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Characterization and optimization of bacterium isolated from soil samples for the production of siderophores 从土壤样品中分离的产铁载体细菌的特性和优化
Pub Date : 2017-12-01 DOI: 10.1016/j.reffit.2017.04.004
Venkat Kumar S., Soumya Menon, Happy Agarwal, Divya Gopalakrishnan

Siderophores are small molecules that can easily bind to ferric iron. As a chelating agent, they transport iron molecules inside the bacterial cell for various biochemical reactions. Due to its various applications in medicinal, industrial and environmental related aspects, this paper deals with characterization and optimization of few siderophores producing bacteria from the soil samples, collected from Chikkamagaluru district, Karnataka. The siderophores production was assayed qualitatively and quantitatively through Chrome Azurol S and the results showed positive for the strains VITVK5 and VITVK6 that grown in succinate medium. Further characterization and optimization results revealed that both the bacterium has the ability to yield siderophores (∼60–80% units) in the optimum condition of pH 8, at 37°C with glucose and sucrose as a carbon source and NaNO3 as a nitrogen source. Thus, the study concludes that strains VITVK5 and VITVK6 can be promising candidates for the siderophores production which can play major applications in medicinal and industrial aspects.

铁载体是很容易与铁结合的小分子。作为螯合剂,它们在细菌细胞内运输铁分子进行各种生化反应。由于其在医药、工业和环境相关方面的各种应用,本文讨论了从卡纳塔克邦Chikkamagaluru地区收集的土壤样品中几种产生铁载体的细菌的表征和优化。通过Chrome Azurol S对其产铁载体进行了定性和定量检测,结果表明菌株VITVK5和VITVK6在琥珀酸培养基中均呈阳性。进一步的表征和优化结果表明,这两种细菌在pH为8、温度为37°C、葡萄糖和蔗糖为碳源、NaNO3为氮源的最佳条件下都能产生铁载体(约60-80%单位)。因此,该菌株VITVK5和VITVK6是生产铁载体的候选菌株,在医药和工业方面具有重要的应用前景。
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引用次数: 56
Performance investigation of vapour recompressed batch distillation for separating ternary wide boiling constituents 蒸汽再压缩间歇精馏分离三元宽沸组分的性能研究
Pub Date : 2017-12-01 DOI: 10.1016/j.reffit.2017.04.007
Rohith R Nair, Uday Bhaskar Babu G, Amol Raykar

The vapour recompression scheme (VRC) has been very effective in continuous distillation for energy intensification. The applicability of this scheme for the separation of multicomponent wide boiling constituents in batch distillation is a major challenge, because of the unsteady nature of the batch. In this study, the vapour recompression scheme has been implemented for the separation of multicomponent wide boiling constituents in the batch distillation. For the optimal usage of energy from compressed vapours manipulation of top tray vapour or external energy is done. A comparative study of the vapour recompressed batch distillation having a variable speed single compressor (SVRBD) and double stage compressor (DVRBD) with conventional batch distillation in terms of energy savings and total annualized cost is done. The VRC schemes achieve an energy savings of 50% and 10.03% total annualized cost (TAC) for SVRBD and DVRBD achieve 52% energy savings and 12.21% TAC with a payback period of 10 years.

蒸汽再压缩方案(VRC)在连续精馏中用于能量强化是非常有效的。由于间歇精馏的不稳定特性,该方案在间歇精馏中多组分宽沸组分分离中的适用性是一个重大挑战。本研究采用蒸汽再压缩方法对间歇精馏中多组分宽沸组分进行分离。为了最大限度地利用来自压缩蒸汽的能量,对顶部托盘蒸汽或外部能量进行操纵。对采用变速单压缩机(SVRBD)和双级压缩机(DVRBD)的蒸汽再压缩间歇精馏与常规间歇精馏的节能效果和年化总成本进行了比较研究。VRC方案实现了50%的能源节约和10.03%的总年化成本(TAC), SVRBD和DVRBD实现了52%的能源节约和12.21%的TAC,投资回收期为10年。
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引用次数: 6
A review on biogenic synthesis of gold nanoparticles, characterization, and its applications 纳米金的生物合成、表征及其应用研究进展
Pub Date : 2017-12-01 DOI: 10.1016/j.reffit.2017.08.002
Soumya Menon, Rajeshkumar S., Venkat Kumar S.

