Protoplasma最新文献

Wild pomegranate is a potent medicinal plant known for its medicinal and nutritional attributes. Despite its healing and curative properties, the genome of this wild species remains elusive, thus limiting our understanding on the genetic processes involved in the biosynthesis of functional molecules. This study presents the annotation of a de novo genome assembly of wild pomegranate, with a genome size of 279.0 Mb. From the assembly, 34.8 GB of the data was retained, encompassing 72,055 scaffolds. A total of 49,178 genes were predicted, with an average of 5.36 exons per gene and a GC content of 49%. About 14,400 genes were annotated in biological, cellular and molecular processes related mostly to carbohydrate metabolism, intracellular signal transduction, mRNA binding and DNA helicase activity. KEGG enrichment analysis revealed maximum number of genes associated with biosynthesis of secondary metabolites mainly phenypropanoid pathway, followed by ribosome and plant hormone signal transduction. From the identified functional genes, 230 genes scaffolds encoded for transcription factors belonging to 25 families with highest recorded for MYB gene family. Study of annotated transposable elements unveiled the existence of long terminal repeats and retrotransposons. Additionally, our investigation involves the comparative analysis and identification of orthologous genes among the genomes of wild and cultivated species of Punica granatum and also across selected five plant species Eucalyptus grandis, Vitis vinifera, Jatropha curcas, Theobroma cacao and Gossypium raimondii, revealing the functional and evolutionary dynamics across species. To the best of our knowledge, this is the first report on the genome assembly, annotation and gene prediction in wild pomegranate. Also, information regarding the terpenoid pathway genes has been unravelled for the first time in the present study. Inclusively, the current study offers thorough details on important aspects of the wild pomegranate genome that would be useful in comprehending its genetics and will facilitate discovery of genes against various biotic and abiotic stresses.

Powdery mildew caused by Leveillula taurica adversely affects the development and growth of pepper plants. However, there have been few reports on the fine mapping and quantitative trait locus (QTLs) gene cloning of resistance genes to powdery mildew in pepper. Herein, an F₂ segregating population was constructed using the high resistance material "NuMex Suave Red" and the extremely susceptible material "c89" for bulked segregant analysis and DNA re-sequencing (BSA-seq). Molecular markers were used to achieve fine mapping, followed by expression verification. A major QTL located on chromosome 5 (Chr5, 7.20-11.75 Mb) that is associated with resistance to powdery mildew in pepper was mapped using BSA-seq. A narrow interval of 64.86 kb encompassing five genes was refined using InDel and KSAP molecular markers developed from the QTL region. Among them, the expression of the ubiquitin-conjugating enzyme E2 gene, Capana05g000392, was significantly upregulated in multiple resistant materials. In addition, there was a single nucleotide polymorphism (SNP) of A/G in the 241st position of the CDS sequence of Capana05g000392, which in turn leads to an amino acid polymorphism of M/V between susceptible parent and resistant parent. Overall, these results indicate that the Capana05g000392 gene may serve as a robust potential factor against powdery mildew in pepper. These findings further elucidate the genetic mechanism of resistance to powdery mildew in pepper and facilitate molecular marker-assisted breeding.

While UV-B radiation is beneficial to plant growth, it can also cause adverse effects. The pollen tube, a key component of plant reproduction with a tip growth mechanism, is an excellent cellular model for understanding how environmental stressors such as UV-B radiation affect plant cell growth. This research investigated the effect of UV-B on olive pollen both before and after germination. Pollen grains were hydrated and exposed to UV-B radiation for 1 h. Pollen tube germination was then evaluated 4 and 24 h after exposure. To study the effect of UV-B radiation on developing pollen tubes, pollen was germinated for 4 h prior to 1 h of UV-B exposure. Pollen tube growth was evaluated by assessing the distribution of cell wall components, the distance between callose plugs and nuclei, and the distance between the male germ unit and the pollen tube tip. We also examined the accumulation of callose synthase. The results showed that UV-B radiation significantly inhibited the growth of pollen tubes, thereby preventing successful fertilization. The effect of UV-B exposure on pollen tube growth was mainly due to the alteration of position of callose plugs and the level of callose synthase in the pollen tube, potentially affecting its growth. In addition, UV-B radiation affected the movement and integrity of the male germ unit, a critical element for successful fertilization. This research sheds light on how UV-B radiation affects the growth of pollen tubes and highlights the need for further research into the effects of UV-B radiation on plant cells and plant reproduction.

