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Protein-Based Nanostructures: A Promising Trend in Food Industry 基于蛋白质的纳米结构:食品工业的一个有前途的趋势
Q4 Earth and Planetary Sciences Pub Date : 2023-08-15 DOI: 10.25303/2709rjce1230133
Manjusha ., R. Singh, A. Srivastava
Nanotechnology has shown remarkable progressive innovations in the various fields of biology and biochemistry. Worldwide applications of nanotechnology can be best described in the areas of biomedical applications, drug delivery systems, biosensing and biocatalysis, vaccine designing and development etc. Nanotechnology with its recent developments and advancements brings about a huge impact on the food sector. Proteins due to their biocompatibility and biodegradability, are widely used as an essential part of food-grade nanomaterials. Protein-based nanostructures made from different kinds of proteins provide new options for nanotechnology in the food industry through their innovative range of food applications for processing, packaging, the shelf life of foods, quality control and enhancement of nutritional value. This study reviews the highlights of the recent developments of protein-based nanostructures such as nanocapsules, nanoemulsions, nanocoatings, nanohydrogels and nanosensors in the food industry.
纳米技术在生物学和生物化学的各个领域显示出显著的进步创新。纳米技术在世界范围内的应用可以最好地描述为生物医学应用、药物输送系统、生物传感和生物催化、疫苗设计和开发等领域。纳米技术的发展和进步给食品行业带来了巨大的影响。蛋白质由于其生物相容性和可生物降解性,被广泛用作食品级纳米材料的重要组成部分。由不同种类的蛋白质制成的蛋白质纳米结构为食品工业的纳米技术提供了新的选择,通过它们在食品加工、包装、食品保质期、质量控制和提高营养价值方面的创新应用范围。本文综述了蛋白质基纳米结构在食品工业中的最新进展,如纳米胶囊、纳米乳液、纳米涂层、纳米水凝胶和纳米传感器。
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引用次数: 0
The genomic effects of radiation exposure: induction of cancers and genetic aberrations in mammalian cells 辐射暴露的基因组效应:哺乳动物细胞中癌症和遗传畸变的诱导
Q4 Earth and Planetary Sciences Pub Date : 2023-08-15 DOI: 10.25303/2709rjce1120122
Pankaj Garg
Radiation exposure can be a two-edged sword. On one hand, the deployment of radiotherapy is one of the most acceptable modalities for malignancy treatment, for the reason of its ability to kill cancer cells. Equally, one is scared of the exposure to man-made emissions in the form of nuclear disasters or related to any form of hilarious power generation during the war. It is rational to facilitate the body enduring small intensity of emissions without any marked side effects. However, there are countless detrimental side effects of being exposed to elevated levels of radiation. These harmful side effects form an underlying fear in the general public in understanding the actual mechanism and concrete remediation of radiation exposure. The present study investigates in detail the principles of radiation safety and how different types of radiation emissions affect the human body when exposed at different levels of their intensity. Furthermore, it is also conferred how cellular mutations are originated in different organs and body systems due to different levels of radiation exposure and also the potential of organisms to develop resistance (threshold limit) to counteract harmful effects of radiation.
