Research in veterinary science最新文献

{"title":"From gut health to nutrient digestion and metabolism: An integrative biomarker analysis in nursery pigs","authors":"Marina P. Walter ,&nbsp;Gabriela M. Galli ,&nbsp;Marcos Kipper ,&nbsp;Karine L. Takeuti ,&nbsp;Ines Andretta","doi":"10.1016/j.rvsc.2026.106090","DOIUrl":"10.1016/j.rvsc.2026.106090","url":null,"abstract":"<div><div>This study aimed to explore the relationship between intestinal health biomarkers and nutrient digestion and metabolism in nursery pigs. Thirty-two weaned pigs were individually housed and fed a single experimental diet for 22 days. Feces and urine were collected on days 15 to 21 post-weaning to assess energy and protein digestibility and metabolizability. Blood samples were collected on day 22 to assess intestinal permeability, IgG, and IgA. Fecal samples were collected on the same day to quantify calprotectin, neopterin, lactoferrin, calcium-binding proteins, and fatty acid-binding protein (FABP). All pigs were euthanized on day 22 for intestinal tissue collection to determine villus height, villus width, crypt depth, and villus height-to-crypt depth ratio, thiobarbituric acid reactive substances (TBARS), glutathione S-transferase, protein thiols, resistance to rupture, zonula occludens-1, and occludin. Principal component analysis (PCA), correlation, and classification and regression tree (CART) methods were applied. Fecal calcium-binding proteins and FABP levels were identified in the tree-based models (CART) as key predictors of dry matter digestion. On the other hand, fecal calprotectin and FABP levels were the primary determinants of protein and energy digestibility and metabolizability. Fecal calprotectin levels between 79.4 and 79.6 ng/mL served as the cutoff to distinguish nursery pigs with high and low digestibility in two different CART models. Similarly, the cutoff for FABP levels ranged from 70.5 to 87.4 ng/mL, indicating its role in differentiating groups with high and low efficiency. Our results support the potential of fecal biomarkers as non-invasive indicators of digestibility and metabolism in weaned pigs.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106090"},"PeriodicalIF":1.8,"publicationDate":"2026-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146100554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cervical disc disease in whippets: Results of an online survey involving Italian owners and breeders","authors":"Diletta Dell'Apa ,&nbsp;Ezio Bianchi ,&nbsp;Luigi Auletta ,&nbsp;Mariaelena de Chiara ,&nbsp;Fabiana Micieli ,&nbsp;Francesco Ferrari ,&nbsp;Barbara Lamagna ,&nbsp;Maria Pia Pasolini","doi":"10.1016/j.rvsc.2026.106068","DOIUrl":"10.1016/j.rvsc.2026.106068","url":null,"abstract":"<div><div>Whippet is considered quite a healthy breed. Nonetheless, the perception of some veterinary neurologists is that the occurrence of neurological diseases, especially cervical disc disease (CDD), is frequent in this breed. This study aimed to get information about the prevalence of the most common disorders and conditions affecting the Italian Whippet population, particularly investigating the prevalence of CDD and the perception of the problem by owners and breeders. Owners and breeders included in the Italian Sighthound Club register were invited to answer anonymously an online questionnaire. Descriptive statistics were used for breeder/owner and dog characteristics, and statistical association with the development of CDD were explored. Over 280 Whippets, the overall prevalence of CDD was 7.2% and 29% in Whippets older than 9 years. Whippets older than 9 years were overrepresented in the CDD group (<em>P</em> &lt; 0.0001; O.R. 0.07; 95% CI: 0.02–0.22) compared with the CDD group. These findings confirm the hypothesis that CDD may be a challenging and more prevalent than expected disorder in this breed and health screening programs could be recommended to breeders and owners to assess cervical spine conformation, especially before sporting activity or breeding.