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Antimicrobial Activity of Ethanol Extract of Akar Kaik-kaik (Uncaria cordata (Lour.) Merr.) Leaves Against Staphylococcus aureus, Escherichia coli, and Candida albicans 木贼(Uncaria cordata, Lour.)乙醇提取物的抑菌活性稳定)。抗金黄色葡萄球菌,大肠杆菌和白色念珠菌
Vilya Syafriana, Erwi Putri Setyaningsih, Nabilah Rachmawani, D. Kharisma, F. Hamida
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引用次数: 0
Synthesis of Mesoporous Silica From Beach Sand Using Variation of Cetyl Trimethyl Ammonium Bromide (CTAB) 用十六烷基三甲基溴化铵(CTAB)改性沙滩砂合成介孔二氧化硅
L. Efiyanti, W. Trisunaryanti, Syaiful Bahri, Y. Ni'mah, N. Wulandari, S. Sumbogo
The potential for sand in Indonesia is very abundant because Indonesia has a long coastline than other countries. This research was conducted to explore the potential of beach sand as an alternative raw material to replace commercial silica. To obtain the natural silica, the beach sand was prepared and extracted into mesoporous silica. The reflux method was chosen to extract silica using NaOH solution, HCl solution, and CTAB surfactant. The mesoporous structure was potentially affected by CTAB and SiO2 ratio, and therefore, this research was focused more on the effect of CTAB weight variation (3; 4,5 and 6 g). Several instruments were used to analyze the characteristics of silica, such as Surface Area Analyzer (SAA), Scanning Electron Microscopy (SEM), and Fourier Transform Infrared Spectroscopy (FTIR). The mesoporous silica has siloxane (Si-O-Si) and silanol (SiOH) functional group and showed a coral reef-like structure. The porosity properties of these materials ranging from 300-385 m2/g for the specific surface area, 5-8.8 nanometer for pore diameter, and 0.4-0.8 cm3/g for pore volume. Optimal CTAB weight found to be at 6 g, which produces the mesoporous silica with a surface area of 385.928 m2/g, an average pore diameter of 6.015 nanometers, and pore volume of 0.579 cm3/g.
印尼的沙子储量非常丰富,因为印尼的海岸线比其他国家都长。本研究旨在探索沙滩砂作为替代商业二氧化硅原料的潜力。为获得天然二氧化硅,对沙滩砂进行了制备并提取成介孔二氧化硅。采用NaOH溶液、HCl溶液和CTAB表面活性剂回流法提取二氧化硅。CTAB和SiO2的比例可能会影响介孔结构,因此,本研究更多地关注CTAB重量变化对介孔结构的影响(3;采用表面积分析仪(SAA)、扫描电镜(SEM)、傅立叶变换红外光谱(FTIR)等仪器分析了二氧化硅的特性。介孔二氧化硅具有硅氧烷(Si-O-Si)和硅醇(SiOH)官能团,呈珊瑚礁状结构。这些材料的孔隙率在300-385 m2/g的比表面积、5-8.8纳米的孔径和0.4-0.8 cm3/g的孔体积之间变化。CTAB质量为6 g时,制得的介孔二氧化硅的比表面积为385.928 m2/g,平均孔径为6.015 nm,孔体积为0.579 cm3/g。
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引用次数: 0
Seed Collection Time Effect on the Germination Rate and Growth of Rubber Tree Rootstock 采种时间对橡胶树砧木发芽率和生长的影响
Junaidi, Atminingsih, M. Andriyanto
The bud-grafting method is the most economical technique of Hevea brasiliensis propagation. This technique requires rootstock and scion. The high rootstock demand for the replanting program drives the seeds’ suppliers to collect assorted seeds from cultivated fields, lead to a high variation in the germination rate. The time from seed dehiscent to seeding was hypothesized to be crucial inflicting an inconsistency in the germination rate and seedling rootstock growth. This present study used rubber seeds of the GT1 clone which were laid under trees’ shading to mimic the natural environment. Five laying periods were applied i.e., P0 (directly germinated, control), P1 (laid for one week), P2 (laid for two weeks), P3 (laid for three weeks), and P4 (laid for four weeks). Our study implied that the direct sowing after dehiscent was the best way to attain a high germination rate (GR) (51.67%) while delaying seed sowing for one week reduced the GR up to 22.04%. The longer delaying in P2, P3, and P4 did not show significant differences among them which were 13.33%, 1.19%, and 4.35% respectively. However, the seedling showed a similar growth rate including root/shoot ratio regardless of the laying period treatment. The necessity of establishing seed gardens for rootstock was highlighted in which the harvesting of the seeds could be controlled.
