Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.073
Supanji, A. Perdamaian, Anindita Dianratri, Muhammad Eko Prayogo, M. B. Sasongko, F. S. Wardhana, T. W. Widayanti, A. N. Agni, C. Oka
{"title":"The Circulating Level of IL-1β in Patients with Age-related Macular Degeneration (AMD) in Yogyakarta: Characteristics to Disease Activity","authors":"Supanji, A. Perdamaian, Anindita Dianratri, Muhammad Eko Prayogo, M. B. Sasongko, F. S. Wardhana, T. W. Widayanti, A. N. Agni, C. Oka","doi":"10.2991/absr.k.210621.073","DOIUrl":"https://doi.org/10.2991/absr.k.210621.073","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88708075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.091
W. Darwis, R. Supriati, Sipriyadi, R. H. Wibowo, Yofaldo Umara Al Siddiq
Lichen is one of the organisms with a high level of biodiversity and rich of antibacterial secondary metabolites compounds. One species of lichenes found in Kepahyang Indonesia is Teloschisthes flavicans. This study aims to find out an antibacterial effectiveness of Teloschisthes flavicans extract against the growth of Staphylococcus aureus and Pseudomonas aeruginosa. T. flavicans samples are obtained from the barks of African trees (Maesopsis eminii Engl.), it was then extracted using 96% (1:10) ethanol for 5 days. antibacterial activity was tested using the disc diffusion method with 10 treatments; those are: negative control (DMSO 10%), positive control (chloramphenicol 0.10%), and T. flavicans extract with concentration of 25 ppm; 50 ppm; 75 ppm; 100 ppm; 200 ppm; 400 ppm; 800 ppm; and 1000 ppm. The result obtained the average diameter of the clear zone. Antibacterial activity of T. flavicans against the growth of S. aureus obtained an average diameter of clear zone at 35.30 ± 0.28 mm for the positive control treatment, it was also found the average diameter of the clear zone due to the aplication of T. flavicans extract from the lowest to the highest concentration was that of 8.37 ± 0.10 mm; 14.65 ± 0.91 mm; 11.17 ± 0.10 mm; 11.47 ± 0. mm; 11.90 ± 0.84 mm; 12.50 ± 0.7 mm; 12.22 ± 0.24 mm; and 12.57 ± 0.17 mm. Meanwhile, the result of antibacterial activity of T. flavicans extract against the growth of P. aeruginosa was the average diameter of the clear zone of 37.77 ± 1.02 mm for the positive control, and the clear zone respectively at; 7.02 ± 0.03 mm; 9.00 ± 1.06 mm; 9.97 ± 1.94 mm; 10.95 ± 0.56 mm; 11.50 ±100 ppm 1.27 mm control, and 11.62 ± 1.30 mm; 11.60 ± 0.21 mm; and 12.17 ± 1.02 mm at samples treated by T. flavicans extract from the lowest to the highest concentration. Data was analyzed by ANOVA, followed by the Duncan test using SPSS 16.0. The most effective concentration of T. flavicans extract in inhibiting the growth of S. aureus is 50 ppm. While the most effective concentration of T. flavicans extract in inhibiting the growth of P. aeruginosa was 100 ppm.
