Pub Date : 2026-03-01Epub Date: 2025-12-15DOI: 10.1016/j.rvsc.2025.106026
M.L. Holmbjerg , C.K. Tvedsborg , M.B.M. Nielsen, J.E. Miles
Despite widespread use of tibial plateau leveling osteotomy (TPLO), the influence of partial medial collateral ligament transection (pMCLX) and physiologic loading on rotational stability remains unclear. This cadaveric study quantified internal and external tibial rotation in canine stifles under simulated weightbearing conditions. Ten unpaired pelvic limbs were mounted in a custom limb press applying axial loads equivalent to 30 % body weight with the stifle fixed at 135°. Quadriceps tension was simulated using a load cell and turnbuckle, and controlled internal and external torques (0.007 Nm/kg) were applied twice per condition. Joints were tested sequentially: intact, after pMCLX at the level of the planned TPLO osteotomy, following TPLO (target tibial plateau angle 5°), and after cranial cruciate ligament transection (CCLX). Internal rotation changed minimally after pMCLX (P = 1.0) but increased significantly following TPLO (P = 0.001) and CCLX (P = 0.002), with limited changes following CCLX (P = 1.0). External rotation increased significantly only between TPLO and CCLX (P = 0.02). These findings indicate that pMCLX does not contribute to significant rotational laxity, while TPLO increases internal rotation independently of CCL integrity. The stabilizing effects of quadriceps tension and axial loading highlight the importance of physiologic constructs in ex vivo models. Overall, these results challenge concerns about iatrogenic instability from pMCLX and support current TPLO positioning strategies.
{"title":"Femorotibial joint rotation in intact joints and following partial medial collateral ligament transection, tibial plateau leveling osteotomy, and cranial cruciate ligament transection – A limb press study","authors":"M.L. Holmbjerg , C.K. Tvedsborg , M.B.M. Nielsen, J.E. Miles","doi":"10.1016/j.rvsc.2025.106026","DOIUrl":"10.1016/j.rvsc.2025.106026","url":null,"abstract":"<div><div>Despite widespread use of tibial plateau leveling osteotomy (TPLO), the influence of partial medial collateral ligament transection (pMCLX) and physiologic loading on rotational stability remains unclear. This cadaveric study quantified internal and external tibial rotation in canine stifles under simulated weightbearing conditions. Ten unpaired pelvic limbs were mounted in a custom limb press applying axial loads equivalent to 30 % body weight with the stifle fixed at 135°. Quadriceps tension was simulated using a load cell and turnbuckle, and controlled internal and external torques (0.007 Nm/kg) were applied twice per condition. Joints were tested sequentially: intact, after pMCLX at the level of the planned TPLO osteotomy, following TPLO (target tibial plateau angle 5°), and after cranial cruciate ligament transection (CCLX). Internal rotation changed minimally after pMCLX (<em>P</em> = 1.0) but increased significantly following TPLO (<em>P</em> = 0.001) and CCLX (<em>P</em> = 0.002), with limited changes following CCLX (<em>P</em> = 1.0). External rotation increased significantly only between TPLO and CCLX (<em>P</em> = 0.02). These findings indicate that pMCLX does not contribute to significant rotational laxity, while TPLO increases internal rotation independently of CCL integrity. The stabilizing effects of quadriceps tension and axial loading highlight the importance of physiologic constructs in ex vivo models. Overall, these results challenge concerns about iatrogenic instability from pMCLX and support current TPLO positioning strategies.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106026"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145792136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2026-01-05DOI: 10.1016/j.rvsc.2026.106057
K.S. Kamalesh Kumar , Rohit Kumar , T. Sai Kumar , Aman Kumar Tiwari , Abhishek C. Saxena , O.R. Vinodh Kumar , Bhanu Pratap Singh , Abhijit Pawde , Amarpal
Cemented total hip replacement (THR) remains an established procedure for restoring hip joint function in dogs with degenerative or traumatic coxofemoral disease; however, limited evidence exists regarding the mechanical integrity and load-transfer characteristics of cemented femoral stem constructs within native canine femora. This study critically evaluated the biomechanical performance of a novel modular cemented femoral stem using cadaveric femora from German Shepherd Dogs (GSDs) and Labrador Retrievers (LABs), emphasizing axial, bending, and torsional behaviors in correlation with radiographic and morphometric parameters. Eighteen pairs of adult canine femora were harvested postmortem, with one side implanted following standardized second-generation cementation protocols and the contralateral side retained intact. Following radiographic and CT-based templating, specimens underwent displacement-controlled axial compression, three-point bending, and torsion tests. Cemented implantation significantly reduced ultimate compressive, bending, and torsional strengths compared to intact femora (p < 0.05), though stiffness remained unaffected, suggesting preservation of elastic response despite altered failure thresholds. GSD femora consistently exhibited greater load-bearing capacity, yield strength, and energy absorption than LABs, reflecting breed-specific variations in cortical geometry and canal morphology. Radiographs confirmed precise stem alignment, uniform cement mantles averaging 5 mm, and consistent canal fill exceeding 60 %, indicative of optimized cementation. Failure patterns transitioned from brittle cortical fractures in intact specimens to ductile interfacial shear at the bone-cement interface in implanted constructs, demonstrating efficient load redistribution through the composite system. Collectively, these findings highlight the mechanical reliability and breed-dependent adaptability of the cemented THR system, supporting its translational potential for achieving durable fixation and functional load transfer in canine clinical applications while offering valuable insights relevant to comparative orthopedic biomechanics.
