Pub Date : 2020-01-01DOI: 10.22127/RJP.2019.168142.1462
M. Azadbakht, A. Chabra, A. Akbarabadi, M. Motazedian, Taha Monadi, F. Akbari
Background and objectives: Giardiasis and amoebiasis are two common diseases in human societies which make increasing problems for the health managing systems. In the present study, the antiparasitic activity of Allium sativum, Artemisia sieberi, Zatraria multiflora, Chenopodium botrys and Eucalyptus globulus essential oils on Giardia lamblia cysts and Entamoeba histolytica trophozoites were investigated in vitro. Methods: Giardia lamblia cysts and E. histolytica trophozoites were isolated from infected sample stools. The parasites were treated by four concentrations (0.2, 0.1, 0.01 and 0.001 µg/mL) of essential oils, separately for 30 min at 37 °C. Results: Theessential oils at the concentration of 0.1 and 0.2 µg/mL indicated appreciate parasiticidal effect (p<0.05). Eucalyptus globulus had maximum efficacy on both G. lamblia and E. histolytica with mortality rates of 79.7% and 87.6% within 30 min. Conclusion: All essential oils showed potent anti-giardiasis and anti-amoebiasis activity. Also, E. globulus with the highest efficiency could be considered as anti-protozoa medication to use an adjunct or primary therapy.
{"title":"Anti-parasitic Activity of Some Medicinal Plants Essential Oils on Giardia lamblia and Entamoeba histolytica, In Vitro","authors":"M. Azadbakht, A. Chabra, A. Akbarabadi, M. Motazedian, Taha Monadi, F. Akbari","doi":"10.22127/RJP.2019.168142.1462","DOIUrl":"https://doi.org/10.22127/RJP.2019.168142.1462","url":null,"abstract":"Background and objectives: Giardiasis and amoebiasis are two common diseases in human societies which make increasing problems for the health managing systems. In the present study, the antiparasitic activity of Allium sativum, Artemisia sieberi, Zatraria multiflora, Chenopodium botrys and Eucalyptus globulus essential oils on Giardia lamblia cysts and Entamoeba histolytica trophozoites were investigated in vitro. Methods: Giardia lamblia cysts and E. histolytica trophozoites were isolated from infected sample stools. The parasites were treated by four concentrations (0.2, 0.1, 0.01 and 0.001 µg/mL) of essential oils, separately for 30 min at 37 °C. Results: Theessential oils at the concentration of 0.1 and 0.2 µg/mL indicated appreciate parasiticidal effect (p<0.05). Eucalyptus globulus had maximum efficacy on both G. lamblia and E. histolytica with mortality rates of 79.7% and 87.6% within 30 min. Conclusion: All essential oils showed potent anti-giardiasis and anti-amoebiasis activity. Also, E. globulus with the highest efficiency could be considered as anti-protozoa medication to use an adjunct or primary therapy.","PeriodicalId":21088,"journal":{"name":"Research Journal of Pharmacognosy","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68212369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.22127/RJP.2019.190642.1506
M. S. Dinani, Narges Zakeri Tehrani, F. Shafiee
Background and objectives: Cancer is a major health problem in the world. The aim of this study was to extract the flowers of Allium austroiranicum used by Iranian people as a condiment or for its medicinal effects followed by bioassay guided fractionation of the extracts and fractions, using anti-proliferative effects against ovarian and cervical cancer cells. Methods: The air-dried flowers of Allium austroiranicum were extracted in a four-step extraction method, resulting hexan, chloroform, chloroform: methanol (9: 1), butanol and aqueous extracts. Anti-proliferative effects of the extracts were evaluated by MTT assay against OVCAR-3, HeLa, and HUVEC cell lines. The most potent cytotoxic extract was then subjected to fractionation by MPLC method on a RP-18 silicagel column. Finally, the cytotoxic effects of resulted fractions were analyzed again and the most potent cytotoxic fraction and its IC50 were determined. Results: Statistical analysis showed thatbutanol extract of A. austroiranicum showed the most potent cytotoxic effects against OVCAR-3, HeLa and HUVEC cell lines with IC50 values of 38±2, 56±1.4, and 60±3.5 µg/mL, respectively. On the other hand, for 7 fractions resulting from fractionation of the butanol extract, MTT assay results showed that 6th fraction (F) was the most cytotoxic fraction with IC50 of 2.7±0.26 and 7.5±0.5 µg/mL for OVCAR-3 and HeLa cancer cell lines, respectively. Primary evaluation of the fraction by TLC and NMR analysis suggested the steroidal saponins as the main constituents. Conclusion: Allium austroiranicum showed significant cytotoxic effects against ovarian cancer cell line especiallyfractions assumed to contain steroidal saponins. The fraction constituents have the potential of being strong cytotoxic agents and the isolation and identification of compounds are suggested.