The nano-sized particles make an imprint on us in our daily lives and it has great importance in the numerous fields of biotechnology like the food industry, medical and industrial field. Gold nanoparticles are one of the widely used particles as it has many therapeutic applications, such as drug delivery system for many diseases like cancer, cardiovascular diseases, diabetes mellitus etc. biosensors, and environmental applications of dye degradation, bioremediation of toxic chemicals present in the environment (soil and atmosphere). Gold nanoparticles synthesis by the green route has become the latest development, because of the bioavailability of sources like plants or microorganisms, and it also reduces the utilization of toxic chemicals. This review explains the various microorganisms like bacteria, algae, fungi, actinomycetes and yeast involved in the synthesis of these nanoparticles also elucidate the size, shape and functional groups involved in the synthesis of nanoparticles and its applications.

纳米粒子在我们的日常生活中留下了深刻的印记,在食品工业、医疗和工业等众多生物技术领域都有着重要的意义。金纳米粒子是广泛应用的粒子之一,因为它具有许多治疗应用,如癌症、心血管疾病、糖尿病等许多疾病的药物递送系统,生物传感器,染料降解的环境应用,存在于环境(土壤和大气)中的有毒化学物质的生物修复。利用绿色路线合成纳米金已成为最新的发展趋势,因为它不仅可以利用植物或微生物等来源的生物利用度,而且还可以减少有毒化学物质的利用。本文介绍了参与纳米颗粒合成的细菌、藻类、真菌、放线菌和酵母等微生物,并对纳米颗粒合成中涉及的大小、形状和官能团及其应用进行了阐述。
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引用次数: 227
Virtual lab based real-time data acquisition, measurement and monitoring platform for solar photovoltaic module 基于虚拟实验室的太阳能光伏组件实时数据采集、测量与监测平台
Pub Date : 2017-12-01 DOI: 10.1016/j.reffit.2017.04.006
Amit Kumar Rohit, Amit Tomar, Anurag Kumar, Saroj Rangnekar

The work presents real-time data acquisition and monitoring of solar photovoltaic modules using LabVIEW. A graphical program has been developed to obtain efficiency and fill factor of solar PV module. A front panel is designed, displaying all the acquired data such as; voltage, current, solar radiation, ambient temperature, humidity, Current vs. Voltage and Power vs. Voltage graphs which make it very useful to understand the performance behavior of the solar photovoltaic module in real time. Data acquisition and monitoring for solar panels of different ratings are carried out. This tool is an effective platform for experimental study in the laboratory of different solar photovoltaic modules with access to real-time data.

本文介绍了利用LabVIEW对太阳能光伏组件进行实时数据采集和监测。开发了太阳能光伏组件效率和填充系数的图形化计算程序。设计了一个前面板,显示所有采集到的数据,如;电压,电流,太阳辐射,环境温度,湿度,电流与电压和功率与电压的图表,这使得实时了解太阳能光伏组件的性能行为非常有用。对不同额定值的太阳能电池板进行了数据采集和监测。该工具是一个有效的实验平台,可以在实验室中对不同的太阳能光伏组件进行实验研究,并获得实时数据。
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引用次数: 22
Mathematical modeling of drying behavior of cashew in a solar biomass hybrid dryer 腰果在太阳能生物质混合干燥器中干燥行为的数学建模
Pub Date : 2017-12-01 DOI: 10.1016/j.reffit.2016.12.002
S. Dhanushkodi , Vincent H. Wilson , K. Sudhakar

The main objective of this study is to analyze the drying behavior of cashew nut experimentally in a solar biomass hybrid dryer using mathematical models. Suitability of fifteen different mathematical drying models available in the literature is used to describe the drying characteristics of cashew. Experimental data of moisture ratio, temperature and relative humidity obtained from different dryer conditions were fitted to the various empirical drying models. The performance of the drying model was compared based on their correlation co-efficient (R2), Root Mean Square Error (RMSE) and Reduced Chi-Square (χ2) between the observed moisture ratios. The two terms and Midilli models showed the best fit under solar drying. Page model was found to be the best model for describing the thin layer drying behavior of cashew for biomass drying and hybrid drying.