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106068"},"PeriodicalIF":1.8,"publicationDate":"2026-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146081421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in expression of growth, immune activity and adaptation related genes in heat stressed dorper and second cross lambs","authors":"Aleena Joy ,&nbsp;Swagat Mohapatra ,&nbsp;Frank R. Dunshea ,&nbsp;Brian J. Leury ,&nbsp;Kristy DiGiacomo ,&nbsp;Minghao H. Zhang ,&nbsp;Archana Abhijith ,&nbsp;Richard Osei- Amponsah ,&nbsp;Iain J. Clarke ,&nbsp;Surinder Singh Chauhan","doi":"10.1016/j.rvsc.2026.106087","DOIUrl":"10.1016/j.rvsc.2026.106087","url":null,"abstract":"","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106087"},"PeriodicalIF":1.8,"publicationDate":"2026-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146081420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Minimally invasive glove port hernioscopy in a female dog with an intermittent hernia and abdominal mass: A case report","authors":"Catherine Konrad Nava Calva ,&nbsp;Amanda Oliveira Paraguassú ,&nbsp;Anna Vitória Hörbe ,&nbsp;Felipe Auatt Batista de Sousa ,&nbsp;Otávio Henrique de Melo Schiefler ,&nbsp;Layla de Jesus Neri Cerqueira ,&nbsp;Luna Silvestri Souto ,&nbsp;Felipe Augusto Ruiz Sueiro ,&nbsp;Andrigo Barboza de Nardi ,&nbsp;Maurício Veloso Brun","doi":"10.1016/j.rvsc.2026.106086","DOIUrl":"10.1016/j.rvsc.2026.106086","url":null,"abstract":"<div><div>Minimally invasive surgery in small animals is constantly advancing, reducing tissue trauma, and improving diagnostic accuracy. This report documented the first use of hernioscopy associated with the glove port technique in a female dog with an partially reducible inguinal hernia. The 11-year-old patient exhibited a right inguinal swelling containing intestinal loops, concomitant with a extensive cystic intra-abdominal mass. An exploratory laparoscopy was performed through the inguinal hernia utilizing the glove port technique to establish multiport access without muscle wall incision. This method minimized tissue trauma and enabled comprehensive evaluation of the abdominal cavity, as well as partial mass resection. The procedure offered palliative benefits and introduced a novel application of laparoscopy for managing inguinal hernias in dogs.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"202 ","pages":"Article 106086"},"PeriodicalIF":1.8,"publicationDate":"2026-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146039741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenylalanine as a biomarker of disease in animals: Current evidence and future perspectives","authors":"James Mettam ,&nbsp;Nauman Zaheer Ghumman ,&nbsp;Bao-Tao Liu ,&nbsp;Henry Annandale ,&nbsp;Jully Gogoi-Tiwari","doi":"10.1016/j.rvsc.2026.106066","DOIUrl":"10.1016/j.rvsc.2026.106066","url":null,"abstract":"<div><div>Phenylalanine (Phe), an essential aromatic amino acid, is emerging as a potential biomarker of metabolic and health status in animals. Altered Phe concentrations reflect changes in metabolic, inflammatory, and disease processes. In companion animals, shifts in serum or biofluid Phe have been linked to hepatopathies, inflammatory enteropathies, and neoplastic conditions. In livestock, variations in milk or serum Phe accompany mastitis, metabolic stress, lameness, and other production-related disorders. Evidence for cats, horses, poultry, and aquaculture species remains limited. Interpretation is complicated by analytical variability, small sample sizes, and confounding factors such as diet, stress, and microbiome composition.</div><div>Non-invasive sampling of milk, urine, saliva, and breath, shows promise for Phe monitoring across animal species. Advances in multi-omics, particularly metabolomics, proteomics, and microbiome analyses, can clarify mechanisms and support development of composite biomarker panels. This review evaluates current evidence on Phe as a biomarker across species, highlights gaps in research coverage and methodology, and outlines priorities for future work. Expanding studies to underrepresented species, standardizing measurement protocols, and conducting longitudinal research. Addressing these priorities is essential to establish Phe as a robust biomarker, for veterinary diagnostics, health monitoring, and the optimization of animal welfare and production systems.