Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.009
Mashudi, S. Pudjiono, D. Setiadi, N. Hidayati, M. Sulaeman, L. Baskorowati, R. Hendrati, M. Susanto
Swietenia macrophylla King. as known as wide leaf mahogany is a type of exotic species from Latin America that is common planted in Indonesia. This species is widely cultivated in several regions in Indonesia due to the high demand for carpentry timber. Therefore, to meet the high demand of wide leaf mahogany wood, the development of plantation forests of S. macrophylla with high productivity is very much needed. Development of plantation forests with high productivity requires the support of improvement seeds. Therefore, this study was conducted to determine the survival rate, growth variation between land race and between mother trees of 18 months old of S. macrophylla progeny trial. This progeny trial at the end of the selection can be converted into a seedling seed orchard (SSO) to produce the improvement seeds. Incomplete Block Design with two factors, i.e. land race (5 levels) and mother tree (50 levels) was used in this study. The results of the analysis showed that the survival rates of plant was significantly different between the land race but not among the mother trees. The average of survival rate of this species is only 71.58%, due to the landslide during the rainy season, burnt during the dry season and also exposed to stem borer attacks. Growth parameters (height and stem diameter) were significantly different between land race and mother tree. It showed that the Bondowoso land race exhibited the best performance of height and stem diameter, respectively 151.55 cm and 21.97 mm. The best mother tree in terms of growth (height and stem diameter) were exhibited on 19 individual tresses, with a range from 130.38 to 171.64 cm for height character and from 20.46 to 26.47 mm for stem diameter character.
大叶甜菊王。宽叶桃花心木是一种来自拉丁美洲的外来物种，在印度尼西亚很常见。由于对木工木材的高需求，该物种在印度尼西亚的几个地区被广泛种植。因此，为满足我国对宽叶红木木材的高需求，发展高产的大叶红木人工林是十分必要的。高生产力人工林的发展需要改良种子的支持。因此，本研究对18月龄大叶松子代试验的成活率、地种间和母树间的生长变化进行了研究。选择结束后的后代试验可转为育苗果园生产改良种子。本研究采用土地种族(5个等级)和母树(50个等级)两个因素的不完全块设计。分析结果表明，植株成活率在不同地种间存在显著差异，而母树间无显著差异。由于雨季易发生滑坡，旱季易发生焚烧，且易受茎蛀虫侵害，平均成活率仅为71.58%。陆地种与母树的生长参数(株高和茎粗)差异显著。结果表明，高和茎粗分别为151.55 cm和21.97 mm, Bondowoso陆地小种表现最好。株高和茎粗的最佳母树株数为19株，株高130.38 ~ 171.64 cm，茎粗20.46 ~ 26.47 mm。
{"title":"Growth Variation of Swietenia macrophylla King. Progeny Trial at 18 Months of Age in Trenggalek East Java","authors":"Mashudi, S. Pudjiono, D. Setiadi, N. Hidayati, M. Sulaeman, L. Baskorowati, R. Hendrati, M. Susanto","doi":"10.2991/absr.k.210621.009","DOIUrl":"https://doi.org/10.2991/absr.k.210621.009","url":null,"abstract":"Swietenia macrophylla King. as known as wide leaf mahogany is a type of exotic species from Latin America that is common planted in Indonesia. This species is widely cultivated in several regions in Indonesia due to the high demand for carpentry timber. Therefore, to meet the high demand of wide leaf mahogany wood, the development of plantation forests of S. macrophylla with high productivity is very much needed. Development of plantation forests with high productivity requires the support of improvement seeds. Therefore, this study was conducted to determine the survival rate, growth variation between land race and between mother trees of 18 months old of S. macrophylla progeny trial. This progeny trial at the end of the selection can be converted into a seedling seed orchard (SSO) to produce the improvement seeds. Incomplete Block Design with two factors, i.e. land race (5 levels) and mother tree (50 levels) was used in this study. The results of the analysis showed that the survival rates of plant was significantly different between the land race but not among the mother trees. The average of survival rate of this species is only 71.58%, due to the landslide during the rainy season, burnt during the dry season and also exposed to stem borer attacks. Growth parameters (height and stem diameter) were significantly different between land race and mother tree. It showed that the Bondowoso land race exhibited the best performance of height and stem diameter, respectively 151.55 cm and 21.97 mm. The best mother tree in terms of growth (height and stem diameter) were exhibited on 19 individual tresses, with a range from 130.38 to 171.64 cm for height character and from 20.46 to 26.47 mm for stem diameter character.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80294752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.026