{"title":"Biomechanical evaluation of a cemented femoral stem for canine total hip replacement across two breeds","authors":"K.S. Kamalesh Kumar , Rohit Kumar , T. Sai Kumar , Aman Kumar Tiwari , Abhishek C. Saxena , O.R. Vinodh Kumar , Bhanu Pratap Singh , Abhijit Pawde , Amarpal","doi":"10.1016/j.rvsc.2026.106057","DOIUrl":"10.1016/j.rvsc.2026.106057","url":null,"abstract":"<div><div>Cemented total hip replacement (THR) remains an established procedure for restoring hip joint function in dogs with degenerative or traumatic coxofemoral disease; however, limited evidence exists regarding the mechanical integrity and load-transfer characteristics of cemented femoral stem constructs within native canine femora. This study critically evaluated the biomechanical performance of a novel modular cemented femoral stem using cadaveric femora from German Shepherd Dogs (GSDs) and Labrador Retrievers (LABs), emphasizing axial, bending, and torsional behaviors in correlation with radiographic and morphometric parameters. Eighteen pairs of adult canine femora were harvested postmortem, with one side implanted following standardized second-generation cementation protocols and the contralateral side retained intact. Following radiographic and CT-based templating, specimens underwent displacement-controlled axial compression, three-point bending, and torsion tests. Cemented implantation significantly reduced ultimate compressive, bending, and torsional strengths compared to intact femora (<em>p</em> < 0.05), though stiffness remained unaffected, suggesting preservation of elastic response despite altered failure thresholds. GSD femora consistently exhibited greater load-bearing capacity, yield strength, and energy absorption than LABs, reflecting breed-specific variations in cortical geometry and canal morphology. Radiographs confirmed precise stem alignment, uniform cement mantles averaging 5 mm, and consistent canal fill exceeding 60 %, indicative of optimized cementation. Failure patterns transitioned from brittle cortical fractures in intact specimens to ductile interfacial shear at the bone-cement interface in implanted constructs, demonstrating efficient load redistribution through the composite system. Collectively, these findings highlight the mechanical reliability and breed-dependent adaptability of the cemented THR system, supporting its translational potential for achieving durable fixation and functional load transfer in canine clinical applications while offering valuable insights relevant to comparative orthopedic biomechanics.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"201 ","pages":"Article 106057"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145934710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to isolate and identify Escherichia coli (E. coli) from the feces of wild bird species and to determine antibiotic susceptibilities, phylogroups, and virulence genes in the isolates obtained.
For this purpose, a total of 575 fecal samples from 100 Eurasian Tree Sparrow, 250 Eurasian Jackdaw, 156 Eurasian Magpie, 4 Short-toed Snake-Eagle, 60 Rock dove, and 5 Long-legged Buzzard were used. E. coli isolation was performed using direct inoculation on MacConkey agar. The isolates were identified through phenotypic tests, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF-MS), and Polymerase Chain Reaction (PCR). The susceptibility of the isolates to nine antibiotics was determined by the disk diffusion method. Phylogroups and the presence of virulence genes were determined by Clermont typing and the multiplex PCR (mPCR) methods, respectively. Among 575 feces samples, E. coli was isolated from 37 (6.43 %). The highest antibiotic resistance was found in ampicillin, azithromycin, and trimethoprim-sulfamethoxazole at 35.1 %. Moreover, 43.2 % of the E. coli isolates were detected as Multidrug-Resistant (MDR), and these isolates exhibited 13 different MDR patterns by demonstrating resistance to three to eight various antibiotic classes. While the most common phylogroup detected among isolates was A (18.9 %), none belonged to phylogroups C and F. 72.9 % had at least one virulence gene. The most frequently detected virulence gene was stx1 + stx2 (32.4 %), and all isolates were negative for bfpA and lt genes.
In conclusion, considering the role of birds of prey in the ecological balance, the data obtained in this study suggest that wild birds may contribute to the spread of pathogenic and antibiotic-resistant E. coli strains globally.