嫁接芽法是巴西橡胶树最经济的繁殖技术。这种技术需要砧木和接穗。重新种植计划对砧木的高需求促使种子供应商从耕地中收集各种种子,导致发芽率的高变化。假设种子从开裂到播种的时间是造成发芽率和幼苗砧木生长不一致的关键。本研究利用GT1无性系的橡胶种子,在树荫下放置,模拟自然环境。采用5个产蛋期,即P0(直接发芽,对照)、P1(产蛋1周)、P2(产蛋2周)、P3(产蛋3周)、P4(产蛋4周)。研究结果表明,裂后直接播种是获得高发芽率(51.67%)的最佳方式,延迟播种一周可使发芽率降低22.04%。延迟时间较长的P2、P3、P4三者间差异不显著,分别为13.33%、1.19%、4.35%。然而,无论产蛋期处理,幼苗的生长速率包括根冠比都是相似的。强调了建立根茎种子园的必要性,这样可以控制种子的收获。
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引用次数: 2
Cloning and Expression of Recombinant Protein MPT63 of Mycobacterium tuberculosis Indonesian Isolate as Serodiagnostic Latent Tuberculosis 印尼结核分枝杆菌血清诊断潜伏结核重组蛋白MPT63的克隆与表达
R. Agus, I. I. Fidhatami, M. Hatta
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引用次数: 0
The Effect of Carbonate Hydroxyapatite (CHA) Dental Implant Material on the Early Development of Zebrafish Embryos (Danio rerio) 碳酸羟基磷灰石(CHA)牙种植材料对斑马鱼胚胎早期发育的影响
S. Pratama, I. Ana, B. Retnoaji
Carbonate Hydroxyapatite (CHA) is one of the bioceramic materials that can be used as dental implants. As dental implant material, the biocompatibility of CHA is an important prerequisite factor. This study aims to determine the biocompatibility of CHA on zebrafish embryos. The zebrafish embryos treatment was carried out on 3 hours post-fertilization (hpf) embryos aged. The embryos were exposed to CHA with concentrations of 15 μg/ml, 125 μg/ml, 500 μg/ml, and 2000 μg/ml, respectively. Biocompatibility assessment was carried out by measuring the hatching rate, survival, morphological changes, and embryo physiological performance, respectively. The hatching rate was determined as the number of embryos that hatched at 48, 72, and 96 hpf. The survival rate was determined by the number of live embryos at 24, 48, 72, and 96 hpf, respectively. Moreover, the morphological change was observed on the shape of the embryo’s heart and yolk, as well as the tail-yolk detachment as an indicator of a normal body wall occurrence, respectively. Moreover, the physiological performance was decided base on the heartbeat rate performance of the embryos. The result showed no effect of CHA on the hatching and survival rate as well as embryo morphology in all treatments. All embryos heart laterality was normally formed, and the tail-yolk detachment occurred at the period of 24 hpf, which indicated normal development of the embryo. The heartbeat rate showed no differences between CHA treatment compared to control, where the embryo heartbeat rate in all treatments was more than 200/minute, indicating normal embryos development. In conclusion, the CHA treatment did not cause any measurable development defect on