Helmiyetti, Darmi, Rezan Okto Wesa
{"title":"The Species and Abundance of Subterranean Termites (Insecta: Isoptera) in the Area of University of Bengkulu","authors":"Helmiyetti, Darmi, Rezan Okto Wesa","doi":"10.2991/absr.k.210621.026","DOIUrl":"https://doi.org/10.2991/absr.k.210621.026","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89397484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.008
L. Baskorowati, R. Hendrati, N. Hidayati, Mashudi, M. Susanto, D. Setiadi
In anticipating the rapid decrease of energy resource, especially fossil fuel, immediate actions are required to fulfil the needs of highly increased population in the world. Innovation to produce renewable energy from easily utilized raw materials should meet society’s needs including renewable raw materials from wood biomass for energy. Genetically improved Calliandra calothyrsus has been developed for this purpose. Due to low success rate of its reproduction, study on its reproductive biology was undertaken. The study was carried out during the flowering seasons of C. calothyrsus, by examining flower morphology, pollen, and pistil viabilities as wee as insect visitors to this species’ flowers. The study showed that the flowers of C. calothyrsus are borne on inflorescences (spikes) and carrying an average of 354 individual flowers, which develop acropetally. Pistils (female organ) cease to develop by the 2 to 3 day after their appearance, and the flowering is becoming ceased then. Pistils showed maturity during the period of 09.00 WIB 10.00 WIB and pollen is still viable from 1 to 3 days after the pollen opening. Three most frequent visitors (families: Apidae, Vespidae, Formicidae) and one order lepidoptera were found visiting C. calothyrsus flowers However, the reproductive success of C. calotyrsus is found to be very low, which is only 0.024. These preliminary results confirmed that the low reproductive success of C. calothyrsus is due to the structure and development of flowers allowing the incidences of both outcrossing and self-pollination. The limitation time of pistil maturity might also contribute to the low reproductive success of this species.
{"title":"Low Reproduction Success of Calliandra calothyrsus and It’s Implication for Breeding","authors":"L. Baskorowati, R. Hendrati, N. Hidayati, Mashudi, M. Susanto, D. Setiadi","doi":"10.2991/absr.k.210621.008","DOIUrl":"https://doi.org/10.2991/absr.k.210621.008","url":null,"abstract":"In anticipating the rapid decrease of energy resource, especially fossil fuel, immediate actions are required to fulfil the needs of highly increased population in the world. Innovation to produce renewable energy from easily utilized raw materials should meet society’s needs including renewable raw materials from wood biomass for energy. Genetically improved Calliandra calothyrsus has been developed for this purpose. Due to low success rate of its reproduction, study on its reproductive biology was undertaken. The study was carried out during the flowering seasons of C. calothyrsus, by examining flower morphology, pollen, and pistil viabilities as wee as insect visitors to this species’ flowers. The study showed that the flowers of C. calothyrsus are borne on inflorescences (spikes) and carrying an average of 354 individual flowers, which develop acropetally. Pistils (female organ) cease to develop by the 2 to 3 day after their appearance, and the flowering is becoming ceased then. Pistils showed maturity during the period of 09.00 WIB 10.00 WIB and pollen is still viable from 1 to 3 days after the pollen opening. Three most frequent visitors (families: Apidae, Vespidae, Formicidae) and one order lepidoptera were found visiting C. calothyrsus flowers However, the reproductive success of C. calotyrsus is found to be very low, which is only 0.024. These preliminary results confirmed that the low reproductive success of C. calothyrsus is due to the structure and development of flowers allowing the incidences of both outcrossing and self-pollination. The limitation time of pistil maturity might also contribute to the low reproductive success of this species.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88726717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.075
Debie Rizqoh, Sipriyadi, Shella Sharon, I. Rachmawati, Wulan Okta Kumala, C. N. Putri