{"title":"Antibacterial Potency of Lichen Teloschisthes flavicans From Kepahiang District Against Staphylococcus aureus and Pseudomonas aeruginosa","authors":"W. Darwis, R. Supriati, Sipriyadi, R. H. Wibowo, Yofaldo Umara Al Siddiq","doi":"10.2991/absr.k.210621.091","DOIUrl":"https://doi.org/10.2991/absr.k.210621.091","url":null,"abstract":"Lichen is one of the organisms with a high level of biodiversity and rich of antibacterial secondary metabolites compounds. One species of lichenes found in Kepahyang Indonesia is Teloschisthes flavicans. This study aims to find out an antibacterial effectiveness of Teloschisthes flavicans extract against the growth of Staphylococcus aureus and Pseudomonas aeruginosa. T. flavicans samples are obtained from the barks of African trees (Maesopsis eminii Engl.), it was then extracted using 96% (1:10) ethanol for 5 days. antibacterial activity was tested using the disc diffusion method with 10 treatments; those are: negative control (DMSO 10%), positive control (chloramphenicol 0.10%), and T. flavicans extract with concentration of 25 ppm; 50 ppm; 75 ppm; 100 ppm; 200 ppm; 400 ppm; 800 ppm; and 1000 ppm. The result obtained the average diameter of the clear zone. Antibacterial activity of T. flavicans against the growth of S. aureus obtained an average diameter of clear zone at 35.30 ± 0.28 mm for the positive control treatment, it was also found the average diameter of the clear zone due to the aplication of T. flavicans extract from the lowest to the highest concentration was that of 8.37 ± 0.10 mm; 14.65 ± 0.91 mm; 11.17 ± 0.10 mm; 11.47 ± 0. mm; 11.90 ± 0.84 mm; 12.50 ± 0.7 mm; 12.22 ± 0.24 mm; and 12.57 ± 0.17 mm. Meanwhile, the result of antibacterial activity of T. flavicans extract against the growth of P. aeruginosa was the average diameter of the clear zone of 37.77 ± 1.02 mm for the positive control, and the clear zone respectively at; 7.02 ± 0.03 mm; 9.00 ± 1.06 mm; 9.97 ± 1.94 mm; 10.95 ± 0.56 mm; 11.50 ±100 ppm 1.27 mm control, and 11.62 ± 1.30 mm; 11.60 ± 0.21 mm; and 12.17 ± 1.02 mm at samples treated by T. flavicans extract from the lowest to the highest concentration. Data was analyzed by ANOVA, followed by the Duncan test using SPSS 16.0. The most effective concentration of T. flavicans extract in inhibiting the growth of S. aureus is 50 ppm. While the most effective concentration of T. flavicans extract in inhibiting the growth of P. aeruginosa was 100 ppm.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87755358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.025
Hasrianti, Surianti, A. R. S. Putri, Damis, R. Rajab, A. H. Akbar
The suckermouth catfish is one type of fish species which is an invasive species. The occurrence of suckermouth catfish population explosion in the waters of Lake Sidenreng had an impact on the sustainability of marine biodiversity as well as an impact on the production of fishermen's catch. This study aims to determine the effect of suckermouth catfish population explosion on the effectiveness of fish filling and the sustainability status of Sidenreng Lake water management. This research was conducted for three months, from June to August 2020 in Teteaji Village and Wette'e Village, Sidenreng Rappang Regency. This study aims to determine the effect of fishing effectiveness and the status of the sustainability of the management of Lake Sidenreng. The effect of population explosion on the effectiveness of fishing was analyzed descriptively based on a comparison of the production of moderate catches on the sustainability status of Sidenreng Lake management using the RAPFISHMDS (Rapid Appraisal for Fisheries-Multidimensional Scaling) method. Based on the analysis of the suckermouth catfish population explosion in Sidenreng Lake, it has an impact on capture fisheries activities in Lake Sidenreng, namely on fishing gear, fishing operations and production of catches. The status of the sustainability of Sidenreng Lake management based on the RAPFISH MDS sustainability analysis from all dimensional aspects (ecological, economic, social and technological dimensions) shows that the sustainability status of Lake Sidenreng is 51.12 including the fairly sustainable category.
