BOD, nitrate, nitrite, phosphate, and detergent. Physical factors involved temperature, pH, DO, TSS, and TDS. The result of chemical factor test showed that chemical compounds of water from 8 rivers contained Ammonia (0.36-0.43 ppm), oil (0-40 ppm) nitrate (0.0160.79 ppm), nitrite (0.0017-0.0038 ppm), Fe (0.051-7.24 ppm), phosphate (0-0.77 ppm), and detergent (0.260.479 ppm). Data of physical test showed that temperature range was 25C (at NPS) to 28C (at LKN). Conductivity range was 4.31 μs (at LKN) to 59.01 μs (at MRS), pH 7.99-8.7, TSS 13.89 ppm to 34.23 ppm, TDS 0.022 ppt to 0.039 ppt and DO concentration reached 6,32 (LKN) to 7,99 (ARM). The result can be concluded based on abiotic factor that 8 rivers are classified as good quality rivers.
孟古鱼(Sicyopterus sp.)是一种生活在明古鲁省湍急水质良好的河流中的Gobiid鱼。如今,在这条河里很难找到蒙古斯鱼。其中一个灭绝的威胁是水质的下降。本研究旨在测量明古鲁省几条河流的化学和物理因素等非生物因素,这些河流可以发现蒙古斯鱼。在5个县的8条河流中进行了采样。本研究测试的化学因素有油、氨、铁、BOD、硝酸盐、亚硝酸盐、磷酸盐和洗涤剂。物理因素包括温度、pH、DO、TSS和TDS。化学因子测试结果显示,8条河流的水的化学成分分别为氨(0.36 ~ 0.43 ppm)、油(0 ~ 40 ppm)、硝酸盐(0.0160.79 ppm)、亚硝酸盐(0.0017 ~ 0.0038 ppm)、铁(0.051 ~ 7.24 ppm)、磷酸盐(0 ~ 0.77 ppm)、洗涤剂(0.26 ~ 0.479 ppm)。物理试验数据表明,温度范围为25C (NPS)至28C (LKN)。电导率范围为4.31 μs (LKN) ~ 59.01 μs (MRS), pH为7.99 ~ 8.7,TSS为13.89 ppm ~ 34.23 ppm, TDS为0.022 ~ 0.039 ppt, DO浓度为6.32 (LKN) ~ 7.99 (ARM)。根据非生物因子分析结果,8条河流为优良河。
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引用次数: 0
The Potency of Bacillus siamensis LDR as Biocontrol Agent Against Fungal Phytopathogen 暹罗芽孢杆菌LDR对真菌植物病原菌的防效研究
I. Santoso, Q. G. Fadhilah, A. E. Maryanto, Yasman
Bacillus spp. was recognized as biocontrol agent because could produce antifungal towards many phytopathogenic fungi. In relation to study the potency of B. siamensis LDR as biocontrol, antagonistic activity of the bacilli was evaluated against Fusarium sp., Ganoderma sp., and Chaetomium globosum InaCC F228. The antagonist assay was carried out by dual culture method using streak and pour plate technique. Result of antagonistic activity assay with streak technique showed that growth inhibition rate of Fusarium sp., Ganoderma sp., and Chaetomium globosum InaCC F228 were 36.83%, 55.20%, and 33.78%, respectively. Antagonist assay using pour plate technique showed that percentage growth of inhibition for Fusarium sp. (93.96%), Ganoderma sp. (100%), and Chaetomium globosum InaCC F228 (76.64%) were higher than the results from streak method. Furthermore, results from antibiosis assay showed that growth inhibition rate of Fusarium sp., Ganoderma sp., and Chaetomium globosum InaCC F228 were 10.85%, 73.31%, and 30.32% respectively.