{"title":"Escherichia coli strains from wild birds in Türkiye: Antibiotic resistance, virulence factors and phylogroups","authors":"Doğancan Yarım, Emre Karakaya, Fuat Aydın, Seçil Abay","doi":"10.1016/j.rvsc.2025.106045","DOIUrl":"10.1016/j.rvsc.2025.106045","url":null,"abstract":"<div><div>This study aimed to isolate and identify <em>Escherichia coli</em> (<em>E. coli</em>) from the feces of wild bird species and to determine antibiotic susceptibilities, phylogroups, and virulence genes in the isolates obtained.</div><div>For this purpose, a total of 575 fecal samples from 100 Eurasian Tree Sparrow, 250 Eurasian Jackdaw, 156 Eurasian Magpie, 4 Short-toed Snake-Eagle, 60 Rock dove, and 5 Long-legged Buzzard were used. <em>E. coli</em> isolation was performed using direct inoculation on MacConkey agar. The isolates were identified through phenotypic tests, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF-MS), and Polymerase Chain Reaction (PCR). The susceptibility of the isolates to nine antibiotics was determined by the disk diffusion method. Phylogroups and the presence of virulence genes were determined by Clermont typing and the multiplex PCR (mPCR) methods, respectively. Among 575 feces samples, <em>E. coli</em> was isolated from 37 (6.43 %). The highest antibiotic resistance was found in ampicillin, azithromycin, and trimethoprim-sulfamethoxazole at 35.1 %. Moreover, 43.2 % of the <em>E. coli</em> isolates were detected as Multidrug-Resistant (MDR), and these isolates exhibited 13 different MDR patterns by demonstrating resistance to three to eight various antibiotic classes. While the most common phylogroup detected among isolates was A (18.9 %), none belonged to phylogroups C and F. 72.9 % had at least one virulence gene. The most frequently detected virulence gene was <em>stx1</em> + <em>stx2</em> (32.4 %), and all isolates were negative for <em>bfpA</em> and <em>lt</em> genes.</div><div>In conclusion, considering the role of birds of prey in the ecological balance, the data obtained in this study suggest that wild birds may contribute to the spread of pathogenic and antibiotic-resistant <em>E. coli</em> strains globally.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106045"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145864882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2025-12-17DOI: 10.1016/j.rvsc.2025.106034
Paula Yagüez I. López-Jurado , Katie Beckmann , Rob Kelly
The European hedgehog (Erinaceus europaeus) is prevalent across Western Europe, with many individuals admitted to wildlife rehabilitation centres. Parasitic infections, particularly helminth parasites, may threaten rehabilitation success. This study investigated respiratory and gastrointestinal endoparasite prevalence, risk factors, and associations with survival in 300 hedgehogs admitted to four rehabilitation centres in northwestern France (May 2023–May 2024). Faecal samples were analysed using either the modified McMaster technique (Oise) or direct smear (Seine-Maritime, Loir-et-Cher, Essonne). Overall, 58.3 % of hedgehogs carried at least one endoparasite. The most common were Capillaria species group (43.6 %) and Crenosoma striatum (25.7 %), followed by Brachylaemus erinacei (11 %) and Cycloisospora species group (7.3 %). Co-infection was common; 35 % of infected individuals harboured both Capillaria spp. and C. striatum. Younger hedgehogs showed lower prevalence but higher parasite burdens, suggesting reduced resistance. Capillaria spp. prevalence peaked in winter across all age groups. Multivariable analysis identified age, weight, season, and admission reason as significant risk factors for parasitism. Importantly, detection of C. striatum significantly increased mortality risk during rehabilitation (OR = 3.44, p = 0.0002). These findings highlight the need for targeted parasite screening and treatment protocols for specific risk groups, for example for juveniles and individuals with clinical signs. Coprological analysis offers a practical, non-invasive tool for parasite detection in resource-limited rehabilitation settings. This is the first study to report endoparasite prevalence in live hedgehogs in France using coprological methods and to demonstrate a statistical association between C. striatum infection and mortality in rehabilitation.