{"title":"Bioassay Guided Fractionation of Allium austroiranicum by Cytotoxic Effects against Ovary and Cervical Cancer Cell Lines","authors":"M. S. Dinani, Narges Zakeri Tehrani, F. Shafiee","doi":"10.22127/RJP.2019.190642.1506","DOIUrl":"https://doi.org/10.22127/RJP.2019.190642.1506","url":null,"abstract":"Background and objectives: Cancer is a major health problem in the world. The aim of this study was to extract the flowers of Allium austroiranicum used by Iranian people as a condiment or for its medicinal effects followed by bioassay guided fractionation of the extracts and fractions, using anti-proliferative effects against ovarian and cervical cancer cells. Methods: The air-dried flowers of Allium austroiranicum were extracted in a four-step extraction method, resulting hexan, chloroform, chloroform: methanol (9: 1), butanol and aqueous extracts. Anti-proliferative effects of the extracts were evaluated by MTT assay against OVCAR-3, HeLa, and HUVEC cell lines. The most potent cytotoxic extract was then subjected to fractionation by MPLC method on a RP-18 silicagel column. Finally, the cytotoxic effects of resulted fractions were analyzed again and the most potent cytotoxic fraction and its IC50 were determined. Results: Statistical analysis showed thatbutanol extract of A. austroiranicum showed the most potent cytotoxic effects against OVCAR-3, HeLa and HUVEC cell lines with IC50 values of 38±2, 56±1.4, and 60±3.5 µg/mL, respectively. On the other hand, for 7 fractions resulting from fractionation of the butanol extract, MTT assay results showed that 6th fraction (F) was the most cytotoxic fraction with IC50 of 2.7±0.26 and 7.5±0.5 µg/mL for OVCAR-3 and HeLa cancer cell lines, respectively. Primary evaluation of the fraction by TLC and NMR analysis suggested the steroidal saponins as the main constituents. Conclusion: Allium austroiranicum showed significant cytotoxic effects against ovarian cancer cell line especiallyfractions assumed to contain steroidal saponins. The fraction constituents have the potential of being strong cytotoxic agents and the isolation and identification of compounds are suggested.","PeriodicalId":21088,"journal":{"name":"Research Journal of Pharmacognosy","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68212380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.22127/RJP.2019.206080.1529
Mahdieh Kalkhorani, A. Hadjiakhoondi, N. Yassa, M. Amin, Seyedeh Bahareh Damankash, F. Moradkhani, M. Vazirian
Background and objectives: Centaurea bruguierana subsp. belangeriana (DC.) Bornm. is an annual herb widely distributed in the world. It is used in folk medicine of Iran as an anti-ulcer for gastrointestinal problems. Previous studies have revealed the anti-ulcer effects of the plant extract and in the present study the anti-Helicobacter pylori activity of different fractions, isolated from C. bruguierana subsp. belangeriana was evaluated. Urease and motility inhibition activity were also examined for determination of possible mechanisms. Method: Ethanol 80% was used for the extraction. Chloroform, ethyl acetate, and methanol fractions of the total extract were obtained by solid-liquid extraction. All extracts were evaluated against jack bean urease, bacterial swarming and Minimal Inhibitory Concentrations (MICs) were determined using the agar diffusion method. Bio-guided fractionation was performed by isolation and purification of compounds from active fractions using silica-gel open column chromatography by column and thin layer chromatographic methods and identification by spectroscopic data. Results: Total extract and chloroform fraction possessed the highest anti-H. pylori activity with MIC 325 300 µg/mL and, respectively. The total extract was the most potent urease inhibitor (IC50 250 µg/mL). The motility test confirmed the results by inhibiting swarming at concentrations comparable with IC50. The separation and purification of effective compounds of the chloroform extract was performed according to the results; Lupeol (1), Retusin (2), and Apigenin (3) were isolated and identified. Conclusion: Our research demonstrated a noticeable anti-H.pylori activity of different fractions fromC.bruguierana subsp. belangeriana especially the nonpolar fraction.
