Research in veterinary science最新文献_第5页

Understanding the bovine mastitis co-infections: Coexistence with Enterobacter alters S. aureus antibiotic susceptibility and virulence phenotype

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-01-21 DOI: 10.1016/j.rvsc.2025.105547

Paula Isaac , María Laura Breser , María Florencia De Lillo , Luciana Paola Bohl , Luis Fernando Calvinho , Carina Porporatto

The World Health Organization recently reported an alarming evolution and spread of antibiotic resistance, a global risk factor recognized as a One Health challenge. In veterinary, the general lack of clear treatment guidelines often leads to antibiotic misuse. Bovine mastitis is responsible for major economic losses and the main cause of antibiotic administration in the dairy industry, favoring the emergence of multi-resistant phenotypes. The complexity of inter-microbial and host-pathogen interactions in the mammary gland, demonstrated by culture-independent techniques, not only complicates the prediction of antibiotic treatment outcomes but also underscores the urgent need for further research in this field. This work evaluated the interactions between S. aureus L33 and Enterobacter sp. L34 obtained from an intramammary co-infection. The behavior of the dual-species culture resembled that of the Enterobacter monoculture in all the evaluated contexts. Most of the selected S. aureus virulence factors and the antibiotic susceptibility were altered by coexisting with Enterobacter. Under the protection of Enterobacter, S. aureus was able to survive upon exposure to concentrations of cloxacillin and other antibiotics that would be bactericidal for the monoculture. This could have serious implications for bacterial clearance of mastitis originating from the underestimated co-infections. These findings highlight the importance of broadening our knowledge of how microbial interactions in intramammary infections could contribute to antibiotic treatments failures. Moreover, they open new perspectives for the design of bovine mastitis therapies that consider the ecological context in order to optimize the antibiotic usage, improve the success rates and reduce antibiotic resistance.

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引用次数: 0

Cartilage defect identification on computed tomography arthrography in equine fetlock: Ex-vivo study

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-01-21 DOI: 10.1016/j.rvsc.2025.105548

Irene Nocera , Chiara Di Franco , Elisa Marcucci , Caterina Puccinelli , Giulia Sala , Micaela Sgorbini , Simonetta Citi

CT arthrography (CTA) for evaluating cartilage defects would be beneficial in clinical practice. Since the contrast medium (CM) volume is often driven by operator experience, the aim was to evaluate the minimum volume of iodinated CM for CTA sufficient to identify iatrogenic cartilage defects in horse fetlock. The study was conducted on ex-vivo 32 distal limbs from adult horses collected at slaughter. Iatrogenic cartilage defects were arthroscopically made on fetlock, at 5 predetermined locations. Subsequently, CT scans were performed, according to the following protocol: precontrast scan, followed by subsequent scans with incremental volumes of CM, ranging from 2,5–40 ml (total of 10 scans per fetlock). Then, the cartilage defects were measured macroscopically using a calliper. CTA scans were evaluated by a single operator, and defects were assessed for presence/absence and volume measures. Data were expressed as median and interquartile values. The association between CT scan assessments and different CM volumes was examined with chi-square test. The correlation between CT scans and macroscopic assessments was evaluated using Spearman's coefficient. Significance was set at a p-value <0.05. Twenty millilitres of CM were required to visualize 100 % of the defects, regardless of the location. A weak correlation was found between the defects' volume on macroscopic and CT scan assessment. Twenty millilitres of CM was sufficient to identify cartilage defects on the fetlock articular surface, supporting the conscious use of CM adequate for specific diagnostic purposes.

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引用次数: 0

Corrigendum to “Biosynthesis and regulation mechanisms of the Pasteurella multocida capsule” [Res Vet Sci. 127 (2019):82–90/ Doi: 10.1016/j.rvsc.2019.10.011] “多杀性巴氏杆菌胶囊的生物合成及调控机制”[j].兽医科学，127 (2019):82-90/ Doi: 10.1016/j.rvsc.2019.10.011。

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-01-21 DOI: 10.1016/j.rvsc.2025.105542

Lijun Guan , Yun Xue , Wenwen Ding , Zhanqin Zhao

引用次数: 0

Veterinarians approach towards antimicrobial stewardship and one health: A survey study

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-01-19 DOI: 10.1016/j.rvsc.2025.105546