{"title":"The Effect of Suckermouth Catfish (Pterygoplichthys sp.) Population Explosions on the Effectiveness of Fishing and the Sustainability Status of Sidenreng Lake Waters","authors":"Hasrianti, Surianti, A. R. S. Putri, Damis, R. Rajab, A. H. Akbar","doi":"10.2991/absr.k.210621.025","DOIUrl":"https://doi.org/10.2991/absr.k.210621.025","url":null,"abstract":"The suckermouth catfish is one type of fish species which is an invasive species. The occurrence of suckermouth catfish population explosion in the waters of Lake Sidenreng had an impact on the sustainability of marine biodiversity as well as an impact on the production of fishermen's catch. This study aims to determine the effect of suckermouth catfish population explosion on the effectiveness of fish filling and the sustainability status of Sidenreng Lake water management. This research was conducted for three months, from June to August 2020 in Teteaji Village and Wette'e Village, Sidenreng Rappang Regency. This study aims to determine the effect of fishing effectiveness and the status of the sustainability of the management of Lake Sidenreng. The effect of population explosion on the effectiveness of fishing was analyzed descriptively based on a comparison of the production of moderate catches on the sustainability status of Sidenreng Lake management using the RAPFISHMDS (Rapid Appraisal for Fisheries-Multidimensional Scaling) method. Based on the analysis of the suckermouth catfish population explosion in Sidenreng Lake, it has an impact on capture fisheries activities in Lake Sidenreng, namely on fishing gear, fishing operations and production of catches. The status of the sustainability of Sidenreng Lake management based on the RAPFISH MDS sustainability analysis from all dimensional aspects (ecological, economic, social and technological dimensions) shows that the sustainability status of Lake Sidenreng is 51.12 including the fairly sustainable category.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88995550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.052
S. Pratama, I. Ana, B. Retnoaji
Carbonate Hydroxyapatite (CHA) is one of the bioceramic materials that can be used as dental implants. As dental implant material, the biocompatibility of CHA is an important prerequisite factor. This study aims to determine the biocompatibility of CHA on zebrafish embryos. The zebrafish embryos treatment was carried out on 3 hours post-fertilization (hpf) embryos aged. The embryos were exposed to CHA with concentrations of 15 μg/ml, 125 μg/ml, 500 μg/ml, and 2000 μg/ml, respectively. Biocompatibility assessment was carried out by measuring the hatching rate, survival, morphological changes, and embryo physiological performance, respectively. The hatching rate was determined as the number of embryos that hatched at 48, 72, and 96 hpf. The survival rate was determined by the number of live embryos at 24, 48, 72, and 96 hpf, respectively. Moreover, the morphological change was observed on the shape of the embryo’s heart and yolk, as well as the tail-yolk detachment as an indicator of a normal body wall occurrence, respectively. Moreover, the physiological performance was decided base on the heartbeat rate performance of the embryos. The result showed no effect of CHA on the hatching and survival rate as well as embryo morphology in all treatments. All embryos heart laterality was normally formed, and the tail-yolk detachment occurred at the period of 24 hpf, which indicated normal development of the embryo. The heartbeat rate showed no differences between CHA treatment compared to control, where the embryo heartbeat rate in all treatments was more than 200/minute, indicating normal embryos development. In conclusion, the CHA treatment did not cause any measurable development defect on
{"title":"The Effect of Carbonate Hydroxyapatite (CHA) Dental Implant Material on the Early Development of Zebrafish Embryos (Danio rerio)","authors":"S. Pratama, I. Ana, B. Retnoaji","doi":"10.2991/absr.k.210621.052","DOIUrl":"https://doi.org/10.2991/absr.k.210621.052","url":null,"abstract":"Carbonate Hydroxyapatite (CHA) is one of the bioceramic materials that can be used as dental implants. As dental implant material, the biocompatibility of CHA is an important prerequisite factor. This study aims to determine the biocompatibility of CHA on zebrafish embryos. The zebrafish embryos treatment was carried out on 3 hours post-fertilization (hpf) embryos aged. The embryos were exposed to CHA with concentrations of 15 μg/ml, 125 μg/ml, 500 μg/ml, and 2000 μg/ml, respectively. Biocompatibility assessment was carried out by measuring the hatching rate, survival, morphological changes, and embryo physiological performance, respectively. The hatching rate was determined as the number of embryos that hatched at 48, 72, and 96 hpf. The survival rate was determined by the number of live embryos at 24, 