Research in veterinary science最新文献_第4页

Active transmission of Crimean-Congo Hemorrhagic fever virus, a tick-borne disease, in Iraq: Animal host population movement dynamics and retrospective case study analysis (2018–2023) 蜱传疾病克里米亚-刚果出血热病毒在伊拉克的活跃传播：动物宿主种群流动动态和回顾性病例研究分析（2018-2023）

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-12-17 DOI: 10.1016/j.rvsc.2025.106021

Khalis Ahmed Hammad Ameen , Ameen Saleh Alyousuf , Blesa Abdulhameed Salh , Hiro Mohammed Obaid , Ihab Raqeeb Aakef

This study is a retrospective and descriptive cross-sectional analysis examining demographic and epidemiological data of Crimean-Congo Hemorrhagic Fever Virus (CCHFV) in Iraq, covering confirmed cases from January 2018 to December 2023. Utilizing data from the Iraq CDC/zoonotic department, 1001 cases were analyzed and confirmed via Real-time PCR and ELISA tests at the Central Public Health Laboratory. The study highlights the climatic conditions of Iraq, with its semi-arid and semi-tropical climate influencing disease prevalence. Results indicate a significant gender disparity in CCHFV cases, with 59.4 % male and 40.6 % female patients, suggesting differing exposure and susceptibility between genders. The age distribution shows higher infection rates among individuals aged 22 to 51, potentially linked to occupational exposure and outdoor activities. A notable increase in reported cases was observed in 2022 and 2023, accounting for 38.0 % and 58.6 % of total cases, respectively. This surge may be attributed to environmental and socio-economic factors, including climate change, urbanization, and population displacement.

Geographic analysis reveals that provinces like Thiqar, Baghdad, Basra, and Misan report higher case numbers, indicating regional hotspots for CCHFV transmission. Risk factor analysis emphasizes the role of direct contact with raw meat, animal slaughtering, and tick bites as significant contributors to infection. The study also identifies key symptoms associated with poor prognosis, such as bleeding from injection sites, body orifices, and echymosis, which are significantly correlated with higher mortality rates. These findings underscore the need for targeted public health interventions, including enhanced surveillance, vector control, and public education, particularly in high-risk regions. Understanding the epidemiology and risk factors of CCHFV is crucial for developing effective prevention and control strategies to mitigate the impact of this disease in Iraq.

本研究是一项回顾性和描述性横断面分析，研究了伊拉克克里米亚-刚果出血热病毒（CCHFV）的人口统计学和流行病学数据，涵盖2018年1月至2023年12月的确诊病例。利用伊拉克疾病预防控制中心/人畜共患病部门的数据，在中央公共卫生实验室通过实时聚合酶链反应和酶联免疫吸附试验分析和确认了1001例病例。该研究强调了伊拉克的气候条件，其半干旱和半热带气候影响了疾病的流行。结果显示，CCHFV病例的性别差异显著，男性占59.4%，女性占40.6%，表明性别之间的暴露和易感性存在差异。年龄分布显示，22至51岁人群的感染率较高，这可能与职业接触和户外活动有关。2022年和2023年报告病例数显著增加，分别占总病例数的38.0%和58.6%。这种激增可归因于环境和社会经济因素，包括气候变化、城市化和人口流离失所。地理分析显示，蒂卡尔、巴格达、巴士拉和米桑等省报告的病例数较高，表明了CCHFV传播的区域热点。风险因素分析强调直接接触生肉、动物屠宰和蜱叮咬是导致感染的重要因素。该研究还确定了与预后不良相关的主要症状，如注射部位出血、体腔出血和瘀肿，这些症状与较高的死亡率显著相关。这些发现强调需要有针对性的公共卫生干预措施，包括加强监测、病媒控制和公共教育，特别是在高风险地区。了解CCHFV的流行病学和危险因素对于制定有效的预防和控制战略以减轻该疾病在伊拉克的影响至关重要。