Falguni S. Thakar , Kiran Vasava , Snehal Bagtharia , Amit Kanani , Vartika Chandra , Beena Patel , Dipeeka K. Mandaliya

Antimicrobial resistance (AMR) is one of the top 10 global public health threats. One Health approach is needed to tackle antimicrobial resistance as most antimicrobials used in animals are also used in humans and affect the environment. Although the knowledge and attitudes of personnel in the human healthcare sector are studied more frequently in Gujarat, veterinarians' approaches also play a pivotal role in attaining antimicrobial stewardship. Thus, an online survey was conducted to understand the knowledge, attitudes, and practices of veterinarians regarding AMR and antimicrobial stewardship in Gujarat. A total of 691 respondents included 300 government veterinarians (44 %), 258 cooperative veterinarians (37 %), 45 private veterinarians (5 %), 69 MVD GVK veterinarians (10 %), and 19 others (4 %). Several veterinarians (77 %) believe that farmers' misuse of antimicrobials contributes significantly to AMR. According to 78 % of respondents, the inappropriate use of antimicrobials in food-producing animals contributes to AMR, which can be reduced by vaccines, biosecurity measures, and hygiene (79 %). As per 86 % of the responders, antimicrobial stewardship is important, and 81 % educate farmers on the rational use of antimicrobials. Though 92 % of veterinarians agreed that culture and antimicrobial sensitivity testing (AST) should be done as frequently as possible to guide antimicrobial usage, only 4 % always, and 68 % sometimes send samples for AST. However, 31 % think it is acceptable to commence antimicrobial therapy without a confirmatory diagnosis. The study findings suggest that AMR awareness among farmers, increased provision of laboratory services, and AST at affordable costs should be developed to combat the development of AMR.

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引用次数: 0

A longitudinal study on the detection of anti-Leishmania antibodies in a captive European mink (Mustela lutreola) population and their correlation with serum protein electrophoresis 欧洲水貂（Mustela lutreola）群体抗利什曼原虫抗体检测及其与血清蛋白电泳相关性的纵向研究。

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-01-18 DOI: 10.1016/j.rvsc.2025.105541

María del Carmen Aranda , José Villora , Jacobo Giner , Antonio Fernández , Pablo Quilez , Janine Elizabeth Davies , María D. Pérez , Sergio Villanueva-Saz , Diana Marteles

Leishmaniosis, caused by Leishmania infantum and transmitted by sand flies, is a significant zoonotic disease. Dogs are primary reservoirs, but other domestic animals, such as cats and ferrets, and wild species, including Eurasian otters and European mink, can be hosts. This study focused on European mink, a critically endangered species, investigating the seroprevalence of L. infantum antibodies and its correlation with serum protein profiles over 2021–2024. A total of 68 captive minks in Spain were monitored, yielding 105 serum samples. ELISA tests identified 44 seropositive samples (41.9 %), with seroprevalence varying annually. While no significant association was found between seropositivity and age, gender, or body condition, seropositivity correlated significantly with the year of collection. Some animals exhibited fluctuating antibody levels across sampling periods. Serum protein electrophoresis analyzed 96 samples, revealing that L. infantum seropositivity significantly affected total protein concentrations, β-globulins, γ-globulins, and albumin:globulin ratio. Variations were also observed in specific protein fractions among both seropositive and seronegative minks. These findings highlight European mink's potential epidemiological role in L. infantum transmission. Monitoring antibody levels and protein profiles in captive populations can inform conservation strategies and disease management efforts.

利什曼病是一种重要的人畜共患疾病，由幼利什曼原虫引起，经沙蝇传播。狗是主要的宿主，但其他家畜，如猫和雪貂，以及野生物种，包括欧亚水獭和欧洲水貂，也可以是宿主。本研究以欧洲水貂为研究对象，研究了2021-2024年欧洲水貂婴幼儿乳杆菌抗体的血清阳性率及其与血清蛋白谱的相关性。西班牙共监测68只圈养水貂，获得105份血清样本。ELISA检测发现44个血清阳性样本（41.9%），血清阳性率每年变化。虽然血清阳性与年龄、性别或身体状况之间没有显著关联，但血清阳性与采集年份显著相关。一些动物在采样期间表现出波动的抗体水平。血清蛋白电泳分析96份样品，结果显示婴儿乳杆菌血清阳性显著影响总蛋白浓度、β-球蛋白、γ-球蛋白和白蛋白：球蛋白比。血清阳性和血清阴性水貂的特定蛋白组分也存在差异。这些发现强调了欧洲水貂在婴幼儿乳腺炎传播中的潜在流行病学作用。监测圈养种群的抗体水平和蛋白质谱可以为保护策略和疾病管理工作提供信息。