</div></div><div><h3>Short summary</h3><div>This review looks at phenylalanine (Phe), an amino acid, as a possible health indicator in animals. Changes in Phe levels may show problems with metabolism, inflammation, or disease. Evidence is strongest for dogs and dairy cows, but limited for cats, horses, poultry, and fish. Results vary due to measurement methods and other factors. Future studies should standardize testing, include more species, and combine methods to improve Phe's usefulness in monitoring animal health.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"202 ","pages":"Article 106066"},"PeriodicalIF":1.8,"publicationDate":"2026-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146039743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of fresh frozen plasma immunotherapy as an adjuvant treatment of neonatal sepsis in puppies","authors":"Keylla Helena Nobre Pacífico Pereira ,&nbsp;Kárita da Mata Fuchs ,&nbsp;Gleice Mendes Xavier ,&nbsp;Júlia Cosenza Mendonça ,&nbsp;Diogo Ribeiro Câmara ,&nbsp;Luiz Eduardo Cruz dos Santos Correia ,&nbsp;Paulo Fernandes Marcusso ,&nbsp;Fabiana Ferreira de Souza ,&nbsp;Márcio Garcia Ribeiro ,&nbsp;Maria Lucia Gomes Lourenço","doi":"10.1016/j.rvsc.2026.106085","DOIUrl":"10.1016/j.rvsc.2026.106085","url":null,"abstract":"<div><div>The aim of this study was to evaluate the effects of fresh frozen plasma (passive immunotherapy) on clinical and laboratorial findings in puppies treated for neonatal sepsis. A total of thirty-one puppies were included in this study, 16 were diagnosed with sepsis and treated either with plasma + antibiotic (plasma group, <em>n</em> = 7; PG) or antibiotic only (control group, <em>n</em> = 9; CG); whereas 15 were healthy neonates (HG). The groups were evaluated at the first appointment (M0), after 24 (M24), 48 (M48), 72 h (M72) and at the end of treatment (ME). At M0, neonates with sepsis presented a reduction (<em>p</em> &lt; 0.05) in heart rate (HR), reflexes, blood glucose, body temperature, and leukocytes (leukopenia), with an increase (p &lt; 0.05) in lactate and troponin I, compared to HG puppies. During treatment, significant improvement of laboratory parameters was observed in PG compared to CG puppies, with higher IgM level at M24, and higher blood glucose and leukocyte count at M24 and M48 (<em>p</em> &lt; 0.05). At ME, the lactate was lower in PG than CG puppies (p &lt; 0.05). There were no differences in the serum IgG or IgA concentrations among groups. Evaluating clinical and laboratory improvement between time points within the same group, differences (<em>p</em> &lt; 0.05) were observed in the PG puppies, which increased blood glucose, temperature, leukocytes count, and IgM at M24 compared to M0. In the CG, a decrease in IgM concentration was detected at M24 compared to M0 (<em>p</em> &lt; 0.05). Approximately 57% (4/7) of the neonates in the PG presented leukograms without alterations at M24. In the CG, leukograms without alterations were observed only at M72 in 55.5% (5/9) of the animals. The mortality rate was 22% (2/9) in the CG. There was no mortality in the PG. This was the first study using fresh frozen plasma as an adjuvant in the treatment of neonatal sepsis in dogs and evaluation of biomarkers in diagnosis. It was concluded that sepsis led to changes in the physiological parameters of neonatal dogs, demonstrating that the evaluation of leukocytes, troponin I and lactate can be used in the diagnosis and monitoring of sepsis. It was concluded that, besides being an important source of IgM, fresh frozen plasma stimulated healing of puppies with neonatal sepsis, by prompt (within 24 h) improvement in both clinical and laboratory parameters. Plasma therapy has been shown to be beneficial in the treatment of sepsis and is a promising therapeutic strategy for reducing neonatal mortality.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"202 ","pages":"Article 106085"},"PeriodicalIF":1.8,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146039742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluated the immune efficacy of a bivalent inactivated vaccine against post-weaning multisystemic wasting syndrome and mycoplasmal pneumonia of swine","authors":"Jianli Shi ,&nbsp;Chang Liu ,&nbsp;Xiaoyan Wu ,&nbsp;Yongming Wang ,&nbsp;Chen Li ,&nbsp;Jun Li","doi":"10.1016/j.rvsc.2026.106076","DOIUrl":"10.1016/j.rvsc.2026.106076","url":null,"abstract":"<div><div>PMWS and MPS are severe respiratory diseases in piglets, causing developmental disorders and significant economic losses. The conventional approach to control these