{"title":"Bio-guided Fractionation of Centaurea bruguierana subsp. belangeriana Extract Based on Anti-Helicobacter pylori Activity","authors":"Mahdieh Kalkhorani, A. Hadjiakhoondi, N. Yassa, M. Amin, Seyedeh Bahareh Damankash, F. Moradkhani, M. Vazirian","doi":"10.22127/RJP.2019.206080.1529","DOIUrl":"https://doi.org/10.22127/RJP.2019.206080.1529","url":null,"abstract":"Background and objectives: Centaurea bruguierana subsp. belangeriana (DC.) Bornm. is an annual herb widely distributed in the world. It is used in folk medicine of Iran as an anti-ulcer for gastrointestinal problems. Previous studies have revealed the anti-ulcer effects of the plant extract and in the present study the anti-Helicobacter pylori activity of different fractions, isolated from C. bruguierana subsp. belangeriana was evaluated. Urease and motility inhibition activity were also examined for determination of possible mechanisms. Method: Ethanol 80% was used for the extraction. Chloroform, ethyl acetate, and methanol fractions of the total extract were obtained by solid-liquid extraction. All extracts were evaluated against jack bean urease, bacterial swarming and Minimal Inhibitory Concentrations (MICs) were determined using the agar diffusion method. Bio-guided fractionation was performed by isolation and purification of compounds from active fractions using silica-gel open column chromatography by column and thin layer chromatographic methods and identification by spectroscopic data. Results: Total extract and chloroform fraction possessed the highest anti-H. pylori activity with MIC 325 300 µg/mL and, respectively. The total extract was the most potent urease inhibitor (IC50 250 µg/mL). The motility test confirmed the results by inhibiting swarming at concentrations comparable with IC50. The separation and purification of effective compounds of the chloroform extract was performed according to the results; Lupeol (1), Retusin (2), and Apigenin (3) were isolated and identified. Conclusion: Our research demonstrated a noticeable anti-H.pylori activity of different fractions fromC.bruguierana subsp. belangeriana especially the nonpolar fraction.","PeriodicalId":21088,"journal":{"name":"Research Journal of Pharmacognosy","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68212571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.22127/RJP.2018.121067.1372
M. Zangeneh, G. Mohammadi, Saman Salmani, P. R. Tehrani, Khodabakhsh Rashidi, Akram Zangeneh
Background and objectives:Diabetes is the most common metabolic disorder with severe effects on quality of life. Decreasing serum glucose levels and normalization of kidney parameters is of great clinical importance for treating diabetes. Urtica dioica L. has been used in as anti-inflammatory, antioxidant, anti-fungal, and antibacterial agent. To our knowledge, there are little evidences about the anti-diabetic and nephroprotective actions of U. dioica L. The present study was carried out to assess the anti-diabetic and nephroprotective activities of U. dioica aqueous extract (UDAE) in streptozotocin (STZ) induced diabetic mice for 20 days. Methods: Male mice were divided into six groups: normal control, untreated diabetic, diabetic mice receiving 30, 90 and 270 mg/kg of plant extract (groups UDAE30, UDAE90 and UDAE270, respectively) or 30 mg/kg glibenclamide. At 20th day, the mice killed, dissected, then blood and kidney samples were collected for biochemical and histological parameters analysis. Results: Biochemically, UDAE at all doses and glibenclamide could significantly (p≤0.05) reduce the raised levels of blood glucose, urea and creatinine when compared to the untreated group. Histologically, differentdoses of UDAE (especially UDAE270) and glibenclamide could significantly (p≤0.05) decrease the volume and length of the renal structures as compared to the untreated group. Conclusion: These results indicated thatUDAE could improve diabetic related metabolic derangement such as hyperglycemia and elevated kidney markers.
