Pub Date : 2025-04-01DOI: 10.1016/j.ram.2024.10.005
Nicolás Cimmino , Maria Josefina Etchevers , Astrid Smud , Patricia Yolleth Andrade Pino , Marisa del Lujan Sanchez , Maria Laura Gonzalez , Maria Victoria Acosta , Gissel Zuñiga , Karina Alejandra Perez , Juana Freggiaro , Valeria Rosa Alexander , Maria Florencia Veiga , Mariangeles Visus , Mariano Martin Marcolongo
Infectious diarrhea is a common health issue that affects a large number of individuals each year. It causes significant morbidity and mortality, greatly impacting healthcare system costs. Rapid detection of the causative organism and timely treatment alters the management and outcome of the condition. Molecular panels in stool allow to analyze a wide range of pathogens quickly and easily. For this study, a cross-sectional cohort analysis with a retrospective analysis of adult patients hospitalized with diarrhea and negative conventional stool bacteriological studies was conducted. Data obtained from the use of molecular panels in stool and the role of endoscopy in the diagnostic pathway were analyzed. A positivity rate of 52% (n = 41) out of a total of 79 samples was reported. The test contributed to a change in therapeutic approach in 58% (n = 46) of the patients. Among the patients with a negative molecular panel, 39.5% underwent further evaluation with colonoscopy involving biopsies, resulting in a diagnostic yield of 87%. Based on these results, we can conclude that molecular techniques contribute to the diagnosis and change in therapeutic approach in hospitalized patients with diarrhea.
{"title":"Use of stool molecular panel in hospitalized patients with diarrhea. Experience in a tertiary care center","authors":"Nicolás Cimmino , Maria Josefina Etchevers , Astrid Smud , Patricia Yolleth Andrade Pino , Marisa del Lujan Sanchez , Maria Laura Gonzalez , Maria Victoria Acosta , Gissel Zuñiga , Karina Alejandra Perez , Juana Freggiaro , Valeria Rosa Alexander , Maria Florencia Veiga , Mariangeles Visus , Mariano Martin Marcolongo","doi":"10.1016/j.ram.2024.10.005","DOIUrl":"10.1016/j.ram.2024.10.005","url":null,"abstract":"<div><div>Infectious diarrhea is a common health issue that affects a large number of individuals each year. It causes significant morbidity and mortality, greatly impacting healthcare system costs. Rapid detection of the causative organism and timely treatment alters the management and outcome of the condition. Molecular panels in stool allow to analyze a wide range of pathogens quickly and easily. For this study, a cross-sectional cohort analysis with a retrospective analysis of adult patients hospitalized with diarrhea and negative conventional stool bacteriological studies was conducted. Data obtained from the use of molecular panels in stool and the role of endoscopy in the diagnostic pathway were analyzed. A positivity rate of 52% (n<!--> <!-->=<!--> <!-->41) out of a total of 79 samples was reported. The test contributed to a change in therapeutic approach in 58% (n<!--> <!-->=<!--> <!-->46) of the patients. Among the patients with a negative molecular panel, 39.5% underwent further evaluation with colonoscopy involving biopsies, resulting in a diagnostic yield of 87%. Based on these results, we can conclude that molecular techniques contribute to the diagnosis and change in therapeutic approach in hospitalized patients with diarrhea.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 98-104"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-01DOI: 10.1016/j.ram.2024.11.003
Raquel Jara , Dafne Santos , Noelia Soledad Reyes , Eliana Hermida , Alejandro Seoane , Martin Ypas , Gisela Andres , Marcela Echavarría
Acute respiratory infection (ARI) is one of the principal causes of morbidity worldwide, with respiratory viruses being common etiological agents. Among them, endemic human coronaviruses (hCoVs) including CoV-229E, CoV-OC43, CoV-NL63, and CoV-HKU1 can cause mild ARI but are usually not evaluated in the clinical setting. The aim of this work was to determine the prevalence of all respiratory pathogens, with the focus placed on endemic hCoVs in the pre-pandemic period. Circulating species, clinical associations and coinfections with other respiratory pathogens were evaluated in 510 immunocompetent patients (children and adults) with ARI using the FilmArray® Respiratory Panel (BioFire/bioMérieux). A total of 399 children (252 outpatients and 147 hospitalized) and 111 adult outpatients were enrolled in the pre-pandemic period (2008–2010 and 2016). Endemic hCoVs were the third and fifth more frequently detected viruses among adults and outpatient children, respectively, with an overall frequency close to 10%. The most prevalent species were CoV-OC43 (42.8%) and CoV-HKU1 (40.5%), followed by CoV-NL63 (19.0%) and CoV-229E (4.8%). Tachypnea, wheezing and chest indrawing were more frequent in hospitalized children compared to outpatients. All adult patients presented with symptoms of a common cold. Endemic hCoVs were detected year-round, primarily between June and November. Our results highlight their clinical relevance, and the need to include endemic hCoVs in routine screening. In the post-pandemic period, further long-term surveillance is needed for understanding the epidemiology of endemic hCoVs and their evolution, as a tool to anticipate the possible emergence of new species.