芽孢杆菌对许多植物病原真菌具有抗真菌作用,是公认的生物防治剂。为了研究结核分枝杆菌的生物防治效果,测定了结核分枝杆菌对镰刀菌、灵芝菌和球毛菌InaCC F228的拮抗活性。拮抗试验采用条纹法和平板法双重培养。条纹法测定病原菌对镰刀菌、灵芝菌和球毛菌InaCC F228的抑制率分别为36.83%、55.20%和33.78%。对镰刀菌(93.96%)、灵芝(100%)、毛毛菌InaCC F228的抑制率(76.64%)均高于条纹法。抗菌试验结果表明,菌株对镰刀菌、灵芝菌和毛毛菌InaCC F228的生长抑制率分别为10.85%、73.31%和30.32%。
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引用次数: 2
Population and Habitat Use of the Critically Endangered Grey-Backed Myna (Acridotheres tricolor) in the One of Easternmost Protected Area in Java 爪哇最东端保护区之一极度濒危灰背八哥的种群数量及生境利用
B. G. Anugra, N. Winarni, D. H. Pradana, Tom Squires
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引用次数: 0
Feeding Preference and Daily Activities of Deer (Axis axis Erxl. 1777) in the Captivity of Wari Park, South Sumatra Province 南苏门答腊省Wari公园圈养鹿(Axis Axis Erxl. 1777)的摄食偏好和日常活动
Rizwar, Kamilah, Darmi, Syarifuddin, Fenti Ade Nelda, Debi Oktarina
Deer (Axis axis) are often cultivated in captivity of city park because they can be used to add population in natural habitats, beautifying city parks and as regional tourism objects. The success factors in deer breeding depend on the providing of the type of feed they like with a sufficient quantity of feed to produce a healthy deer generation. Status of a deer health is also determined by the understanding of the manager about the dynamics of their daily activities in captivity. This study aims to determine the preference of spotted deer on several types of feed given and their daily activities in captivity. The types of feed given were city vegetable wastes such as sweet potatoes (Ipomoea batatas), long beans (Vigna sinensis), bananas (Musa paradisiaca) and others in the form of grasses, rice bran. The study was conducted from May to July 2015 in Wari Park Captivity, Lahat City. The study of feed preferences used the Neu method. Nine individuals (3 adult males, 3 adult females and 3 juveniles) were given five kinds of food. The results showed that spotted deers more preferred bananas and sweet potatoes with preference index w> 1 than rice bran, long beans and grasses (w <1). The higher daily activities of Axis axis in captivity were resting (42.92%), moving or locomotion (26.30%), and eating (22.89%), while the lower activities were social activity (1,04%), faecal disposal (1.09%), Agonistic (2.37%) and grooming
鹿(中轴线)通常是城市公园圈养的,因为它们可以用来增加自然栖息地的人口,美化城市公园和作为区域旅游对象。鹿繁殖的成功因素取决于提供它们喜欢的饲料类型和足够数量的饲料来生产健康的鹿一代。鹿的健康状况也取决于饲养员对它们在圈养环境中日常活动动态的了解。本研究旨在确定斑鹿对几种饲料的偏好以及它们在圈养环境下的日常活动。饲料的种类是城市蔬菜废料,如甘薯(Ipomoea batatas),长豆(Vigna sinensis),香蕉(Musa paradisiaca)和其他形式的草,米糠。该研究于2015年5月至7月在拉哈特市Wari公园圈养区进行。饲料偏好的研究采用了Neu方法。9只个体(雄性3只,雌性3只,幼体3只)分别被喂食5种食物。结果表明:斑鹿对香蕉和红薯的偏好指数w> 1,优于米糠、长豆和禾草(w <1);圈养中轴线的日常活动以休息(42.92%)、移动(26.30%)和进食(22.89%)居多,社交(104%)、排便(1.09%)、交配(2.37%)和梳理毛发居多
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引用次数: 0
Isolation of Seed-borne Fungal Endophytes on Sengon (Falcataria Moluccana) 沙魔芋种子内生真菌的分离
Y. Istikorini, N. F. Haneda, U. J. Siregar, D. Kusuma
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引用次数: 1
Early Detection of Body Temperature with Infrared Technology for Prevention of Endemic Outbreaks of Primary School Age Infectious Diseases 利用红外技术早期检测体温预防小学年龄传染病地方性暴发
S. Rahayu, D. Priatna, Zulpi Zulkarnaen
Initial detection of body temperature with infrared technology is currently only used for diagnosis of an infectious disease, but has not been used as a parameter to prevent disease transmission, resulting in an increase in the rate of disease transmission. The use of a device to measure the increase in body temperature of school pupils, namely the mercury thermometer, requires physical contact or contact with the body between the gauge, the measured school student and the measuring instrument, and requires a long time, so that it will interfere with learning activities at school. Physical contact when using a mercury thermometer can potentially be a medium for disease transmission. This study aims to analyze the effect of students whose body temperature is above normal who do not attend school on preventing the transmission of infectious disease and the effectiveness and accuracy of an infrared thermometer in comparison with a digital thermometer. The research design was a cross sectional or crosssectional study, where measurements were only carried out once at a time for a population in the school. The sample to be given treatment came from grade V students with a total of 40 students from 4 classes totaling 146 students. From data testing using SPSS, it was found that the use of an infrared thermometer was effective almost 100% in preventing outbreaks of transmission of infectious diseases in elementary school-aged students. The infrared thermometer is easy to operate and gives students a sense of security and comfort. So that early detection of body temperature with infrared technology for the prevention of endemic outbreaks of infectious diseases in elementary school children is highly recommended.
利用红外技术对体温进行初步检测,目前仅用于一种传染病的诊断,尚未作为预防疾病传播的参数,导致疾病传播率上升。使用一种测量小学生体温升高的装置,即水银体温计,量具、被测小学生与测量仪器之间需要有身体接触或身体接触,且需要较长时间,这样会干扰学校的学习活动。使用水银温度计时的身体接触可能成为疾病传播的媒介。本研究旨在分析体温高于正常而不上学的学生对预防传染病传播的影响,以及红外体温计与数字体温计的有效性和准确性。研究设计是一个横断面或横断面研究,其中测量只进行一次在学校的人口。接受治疗的样本来自五年级学生,共40名学生,来自4个班级,共计146名学生。利用SPSS软件对数据进行检验,发现红外测温仪在小学生中预防传染病传播的有效性几乎为100%。红外测温仪操作简单,给学生安全感和舒适感。因此,强烈建议利用红外技术对小学生进行体温早期检测,以预防地方性传染病的暴发。
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引用次数: 0
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Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)
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