{"title":"A Comparative Evaluation of Nephroprotective Property of Urtica dioica L. Aqueous Extract and Glibenclamide in Diabetic Mice","authors":"M. Zangeneh, G. Mohammadi, Saman Salmani, P. R. Tehrani, Khodabakhsh Rashidi, Akram Zangeneh","doi":"10.22127/RJP.2018.121067.1372","DOIUrl":"https://doi.org/10.22127/RJP.2018.121067.1372","url":null,"abstract":"Background and objectives:Diabetes is the most common metabolic disorder with severe effects on quality of life. Decreasing serum glucose levels and normalization of kidney parameters is of great clinical importance for treating diabetes. Urtica dioica L. has been used in as anti-inflammatory, antioxidant, anti-fungal, and antibacterial agent. To our knowledge, there are little evidences about the anti-diabetic and nephroprotective actions of U. dioica L. The present study was carried out to assess the anti-diabetic and nephroprotective activities of U. dioica aqueous extract (UDAE) in streptozotocin (STZ) induced diabetic mice for 20 days. Methods: Male mice were divided into six groups: normal control, untreated diabetic, diabetic mice receiving 30, 90 and 270 mg/kg of plant extract (groups UDAE30, UDAE90 and UDAE270, respectively) or 30 mg/kg glibenclamide. At 20th day, the mice killed, dissected, then blood and kidney samples were collected for biochemical and histological parameters analysis. Results: Biochemically, UDAE at all doses and glibenclamide could significantly (p≤0.05) reduce the raised levels of blood glucose, urea and creatinine when compared to the untreated group. Histologically, differentdoses of UDAE (especially UDAE270) and glibenclamide could significantly (p≤0.05) decrease the volume and length of the renal structures as compared to the untreated group. Conclusion: These results indicated thatUDAE could improve diabetic related metabolic derangement such as hyperglycemia and elevated kidney markers.","PeriodicalId":21088,"journal":{"name":"Research Journal of Pharmacognosy","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68211825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.22127/RJP.2019.206178.1530
Seyedeh Atieh Naeimi, M. Tansaz, H. Hajimehdipoor, S. Saber
Background and objectives: Oligo-amenorrhea is one of the most common symptoms in poly cystic ovarian syndrome (PCOS) patients and Nigella sativa is a medicinal plant used in Iranian traditional medicine for the treatment of oligo-amenorrhea. The aim of this study was to evaluate the effect of N. sativa oil on oligo-amenorrhea in patients with PCOS. Methods: This study was a double-blinded placebo-controlled clinical trial conducted on 84 PCOS patients with oligo-amenorrhea. Patients were randomly assigned to intervention and placebo group. They used two soft gel capsules of N. sativa oil (500 mg, each capsule) or placebo at night for sixteen weeks. Four indices were used to assess menstruation; the interval between menstruations, duration of menstruation, the occurrence of menstruation and the severity of bleeding. The two groups were compared using analysis of covariance. Result: Fifty five patients completed the study (32 patients in N. sativa group and 23 patients in placebo group). The menstrual interval after the study in the intervention group (45 days, 95% CI) was significantly lower than the control group (86 days). The frequency of menstrual cycle in the intervention group (0.79) was significantly higher than the placebo group (0.48). No serious complication was reported in this clinical trial. Conclusion: findings suggest that N. sativa is an alternative treatment and could be useful for menstrual irregularities in women with PCOS. Further studies are recommended to find the exact mechanisms of N. sativa and its different derivatives.
{"title":"Comparing the Effect of Nigella sativa oil Soft Gel and Placebo on Oligomenorrhea, Amenorrhea and Laboratory Characteristics in Patients with Polycystic Ovarian Syndrome, a Randomized Clinical Trial","authors":"Seyedeh Atieh Naeimi, M. Tansaz, H. Hajimehdipoor, S. Saber","doi":"10.22127/RJP.2019.206178.1530","DOIUrl":"https://doi.org/10.22127/RJP.2019.206178.1530","url":null,"abstract":"Background and objectives: Oligo-amenorrhea is one of the most common symptoms in poly cystic ovarian syndrome (PCOS) patients and Nigella sativa is a medicinal plant used in Iranian traditional medicine for the treatment of oligo-amenorrhea. The aim of this study was to evaluate the effect of N. sativa oil on oligo-amenorrhea in patients with PCOS. Methods: This study was a double-blinded placebo-controlled clinical trial conducted on 84 PCOS patients with oligo-amenorrhea. Patients were randomly assigned to intervention and placebo group. They used two soft gel capsules of N. sativa oil (500 mg, each capsule) or placebo at night for sixteen weeks. Four indices were used to assess menstruation; the interval between menstruations, duration of menstruation, the occurrence of menstruation and the severity of bleeding. The two groups were compared using analysis of covariance. Result: Fifty five patients completed the study (32 patients in N. sativa group and 23 patients in placebo group). The menstrual interval after the study in the intervention group (45 days, 95% CI) was significantly lower than the control group (86 days). The frequency of menstrual cycle in the intervention group (0.79) was significantly higher than the placebo group (0.48). No serious complication was reported in this clinical trial. Conclusion: findings suggest that N. sativa is an alternative treatment and could be useful for menstrual irregularities in women with PCOS. Further studies are recommended to find the exact mechanisms of N. sativa and its different derivatives.","PeriodicalId":21088,"journal":{"name":"Research Journal of Pharmacognosy","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68212612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.22127/RJP.2019.190800.1509
M. Hamidi, Saeed Ghasemi, Bahman Bavafa Bighdilou, D. E. Koohi, Fatemeh Yousefbeyk
