Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.011
Novia Duya, Risma Hurhidayah, Jarulis
Fish is an aquatic animal that has a high diversity. Baai Island Fish Auction Place (TPI) is a fish landing place used as the largest fishing center in the city of Bengkulu. The purpose of this study was to determine the diversity and morphometric characteristics of the different types of Chondrichthyes fish caught by fishermen in Baai Island, Bengkulu City. Sampling was conducted from October-November 2019 with a frequency of once a week using the direct survey method. The fish samples obtained were measured and observed for their morphology and preserved using 70% alcohol. Measurement data were analyzed using software Minitab 16 and morphological observation data were analyzed descriptively. The results showed that there were four orders, six families, seven genera and 10 species. The best PC combination to separate genera of sharks and rays is the combination of PC3 and PC4. The best combination of characters that can separate shark species (genus Carcharhinus) is a combination of PL (pradorsal length) with HL (head length), DFH (dorsal fin height) with HL (head length), DFH (dorsal fin height) with HH (head height), and HL (head length) with HH (head height). The best combination of characters that can separate stingrays (genus Himantura) is the combination of TL (total length) with HL (head length), PFL (pectoral fin length) with AFL (Abdominal fin length), and TSL (tail shaft length) with DL (disc length). In the observation of shark morphology, the most body shape found is the torpedo shape (fusiform) and the most common type of tail fin is the epicercal tail type. Whereas in the observation of the morphology of stingrays, the most body shape found was depressiform and the most common type of tail fin was long tail fin like a whip.
{"title":"Biometric Diversity of Chondrichthyes Caught by Bengkulu City Fishermen Observed at the Fish Auction Site (TPI) Pulau Baai Bengkulu","authors":"Novia Duya, Risma Hurhidayah, Jarulis","doi":"10.2991/absr.k.210621.011","DOIUrl":"https://doi.org/10.2991/absr.k.210621.011","url":null,"abstract":"Fish is an aquatic animal that has a high diversity. Baai Island Fish Auction Place (TPI) is a fish landing place used as the largest fishing center in the city of Bengkulu. The purpose of this study was to determine the diversity and morphometric characteristics of the different types of Chondrichthyes fish caught by fishermen in Baai Island, Bengkulu City. Sampling was conducted from October-November 2019 with a frequency of once a week using the direct survey method. The fish samples obtained were measured and observed for their morphology and preserved using 70% alcohol. Measurement data were analyzed using software Minitab 16 and morphological observation data were analyzed descriptively. The results showed that there were four orders, six families, seven genera and 10 species. The best PC combination to separate genera of sharks and rays is the combination of PC3 and PC4. The best combination of characters that can separate shark species (genus Carcharhinus) is a combination of PL (pradorsal length) with HL (head length), DFH (dorsal fin height) with HL (head length), DFH (dorsal fin height) with HH (head height), and HL (head length) with HH (head height). The best combination of characters that can separate stingrays (genus Himantura) is the combination of TL (total length) with HL (head length), PFL (pectoral fin length) with AFL (Abdominal fin length), and TSL (tail shaft length) with DL (disc length). In the observation of shark morphology, the most body shape found is the torpedo shape (fusiform) and the most common type of tail fin is the epicercal tail type. Whereas in the observation of the morphology of stingrays, the most body shape found was depressiform and the most common type of tail fin was long tail fin like a whip.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90152009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.070
Tiara Egga Agustina, W. Handayani, C. Imawan
Research on the biological synthesis of silver nanoparticles (AgNPs) using plant extracts as reducing agents is growing rapidly because they are eco-friendly and cost-effective. Diospyros maritima Blume. is one of the plant species that had potential in the biosynthesis of AgNPs. In this study, biosynthesis of AgNPs was carried out by varying volume ratio of the extract and AgNO3 (5.0: 1; 2.0: 1; 0.5: 1; 0.2: 1; 0.1: 1) in reaction time (25 minutes, 2, 3, 24, 48, and 96 hours). The phytochemical screening results showed leaves extract of D. maritima contained phenols, flavonoids, saponins, and alkaloids, which may act as a reducing agent of Ag to Ag0. The biosynthetic solution's color was changed from brown to dark brown or black with the increasing reaction time. The UV-Vis spectra confirmed the formation of silver nanoparticles. The UV-Vis spectrum results showed the highest absorption peaks at 400 nm in solutions with a volume ratio of 0.5 :1, which showed the localized surface plasmon resonance (LSPR) characteristics of silver nanoparticles. From the UV-Vis spectrum can be analized that there are more than one peak of absorption at 350–500 nm in solutions with a volume ratio of 2.0:1. We carried out deconvolution of UV-Vis spectrum to separate each absorption peak. The formation of more than one absorption peak in the UV-Vis spectrum indicates a certain shape and silver nanoparticles size.