辐射暴露是一把双刃剑。一方面,放疗的部署是恶性肿瘤治疗中最可接受的方式之一,因为它能够杀死癌细胞。同样,人们害怕暴露在以核灾难的形式出现的人为排放物中,或者与战争期间任何形式的搞笑发电有关。在没有任何明显副作用的情况下,促进人体承受小强度的排放是合理的。然而,暴露在高水平的辐射中会产生无数有害的副作用。这些有害的副作用使公众在了解辐射照射的实际机制和具体补救措施时产生了潜在的恐惧。本研究详细探讨了辐射安全的原理,以及不同类型的辐射在不同强度下对人体的影响。此外,还讨论了细胞突变是如何在不同的器官和身体系统中由于不同水平的辐射暴露而产生的,以及生物体为抵消辐射的有害影响而产生耐药性(阈值限制)的潜力。
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引用次数: 0
Phytoligands analysis of aqueous seed extract of Momordica charantia Linn. via GC-MS for its antidiabetic activity 苦瓜种子水提物的植物配体分析。通过GC-MS检测其抗糖尿病活性
Q4 Earth and Planetary Sciences Pub Date : 2023-08-15 DOI: 10.25303/2709rjce075082
Manju Bala, Anju Dhiman, Munish Garg, Harish Dureja
In India, Momordica charantia Linn. is utilized in juices, health supplements and colouring agents. The aim of the current study is to analyze phytoligands by utilizing gas chromatography mass spectroscopy of aqueous seed extract of Momordica charantia Linn (MCA). The MCA was prepared by macerating the powdered seeds for 72 hrs followed by filtration and lyophilization followed by an in vitro α-glucosidase inhibitory activity. MCA was the potent inhibitor of α-glucosidase with IC50 value 73.75± 0.05 μg/ml. The analysis through GC-MS investigated 60 phytoconstituents which were validated using Schrodinger suite version 2020-3 against α-glucosidase (PDB ID- 5NN5). As per docking results, the existing phytochemicals azetidin-2-one 3,3-dimethyl-4-(1-aminoethyl)-, alpha-l-rhamnopyranose, piperidine, 3-methyl-, 1,4-Dioxane-2,5-dione,3,6-dimethyl and citronellol epoxide (R or S) from MCA, got the highest d-score of -7.230, -5.918, -5.643, -5.312 and -5.185 kcal/mol and could be promising antidiabetic drug candidates.
在印度,Momordica charantia Linn。用于果汁、保健品和着色剂。采用气相色谱质谱法对苦瓜种子水提物中的植物配体进行分析。将种子粉末浸泡72小时,过滤、冻干,体外抑制α-葡萄糖苷酶活性,制备MCA。MCA是α-葡萄糖苷酶的有效抑制剂,IC50值为73.75±0.05 μg/ml。采用气相色谱-质谱法对60种植物成分进行了分析,并利用Schrodinger suite version 2020-3对α-葡萄糖苷酶(PDB ID- 5NN5)进行了验证。对接结果显示,现有植物化学物质azetitin -2- 1,3 -二甲基-4-(1-氨基乙基)-、α -l-鼠李糖、胡椒碱、3-甲基-、1,4-二氧嘧啶-2,5-二酮、3,6-二甲基和环氧香茅醇(R或S)的d值最高,分别为-7.230、-5.918、-5.643、-5.312和-5.185 kcal/mol,有望成为抗糖尿病的候选药物。
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引用次数: 0
A Sensitive and High Throughput LC-MS/MS Method for the Determination of Resveratrol in Peanut Matrix 高效液相色谱-质谱联用法测定花生基质中白藜芦醇的含量
Q4 Earth and Planetary Sciences Pub Date : 2023-08-15 DOI: 10.25303/2709rjce046052
Vidya Sagar Venkata Pasupuleti, Prashant Bagade, Naga Suresh Reddy Venkata Kachireddy
A precise and very efficient tandem mass spectrometry method (LC-MS/MS) was developed to quantify the resveratrol in the peanut matrix. Two mobile phases made up of water and acetonitrile in a ratio of 98:2 (%v/v) with 0.1% formic acid and acetonitrile and water in a percentage of 98:2 %(v/v) with 0.1% formic acid were used in the LC-MS/MS analysis of resveratrol on a BEH C-18, 130A° (50mm 2.1mm, 1.7μm) analytical column with 0.3 mL/min of flow rate. A triple quadrupole mass spectrometer and a positive electrospray ionization system were used to quantify resveratrol in multiple reaction monitoring (MRM) modes. The technique was evaluated and was found capable of quantifying resveratrol in peanut samples starting at 10 ng/g in compliance with SANTE guidelines.
