首页 > 最新文献

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)最新文献

英文 中文
Effect of Skim Milk on Sperm Motility of Brek Fish, Systomus orphoides (Valenciennes, 1842) for Short Term Preservation 脱脂乳对短期保存鲈鱼(systemus orphoides, valcienes, 1842)精子活力的影响
Rifqi Ramadhana, Abinawanto, R. Gustiano
The purpose of this research was to evaluate the effect of skim milk on sperm motility of Brek fish, Systomus orphoides (Valenciennes, 1842) after 48 hours in 4 ̊C. The method in this research is sperm was collected by hand stripping and was diluted by diluent, which consisted of the fish ringer, skim milk and methanol 10%. The ratio of sperm to diluent is 1:9. The concentrations of skim milk used in this research were 0%, 5%, 10%, 15%, and 20%, respectively. The sperm was stored at 4 ̊C for 48 hours after diluted sperm is thawed in room temperate for 1 minute and sperm motility was observed in improved Neubauer with a light microscope.The motility percentage was analyzed using ANOVA and Tukey test. According to the Annova test (P>0.05), 10% of skim milk showed the highest sperm motility (92.18 + 3.05) % after five replication per dose. Ten percent of skim milk was the optimum concentration to preserve sperm motility at 4 ̊C for 48 hours compared to 0%, 5%, 15%, and 20%. The result of each concentration is 90,85%, 84,68%, 79,54%, and 74,14% respectively.
本研究的目的是评价脱脂乳对Brek fish, systemus orphoides (valencienne, 1842)在4℃条件下48小时精子活力的影响。本研究的方法是用手剥取精子,用稀释剂稀释,稀释剂由鱼环、脱脂牛奶和10%的甲醇组成。精子和稀释剂的比例是1:9。本研究中使用的脱脂牛奶浓度分别为0%、5%、10%、15%和20%。将稀释后的精子室温解冻1分钟后,于4℃保存48 h,光镜下观察改良Neubauer精子活力。运动率分析采用方差分析和Tukey检验。经anova检验(P>0.05), 10%脱脂乳每剂量重复5次后精子活力最高(92.18 + 3.05)%。与0%、5%、15%和20%的脱脂牛奶相比,10%的脱脂牛奶是在4℃条件下保持精子活力48小时的最佳浓度。各浓度测定结果分别为90、85%、84、68%、79、54%和74、14%。
{"title":"Effect of Skim Milk on Sperm Motility of Brek Fish, Systomus orphoides (Valenciennes, 1842) for Short Term Preservation","authors":"Rifqi Ramadhana, Abinawanto, R. Gustiano","doi":"10.2991/absr.k.210621.050","DOIUrl":"https://doi.org/10.2991/absr.k.210621.050","url":null,"abstract":"The purpose of this research was to evaluate the effect of skim milk on sperm motility of Brek fish, Systomus orphoides (Valenciennes, 1842) after 48 hours in 4 ̊C. The method in this research is sperm was collected by hand stripping and was diluted by diluent, which consisted of the fish ringer, skim milk and methanol 10%. The ratio of sperm to diluent is 1:9. The concentrations of skim milk used in this research were 0%, 5%, 10%, 15%, and 20%, respectively. The sperm was stored at 4 ̊C for 48 hours after diluted sperm is thawed in room temperate for 1 minute and sperm motility was observed in improved Neubauer with a light microscope.The motility percentage was analyzed using ANOVA and Tukey test. According to the Annova test (P>0.05), 10% of skim milk showed the highest sperm motility (92.18 + 3.05) % after five replication per dose. Ten percent of skim milk was the optimum concentration to preserve sperm motility at 4 ̊C for 48 hours compared to 0%, 5%, 15%, and 20%. The result of each concentration is 90,85%, 84,68%, 79,54%, and 74,14% respectively.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82901308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Difference in F2 Isoprotane Levels in Male Model Mice (Mus musculus L.) in Maximum Physical Exercise Without Supplementation 最大运动条件下雄性模型小鼠(小家鼠)F2异丙烷水平的差异
E. Yuniarti, M. Des, P. M. Indika
Vigorous physical exercise that exceeds the limit of fatigue can increase the formation of free radicals that lead to oxidative stress. One of the biomarkers used to determine the presence of oxidative stress is the level of F2isoprostane. This study aims to determine the differences in levels of F2-isoprostan in male model mice (Mus musculus L.) in maximum physical exercise without supplementation. This research is an experiment. The population in this study were 30-35gram male mice aged 8-10 weeks. Based on the provisions in the study, the sample was 30 male mice that met the criteria. Examination of samples using ELISA reader and data processed in tables and graphs. The results of statistical tests obtained p value <0.05 (p <0.01), it can be concluded that there is a difference in F2-isoprostane levels between the groups that were not given weight training and those who were given maximum weight training. Where the levels of F2-isoprostane given maximum weight, training were 2 times greater than those not given weight training.