48, 72, and 96 hpf, respectively. Moreover, the morphological change was observed on the shape of the embryo’s heart and yolk, as well as the tail-yolk detachment as an indicator of a normal body wall occurrence, respectively. Moreover, the physiological performance was decided base on the heartbeat rate performance of the embryos. The result showed no effect of CHA on the hatching and survival rate as well as embryo morphology in all treatments. All embryos heart laterality was normally formed, and the tail-yolk detachment occurred at the period of 24 hpf, which indicated normal development of the embryo. The heartbeat rate showed no differences between CHA treatment compared to control, where the embryo heartbeat rate in all treatments was more than 200/minute, indicating normal embryos development. In conclusion, the CHA treatment did not cause any measurable development defect on","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81085909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.056
A. Sudomo, L. Pieter
Syzygium cumini included in the family Myrtaceae is one type of plant that is hard to find. The benefits of S. cumini plants include seeds as a cure for diabetes, fruit as an antioxidant and anticancer, fruit skin as a medicine for dysentery and leaves as a reinforcement of teeth and gums. This research aimed to study the effect of seed population sources on the growth of S. cumini seedling. Seed exploration was carried out in 3 different S. cumini habitats, (1) Gunungkidul District (2) Yogyakarta District and (3) Cikampek District. CRD (Complete Random Design) was used with each population consisting of three replications (50 seeds/replication). The seed source showed significantly different results on the growth of S. cumini seedlings (height, diameter, number of leaves, wet weight of stem and leaves and dry weight of stem and leaves). However, the variation of seed sources did not significantly differ on survival, root wet mean, root dry weight, top-root ratio and seed quality index. Height, diameter, number of leaves, comparison starting from the greatest were Gunungkidul (30.42 cm / 2.93 mm / 21.42), Cikampek (24.64 cm / 2.82 mm / 21.06) and Yogyakarta (21.27 cm / 2.45 mm / 19.27). While The stem and leaves wet weight and stem and leaf biomass comparison from the highest were Gunungkidul (6.84 gram, 2.10 gram), Yogyakarta (4.95 gram / 1.59 gram) and Cikampek (4.24 gram / 1.47 gram). Moreover, seeds from the Gunungkidul population produced better growth than other populations. This study's recommendation is to develop rare plants of S. cumini species that will have the best growth by using seeds from the Gunungkidul
桃金娘科中的桃金娘是一种很难找到的植物。小茴香植物的好处包括种子可以治疗糖尿病，果实可以抗氧化和抗癌，果皮可以治疗痢疾，叶子可以加固牙齿和牙龈。本研究旨在研究种子种群来源对小茴香幼苗生长的影响。在Gunungkidul区(2)Yogyakarta区(3)Cikampek区3个不同的cumini生境进行了种子勘探。采用完全随机设计(CRD)，每个群体由3个重复组成(50个种子/重复)。不同种子源对小茴香幼苗生长(株高、株径、叶数、茎叶湿重和茎叶干重)有显著差异。而不同种子源对成活率、根湿均值、根干重、顶根比和种子品质指数的影响不显著。高、径、叶数比较，从最大开始依次为Gunungkidul (30.42 cm / 2.93 mm / 21.42)、Cikampek (24.64 cm / 2.82 mm / 21.06)和Yogyakarta (21.27 cm / 2.45 mm / 19.27)。茎叶湿重和茎叶生物量比较最高的是Gunungkidul (6.84 g, 2.10 g)、Yogyakarta (4.95 g / 1.59 g)和Cikampek (4.24 g / 1.47 g)。此外，Gunungkidul种群的种子比其他种群的种子生长得更好。本研究建议利用Gunungkidul的种子，开发出生长最好的稀有植物
{"title":"Effect of Seed Source Variation on Syzygium cumini (L.) Skeels. Seedlings Growth in Ciamis District, West Java Province","authors":"A. Sudomo, L. Pieter","doi":"10.2991/absr.k.210621.056","DOIUrl":"https://doi.org/10.2991/absr.k.210621.056","url":null,"abstract":"Syzygium cumini included in the family Myrtaceae is one type of plant that is hard to find. The benefits of S. cumini plants include seeds as a cure for diabetes, fruit as an antioxidant and anticancer, fruit skin as a medicine for dysentery and leaves as a reinforcement of teeth and gums. This research aimed to study the effect of seed population sources on the growth of S. cumini seedling. Seed exploration was carried out in 3 different S. cumini habitats, (1) Gunungkidul District (2) Yogyakarta District and (3) Cikampek District. CRD (Complete Random Design) was used with each population consisting of three replications (50 seeds/replication). The seed source showed significantly different results on the growth of S. cumini seedlings (height, diameter, number of leaves, wet weight of stem and leaves and dry weight of stem and leaves). However, the variation of seed sources did not significantly differ on survival, root wet mean, root dry weight, top-root ratio and seed quality index. Height, diameter, number of leaves, comparison starting from the greatest were Gunungkidul (30.42 cm / 2.93 mm / 21.42), Cikampek (24.64 cm / 2.82 mm / 21.06) and Yogyakarta (21.27 cm / 2.45 mm / 19.27). While The stem and leaves wet weight and stem and leaf biomass comparison from the highest were Gunungkidul (6.84 gram, 2.10 gram), Yogyakarta (4.95 gram / 1.59 gram) and Cikampek (4.24 gram / 1.47 gram). Moreover, seeds from the Gunungkidul population produced better growth than other populations. This study's recommendation is to develop rare plants of S. cumini species that will have the best growth by using seeds from the Gunungkidul","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87529712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.088