欧洲刺猬（Erinaceus europaeus）在西欧普遍存在，许多个体被送入野生动物康复中心。寄生虫感染，特别是蠕虫寄生虫，可能威胁康复的成功。本研究调查了法国西北部4个康复中心（2023年5月至2024年5月）入院的300只刺猬的呼吸道和胃肠道内寄生虫的流行情况、危险因素及其与生存率的关系。使用改良的麦克马斯特技术（Oise）或直接涂片（Seine-Maritime, Loir-et-Cher, Essonne）对粪便样本进行分析。总体而言，58.3%的刺猬携带至少一种内寄生虫。最常见的是毛缕虫（43.6%）和纹状毛蕊虫（25.7%），其次是毛缕虫（11%）和环异孢子虫（7.3%）。合并感染较为常见；35%的受感染个体同时携带毛线虫和纹状体线虫。年轻的刺猬患病率较低，但寄生虫负担较高，表明抗性降低。在所有年龄组中，毛细线虫的患病率在冬季达到高峰。多变量分析发现年龄、体重、季节和入院原因是寄生虫病的重要危险因素。重要的是，纹状体的检测显著增加康复期间的死亡风险（OR = 3.44, p = 0.0002）。这些发现强调需要针对特定风险群体，例如青少年和有临床症状的个体，制定有针对性的寄生虫筛查和治疗方案。在资源有限的康复环境中，coprology分析为寄生虫检测提供了一种实用的、非侵入性的工具。这是第一个使用粪学方法报道法国活刺猬体内寄生虫流行情况的研究，并证明纹状体感染与康复死亡率之间存在统计学关联。
{"title":"Epidemiology of endoparasite infections in the European hedgehog (Erinaceus europaeus) and association with rehabilitation outcome in wildlife centres in Northwest France","authors":"Paula Yagüez I. López-Jurado , Katie Beckmann , Rob Kelly","doi":"10.1016/j.rvsc.2025.106034","DOIUrl":"10.1016/j.rvsc.2025.106034","url":null,"abstract":"<div><div>The European hedgehog (<em>Erinaceus europaeus</em>) is prevalent across Western Europe, with many individuals admitted to wildlife rehabilitation centres. Parasitic infections, particularly helminth parasites, may threaten rehabilitation success. This study investigated respiratory and gastrointestinal endoparasite prevalence, risk factors, and associations with survival in 300 hedgehogs admitted to four rehabilitation centres in northwestern France (May 2023–May 2024). Faecal samples were analysed using either the modified McMaster technique (Oise) or direct smear (Seine-Maritime, Loir-et-Cher, Essonne). Overall, 58.3 % of hedgehogs carried at least one endoparasite. The most common were <em>Capillaria</em> species group (43.6 %) and <em>Crenosoma striatum</em> (25.7 %), followed by <em>Brachylaemus erinacei</em> (11 %) and <em>Cycloisospora</em> species group (7.3 %). Co-infection was common; 35 % of infected individuals harboured both <em>Capillaria</em> spp. and <em>C. striatum</em>. Younger hedgehogs showed lower prevalence but higher parasite burdens, suggesting reduced resistance. <em>Capillaria</em> spp. prevalence peaked in winter across all age groups. Multivariable analysis identified age, weight, season, and admission reason as significant risk factors for parasitism. Importantly, detection of <em>C. striatum</em> significantly increased mortality risk during rehabilitation (OR = 3.44, <em>p</em> = 0.0002). These findings highlight the need for targeted parasite screening and treatment protocols for specific risk groups, for example for juveniles and individuals with clinical signs. Coprological analysis offers a practical, non-invasive tool for parasite detection in resource-limited rehabilitation settings. This is the first study to report endoparasite prevalence in live hedgehogs in France using coprological methods and to demonstrate a statistical association between <em>C. striatum</em> infection and mortality in rehabilitation.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106034"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145766055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2025-12-20DOI: 10.1016/j.rvsc.2025.106037
Jia Wang , Yi-Han Lu , Bo-Han , Hai-Tao Yang , Xin-lei Li , Wen Sun , Shi-Qin Zhang , Zhuo-Ran Miao , Xue-Jiao Cheng , Chun-Xue You , Ying-Feng Sun
Porcine reproductive and respiratory syndrome virus (PRRSV) has imposed significant economic burdens on the global swine industry. The majority of PRRSV strains can induce high fever at various stages of pig development. However, the PRRSV strain that triggers the biphasic temperature phenomenon remains relatively rare. In this study, a novel PRRSV variant, designated SJ0107, was isolated from local pig farms in Tianjin, Northern China, in 2024. Recombination analysis reveals that SJ0107 is a recombinant PRRSV strain resulting from the interaction between lineage 1.8 and lineage 8.3, with four potential recombination breakpoints located in Nsp2 (nt 766/1997), Nsp4 (nt 5413), and Nsp9 (nt 7750). Animal challenge experiments conducted on four-week-old piglets demonstrated that SJ0107 can induce rare initial hypothermia symptoms within 5 days post-inoculation (dpi). Subsequently, the body temperature returned to normal, and high-grade fever symptoms emerged at 11 dpi, lasting for one week before normalizing again. Meanwhile, severe histopathological lung lesions were also detected in the pathological sections. In conclusion, the findings reveal that the SJ0107 strain undergoes a dual-phase temperature alteration, which not only complicates traditional diagnostic criteria reliant on persistent fever but also heightens the risk of misdiagnosis in the initial phases of infection. These insights underscore the necessity of revising surveillance protocols for emerging PRRSV variants that exhibit atypical pathogenic characteristics.