本研究的主要目的是利用数学模型对腰果在太阳能生物质混合干燥机中的干燥行为进行实验分析。采用文献中已有的15种不同干燥数学模型的适用性来描述腰果的干燥特性。将不同干燥条件下的湿比、温度和相对湿度的实验数据拟合到不同的经验干燥模型中。根据观测到的水分比之间的相关系数(R2)、均方根误差(RMSE)和减少的卡方误差(χ2)对干燥模型的性能进行比较。两项模型和Midilli模型在日光干燥条件下拟合最佳。在生物质干燥和混合干燥中,Page模型是描述腰果薄层干燥行为的最佳模型。
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引用次数: 64
Optimization studies on extraction of phytocomponents from betel leaves 槟榔叶中植物成分提取工艺的优化研究
Pub Date : 2017-12-01 DOI: 10.1016/j.reffit.2017.02.007
Muruganandam L, Anantha Krishna, Jashwanth Reddy, G.S. Nirmala

The present study aims at finding out the optimum parameters for the extraction of components from Betel leaves possessing medicinal applications using ethanol solvent by Soxhlet apparatus. The optimum conditions for the extract were calculated based on the extract yield by varying four parameters: material quantity (A: 2–4 g), solvent quantity (B: 250–300 ml), mantle temperature (C: 65–75 °C) and extraction time (D: 1–3 hours) and optimized using a four factor three level Box–Behnken response surface design (BBD) coupled with desirability function methodology. Results showed that temperature and extraction time had significant effect on yield of extract. Optimum conditions for highest yield of extract (10.94%) are as follows: material quantity (2 g), solvent quantity (281.4 ml), temperature (72 °C) and time (3 hours). The extract at the maximum yield condition was analyzed for phytocomponents by FTIR and GC–MS. The results indicated the presence of Hydroxy chavicol (69.46%), 4-Chromanol (24%) and Eugenol (4.86%), which possess wide application including as antioxidant, anti-inflammatory, anti-platelet and antithrombotic, antibacterial and antifungal agents.

本研究旨在探讨用索氏仪提取药用槟榔叶中各成分的最佳工艺条件。通过物料用量(A: 2 ~ 4 g)、溶剂用量(B: 250 ~ 300 ml)、包套温度(C: 65 ~ 75℃)和提取时间(D: 1 ~ 3小时)4个参数,计算出提取的最佳条件,并采用四因素三水平Box-Behnken响应面设计(BBD)结合期望函数法进行优化。结果表明,提取温度和提取时间对提取率有显著影响。提取得率最高(10.94%)的最佳条件为:料量2 g,溶剂量281.4 ml,温度72℃,时间3 h。采用红外光谱(FTIR)和气相色谱-质谱(GC-MS)分析了最大产量条件下提取液的植物成分。结果表明,丁香酚中含有羟基丁香酚(69.46%)、4-丁香酚(24%)和丁香酚(4.86%),具有抗氧化、抗炎、抗血小板、抗血栓、抗菌、抗真菌等广泛应用。
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引用次数: 45
A new fuzzy framework for the optimal placement of phasor measurement units under normal and abnormal conditions 提出了一种新的模糊框架,用于正常和异常情况下相量测量单元的优化配置
Pub Date : 2017-12-01 DOI: 10.1016/j.reffit.2017.08.005
Ragab A. El-Sehiemy , Shady H.E. Abdel Aleem , Almoataz Y. Abdelaziz , Murat E. Balci

This paper presents a new procedure for finding the optimal placement of the phasor measurement units (PMUs) in modern power grids to achieve full network observability under normal operating conditions, and also abnormal operating conditions such as a single line or PMU outage, while considering the availability of PMU measuring channels. The proposed modeling framework is implemented using the fuzzy binary linear programming (FBLP) technique. Linear fuzzy models are proposed for the objective function and constraints alike. The proposed procedure is applied to five benchmark systems such as the IEEE 14-bus, 30-bus, 39-bus, 57-bus, and 118-bus. The numerical results demonstrate that the proposed framework is capable of finding a fine-tuned optimal solution with a simple model and acceptable solution characteristics compared with early works in the literature. Besides, four evaluation indices are introduced to assure the various criteria under study such as the observability depth, measurement redundancy, and robustness of the method under contingencies. The results show that full network observability can be met under normal conditions using 20% PMUs penetration; however, under contingencies, approximately 50% PMUs penetration is required. The novelty of the proposed procedure has proven the capability of the proposed linear fuzzy models to find better optimal number of PMUs with lower number of channels compared to other algorithms under various operating conditions. Hence, the proposed work represents a potential tool to monitor power systems, and it will help the operators in a smart grid environment.