碳酸羟基磷灰石(CHA)是一种可用于种植牙的生物陶瓷材料。CHA作为种植体材料,其生物相容性是重要的先决条件。本研究旨在确定CHA对斑马鱼胚胎的生物相容性。斑马鱼胚胎处理是在受精后3小时(hpf)胚胎老化时进行的。CHA浓度分别为15、125、500、2000 μg/ml。生物相容性评价分别通过测定孵化率、存活率、形态变化和胚胎生理性能进行。以48,72和96 hpf孵化的胚胎数来确定孵化率。分别在24、48、72和96 hpf时测定活胚数的存活率。此外,胚胎心脏和卵黄的形状以及作为正常体壁发生标志的尾卵黄脱离也发生了形态学变化。此外,生理性能是根据胚胎的心率性能来决定的。结果表明,在所有处理中,CHA对孵化率、成活率和胚胎形态均无影响。胚胎心脏侧边发育正常,24 hpf时出现尾蛋黄脱离,表明胚胎发育正常。与对照组相比,CHA处理的胚胎心跳速率没有差异,所有处理的胚胎心跳速率都大于200次/分钟,表明胚胎发育正常。总之,CHA处理没有造成任何可测量的发育缺陷
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引用次数: 1
Antibacterial Potency of Lichen Teloschisthes flavicans From Kepahiang District Against Staphylococcus aureus and Pseudomonas aeruginosa 柯柏江地区黄底裂苔对金黄色葡萄球菌和铜绿假单胞菌的抑菌作用
W. Darwis, R. Supriati, Sipriyadi, R. H. Wibowo, Yofaldo Umara Al Siddiq
Lichen is one of the organisms with a high level of biodiversity and rich of antibacterial secondary metabolites compounds. One species of lichenes found in Kepahyang Indonesia is Teloschisthes flavicans. This study aims to find out an antibacterial effectiveness of Teloschisthes flavicans extract against the growth of Staphylococcus aureus and Pseudomonas aeruginosa. T. flavicans samples are obtained from the barks of African trees (Maesopsis eminii Engl.), it was then extracted using 96% (1:10) ethanol for 5 days. antibacterial activity was tested using the disc diffusion method with 10 treatments; those are: negative control (DMSO 10%), positive control (chloramphenicol 0.10%), and T. flavicans extract with concentration of 25 ppm; 50 ppm; 75 ppm; 100 ppm; 200 ppm; 400 ppm; 800 ppm; and 1000 ppm. The result obtained the average diameter of the clear zone. Antibacterial activity of T. flavicans against the growth of S. aureus obtained an average diameter of clear zone at 35.30 ± 0.28 mm for the positive control treatment, it was also found the average diameter of the clear zone due to the aplication of T. flavicans extract from the lowest to the highest concentration was that of 8.37 ± 0.10 mm; 14.65 ± 0.91 mm; 11.17 ± 0.10 mm; 11.47 ± 0. mm; 11.90 ± 0.84 mm; 12.50 ± 0.7 mm; 12.22 ± 0.24 mm; and 12.57 ± 0.17 mm. Meanwhile, the result of antibacterial activity of T. flavicans extract against the growth of P. aeruginosa was the average diameter of the clear zone of 37.77 ± 1.02 mm for the positive control, and the clear zone respectively at; 7.02 ± 0.03 mm; 9.00 ± 1.06 mm; 9.97 ± 1.94 mm; 10.95 ± 0.56 mm; 11.50 ±100 ppm 1.27 mm control, and 11.62 ± 1.30 mm; 11.60 ± 0.21 mm; and 12.17 ± 1.02 mm at samples treated by T. flavicans extract from the lowest to the highest concentration. Data was analyzed by ANOVA, followed by the Duncan test using SPSS 16.0. The most effective concentration of T. flavicans extract in inhibiting the growth of S. aureus is 50 ppm. While the most effective concentration of T. flavicans extract in inhibiting the growth of P. aeruginosa was 100 ppm.
