Research in veterinary science最新文献_第2页

Interleukin-6 promotes the epithelial mesenchymal transition in canine tonsillar squamous cell carcinoma cells 白细胞介素-6促进犬扁桃体鳞状细胞癌细胞上皮间质转化。

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-02-01 DOI: 10.1016/j.rvsc.2024.105487

Shunsuke Noguchi , Ryo Shimonishi

Canine oral squamous cell carcinoma (CoSCC) is often associated with suppurative inflammation. Metastasis of malignant tumors is one of the signs of major interest in oncology because it speaks of disease progression, where the involvement of interleukin-6 (IL-6) in cancer progression is so far unknown. Therefore, the aim of this study was the determination of the role of IL-6 in metastasis in CoSCC cells model through expression analysis of mRNA and protein using real-time PCR and western blotting and assessment of cell migration and invasion. The messenger RNA (mRNA) expression level of IL-6 was elevated in CoSCC tissues, and the IL-6 receptor protein was expressed in CoSCC cell lines. Furthermore, IL-6 levels were associated and showed negative correlation with survival time (rs = −0.92857) in dogs with tonsillar SCC. Recombinant canine IL-6 (rcIL-6) treatment promoted migration and invasion, in addition to increasing the viable cell number of the tonsillar SCC cell line (TSCCLN#6). Consistently, the protein expression of phosphorylated ERK1/2 and STAT3 and Fascin1 (FSCN1) was upregulated by treatment with rcIL-6 in a dose-dependent manner. Treatment with ERK or STAT3 inhibitors abolished the effects of rcIL-6, and the ERK inhibitor successfully downregulated the expression of FSCN1. In conclusion, IL-6 may be involved in tonsillar CoSCC invasion and metastasis through the activation of the mitogen-activated protein kinase and Janus tyrosine kinase/signal transducer and activator of transcription signaling.

犬口腔鳞状细胞癌（CoSCC）通常与化脓性炎症有关。恶性肿瘤的转移是肿瘤学的主要兴趣之一，因为它说明了疾病的进展，其中白介素-6 （IL-6）在癌症进展中的参与迄今尚不清楚。因此，本研究的目的是通过real-time PCR和western blotting分析mRNA和蛋白的表达，评估细胞迁移和侵袭，确定IL-6在CoSCC细胞模型转移中的作用。IL-6信使RNA （mRNA）在CoSCC组织中表达水平升高，IL-6受体蛋白在CoSCC细胞系中表达。此外，扁桃体鳞状细胞癌患者IL-6水平与生存时间呈负相关（rs = -0.92857）。重组犬IL-6 （rcIL-6）处理除了增加扁桃体SCC细胞系（tsccln# 6）的活细胞数量外，还促进了迁移和侵袭。与此一致的是，磷酸化的ERK1/2、STAT3和Fascin1 （FSCN1）的蛋白表达在rcIL-6的作用下呈剂量依赖性上调。用ERK或STAT3抑制剂治疗可消除rcIL-6的作用，ERK抑制剂成功下调FSCN1的表达。综上所述，IL-6可能通过激活丝裂原激活蛋白激酶和Janus酪氨酸激酶/信号转导和转录信号激活因子参与扁桃体CoSCC的侵袭转移。

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引用次数: 0

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-02-01 DOI: 10.1016/j.rvsc.2024.105498

Barbara Padalino , Francesco Pellegrini , Martina Felici , Martina Zappaterra , Cristiana Catella , Marco Cordisco , Eleonora Lorusso , Francesco Cirone , Annamaria Pratelli

Respiratory disorders (RDs) are a common transport-related welfare consequence in horses. This prospective study described the journeys of horses transported to two slaughterhouses in Italy, documented the prevalence of potentially RD-related pathogens, and identified possible predisposing factors. Data were recorded from arrival until 48 h after arrival and included the collection of Deep Nasopharyngeal Swabs (DNS), journey details and welfare assessment of horses (n = 155). PCR was used to quantify the presence of influenza A virus, reovirus, equine herpesvirus type 1 and 4, adenovirus, rhinovirus, astrovirus, equine coronavirus, Pasteurellaceae, Streptococcus equi sub. equi and sub. zooepidemicus. One horse each tested positive for reovirus, and rhinovirus and two horses tested positive for adenovirus. The prevalence of Streptococcus equi and zooepidemicus and Pasteurellaceae was 4 %, 19 % and 63 % respectively. Streptococcaceae and Pasteurellaceae were associated with sweating (P < 0.001). In addition, Streptococcaceae was associated with abnormal demeanour (P = 0.003), nasal or ocular discharge (P < 0.001) and higher compromised welfare after transport (P < 0.001). The multivariable regression analysis showed that broken horses departing from Eastern Europe or France were more likely to test positive for Streptococcaceae than unbroken horses coming from Poland (model P < 0.001), while broken horses subjected to short stops en route were more likely to test positive for Pasteurellaceae than unbroken horses on journeys without stops (model P < 0.001). Our findings increase the understanding of predisposing factors for RDs and may be useful in the implementation of regulations to protect the welfare of horse during transport.