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引用次数: 0

Epidemiology of endoparasite infections in the European hedgehog (Erinaceus europaeus) and association with rehabilitation outcome in wildlife centres in Northwest France 法国西北部野生动物中心欧洲刺猬（Erinaceus europaeus）体内寄生虫感染的流行病学及其与康复结果的关系

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-12-17 DOI: 10.1016/j.rvsc.2025.106034

Paula Yagüez I. López-Jurado , Katie Beckmann , Rob Kelly

The European hedgehog (Erinaceus europaeus) is prevalent across Western Europe, with many individuals admitted to wildlife rehabilitation centres. Parasitic infections, particularly helminth parasites, may threaten rehabilitation success. This study investigated respiratory and gastrointestinal endoparasite prevalence, risk factors, and associations with survival in 300 hedgehogs admitted to four rehabilitation centres in northwestern France (May 2023–May 2024). Faecal samples were analysed using either the modified McMaster technique (Oise) or direct smear (Seine-Maritime, Loir-et-Cher, Essonne). Overall, 58.3 % of hedgehogs carried at least one endoparasite. The most common were Capillaria species group (43.6 %) and Crenosoma striatum (25.7 %), followed by Brachylaemus erinacei (11 %) and Cycloisospora species group (7.3 %). Co-infection was common; 35 % of infected individuals harboured both Capillaria spp. and C. striatum. Younger hedgehogs showed lower prevalence but higher parasite burdens, suggesting reduced resistance. Capillaria spp. prevalence peaked in winter across all age groups. Multivariable analysis identified age, weight, season, and admission reason as significant risk factors for parasitism. Importantly, detection of C. striatum significantly increased mortality risk during rehabilitation (OR = 3.44, p = 0.0002). These findings highlight the need for targeted parasite screening and treatment protocols for specific risk groups, for example for juveniles and individuals with clinical signs. Coprological analysis offers a practical, non-invasive tool for parasite detection in resource-limited rehabilitation settings. This is the first study to report endoparasite prevalence in live hedgehogs in France using coprological methods and to demonstrate a statistical association between C. striatum infection and mortality in rehabilitation.

欧洲刺猬（Erinaceus europaeus）在西欧普遍存在，许多个体被送入野生动物康复中心。寄生虫感染，特别是蠕虫寄生虫，可能威胁康复的成功。本研究调查了法国西北部4个康复中心（2023年5月至2024年5月）入院的300只刺猬的呼吸道和胃肠道内寄生虫的流行情况、危险因素及其与生存率的关系。使用改良的麦克马斯特技术（Oise）或直接涂片（Seine-Maritime, Loir-et-Cher, Essonne）对粪便样本进行分析。总体而言，58.3%的刺猬携带至少一种内寄生虫。最常见的是毛缕虫（43.6%）和纹状毛蕊虫（25.7%），其次是毛缕虫（11%）和环异孢子虫（7.3%）。合并感染较为常见；35%的受感染个体同时携带毛线虫和纹状体线虫。年轻的刺猬患病率较低，但寄生虫负担较高，表明抗性降低。在所有年龄组中，毛细线虫的患病率在冬季达到高峰。多变量分析发现年龄、体重、季节和入院原因是寄生虫病的重要危险因素。重要的是，纹状体的检测显著增加康复期间的死亡风险（OR = 3.44, p = 0.0002）。这些发现强调需要针对特定风险群体，例如青少年和有临床症状的个体，制定有针对性的寄生虫筛查和治疗方案。在资源有限的康复环境中，coprology分析为寄生虫检测提供了一种实用的、非侵入性的工具。这是第一个使用粪学方法报道法国活刺猬体内寄生虫流行情况的研究，并证明纹状体感染与康复死亡率之间存在统计学关联。

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引用次数: 0

Zoledronate-induced activation of γδ T cells is associated with NK cell activation and reduced parasite burden in the cecum of Eimeria tenella-infected chicks 唑来膦酸钠诱导的γδ T细胞活化与幼美耳球虫感染雏鸡盲肠内NK细胞活化和寄生虫负荷减少有关。

