M. Malboobi, Katayoun Zamani, T. Lohrasebi, M. Sarikhani, Ali Samaian, M. S. Sabet
Phosphorus (P) is one of the most vital elements for all living organisms which acts as aconstituent of essential biomolecules such as nucleic acids, phospholipids, and phosphosugars,and as a major contributor to almost all metabolic reactions including photosynthesis,respiration, and energy delivery. It is one of the most needed nutrients for plant growth anddevelopment. Despite high levels of P in the soil, plants absorb it only in the soluble inorganicform of free phosphate ion (Pi) which is scarce in soil. Therefore, there has been a large demandfor Pi fertilizers to secure crop yields, yet its deposition in soil and gradual run-off into waterreservoirs lead to chains of events that cause irreversible damages to ecosystems. Researches,including genome-wide data analyses, have revealed interesting molecular aspects of plantadaptive strategies to deal with low Pi concentrations in soil. These include the higherexpression of acid phosphatases and Pi transporters as well as the secretion of organic acids inthe rhizosphere that maintain cellular Pi homeostasis in order to keep metabolic reactionsrunning. Describing the cycle of Pi exchange between physical and biological worlds, the extentto which current agricultural practices are disturbing the cycle, the necessity of introducing lessdestructivemethods of providing Pi, and alternative measures and solutions for sustainableagriculture will be discussed in this review.
{"title":"Phosphate: the Silent Challenge","authors":"M. Malboobi, Katayoun Zamani, T. Lohrasebi, M. Sarikhani, Ali Samaian, M. S. Sabet","doi":"10.22059/PBS.2014.50302","DOIUrl":"https://doi.org/10.22059/PBS.2014.50302","url":null,"abstract":"Phosphorus (P) is one of the most vital elements for all living organisms which acts as aconstituent of essential biomolecules such as nucleic acids, phospholipids, and phosphosugars,and as a major contributor to almost all metabolic reactions including photosynthesis,respiration, and energy delivery. It is one of the most needed nutrients for plant growth anddevelopment. Despite high levels of P in the soil, plants absorb it only in the soluble inorganicform of free phosphate ion (Pi) which is scarce in soil. Therefore, there has been a large demandfor Pi fertilizers to secure crop yields, yet its deposition in soil and gradual run-off into waterreservoirs lead to chains of events that cause irreversible damages to ecosystems. Researches,including genome-wide data analyses, have revealed interesting molecular aspects of plantadaptive strategies to deal with low Pi concentrations in soil. These include the higherexpression of acid phosphatases and Pi transporters as well as the secretion of organic acids inthe rhizosphere that maintain cellular Pi homeostasis in order to keep metabolic reactionsrunning. Describing the cycle of Pi exchange between physical and biological worlds, the extentto which current agricultural practices are disturbing the cycle, the necessity of introducing lessdestructivemethods of providing Pi, and alternative measures and solutions for sustainableagriculture will be discussed in this review.","PeriodicalId":20726,"journal":{"name":"Progress in Biological Sciences","volume":"201 1","pages":"1-32"},"PeriodicalIF":0.0,"publicationDate":"2014-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80160693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microalgal growth curve, after the exponential phase, shows a stationary phase where algalbiomass production is inhibited and remains constant by some factors such as nutrientdepletion and intrinsic behavior. The present study is concerned with evaluating effects ofthe culture medium condition and intrinsic behavior on biomass production by Dunaliellatertiolecta. To this end, effect of pH, nutrient concentration, CO2 (NaHCO3) concentration,possible secreted substances, and cell density on the biomass production by D. tertiolectawere investigated. The results showed that biomass yield can be significantly affected by pH(p<0.01) and nutrient (p<0.05). In a combination of pH and nutrient, the biomass was found tobe more influenced by the pH, compared with the nutrients. The results showed a significantinteraction between nutrient and NaHCO3 (p<0.05), suggesting that CO2 concentration maylimit biomass production only when sufficient nutrients are available. Nutrients concentrationand biomass production showed a direct correlation (p<0.05). The rate of reaching themaximum biomass was showed to be increased in higher nutrients concentration; however,the maximum point could not to be affected. The existence of secreted compounds withinhibitory effect on the growth was not observed. The inhibitory effect of the cell density onbiomass production can not be confirmed.