超过疲劳极限的剧烈体育锻炼可以增加自由基的形成,从而导致氧化应激。用于确定氧化应激存在的生物标志物之一是f2异前列腺素的水平。本研究旨在确定雄性模型小鼠(Mus musculus L.)在无补充的最大运动条件下f2 -异前列腺素水平的差异。这项研究是一项实验。本研究以8-10周龄30-35克雄性小鼠为研究对象。根据研究中的规定,样本是30只符合标准的雄性小鼠。使用ELISA阅读器检测样品,数据以表格和图表处理。统计学检验结果p值<0.05 (p <0.01),提示未进行重量训练组与最大重量训练组间f2 -异前列腺素水平存在差异。给予最大重量训练的f2 -异前列腺素水平是未给予重量训练的2倍。
{"title":"Difference in F2 Isoprotane Levels in Male Model Mice (Mus musculus L.) in Maximum Physical Exercise Without Supplementation","authors":"E. Yuniarti, M. Des, P. M. Indika","doi":"10.2991/absr.k.210621.042","DOIUrl":"https://doi.org/10.2991/absr.k.210621.042","url":null,"abstract":"Vigorous physical exercise that exceeds the limit of fatigue can increase the formation of free radicals that lead to oxidative stress. One of the biomarkers used to determine the presence of oxidative stress is the level of F2isoprostane. This study aims to determine the differences in levels of F2-isoprostan in male model mice (Mus musculus L.) in maximum physical exercise without supplementation. This research is an experiment. The population in this study were 30-35gram male mice aged 8-10 weeks. Based on the provisions in the study, the sample was 30 male mice that met the criteria. Examination of samples using ELISA reader and data processed in tables and graphs. The results of statistical tests obtained p value <0.05 (p <0.01), it can be concluded that there is a difference in F2-isoprostane levels between the groups that were not given weight training and those who were given maximum weight training. Where the levels of F2-isoprostane given maximum weight, training were 2 times greater than those not given weight training.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78045325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploration of Phyllosphere Bacteria From Andaliman (Zanthoxylum acanthopodium DC.) 安达曼岛层球细菌的探索(Zanthoxylum acanthopodium DC.)
Debie Rizqoh, Sipriyadi, Shella Sharon, I. Rachmawati, Wulan Okta Kumala, C. N. Putri
Phyllosphere bacteria were bacteria that inhabit around the leaf surface. Several studies showed the potential of phyllosphere bacteria in producing bioactive compounds, one of which is antimicrobial compounds. Andaliman (Zanthoxylum acanthopodium DC.) was a plant from the Rutaceae family that was commonly found in North Sumatra, and its fruit was widely used as a spice in traditional cooking by the Batak tribe. Several studies showed that the andaliman terpenoid has antioxidant and antimicrobial activity and an immunostimulating effect. This study aimed to explore the potential of andaliman phyllosphere bacteria. The research design method was a qualitative experimental model. The research stages used in compiling this paper consisted of phyllosphere bacteria isolation, phyllosphere bacteria morphological characterization, and Gram stain. This study succeeded in isolating 206 colonies of andaliman phyllosphere bacteria with various colony forms and cell morphology. From 64 samples of phyllosphere bacterial isolate observed, we found 25 different colony forms, and all samples were Gram-positive bacteria. Microscopic observations of gram staining showed that 44 isolates (68.75%) were cocci, 17 isolates (26.56%) were in the form of bacillus, and three isolates (4.68%) were in the form of coccobacillus.