本文提出了一种新的方法来寻找现代电网中相量测量单元(PMU)的最佳位置,以实现在正常运行条件下以及在单线或PMU停电等异常运行条件下的完全网络可观测性,同时考虑PMU测量通道的可用性。该建模框架采用模糊二元线性规划(FBLP)技术实现。对目标函数和约束条件都提出了线性模糊模型。将该方法应用于IEEE 14总线、30总线、39总线、57总线和118总线等5个基准系统。数值结果表明,与已有文献相比,所提出的框架能够以简单的模型和可接受的解特性找到一个微调的最优解。此外,还引入了4个评价指标来保证所研究方法的可观测性深度、测量冗余度和偶然性条件下的鲁棒性。结果表明,在正常情况下,pmu渗透率为20%时,可以满足完全的网络可观测性;然而,在意外情况下,大约50%的pmu渗透率是必需的。该程序的新颖性证明了与其他算法相比,所提出的线性模糊模型能够在各种运行条件下以更少的通道数找到更好的最优pmu数量。因此,所提出的工作代表了一种潜在的监测电力系统的工具,它将帮助运营商在智能电网环境中。
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引用次数: 11
An experimental analysis of quenching of continuously heated vertical rod with aqueous Al2O3 nanofluid Al2O3纳米流体连续加热竖杆淬火的实验分析
Pub Date : 2017-12-01 DOI: 10.1016/j.reffit.2017.02.006
Nirupama Patra , Vivek Gupta , Ravi Singh , R.S. Singh , Pradyumna Ghosh , Arun Nayak

Quenching characteristics of metallic vertical rod with an internal heater has been investigated in pure water and water-based nanofluid with alumina nanoparticles of 0.001% by volume. Initial temperature of the testing rod (12 mm diameter and 1000 mm length) ranges from 200 °C to 250 °C. The internal heater may be used to simulate the decay heat generated by the nuclear fuel rod after the power plant shutdown. Tests have been performed with varied range of experimental conditions of decay heat, constant water and nanofluid flow rates. The cooling curves follow a general trend of a rapid temperature drop up to almost 100 °C of the rod surface temperature irrespective of the operating parameters and the location of the thermocouple. The obtained results during quenching process indicated that HTC (heat transfer coefficient) for nanofluids are more than de-ionized water. It was also observed that in identical circumstances, the quenching time of the specimen in Al2O3 nanofluid considerably decreased as compared to water. The results exhibit that nanofluids can enhance the reflood heat transfer performance in terms of quenching rate for a long vertical rod causing liquid-droplets-induced depositions of nanoparticles resulting in making a pre-coating effect characterized by higher wettability.

研究了带内加热器的金属竖杆在含体积为0.001%氧化铝纳米颗粒的纯水和水基纳米流体中的淬火特性。测试棒(直径12mm,长度1000mm)初始温度范围为200℃~ 250℃。内部加热器可用于模拟核电站停堆后核燃料棒产生的衰变热。在衰减热、恒定水和纳米流体流速等不同实验条件下进行了试验。无论操作参数和热电偶的位置如何,冷却曲线都遵循快速温度下降的总体趋势,最高可达棒表面温度的近100°C。在淬火过程中得到的结果表明,纳米流体的传热系数HTC大于去离子水。实验还发现,在相同条件下,试样在Al2O3纳米流体中的淬火时间比在水中明显缩短。结果表明,纳米流体可以通过淬灭速率提高长垂直杆的回流换热性能,导致液滴诱导的纳米颗粒沉积,从而形成具有更高润湿性的预涂层效应。
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引用次数: 23
Green synthesis of silver nanoparticles using Lippia nodiflora aerial extract and evaluation of their antioxidant, antibacterial and cytotoxic effects 绿色合成纳米银及其抗氧化、抗菌和细胞毒作用的评价
Pub Date : 2017-12-01 DOI: 10.1016/j.reffit.2017.07.002
Arumugam Sudha , Jeyaraman Jeyakanthan , Pappu Srinivasan