Surianti, Fitratul Muaddama, R. S. Putri, Hasrianti, Damis, Wahyudi
Rice bran is the result of the process of milling rice plants into rice, but rice bran has not been utilized properly in the Sidrap area. Feeding with the addition of fermented rice bran using Lactobacillus sp. is expected to increase the use of feed in tilapia aquaculture. This research aims to realize the fermentation of rice bran on the survival parameters and feed conversion ratio (fcr) of tilapia. This research used a completely randomized design with three replications and four treatments, namely (A) 10% dose of rice bran in feed, (B) 15% dose of rice bran in feed, (C) 20% dose of rice bran in feed, and (D) control. feed. The size of the tilapia used is 1.5 g / fish with a density of 20 individuals / 85 L of freshwater. The number of feeding four times a day and given a test feed of 5% of the bodyweight of the fish. The results showed that the bran flour was fermented using Lactobacillus sp. Significantly different in the survival rate and the ratio of tilapia feed conversion and water quality during the study is suitable for use in tilapia fish maintenance. The highest value resulting from the addition of fermented rice bran 15% (18.33) and 20% (19.33) for survival, while the lowest for control (16.67) and feed conversion ratio showed the best results with the provision of fermented rice bran 20 % (3.03).
{"title":"Application of Fermented Rice Bran Using Lactobacillus sp. in Artificial Feed For Survival Rate and FCR of Tilapia (Oreochromis niloticus)","authors":"Surianti, Fitratul Muaddama, R. S. Putri, Hasrianti, Damis, Wahyudi","doi":"10.2991/absr.k.210621.088","DOIUrl":"https://doi.org/10.2991/absr.k.210621.088","url":null,"abstract":"Rice bran is the result of the process of milling rice plants into rice, but rice bran has not been utilized properly in the Sidrap area. Feeding with the addition of fermented rice bran using Lactobacillus sp. is expected to increase the use of feed in tilapia aquaculture. This research aims to realize the fermentation of rice bran on the survival parameters and feed conversion ratio (fcr) of tilapia. This research used a completely randomized design with three replications and four treatments, namely (A) 10% dose of rice bran in feed, (B) 15% dose of rice bran in feed, (C) 20% dose of rice bran in feed, and (D) control. feed. The size of the tilapia used is 1.5 g / fish with a density of 20 individuals / 85 L of freshwater. The number of feeding four times a day and given a test feed of 5% of the bodyweight of the fish. The results showed that the bran flour was fermented using Lactobacillus sp. Significantly different in the survival rate and the ratio of tilapia feed conversion and water quality during the study is suitable for use in tilapia fish maintenance. The highest value resulting from the addition of fermented rice bran 15% (18.33) and 20% (19.33) for survival, while the lowest for control (16.67) and feed conversion ratio showed the best results with the provision of fermented rice bran 20 % (3.03).","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"92 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74208293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.071
T. Novianti, Tazkia Ayu Safitri, F. D. Wahyuni, I. Jamilah, S. Ilyas
Some genes play a role in the tissue regeneration process and they have specific functions. One of them is the platelet-derived growth factors (PDGF) gene that have a role in the wound healing process because it induces cell mitosis and thereby causes cell proliferation. PDGF protein expression is thought to chemotactically recruit many cell types including smooth muscle, fibroblasts, and monocytes to migrate to the wound site. These research aims are to analyze the role of PDGF gene expression in the process of digit tip mice tissue regeneration. This study used 24 mice (Mus musculus) were amputated at the digit tip and the growth was observed on days 0, 1, 3, 5, 10, 15, and 25. We isolated RNA from each tissue after amputation on days 0, 1, 3, 5, 10, 15, and 25 and analyzed the RNA expression of the PDGF gene using the qPCR method. PDGF gene expression reached its peak on day 10 on the granule phase and decreased until day 25. The peak expression of the PDGF gene indicates the role of the gene in the tissue regeneration of digit tip mice. The normality test for PDGF gene expression was normally distributed (p> 0, 05) and the variation test between each treatment was different significantly (p <0.05).