diseases relies on separate immunization with individual vaccines, which is time-consuming, labor-intensive, and may induce stress reactions. The aim of this study was to evaluate the immune efficacy of a bivalent inactivated vaccine containing the clinically isolated Mhp Q strain and purified PCV2 Cap VLPs. Thirty-five Changbai piglets were randomly divided into seven groups (<em>n</em> = 5, per group): A (bivalent inactivated vaccine with PCV2 challenged), B (bivalent inactivated vaccine with Mhp challenged), C (commercial PCV2 vaccine with PCV2 challenged), D (commercial Mps vaccine with Mhp challenged), E (sham-vaccinated with PCV2 challenged), F (sham-vaccinated with Mhp challenged), and G (sham-vaccinated with sham challenged). The immune efficacy results demonstrated that 5/5 protection with bivalent inactivated vaccine and 4/5 protection with commercial vaccine against PCV2 challenge, which was consistent with the serology results. The pneumonia lesion scores result demonstrated that the immune protection effect provided by the bivalent inactivated vaccine was comparable to that of the commercial Mps vaccines. Furthermore, the overall clinical trial results indicated that the bivalent inactivated vaccine was safe and controllable, with no adverse reactions observed in both age-matched and non-age-matched pigs. Following immunization, the pigs exhibited significant resistance to infections with both PCV2 and Mhp, reflecting a robust immunoprotective capacity. Collectively, these results support the bivalent inactivated vaccine as a safe and effective strategy for preventing and controlling PMWS and MPS, with promising potential for further clinical development.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"202 ","pages":"Article 106076"},"PeriodicalIF":1.8,"publicationDate":"2026-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146039304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiparasitic practices in Portuguese animal shelters: A survey of veterinarians","authors":"Ana Luísa Martins ,&nbsp;Joana Mourão ,&nbsp;Irina Amorim ,&nbsp;Luís Cardoso ,&nbsp;João Rodrigo Mesquita","doi":"10.1016/j.rvsc.2026.106073","DOIUrl":"10.1016/j.rvsc.2026.106073","url":null,"abstract":"<div><div>Animal shelters often operate under significant resource constraints while caring for large populations of dogs and cats, many of whom are affected by parasitic infections that impact animal health and pose zoonotic risks. These limitations hinder the implementation of standardized, evidence-based parasite control protocols. This study surveyed veterinarians working in Portuguese shelters to describe current antiparasitic treatment practices and identify key limitations. A structured questionnaire was distributed to veterinarians in shelters or kennels affiliated with ICBAS, University of Porto, collecting data on animal populations, location, diagnostic testing, and antiparasitic drug use. A total of 41 responses were analysed. Additionally, a cost analysis was performed using 2024 wholesale antiparasitic treatment prices, based on average weights (20 kg dogs, 5 kg cats). Commercially available products were reviewed for active ingredients, target parasites, and cost per treatment. Descriptive statistics were used to summarize responses; no inferential analyses were performed given the limited sample size. Ectoparasiticides were the most frequently used treatments, followed by broad-spectrum nematocides and cestocides. Only 12% of shelters reported conducting diagnostic testing prior to treatment. Drug selection was primarily influenced by cost and perceived efficacy. Commonly used compounds included praziquantel, pyrantel, fipronil, fenbendazole, and milbemycin oxime, several of which have reported resistance in parasite populations. Treatment costs ranged from €0.82 to €219 per dose for 20 kg dogs and €0.73 to €34.84 for 5 kg cats. The limited use of diagnostics and reliance on broad-spectrum drugs reflect a reactive rather than preventative approach. Findings underscore the need for improved diagnostic protocols and strategic drug selection to enhance parasite control and animal welfare.