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-12-17 DOI: 10.1016/j.rvsc.2025.106030

Quan Viet Le , Makoto Matsubayashi , Toshimitsu Hatabu

This study aimed to elucidate the role of γδ T cells in chicks infected with Eimeria tenella. Zoledronate was administered in vivo to activate γδ T cells. White Leghorn chicks were divided into four groups: zoledronate-treated (ZOL), infected control (CT), uninfected zoledronate-treated (UN/ZOL), and uninfected control (UN). Chicks in the CT and ZOL groups were orally inoculated with sporulated E. tenella oocysts (1 × 10^4 oocysts/chick) at 14 days of age. Fecal samples were collected between 4 and 15 days post-infection (dpi) to assess oocyst shedding. Cecal samples were obtained at 5 dpi for histopathological examination and gene expression analysis. Lesion and parasite burden scores were significantly reduced in the ZOL group compared with the CT group. Expression levels of IL-17 A, IL-21, and IFN-γ were significantly higher in the ZOL group, whereas IL-13 and PGES expression was significantly higher in the CT group. These findings indicate that activation of γδ T cells alleviates cecal tissue damage caused by E. tenella infection. Moreover, they suggest that NK cells activated by IL-21 may play a role in parasite control and contribute to the establishment of the local immune environment, but the effect of parasite control by IL-21-induced NK cell activation requires further research. Future studies should also explore whether γδ T cell activation and IL-21-mediated immunity are associated with mechanisms of parasite control.

本研究旨在阐明γδ T细胞在雏鸡感染柔嫩艾美耳球虫中的作用。体内给药唑来膦酸钠活化γδ T细胞。将来港白雏鸡分为唑来膦酸盐处理组（ZOL）、感染对照组（CT）、未感染唑来膦酸盐处理组（UN/ZOL）和未感染对照组（UN） 4组。CT组和ZOL组雏鸡在14日龄时口服带孢子的柔嫩E.卵囊（1 × 10^4个/只）。在感染后4至15天收集粪便样本以评估卵囊脱落情况。5 dpi时取盲肠标本进行组织病理学检查和基因表达分析。与CT组相比，ZOL组的病变和寄生虫负担评分明显降低。ZOL组IL-17 A、IL-21、IFN-γ表达水平显著高于对照组，而CT组IL-13、PGES表达水平显著高于对照组。这些结果表明，γδ T细胞的活化可减轻tenella感染引起的盲肠组织损伤。此外，他们认为IL-21活化的NK细胞可能在寄生虫控制中发挥作用，有助于建立局部免疫环境，但IL-21诱导的NK细胞活化对寄生虫的控制效果还需要进一步研究。未来的研究还应探讨γδ T细胞活化和il -21介导的免疫是否与寄生虫控制机制有关。

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引用次数: 0

Pilot study: Effects of whole-body vibrational therapy on equine thoracolumbar epaxial muscle response to pressure algometry 初步研究：全身振动疗法对马胸腰椎外轴肌对压力测量反应的影响。

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-12-15 DOI: 10.1016/j.rvsc.2025.106027

Riley Leibeck , Jeffrey Lehman , Sheri Birmingham

Background

Whole-body vibrational therapy (WBV) is well-studied in people, but there is a paucity of research regarding its effect on horses. It is suggested that the use of WBV in horses increases performance, aids in recovery, and stimulates nerves, bones, and muscles in ways that normal exercise does not.

Objective

The purpose of this pilot study was to determine if frequent WBV therapy sessions affect mechanical nociceptive thresholds based on pressure algometry in horses without clinical signs of lameness or back pain.

Methods

The mechanical nociceptive thresholds (MNTs) were measured for 5 horses at 8 points along their thoracolumbar epaxial muscles once every 7 days for 8 weeks. Baseline MNT scores were established during a control period with no vibration therapy treatment over the first 4 weeks. During the second 4-week period, horses received 5, 30-min vibration therapy sessions per week.