叶球细菌是指生活在叶子表面的细菌。一些研究表明,层球菌具有生产生物活性化合物的潜力,其中之一是抗菌化合物。安达利曼(Zanthoxylum acanthopodium DC.)是一种常见于北苏门答腊的rutacae科植物,其果实被巴塔克部落广泛用作传统烹饪中的香料。多项研究表明,安达里曼萜类化合物具有抗氧化、抗菌和免疫刺激作用。本研究旨在探索安达曼叶层球细菌的潜力。研究设计方法为定性实验模型。本文的研究阶段包括:层球细菌分离、层球细菌形态鉴定和革兰氏染色。本研究成功分离出了具有不同菌落形式和细胞形态的206个安达里曼根球菌菌落。从64份根球细菌分离样本中,我们发现25种不同的菌落形式,所有样本均为革兰氏阳性菌。镜下革兰氏染色结果显示,球菌44株(68.75%),芽孢杆菌17株(26.56%),球芽孢杆菌3株(4.68%)。
{"title":"Exploration of Phyllosphere Bacteria From Andaliman (Zanthoxylum acanthopodium DC.)","authors":"Debie Rizqoh, Sipriyadi, Shella Sharon, I. Rachmawati, Wulan Okta Kumala, C. N. Putri","doi":"10.2991/absr.k.210621.075","DOIUrl":"https://doi.org/10.2991/absr.k.210621.075","url":null,"abstract":"Phyllosphere bacteria were bacteria that inhabit around the leaf surface. Several studies showed the potential of phyllosphere bacteria in producing bioactive compounds, one of which is antimicrobial compounds. Andaliman (Zanthoxylum acanthopodium DC.) was a plant from the Rutaceae family that was commonly found in North Sumatra, and its fruit was widely used as a spice in traditional cooking by the Batak tribe. Several studies showed that the andaliman terpenoid has antioxidant and antimicrobial activity and an immunostimulating effect. This study aimed to explore the potential of andaliman phyllosphere bacteria. The research design method was a qualitative experimental model. The research stages used in compiling this paper consisted of phyllosphere bacteria isolation, phyllosphere bacteria morphological characterization, and Gram stain. This study succeeded in isolating 206 colonies of andaliman phyllosphere bacteria with various colony forms and cell morphology. From 64 samples of phyllosphere bacterial isolate observed, we found 25 different colony forms, and all samples were Gram-positive bacteria. Microscopic observations of gram staining showed that 44 isolates (68.75%) were cocci, 17 isolates (26.56%) were in the form of bacillus, and three isolates (4.68%) were in the form of coccobacillus.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88826121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Influence of Green, Corn, and Rice Seed Media Composition on Mycelium Growth of F2 Main Seeds (Pleurotus ostreatus (Jacq. Ex Fr) Kummer) 绿色、玉米和水稻种子培养基组成对F2主种子(平菇)菌丝生长的影响。前主任(Kummer)
W. Darwis, R. H. Wibowo, Sipriyadi, Raden Roro Upiek Ngesti Wibawaning Astuti, Lam Way Sitorus
White oyster mushroom (Pleurotus ostreatus) is a type of fungus wood that has a high nutritional content. White oyster mushroom cultivation begins with the provision of good quality seeds which include 4 stages, namely F1 seeds, F2, F3 and baglog. This study aims to influence the composition of the green bean, maize and rice grain media composition on the growth of mycelium F2 parent white oyster mushroom. This research was conducted at the Microbiology Laboratory, Department of Biology. The research design used a randomized block design (RBD), with the parameters of observing the propagation length, color and thickness of the mycelium. The results showed that the composition of different media had a significant effect on the propagation of mycelium in P2, P9, P10, P18, P19, P20 with the highest length of 14 cm, compact white color and evenly thick on combination media. Environmental conditions suitable for the growth of mycelium seeds F2 white oyster mushroom include temperature 27-28 ̊C, humidity 60-62%, and light intensity 24-54 Lux.