{"title":"The Role of PDGF Gene in Tissue Regeneration of Digit Tip Mice (Mus musculus)","authors":"T. Novianti, Tazkia Ayu Safitri, F. D. Wahyuni, I. Jamilah, S. Ilyas","doi":"10.2991/absr.k.210621.071","DOIUrl":"https://doi.org/10.2991/absr.k.210621.071","url":null,"abstract":"Some genes play a role in the tissue regeneration process and they have specific functions. One of them is the platelet-derived growth factors (PDGF) gene that have a role in the wound healing process because it induces cell mitosis and thereby causes cell proliferation. PDGF protein expression is thought to chemotactically recruit many cell types including smooth muscle, fibroblasts, and monocytes to migrate to the wound site. These research aims are to analyze the role of PDGF gene expression in the process of digit tip mice tissue regeneration. This study used 24 mice (Mus musculus) were amputated at the digit tip and the growth was observed on days 0, 1, 3, 5, 10, 15, and 25. We isolated RNA from each tissue after amputation on days 0, 1, 3, 5, 10, 15, and 25 and analyzed the RNA expression of the PDGF gene using the qPCR method. PDGF gene expression reached its peak on day 10 on the granule phase and decreased until day 25. The peak expression of the PDGF gene indicates the role of the gene in the tissue regeneration of digit tip mice. The normality test for PDGF gene expression was normally distributed (p> 0, 05) and the variation test between each treatment was different significantly (p <0.05).","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79794667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.055
Suhartina, Purwantoro, N. Nugrahaeni, M. J. Mejaya
{"title":"Response of Soybean Lines to Drought Stress During Reproductive Phase","authors":"Suhartina, Purwantoro, N. Nugrahaeni, M. J. Mejaya","doi":"10.2991/absr.k.210621.055","DOIUrl":"https://doi.org/10.2991/absr.k.210621.055","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73680420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.063
L. Efiyanti, W. Trisunaryanti, Syaiful Bahri, Y. Ni'mah, N. Wulandari, S. Sumbogo
The potential for sand in Indonesia is very abundant because Indonesia has a long coastline than other countries. This research was conducted to explore the potential of beach sand as an alternative raw material to replace commercial silica. To obtain the natural silica, the beach sand was prepared and extracted into mesoporous silica. The reflux method was chosen to extract silica using NaOH solution, HCl solution, and CTAB surfactant. The mesoporous structure was potentially affected by CTAB and SiO2 ratio, and therefore, this research was focused more on the effect of CTAB weight variation (3; 4,5 and 6 g). Several instruments were used to analyze the characteristics of silica, such as Surface Area Analyzer (SAA), Scanning Electron Microscopy (SEM), and Fourier Transform Infrared Spectroscopy (FTIR). The mesoporous silica has siloxane (Si-O-Si) and silanol (SiOH) functional group and showed a coral reef-like structure. The porosity properties of these materials ranging from 300-385 m2/g for the specific surface area, 5-8.8 nanometer for pore diameter, and 0.4-0.8 cm3/g for pore volume. Optimal CTAB weight found to be at 6 g, which produces the mesoporous silica with a surface area of 385.928 m2/g, an average pore diameter of 6.015 nanometers, and pore volume of 0.579 cm3/g.