呼吸道疾病（rd）是马常见的运输相关的福利后果。这项前瞻性研究描述了运往意大利两个屠宰场的马的旅程，记录了可能与rd相关的病原体的流行情况，并确定了可能的诱发因素。数据记录从到达到到达后48小时，包括收集深鼻咽拭子（DNS），行程细节和马的福利评估（n = 155）。采用PCR定量检测甲型流感病毒、呼肠孤病毒、马1型和4型疱疹病毒、腺病毒、鼻病毒、星状病毒、马冠状病毒、巴氏杆菌、马链球菌亚种和动物流行病亚种的存在情况。每匹马的呼肠孤病毒和鼻病毒检测呈阳性，两匹马的腺病毒检测呈阳性。马链球菌、动物流行病和巴氏杆菌的检出率分别为4%、19%和63%。链球菌科和巴氏杆菌科与发汗有关(P

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引用次数: 0

Genetic characterization of infectious bursal disease virus strains with distinct VP2 amino acid profiles emerging in Pakistan 巴基斯坦出现的具有不同VP2氨基酸谱的传染性法氏囊病病毒株的遗传特征

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-02-01 DOI: 10.1016/j.rvsc.2024.105509

Sanaullah Sajid , Haseeb Ullah Sajid

Infectious bursal disease (IBDV) poses a significant threat to the global poultry industry and causes major economic losses. This study presents the genetic profile of IBDV strains emerging in Pakistan, focusing on the VP2 amino acid profile. The effects of these changes on disease transmission, vaccine effectiveness, and overall chicken health are concerning. A meticulous analysis was carried out by isolating and clustering IBDV strains collected from Faisalabad district of Pakistan. The genetics of these viruses were evaluated by analyzing the VP2 gene, the main component of the virus. Importantly, our results reveal the existence of IBDV strains with unprecedented VP2 amino acid profiles, indicating genetic changes that may affect virulence and immunity. This study highlighted the changing IBDV landscape in Pakistan and underscores the importance of continued research and surveillance efforts. Understanding the genetic structure of these emerging diseases is essential for developing effective control strategies, including vaccine development and control. This also emphasized the need for international cooperation to reduce the spread of the new IBDV strains, which could significantly impact the poultry industry outside Pakistan.

传染性法氏囊病（IBDV）对全球家禽业构成重大威胁，并造成重大经济损失。本研究介绍了在巴基斯坦出现的IBDV菌株的遗传谱，重点是VP2氨基酸谱。这些变化对疾病传播、疫苗有效性和整体鸡健康的影响令人担忧。通过分离和聚类收集自巴基斯坦费萨拉巴德地区的IBDV菌株，进行了细致的分析。通过分析病毒的主要成分VP2基因来评价这些病毒的遗传学。重要的是，我们的研究结果揭示了IBDV菌株存在前所未有的VP2氨基酸谱，表明遗传变化可能影响毒力和免疫。这项研究强调了巴基斯坦IBDV格局的变化，并强调了继续开展研究和监测工作的重要性。了解这些新发疾病的遗传结构对于制定有效的控制战略，包括疫苗开发和控制至关重要。这也强调需要进行国际合作，以减少新的IBDV毒株的传播，这可能对巴基斯坦以外的家禽业产生重大影响。

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引用次数: 0

Efficacy of transdermal ketoprofen on surgical inflammation in dogs 透皮酮洛芬治疗犬外科炎症的疗效。

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-02-01 DOI: 10.1016/j.rvsc.2024.105512