{"title":"Study of the inhibitory effect of the media culture parameters and cell population to increase the biomass production of Dunaliella tertiolecta","authors":"R. Taheri, M. Shariati","doi":"10.22059/PBS.2013.35829","DOIUrl":"https://doi.org/10.22059/PBS.2013.35829","url":null,"abstract":"Microalgal growth curve, after the exponential phase, shows a stationary phase where algalbiomass production is inhibited and remains constant by some factors such as nutrientdepletion and intrinsic behavior. The present study is concerned with evaluating effects ofthe culture medium condition and intrinsic behavior on biomass production by Dunaliellatertiolecta. To this end, effect of pH, nutrient concentration, CO2 (NaHCO3) concentration,possible secreted substances, and cell density on the biomass production by D. tertiolectawere investigated. The results showed that biomass yield can be significantly affected by pH(p<0.01) and nutrient (p<0.05). In a combination of pH and nutrient, the biomass was found tobe more influenced by the pH, compared with the nutrients. The results showed a significantinteraction between nutrient and NaHCO3 (p<0.05), suggesting that CO2 concentration maylimit biomass production only when sufficient nutrients are available. Nutrients concentrationand biomass production showed a direct correlation (p<0.05). The rate of reaching themaximum biomass was showed to be increased in higher nutrients concentration; however,the maximum point could not to be affected. The existence of secreted compounds withinhibitory effect on the growth was not observed. The inhibitory effect of the cell density onbiomass production can not be confirmed.","PeriodicalId":20726,"journal":{"name":"Progress in Biological Sciences","volume":"90 1","pages":"123-133"},"PeriodicalIF":0.0,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83914828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. A. Pormehr, M. Pourkazemi, M. Yarmohammadi, Yalda Banismaeili, M. Sanati
In this study, the cDNA Growth Hormone (cGH) of the Belugasturgeon (Husohuso) and Russian sturgeon (Acipensergueldenstaedtii) were cloned and sequenced, and phylogenetic relationships were examined using nucleic acid and amino acid sequences. The nucleotide sequence of the Beluga GH has an open reading frame of 645 nucleotides encoding a protein 214 amino acid residues. The signal peptide cleavage site was predicted to be at position 72, yeilding a signal peptide of 24aminoacid residues and a mature peptide of 190 amino acids. The cDNAsequence of the Russian sturgeon was similar to that of the Beluga cGH. The phylogenetic analysis was performed based on amino acid and DNA sequences using the neighbor oining(NJ) and Maximum parsimony (MP) method. Phylogenic trees by the two methods wereidentical in most of the clades with the high bootstrap support, and the topology of amino acid and DNA sequences showed highest similarity with mammalian sequences.