{"title":"Synthesis of Mesoporous Silica From Beach Sand Using Variation of Cetyl Trimethyl Ammonium Bromide (CTAB)","authors":"L. Efiyanti, W. Trisunaryanti, Syaiful Bahri, Y. Ni'mah, N. Wulandari, S. Sumbogo","doi":"10.2991/absr.k.210621.063","DOIUrl":"https://doi.org/10.2991/absr.k.210621.063","url":null,"abstract":"The potential for sand in Indonesia is very abundant because Indonesia has a long coastline than other countries. This research was conducted to explore the potential of beach sand as an alternative raw material to replace commercial silica. To obtain the natural silica, the beach sand was prepared and extracted into mesoporous silica. The reflux method was chosen to extract silica using NaOH solution, HCl solution, and CTAB surfactant. The mesoporous structure was potentially affected by CTAB and SiO2 ratio, and therefore, this research was focused more on the effect of CTAB weight variation (3; 4,5 and 6 g). Several instruments were used to analyze the characteristics of silica, such as Surface Area Analyzer (SAA), Scanning Electron Microscopy (SEM), and Fourier Transform Infrared Spectroscopy (FTIR). The mesoporous silica has siloxane (Si-O-Si) and silanol (SiOH) functional group and showed a coral reef-like structure. The porosity properties of these materials ranging from 300-385 m2/g for the specific surface area, 5-8.8 nanometer for pore diameter, and 0.4-0.8 cm3/g for pore volume. Optimal CTAB weight found to be at 6 g, which produces the mesoporous silica with a surface area of 385.928 m2/g, an average pore diameter of 6.015 nanometers, and pore volume of 0.579 cm3/g.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"166 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84648770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.041
D. Lestari, Fatimatuzzahra
The demand for cultivated fish for public consumption has increased. One of the fish farming methods was a fish pond that is integrated with poultry, especially the types of fish that are easy to cultivate such as tilapia (Oreochromis niloticus) and catfish (Clarias sp.). The aim of this research was to determine the hematological analysis of tilapia and catfish cultivated in the integrated fish farming. The result of the study, physicochemical water parameter showed that the pond has a low pH ± 3,4 and dissolved oxygen ± 2,6 ppm. The tilapia fish had erythrocyte levels of 20,34 x10 cells/mm and leukocyte levels of 189,6x10 cells/mm while the catfish had erythrocyte levels of 29,62x10 cells/mm and leukocyte levels of 113,7x10 cells/mm. Leukocyte and erythrocyte levels in both fish have a different response. The water environment with the integrated fish farming system is shown to have a low pH because of chicken manure and feed residue that entered the fish pond, which is causing the nitrification process and causing the dissolved oxygen to be very low.
{"title":"Hematological Analysis of Oreochromis niloticus and Clarias sp. Cultivated in Integrated Fish Farming","authors":"D. Lestari, Fatimatuzzahra","doi":"10.2991/absr.k.210621.041","DOIUrl":"https://doi.org/10.2991/absr.k.210621.041","url":null,"abstract":"The demand for cultivated fish for public consumption has increased. One of the fish farming methods was a fish pond that is integrated with poultry, especially the types of fish that are easy to cultivate such as tilapia (Oreochromis niloticus) and catfish (Clarias sp.). The aim of this research was to determine the hematological analysis of tilapia and catfish cultivated in the integrated fish farming. The result of the study, physicochemical water parameter showed that the pond has a low pH ± 3,4 and dissolved oxygen ± 2,6 ppm. The tilapia fish had erythrocyte levels of 20,34 x10 cells/mm and leukocyte levels of 189,6x10 cells/mm while the catfish had erythrocyte levels of 29,62x10 cells/mm and leukocyte levels of 113,7x10 cells/mm. Leukocyte and erythrocyte levels in both fish have a different response. The water environment with the integrated fish farming system is shown to have a low pH because of chicken manure and feed residue that entered the fish pond, which is causing the nitrification process and causing the dissolved oxygen to be very low.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73542405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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