H.G. Ravuri , P. Sadowski , N. Satake , P.C. Mills

Ketoprofen is a non-steroidal anti-inflammatory drug (NSAID) used to treat pain and inflammation in dogs. Despite having effective analgesic efficacy, prolonged oral administration has been associated with adverse effects. Transdermal delivery of ketoprofen has reduced the incidence of adverse effects in humans and could potentially be used in veterinary clinical medicine. A transdermal (TD) ketoprofen formulation was recently developed for use in dogs and this study aimed to determine the pharmacodynamic activity of this formulation using surgical castration as an acute inflammatory model. Twelve dogs were randomly assigned to either a Control group (n = 6) or a TD group (n = 6). All dogs were castrated using standard surgical protocols, administered with pre-medication, consisting of acepromazine (0.055 mg/kg) and methadone (0.5 mg/kg) intramuscularly (IM) 30 min prior to induction of general anaesthesia. All dogs were then anaesthetised by injecting alfaxalone (2 mg/kg IV) via a 20 G 3 cm catheter in the left cephalic vein and subsequently maintained using isoflurane. Along with that TD group dogs also received TD ketoprofen (10 mg/kg) 1 h before pre-medication. Bloods were collected at 0 – hour (pre-surgery), and 1 and 2-h post-surgery and analysed for circulating eicosanoids using liquid chromatography mass spectrometry (LCMS) methods. Measured levels of Thromboxane B₂ (TXB₂) at both 1 and 2 h and Prostaglandin E₂ (PGE₂) at 2 h post-surgery were higher in the Control group compared to the TD group, suggesting pre-operative application of TD ketoprofen has a possible inhibitory effect on systemic inflammation and could be used to treat pain and inflammation in dogs.

酮洛芬是一种非甾体抗炎药（NSAID），用于治疗狗的疼痛和炎症。尽管具有有效的镇痛效果，但长期口服已与不良反应有关。经皮给药酮洛芬降低了人类不良反应的发生率，并有可能用于兽医临床医学。最近开发了一种透皮（TD）酮洛芬制剂，用于狗，本研究旨在通过手术阉割作为急性炎症模型确定该制剂的药效学活性。12只狗随机分为对照组（n = 6）和TD组（n = 6）。所有狗都采用标准手术方案阉割，在全身麻醉前30分钟肌肉注射乙酰丙嗪（0.055 mg/kg）和美沙酮（0.5 mg/kg）。所有犬均通过20 G 3 cm的左头静脉导管注射阿法沙龙（2mg /kg IV）麻醉，随后使用异氟烷维持麻醉。与TD组狗一起，在用药前1小时给予TD酮洛芬（10 mg/kg）。术前0小时、术后1小时和2小时采集血液，采用液相色谱-质谱法（LCMS）分析循环类二十烷酸。对照组术后1和2小时血栓素B2 （TXB2）和前列腺素E2 （PGE2）水平均高于TD组，提示术前应用TD酮洛芬可能对全身炎症有抑制作用，可用于治疗犬的疼痛和炎症。

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引用次数: 0

Evaluation of Scalibor® deltamethrin collar chronic exposure in dogs 犬慢性接触Scalibor®溴氰菊酯项圈的评价。

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-02-01 DOI: 10.1016/j.rvsc.2024.105502

Larissa T.P. Rodrigues , Andriele F. Qualhato , Carolle V.M. Sprícigo , Kelly C.A. Arantes , Rayanne H.S. da Silva , Alexandre C.M. Barros , Monica C. Vicente , Murilo R. Souza , Luiza G.F. de Paula , Danilo Roman-Campos , Artur S. Miranda , Danieli B. Martins , Ana F.M. Botelho

Deltamethrin is a pesticide used worldwide with several applications in the control of nuisance arthropods in homes, agriculture, against ectoparasites in dogs and livestock. Recent studies indicate that deltamethrin can induce cardiotoxicity. Therefore, this study aimed to evaluate the toxicity of deltamethrin in dogs through exposure to commercial collars over 8 months. Twenty healthy dogs were selected, 9 males and 11 females, weighing between 10 and 15 kg and aged between 1 and 8 years, with no history of intentional exposure to pyrethroids. Clinical, cardiological, and laboratory evaluations were performed monthly. The results demonstrate that exposure to deltamethrin 4 % via the dermal route, through a commercial collar, was safe for 8 months, with preserved cardiac electrocontractile function, assessed by electrocardiogram and echocardiogram. In addition, tracking of plasma biochemical markers did not indicate signs of cardiac, renal, or hepatic injury. Although deltamethrin 4 % collars demonstrated an acceptable safety profile regarding cardiovascular and laboratory evaluations, the occurrence of dermatological reactions in 25 % of the animals, which is a significant proportion, highlights the need for careful monitoring of dermatological reactions. Future investigations with different concentrations and longer periods are recommended to achieve a more comprehensive understanding of the long-term effects of deltamethrin, improving its safe application in veterinary medicine and promoting animal and human health.