白平菇(Pleurotus ostreatus)是一种营养丰富的木耳木材。白平菇栽培从提供优质种子开始,分为F1种子、F2种子、F3种子和baglog种子4个阶段。本试验旨在研究青豆、玉米和水稻三种培养基组成对F2亲本白平菇菌丝生长的影响。这项研究是在生物系微生物实验室进行的。研究设计采用随机区组设计(RBD),参数为观察菌丝繁殖长度、菌丝颜色和菌丝厚度。结果表明:不同培养基组成对P2、P9、P10、P18、P19、P20菌丝的繁殖影响显著,在组合培养基上菌丝最长可达14 cm,菌丝颜色致密,菌丝粗细均匀;适宜F2白平菇菌丝种子生长的环境条件为温度27 ~ 28℃,湿度60 ~ 62%,光照强度24 ~ 54 Lux。
{"title":"The Influence of Green, Corn, and Rice Seed Media Composition on Mycelium Growth of F2 Main Seeds (Pleurotus ostreatus (Jacq. Ex Fr) Kummer)","authors":"W. Darwis, R. H. Wibowo, Sipriyadi, Raden Roro Upiek Ngesti Wibawaning Astuti, Lam Way Sitorus","doi":"10.2991/absr.k.210621.092","DOIUrl":"https://doi.org/10.2991/absr.k.210621.092","url":null,"abstract":"White oyster mushroom (Pleurotus ostreatus) is a type of fungus wood that has a high nutritional content. White oyster mushroom cultivation begins with the provision of good quality seeds which include 4 stages, namely F1 seeds, F2, F3 and baglog. This study aims to influence the composition of the green bean, maize and rice grain media composition on the growth of mycelium F2 parent white oyster mushroom. This research was conducted at the Microbiology Laboratory, Department of Biology. The research design used a randomized block design (RBD), with the parameters of observing the propagation length, color and thickness of the mycelium. The results showed that the composition of different media had a significant effect on the propagation of mycelium in P2, P9, P10, P18, P19, P20 with the highest length of 14 cm, compact white color and evenly thick on combination media. Environmental conditions suitable for the growth of mycelium seeds F2 white oyster mushroom include temperature 27-28 ̊C, humidity 60-62%, and light intensity 24-54 Lux.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87739766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sperm Motility of Brek Fish (Systomus Orphoides) Using Soy Extract as a Supplementation Extender 大豆提取物对裂口鱼精子活力的影响
Marindha Febriani, Abinawanto, R. R. Dewi
{"title":"Sperm Motility of Brek Fish (Systomus Orphoides) Using Soy Extract as a Supplementation Extender","authors":"Marindha Febriani, Abinawanto, R. R. Dewi","doi":"10.2991/absr.k.210621.047","DOIUrl":"https://doi.org/10.2991/absr.k.210621.047","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87806499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Species and Abundance of Subterranean Termites (Insecta: Isoptera) in the Area of University of Bengkulu 白蚁(昆虫目:等翅目)在蚌库鲁大学地区的种类和数量
Helmiyetti, Darmi, Rezan Okto Wesa
{"title":"The Species and Abundance of Subterranean Termites (Insecta: Isoptera) in the Area of University of Bengkulu","authors":"Helmiyetti, Darmi, Rezan Okto Wesa","doi":"10.2991/absr.k.210621.026","DOIUrl":"https://doi.org/10.2991/absr.k.210621.026","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89397484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Low Reproduction Success of Calliandra calothyrsus and It’s Implication for Breeding calothyrsus的低繁殖成功率及其育种意义
L. Baskorowati, R. Hendrati, N. Hidayati, Mashudi, M. Susanto, D. Setiadi
In anticipating the rapid decrease of energy resource, especially fossil fuel, immediate actions are required to fulfil the needs of highly increased population in the world. Innovation to produce renewable energy from easily utilized raw materials should meet society’s needs including renewable raw materials from wood biomass for energy. Genetically improved Calliandra calothyrsus has been developed for this purpose. Due to low success rate of its reproduction, study on its reproductive biology was undertaken. The study was carried out during the flowering seasons of C. calothyrsus, by examining flower morphology, pollen, and pistil viabilities as wee as insect visitors to this species’ flowers. The study showed that the flowers of C. calothyrsus are borne on inflorescences (spikes) and carrying an average of 354 individual flowers, which develop acropetally. Pistils (female organ) cease to develop by the 2 to 3 day after their appearance, and the flowering is becoming ceased then. Pistils showed maturity during the period of 09.00 WIB 10.00 WIB and pollen is still viable from 1 to 3 days after the pollen opening. Three most frequent visitors (families: Apidae, Vespidae, Formicidae) and one order lepidoptera were found visiting C. calothyrsus flowers However, the reproductive success of C. calotyrsus is found to be very low, which is only 0.024. These preliminary results confirmed that the low reproductive success of C. calothyrsus is due to the structure and development of flowers allowing the incidences of both outcrossing and self-pollination. The limitation time of pistil maturity might also contribute to the low reproductive success of this species.