{"title":"Emergence of a novel porcine reproductive and respiratory syndrome virus strain inducing biphasic temperature fluctuations in Tianjin, northern China","authors":"Jia Wang , Yi-Han Lu , Bo-Han , Hai-Tao Yang , Xin-lei Li , Wen Sun , Shi-Qin Zhang , Zhuo-Ran Miao , Xue-Jiao Cheng , Chun-Xue You , Ying-Feng Sun","doi":"10.1016/j.rvsc.2025.106037","DOIUrl":"10.1016/j.rvsc.2025.106037","url":null,"abstract":"<div><div>Porcine reproductive and respiratory syndrome virus (PRRSV) has imposed significant economic burdens on the global swine industry. The majority of PRRSV strains can induce high fever at various stages of pig development. However, the PRRSV strain that triggers the biphasic temperature phenomenon remains relatively rare. In this study, a novel PRRSV variant, designated SJ0107, was isolated from local pig farms in Tianjin, Northern China, in 2024. Recombination analysis reveals that SJ0107 is a recombinant PRRSV strain resulting from the interaction between lineage 1.8 and lineage 8.3, with four potential recombination breakpoints located in Nsp2 (nt 766/1997), Nsp4 (nt 5413), and Nsp9 (nt 7750). Animal challenge experiments conducted on four-week-old piglets demonstrated that SJ0107 can induce rare initial hypothermia symptoms within 5 days post-inoculation (dpi). Subsequently, the body temperature returned to normal, and high-grade fever symptoms emerged at 11 dpi, lasting for one week before normalizing again. Meanwhile, severe histopathological lung lesions were also detected in the pathological sections. In conclusion, the findings reveal that the SJ0107 strain undergoes a dual-phase temperature alteration, which not only complicates traditional diagnostic criteria reliant on persistent fever but also heightens the risk of misdiagnosis in the initial phases of infection. These insights underscore the necessity of revising surveillance protocols for emerging PRRSV variants that exhibit atypical pathogenic characteristics.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106037"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145820587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Risk-based inspection of pigs at the abattoir depends on accurately identifying high-risk animals before slaughter. Food Chain Information (FCI) provides data on farm management, animal health, and treatments that can support this process; however, its limited completeness and reliability highlight the need for complementary approaches. Antemortem (AM) conditions may serve as practical predictors of postmortem (PM) lesions, supporting the development of risk-based inspection systems. This study investigated the potential of AM inspection in detecting PM lesions in finisher pigs, slaughtered at a commercial abattoir in Portugal. Data were collected from 62 batches (n = 9542 pigs) for a cross-sectional study, and 245 pigs for a case control study. Detection of coughing, skin lesions, and growth retardation was significantly higher when pigs were inspected from inside the pens, compared with unloading or outside the pens observations. At batch-level coughing at AM inspection was associated with a higher number of PM lesions, particularly pneumonia (OR = 1.08) and pleurisy (OR = 1.03). At animal-level, AM conditions showed consistent associations with PM lesions, including coughing with pneumonia (OR = 6.7), and lung lesion (OR = 6.2), lameness with arthritis (OR = 14.9) and growth retardation with several PM lesions. These findings are consistent with those reported in other countries and confirm that specific AM conditions can reliably predict certain PM lesions, contributing to the refinement of risk-based inspection approaches in Portugal.