溴氰菊酯是一种世界范围内使用的杀虫剂，用于控制家庭、农业中的有害节肢动物，以及防止狗和牲畜中的体外寄生虫。近年来的研究表明，溴氰菊酯可引起心脏毒性。因此，本研究旨在通过暴露于商业项圈8个月以上来评估溴氰菊酯对狗的毒性。选取了20只健康犬，雄性9只，雌性11只，体重在10至15公斤之间，年龄在1至8岁之间，没有故意接触拟除虫菊酯的历史。每月进行临床、心脏科和实验室评估。通过心电图和超声心动图评估，结果表明，经皮肤途径，通过商业项圈暴露于4%溴氰菊酯8个月是安全的，心脏电收缩功能得以保存。此外，血浆生化标记物的追踪没有显示心脏、肾脏或肝脏损伤的迹象。尽管4%溴氰菊酯在心血管和实验室评估方面显示出可接受的安全性，但在25%的动物中发生了皮肤病反应，这是一个很大的比例，强调了仔细监测皮肤病反应的必要性。建议今后开展不同浓度和较长时间的调查，以更全面地了解溴氰菊酯的长期影响，提高其在兽药中的安全应用，促进动物和人类健康。

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引用次数: 0

Global prevalence of lagomorpha coccidiosis from 1951 to 2024: A systematic review and meta-analysis 1951 - 2024年lagomorpha球虫病全球流行情况：系统回顾和荟萃分析。

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-02-01 DOI: 10.1016/j.rvsc.2024.105519

Huang-Liang Chen, Qi-Tao Zhang, Rui Qi

The global prevalence of coccidia infection in lagomorphs and potential risk factors were investigated through a meta-analysis of 149 studies published between 1951 and 2024. The pooled prevalence of Eimeriidae, Sarcocystidae and Cryptosporidiidae was found to be 66.0 %, 8.0 % and 6.1 %, respectively. Rabbits and hares exhibited the highest prevalence of Eimeriidae at 63.1 % and 71.8 %, while pikas had the highest prevalence of Cryptosporidiidae at 17.1 %. Countries located in the southern hemisphere exhibited the highest prevalence of Cryptosporidiidae (11.4 %). The African Region had the highest prevalence of Eimeriidae (73.3 %) and Sarcocystidae(14.6 %), while the Eastern Mediterranean Region had the highest prevalence of Cryptosporidiidae(13.6 %). Juvenile rabbits exhibited the highest prevalence of Eimeriidae (67.4 %), while senior Rabbit exhibited the highest prevalence of Sarcocystidae(17.1 %) and Cryptosporidiidae(13.1 %). The prevalence of coccidia in intensively farmed lagomorphs showed a slight upward trend over time, nearly reaching statistical significance (R2 = 7.88 %, coefficient = 0.019, P = 0.127). This suggests that the current intensive farming practices may be losing effectiveness in controlling coccidia infections, which is concerning. Among Eimeriidae in lagomorphs, Eimeria coquelinae showed the highest prevalence (68.4 %), while E. magna was most frequently reported. For Sarcocystidae, Besnoitia spp. had the highest prevalence (31.3 %), with Toxoplasma gondii being most frequently reported. Cryptosporidium cuniculus was both the most prevalent and frequently reported Cryptosporidiidae species (6.9 %), suggesting it may be a major Cryptosporidiidae pathogen in lagomorphs. These findings highlight the differences in prevalence of various Eimeriida species among different lagomorpha genera. This diversity underscores the complex relationships between specific parasites and their host species, contributing to a more nuanced understanding of coccidiosis epidemiology in lagomorphs worldwide.