Phyllosphere bacteria were bacteria that inhabit around the leaf surface. Several studies showed the potential of phyllosphere bacteria in producing bioactive compounds, one of which is antimicrobial compounds. Andaliman (Zanthoxylum acanthopodium DC.) was a plant from the Rutaceae family that was commonly found in North Sumatra, and its fruit was widely used as a spice in traditional cooking by the Batak tribe. Several studies showed that the andaliman terpenoid has antioxidant and antimicrobial activity and an immunostimulating effect. This study aimed to explore the potential of andaliman phyllosphere bacteria. The research design method was a qualitative experimental model. The research stages used in compiling this paper consisted of phyllosphere bacteria isolation, phyllosphere bacteria morphological characterization, and Gram stain. This study succeeded in isolating 206 colonies of andaliman phyllosphere bacteria with various colony forms and cell morphology. From 64 samples of phyllosphere bacterial isolate observed, we found 25 different colony forms, and all samples were Gram-positive bacteria. Microscopic observations of gram staining showed that 44 isolates (68.75%) were cocci, 17 isolates (26.56%) were in the form of bacillus, and three isolates (4.68%) were in the form of coccobacillus.
{"title":"Exploration of Phyllosphere Bacteria From Andaliman (Zanthoxylum acanthopodium DC.)","authors":"Debie Rizqoh, Sipriyadi, Shella Sharon, I. Rachmawati, Wulan Okta Kumala, C. N. Putri","doi":"10.2991/absr.k.210621.075","DOIUrl":"https://doi.org/10.2991/absr.k.210621.075","url":null,"abstract":"Phyllosphere bacteria were bacteria that inhabit around the leaf surface. Several studies showed the potential of phyllosphere bacteria in producing bioactive compounds, one of which is antimicrobial compounds. Andaliman (Zanthoxylum acanthopodium DC.) was a plant from the Rutaceae family that was commonly found in North Sumatra, and its fruit was widely used as a spice in traditional cooking by the Batak tribe. Several studies showed that the andaliman terpenoid has antioxidant and antimicrobial activity and an immunostimulating effect. This study aimed to explore the potential of andaliman phyllosphere bacteria. The research design method was a qualitative experimental model. The research stages used in compiling this paper consisted of phyllosphere bacteria isolation, phyllosphere bacteria morphological characterization, and Gram stain. This study succeeded in isolating 206 colonies of andaliman phyllosphere bacteria with various colony forms and cell morphology. From 64 samples of phyllosphere bacterial isolate observed, we found 25 different colony forms, and all samples were Gram-positive bacteria. Microscopic observations of gram staining showed that 44 isolates (68.75%) were cocci, 17 isolates (26.56%) were in the form of bacillus, and three isolates (4.68%) were in the form of coccobacillus.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88826121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.047
Marindha Febriani, Abinawanto, R. R. Dewi
{"title":"Sperm Motility of Brek Fish (Systomus Orphoides) Using Soy Extract as a Supplementation Extender","authors":"Marindha Febriani, Abinawanto, R. R. Dewi","doi":"10.2991/absr.k.210621.047","DOIUrl":"https://doi.org/10.2991/absr.k.210621.047","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87806499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.050
Rifqi Ramadhana, Abinawanto, R. Gustiano
The purpose of this research was to evaluate the effect of skim milk on sperm motility of Brek fish, Systomus orphoides (Valenciennes, 1842) after 48 hours in 4 ̊C. The method in this research is sperm was collected by hand stripping and was diluted by diluent, which consisted of the fish ringer, skim milk and methanol 10%. The ratio of sperm to diluent is 1:9. The concentrations of skim milk used in this research were 0%, 5%, 10%, 15%, and 20%, respectively. The sperm was stored at 4 ̊C for 48 hours after diluted sperm is thawed in room temperate for 1 minute and sperm motility was observed in improved Neubauer with a light microscope.The motility percentage was analyzed using ANOVA and Tukey test. According to the Annova test (P>0.05), 10% of skim milk showed the highest sperm motility (92.18 + 3.05) % after five replication per dose. Ten percent of skim milk was the optimum concentration to preserve sperm motility at 4 ̊C for 48 hours compared to 0%, 5%, 15%, and 20%. The result of each concentration is 90,85%, 84,68%, 79,54%, and 74,14% respectively.