地衣是生物多样性高、抗菌次生代谢产物丰富的生物之一。印度尼西亚Kepahyang发现的地衣之一种是Teloschisthes flavicans。本研究旨在研究黄鳍鱼提取物对金黄色葡萄球菌和铜绿假单胞菌生长的抑菌作用。黄曲霉样品取自非洲树木(Maesopsis eminii Engl.)的树皮,然后用96%(1:10)乙醇提取5天。采用圆盘扩散法测定10个处理的抑菌活性;分别为阴性对照(DMSO 10%)、阳性对照(氯霉素0.10%)和浓度为25ppm的黄曲霉提取物;50 ppm;75 ppm;100 ppm;200 ppm;400 ppm;800 ppm;1000ppm。结果得到了清管区的平均直径。黄曲霉对金黄色葡萄球菌生长的抑菌活性得到阳性对照处理的平均透明带直径为35.30±0.28 mm,同时发现黄曲霉提取物从最低浓度到最高浓度的平均透明带直径为8.37±0.10 mm;14.65±0.91 mm;11.17±0.10 mm;11.47±0。mm;11.90±0.84 mm;12.50±0.7 mm;12.22±0.24 mm;12.57±0.17 mm。同时,黄曲霉提取物对铜绿假单胞菌生长的抑制作用为:阳性对照的透明区平均直径为37.77±1.02 mm;7.02±0.03 mm;9.00±1.06 mm;9.97±1.94 mm;10.95±0.56 mm;11.50±100 PPM控制1.27 mm,和11.62±1.30 mm;11.60±0.21 mm;黄曲霉提取物处理的样品,从最低浓度到最高浓度依次为12.17±1.02 mm。数据采用方差分析,采用SPSS 16.0进行Duncan检验。黄曲霉提取物抑制金黄色葡萄球菌生长的最有效浓度为50 ppm。黄曲霉提取物抑制铜绿假单胞菌生长的最有效浓度为100 ppm。
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引用次数: 2
The Effect of Suckermouth Catfish (Pterygoplichthys sp.) Population Explosions on the Effectiveness of Fishing and the Sustainability Status of Sidenreng Lake Waters 吸嘴鲶鱼(Pterygoplichthys sp.)种群爆炸对四登人湖水域捕捞效果及可持续性的影响
Hasrianti, Surianti, A. R. S. Putri, Damis, R. Rajab, A. H. Akbar
The suckermouth catfish is one type of fish species which is an invasive species. The occurrence of suckermouth catfish population explosion in the waters of Lake Sidenreng had an impact on the sustainability of marine biodiversity as well as an impact on the production of fishermen's catch. This study aims to determine the effect of suckermouth catfish population explosion on the effectiveness of fish filling and the sustainability status of Sidenreng Lake water management. This research was conducted for three months, from June to August 2020 in Teteaji Village and Wette'e Village, Sidenreng Rappang Regency. This study aims to determine the effect of fishing effectiveness and the status of the sustainability of the management of Lake Sidenreng. The effect of population explosion on the effectiveness of fishing was analyzed descriptively based on a comparison of the production of moderate catches on the sustainability status of Sidenreng Lake management using the RAPFISHMDS (Rapid Appraisal for Fisheries-Multidimensional Scaling) method. Based on the analysis of the suckermouth catfish population explosion in Sidenreng Lake, it has an impact on capture fisheries activities in Lake Sidenreng, namely on fishing gear, fishing operations and production of catches. The status of the sustainability of Sidenreng Lake management based on the RAPFISH MDS sustainability analysis from all dimensional aspects (ecological, economic, social and technological dimensions) shows that the sustainability status of Lake Sidenreng is 51.12 including the fairly sustainable category.