Results

Despite previous research indicating that WBV increases epaxial muscle symmetry in horses, no significant difference was noted between the horses' mean MNTs across all 8 sites during the baseline and treatment periods. Upon further investigation of the change in sensitivity at the individual sites across all subjects, a statistically significant (p < 0.05) increase in MNT (0.49–0.62 kgf) was observed at 4 out of the 8 sites.

Conclusion

While an increase in mean MNTs was not observed, this study found that WBV may increase MNTs at individual sites along the thoracolumbar epaxial musculature in horses. Further investigation is required to assess whether this difference is biologically meaningful and repeatable.

背景：全身振动疗法（WBV）在人身上得到了很好的研究，但关于其对马的影响的研究却很少。这表明，在马身上使用WBV可以提高表现，帮助恢复，并刺激神经，骨骼和肌肉，这是正常运动所不能达到的。目的：本初步研究的目的是确定频繁的WBV治疗是否会影响无跛行或背痛临床症状的马的机械伤害感受阈值。方法：测定5匹马胸腰段外轴肌8个点的机械伤害知觉阈值（mnt），每7天1次，连续8周。基线MNT评分是在前4周没有振动治疗的对照期内建立的。在第二个4周期间，马每周接受5次30分钟的振动治疗。结果：尽管先前的研究表明，WBV增加了马的外轴肌对称性，但在基线和治疗期间，马在所有8个部位的平均mnt之间没有显着差异。结论：虽然没有观察到平均MNTs的增加，但本研究发现，WBV可能会增加马胸腰椎外轴肌组织个别部位的MNTs。需要进一步的调查来评估这种差异是否具有生物学意义和可重复性。

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引用次数: 0

Comparative evaluation of otological examination techniques in captive Leopardus guttulus 圈养喉豹耳科检查技术的比较评价。

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-12-15 DOI: 10.1016/j.rvsc.2025.106028

Maria C.A. Martini , Ana L.R. Marques , Marina Marangoni , Gabrielle C. Freitas , Gentil F. Golçalves , Zalmir S. Cubas , Wanderlei de Moraes , Fabíola Dalmolin , Tatiana Champion

This study evaluated the use of conventional otoscopy, digital otoscopy, and video-otoscopy for the examination of the external ear canal and tympanic membrane in 14 southern tiger cats (Leopardus guttulus). While traditional otoscopic methods did not allow detailed visualization of the epithelium or cerumen, the video-otoscopy technique provided superior image magnification, enabling better identification of anatomical structures, including vascularization of the tympanic membrane. However, mild bleeding was observed in two individuals, likely due to overheating of the xenon light source. Anatomically, the auricle, external acoustic meatus, and tympanic membrane of L. guttulus were similar to those of domestic cats, with a cartilaginous prominence hindered access to the horizontal ear canal in some cases. Cytological analysis revealed desquamated epithelial cells in all individuals, and Malassezia spp. was identified in one animal, highlighting the relevance of fungal monitoring in captive wildlife. Bacterial cultures revealed the presence of coagulase-negative Gram-positive cocci suggestive of Staphylococcus spp. in three samples, with minimal antibiotic resistance. Two older animals presented small, benign-appearing masses near the tympanic membrane. Overall, video-otoscopy was the most effective method for otological examination, offering high-definition imaging and documentation capabilities, although its use requires caution to prevent thermal injury.