{"title":"The UV-VIS Spectrum Analysis From Silver Nanoparticles Synthesized Using Diospyros maritima Blume. Leaves Extract","authors":"Tiara Egga Agustina, W. Handayani, C. Imawan","doi":"10.2991/absr.k.210621.070","DOIUrl":"https://doi.org/10.2991/absr.k.210621.070","url":null,"abstract":"Research on the biological synthesis of silver nanoparticles (AgNPs) using plant extracts as reducing agents is growing rapidly because they are eco-friendly and cost-effective. Diospyros maritima Blume. is one of the plant species that had potential in the biosynthesis of AgNPs. In this study, biosynthesis of AgNPs was carried out by varying volume ratio of the extract and AgNO3 (5.0: 1; 2.0: 1; 0.5: 1; 0.2: 1; 0.1: 1) in reaction time (25 minutes, 2, 3, 24, 48, and 96 hours). The phytochemical screening results showed leaves extract of D. maritima contained phenols, flavonoids, saponins, and alkaloids, which may act as a reducing agent of Ag to Ag0. The biosynthetic solution's color was changed from brown to dark brown or black with the increasing reaction time. The UV-Vis spectra confirmed the formation of silver nanoparticles. The UV-Vis spectrum results showed the highest absorption peaks at 400 nm in solutions with a volume ratio of 0.5 :1, which showed the localized surface plasmon resonance (LSPR) characteristics of silver nanoparticles. From the UV-Vis spectrum can be analized that there are more than one peak of absorption at 350–500 nm in solutions with a volume ratio of 2.0:1. We carried out deconvolution of UV-Vis spectrum to separate each absorption peak. The formation of more than one absorption peak in the UV-Vis spectrum indicates a certain shape and silver nanoparticles size.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87775594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.069
Surya, A. Y. Fadwiwati, M. Dagong
{"title":"The Relation of IGF-1 Genotype with Weight in Etawah Grade Goats at Polewali Mandar Regency","authors":"Surya, A. Y. Fadwiwati, M. Dagong","doi":"10.2991/absr.k.210621.069","DOIUrl":"https://doi.org/10.2991/absr.k.210621.069","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85433528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.053
Santi Nurul Kamilah, Mahesha Rama, Jarulis
Bats are the true flying mammals in the world. Morphological identification based upon morphometric characteristics will needed to determine the type of bat species, particularly the closely related species with the high level of morphological similarity. This study was aimed to determine the distinguishing characteristics between bat species found in the campus area of the University of Bengkulu. Bats were caught using mist net. The data of body morphometric measurement of each bat was analyzed using the software of MINITAB 18. As a result, there were four species, namely Cynopterus branchyotis, Cynopterus sphinx of the suborder Megachiroptera, Macroglossus sobrinus, and Rhinolophus stheno of the suborder Microchiroptera. The main distinguishing morphometric characteristics between species within genus were forearm length and tibia length. Based on the morphometric characteristic of all bat species found in this study, Cynopterus branchyotis and Cynopterus sphinx was found to be the most closely related with a Similarity Index of 77.99%, while the Similarity Index between the species and Rhinolophus stheno was 28.87%. Moreover, the lowest Similarity Index was obtained between the species and Macroglossus sobrinus of only 0.01%.