通过对1951年至2024年间发表的149项研究的荟萃分析，调查了狐猴球虫感染的全球流行情况及其潜在危险因素。其中艾虫科、肉囊虫科和隐孢子虫科的总流行率分别为66.0%、8.0%和6.1%。家兔和野兔的隐孢子虫科感染率最高，分别为63.1%和71.8%，鼠兔的隐孢子虫科感染率最高，为17.1%。南半球隐孢子虫科的流行率最高（11.4%）。非洲区艾扁虫科（73.3%）和肉囊虫科（14.6%）感染率最高，东地中海区隐孢子虫科（13.6%）感染率最高。雏兔艾虫科患病率最高（67.4%），成年兔肉囊虫科患病率最高（17.1%），隐孢子虫科患病率最高（13.1%）。随着时间的推移，集约化养殖lagomorphi的球虫感染率呈轻微上升趋势，接近具有统计学意义（R2 = 7.88%，系数= 0.019,P = 0.127）。这表明目前的集约化养殖方法可能正在失去控制球虫感染的有效性，这是令人担忧的。在狐形目艾美球虫科中，coquelin艾美球虫的感染率最高（68.4%），而magna艾美球虫的感染率最高。在肉囊虫科中，以贝氏虫最高（31.3%），以刚地弓形虫最常见。隐孢子虫是隐孢子虫科最常见和最常报道的一种（6.9%），提示其可能是狐形目隐孢子虫科的主要病原体。这些发现突出了艾美拉属中不同种类艾美拉属的流行率差异。这种多样性强调了特定寄生虫与其宿主物种之间的复杂关系，有助于更细致地了解世界范围内lagomorphi的球虫病流行病学。

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引用次数: 0

Survey on canine hookworms: Knowledge, perceptions and practices among U.S. veterinarians 犬钩虫调查：美国兽医的知识、观念和实践。

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-02-01 DOI: 10.1016/j.rvsc.2024.105501

Sara J. Ochoa , Gregg A. Hanzlicek , Kamilyah R. Miller , Justin Kastner , Jeba R.J. Jesudoss Chelladurai

Canine hookworms (Ancylostoma caninum) are highly prevalent in pet dogs in the United States of America (U.S.). They pose a significant health risk to infected dogs and are also a potential risk to humans as zoonotic agents. Veterinarians play a crucial role in managing this threat through the diagnosis and treatment of infections in pet dogs. However, the rising incidence of anthelmintic resistance in U.S. hookworm populations necessitates increased awareness among veterinarians. To determine U.S. veterinarian's knowledge, perceptions and practices about canine hookworms, a cross-sectional online survey was administered between February and December 2022. 208 veterinarians from 43 states participated in the study. Only 65 % of veterinarians reported awareness of hookworm prevalence. Hookworm infected dogs were encountered more frequently in the South than in Western states. Veterinarians perceived that hookworms were present in all breeds without breed predilection followed by mixed breeds, pit bulls and hound breeds. Most respondents reported that infections were rarely symptomatic, highlighting the importance of diagnostic testing. In-clinic diagnostic modalities were most preferred. Fenbendazole and pyrantel pamoate were the most commonly used anthelmintics. Most veterinarians followed up on infected dogs but retesting time after treatment varied. Awareness of multi-drug resistant hookworms was high but encountering persistent hookworm cases was lower. Veterinarians frequently educated clients about hookworms and prevention, but less about the zoonotic potential. This study provides insights for improving awareness, diagnostics, treatment protocols, and client education regarding canine hookworms in the U.S.. Overall, veterinarians play an active role in hookworm prevention and control.

犬钩虫（Ancylostoma caninum）在美国的宠物狗中非常普遍。它们对受感染的狗构成重大健康风险，也作为人畜共患病病原体对人类构成潜在风险。兽医通过诊断和治疗宠物狗的感染，在管理这一威胁方面发挥着至关重要的作用。然而，在美国钩虫种群中，越来越多的抗虫率需要提高兽医的意识。为了确定美国兽医对犬钩虫的知识、认知和做法，在2022年2月至12月期间进行了一项横断面在线调查。来自43个州的208名兽医参与了这项研究。只有65%的兽医报告了解钩虫的流行。受钩虫感染的狗在南方比在西部各州更常见。兽医认为钩虫存在于所有品种中，没有品种偏好，其次是混合品种，比特犬和猎犬品种。大多数答复者报告说，感染很少有症状，这突出了诊断检测的重要性。临床诊断方式是首选。芬苯达唑和吡喃酯是最常用的驱虫药。大多数兽医对受感染的狗进行了随访，但治疗后重新检测的时间各不相同。对多重耐药钩虫的认识较高，但遇到持续性钩虫的病例较少。兽医经常教育客户关于钩虫和预防，但很少关于人畜共患的可能性。本研究为提高美国犬钩虫的认识、诊断、治疗方案和客户教育提供了见解。总之，兽医在钩虫的预防和控制中发挥着积极的作用。