{"title":"A field observational study evaluating antemortem inspection as a predictive tool for postmortem lesion findings at a Portuguese pig abattoir","authors":"Márcia Santos Nunes , Abbey Olsen , Rui Pedro Cordeiro , Madalena Vieira-Pinto","doi":"10.1016/j.rvsc.2025.106043","DOIUrl":"10.1016/j.rvsc.2025.106043","url":null,"abstract":"<div><div>Risk-based inspection of pigs at the abattoir depends on accurately identifying high-risk animals before slaughter. Food Chain Information (FCI) provides data on farm management, animal health, and treatments that can support this process; however, its limited completeness and reliability highlight the need for complementary approaches. Antemortem (AM) conditions may serve as practical predictors of postmortem (PM) lesions, supporting the development of risk-based inspection systems. This study investigated the potential of AM inspection in detecting PM lesions in finisher pigs, slaughtered at a commercial abattoir in Portugal. Data were collected from 62 batches (<em>n</em> = 9542 pigs) for a cross-sectional study, and 245 pigs for a case control study. Detection of coughing, skin lesions, and growth retardation was significantly higher when pigs were inspected from inside the pens, compared with unloading or outside the pens observations. At batch-level coughing at AM inspection was associated with a higher number of PM lesions, particularly pneumonia (OR = 1.08) and pleurisy (OR = 1.03). At animal-level, AM conditions showed consistent associations with PM lesions, including coughing with pneumonia (OR = 6.7), and lung lesion (OR = 6.2), lameness with arthritis (OR = 14.9) and growth retardation with several PM lesions. These findings are consistent with those reported in other countries and confirm that specific AM conditions can reliably predict certain PM lesions, contributing to the refinement of risk-based inspection approaches in Portugal.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"201 ","pages":"Article 106043"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145895943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2025-12-30DOI: 10.1016/j.rvsc.2025.106046
Chenchen Sheng , Jingfang Wang , Mengyuan Tan , jingwen Zhang , Mengran Sun , Jiumeng Sun , Ying Shao , Jian Tu , Liangqiang Zhu , Xiangjun Song
Chicken Infectious Anemia Virus (CIAV) causes chicken infectious anemia, characterized by anemia and immune dysfunction. The rapid dissemination of this virus is generating substantial economic consequences for poultry producers.
The CRISPR/Cas12a system is widely used for virus detection through crRNA-guided target recognition and the paracrine activity of Cas12a. To enable rapid and highly sensitive detection of Chicken Infectious Anemia Virus (CIAV), a CRISPR-Cas12a-based fluorescence assay was refined. Through optimization of the CRISPR/Cas12a system and integration of enzymatic recombinase amplification (ERA), the assay achieved a detection limit of 1 copy/μL, demonstrating its significant utility for CIAV diagnostics. In addition, a CRISPR/Cas12a lateral flow assay was developed and optimized, achieving a sensitivity of 10^3 copies/μL for the rapid and visual detection of target analytes. This technique exhibits high specificity for CIAV, showing no cross-reactivity with other chicken viruses. Overall, the system enables rapid CIAV detection with cost-effective equipment, making it suitable for virus monitoring.
{"title":"Establishment of detection method of chicken infectious anemia virus based on CRISPR/Cas12a system","authors":"Chenchen Sheng , Jingfang Wang , Mengyuan Tan , jingwen Zhang , Mengran Sun , Jiumeng Sun , Ying Shao , Jian Tu , Liangqiang Zhu , Xiangjun Song","doi":"10.1016/j.rvsc.2025.106046","DOIUrl":"10.1016/j.rvsc.2025.106046","url":null,"abstract":"<div><div>Chicken Infectious Anemia Virus (CIAV) causes chicken infectious anemia, characterized by anemia and immune dysfunction. The rapid dissemination of this virus is generating substantial economic consequences for poultry producers.</div><div>The CRISPR/Cas12a system is widely used for virus detection through crRNA-guided target recognition and the paracrine activity of Cas12a. To enable rapid and highly sensitive detection of Chicken Infectious Anemia Virus (CIAV), a CRISPR-Cas12a-based fluorescence assay was refined. Through optimization of the CRISPR/Cas12a system and integration of enzymatic recombinase amplification (ERA), the assay achieved a detection limit of 1 copy/μL, demonstrating its significant utility for CIAV diagnostics. In addition, a CRISPR/Cas12a lateral flow assay was developed and optimized, achieving a sensitivity of 10^3 copies/μL for the rapid and visual detection of target analytes. This technique exhibits high specificity for CIAV, showing no cross-reactivity with other chicken viruses. Overall, the system enables rapid CIAV detection with cost-effective equipment, making it suitable for virus monitoring.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"201 ","pages":"Article 106046"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145918358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Di-n-butyl phthalate (DBP) is an organic compound of phthalate, commonly used in the production of various plastic materials and its detrimental effects on marine ecosystems and human health have a great concern. Bioactive compounds derived from microalgae have numerous biological and pharmacological activities and their remedial role in alleviating the adverse effects of toxicity induced by environmental and other agents has also been reported. Thus, in this work, we carried out studies to ascertain if exopolysaccharides (EPS) extracted from Arthrospira platensis (Cyanobacteria) would ameliorate DBP-induced toxicity in gilthead seabream (Sparus aurata), used as model organism, focusing on growth performance, oxidative stress, neurotoxicity, histopathological changes in liver and intestine, and muscle nutritional quality. Firstly, fish in triplicate were fed separately a supplemented diet with 0 % (control group) and 6 % of EPS (EPS group) for 21 days. Consequently, each group were intraperitoneally exposed to DBP at a concentration of 85 μg /kg body weight. The results showed that DBP impaired growth, induced antioxidant-oxidative stress imbalance and caused genotoxicity and liver and intestine damage. Also, DBP disrupted muscle proximate composition and fatty acid profiles. Interestingly, feeding the DBP-exposed fish with EPS partially restored, histopathological damage in liver and intestine, antioxidant markers, lipid balance, notably improving the ω3/ω6 ratio, likely through EPS's antioxidant and metabolic regulatory effects.