由于预计能源,特别是矿物燃料将迅速减少,必须立即采取行动,以满足世界上急剧增加的人口的需要。从易于利用的原材料生产可再生能源的创新应满足社会的需求,包括从木材生物质中获取可再生能源。遗传改良的Calliandra calothyrsus已为此目的而开发。由于其繁殖成功率低,对其生殖生物学进行了研究。本研究是在C. calothyrsus的开花季节进行的,通过观察花的形态、花粉和雌蕊的活力,以及昆虫对该物种花的访问。研究结果表明,花在花序(穗状花序)上生长,平均携带354朵单花,花体顶端发育。雌蕊(雌性器官)在出现后2 - 3天停止发育,此时开始停止开花。雌蕊在09:00 WIB ~ 10.00 WIB期间成熟,花粉开放后1 ~ 3 d仍有活力。访花次数最多的有3科(蚜科、蜂科、蚁科)和1目鳞翅目,但其繁殖成功率很低,仅为0.024。这些初步结果证实,卡罗thyrsus的繁殖成功率低是由于花的结构和发育允许异交和自花授粉的发生。雌蕊成熟时间的限制也可能是导致本种繁殖成功率低的原因之一。
{"title":"Low Reproduction Success of Calliandra calothyrsus and It’s Implication for Breeding","authors":"L. Baskorowati, R. Hendrati, N. Hidayati, Mashudi, M. Susanto, D. Setiadi","doi":"10.2991/absr.k.210621.008","DOIUrl":"https://doi.org/10.2991/absr.k.210621.008","url":null,"abstract":"In anticipating the rapid decrease of energy resource, especially fossil fuel, immediate actions are required to fulfil the needs of highly increased population in the world. Innovation to produce renewable energy from easily utilized raw materials should meet society’s needs including renewable raw materials from wood biomass for energy. Genetically improved Calliandra calothyrsus has been developed for this purpose. Due to low success rate of its reproduction, study on its reproductive biology was undertaken. The study was carried out during the flowering seasons of C. calothyrsus, by examining flower morphology, pollen, and pistil viabilities as wee as insect visitors to this species’ flowers. The study showed that the flowers of C. calothyrsus are borne on inflorescences (spikes) and carrying an average of 354 individual flowers, which develop acropetally. Pistils (female organ) cease to develop by the 2 to 3 day after their appearance, and the flowering is becoming ceased then. Pistils showed maturity during the period of 09.00 WIB 10.00 WIB and pollen is still viable from 1 to 3 days after the pollen opening. Three most frequent visitors (families: Apidae, Vespidae, Formicidae) and one order lepidoptera were found visiting C. calothyrsus flowers However, the reproductive success of C. calotyrsus is found to be very low, which is only 0.024. These preliminary results confirmed that the low reproductive success of C. calothyrsus is due to the structure and development of flowers allowing the incidences of both outcrossing and self-pollination. The limitation time of pistil maturity might also contribute to the low reproductive success of this species.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88726717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Response of Soybean Lines to Drought Stress During Reproductive Phase 大豆系生殖期对干旱胁迫的响应
Suhartina, Purwantoro, N. Nugrahaeni, M. J. Mejaya
{"title":"Response of Soybean Lines to Drought Stress During Reproductive Phase","authors":"Suhartina, Purwantoro, N. Nugrahaeni, M. J. Mejaya","doi":"10.2991/absr.k.210621.055","DOIUrl":"https://doi.org/10.2991/absr.k.210621.055","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73680420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Role of PDGF Gene in Tissue Regeneration of Digit Tip Mice (Mus musculus) PDGF基因在指尖小鼠组织再生中的作用
T. Novianti, Tazkia Ayu Safitri, F. D. Wahyuni, I. Jamilah, S. Ilyas
Some genes play a role in the tissue regeneration process and they have specific functions. One of them is the platelet-derived growth factors (PDGF) gene that have a role in the wound healing process because it induces cell mitosis and thereby causes cell proliferation. PDGF protein expression is thought to chemotactically recruit many cell types including smooth muscle, fibroblasts, and monocytes to migrate to the wound site. These research aims are to analyze the role of PDGF gene expression in the process of digit tip mice tissue regeneration. This study used 24 mice (Mus musculus) were amputated at the digit tip and the growth was observed on days 0, 1, 3, 5, 10, 15, and 25. We isolated RNA from each tissue after amputation on days 0, 1, 3, 5, 10, 15, and 25 and analyzed the RNA expression of the PDGF gene using the qPCR method. PDGF gene expression reached its peak on day 10 on the granule phase and decreased until day 25. The peak expression of the PDGF gene indicates the role of the gene in the tissue regeneration of digit tip mice. The normality test for PDGF gene expression was normally distributed (p> 0, 05) and the variation test between each treatment was different significantly (p <0.05).