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引用次数: 0

Enniatin B alters bovine polymorphonuclear leukocytes phagocytosis and extracellular reactive oxygen species production in vitro

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-01-16 DOI: 10.1016/j.rvsc.2025.105540

S. Sandrini, G. Salvi, D. Ravanelli , V. Perricone, L.G. De Matos, F. Ceciliani, C. Lecchi, A. Agazzi

Enniatins (ENNs) affect human and animal health. Different ENN analogs have been identified, but Enniatin B (ENN B) is the most detected in foods and feeds. This study investigated the effect of ENN B on bovine polymorphonuclear leukocytes (PMNs) challenged with increasing ENN B concentrations (0.625, 1.25, 2.5, 5, and 10 μM). Bovine PMNs were isolated from the peripheral blood of dairy cows to evaluate the cell viability, chemotactic function, ability to phagocyte Gram+ and Gram– microorganisms, and extracellular Reactive Oxygens Species (ROS), with or without phorbol 12-myristate-13-acetate (PMA) as a pro-inflammatory challenge. Results demonstrated that ENN B did not affect bovine PMN viability and chemotactic activity at all concentrations (p = 0.952; p = 0.218, respectively). E. coli and S. aureus phagocytosis ability were reduced by ENN B at the highest concentrations (5 and 10 μM) compared to the negative control (p ≤ 0.001; p = 0.001, respectively). Extracellular ROS production was increased by ENN B challenge under physiological and pro-inflammatory conditions (p = 0.014; p < 0.001, respectively). In conclusion, ENN B did not exert cytotoxic effects on bovine PMNs, while reduced phagocytic ability and increased the production of extracellular ROS, highlighting its potential role as an immunomodulator of the bovine innate immune response in vitro.

Implications

Emerging mycotoxin Enniatin B is a common grain contaminant worldwide that can exert cytotoxic and immunotoxic effects in animal cells. We hypothesized that Enniatin B could in vitro affect the bovine immune response. In our study, Enniatin B did not affect bovine polymorphonuclear cell viability and chemotaxis, while a reduction of phagocytosis and a modulation of extracellular reactive oxygen species were observed. The present study shows that Enniatin B in vitro exerts a potential role as an immunomodulator of the bovine innate immune response putting animals at an increased risk of infection diseases.

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引用次数: 0

Development of a duplex loop-mediated isothermal amplification together with lateral flow dipstick assay for the detection and discrimination of parasitic infections in chickens between cestodes belonging to genus Raillietina and trematodes in family Echinostomatidae 采用双环介导的等温扩增和侧流试纸法检测和鉴别鸡棘口虫科吸虫和铁线虫属寄生虫感染。

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-01-09 DOI: 10.1016/j.rvsc.2025.105539

Wasin Panich , Thanawan Tejangkura , Thapana Chontananarth

Most poultry farming that has been conducted by smallholders in Thailand are free-range and housing systems, which have risks of parasitic infection from the environment, particularly from tapeworms in the genus Raillietina and trematodes in the family Echinostomatidae. These have become important health problems in the poultry industry, causing low feed conversion ratios and leading to the loss of economic value. Our objective was to develop and validate a molecular technique based on duplex loop-mediated isothermal amplification (LAMP) together with lateral flow dipstick (LFD) assay for discriminating the infections between the genus Raillietina (R. echinobothrida, R. tetragona, and R. cesticillus) and the family Echinostomatidae (E. miyagawai, E. mekongi, E. macrorchis, and H. conoideum) in a single reaction. The developed assay was highly specific without cross-amplification with other poultry helminths and their hosts, at the optimized condition of 66 °C for 80 min. In addition, the results could be clearly visualized with the naked eye via LFD after incubating with probes at 66 °C for 10 min. The detection limit or analytical sensitivity of the Raillietina and Echinostomatidae groups were found to be 5 × 10⁻⁴ and 5 × 10⁻² ng/μL, respectively. In clinical tests, the developed assay successfully detected parasites in naturally infected faeces from chickens in Thailand; results from McNemar's tests revealed no significant difference when compared to standard microscopy methods. Therefore, our assay is a viable alternative technique for an accurate and convenient diagnosis; it can also be used as a tool to guide anthelmintic drugs decision-making for treatments and farm management.