{"title":"Phylogenetic and sequence analysis of the growth hormone gene of two sturgeons, Huso huso and Acipenser Gueldenstaedtii","authors":"L. A. Pormehr, M. Pourkazemi, M. Yarmohammadi, Yalda Banismaeili, M. Sanati","doi":"10.22059/PBS.2013.35827","DOIUrl":"https://doi.org/10.22059/PBS.2013.35827","url":null,"abstract":"In this study, the cDNA Growth Hormone (cGH) of the Belugasturgeon (Husohuso) and Russian sturgeon (Acipensergueldenstaedtii) were cloned and sequenced, and phylogenetic relationships were examined using nucleic acid and amino acid sequences. The nucleotide sequence of the Beluga GH has an open reading frame of 645 nucleotides encoding a protein 214 amino acid residues. The signal peptide cleavage site was predicted to be at position 72, yeilding a signal peptide of 24aminoacid residues and a mature peptide of 190 amino acids. The cDNAsequence of the Russian sturgeon was similar to that of the Beluga cGH. The phylogenetic analysis was performed based on amino acid and DNA sequences using the neighbor oining(NJ) and Maximum parsimony (MP) method. Phylogenic trees by the two methods wereidentical in most of the clades with the high bootstrap support, and the topology of amino acid and DNA sequences showed highest similarity with mammalian sequences.","PeriodicalId":20726,"journal":{"name":"Progress in Biological Sciences","volume":"44 1","pages":"99-105"},"PeriodicalIF":0.0,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91232670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Effects of excess concentrations of boron on major cell wall components of tobacco cells(Nicotiana tabacum L. cv. Burley 21) were studied. Pectin, xyloglucan, hydroxyproline-richglycoproteins (extensin), and arabinogalactan proteins were characterized. Results showedthat increased boron supply resulted in significant decrease in cell and cell wall dry weights.Also, high concentrations of boron reduced relative amounts of major wall components.Compared with the normal conditions, increase in certain sugars in pectin (e.g., xylose andits methylated derivative) and decrease in glucose, arabinose, and rhamnose in those treatedcells with higher concentrations of boron was significant. The content of hydroxyprolinerichglycoproteins decreased when tobacco cells were supplied with higher concentrations ofboron. In these cells, increased ratio of protein to glycan part and increase in hydroxyl lysineamong other amino acids were noticeable. Amount of arabinogalactan proteins also decreasedas boron supply increased. Excess boron did not alter the composition and concentration ofamino acids of arabinogalactan; however, it remarkably increased xylose and glucose butdecreased galactose and arabinose concentrations.
{"title":"Changes of major wall polysaccharides and glycoproteins of tobacco cells in response to excess boron","authors":"F. Ghanati, M. D. Heidarabadi","doi":"10.22059/PBS.2013.35841","DOIUrl":"https://doi.org/10.22059/PBS.2013.35841","url":null,"abstract":"Effects of excess concentrations of boron on major cell wall components of tobacco cells(Nicotiana tabacum L. cv. Burley 21) were studied. Pectin, xyloglucan, hydroxyproline-richglycoproteins (extensin), and arabinogalactan proteins were characterized. Results showedthat increased boron supply resulted in significant decrease in cell and cell wall dry weights.Also, high concentrations of boron reduced relative amounts of major wall components.Compared with the normal conditions, increase in certain sugars in pectin (e.g., xylose andits methylated derivative) and decrease in glucose, arabinose, and rhamnose in those treatedcells with higher concentrations of boron was significant. The content of hydroxyprolinerichglycoproteins decreased when tobacco cells were supplied with higher concentrations ofboron. In these cells, increased ratio of protein to glycan part and increase in hydroxyl lysineamong other amino acids were noticeable. Amount of arabinogalactan proteins also decreasedas boron supply increased. Excess boron did not alter the composition and concentration ofamino acids of arabinogalactan; however, it remarkably increased xylose and glucose butdecreased galactose and arabinose concentrations.","PeriodicalId":20726,"journal":{"name":"Progress in Biological Sciences","volume":"19 1","pages":"27-38"},"PeriodicalIF":0.0,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74465508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Mirmasoumi, P. Azadi, A. Sharafi, Valentine Ntui Otang, M. Mii
Transverse thin cell layer sections excised from in vitro scales of Lilium ledebourii were cultured on Murashige and Skoog (MS) medium supplemented with various plant growth regulators (PGRs) at different concentrations. Although, bulblets were produced on PGRfree MS medium during organogenesis, addition of 0.25 mg l-1 6-benzyladenine or 5.0 mg l-1 indole-3-acetic acid to the medium increased the organogenesis response and produced 4.4 bulblets per explants. Two months later, the bulblets were transferred to MS PGRs-free medium. Bulblets were successfully transplanted to the soil after a cold treatment of 8 weeks,with a survival rate of 85%.