一些基因在组织再生过程中起作用,具有特定的功能。其中之一是血小板衍生生长因子(PDGF)基因,它在伤口愈合过程中起作用,因为它诱导细胞有丝分裂,从而导致细胞增殖。PDGF蛋白的表达被认为是通过化学方式招募许多细胞类型,包括平滑肌细胞、成纤维细胞和单核细胞迁移到伤口部位。本研究旨在分析PDGF基因表达在指尖小鼠组织再生过程中的作用。本研究采用24只小鼠(小家鼠),分别于第0、1、3、5、10、15、25天进行指端切除,观察其生长情况。我们在截肢后的第0、1、3、5、10、15和25天从每个组织中分离RNA,并使用qPCR方法分析PDGF基因的RNA表达。PDGF基因表达在颗粒期第10天达到峰值,并逐渐降低至第25天。PDGF基因的峰值表达表明该基因在指尖小鼠组织再生中的作用。PDGF基因表达正态性检验呈正态分布(p> 0.05),各组间变异检验差异有统计学意义(p <0.05)。
{"title":"The Role of PDGF Gene in Tissue Regeneration of Digit Tip Mice (Mus musculus)","authors":"T. Novianti, Tazkia Ayu Safitri, F. D. Wahyuni, I. Jamilah, S. Ilyas","doi":"10.2991/absr.k.210621.071","DOIUrl":"https://doi.org/10.2991/absr.k.210621.071","url":null,"abstract":"Some genes play a role in the tissue regeneration process and they have specific functions. One of them is the platelet-derived growth factors (PDGF) gene that have a role in the wound healing process because it induces cell mitosis and thereby causes cell proliferation. PDGF protein expression is thought to chemotactically recruit many cell types including smooth muscle, fibroblasts, and monocytes to migrate to the wound site. These research aims are to analyze the role of PDGF gene expression in the process of digit tip mice tissue regeneration. This study used 24 mice (Mus musculus) were amputated at the digit tip and the growth was observed on days 0, 1, 3, 5, 10, 15, and 25. We isolated RNA from each tissue after amputation on days 0, 1, 3, 5, 10, 15, and 25 and analyzed the RNA expression of the PDGF gene using the qPCR method. PDGF gene expression reached its peak on day 10 on the granule phase and decreased until day 25. The peak expression of the PDGF gene indicates the role of the gene in the tissue regeneration of digit tip mice. The normality test for PDGF gene expression was normally distributed (p> 0, 05) and the variation test between each treatment was different significantly (p <0.05).","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79794667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Seed Source Variation on Syzygium cumini (L.) Skeels. Seedlings Growth in Ciamis District, West Java Province 种子源变异对小茴香的影响斯基尔。西爪哇省Ciamis区的幼苗生长情况
A. Sudomo, L. Pieter
Syzygium cumini included in the family Myrtaceae is one type of plant that is hard to find. The benefits of S. cumini plants include seeds as a cure for diabetes, fruit as an antioxidant and anticancer, fruit skin as a medicine for dysentery and leaves as a reinforcement of teeth and gums. This research aimed to study the effect of seed population sources on the growth of S. cumini seedling. Seed exploration was carried out in 3 different S. cumini habitats, (1) Gunungkidul District (2) Yogyakarta District and (3) Cikampek District. CRD (Complete Random Design) was used with each population consisting of three replications (50 seeds/replication). The seed source showed significantly different results on the growth of S. cumini seedlings (height, diameter, number of leaves, wet weight of stem and leaves and dry weight of stem and leaves). However, the variation of seed sources did not significantly differ on survival, root wet mean, root dry weight, top-root ratio and seed quality index. Height, diameter, number of leaves, comparison