{"title":"Morphometric Analysis of Bats (Chiroptera) in the Campus Area of the University of Bengkulu, Using Principal Component Analysis","authors":"Santi Nurul Kamilah, Mahesha Rama, Jarulis","doi":"10.2991/absr.k.210621.053","DOIUrl":"https://doi.org/10.2991/absr.k.210621.053","url":null,"abstract":"Bats are the true flying mammals in the world. Morphological identification based upon morphometric characteristics will needed to determine the type of bat species, particularly the closely related species with the high level of morphological similarity. This study was aimed to determine the distinguishing characteristics between bat species found in the campus area of the University of Bengkulu. Bats were caught using mist net. The data of body morphometric measurement of each bat was analyzed using the software of MINITAB 18. As a result, there were four species, namely Cynopterus branchyotis, Cynopterus sphinx of the suborder Megachiroptera, Macroglossus sobrinus, and Rhinolophus stheno of the suborder Microchiroptera. The main distinguishing morphometric characteristics between species within genus were forearm length and tibia length. Based on the morphometric characteristic of all bat species found in this study, Cynopterus branchyotis and Cynopterus sphinx was found to be the most closely related with a Similarity Index of 77.99%, while the Similarity Index between the species and Rhinolophus stheno was 28.87%. Moreover, the lowest Similarity Index was obtained between the species and Macroglossus sobrinus of only 0.01%.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75862086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.072
A. Parhusip, Trifena A. Wijaya, Wahyu Irawati
{"title":"Reduction of Heavy Metals in Squid, Green Mussels, White Shrimp Using Combination of Saccharomyces cerevisiae and Candida albicans","authors":"A. Parhusip, Trifena A. Wijaya, Wahyu Irawati","doi":"10.2991/absr.k.210621.072","DOIUrl":"https://doi.org/10.2991/absr.k.210621.072","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90888341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.087
Stella Reformanda, Sipriyadi, W. Darwis, R. H. Wibowo, R. Supriati, Resli Siboro
Endophytic bacteria are bacteria that live in plant tissue without causing disease in their host. In general, endophytic bacteria enter through stomata or wounds in plants by producing cellulase enzymes to degrade cellulose in plant cell walls that contain cellulose, one of which is yellow root (Arcangelisia flava (L.) Merr). The purpose of this study was to isolate endophytic bacteria from yellow roots from Enggano Island, Bengkulu Province, and to identify morphologically, Gram staining and biochemical tests as well as testing the potential of endophytic bacteria in producing cellulase enzymes. Isolation was carried out by the patch method with surface sterilization, using 70% alcohol and 5.25% sodium hypochlorite and then put on NA media that had been treated with Nystatin. The isolates obtained were then tested for their ability to produce cellulase enzymes by scratching them onto CMC media, then measuring the forming of clear zone. The results showed that from 29 isolates, 26 of them were able to degrade cellulose. AKEBG26 and AKEBG25 isolates had a higher ability to hydrolyze cellulose with cellolulitic potential index (IP) were about ±2.90 and ±1.51. Identification based on gram staining and biochemical tests of 8 endophytic bacterial isolates that had the potential to produce cellulase were closely related to 3 genera, namely Bacillus, Amphibacillus, and Micrococcus.
{"title":"Isolation and Identification of Cellulase- Producing Encophytic Bacteria From Yellow Root Plants (Arvangelisia flava (L.) Merr) From Enggano Island","authors":"Stella Reformanda, Sipriyadi, W. Darwis, R. H. Wibowo, R. Supriati, Resli Siboro","doi":"10.2991/absr.k.210621.087","DOIUrl":"https://doi.org/10.2991/absr.k.210621.087","url":null,"abstract":"Endophytic bacteria are bacteria that live in plant tissue without causing disease in their host. In general, endophytic bacteria enter through stomata or wounds in plants by producing cellulase enzymes to degrade cellulose in plant cell walls that contain cellulose, one of which is yellow root (Arcangelisia flava (L.) Merr). The purpose of this study was to isolate endophytic bacteria from yellow roots from Enggano Island, Bengkulu Province, and to identify morphologically, Gram staining and biochemical tests as well as testing the potential of endophytic bacteria in producing cellulase enzymes. Isolation was carried out by the patch method with surface sterilization, using 70% alcohol and 5.25% sodium hypochlorite and then put on NA media that had been treated with Nystatin. The isolates obtained were then tested for their ability to produce cellulase enzymes by scratching them onto CMC media, then measuring the forming of clear zone. The results showed that from 29 isolates, 26 of them were able to degrade cellulose. AKEBG26 and AKEBG25 isolates had a higher ability to hydrolyze cellulose with cellolulitic potential index (IP) were about ±2.90 and ±1.51. Identification based on gram staining and biochemical tests of 8 endophytic bacterial isolates that had the potential to produce cellulase were closely related to 3 genera, namely Bacillus, Amphibacillus, and Micrococcus.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79520794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.029
Nurainas, Eryscha Dwi Syukma, Chairul, Mansyurdin
Mentawai people have a unique agricultural tradition. The traditional agricultural system (mone) is adaptive, using the plants that are suitable for the landscape. Land clearing is carefully done without destroying the entire forest. We conducted ethnobotany studies to explore the composition and the structure of the plants used in the traditional plantation (Pumonean) as well as their values for the Mentawai people in the Siberut island. We surveyed the agricultural system employed by Mentawai people and conducted vegetation assessment in the study sites. The data on the plant species and land usages were gathered from interviews with key informants in the areas. We analyzed the data using Local User’s Value Index (LUVI). The plants were collected to aid with species identification and were stored at the Andalas University Herbarium (ANDA), Padang. Throughout the study, we recorded 64 species in 27 families. Euphorbiaceae was the family with the highest species diversity. Sagu (Metroxylon sago) was the species with the highest LUVI. We categorized the land usage for the traditional plantation into four categories, consisting of wetland, the edge of wetland, mixed plantations (mone), and forests (leleu). The mixed plantation was the category with the highest LUVI. Beside the traditional agricultural system, the Mentawai people also developed a more modern agricultural system. For the traditional system, Durio zibethinus was the species with the highest importance indices, whereas for the more modern system Theobroma cacao was the species with the highest importance indices.