泰国小农经营的大多数家禽养殖都是散养和圈养系统，存在来自环境的寄生虫感染风险，特别是来自细纹绦虫属的绦虫和棘口虫科的吸虫。这些已成为家禽业中重要的健康问题，造成饲料转化率低，并导致经济价值的损失。我们的目的是建立和验证一种基于双环介导的等温扩增（LAMP）和横向流动试纸（LFD）技术的分子技术，用于在单一反应中区分棘孔虫属（棘孔虫属、龙眼虫属和刺孔虫属）和棘孔虫科（miyagawai棘孔虫属、mekongi棘孔虫属、macroorchis棘孔虫科和conoideum棘孔虫科）。在66°C孵育80 min的最佳条件下，该方法与其他家禽蠕虫及其宿主具有高度特异性，无交叉扩增，且探针在66°C孵育10 min后，通过LFD可以清晰地肉眼观察到结果。铁纹虫和棘孔虫组的检测限和分析灵敏度分别为5 × 10-4和5 × 10-2 ng/μL。在临床试验中，开发的分析方法成功地在泰国鸡的自然感染粪便中检测出寄生虫；与标准显微镜方法相比，McNemar的测试结果显示没有显著差异。因此，我们的分析是一个可行的替代技术，准确和方便的诊断；它还可以作为指导驱虫药治疗和农场管理决策的工具。

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引用次数: 0

Biochemical targets of chick embryos affected by sub-lethal concentrations of lambda-cyhalothrin and imidacloprid 亚致死浓度氯氰菊酯和吡虫啉对鸡胚生化指标的影响。

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-01-08 DOI: 10.1016/j.rvsc.2025.105538

Nagat M. Aly, Anter K. Mahmoud, Eman M. Mosallam

The insecticides Lambda-cyhalothrin (LCT) and imidacloprid (IMD) are extensively utilized in Egyptian agriculture. Embryonic chicken is a readily accessible model organism commonly employed in various studies. Eggs of (Gallus Gallus) chicken were immersed in an aqueous solution of two sub-lethal concentrations (0.375 and 0.0375 mg/L for LCT; 0.05 and 0.005 mg/L for IMD) for 30 sec on the fourth day of incubation of chick embryos. Significant reductions of acetylcholinesterase (AChE) activity of brain 18- and 21-day chicks were observed in the groups treated with LCT and IMD dependent on concentrations. There were significant changes (reduction or enhancement) in serum activity of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) by LCT and IMD at the used concentrations, while non-significant stimulation in the AST/ALT ratio (AAR) was found. In 18th - day embryos, the activities of glutathione S-transferase (GST) and acid phosphatase (ACP) were not significantly changed by LCT but were significantly increased by IMD. Liver alkaline phosphatase (ALP) activity showed no significant change except IMD at 0.05 mg/L. However, serum enzyme activity was significantly reduced in all groups. In addition, the tested insecticides caused notable increases in the creatinine and total protein content. The protein profile; proteins separation with sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed an increase in four bands that are consistent with the results of the biomarkers level. Findings indicate that even the pesticide's low concentrations are not safe and may lead to severe damage to the embryos and may lead to significant harm or developmental disruption in the embryos.

杀虫剂高效氯氟氰菊酯（LCT）和吡虫啉（IMD）在埃及农业中被广泛使用。胚胎鸡是一种容易接近的模式生物，通常用于各种研究。将（Gallus Gallus）鸡蛋浸泡在LCT浓度分别为0.375和0.0375 mg/L的亚致死浓度水溶液中；0.05和0.005 mg/L （IMD）在鸡胚孵育第4天孵育30秒。LCT和IMD处理组18日龄和21日龄雏鸡脑乙酰胆碱酯酶（AChE）活性显著降低。LCT和IMD可显著改变（降低或增强）大鼠血清中谷草转氨酶（AST）和丙氨酸转氨酶（ALT）活性，而对AST/ALT比值（AAR）无显著影响。在第18天胚胎中，LCT对谷胱甘肽s转移酶（GST）和酸性磷酸酶（ACP）活性无显著影响，而IMD显著提高了GST和ACP活性。肝脏碱性磷酸酶（ALP）活性除IMD为0.05 mg/L外无显著变化。然而，血清酶活性在所有组中均显著降低。此外，杀虫剂还显著提高了肌酸酐和总蛋白含量。蛋白质谱；十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）显示4个条带增加，与生物标志物水平的结果一致。研究结果表明，即使是低浓度的农药也不安全，可能对胚胎造成严重损害，并可能对胚胎造成重大伤害或发育中断。