本研究对14只南方虎（Leopardus guttulus）的外耳道和鼓膜进行了常规耳镜、数字耳镜和视频耳镜检查。虽然传统的耳镜方法不能详细观察上皮或耳膜，但视频耳镜技术提供了优越的图像放大，能够更好地识别解剖结构，包括鼓膜的血管化。然而，在两个人中观察到轻微出血，可能是由于氙气光源过热。在解剖学上，耳猫的耳廓、外耳道和鼓膜与家猫相似，在某些情况下，软骨突出阻碍了进入水平耳道。细胞学分析显示所有个体的上皮细胞脱落，并在一只动物中鉴定出马拉色菌，突出了圈养野生动物真菌监测的相关性。细菌培养显示存在凝固酶阴性革兰氏阳性球菌提示葡萄球菌的三个样本，与最小的抗生素耐药性。两只老年动物在鼓膜附近出现小而良性的肿块。总的来说，视频耳镜检查是耳科检查最有效的方法，它提供了高清成像和记录功能，尽管它的使用需要注意防止热损伤。

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引用次数: 0

Femorotibial joint rotation in intact joints and following partial medial collateral ligament transection, tibial plateau leveling osteotomy, and cranial cruciate ligament transection – A limb press study 完整关节及部分内侧副韧带横断、胫骨平台平截骨和颅交叉韧带横断后的股胫关节旋转-肢体按压研究

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-12-15 DOI: 10.1016/j.rvsc.2025.106026

M.L. Holmbjerg , C.K. Tvedsborg , M.B.M. Nielsen, J.E. Miles

Despite widespread use of tibial plateau leveling osteotomy (TPLO), the influence of partial medial collateral ligament transection (pMCLX) and physiologic loading on rotational stability remains unclear. This cadaveric study quantified internal and external tibial rotation in canine stifles under simulated weightbearing conditions. Ten unpaired pelvic limbs were mounted in a custom limb press applying axial loads equivalent to 30 % body weight with the stifle fixed at 135°. Quadriceps tension was simulated using a load cell and turnbuckle, and controlled internal and external torques (0.007 Nm/kg) were applied twice per condition. Joints were tested sequentially: intact, after pMCLX at the level of the planned TPLO osteotomy, following TPLO (target tibial plateau angle 5°), and after cranial cruciate ligament transection (CCLX). Internal rotation changed minimally after pMCLX (P = 1.0) but increased significantly following TPLO (P = 0.001) and CCLX (P = 0.002), with limited changes following CCLX (P = 1.0). External rotation increased significantly only between TPLO and CCLX (P = 0.02). These findings indicate that pMCLX does not contribute to significant rotational laxity, while TPLO increases internal rotation independently of CCL integrity. The stabilizing effects of quadriceps tension and axial loading highlight the importance of physiologic constructs in ex vivo models. Overall, these results challenge concerns about iatrogenic instability from pMCLX and support current TPLO positioning strategies.

尽管广泛使用胫骨平台水平截骨术（TPLO），但部分内侧副韧带横断（pMCLX）和生理负荷对旋转稳定性的影响尚不清楚。该尸体研究量化了模拟负重条件下犬科动物的胫骨内外旋转。将10个未配对的骨盆肢体安装在定制的肢体压力机中，施加相当于30%体重的轴向载荷，将膝关节固定在135°。使用称重传感器和turnbuckle模拟股四头肌张力，并在每种情况下施加两次可控内外扭矩（0.007 Nm/kg）。依次对关节进行测试：完整，在计划TPLO截骨水平进行pMCLX后，TPLO（目标胫骨平台角5°）后，以及颅十字韧带横断（CCLX）后。内部旋转在pMCLX后变化最小（P = 1.0），但在TPLO （P = 0.001）和CCLX （P = 0.002）后显著增加，CCLX后变化有限（P = 1.0）。外旋仅在TPLO和CCLX组显著增加（P = 0.02）。这些研究结果表明，pMCLX不会导致显著的旋转松弛，而TPLO会独立于CCL完整性增加内部旋转。股四头肌张力和轴向负荷的稳定作用突出了生理构造在离体模型中的重要性。总的来说，这些结果挑战了人们对pMCLX医源性不稳定性的担忧，并支持了当前的TPLO定位策略。

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引用次数: 0

Spatiotemporal distribution and genetic characteristics of porcine reproductive and respiratory syndrome virus-2 lineage 1 in Japan from 2020 to 2023 2020 - 2023年日本猪繁殖与呼吸综合征病毒2谱系1的时空分布及遗传特征