Background and objectives: Ecballium elaterium (L.) A. Rich (squirting cucumber) has been used traditionally as a remedy for different disorders such as fever, sinusitis, and rheumatic disease. In the present study, antibacterial, antioxidant, and cytotoxic activities of leaves and fruits were evaluated. Also, total flavonoid and phenolic contents were measured were measured. Methods: The fruits and leaves of E. elaterium were extracted by percolation method with methanol. The antioxidant activity was assessed by 2, 2'-diphenyl-1-picrylhydrazyl (DPPH) methods. Then, the total phenolics and flavonoids contents were measured. The cytotoxicity was tested against three cancerous cell lines (MCF-7, MDA-MB-468 and MKN-45) and a normal cell line (HDF). Antibacterial activity was investigated against Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, and Escherichia coli. Results: The total phenolics content of leaves and fruits were 39.97 and 30.90 mg gallic acid equivalents/g of dry extract, respectively. Also, the total flavonoids content in leaf extract was 49.17 mg quercetin as equivalents/g of dry extract while flavonoids were not detected in the fruit extract. In the DPPH assay, the IC50 values were 1.15 and 1.18 mg/mL for leaves and fruits, respectively.The fruit extract showed the most considerable antibacterial activity (MIC 37.5 mg/mL) against P. aeruginosa. Both extracts indicated cytotoxicity on MDA-MB-468 cells (IC50 264 and 50 µg/mL, respectively). Conclusion: The antioxidant, antibacterial, and cytotoxicity of extracts may be due to some secondary metabolites like phenolic compounds and flavonoids. This study suggests that this plant could be considered for further investigations as a natural source of biological compounds.
{"title":"Evaluation of Antioxidant, Antibacterial and Cytotoxic Activity of Methanol Extract from Leaves and Fruits of Iranian Squirting Cucumber (Ecballium elaterium (L.) A. Rich)","authors":"M. Hamidi, Saeed Ghasemi, Bahman Bavafa Bighdilou, D. E. Koohi, Fatemeh Yousefbeyk","doi":"10.22127/RJP.2019.190800.1509","DOIUrl":"https://doi.org/10.22127/RJP.2019.190800.1509","url":null,"abstract":"Background and objectives: Ecballium elaterium (L.) A. Rich (squirting cucumber) has been used traditionally as a remedy for different disorders such as fever, sinusitis, and rheumatic disease. In the present study, antibacterial, antioxidant, and cytotoxic activities of leaves and fruits were evaluated. Also, total flavonoid and phenolic contents were measured were measured. Methods: The fruits and leaves of E. elaterium were extracted by percolation method with methanol. The antioxidant activity was assessed by 2, 2'-diphenyl-1-picrylhydrazyl (DPPH) methods. Then, the total phenolics and flavonoids contents were measured. The cytotoxicity was tested against three cancerous cell lines (MCF-7, MDA-MB-468 and MKN-45) and a normal cell line (HDF). Antibacterial activity was investigated against Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, and Escherichia coli. Results: The total phenolics content of leaves and fruits were 39.97 and 30.90 mg gallic acid equivalents/g of dry extract, respectively. Also, the total flavonoids content in leaf extract was 49.17 mg quercetin as equivalents/g of dry extract while flavonoids were not detected in the fruit extract. In the DPPH assay, the IC50 values were 1.15 and 1.18 mg/mL for leaves and fruits, respectively.The fruit extract showed the most considerable antibacterial activity (MIC 37.5 mg/mL) against P. aeruginosa. Both extracts indicated cytotoxicity on MDA-MB-468 cells (IC50 264 and 50 µg/mL, respectively). Conclusion: The antioxidant, antibacterial, and cytotoxicity of extracts may be due to some secondary metabolites like phenolic compounds and flavonoids. This study suggests that this plant could be considered for further investigations as a natural source of biological compounds.","PeriodicalId":21088,"journal":{"name":"Research Journal of Pharmacognosy","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68212417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.22127/RJP.2019.185587.1496
M. Sanei, Roshanank Mokaberinejad, F. Roozafzai, Shobeir Rostami Abousaidi
Low-back pain is a common disease and a considerable economic burden in modern societies. Complementary and alternative therapies for assisting management of pain and disability are sought by a large number of patients. In the present work, the most common herbs used to manage Low-back pain in Iranian traditional medicine (Persian medicine) and current literature has been investigated.Chapters about low-back pain (“Vaja-e Zahr”), and radiculopathy (“Erghonnasa”) and sciatica from Liber Continens (Alhavi) and Canon of Medicine were reviewed. All types of plant usage mentioned in the books including oral, rectal, and topical administration, either alone or in combination were recorded. Descriptive statistics (frequency distributions) were used to report the findings. The current literature were searched with “herbal medicine” and “low-back pain” medical subject heading (MeSH) terms in PubMed, EMBASE, ScienceDirect, Scientific Information Database (in Persian), and Google Scholar databases, to investigate medicinal usage of the most suggested plants.Citrullus colocynthis L. Schrad was the most common plant that Rhazesused in compounds and a significant herb used by Avicenna for management of low-back pain and sciatica. Fruit was the main part and rectal route was the main type of administration of colocynth for low-back pain management.In conclusion, we have proposed a new use of Citrullus colocynthis for management of low-back pain and sciatica according to the evidences from Persian medicine. According to current literature survey, no study has yet investigated or suggested the usage of Citrullus colocynthis for low-back pain management. Further laboratory and clinical studies are required to confirm the safety and efficacy of this plant or its components in low-back pain and sciatica management.