建立了一种精确高效的串联质谱(LC-MS/MS)定量花生基质中白藜芦醇的方法。采用hplc -MS/MS分析白藜芦醇,色谱柱为BEH C-18, 130°(50mm 2.1mm, 1.7μm),流速为0.3 mL/min,流动相为水与乙腈的比例为98:2 (%v/v)与0.1%甲酸的比例为98:2 (v/v),流动相为乙腈与水的比例为98:2% (v/v)与0.1%甲酸。采用三重四极杆质谱仪和正电喷雾电离系统,在多反应监测(MRM)模式下定量白藜芦醇。对该技术进行了评估,发现能够定量花生样品中的白藜芦醇,从10纳克/克开始,符合SANTE指南。
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引用次数: 0
Practical Design of Hybrid Photobioreactor System for Economic Feasibility and Environmental Sustainability 经济可行性和环境可持续性混合光生物反应器系统的实用设计
Q4 Earth and Planetary Sciences Pub Date : 2023-08-15 DOI: 10.25303/2709rjce017027
Bill Sun
Modern photobioreactors for microalgae-based biofuel production face various budgetary challenges in comparison to its competitor design, the traditional open pond system. Herein, a novel design of a double-chamber hybrid photobioreactor is proposed, featuring low-cost components including a 3-W centrifugal pump and recyclable plastic modules for its solar flank, as well as derivatives from tubular and membrane photobioreactors. The hybrid photobioreactor exhibits reasonable hydrodynamic performance with a circulation time of 120 seconds and a mixing time of 38 seconds. A side-by-side runthrough of Chlorella vulgaris cultivation with the novel hybrid photobioreactor and a standard open pond system was done to compare growth rates and system performance under equal environmental conditions. Resulting measurements show that the hybrid photobioreactor produced a 34.87% comparatively greater amount of C. vulgaris by the tenth day of cultivation, demonstrating a significantly higher growth rate (P < 0.05). In contrast, wet-sludge lipid extraction processes show that the C. vulgaris culture grown in the open pond system held a substantially larger lipid accumulation than that of the hybrid photobioreactor, although the difference was not statistically significant (P > 0.05). The pilot-scale photobioreactor not only costed as little as the open pond system but also incurred 88.6% less expenses compared to a similarly designed bioreactor made of clear PVC material. Consequently, this project demonstrates the candidacy of the proposed low-cost hybrid photobioreactor design for microalgal biofuel production.
用于微藻生物燃料生产的现代光生物反应器与其竞争对手传统开放池塘系统相比,面临着各种预算挑战。本文提出了一种新型的双室混合型光生物反应器,其特点是采用低成本组件,包括3-W离心泵和太阳能侧翼的可回收塑料模块,以及管状和膜状光生物反应器的衍生物。混合式光生物反应器具有合理的水动力性能,循环时间为120秒,混合时间为38秒。采用新型混合光生物反应器和标准开放池塘系统对普通小球藻进行了并排试验,比较了在相同环境条件下的生长速度和系统性能。结果表明,到培养第10天,混合型光生物反应器产生的C. vulgaris相对增加了34.87%,显示出显著提高的生长速度(P <0.05)。相比之下,湿污泥脂质提取过程表明,在开放池塘系统中生长的C. vulgaris培养物的脂质积累量明显大于混合光生物反应器,尽管差异没有统计学意义(P >0.05)。中试规模的光生物反应器不仅成本与开放池塘系统一样低,而且与由透明PVC材料制成的类似设计的生物反应器相比,费用减少了88.6%。因此,该项目证明了所提出的用于微藻生物燃料生产的低成本混合光生物反应器的候选性。
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引用次数: 0
Investigation of the Synthesis, Characterization and Properties of Nano Cu2Zr3O7 – HDPE Composite Sheets 纳米Cu2Zr3O7 - HDPE复合片材的合成、表征及性能研究
Q4 Earth and Planetary Sciences Pub Date : 2023-08-15 DOI: 10.25303/2709rjce920101
Koteswara Rao Jammula, Sagar, J.S., Madhu G.M., Pradipkumar Dixit