{"title":"Simple protocol for plant regeneration of Lilium ledebourii using transverse thin cell layer","authors":"M. Mirmasoumi, P. Azadi, A. Sharafi, Valentine Ntui Otang, M. Mii","doi":"10.22059/PBS.2013.35828","DOIUrl":"https://doi.org/10.22059/PBS.2013.35828","url":null,"abstract":"Transverse thin cell layer sections excised from in vitro scales of Lilium ledebourii were cultured on Murashige and Skoog (MS) medium supplemented with various plant growth regulators (PGRs) at different concentrations. Although, bulblets were produced on PGRfree MS medium during organogenesis, addition of 0.25 mg l-1 6-benzyladenine or 5.0 mg l-1 indole-3-acetic acid to the medium increased the organogenesis response and produced 4.4 bulblets per explants. Two months later, the bulblets were transferred to MS PGRs-free medium. Bulblets were successfully transplanted to the soil after a cold treatment of 8 weeks,with a survival rate of 85%.","PeriodicalId":20726,"journal":{"name":"Progress in Biological Sciences","volume":"58 1","pages":"117-122"},"PeriodicalIF":0.0,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90222265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pyricularia oryzae (Tel. Magnaporthegrisea) is currently used as a fungal model for plantmicrobeinteraction studies as well as an indicative model for anticancer drug discovery. Thepresent study introduces the optimal condition in which P. oryzae grows and sporulates beston common culture media. We have considered three fungal culture media, i.e. PDA, PCA andWA, based on which P. oryzae sporulation inducers like rice polish, rice extract or rice leafsegments could be added, and evaluated both for vegetative growth and sporulation. Threelight regimens, i.e. continuous light, 16.8 hr light/darkness, and continuous darkness wereapplied in combination with nine synthetic culture media. Mycelial growth was measuredafter 11 days, but sporulation was tracked on the 10th, 20th, and 30th day after incubation at26oC. The findings indicate that PDA culture medium could provide the best medium for P.oryzae vegetative growth, regardless of light condition. However, P. oryzae could sporulatewhen light was provided either continuously or at intervals. A combination of 16.8 hr light/darkness intervals and adding rice materials to culture media could induce P. oryzae for abetter sporulation. RPCA can be used as the best culture medium for P. oryzae in order toobtain a high number of conidia under light alterations. Moreover, aging increases the totalnumber of conidia.
{"title":"An investigation on the effects of photoperiod, aging and culture media onvegetative growth and sporulation of rice blast pathogen pyricularia oryzae","authors":"Mahdieh S. Hosseyni-Moghaddam, J. Soltani","doi":"10.22059/PBS.2013.35843","DOIUrl":"https://doi.org/10.22059/PBS.2013.35843","url":null,"abstract":"Pyricularia oryzae (Tel. Magnaporthegrisea) is currently used as a fungal model for plantmicrobeinteraction studies as well as an indicative model for anticancer drug discovery. Thepresent study introduces the optimal condition in which P. oryzae grows and sporulates beston common culture media. We have considered three fungal culture media, i.e. PDA, PCA andWA, based on which P. oryzae sporulation inducers like rice polish, rice extract or rice leafsegments could be added, and evaluated both for vegetative growth and sporulation. Threelight regimens, i.e. continuous light, 16.8 hr light/darkness, and continuous darkness wereapplied in combination with nine synthetic culture media. Mycelial growth was measuredafter 11 days, but sporulation was tracked on the 10th, 20th, and 30th day after incubation at26oC. The findings indicate that PDA culture medium could provide the best medium for P.oryzae vegetative growth, regardless of light condition. However, P. oryzae could sporulatewhen light was provided either continuously or at intervals. A combination of 16.8 hr light/darkness intervals and adding rice materials to culture media could induce P. oryzae for abetter sporulation. RPCA can be used as the best culture medium for P. oryzae in order toobtain a high number of conidia under light alterations. Moreover, aging increases the totalnumber of conidia.","PeriodicalId":20726,"journal":{"name":"Progress in Biological Sciences","volume":"18 1","pages":"135-143"},"PeriodicalIF":0.0,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74366547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Nazari, M. Pourkazemi, M. Khoshkholgh, Leila Azizzade