腰痛是现代社会中一种常见疾病和相当大的经济负担。辅助和替代疗法，以协助管理疼痛和残疾是由大量的患者寻求。在目前的工作中，研究了伊朗传统医学(波斯医学)和当前文献中用于治疗腰痛的最常见草药。我们回顾了Liber Continens (Alhavi)和Canon of Medicine中关于腰痛(“Vaja-e Zahr”)、神经根病(“Erghonnasa”)和坐骨神经痛的章节。记录了书中提到的所有类型的植物用法，包括口服、直肠和局部给药，无论是单独的还是联合的。描述性统计(频率分布)用于报告研究结果。在PubMed、EMBASE、ScienceDirect、科学信息数据库(波斯语)和谷歌Scholar数据库中使用“草药”和“腰痛”医学主题标题(MeSH)检索当前文献，以调查最受建议的植物的药用用途。香瓜(Citrullus colocynthis L. Schrad)是rhazesis化合物中最常用的植物，也是Avicenna用于治疗腰痛和坐骨神经痛的重要草药。水果是治疗腰痛的主要部位，直肠途径是治疗腰痛的主要给药方式。综上所述，我们根据波斯医学的证据，提出了一种治疗腰痛和坐骨神经痛的新方法。根据目前的文献调查，尚未有研究调查或建议使用香瓜多糖治疗腰痛。需要进一步的实验室和临床研究来证实这种植物或其成分在腰痛和坐骨神经痛治疗中的安全性和有效性。
{"title":"Citrullus colocynthis: the Most Suggested Herb in Persian Medicine for Management of Low-Back Pain","authors":"M. Sanei, Roshanank Mokaberinejad, F. Roozafzai, Shobeir Rostami Abousaidi","doi":"10.22127/RJP.2019.185587.1496","DOIUrl":"https://doi.org/10.22127/RJP.2019.185587.1496","url":null,"abstract":"Low-back pain is a common disease and a considerable economic burden in modern societies. Complementary and alternative therapies for assisting management of pain and disability are sought by a large number of patients. In the present work, the most common herbs used to manage Low-back pain in Iranian traditional medicine (Persian medicine) and current literature has been investigated.Chapters about low-back pain (“Vaja-e Zahr”), and radiculopathy (“Erghonnasa”) and sciatica from Liber Continens (Alhavi) and Canon of Medicine were reviewed. All types of plant usage mentioned in the books including oral, rectal, and topical administration, either alone or in combination were recorded. Descriptive statistics (frequency distributions) were used to report the findings. The current literature were searched with “herbal medicine” and “low-back pain” medical subject heading (MeSH) terms in PubMed, EMBASE, ScienceDirect, Scientific Information Database (in Persian), and Google Scholar databases, to investigate medicinal usage of the most suggested plants.Citrullus colocynthis L. Schrad was the most common plant that Rhazesused in compounds and a significant herb used by Avicenna for management of low-back pain and sciatica. Fruit was the main part and rectal route was the main type of administration of colocynth for low-back pain management.In conclusion, we have proposed a new use of Citrullus colocynthis for management of low-back pain and sciatica according to the evidences from Persian medicine. According to current literature survey, no study has yet investigated or suggested the usage of Citrullus colocynthis for low-back pain management. Further laboratory and clinical studies are required to confirm the safety and efficacy of this plant or its components in low-back pain and sciatica management.","PeriodicalId":21088,"journal":{"name":"Research Journal of Pharmacognosy","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68212261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Asgharian, Masumeh Zadehkamand, A. Delazar, E. Safarzadeh, S. Asnaashari
Background and objectives: The aerial parts of Artemisia marschalliana Sprengel as an indigenous species of genus Artemisia in the East Azerbaijan province of Iran, was subjected to phytochemical analysis, as well asanti-proliferative, free-radical-scavenging and anti-malarialactivities. Methods: The chemical composition of the essential oil obtained from the aerial parts of A. marschalliana was analyzed by GC/MS (gas chromatography/mass spectrometry) and GC/FID (gas chromatography/ flame ionization detector). The anti-proliferative, anti-oxidant, and anti-malarial activities of the essential oil were assessed by MTT, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and cell-free β-hematin formation assays, respectively. Results: A total of 38 constituents were identified, which represented 95.55% of the oil. The essential oil was characterized by a high content of oxygenated sesquiterpenes. The major components of the oil were spathulenol (38.25%), isoaromadendrene epoxide (8.5%), and caryophyllene oxide (7.31%). The oil exhibited cytotoxic activity against the human breast adenocarcinoma (MCF-7) cell line. The half maximal inhibitory concentration (IC50) of anti-malarial assay was 0.38±0.04 mg/mL; the oil, however, displayed low anti-oxidant activity. Conclusion: These findings will be beneficial for the further development of new chemotherapeutic or anti-malarial agents.