This study reports on the synthesis of Cu2Zr3O7 nanoparticles using the solution combustion method followed by the fabrication and characterization of micro-composite sheets of Cu2Zr3O7 - HDPE. The characterization was performed using SEM, XRD and EDX analysis which demonstrated the effects of nano-sized Cu2Zr3O7 particles on the crystallinity and surface morphology of the fillers and elemental analysis using the EDX method. The dielectric behavior of the composite was investigated using an Impedance Analyzer. The composite's tensile strength was increased as evidenced by 34% increase in Young's modulus compared to blank HDPE for the sample with 1.5 wt% nanofiller loading. The results indicated that the dielectric constant increased as the frequency decreased, with higher values observed at 10 MHz. However, this trend was less pronounced at reduced filler loadings. The loss tangent initially dropped abruptly and then gradually as the frequency increased between 4 Hz and 1 MHz. The addition of Cu2Zr3O7 nanoparticles resulted in increased conductivity at higher frequencies compared to pure HDPE, with no significant frequency dependency observed at lower frequencies.
本文报道了采用溶液燃烧法制备Cu2Zr3O7纳米颗粒,并对Cu2Zr3O7 - HDPE微复合片材进行了制备和表征。采用SEM、XRD、EDX等方法对Cu2Zr3O7进行了表征,验证了纳米Cu2Zr3O7对填料结晶度和表面形貌的影响,并用EDX方法进行了元素分析。用阻抗分析仪研究了复合材料的介电性能。复合材料的抗拉强度增加了34%,与空白HDPE样品的杨氏模量相比,纳米填料加载量为1.5%。结果表明,介电常数随频率的降低而增大,在10 MHz时介电常数较大。然而,这种趋势在减少填料负载时不太明显。在4hz ~ 1mhz范围内,随着频率的增加,损耗正切值开始突然下降,然后逐渐下降。与纯HDPE相比,添加Cu2Zr3O7纳米颗粒在高频下的电导率提高,而在低频下没有明显的频率依赖性。
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引用次数: 0
Investigation of Antibacterial Activity of Ag-CuO and Ag-ZnO Nanocomposites synthesized by Chemical Precipitation Method 化学沉淀法合成Ag-CuO和Ag-ZnO纳米复合材料的抗菌活性研究
Q4 Earth and Planetary Sciences Pub Date : 2023-08-15 DOI: 10.25303/2709rjce06068
Priya P. Vengatesh, J. Jeyasundari, Athithan A.S. Sakthi, A. Naveena
In this present study, the synthesis of Ag-CuO and Ag-ZnO nanocomposites has been conducted. The individual Ag-CuO and Ag-ZnO nanocomposites were synthesized by using chemical precipitation method. The resulting particles were characterized by using UV-Visible, FTIR, XRD and SEM. The optical properties and band gap measurements were explored by UV-Visible spectroscopy. FTIR spectrum of the prepared nanocomposite revealed the presence of vibrational modes which were related to the Cu-O, Zn-O. The XRD analysis confirmed the structural purity of synthesized nanocomposites and the estimated crystallite size is 31.93 nm and 26.62 nm for Ag-CuO and Ag-ZnO nanocomposites respectively. The morphological features were explored by SEM analysis with deposition of Ag nanoparticles on the surface of metal oxide nanoparticles. The antibacterial activities of synthesized nanocomposites were tested on bacteria strains such as Escherichia coli (Gram -ve) and Staphylococcus aureus (Gram +ve) respectively through well diffusion method. The synthesized nanocomposite shows the higher efficacy against E.coli with an average diameter size of 17 mm zone of inhibition. This result suggests that Ag-CuO and Ag-ZnO nanocomposites can be used effectively against microbial growth. Therefore, the synthesized nanocomposite may be promising for the antibacterial agent in pharmaceutical applications.