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引用次数: 0

Integrative phenotypic and genomic analysis of extended-spectrum Beta-lactamase (ESBL) and carbapenemase genes in Enterobacteriaceae and Pseudomonaceae strains isolated from animals in a Spanish Veterinary Teaching Hospital 西班牙兽医教学医院肠杆菌科和假单胞菌科动物分离菌株扩展谱β -内酰胺酶（ESBL）和碳青霉烯酶基因的综合表型和基因组分析

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-01-08 DOI: 10.1016/j.rvsc.2025.105529

Laura Leal-Vélez , Sergio Quevedo-Caraballo , Raffaele Scarpellini , Marta E. García , José L. Blanco

Antimicrobial resistance (AMR) is a major global health threat, exacerbated by globalization which facilitates the spread of resistant bacteria. Addressing this issue requires a One Health perspective, involving humans, animals, and the environment. This study aims to compare the phenotypic resistance profiles of 69 clinical bacterial isolates (Enterobacteriaceae and Pseudomonaceae) from a Veterinary Teaching Hospital in Spain with their genotypic resistance profiles based on the presence of Extended-Spectrum Beta-Lactamases (ESBLs), AmpC and carbapenemases -enconding genes. For the genotypical analysis, whole genome sequencing (WGS) was used. Phenotypic characterization revealed that 37 isolates (53.6 %) grew on ESBL-selective medium. Phenotypic confirmatory tests showed that 12 strains (17.4 %) had some type of ESBL and 21 (30.4 %) could have an AmpC. Also, 24 isolates (34.8 %) grew in selective media for carbapenemases-producing bacteria, and 2 of these had a class A carbapenemase based on the KPC&MBL&OXA-48 disc kit. The genotypic analysis revealed 20 isolates (29 %) had bla_TEM, 8 (11.6 %) had bla_CTX-M and 7 (10.1 %) bla_SHV. 27 (39.1 %) isolates had class C beta-lactamase genes. 35 isolates (50.7 %) had bla_OXA, class D beta-lactamase. 37 strains (53.6 %) had an Inc. plasmid replicon associated with the spread of AMR genes, including beta-lactamases and carbapenemases. This study emphasizes the value of combining phenotypic and genomic analyses to better understand and address antibiotic resistance, especially in veterinary contexts. Integrating these approaches enhances diagnostic accuracy by identifying strains with resistance genes that may not show phenotypically, helping clinicians in anticipating resistance under selective pressure.

抗菌药耐药性（AMR）是全球健康面临的一大威胁，而全球化又加剧了耐药细菌的传播。要解决这一问题，需要从 "一体健康 "的角度出发，将人类、动物和环境都纳入其中。本研究旨在比较来自西班牙一家兽医教学医院的 69 株临床细菌分离物（肠杆菌科和假单胞菌科）的表型耐药性特征及其基因型耐药性特征，这些特征基于是否存在广谱β-内酰胺酶（ESBLs）、AmpC 和碳青霉烯酶相关基因。基因型分析采用了全基因组测序技术（WGS）。表型特征分析显示，37 个分离株（53.6%）在 ESBL 选择性培养基上生长。表型确认测试表明，12 株（17.4%）具有某种 ESBL，21 株（30.4%）可能具有 AmpC。此外，24 株分离菌（34.8%）在产碳青霉烯酶细菌的选择性培养基中生长，根据 KPC&MBL&OXA-48 盘试剂盒，其中 2 株具有 A 类碳青霉烯酶。基因型分析显示，20 个分离株（29%）带有 blaTEM，8 个分离株（11.6%）带有 blaCTX-M，7 个分离株（10.1%）带有 blaSHV。27个分离株（39.1%）带有C类β-内酰胺酶基因。35 株（50.7%）分离菌株含有 blaOXA（D 类 beta-内酰胺酶）。37株菌株（53.6%）具有与AMR基因（包括β-内酰胺酶和碳青霉烯酶）传播相关的Inc.这项研究强调了结合表型和基因组分析来更好地了解和解决抗生素耐药性问题的价值，尤其是在兽医领域。将这些方法结合起来可提高诊断的准确性，因为它们能识别出表型上可能不明显的带有耐药基因的菌株，从而帮助临床医生预测在选择性压力下的耐药性。