starting from the greatest were Gunungkidul (30.42 cm / 2.93 mm / 21.42), Cikampek (24.64 cm / 2.82 mm / 21.06) and Yogyakarta (21.27 cm / 2.45 mm / 19.27). While The stem and leaves wet weight and stem and leaf biomass comparison from the highest were Gunungkidul (6.84 gram, 2.10 gram), Yogyakarta (4.95 gram / 1.59 gram) and Cikampek (4.24 gram / 1.47 gram). Moreover, seeds from the Gunungkidul population produced better growth than other populations. This study's recommendation is to develop rare plants of S. cumini species that will have the best growth by using seeds from the Gunungkidul
桃金娘科中的桃金娘是一种很难找到的植物。小茴香植物的好处包括种子可以治疗糖尿病,果实可以抗氧化和抗癌,果皮可以治疗痢疾,叶子可以加固牙齿和牙龈。本研究旨在研究种子种群来源对小茴香幼苗生长的影响。在Gunungkidul区(2)Yogyakarta区(3)Cikampek区3个不同的cumini生境进行了种子勘探。采用完全随机设计(CRD),每个群体由3个重复组成(50个种子/重复)。不同种子源对小茴香幼苗生长(株高、株径、叶数、茎叶湿重和茎叶干重)有显著差异。而不同种子源对成活率、根湿均值、根干重、顶根比和种子品质指数的影响不显著。高、径、叶数比较,从最大开始依次为Gunungkidul (30.42 cm / 2.93 mm / 21.42)、Cikampek (24.64 cm / 2.82 mm / 21.06)和Yogyakarta (21.27 cm / 2.45 mm / 19.27)。茎叶湿重和茎叶生物量比较最高的是Gunungkidul (6.84 g, 2.10 g)、Yogyakarta (4.95 g / 1.59 g)和Cikampek (4.24 g / 1.47 g)。此外,Gunungkidul种群的种子比其他种群的种子生长得更好。本研究建议利用Gunungkidul的种子,开发出生长最好的稀有植物
{"title":"Effect of Seed Source Variation on Syzygium cumini (L.) Skeels. Seedlings Growth in Ciamis District, West Java Province","authors":"A. Sudomo, L. Pieter","doi":"10.2991/absr.k.210621.056","DOIUrl":"https://doi.org/10.2991/absr.k.210621.056","url":null,"abstract":"Syzygium cumini included in the family Myrtaceae is one type of plant that is hard to find. The benefits of S. cumini plants include seeds as a cure for diabetes, fruit as an antioxidant and anticancer, fruit skin as a medicine for dysentery and leaves as a reinforcement of teeth and gums. This research aimed to study the effect of seed population sources on the growth of S. cumini seedling. Seed exploration was carried out in 3 different S. cumini habitats, (1) Gunungkidul District (2) Yogyakarta District and (3) Cikampek District. CRD (Complete Random Design) was used with each population consisting of three replications (50 seeds/replication). The seed source showed significantly different results on the growth of S. cumini seedlings (height, diameter, number of leaves, wet weight of stem and leaves and dry weight of stem and leaves). However, the variation of seed sources did not significantly differ on survival, root wet mean, root dry weight, top-root ratio and seed quality index. Height, diameter, number of leaves, comparison starting from the greatest were Gunungkidul (30.42 cm / 2.93 mm / 21.42), Cikampek (24.64 cm / 2.82 mm / 21.06) and Yogyakarta (21.27 cm / 2.45 mm / 19.27). While The stem and leaves wet weight and stem and leaf biomass comparison from the highest were Gunungkidul (6.84 gram, 2.10 gram), Yogyakarta (4.95 gram / 1.59 gram) and Cikampek (4.24 gram / 1.47 gram). Moreover, seeds from the Gunungkidul population produced better growth than other populations. This study's recommendation is to develop rare plants of S. cumini species that will have the best growth by using seeds from the Gunungkidul","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87529712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1