本文研究了Ag-CuO和Ag-ZnO纳米复合材料的合成。采用化学沉淀法分别合成了Ag-CuO和Ag-ZnO纳米复合材料。通过紫外可见光谱、红外光谱、x射线衍射和扫描电镜等手段对所得颗粒进行了表征。利用紫外-可见光谱法对其光学性质和带隙测量进行了研究。制备的纳米复合材料的FTIR光谱显示存在与Cu-O, Zn-O相关的振动模式。XRD分析证实了合成的纳米复合材料的结构纯度,估计Ag-CuO和Ag-ZnO纳米复合材料的晶粒尺寸分别为31.93 nm和26.62 nm。通过扫描电镜分析银纳米颗粒沉积在金属氧化物纳米颗粒表面的形貌特征。采用孔扩散法对合成的纳米复合材料分别对大肠杆菌(Gram -ve)和金黄色葡萄球菌(Gram +ve)的抑菌活性进行了测试。合成的纳米复合材料对大肠杆菌具有较高的抑制作用,平均直径为17 mm。这表明Ag-CuO和Ag-ZnO纳米复合材料可以有效地抑制微生物的生长。因此,所合成的纳米复合材料在抗菌药物领域具有广阔的应用前景。
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引用次数: 0
A novel approach of 19F NMR based sensor 一种基于19F核磁共振传感器的新方法
Q4 Earth and Planetary Sciences Pub Date : 2023-08-15 DOI: 10.25303/2709rjce1340144
Amit Kumar Khandelwal, Poonam Hariyani, Barkha Shrivastava
Reliable and precise methods are to be applied ranging from biological field to diagnosis and quality control to identify target analytes in samples. Nuclear magnetic resonance (NMR) is a strongly recommended process that is suitable for collecting data multidimensionally at molecular and atomic levels. Information obtained from the NMR technique provides molecular structures, molecular interactions, dynamic and diffusional properties of molecules. Overlapping of NMR signals of each component makes it difficult to interpret data and low sensitivity makes it unfitting for many samples with a low concentration of analytes. These limitations can be rectified by adding heteronuclear specially designed sensors, inventive transduction mechanism and strong pulse sequences. 19F NMR tool is extensively accepted method to detect the interactions among a variety of biological substrates with 19F-isotopic species. This review includes the detection of biomolecules, mainly enzymes and enzymatic reactions based on sensor applicability.
从生物领域到诊断和质量控制,都需要可靠和精确的方法来识别样品中的目标分析物。核磁共振(NMR)是一种强烈推荐的方法,适合在分子和原子水平上多维地收集数据。从核磁共振技术获得的信息提供了分子结构、分子相互作用、分子的动态和扩散特性。各组分核磁共振信号的重叠使数据解释困难,灵敏度低使其不适合于分析物浓度低的许多样品。这些限制可以通过添加特殊设计的异核传感器,发明的转导机制和强脉冲序列来纠正。19F核磁共振工具是一种被广泛接受的检测各种生物底物与19F同位素之间相互作用的方法。本文综述了基于传感器适用性的生物分子,主要是酶和酶反应的检测。
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引用次数: 0
Effect of Guar Gum on Tensile Strength and Moisture Resistance Properties of Sodium Silicate as Wood Adhesive 瓜尔胶对硅酸钠木材胶粘剂抗拉强度和抗湿性能的影响
Q4 Earth and Planetary Sciences Pub Date : 2023-08-15 DOI: 10.25303/2709rjce083091
Manju Choudhary, Bhupendra Pal, Meenakshi .
