Upper ocean temperature and stratification play essential roles in regulating hydrological processes from regional to basin scales and in shaping climate systems. The Eastern Tropical Indian Ocean (ETIO), as a major source of heat and moisture, is critical to regional and large-scale hydroclimatic variability. However, the long-term evolution of its upper water thermal structure remains poorly constrained. Here, we reconstruct sea surface temperature (SST) and thermocline water temperature (TWT) records spanning the past 620 ka using planktonic foraminiferal Mg/Ca ratios from Core 18548 and IODP Site U1482 off the northwest Australian margin. The results reveal that both SST and TWT exhibit pronounced glacial–interglacial cyclicity, primarily paced by orbital-scale forcing, including the 100-ka and precession (23/19-ka) periodicities. Notably, TWT displays a stronger precession signal than SST. These orbital-scale variations reflect the modulation of heat transfer to the ETIO through sea-level-controlled Indonesian Throughflow (ITF) dynamics and precession-driven atmospheric circulation. The amplified TWT response arises from the combined effects of ITF-mediated thermocline advection and insolation-induced vertical mixing, which together regulate upper ocean heat redistribution. A significant shift in the gradient between SST and TWT (ΔT) around 400 ka, coinciding with the MBE, indicates a major reorganization of upper ocean thermal structure, likely driven by glacioeustatic sea-level fluctuations and changes in precession-induced wind systems. Our findings highlight the coupled forcing of 100-ka cycle and precession on upper ocean thermal evolution in the ETIO and offer critical insights into orbital-scale heat redistribution and tropical ocean-climate interactions.
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