吸盘鲶鱼是一种外来入侵物种。寺登仁湖水域发生的吸嘴鲶鱼种群爆炸,不仅影响了海洋生物多样性的可持续性,也影响了渔民的捕捞产量。本研究旨在确定吸嘴鲶鱼种群爆炸对鱼充填效果的影响以及四登人湖水体管理的可持续性状况。本研究于2020年6月至8月在西登楞拉邦县的Teteaji村和Wette'e村进行了为期三个月的研究。本研究旨在确定四登人湖渔业效益的影响及管理的可持续性状况。采用RAPFISHMDS(快速渔业评价-多维尺度)方法,在比较四登人湖适度捕捞产量对湖泊管理可持续性状况的基础上,描述性地分析了种群爆炸对捕捞有效性的影响。通过对寺登人湖吸嘴鲶鱼种群爆炸的分析,认为它对寺登人湖捕捞渔业活动产生了影响,即对渔具、捕捞作业和渔获产量产生了影响。基于RAPFISH MDS从生态、经济、社会和技术四个维度的可持续性分析,四登人湖管理的可持续性状况显示,四登人湖的可持续性状况为51.12,其中包括相当可持续的类别。
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引用次数: 0
The Circulating Level of IL-1β in Patients with Age-related Macular Degeneration (AMD) in Yogyakarta: Characteristics to Disease Activity 日惹地区年龄相关性黄斑变性(AMD)患者循环IL-1β水平:疾病活动性特征
Supanji, A. Perdamaian, Anindita Dianratri, Muhammad Eko Prayogo, M. B. Sasongko, F. S. Wardhana, T. W. Widayanti, A. N. Agni, C. Oka
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引用次数: 0
Application of Fermented Rice Bran Using Lactobacillus sp. in Artificial Feed For Survival Rate and FCR of Tilapia (Oreochromis niloticus) 乳酸菌发酵米糠在罗非鱼人工饲料中的应用
Surianti, Fitratul Muaddama, R. S. Putri, Hasrianti, Damis, Wahyudi
Rice bran is the result of the process of milling rice plants into rice, but rice bran has not been utilized properly in the Sidrap area. Feeding with the addition of fermented rice bran using Lactobacillus sp. is expected to increase the use of feed in tilapia aquaculture. This research aims to realize the fermentation of rice bran on the survival parameters and feed conversion ratio (fcr) of tilapia. This research used a completely randomized design with three replications and four treatments, namely (A) 10% dose of rice bran in feed, (B) 15% dose of rice bran in feed, (C) 20% dose of rice bran in feed, and (D) control. feed. The size of the tilapia used is 1.5 g / fish with a density of 20 individuals / 85 L of freshwater. The number of feeding four times a day and given a test feed of 5% of the bodyweight of the fish. The results showed that the bran flour was fermented using Lactobacillus sp. Significantly different in the survival rate and the ratio of tilapia feed conversion and water quality during the study is suitable for use in tilapia fish maintenance. The highest value resulting from the addition of fermented rice bran 15% (18.33) and 20% (19.33) for survival, while the lowest for control (16.67) and feed conversion ratio showed the best results with the provision of fermented rice bran 20 % (3.03).
米糠是稻谷碾磨成大米的产物,但米糠在Sidrap地区还没有得到合理利用。在罗非鱼养殖中添加乳杆菌发酵米糠有望提高饲料的利用率。本研究旨在研究米糠发酵对罗非鱼生存参数和饲料系数的影响。本研究采用完全随机设计,3个重复,4个处理,即(a)饲料中添加10%米糠,(B)饲料中添加15%米糠,(C)饲料中添加20%米糠,(D)对照。饲料。所使用罗非鱼的大小为1.5克/条,密度为20只/ 85升淡水。每天投喂4次,并给予鱼体重5%的试验饲料。结果表明,采用乳酸杆菌发酵的麸皮粉在研究过程中对罗非鱼的成活率、饲料转化率和水质有显著影响,适合用于罗非鱼的养护。发酵米糠添加量为15%(18.33)和20%(19.33)时,成活率最高,对照组最低(16.67),饲料系数以添加量为20%(3.03)时效果最佳。
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引用次数: 6
Activity of Ethanol-derived Fraction of Clove Leaves and Eugenol Compound as Antiaging Agent in the Yeast Model Organism Schizosaccharomyces pombe 丁香叶乙醇提取物和丁香酚化合物在酵母模式生物裂糖酵母中的抗衰老活性
S. Anwar, R. Astuti, D. Solihin
{"title":"Activity of Ethanol-derived Fraction of Clove Leaves and Eugenol Compound as Antiaging Agent in the Yeast Model Organism Schizosaccharomyces pombe","authors":"S. Anwar, R. Astuti, D. Solihin","doi":"10.2991/absr.k.210621.086","DOIUrl":"https://doi.org/10.2991/absr.k.210621.086","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72954011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)
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