Sodium silicate has excellent binding qualities and has potential uses in a variety of areas due to its non-toxic, odor-free and fire proof properties. It is very cheap and volatile organic compound free from other available harmful organic adhesive materials. In spite of a lot of positive points, low water resistance has limited its usage as an adhesive in the wood industry on a wide scale. To enhance the bonding properties of sodium silicate for teak wood, three sets of sodium silicate adhesive (simple sodium silicate, modified sodium silicate with silica gel G and guar gum with modified sodium silicate) were prepared. The results showed that modified sodium silicate with 6% guar gum works best for tensile strength as well as best for water resistance. Results of FT-IR data and SEM showed that guar gum was successfully introduced in silicate structure, having good thermal stability in the range of 270C-8000C supported by TGA results.
硅酸钠具有优良的粘结性能,由于其无毒、无臭和防火的特性,在各种领域都有潜在的用途。它是一种非常便宜的挥发性有机化合物,不含其他现有的有害有机粘合材料。尽管有很多优点,但低耐水性限制了它在木材工业中作为粘合剂的广泛使用。为提高硅酸钠与柚木的粘合性能,制备了简单硅酸钠、改性硅酸钠和改性硅酸钠瓜尔胶三种硅酸钠胶粘剂。结果表明,6%瓜尔胶改性水玻璃的抗拉强度和耐水性最好。红外光谱(FT-IR)和扫描电镜(SEM)结果表明,瓜尔胶被成功地引入到硅酸盐结构中,热稳定性良好,温度范围在270C-8000C之间。
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引用次数: 0
Spatial and Temporal variations of Organic Chemical compounds in Ambient Air from Ahmedabad City, Gujarat, India 印度古吉拉特邦艾哈迈达巴德市环境空气中有机化合物的时空变化
Q4 Earth and Planetary Sciences Pub Date : 2023-08-15 DOI: 10.25303/2709rjce028038
Himalay Bhakhar, Rita N. Kumar, Shwetal Shah, Nirmal J.I. Kumar
Organic contaminants linked to atmospheric particulate matter have been a major source of worry across the world since the last decade. The ambient air samples were collected from three sites in Ahmedabad city for spatial and temporal variation using a High-Volume Air Sampler (HVAS) to examine the morphology and elemental composition of fine and ultrafine particles by using a Scanning electron microscope (SEM) and an energy dispersive X-ray system (EDAX) from PM2.5. The Si, C, O, Cl, Na, Al, K, Ca and Mg were found to be quite abundant in the EDAX spectra. Transition elements such as Ti, Zn, Cu, S and Fe were registered, indicating the influence of industry in liberating the abundance of fine and ultrafine particles. In addition, the organic chemical compounds were screened by using GC–MS. A total of 230 organic chemicals were detected, including 187 aliphatic and 43 aromatic chemical compounds at three different locations. Results show that aliphatic and aromatic hydrocarbon compounds were found maximum in the urban area followed by industrial and rural areas.
自过去十年以来,与大气颗粒物有关的有机污染物一直是全球担忧的主要来源。采用高容量空气采样器(High-Volume air Sampler, HVAS)采集了艾哈迈达巴德市3个站点的环境空气样本,分析了PM2.5的时空变化特征,并利用扫描电子显微镜(SEM)和能量色散x射线系统(EDAX)研究了PM2.5细颗粒和超细颗粒的形态和元素组成。在EDAX光谱中发现了相当丰富的Si、C、O、Cl、Na、Al、K、Ca和Mg。过渡元素如Ti、Zn、Cu、S、Fe等被记录下来,表明工业对释放细颗粒和超细颗粒丰度的影响。此外,采用气相色谱-质谱法对有机化合物进行了筛选。在三个不同的地点共检测到230种有机化学品,其中包括187种脂肪族化合物和43种芳香化合物。结果表明,城市地区以脂肪族和芳烃化合物含量最多,其次是工业地区和农村地区。
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引用次数: 0
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Research Journal of Chemistry and Environment
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