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-12-13 DOI: 10.1016/j.rvsc.2025.106025

Ryosuke Takai , Tetsuo Sato , Yoriko Yonezawa , Nobuyuki Tsutsumi , Ryohei Nukui , Katsuaki Sugiura , Osamu Taira

Porcine reproductive and respiratory syndrome (PRRS), caused by the PRRS virus (PRRSV), is a major swine disease globally. This study analyzed 1207 PRRSV sequences (2020-2023) detected through routine diagnostic submissions in Japan using the standardized open reading frame 5-based lineage classification system. Phylogenetic analysis identified six lineages in Japan: L1, L4, L5, L8, L9, and L11. Notably, the prevalence of L1 showed a marked increase during the study period. L1 strains were predominantly detected in the Kanto and Kyushu regions. In Kanto, L1B was predominant (80 %), whereas Kyushu exhibited greater sublineage diversity (L1A, L1B, L1E, and L1I). Pairwise nucleotide identity analysis revealed high genetic heterogeneity within the L1 population (mean identity 90.1 %), while L5 and L8 populations were highly homogenous (97.9 % and 98.3 %, respectively) and showed high sequence identity (>95 %) to commercial vaccine strains. This study presents the first nationwide molecular epidemiological analysis of PRRSV in Japan based on the global lineage system. The findings highlight Japan's distinct L1 sublineage dynamics (e.g., L1B dominance) compared to global trends (e.g., L1C.5 in the US and NADC30/34-like strains in China and Korea), offering critical insights for informing effective PRRSV control strategies.

猪繁殖与呼吸综合征（PRRS）是由猪繁殖与呼吸综合征病毒（PRRSV）引起的一种全球性的主要猪疾病。本研究使用基于标准化开放阅读框5的谱系分类系统，分析了通过日本常规诊断提交检测到的1207个PRRSV序列（2020-2023）。系统发育分析在日本鉴定出6个谱系：L1、L4、L5、L8、L9和L11。值得注意的是，在研究期间，L1的患病率明显增加。L1菌株主要在关东和九州地区检出。关东地区以L1B亚系为主（80%），九州地区则表现出较大的亚系多样性（L1A、L1B、L1E和L1I）。两两核苷酸同源性分析显示，L1群体具有较高的遗传异质性（平均同源性为90.1%），而L5和L8群体具有较高的同源性（分别为97.9%和98.3%），与商业疫苗株具有较高的序列同源性（> 95%）。本研究首次基于全球谱系系统对日本PRRSV进行了全国范围的分子流行病学分析。与全球趋势（如L1C）相比，研究结果突出了日本独特的L1亚谱系动态（如L1B主导）。5在美国，nadc30 /34样毒株在中国和韩国)，为通报有效的PRRSV控制策略提供了关键见解。

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引用次数: 0

Evaluating attitudes toward piglet euthanasia among Spanish-speaking animal caretakers on U.S. commercial swine farms: A comparison of CO₂ and non-penetrating captive bolt methods 评估美国商业养猪场中讲西班牙语的动物饲养员对小猪安乐死的态度：CO₂和非穿透式捆绑螺栓方法的比较

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-12-11 DOI: 10.1016/j.rvsc.2025.106023

Brooke Kitting , Faby Hernandez , Pablo Lamino , John J. McGlone , Heidi Villalba , Allen Jimena Martinez Aguiriano , Miguel Alejandro Hernandez Corrales , Daniela Sofia Lima Alvarez , Arlene Garcia