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引用次数: 0

Seronegativity of Iberian ibex (Capra pyrenaica) against Toxoplasma gondii and Neospora caninum is consistent with eco-epidemiological and environmental features in Mediterranean mountainous areas 伊比利亚野山羊（Capra pyrenaica）对刚地弓形虫和犬新孢子虫血清阴性与地中海山区生态流行病学和环境特征一致。

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science

Pub Date : 2025-01-07 DOI: 10.1016/j.rvsc.2025.105530

Alejandro Cano-Manuel , José Enrique Granados , Gema Álvarez-García , Ana Huertas-López , Carlos Diezma-Díaz , Francisco Javier Cano-Manuel , Luis Miguel Ortega-Mora , Paulino Fandos , Gregorio Mentaberre , Jorge Ramón López-Olvera , Carlos Martínez-Carrasco

Knowledge of pathogen epidemiological dynamics and habitat ecological features is essential for wildlife population and health monitoring and management. Toxoplasma gondii and Neospora caninum are two broadly distributed multi-host parasites that affect both wild and domestic animals and, in the case of T. gondii, cause zoonosis. This study reports the seroprevalence of both parasites in Iberian ibex (Capra pyrenaica), a mountain wild ruminant native to the Iberian Peninsula, from the Natural Space of Sierra Nevada (NSSN) in southeastern Spain. Serum from 146 Iberian ibexes were analysed using two in-house ELISA techniques. The positive and doubtful sera were further checked by Western Blot (WB). Seventeen ibexes (11.6 %; 95 % confidence interval 6.4–16.7) were positive for T. gondii and seven (4.8 %; 95 % confidence interval 1.3–8.2) for N. caninum. However, no sera were positive to T. gondii nor to N. caninum by WB. Using at least two different serological techniques is recommended when they are not validated for the target host species. The NSSN is a hypoendemic area for T. gondii and N. caninum, probably determined by the reduced abundance and restricted distribution of their definitive hosts. This would explain the hypoendemic situation in the NSSN and the lack of specific antibodies against these two parasites in the Iberian ibex population. This eco-epidemiological scenario can be challenged by climate and anthropogenic changes, recommending long-term monitoring Iberian ibex population and health, both as a conservation measure for the species and as an indicator of the potential impact of global change on high mountain ecosystems.

了解病原体流行病学动态和生境生态特征对野生动物种群和健康监测与管理至关重要。刚地弓形虫和犬新孢子虫是两种广泛分布的多宿主寄生虫，可影响野生动物和家畜，在刚地弓形虫的情况下，可引起人畜共患病。本研究报告了这两种寄生虫在西班牙东南部内华达山脉自然空间（NSSN）的伊比利亚半岛原产于伊比利亚半岛的山地野生反刍动物伊比利亚山羊（Capra pyrenaica）的血清阳性率。采用两种ELISA技术对146只伊比利亚野山羊的血清进行了分析。采用Western Blot （WB）检测阳性血清和可疑血清。17只野山羊（11.6%）；95%置信区间为6.4 ~ 16.7)，7例(4.8%；95%置信区间1.3-8.2)。血清中弓形虫和犬奈瑟菌均未检测出WB阳性。如果未对目标宿主物种进行验证，建议使用至少两种不同的血清学技术。NSSN是弓形虫和犬奈虫的低流行区，可能是由它们的最终宿主丰度降低和分布受限所决定的。这可以解释伊比利亚山羊种群中NSSN的低流行情况和缺乏针对这两种寄生虫的特异性抗体。这一生态流行病学设想可能受到气候和人为变化的挑战，建议长期监测伊比利亚野山羊的种群和健康，既作为物种的保护措施，也作为全球变化对高山生态系统潜在影响的一个指标。

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引用次数: 0