{"title":"Ethnobotanical Aspects of Mentawai Traditional Agricultural System (Pumonean) and Its Implications for the Conservation of Local Germplasm in Siberut, Mentawai, Indonesia","authors":"Nurainas, Eryscha Dwi Syukma, Chairul, Mansyurdin","doi":"10.2991/absr.k.210621.029","DOIUrl":"https://doi.org/10.2991/absr.k.210621.029","url":null,"abstract":"Mentawai people have a unique agricultural tradition. The traditional agricultural system (mone) is adaptive, using the plants that are suitable for the landscape. Land clearing is carefully done without destroying the entire forest. We conducted ethnobotany studies to explore the composition and the structure of the plants used in the traditional plantation (Pumonean) as well as their values for the Mentawai people in the Siberut island. We surveyed the agricultural system employed by Mentawai people and conducted vegetation assessment in the study sites. The data on the plant species and land usages were gathered from interviews with key informants in the areas. We analyzed the data using Local User’s Value Index (LUVI). The plants were collected to aid with species identification and were stored at the Andalas University Herbarium (ANDA), Padang. Throughout the study, we recorded 64 species in 27 families. Euphorbiaceae was the family with the highest species diversity. Sagu (Metroxylon sago) was the species with the highest LUVI. We categorized the land usage for the traditional plantation into four categories, consisting of wetland, the edge of wetland, mixed plantations (mone), and forests (leleu). The mixed plantation was the category with the highest LUVI. Beside the traditional agricultural system, the Mentawai people also developed a more modern agricultural system. For the traditional system, Durio zibethinus was the species with the highest importance indices, whereas for the more modern system Theobroma cacao was the species with the highest importance indices.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78148061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study aims to determine the genetic character of Black-capped white-eye (Zosterops articapilla) using the mtDNA COI gene. Blood sample was collected from bird market in Bengkulu city and its DNA genome isolation and purification following the Protocols of Dneasy® Blood and Tissue Kit Cat no 69504 (50) based on the procedure of Spin-Column Protocol, Qiagen. We used polymerase chain reaction machine for amplification DNA template with specific primer (ZCOIF and ZCOIR). The results showed that there are nucleotide variations on five individual Black-capped white-eye with the COI gene sequence length 750 bp. We found of conservative site (C) is 743 sites, variations (V) seven sites, parsimony (Pi) four sites, singleton (S) three sites, and nucleotide base pairs of Zosterops atricapilla to adenine and thymine (AT) 55.9% and guanine and cytosine (GC) 44.1%. Fifth individual of Black-capped white-eye are separated with average intraspecies genetic distance 0.4%, while its interspecific genetic distance is 3.47% and with the outgroup 12.1%. We think, these COI gene sequences very important to quick identification and could be used as a forensic tool in preventing its trafficking.