{"title":"Effect of Skim Milk on Sperm Motility of Brek Fish, Systomus orphoides (Valenciennes, 1842) for Short Term Preservation","authors":"Rifqi Ramadhana, Abinawanto, R. Gustiano","doi":"10.2991/absr.k.210621.050","DOIUrl":"https://doi.org/10.2991/absr.k.210621.050","url":null,"abstract":"The purpose of this research was to evaluate the effect of skim milk on sperm motility of Brek fish, Systomus orphoides (Valenciennes, 1842) after 48 hours in 4 ̊C. The method in this research is sperm was collected by hand stripping and was diluted by diluent, which consisted of the fish ringer, skim milk and methanol 10%. The ratio of sperm to diluent is 1:9. The concentrations of skim milk used in this research were 0%, 5%, 10%, 15%, and 20%, respectively. The sperm was stored at 4 ̊C for 48 hours after diluted sperm is thawed in room temperate for 1 minute and sperm motility was observed in improved Neubauer with a light microscope.The motility percentage was analyzed using ANOVA and Tukey test. According to the Annova test (P>0.05), 10% of skim milk showed the highest sperm motility (92.18 + 3.05) % after five replication per dose. Ten percent of skim milk was the optimum concentration to preserve sperm motility at 4 ̊C for 48 hours compared to 0%, 5%, 15%, and 20%. The result of each concentration is 90,85%, 84,68%, 79,54%, and 74,14% respectively.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82901308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.092
W. Darwis, R. H. Wibowo, Sipriyadi, Raden Roro Upiek Ngesti Wibawaning Astuti, Lam Way Sitorus
White oyster mushroom (Pleurotus ostreatus) is a type of fungus wood that has a high nutritional content. White oyster mushroom cultivation begins with the provision of good quality seeds which include 4 stages, namely F1 seeds, F2, F3 and baglog. This study aims to influence the composition of the green bean, maize and rice grain media composition on the growth of mycelium F2 parent white oyster mushroom. This research was conducted at the Microbiology Laboratory, Department of Biology. The research design used a randomized block design (RBD), with the parameters of observing the propagation length, color and thickness of the mycelium. The results showed that the composition of different media had a significant effect on the propagation of mycelium in P2, P9, P10, P18, P19, P20 with the highest length of 14 cm, compact white color and evenly thick on combination media. Environmental conditions suitable for the growth of mycelium seeds F2 white oyster mushroom include temperature 27-28 ̊C, humidity 60-62%, and light intensity 24-54 Lux.