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引用次数: 0

Differential expression of prostatic androgen receptor and alpha-smooth muscle actin in castrated and non-castrated dogs

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-02-01 DOI: 10.1016/j.rvsc.2025.105563

Sameh M. Farouk , Amal A.M. Ahmed , Mohamed A. Hashem , Safa H. Badran

The prostate, the only accessory sex gland in dogs, is androgen-dependent and has a vital influence on male fertility. Either for therapeutic or preventative purposes, orchidectomy is performed in both human and animal, especially for androgen-dependent disorders. Thereby, the current study aimed to evaluate the immunohistochemical characteristics of the canine prostate gland after unilateral and bilateral orchiectomy. Primarily, twenty-one healthy male dogs of 18–20 kg and 1–2 years were divided into three main groups; control, unilateral castrated and bilateral castrated groups. The last two groups were subdivided, based on post castration time, into three subgroups; 10, 20, and 30 days. The prostatic tissues were harvested from all experimental groups for immunohistochemical studies. The results revealed progressive significant alterations in the prostate gland immunostaining affinity after bilateral castration as time passed; gradual reduction in the androgen receptors and α-SMA immunoexpression was observed. On the contrary, the unilateral procedure conserves the prostatic tissue nearly similar to the control. It was concluded that, however, immunohistochemical characteristics of prostatic tissue were maintained without detectable changes after unilateral castration, there was a marked ongoing decrease of androgen receptors and α-SMA immunoreactivity post bilateral castration procedure.

{"title":"Differential expression of prostatic androgen receptor and alpha-smooth muscle actin in castrated and non-castrated dogs","authors":"Sameh M. Farouk , Amal A.M. Ahmed , Mohamed A. Hashem , Safa H. Badran","doi":"10.1016/j.rvsc.2025.105563","DOIUrl":"10.1016/j.rvsc.2025.105563","url":null,"abstract":"<div><div>The prostate, the only accessory sex gland in dogs, is androgen-dependent and has a vital influence on male fertility. Either for therapeutic or preventative purposes, orchidectomy is performed in both human and animal, especially for androgen-dependent disorders. Thereby, the current study aimed to evaluate the immunohistochemical characteristics of the canine prostate gland after unilateral and bilateral orchiectomy. Primarily, twenty-one healthy male dogs of 18–20 kg and 1–2 years were divided into three main groups; control, unilateral castrated and bilateral castrated groups. The last two groups were subdivided, based on post castration time, into three subgroups; 10, 20, and 30 days. The prostatic tissues were harvested from all experimental groups for immunohistochemical studies. The results revealed progressive significant alterations in the prostate gland immunostaining affinity after bilateral castration as time passed; gradual reduction in the androgen receptors and α-SMA immunoexpression was observed. On the contrary, the unilateral procedure conserves the prostatic tissue nearly similar to the control. It was concluded that, however, immunohistochemical characteristics of prostatic tissue were maintained without detectable changes after unilateral castration, there was a marked ongoing decrease of androgen receptors and α-SMA immunoreactivity post bilateral castration procedure.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"185 ","pages":"Article 105563"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143146623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring endosymbionts and pathogens in Rhipicephalus sanguineus and Ctenocephalides felis felis with Oxford Nanopore Technology

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-02-01 DOI: 10.1016/j.rvsc.2025.105562

Luisa Páez-Triana , David Martinez , Luz H. Patiño , Marina Muñoz , Claudia M. Sandoval-Ramírez , Juan Carlos Pinilla León , Juan David Ramirez