The use of a non-penetrating captive bolt (NPCB) gun to euthanize suckling pigs may be visually challenging and stressful to animal caretakers, influencing their ability to make timely euthanasia decisions. The objective of this study was to utilize a survey to characterize the emotional and practical challenges of Spanish-speaking animal caretakers (n = 257) employed on commercial swine farms in the United States, as they transitioned from carbon dioxide (CO₂) gas euthanasia to NPCB as the primary method of euthanasia in suckling piglets. The survey results indicated concerns primarily centered on perceived inhumaneness and potential pain inflicted on piglets, particularly with the NPCB method. Caretakers expressed concerns about the psychological impact of CO₂, but felt it was a more humane alternative than NPCB. Male caretakers were less likely than females to dislike euthanizing piglets (p = 0.013). Animal caretakers who were aware of the Common Swine Industry Audit (CSIA; p = 0.010), animal caretakers with a college degree (p < 0.05), and those who decided when to euthanize an animal and conducted the procedure themselves preferred to have someone else perform the task of euthanasia (p < 0.01). These findings highlight how demographics and knowledge of the CSIA influence animal caretakers' decisions and preferences regarding piglet euthanasia. These findings also emphasize a challenge with animal caretakers' reluctance to accept science-based animal welfare practices.

使用非穿透式螺栓（NPCB）枪对乳猪实施安乐死可能会给动物饲养员带来视觉上的挑战和压力，影响他们及时做出安乐死决定的能力。本研究的目的是利用一项调查来描述在美国商业养猪场雇用的讲西班牙语的动物饲养员（n = 257）的情感和实际挑战，因为他们从二氧化碳（CO 2）气体安乐死过渡到NPCB作为哺乳仔猪安乐死的主要方法。调查结果表明，人们的担忧主要集中在仔猪感受到的不人道和潜在的疼痛上，特别是使用NPCB方法时。管理人员对二氧化碳的心理影响表示担忧，但认为这是一种比NPCB更人道的选择。男性看护人不喜欢对仔猪实施安乐死的可能性低于女性（p = 0.013）。了解普通养猪业审计（CSIA; p = 0.010）的动物饲养员，拥有大学学位的动物饲养员（p < 0.05），以及那些决定何时对动物实施安乐死并自己实施安乐死的人更喜欢让别人执行安乐死任务（p < 0.01）。这些发现强调了人口统计学和对CSIA的了解如何影响动物饲养员对小猪安乐死的决定和偏好。这些发现还强调了动物饲养者不愿接受基于科学的动物福利实践的挑战。

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引用次数: 0

Bordetella hinzii isolated from samples of commercially available raw pet food for cats in the United Kingdom (SHORT COMMUNICATION) 从英国市售的猫用生宠物食品中分离出的欣茨氏杆菌（简短通讯）

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-12-11 DOI: 10.1016/j.rvsc.2025.106024

Genever Morgan , Isabelle Kingsley , Jenny Llewelyn , Sam Haldenby , Nicola Williams , Dorina Timofte

Raw meat diets for pets are frequently contaminated with potentially pathogenic and zoonotic bacteria. Here we present the first report, to our knowledge, of Bordetella hinzii, a respiratory pathogen of poultry and rare but emerging opportunistic respiratory pathogen in humans, detected incidentally in commercially available frozen pre-prepared raw meat diets for cats. B. hinzii was isolated from five samples of raw meat diets, from two separate brands, purchased from a retailer in the United Kingdom. Samples were a single-protein source and comprised chicken, beef, and rabbit. Whole genome sequencing indicated the presence of antimicrobial resistance genes with potential to confer resistance to multiple drug classes, including classes of highest priority critically important antibiotics. Whilst this study was limited by a small dataset and additional research surrounding the risks of transmission of B. hinzii is required, this novel finding indicates a further potential risk associated with the provision of raw meat diets for pets, particularly for the immunocompromised, that warrants further investigation.