Overall, this study offered further insights on the antioxidative and chemopreventive properties of exopolysaccharides (EPS) extracted from Arthrospira platensis to modulate fish health and to enhance growth and protect against DBP-induced toxicity.
{"title":"Remedial effects of extracellular polymeric substances (EPS) from Arthrospira platensis against Di-n-butyl phthalate (DBP) toxicity on gilthead seabream (Sparus aurata)","authors":"Amira Bemri , Fethi Jebali , Jihene Ammar , Tahar Gharred , Jamel Jebali , Hamadi Guerbej , Hatem Ben Ouada , Zied Bouraoui","doi":"10.1016/j.rvsc.2025.106041","DOIUrl":"10.1016/j.rvsc.2025.106041","url":null,"abstract":"<div><div>Di-n-butyl phthalate (DBP) is an organic compound of phthalate, commonly used in the production of various plastic materials and its detrimental effects on marine ecosystems and human health have a great concern. Bioactive compounds derived from microalgae have numerous biological and pharmacological activities and their remedial role in alleviating the adverse effects of toxicity induced by environmental and other agents has also been reported. Thus, in this work, we carried out studies to ascertain if exopolysaccharides (EPS) extracted from <em>Arthrospira platensis</em> (Cyanobacteria) would ameliorate DBP-induced toxicity in gilthead seabream (<em>Sparus aurata</em>), used as model organism, focusing on growth performance, oxidative stress, neurotoxicity, histopathological changes in liver and intestine, and muscle nutritional quality. Firstly, fish in triplicate were fed separately a supplemented diet with 0 % (control group) and 6 % of EPS (EPS group) for 21 days. Consequently, each group were intraperitoneally exposed to DBP at a concentration of 85 μg /kg body weight. The results showed that DBP impaired growth, induced antioxidant-oxidative stress imbalance and caused genotoxicity and liver and intestine damage. Also, DBP disrupted muscle proximate composition and fatty acid profiles. Interestingly, feeding the DBP-exposed fish with EPS partially restored, histopathological damage in liver and intestine, antioxidant markers, lipid balance, notably improving the ω3/ω6 ratio, likely through EPS's antioxidant and metabolic regulatory effects.</div><div>Overall, this study offered further insights on the antioxidative and chemopreventive properties of exopolysaccharides (EPS) extracted from <em>Arthrospira platensis</em> to modulate fish health and to enhance growth and protect against DBP-induced toxicity.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106041"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145841836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2025-12-31DOI: 10.1016/j.rvsc.2025.106047
Marcilene Daniel Damasceno , Maysa Serpa Gonçalves , Maria Eduarda de Souza Teixeira Campos , Amanda Carvalho Rosado Ferreira , Dircéia Aparecida Costa Custódio , Eduarda Moraes Magossi Silva , Vivian Aparecida Malta , Alice Gonçalves dos Reis , Bruno Borges Silva , Ana Clara de Serpa Carvalho , Júlia Lima Paz , Pedro Felipe Rodrigues e Oliveira , Bruno Campos de Carvalho , Guilherme Nunes Souza , Carine Rodrigues Pereira , Elaine Maria Seles Dorneles , Alessandro de Sá Guimarães
Compost-bedded pack barn (CBP) is a used intensive system for dairy cow confinement, however, information about the possible role of the bed in the maintenance and transmission of pathogens among housed animals is still scarce. This study investigates the presence of Brucella spp. in dairy cows in the transition period housed in CBP and the detection of the pathogen in the CBP. The analyzes were conducted using samples of CBP and samples from cows up to 45 days post-partum from 20 different farms in Goiás and Minas Gerais states, Brazil. Animals were tested for the presence of anti-Brucella antibodies. Samples of vaginal swab, endometrial sample and CBP were tested for the presence of Brucella spp. using conventional PCR targeting the bscp31 gene, as a screening test, and the real-time PCR targeting the IS711 gene, as confirmatory test. A total of 16 [16/20 (80 %)] properties exhibited at least one positive animal in at least one test (serology or molecular tests), and four [4/20 (25 %)] of these properties had at least one positive animal in at least two tests performed. Additionally, three CBP [3/44 (6.88 %)] showed PCR positive result to Brucella spp. The results demonstrated the presence of Brucella spp. in sample collected from dairy cows in transition period housed in CBP and the bed of the system, suggesting the CBP may have a role in brucellosis transmission in the system, by favoring the spread of the agent and transmission to other housed cows.