{"title":"The Influence of Green, Corn, and Rice Seed Media Composition on Mycelium Growth of F2 Main Seeds (Pleurotus ostreatus (Jacq. Ex Fr) Kummer)","authors":"W. Darwis, R. H. Wibowo, Sipriyadi, Raden Roro Upiek Ngesti Wibawaning Astuti, Lam Way Sitorus","doi":"10.2991/absr.k.210621.092","DOIUrl":"https://doi.org/10.2991/absr.k.210621.092","url":null,"abstract":"White oyster mushroom (Pleurotus ostreatus) is a type of fungus wood that has a high nutritional content. White oyster mushroom cultivation begins with the provision of good quality seeds which include 4 stages, namely F1 seeds, F2, F3 and baglog. This study aims to influence the composition of the green bean, maize and rice grain media composition on the growth of mycelium F2 parent white oyster mushroom. This research was conducted at the Microbiology Laboratory, Department of Biology. The research design used a randomized block design (RBD), with the parameters of observing the propagation length, color and thickness of the mycelium. The results showed that the composition of different media had a significant effect on the propagation of mycelium in P2, P9, P10, P18, P19, P20 with the highest length of 14 cm, compact white color and evenly thick on combination media. Environmental conditions suitable for the growth of mycelium seeds F2 white oyster mushroom include temperature 27-28 ̊C, humidity 60-62%, and light intensity 24-54 Lux.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87739766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.065
R. Agus, I. I. Fidhatami, M. Hatta
{"title":"Cloning and Expression of Recombinant Protein MPT63 of Mycobacterium tuberculosis Indonesian Isolate as Serodiagnostic Latent Tuberculosis","authors":"R. Agus, I. I. Fidhatami, M. Hatta","doi":"10.2991/absr.k.210621.065","DOIUrl":"https://doi.org/10.2991/absr.k.210621.065","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78274444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.090
Vilya Syafriana, Erwi Putri Setyaningsih, Nabilah Rachmawani, D. Kharisma, F. Hamida
{"title":"Antimicrobial Activity of Ethanol Extract of Akar Kaik-kaik (Uncaria cordata (Lour.) Merr.) Leaves Against Staphylococcus aureus, Escherichia coli, and Candida albicans","authors":"Vilya Syafriana, Erwi Putri Setyaningsih, Nabilah Rachmawani, D. Kharisma, F. Hamida","doi":"10.2991/absr.k.210621.090","DOIUrl":"https://doi.org/10.2991/absr.k.210621.090","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82230679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.2991/absr.k.210621.046
Junaidi, Atminingsih, M. Andriyanto
The bud-grafting method is the most economical technique of Hevea brasiliensis propagation. This technique requires rootstock and scion. The high rootstock demand for the replanting program drives the seeds’ suppliers to collect assorted seeds from cultivated fields, lead to a high variation in the germination rate. The time from seed dehiscent to seeding was hypothesized to be crucial inflicting an inconsistency in the germination rate and seedling rootstock growth. This present study used rubber seeds of the GT1 clone which were laid under trees’ shading to mimic the natural environment. Five laying periods were applied i.e., P0 (directly germinated, control), P1 (laid for one week), P2 (laid for two weeks), P3 (laid for three weeks), and P4 (laid for four weeks). Our study implied that the direct sowing after dehiscent was the best way to attain a high germination rate (GR) (51.67%) while delaying seed sowing for one week reduced the GR up to 22.04%. The longer delaying in P2, P3, and P4 did not show significant differences among them which were 13.33%, 1.19%, and 4.35% respectively. However, the seedling showed a similar growth rate including root/shoot ratio regardless of the laying period treatment. The necessity of establishing seed gardens for rootstock was highlighted in which the harvesting of the seeds could be controlled.
{"title":"Seed Collection Time Effect on the Germination Rate and Growth of Rubber Tree Rootstock","authors":"Junaidi, Atminingsih, M. Andriyanto","doi":"10.2991/absr.k.210621.046","DOIUrl":"https://doi.org/10.2991/absr.k.210621.046","url":null,"abstract":"The bud-grafting method is the most economical technique of Hevea brasiliensis propagation. This technique requires rootstock and scion. The high rootstock demand for the replanting program drives the seeds’ suppliers to collect assorted seeds from cultivated fields, lead to a high variation in the germination rate. The time from seed dehiscent to seeding was hypothesized to be crucial inflicting an inconsistency in the germination rate and seedling rootstock growth. This present study used rubber seeds of the GT1 clone which were laid under trees’ shading to mimic the natural environment. Five laying periods were applied i.e., P0 (directly germinated, control), P1 (laid for one week), P2 (laid for two weeks), P3 (laid for three weeks), and P4 (laid for four weeks). Our study implied that the direct sowing after dehiscent was the best way to attain a high germination rate (GR) (51.67%) while delaying seed sowing for one week reduced the GR up to 22.04%. The longer delaying in P2, P3, and P4 did not show significant differences among them which were 13.33%, 1.19%, and 4.35% respectively. However, the seedling showed a similar growth rate including root/shoot ratio regardless of the laying period treatment. The necessity of establishing seed gardens for rootstock was highlighted in which the harvesting of the seeds could be controlled.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81924807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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