{"title":"DNA Barcode of Black Capped White Eye Zosterops atricapilla (Aves: Zosteropidae) From Bengkulu Indonesia Based on Mitochondrial DNA COI Gene","authors":"Jarulis, Ahmat Fakhri Utama, Sipriyadi, Santi Nurul Kamilah","doi":"10.2991/absr.k.210621.061","DOIUrl":"https://doi.org/10.2991/absr.k.210621.061","url":null,"abstract":"The study aims to determine the genetic character of Black-capped white-eye (Zosterops articapilla) using the mtDNA COI gene. Blood sample was collected from bird market in Bengkulu city and its DNA genome isolation and purification following the Protocols of Dneasy® Blood and Tissue Kit Cat no 69504 (50) based on the procedure of Spin-Column Protocol, Qiagen. We used polymerase chain reaction machine for amplification DNA template with specific primer (ZCOIF and ZCOIR). The results showed that there are nucleotide variations on five individual Black-capped white-eye with the COI gene sequence length 750 bp. We found of conservative site (C) is 743 sites, variations (V) seven sites, parsimony (Pi) four sites, singleton (S) three sites, and nucleotide base pairs of Zosterops atricapilla to adenine and thymine (AT) 55.9% and guanine and cytosine (GC) 44.1%. Fifth individual of Black-capped white-eye are separated with average intraspecies genetic distance 0.4%, while its interspecific genetic distance is 3.47% and with the outgroup 12.1%. We think, these COI gene sequences very important to quick identification and could be used as a forensic tool in preventing its trafficking.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78556202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.083
R. H. Wibowo, W. Darwis, Sipriyadi, N. Susianti, Kukuh Andias Purbianto, Gustriana, N. Rosianti, Della Indah Medani, Gustina Dwi Wulandari
Lobster (Panulirus homarus) or spiny lobster is one of the export commodities of the Indonesian fisheries subsector and an important component for shrimp fisheries in Indonesia. This commodity needs to be further developed because of its high trade value and potential. Lobster enlargement cultivation in Indonesia began in early 2000, with the discovery of puerulus attachments in the waters of the bay of Lombok. However, in this lobster cultivation business failure often occurs due to the high mortality caused by infectious diseases which are generally caused by bacterial, parasitic, fungal, and viral infections. The bacteria that infect lobsters can reduce the expected production target. However, bacteria in lobster culture could have beneficial or harmful effects, depending on the prevailing conditions. This study aims to identify bacteria in spiny lobsters (Panulirus homarus). Bacterial isolation came from organs in spiny lobsters, that are hepatopancreas and hemolymph. Bacteria isolates were identified through their colony morphology and biochemical tests. Characterization on the Thiosulfate Citrate Bile Salt Sucrose medium showed that lobsters were infected with Vibrio. Based on Bergey's Manual of Determinative Bacteriology and Nial, the results showed that the Hepatopancreas in first lobsters were infected with Vibrio algynolyticus that mostly in the Hepatopancreas. The second sample was found to be infected with Vibrio damsela that mostly in the Hepatopancreas. However, no bacteria were found in spiny lobster hemolymph.
{"title":"Identification of Pathogenic Bacteria on Spiny Lobster (Panulirus homarus) at Quality Control and Fishery Product Safety Agency (BKIPM) of Bengkulu","authors":"R. H. Wibowo, W. Darwis, Sipriyadi, N. Susianti, Kukuh Andias Purbianto, Gustriana, N. Rosianti, Della Indah Medani, Gustina Dwi Wulandari","doi":"10.2991/absr.k.210621.083","DOIUrl":"https://doi.org/10.2991/absr.k.210621.083","url":null,"abstract":"Lobster (Panulirus homarus) or spiny lobster is one of the export commodities of the Indonesian fisheries subsector and an important component for shrimp fisheries in Indonesia. This commodity needs to be further developed because of its high trade value and potential. Lobster enlargement cultivation in Indonesia began in early 2000, with the discovery of puerulus attachments in the waters of the bay of Lombok. However, in this lobster cultivation business failure often occurs due to the high mortality caused by infectious diseases which are generally caused by bacterial, parasitic, fungal, and viral infections. The bacteria that infect lobsters can reduce the expected production target. However, bacteria in lobster culture could have beneficial or harmful effects, depending on the prevailing conditions. This study aims to identify bacteria in spiny lobsters (Panulirus homarus). Bacterial isolation came from organs in spiny lobsters, that are hepatopancreas and hemolymph. Bacteria isolates were identified through their colony morphology and biochemical tests. Characterization on the Thiosulfate Citrate Bile Salt Sucrose medium showed that lobsters were infected with Vibrio. Based on Bergey's Manual of Determinative Bacteriology and Nial, the results showed that the Hepatopancreas in first lobsters were infected with Vibrio algynolyticus that mostly in the Hepatopancreas. The second sample was found to be infected with Vibrio damsela that mostly in the Hepatopancreas. However, no bacteria were found in spiny lobster hemolymph.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74308231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.049
N. Haneda, A. Ichtisinii, U. Siregar, Y. Istikorini, A. Lestari
{"title":"Chemical Component of Sengon Tree Digested Xystrocera festiva (Coleoptera: Cerambycidae) Larvae","authors":"N. Haneda, A. Ichtisinii, U. Siregar, Y. Istikorini, A. Lestari","doi":"10.2991/absr.k.210621.049","DOIUrl":"https://doi.org/10.2991/absr.k.210621.049","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73806167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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