Fleas and ticks play a crucial role in public health as vectors of multiple diseases affecting humans and animals. Several rickettsial pathogens and endosymbionts are transmitted by fleas and ticks. Therefore, understanding this group of microorganisms is essential for fully grasping the spectrum of pathogens transmitted by vectors and the interactions between endosymbiotic microorganisms and their hosts. This study evaluated the presence and diversity of Rickettsiales species in fleas and ticks collected from the Santander department in Colombia. For the methodology a 16S gene amplification approach through Oxford Nanopore sequencing technologies in Rhipicephalus sanguineus and Ctenocephalides felis felis was used. Our findings revealed the presence of multiple pathogenic and endosymbiotic microorganisms, particularly from the Rickettsia and Wolbachia groups. We observed a clear association between Rickettsia species and ticks, while Wolbachia was predominantly found in fleas. Additionally, other important microorganisms were identified, including Anaplasma phagocytophilum, Rickettsia conorii, and different strains of Wolbachia that serve as endosymbionts in various arthropods. These results underscore the importance of fleas and ticks in the transmission of both pathogenic and endosymbiotic microorganisms. The distinct patterns of association between specific pathogens and vectors provide insight into their transmission dynamics. Identifying pathogens such as Anaplasma phagocytophilum and Rickettsia conorii further highlights the need for continued research into vector-borne diseases in Colombia. Understanding the interactions between endosymbionts and pathogenic microorganisms in these vectors could lead to the development of more effective strategies for controlling diseases transmitted by fleas and ticks.

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引用次数: 0

Carbapenem-resistant Enterobacteriaceae from dairy cattle milk: A systematic review and meta-analysis 奶牛牛奶中碳青霉烯耐药肠杆菌科：系统综述和荟萃分析。

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-02-01 DOI: 10.1016/j.rvsc.2024.105497

Dian Meididewi Nuraini , Morsid Andityas , Peerapol Sukon , Patchara Phuektes

Carbapenem-resistant Enterobacteriaceae (CRE) have been detected in dairy cattle milk, raising concerns about public health risks. This study aimed to assess the global prevalence of CRE in dairy cattle milk through a systematic review and meta-analysis, following the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines. Retrieved articles from four databases were initially screened based on predefined inclusion criteria. The meta-analysis included 49 studies (2011–2024), covering 28,134 milk samples and 3462 Enterobacteriaceae isolates globally. Data from the full text were extracted to a Microsoft Excel spreadsheet and analysed using the ‘meta’ R package in R v.4.3.0 software for pooled prevalence and subgroup meta-analysis with a random-effects for logit transformation. Heterogeneity was assessed using Cochran's Q statistic (χ²), p-value and I² statistic. Publication bias and sensitivity were evaluated using Egger's test, funnel plot, trim and fill plot, and leave-one-out test. Globally, the prevalence of CRE in dairy cattle milk was 0.73 % (95 % CI, 0.37–1.41). Subgroup meta-analysis based on continent, sample type, Enterobacteriaceae species, diagnostic method, antibiotic type, and interpretation guideline revealed no significant differences among the criteria within the subgroup. Although the overall pooled prevalence of CRE in dairy cattle milk is relatively low, it raises public health concern regarding raw milk consumption. This emphasizes the need for regular monitoring with in a One Health framework for CRE in the dairy industry to anticipate potential transmission between humans, animals, and the environment.

在奶牛的牛奶中发现了碳青霉烯抗性肠杆菌科（CRE），引起了人们对公共卫生风险的担忧。本研究旨在通过系统评价和荟萃分析，根据系统评价和荟萃分析（PRISMA）指南的首选报告项目，评估奶牛牛奶中CRE的全球流行程度。最初根据预定义的纳入标准筛选从四个数据库检索到的文章。荟萃分析包括49项研究（2011-2024年），涵盖全球28,134份牛奶样本和3462株肠杆菌科分离物。将全文数据提取到Microsoft Excel电子表格中，并使用R v.4.3.0软件中的“meta”R包进行汇总流行率和亚组荟萃分析，并使用随机效应进行logit转换。采用Cochran’s Q统计量（χ2）、p值和I2统计量评估异质性。采用Egger检验、漏斗图、修剪填充图和留一检验评价发表偏倚和敏感性。在全球范围内，奶牛牛奶中CRE的患病率为0.73% （95% CI, 0.37-1.41）。基于大陆、样本类型、肠杆菌科种类、诊断方法、抗生素类型和解释指南的亚群荟萃分析显示，亚群内各标准间无显著差异。虽然奶牛牛奶中CRE的总体流行率相对较低，但它引起了对原料奶消费的公共卫生关注。这强调需要在“同一个健康”框架下对乳制品行业的CRE进行定期监测，以预测在人、动物和环境之间的潜在传播。

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