Mitochondria1 DNA (mtDNA) control region sequences were analyzed to evaluate the population genetic structure of Persian sturgeon (Acipenser persicus) in Caspian Sea. A total of 45 specimens were collected from the different locations of the Caspian Sea. MtDNA control region was amplified using PCR. Direct sequencing was performed according standard method. The results showed that 12 haplotypes were observed between 45 samples in the method. The highest numbers of haplotypes were observed in Sefidroud River in which 3 haplotypes A, B and E among them were specific for the river and were not observed in the other locations. The average haplotype diversity (h) and nucleotide diversity (π) were 0.795±0.037 and 0.0062±0.0046, respectively. The results of FST based on kimura- 2 parameters method and analysis of molecular variance (AMOVA) demonstrated that most variations occurred between samples from Sefidroud River in the south Caspian Sea and that the samples include three distinct populations including Sefidrud, Russia and Azerbaijan (P<0.001). As mtDNA control region is hypervariable segment, this can be provide potential marker for identifying probable populations and for determining their management and conservation units, leading to the useful application of molecular genetics in investigating conservation biology of the Persian sturgeon.
{"title":"Population structure and variation in Persian sturgeon (Acipenser percicus ) from the Caspian Sea as determind from mitochondrial DNA sequences of the control region","authors":"S. Nazari, M. Pourkazemi, M. Khoshkholgh, Leila Azizzade","doi":"10.22059/PBS.2013.35826","DOIUrl":"https://doi.org/10.22059/PBS.2013.35826","url":null,"abstract":"Mitochondria1 DNA (mtDNA) control region sequences were analyzed to evaluate the population genetic structure of Persian sturgeon (Acipenser persicus) in Caspian Sea. A total of 45 specimens were collected from the different locations of the Caspian Sea. MtDNA control region was amplified using PCR. Direct sequencing was performed according standard method. The results showed that 12 haplotypes were observed between 45 samples in the method. The highest numbers of haplotypes were observed in Sefidroud River in which 3 haplotypes A, B and E among them were specific for the river and were not observed in the other locations. The average haplotype diversity (h) and nucleotide diversity (π) were 0.795±0.037 and 0.0062±0.0046, respectively. The results of FST based on kimura- 2 parameters method and analysis of molecular variance (AMOVA) demonstrated that most variations occurred between samples from Sefidroud River in the south Caspian Sea and that the samples include three distinct populations including Sefidrud, Russia and Azerbaijan (P<0.001). As mtDNA control region is hypervariable segment, this can be provide potential marker for identifying probable populations and for determining their management and conservation units, leading to the useful application of molecular genetics in investigating conservation biology of the Persian sturgeon.","PeriodicalId":20726,"journal":{"name":"Progress in Biological Sciences","volume":"42 1","pages":"67-80"},"PeriodicalIF":0.0,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80602636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
There is a great call for using microbial bio-decaffeination approach to remove caffeinefrom caffeinated products and industrial wastes. We aimed in this study to screen strainsof yeasts which exhibit high caffeine tolerance and to investigate the bio-degradation ofcaffeine under growth conditions. Sixteen yeast strains were isolated from the cultivatedtea soils collected from sites of northern Iran and evaluated for the caffeine tolerance bythe agar dilution method. Based on the tolerance efficiency, strain TFS9 was selected andidentified as Saccharomyces cerevisiae TFS9 (GenBank accession number KF414526)on the morphological and bioochemical characteristics as well as molecular phylogeneticstudies based on amplification the ITS1–5.8S–ITS2 rDNA sequences. The time course ofcaffeine removal by growing cells of the strain TFS9 in the minimal salt medium containingcaffeine as the sole source of carbon was estimated by a decrease in caffeine absorbanceusing UV-visible spectrophotometer. The concentration of caffeine in the supernatantof the yeast culture medium decreased by 84.8% (from 3.5g/l to 0.53 g/l) after 60h ofincubation by using of S. cerevisiae TFS9, without additional optimization process. Resultsof experimental studies suggest a simple and cost-effective process for the microbialdecaffeination of caffeine-containing solutions, and provide a promising approach fordeveloping safe processes that can be used effectively for decaffeination of industrialeffluents. The present study provides the first evidence on the caffeine bio-degradationusing yeast species of S. cerevisiae.