{"title":"Endemic coronavirus in children and adults with acute respiratory infection before the COVID-19 pandemic","authors":"Raquel Jara , Dafne Santos , Noelia Soledad Reyes , Eliana Hermida , Alejandro Seoane , Martin Ypas , Gisela Andres , Marcela Echavarría","doi":"10.1016/j.ram.2024.11.003","DOIUrl":"10.1016/j.ram.2024.11.003","url":null,"abstract":"<div><div>Acute respiratory infection (ARI) is one of the principal causes of morbidity worldwide, with respiratory viruses being common etiological agents. Among them, endemic human coronaviruses (hCoVs) including CoV-229E, CoV-OC43, CoV-NL63, and CoV-HKU1 can cause mild ARI but are usually not evaluated in the clinical setting. The aim of this work was to determine the prevalence of all respiratory pathogens, with the focus placed on endemic hCoVs in the pre-pandemic period. Circulating species, clinical associations and coinfections with other respiratory pathogens were evaluated in 510 immunocompetent patients (children and adults) with ARI using the FilmArray® Respiratory Panel (BioFire/bioMérieux). A total of 399 children (252 outpatients and 147 hospitalized) and 111 adult outpatients were enrolled in the pre-pandemic period (2008–2010 and 2016). Endemic hCoVs were the third and fifth more frequently detected viruses among adults and outpatient children, respectively, with an overall frequency close to 10%. The most prevalent species were CoV-OC43 (42.8%) and CoV-HKU1 (40.5%), followed by CoV-NL63 (19.0%) and CoV-229E (4.8%). Tachypnea, wheezing and chest indrawing were more frequent in hospitalized children compared to outpatients. All adult patients presented with symptoms of a common cold. Endemic hCoVs were detected year-round, primarily between June and November. Our results highlight their clinical relevance, and the need to include endemic hCoVs in routine screening. In the post-pandemic period, further long-term surveillance is needed for understanding the epidemiology of endemic hCoVs and their evolution, as a tool to anticipate the possible emergence of new species.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 161-170"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-01DOI: 10.1016/j.ram.2024.11.001
Eduardo Montalvo , Florencia Veiga , Hernán Rodríguez , German Traglia , Carlos Vay , Marisa Almuzara
Aeromonas spp. are opportunistic pathogens that cause both intra- and extraintestinal infections. The objective of this work was the phenotypic and genotypic characterization of a collection of Aeromonas strains, in addition to determining their sensitivity to different antimicrobials. Thirty seven isolates were analyzed. 54% were of intra-abdominal origin, 22% from skin and soft tissues, 19% from the bloodstream, among other less frequent sites. By amplification and sequencing of the gyrB gene, which was considered the reference method, the following were identified: 37,8% as species of the Aeromonas hydrophila complex, 32,4% as species of the Aeromonas veronii complex, and 29,7% as species of the complex Aeromonas caviae. Identification by traditional biochemical tests presented a better correlation with molecular identification than mass spectrometry (MALDI TOF MS). Regarding antibiotic sensitivity, cefotaxime, ceftazidime, cefepime, piperacillin-tazobactam, trimethoprim-sulfamethoxazole, ciprofloxacin, amikacin, gentamicin and nitrofurantoin showed activity on more than 80.0% of the isolates tested. The sensitivity and specificity of the phenotypic methods to determine the presence of carbapenemases in relation to the detection of the cphAgene, the reference method, was 60,9% and 100%, respectively, for the colorimetric assay (Blue Carba), and of 91,3% and 50,0% respectively, for the modified Hodge test. The overall resistance to colistin was 32,4%. The automated method showed a very higher error (VME) of 16,2%, while the rapid colorimetric screening method (CRTc) showed an excellent correlation (VME 0%) with the reference method, broth microdilution.