Silver nanoparticles biosynthesis has received increasing attention in the field of nanotechnology due to their antimicrobial and biomedical applications. Green synthesis of metal nanoparticles is anticipated as a cost effective and eco-friendly alternative in the current research scenario. With this aim, the aqueous extracts made from the aerial parts of Lippia nodiflora were used as the reducing agents to synthesize silver nanoparticles (AgNPs) and their antioxidant, antibacterial and cytotoxic properties have also been evaluated. The biosynthesized AgNPs were characterized by UV–Visible Spectroscopy, Fourier-Transform Infrared Spectroscopy (FTIR) and X-Ray Diffraction (XRD) analysis. The AgNPs were found to be stable at −25.2 mV through zeta potential study. The morphology and size of synthesized silver nanoparticles were confirmed by Scanning Electron Microscope with energy dispersive spectra (SEM-EDX) and Transmission Electron Microscopy (TEM) analysis with size range from 30 to 60 nm. Biosynthesized AgNPs exhibited strong antioxidant activity as well as showed potent antibacterial activity against human pathogenic bacteria. The cytotoxicity study of AgNPs was also revealed against MCF-7 breast cancer cell lines in a dose-dependent manner. The recognized bioactivity confirmed by the synthesized AgNPs directs towards the clinical use as an antioxidant, antibacterial and cytotoxic agent.

银纳米粒子的生物合成由于其抗菌和生物医学方面的应用越来越受到纳米技术领域的关注。在目前的研究中,绿色合成金属纳米粒子有望成为一种经济有效且环保的替代方案。为此,以野野Lippia nodiflora地上部分的水提物为还原剂合成银纳米粒子(AgNPs),并对其抗氧化、抗菌和细胞毒性进行了评价。采用紫外可见光谱、傅里叶红外光谱(FTIR)和x射线衍射(XRD)对合成的AgNPs进行了表征。通过zeta电位研究发现AgNPs在−25.2 mV下稳定。通过扫描电子显微镜(SEM-EDX)和透射电子显微镜(TEM)对合成的银纳米粒子的形貌和尺寸进行了验证,粒径范围为30 ~ 60 nm。生物合成的AgNPs不仅具有较强的抗氧化活性,而且对人类致病菌具有较强的抗菌活性。AgNPs对MCF-7乳腺癌细胞系的细胞毒性研究也显示出剂量依赖性。合成的AgNPs具有公认的生物活性,可作为抗氧化、抗菌和细胞毒性药物用于临床。
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引用次数: 140
Wind characteristics observation using Doppler-SODAR for wind energy applications 利用多普勒- sodar进行风能特征观测
Pub Date : 2017-12-01 DOI: 10.1016/j.reffit.2017.07.001
Prem Kumar Chaurasiya, Siraj Ahmed, Vilas Warudkar

This paper presents an application of Doppler SODAR (Sound Detection and Ranging) system for the assessment of wind characteristics at an onshore site in Tamil Nadu, India. The wind speed is statistically analyzed by means of Weibull distribution function and results were used to compute several characteristics parameters related to wind energy applications and no significant discrepancies were observed. The characteristics of wind shear coefficient were evaluated for different altitudes. The vertical profile of wind speed measured from SODAR system was compared with existing models. Furthermore, the turbulence characteristics were analyzed and compared along with the turbulence intensity. From the economic point of view the SODAR system was found to be cost-effective at higher heights. The results of this study are expected to provide useful information for the deployment of remote sensing instruments for wind energy development in India.

本文介绍了多普勒SODAR(声音探测和测距)系统在印度泰米尔纳德邦陆上站点风特性评估中的应用。采用威布尔分布函数对风速进行统计分析,并将结果用于计算与风能应用相关的几个特征参数,没有发现显著差异。评价了不同高度的风切变系数特征。将SODAR系统测得的风速垂直廓线与现有模型进行了比较。此外,对湍流特性随湍流强度的变化进行了分析和比较。从经济角度来看,SODAR系统在较高的高度具有成本效益。预期这项研究的结果将为在印度部署用于风能开发的遥感仪器提供有用的资料。
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引用次数: 19
期刊
Resource-Efficient Technologies
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