{"title":"Chemical Composition and Some Biological Activities of Artemisia marschalliana Essential Oil","authors":"P. Asgharian, Masumeh Zadehkamand, A. Delazar, E. Safarzadeh, S. Asnaashari","doi":"10.22127/RJP.2019.93527","DOIUrl":"https://doi.org/10.22127/RJP.2019.93527","url":null,"abstract":"Background and objectives: The aerial parts of Artemisia marschalliana Sprengel as an indigenous species of genus Artemisia in the East Azerbaijan province of Iran, was subjected to phytochemical analysis, as well asanti-proliferative, free-radical-scavenging and anti-malarialactivities. Methods: The chemical composition of the essential oil obtained from the aerial parts of A. marschalliana was analyzed by GC/MS (gas chromatography/mass spectrometry) and GC/FID (gas chromatography/ flame ionization detector). The anti-proliferative, anti-oxidant, and anti-malarial activities of the essential oil were assessed by MTT, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and cell-free β-hematin formation assays, respectively. Results: A total of 38 constituents were identified, which represented 95.55% of the oil. The essential oil was characterized by a high content of oxygenated sesquiterpenes. The major components of the oil were spathulenol (38.25%), isoaromadendrene epoxide (8.5%), and caryophyllene oxide (7.31%). The oil exhibited cytotoxic activity against the human breast adenocarcinoma (MCF-7) cell line. The half maximal inhibitory concentration (IC50) of anti-malarial assay was 0.38±0.04 mg/mL; the oil, however, displayed low anti-oxidant activity. Conclusion: These findings will be beneficial for the further development of new chemotherapeutic or anti-malarial agents.","PeriodicalId":21088,"journal":{"name":"Research Journal of Pharmacognosy","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48519790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Rezaei-Tavirani, M. Tavirani, M. Azodi, Z. Akbari, H. Hajimehdipoor
Background and objectives: Non-alcoholic fatty liver disease (NAFLD) is a common liver condition. On the other hand, coffee consumption has shown promising for gastrointestinal diseases. Detection of the most valuable biomarkers of decaffeinated coffee treatment in healthy and non-alcoholic fatty liver disease conditions was the aim of the present study. Methods: A previous proteomics study about effect of decaffeinated coffee (1.5 mL daily drinking coffee for two months) on protein expression change of rat liver was selected for protein-protein interaction (PPI) network analysis via Cytoscape v.3.7.1 and the related applications. The most central proteins with regards to a high degree and betweenness centralities in the coffee treatment condition of healthy and NAFLD were then analyzed by ClueGO for biological process (BP) derivation. Results: HSPA5, HSPA4, HSPA9, HSPA7, PARK7, HSP90AA1, P4HB, PRDX1, and PDIA3 were introduced as central proteins, which are involved in folding and antioxidant activities. Conclusion: There is a complicated combination of the components in coffee; some elements are involved in liver protection against NAFLD and the others are in contrast.