{"title":"Identificación y sensibilidad antibiótica de aislamientos de Aeromonas spp. en un Hospital Universitario de la ciudad de Buenos Aires","authors":"Eduardo Montalvo , Florencia Veiga , Hernán Rodríguez , German Traglia , Carlos Vay , Marisa Almuzara","doi":"10.1016/j.ram.2024.11.001","DOIUrl":"10.1016/j.ram.2024.11.001","url":null,"abstract":"<div><div><em>Aeromonas</em> spp. are opportunistic pathogens that cause both intra- and extraintestinal infections. The objective of this work was the phenotypic and genotypic characterization of a collection of <em>Aeromonas</em> strains, in addition to determining their sensitivity to different antimicrobials. Thirty seven isolates were analyzed. 54% were of intra-abdominal origin, 22% from skin and soft tissues, 19% from the bloodstream, among other less frequent sites. By amplification and sequencing of the <em>gyrB</em> gene, which was considered the reference method, the following were identified: 37,8% as species of the <em>Aeromonas hydrophila</em> complex, 32,4% as species of the <em>Aeromonas veronii</em> complex, and 29,7% as species of the complex <em>Aeromonas caviae</em>. Identification by traditional biochemical tests presented a better correlation with molecular identification than mass spectrometry (MALDI TOF MS). Regarding antibiotic sensitivity, cefotaxime, ceftazidime, cefepime, piperacillin-tazobactam, trimethoprim-sulfamethoxazole, ciprofloxacin, amikacin, gentamicin and nitrofurantoin showed activity on more than 80.0% of the isolates tested. The sensitivity and specificity of the phenotypic methods to determine the presence of carbapenemases in relation to the detection of the cphAgene, the reference method, was 60,9% and 100%, respectively, for the colorimetric assay (Blue Carba), and of 91,3% and 50,0% respectively, for the modified Hodge test. The overall resistance to colistin was 32,4%. The automated method showed a very higher error (VME) of 16,2%, while the rapid colorimetric screening method (CRTc) showed an excellent correlation (VME 0%) with the reference method, broth microdilution.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 2","pages":"Pages 105-113"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.ram.2024.07.002
César Ernesto Gutiérrez , María Agustina Jaras Horno , Carolina Aro , Andrea Florencia Gómez Colussi , María Belén Cheirano , Érica Soledad Rodríguez , María Verónica Vera Garate , Guillermo García Effron , Sergio Adrián Guerrero
Intestinal parasitic diseases constitute a cosmopolitan public health concern, with greater prevalence in developing countries, and mainly affecting children. The aim of this study was to develop an educational intervention aimed at mothers/guardians of children attending three child development centers (CDI) in Santa Fe, Argentina, during 2018. An educational intervention was conducted using a descriptive, quasi-experimental design, with pre- and post-intervention assessment. This intervention included 36 mothers, and was carried out in three stages: diagnosis, intervention and final evaluation. Simultaneously, a coproparasitological study was performed on 48 children under 5 years of age, who were assisted in the CDI, with the aim to understand and address the parasitic infections prevalent in the child population. It was possible to compare the correct answers before and after the educational intervention, observing a statistically significant increase (p = 0.008742) in the average number of correct answers. Enteroparasites were identified in 54% of the population of children analyzed, 10% were biparasitized and 6% had more than two parasitic species. The most frequently detected parasites were Blastocystis spp., Giardia intestinalis and Ascaris lumbricoides. The educational intervention was positive, resulting in an improvement in the level of knowledge related to intestinal parasitosis and its prevention. This educational intervention experience highlights the importance of ongoing education in promoting a healthy lifestyle and preventing parasitosis in vulnerable populations.