Ground-level ozone (O₃) is well recognized as a secondary air pollutant with detrimental effects on plant growth and biochemistry. In a field study, Andrographis paniculata (King of Bitter) was exposed to ambient O₃ and elevated O₃ (AO + 20 ppb) at three growth stages [45, 90, and 135 days after treatment, (DAT)] using open-top chambers. Elevated O₃ stress negatively impacted plant growth, increased cell damage, and induced foliar injuries. However, elevated O₃ also boosted antioxidant production such as proline, phenol, and enzymatic antioxidants, as well as certain secondary metabolites such as tannins, phytosterols, saponins, and alkaloids. This may enhance the plant's medicinal properties, including compounds limonene dioxide, phytol, palmitic acid, and androstadiene. While, certain metabolites like Citronellol, Khusenol, and tocopherol displayed an adverse reaction under elevated O₃ exposure. The novel detection of acrodiene, squalene, and neophytadiene under O₃ stress emphasizes their medicinal significance. Notably, an important bioactive compound andrographolide in A. paniculata showed increased synthesis under elevated O₃ at 45 and 90 DAT, suggesting that O₃ exposure could enhance the plant's pharmaceutical value.

I present here a rebuttal to an article in this volume wherein Kingsland and Taiz (2024) cast aspersions about an article I have written concerning Sir Jagadis Chandra Bose (Minorsky PV, in Plant Signal Behav 16:1818030, 2021) a brilliant Bengali scientist who was a pioneer not only in physics (microwaves and semi-conductors), but also in elucidating the electrophysiological responses of plants to environmental stimuli. The charge of racism that I have levelled at Bose's most powerful and well-connected botanical adversary in the 1920s, Daniel T. MacDougal, is irrefutable: MacDougal was a racist, his racism extended to South Asians, and he used racist epithets in referring to Bose. MacDougal offered no cogent arguments against Bose's electrophysiological measurements but attacked Bose with the racist trope that South Asians were "mystics." MacDougal wielded his political and editorial clout to publicize faulty research in opposition to Bose while ignoring a sizable body of contemporaneous evidence in support of Bose's ideas. Unfortunately, given MacDougal's stature as the General Secretary of the American Association for the Advancement of Science (AAAS) and the racist tenor of the time, many Western scientists were too ready to accept uncritically MacDougal's proclamations that Bose was a fraud, an incompetent, and a "Hindoo" mystic. Bose was one of the greatest minds to ever contemplate plant function. It is high time that we, in the West, redress this historical wrong, and acknowledge Bose's enormous and revolutionary contributions to plant physiology.

Forskolin, a diterpenoid found in the roots of Coleus forskohlii, has generated significant interest in the medical field due to its various therapeutic uses. This study aimed to establish an effective system for regenerating C. forskohlii plants, ensuring a year-round supply of plant material and forskolin production. We tested different concentrations of cytokinins, either alone or combined with auxin, to see their impact on shoot multiplication and growth. We found that a medium supplemented with 1.5 mg L^-1 of meta-topolin (mT) resulted in the highest number of shoots (~ 12.66) and leaves (~ 20) within about 5 days. When mT (1 mg L^-1) was combined with a low amount of auxin (0.05 mg L^-1 NAA), we obtained an even greater number of leaves (~ 23). The shoot regeneration capacity was consistent over five subculture passages, showing minimal variation in mean shoot length and number. During acclimatization, around 91% of the plantlets grown in vermiculite + sand survived. The photosynthetic pigment concentration in the plantlets modestly increased in the first 10 days and reached its highest level after 30 days. Genetic fidelity assays using inter simple sequence repeats (ISSRs) confirmed the similarity between the in vitro derived plantlets and the mother plant. Micro-morphological features of in vitro and ex-vitro acclimated plantlets also matched those of the mother plant, further confirming genetic accuracy. Histochemical staining with vanillin confirmed the presence of forskolin in the in vitro roots, indicated by the violet coloration in the cells. Forskolin quantification was also validated by HPLC where in vitro derived roots were documented to undergo an almost ~ 1.8-fold in comparison to that of the mother plant. This established protocol can effectively address resource scarcity for commercial-scale forskolin production and sustainable conservation techniques.