{"title":"Saccharomyces cerevisiae TFS9, a novel isolated yeast capable of high caffeine-tolerant and its application in biodecaffeination approach","authors":"M. Ashengroph, M. Borchaluei","doi":"10.22059/PBS.2013.35844","DOIUrl":"https://doi.org/10.22059/PBS.2013.35844","url":null,"abstract":"There is a great call for using microbial bio-decaffeination approach to remove caffeinefrom caffeinated products and industrial wastes. We aimed in this study to screen strainsof yeasts which exhibit high caffeine tolerance and to investigate the bio-degradation ofcaffeine under growth conditions. Sixteen yeast strains were isolated from the cultivatedtea soils collected from sites of northern Iran and evaluated for the caffeine tolerance bythe agar dilution method. Based on the tolerance efficiency, strain TFS9 was selected andidentified as Saccharomyces cerevisiae TFS9 (GenBank accession number KF414526)on the morphological and bioochemical characteristics as well as molecular phylogeneticstudies based on amplification the ITS1–5.8S–ITS2 rDNA sequences. The time course ofcaffeine removal by growing cells of the strain TFS9 in the minimal salt medium containingcaffeine as the sole source of carbon was estimated by a decrease in caffeine absorbanceusing UV-visible spectrophotometer. The concentration of caffeine in the supernatantof the yeast culture medium decreased by 84.8% (from 3.5g/l to 0.53 g/l) after 60h ofincubation by using of S. cerevisiae TFS9, without additional optimization process. Resultsof experimental studies suggest a simple and cost-effective process for the microbialdecaffeination of caffeine-containing solutions, and provide a promising approach fordeveloping safe processes that can be used effectively for decaffeination of industrialeffluents. The present study provides the first evidence on the caffeine bio-degradationusing yeast species of S. cerevisiae.","PeriodicalId":20726,"journal":{"name":"Progress in Biological Sciences","volume":"70 1","pages":"145-156"},"PeriodicalIF":0.0,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86270223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Shahbazi, R. Kasra-Kermanshahi, S. Gharavi, Z. M. Nejad, Faezeh Borzooee
The anionic surfactant sodium dodecyl sulfate (SDS) was degraded by novel strain ofPseudomonas aeruginosa KGS under accession No. JQ328193, which was isolated from carwash wastewater. The purpose of this research was to study different optimization conditionsrequired for enhancing the biodegradation of sodium dodecyl sulfate P. aeruginosa KGS.Influence of different Physicochemical factors such as nitrogen and carbon sources, pH,temperature, inoculation percent and different concentrations of SDS on the biodegradation ofSDS were investigated by measuring the degradation rate of SDS using methylene blue activesubstance (MBAS) method. The optimum conditions determined for the this selected bacteriumstrain for degradation of SDS were 1.5mM SDS, inoculation percent 7%, pH 7.5, temperature37°C, ammonium nitrate (nitrogen source) when basal salt medium was supplemented withglucose as a co substrate. This bacterium is able to degrade about 98% of the SDS after 24h ofincubation under optimized conditions of biodegradation. The results presented in this researchindicate that Pseudomonas aeruginosa is a suitable candidate for SDS biodegradation.