宠物的生肉饮食经常被潜在的致病菌和人畜共患病细菌污染。在这里，据我们所知，我们提出了关于hinzii博德泰拉的第一份报告，这是一种家禽呼吸道病原体，也是一种罕见但正在出现的人类机会性呼吸道病原体，偶然在市售的冷冻预准备生肉猫粮中发现。从从英国零售商处购买的来自两个不同品牌的5份生肉日粮样品中分离出B. hinzii。样品是单一蛋白质来源，包括鸡肉、牛肉和兔肉。全基因组测序表明，存在抗微生物药物耐药基因，可能对多种药物产生耐药性，包括最优先的至关重要的抗生素。虽然这项研究的数据量很小，还需要对hinzi病菌的传播风险进行更多的研究，但这一新发现表明，为宠物提供生肉饮食存在进一步的潜在风险，特别是对免疫功能低下的宠物，值得进一步调查。

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引用次数: 0

Equine oviduct-specific glycoprotein is modulated by hormones and sperm cells 马的输卵管特异性糖蛋白受激素和精子细胞的调节。

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-12-11 DOI: 10.1016/j.rvsc.2025.106022

Pedro Pinto-Bravo , Maria Rosa Rebordão , Ana S. Amaral , Anna Szóstek-Mioduchowska , Carina Fernandes , António M. Galvão , Elisabete Silva , Joana Alpoim-Moreira , Rosário P. Roberto da Costa , Dariusz J. Skarzynski , Graça M Ferreira-Dias

Oviduct glycoprotein 1 (OVGP1) is a key protein involved in oviductal functions. β-estradiol (E₂) and progesterone (P₄), oxytocin (OXT) and tumor necrosis factor-α (TNFα) modulate the equine oviduct function, through prostaglandin regulation. The objective was to evaluate OVGP1 expression within each equine oviduct segment (infundibulum, ampulla isthmus), throughout the estrous cycle. The in vitro effect of (i) E₂, P₄, OXT, TNFα; and (ii) spermatozoa, on oviduct OVGP1 transcription and secretion was studied. Gene transcription was assessed by real-time PCR; protein expression by western blot; and protein production by enzyme immunoassay. OVGP1 mRNA increased in the ampulla, in the early-luteal phase (P < 0.05). OVGP1 protein expression increased in the follicular phase, in all portions (P < 0.05). A temporal desynchronization between transcription and protein synthesis might maintain oviduct function. In ampulla explants, OXT and TNFα up-regulated OVGP1 transcripts in follicular phase; E₂ in early-luteal phase; and P₄ in mid-luteal phase (P < 0.05). OXT and TNFα effect on OVGP1 transcripts might be ascribed to prostaglandin modulation. Oviductal endogenous E₂ in follicular phase, could prime E₂ stimulation of OVGP1 transcripts in early-luteal phase. The stimulatory effect of P₄ on OVGP1 transcripts may modulate early embryogenesis. OVGP1 in vitro production was not dependent of E₂, P₄, OXT or TNFα treatments. Sperm cells, either in direct or indirect contact with oviduct explants, up-regulated OVGP1 production, in the isthmus (P < 0.05). These data suggest that OVGP1 modulates sperm and mare's oviduct cross-talk and may play an important role in improving assisted reproductive technologies.

输卵管糖蛋白1 （OVGP1）是参与输卵管功能的关键蛋白。β-雌二醇（E2）、孕酮（P4）、催产素（OXT）和肿瘤坏死因子-α (tnf -α)通过前列腺素调节马的输卵管功能。目的是评估在整个发情周期中，OVGP1在马各输卵管段（输卵管、壶腹峡）内的表达。(1) E2、P4、OXT、TNFα的体外作用；（ii）精子对输卵管OVGP1转录和分泌的影响。实时荧光定量PCR检测基因转录；western blot蛋白表达；以及通过酶免疫分析法产生的蛋白质。OVGP1 mRNA在壶腹、黄体早期升高(p_2在黄体早期升高，P4在黄体中期升高（p_2在卵泡期升高），可以启动E2刺激黄体早期OVGP1转录本。P4对OVGP1转录本的刺激作用可能调节早期胚胎发生。OVGP1的体外生成不依赖于E2、P4、OXT或TNFα处理。在峡部，与输卵管外植体直接或间接接触的精子细胞上调了OVGP1的产生(P

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引用次数: 0