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"202 ","pages":"Article 106073"},"PeriodicalIF":1.8,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146039302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure of the nucleoli in the spermatocytes of three canid species","authors":"Katarzyna Andraszek ,&nbsp;Marta Kuchta-Gładysz ,&nbsp;Piotr Niedbała","doi":"10.1016/j.rvsc.2026.106063","DOIUrl":"10.1016/j.rvsc.2026.106063","url":null,"abstract":"<div><div>The nucleolus is a structure of the cell nucleus associated mainly with ribosome biogenesis and indirectly responsible for protein biosynthesis. The nucleolus also plays a role in the regulation of cell growth and cell cycle progression, the detection and repair of DNA damage, and the export or degradation of mRNA. It is also involved in the most critical processes for the organism, such as ageing at the cellular level and broadly defined carcinogenesis. Analysis of the structure of nucleoli, which are the products of nucleolar organizer regions, can be an alternative source of information on the activity of rRNA-encoding genes. The material for the study was testes collected post mortem from sexually mature males of three canid species: silver fox (<em>Vulpes vulpes</em>), blue fox (<em>Alopex lagopus</em>) and Chinese raccoon dog (<em>Nyctereutes procyonoides procyonoides</em>). The morphology and morphometry of nucleoli identified in early primary spermatocytes were analysed. The values for all morphometric parameters of the spermatocyte nucleus were lowest for the silver fox and highest for the raccoon dog. In the case of nucleolus parameters, the lowest values were found in the raccoon dog and the lowest in the blue fox. The size of the nucleoli and spermatocyte nucleus proved to be a species-specific trait associated with the karyotype and number of nucleolar organizer regions in the species. The nucleolus is believed to play an important role in controlling meiosis. It is involved in preventing chromosome segregation during crossing over, and proteins associated with the nucleoli identify damaged synaptonemal complexes. Epigenetic regulations at the level of the nucleolus and rRNA genes remain a crucial subject of genetic research.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"202 ","pages":"Article 106063"},"PeriodicalIF":1.8,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146003305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological factors of Eucoleus aerophilus infection in red foxes (Vulpes vulpes) from semi-arid Mediterranean environments","authors":"I. Arcenillas-Hernández ,&nbsp;M. Català-Barrasetas ,&nbsp;P. Pérez-Cutillas ,&nbsp;M.R. Ruiz de Ybáñez ,&nbsp;C. Martínez-Carrasco","doi":"10.1016/j.rvsc.2026.106064","DOIUrl":"10.1016/j.rvsc.2026.106064","url":null,"abstract":"<div><div><em>Eucoleus aerophilus</em> is a ubiquitous zoonotic nematode found in the tracheobronchial mucosa of the definitive hosts, described in wild and domestic canids, including the red fox (<em>Vulpes vulpes</em>). To assess <em>E. aerophilus</em> occurrence and intensity in red foxes, the respiratory system of 126 foxes from Region of Murcia (SE, Spain) were examined. Statistical analyses were performed to investigate the influence of biotic and abiotic factors on the parasite's occurrence. The occurrence of <em>E. aerophilus</em> was 28.6% (95% CI: 20.6–36.4), with a mean intensity of 3.1 nematodes per parasitised fox. The infection rate and parasite intensity were higher in forested areas, suggesting that earthworm abundance and a longer survival of infective <em>E. aerophilus</em> eggs increase the risk of transmission and maintenance in the environment. Moreover, an inverse relationship between <em>E. aerophilus</em> occurrence and temperature (<em>p</em> &lt; 0.05) was observed. Results demonstrated that foxes participate in maintaining the cycle of <em>E. aerophilus</em> in semi-arid Mediterranean environments. This should be taken into account at the wild-domestic-human interface. The development of a predictive model of <em>E. aerophilus</em> infection risk in red foxes will allow the design of future strategies for the prevention of capillariosis, especially in areas where contact between foxes, domestic carnivores, and humans exists.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"202 ","pages":"Article 106064"},"PeriodicalIF":1.8,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146012034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}