{"title":"Optimal conditions for enhancing sodium dodecyl sulfate biodegradation by Pseudomonas aeruginosa KGS","authors":"R. Shahbazi, R. Kasra-Kermanshahi, S. Gharavi, Z. M. Nejad, Faezeh Borzooee","doi":"10.22059/PBS.2013.35840","DOIUrl":"https://doi.org/10.22059/PBS.2013.35840","url":null,"abstract":"The anionic surfactant sodium dodecyl sulfate (SDS) was degraded by novel strain ofPseudomonas aeruginosa KGS under accession No. JQ328193, which was isolated from carwash wastewater. The purpose of this research was to study different optimization conditionsrequired for enhancing the biodegradation of sodium dodecyl sulfate P. aeruginosa KGS.Influence of different Physicochemical factors such as nitrogen and carbon sources, pH,temperature, inoculation percent and different concentrations of SDS on the biodegradation ofSDS were investigated by measuring the degradation rate of SDS using methylene blue activesubstance (MBAS) method. The optimum conditions determined for the this selected bacteriumstrain for degradation of SDS were 1.5mM SDS, inoculation percent 7%, pH 7.5, temperature37°C, ammonium nitrate (nitrogen source) when basal salt medium was supplemented withglucose as a co substrate. This bacterium is able to degrade about 98% of the SDS after 24h ofincubation under optimized conditions of biodegradation. The results presented in this researchindicate that Pseudomonas aeruginosa is a suitable candidate for SDS biodegradation.","PeriodicalId":20726,"journal":{"name":"Progress in Biological Sciences","volume":"40 1","pages":"107-115"},"PeriodicalIF":0.0,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84964675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The identification of the efficiency of some mtDNA genes of Mus musculus species complex (house mouse) for biosystematics research was studied in this approach. Recent studies have made use of different mitochondrial genes including NADH dehydrogenase genes, cytochrome b gene, cytochrome oxidase genes, D-loop region and whole mtDNA genome to study the house mouse species. Usage of each of these genome regions has its own advantages and disadvantages. Identification of appropriate genomic regions is very important for molecular biosystematic research. We have shown here that NADH dehydrogenase and Cytochrome oxidase genes (particularly COX2) are especially useful in biosystematics studies and subspecies identification,whereas D-loop region is the best candidate for biogeographic and phylogeographic studiesof subspecies of this species efficiency-wise as well as economically. These candidates areintroduced considering that the first two gene complexes are highly conserved whereas the latteris well receptive to gaining and preserving the mutations through time.
{"title":"Evaluation of effectiveness of some mitochondrial genes in biosystematics and phylogeographic studies of house mouse (Mus musculus ) subspecies","authors":"Ahmadreza Katouzian, H. Rajabi-Maham","doi":"10.22059/PBS.2013.35825","DOIUrl":"https://doi.org/10.22059/PBS.2013.35825","url":null,"abstract":"The identification of the efficiency of some mtDNA genes of Mus musculus species complex (house mouse) for biosystematics research was studied in this approach. Recent studies have made use of different mitochondrial genes including NADH dehydrogenase genes, cytochrome b gene, cytochrome oxidase genes, D-loop region and whole mtDNA genome to study the house mouse species. Usage of each of these genome regions has its own advantages and disadvantages. Identification of appropriate genomic regions is very important for molecular biosystematic research. We have shown here that NADH dehydrogenase and Cytochrome oxidase genes (particularly COX2) are especially useful in biosystematics studies and subspecies identification,whereas D-loop region is the best candidate for biogeographic and phylogeographic studiesof subspecies of this species efficiency-wise as well as economically. These candidates areintroduced considering that the first two gene complexes are highly conserved whereas the latteris well receptive to gaining and preserving the mutations through time.","PeriodicalId":20726,"journal":{"name":"Progress in Biological Sciences","volume":"9 1","pages":"39-66"},"PeriodicalIF":0.0,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88147180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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