We reply to the response by P Calvo, V Raja, and M Segundo-Ortin to our article titled "Plant 'intelligence' and the misuse of historical sources as evidence." Their response draws on the authority of psychologist Edward C. Tolman in support of their suggestion that the study of plant intelligence requires an interdisciplinary approach, including cognitive science and other disciplines. We argue that there is no justification for using Tolman as an authority in support of the study of plant intelligence. For Tolman, psychology was confined to the study of organisms with brains, and therefore his comment, when taken in context, has no bearing on the subject of plant intelligence. Calvo et al.'s use of this quotation is a further example of the misuse of a historical authority to support their claim that disciplines such as cognitive science can be applied to the study of those plant behaviors that they consider to be "intelligent."

Detarium microcarpum, is a species confined to drier regions of west and central Africa used to treat various diseases including cancer. Phytochemical screening revealed the presence of secondary metabolites (alkaloids) The aim of this work is to study the effect of total aqueous extracts and alkaloid fractions from D. microcarpum leaves, bark and roots on Nicotiana tabacum L. cv. 'Bright Yellow 2' (BY-2) tobacco cell line GFP-TuA3 expressing a N-terminal fusion of GFP. The plant was harvested in two different regions of Mali with a contrasting climate. The effects of the extracts on the microtubules was followed by spinning disc confocal microscopy. We showed that the anti-microtubular effect of the extracts is dose-dependent, depends of the sampling site and the part of the plant used. Total alkaloids extracted of D. microcarpum bark have more effect on microtubules than leaf and root. The bioactivity-guided fractionation should be used to screen out the biologically active compounds of the total alkaloid extracts of the bark of D. microcarpum.

Intelligence is a fundamental property for all life enabling an increased probability of survival and reproduction under wild circumstances. Kingsland and Taiz (2024) think that plants are not intelligent but seem unaware of the extensive literature about intelligence, memory, learning and chromatin topology in plants. Their views are consequently rejected. Their claim of fake quotations is shown to result from faulty reasoning and lack of understanding of practical biology. Their use of social media as scholarly evidence is unacceptable. Darwin's views on intelligence are described, and their pertinence to the adaptive responses of plants is discussed. Justifications for comments I have made concerning McClintock and her "thoughtful" cell, von Sachs writings as indicating purpose (teleonomy) to plant behaviour, Went and Thimann's allusions to plant intelligence and Bose legacy as the father of plant electrophysiology are described. These scientists were usually first in their field of knowledge, and their understanding was consequently deeper. The article finishes with a brief critical analysis of the 36 scientists who were used to condemn plant neurobiology as of no use. It is concluded that participants signed up to a false prospectus because contrary evidence was omitted.

High-affinity K⁺ (HKT) transporters which mediate Na⁺-specific transport or Na⁺-K⁺ co-transport play a key role in plant salt tolerance. In our previous functional study in Xenopus oocytes, we demonstrated that the durum wheat TdHKT1;4-1 acts as a Na⁺-selective transporter. Here, we investigated the function of TdHKT1;4-1 and its contribution in salt stress tolerance in the Arabidopsis athkt1 mutant background. Our results revealed that TdHKT1;4-1 partially complements the salt sensitivity phenotype of the athkt1 transgenic lines. Comparative physiological analyses and oxidative stress status under moderate salt stress (50 mM NaCl) showed that both transgenic lines SH3 and SH5 restored the salt stress tolerance comparable to the level observed in Wt plants. Whereas, under severe salt stress treatment (100 mM NaCl), the athkt1 transgenic lines exhibited an intermediate salt stress tolerance between Wt and athkt1 mutant. Moreover, TdHKT1;4-1 was highly expressed in leaves under moderate and severe salt stress, while in roots, it was largely expressed only under severe salt stress. In addition, antioxidant enzymes such as catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) were significantly expressed in SH3 and SH5 lines compared to athkt1 and Wt under moderate stress. Therefore, TdHKT1;4-1 seems to differ from its Arabidopsis homologous counterpart, as it contributes to salt stress tolerance up to a specific threshold, above which the TdHKT1;4-1 expression may lead to higher root Na⁺ influx, hence increasing its toxicity during salt stress.