{"title":"Prediction of Coffee Effects in Rats with Healthy and NAFLD Conditions Based on Protein-Protein Interaction Network Analysis","authors":"M. Rezaei-Tavirani, M. Tavirani, M. Azodi, Z. Akbari, H. Hajimehdipoor","doi":"10.22127/RJP.2019.93500","DOIUrl":"https://doi.org/10.22127/RJP.2019.93500","url":null,"abstract":"Background and objectives: Non-alcoholic fatty liver disease (NAFLD) is a common liver condition. On the other hand, coffee consumption has shown promising for gastrointestinal diseases. Detection of the most valuable biomarkers of decaffeinated coffee treatment in healthy and non-alcoholic fatty liver disease conditions was the aim of the present study. Methods: A previous proteomics study about effect of decaffeinated coffee (1.5 mL daily drinking coffee for two months) on protein expression change of rat liver was selected for protein-protein interaction (PPI) network analysis via Cytoscape v.3.7.1 and the related applications. The most central proteins with regards to a high degree and betweenness centralities in the coffee treatment condition of healthy and NAFLD were then analyzed by ClueGO for biological process (BP) derivation. Results: HSPA5, HSPA4, HSPA9, HSPA7, PARK7, HSP90AA1, P4HB, PRDX1, and PDIA3 were introduced as central proteins, which are involved in folding and antioxidant activities. Conclusion: There is a complicated combination of the components in coffee; some elements are involved in liver protection against NAFLD and the others are in contrast.","PeriodicalId":21088,"journal":{"name":"Research Journal of Pharmacognosy","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46838149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Zangeneh, Akram Zangeneh, R. Tahvilian, R. Moradi
Background and objectives: Tragopogon graminifolius DC. is widely used in Iranian traditional medicine as a gastro-protective and wound healer agent. In the present study the hepatoprotective property of T. graminifolius DC. aqueous extract (TGAE) was investigated against carbon tetrachloride (CCl4) induced hepatocellular injury in mice. Methods: Fifty male mice were divided into five groups (n=10). Group 1 (control) received 1 ml/kg olive oil intraperitoneally (i.p.) and distilled water orally; Group 2 (untreated) received CCl4 (50% in olive oil, 1 ml/kg; i.p.); Groups 3, 4 and 5 received CCl4 and 30, 90 and 270 mg/kg of TGAE (TGAE30, TGAE90 and TGAE270), respectively. At the end of the 45-day treatment, the mice of all groups were euthanized, then blood and liver samples were collected for biochemical and histological parameters analysis. The data was analyzed by one way variance analysis and Duncan’s test using SPSS 21. Results: Different doses of TGAE (especially TGAE270) could significantly (p≤0.05) reduce the raised levels of ALP, AST, ALT, Cholesterol, LDL and increased HDL, SOD, CAT as compared to the untreated group. The weight and volume of the hepatic structures decreased significantly (p≤0.05) in several doses of TGAE (especially TGAE270) when compared to the untreated group. Conclusion: The results of the present study showed that under the present experimental conditions, TGAE indicated hepatoprotective abilities against CCl4 induced liver damage in mice.
{"title":"Assessment of the Hepatoprotective Activity of Tragopogon graminifolius DC. Aqueous Extract on CCl4- Induced Hepatotoxicity in Mice","authors":"M. Zangeneh, Akram Zangeneh, R. Tahvilian, R. Moradi","doi":"10.22127/RJP.2019.93525","DOIUrl":"https://doi.org/10.22127/RJP.2019.93525","url":null,"abstract":"Background and objectives: Tragopogon graminifolius DC. is widely used in Iranian traditional medicine as a gastro-protective and wound healer agent. In the present study the hepatoprotective property of T. graminifolius DC. aqueous extract (TGAE) was investigated against carbon tetrachloride (CCl4) induced hepatocellular injury in mice. Methods: Fifty male mice were divided into five groups (n=10). Group 1 (control) received 1 ml/kg olive oil intraperitoneally (i.p.) and distilled water orally; Group 2 (untreated) received CCl4 (50% in olive oil, 1 ml/kg; i.p.); Groups 3, 4 and 5 received CCl4 and 30, 90 and 270 mg/kg of TGAE (TGAE30, TGAE90 and TGAE270), respectively. At the end of the 45-day treatment, the mice of all groups were euthanized, then blood and liver samples were collected for biochemical and histological parameters analysis. The data was analyzed by one way variance analysis and Duncan’s test using SPSS 21. Results: Different doses of TGAE (especially TGAE270) could significantly (p≤0.05) reduce the raised levels of ALP, AST, ALT, Cholesterol, LDL and increased HDL, SOD, CAT as compared to the untreated group. The weight and volume of the hepatic structures decreased significantly (p≤0.05) in several doses of TGAE (especially TGAE270) when compared to the untreated group. Conclusion: The results of the present study showed that under the present experimental conditions, TGAE indicated hepatoprotective abilities against CCl4 induced liver damage in mice.","PeriodicalId":21088,"journal":{"name":"Research Journal of Pharmacognosy","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47149443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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