{"title":"Detection of Brucella spp. in compost-bedding pack barn and its relationship with infections in dairy cows during the transition period","authors":"Marcilene Daniel Damasceno , Maysa Serpa Gonçalves , Maria Eduarda de Souza Teixeira Campos , Amanda Carvalho Rosado Ferreira , Dircéia Aparecida Costa Custódio , Eduarda Moraes Magossi Silva , Vivian Aparecida Malta , Alice Gonçalves dos Reis , Bruno Borges Silva , Ana Clara de Serpa Carvalho , Júlia Lima Paz , Pedro Felipe Rodrigues e Oliveira , Bruno Campos de Carvalho , Guilherme Nunes Souza , Carine Rodrigues Pereira , Elaine Maria Seles Dorneles , Alessandro de Sá Guimarães","doi":"10.1016/j.rvsc.2025.106047","DOIUrl":"10.1016/j.rvsc.2025.106047","url":null,"abstract":"<div><div>Compost-bedded pack barn (CBP) is a used intensive system for dairy cow confinement, however, information about the possible role of the bed in the maintenance and transmission of pathogens among housed animals is still scarce. This study investigates the presence of <em>Brucella</em> spp. in dairy cows in the transition period housed in CBP and the detection of the pathogen in the CBP. The analyzes were conducted using samples of CBP and samples from cows up to 45 days post-partum from 20 different farms in Goiás and Minas Gerais states, Brazil. Animals were tested for the presence of anti-<em>Brucella</em> antibodies. Samples of vaginal swab, endometrial sample and CBP were tested for the presence of <em>Brucella</em> spp. using conventional PCR targeting the <em>bscp31</em> gene, as a screening test, and the real-time PCR targeting the <em>IS711</em> gene, as confirmatory test. A total of 16 [16/20 (80 %)] properties exhibited at least one positive animal in at least one test (serology or molecular tests), and four [4/20 (25 %)] of these properties had at least one positive animal in at least two tests performed. Additionally, three CBP [3/44 (6.88 %)] showed PCR positive result to <em>Brucella</em> spp. The results demonstrated the presence of <em>Brucella</em> spp. in sample collected from dairy cows in transition period housed in CBP and the bed of the system, suggesting the CBP may have a role in brucellosis transmission in the system, by favoring the spread of the agent and transmission to other housed cows.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"201 ","pages":"Article 106047"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145940801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2026-01-01DOI: 10.1016/j.rvsc.2025.106048
Alfredo Di Lucrezia , Daria Lotito , Valeria Iervolino , Pietro Lombardi , Biagio D'Aniello , Vincenzo Mastellone
Social behaviors are widely recognized as valuable indicators of animal welfare. This study examined the behavioral profiles of two groups of cows: one with daytime access to an open outdoor area and one housed exclusively in a free-stall barn. Observations were conducted when both groups were in the same indoor environment, allowing for a direct comparison of their behavioral expressions. Cows with outdoor access engaged significantly more in social play, while no statistically significant differences were observed for other social behaviors such as allogrooming, social rubbing, or submission/avoidance. Since play behavior typically emerges under favorable psychological conditions, these findings suggest that outdoor access during the day contributes to enhanced psychological wellbeing, with positive effects that persist even when cows return to the confined barn setting. In contrast, negative social interactions such as submission/ avoidance were expressed at similar levels across both groups, indicating that access to open space may not be sufficient to mitigate all forms of social stress.
{"title":"The availability of outdoor spaces enhances social play in dairy cows","authors":"Alfredo Di Lucrezia , Daria Lotito , Valeria Iervolino , Pietro Lombardi , Biagio D'Aniello , Vincenzo Mastellone","doi":"10.1016/j.rvsc.2025.106048","DOIUrl":"10.1016/j.rvsc.2025.106048","url":null,"abstract":"<div><div>Social behaviors are widely recognized as valuable indicators of animal welfare. This study examined the behavioral profiles of two groups of cows: one with daytime access to an open outdoor area and one housed exclusively in a free-stall barn. Observations were conducted when both groups were in the same indoor environment, allowing for a direct comparison of their behavioral expressions. Cows with outdoor access engaged significantly more in social play, while no statistically significant differences were observed for other social behaviors such as allogrooming, social rubbing, or submission/avoidance. Since play behavior typically emerges under favorable psychological conditions, these findings suggest that outdoor access during the day contributes to enhanced psychological wellbeing, with positive effects that persist even when cows return to the confined barn setting. In contrast, negative social interactions such as submission/ avoidance were expressed at similar levels across both groups, indicating that access to open space may not be sufficient to mitigate all forms of social stress.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"201 ","pages":"Article 106048"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145940802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号