{"title":"Intervención educativa como estrategia de prevención de parasitosis intestinales y valoración del estado enteroparasitológico en centros de desarrollo infantil de la ciudad de Santa Fe, Argentina","authors":"César Ernesto Gutiérrez , María Agustina Jaras Horno , Carolina Aro , Andrea Florencia Gómez Colussi , María Belén Cheirano , Érica Soledad Rodríguez , María Verónica Vera Garate , Guillermo García Effron , Sergio Adrián Guerrero","doi":"10.1016/j.ram.2024.07.002","DOIUrl":"10.1016/j.ram.2024.07.002","url":null,"abstract":"<div><div>Intestinal parasitic diseases constitute a cosmopolitan public health concern, with greater prevalence in developing countries, and mainly affecting children. The aim of this study was to develop an educational intervention aimed at mothers/guardians of children attending three child development centers (CDI) in Santa Fe, Argentina, during 2018. An educational intervention was conducted using a descriptive, quasi-experimental design, with pre- and post-intervention assessment. This intervention included 36 mothers, and was carried out in three stages: diagnosis, intervention and final evaluation. Simultaneously, a coproparasitological study was performed on 48 children under 5 years of age, who were assisted in the CDI, with the aim to understand and address the parasitic infections prevalent in the child population. It was possible to compare the correct answers before and after the educational intervention, observing a statistically significant increase (<em>p</em> <!-->=<!--> <!-->0.008742) in the average number of correct answers. Enteroparasites were identified in 54% of the population of children analyzed, 10% were biparasitized and 6% had more than two parasitic species. The most frequently detected parasites were <em>Blastocystis</em> spp., <em>Giardia intestinalis</em> and <em>Ascaris lumbricoides</em>. The educational intervention was positive, resulting in an improvement in the level of knowledge related to intestinal parasitosis and its prevention. This educational intervention experience highlights the importance of ongoing education in promoting a healthy lifestyle and preventing parasitosis in vulnerable populations.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 1","pages":"Pages 8-13"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142126527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.ram.2024.10.004
Elena Berardinelli , Marcela Sokol , Laura Dufranc , Vanina Diaz , Viviana Eiras , Ariel Gianecini , Patricia Galarza , Paula Gagetti , Federico Lorenzo , Adriana Efron
Bartholinitis is the inflammation and infection of the Bartholin's glands that results from the accumulation of mucus in their ducts, the most frequent causal microorganisms being anaerobic and aerobic bacteria and those responsible for sexually transmitted infections. Those caused by agents not belonging to the genital microbiota are less frequent. Likewise, in most cases the diagnosis is clinical. Here, we describe the case of a 42-year-old heterosexual woman with a Bartholin's gland abscess caused by Neisseria meningitidis, isolated through culture and identified by VITEK®2. For molecular characterization and subtyping, the sequence type and clonal complex were determined using the multiple locus sequence typing technique. The antigenic profile was studied by amplification and sequencing of the genes coding for PorA, fHbp, NHBA and NadA and the susceptibility profile was assessed by MIC. Molecular diagnosis led to the confirmation of N. meningitidis as the pathogen responsible for bartholinitis.
{"title":"Bartolinitis por Neisseria meningitidis: caso clínico","authors":"Elena Berardinelli , Marcela Sokol , Laura Dufranc , Vanina Diaz , Viviana Eiras , Ariel Gianecini , Patricia Galarza , Paula Gagetti , Federico Lorenzo , Adriana Efron","doi":"10.1016/j.ram.2024.10.004","DOIUrl":"10.1016/j.ram.2024.10.004","url":null,"abstract":"<div><div>Bartholinitis is the inflammation and infection of the Bartholin's glands that results from the accumulation of mucus in their ducts, the most frequent causal microorganisms being anaerobic and aerobic bacteria and those responsible for sexually transmitted infections. Those caused by agents not belonging to the genital microbiota are less frequent. Likewise, in most cases the diagnosis is clinical. Here, we describe the case of a 42-year-old heterosexual woman with a Bartholin's gland abscess caused by <em>Neisseria meningitidis</em>, isolated through culture and identified by VITEK®2. For molecular characterization and subtyping, the sequence type and clonal complex were determined using the multiple locus sequence typing technique. The antigenic profile was studied by amplification and sequencing of the genes coding for PorA, fHbp, NHBA and NadA and the susceptibility profile was assessed by MIC. Molecular diagnosis led to the confirmation of <em>N. meningitidis</em> as the pathogen responsible for bartholinitis.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 1","pages":"Pages 63-65"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.ram.2024.10.001
Ronak Bakhtiari , Maliheh Shiri , Mohammad Reza Mohammadi , Mohammad Reza Pourmand , Amir Mirzaie , Zahra Taghiabadi
Methicillin-resistant Staphylococcus aureus (MRSA) causes a wide range of infections and contributes to elevated morbidity, mortality, and healthcare costs. Herbal compounds combined with drug delivery systems could be an effective alternative option for treating resistant bacteria. This study evaluates the antimicrobial prowess of carvacrol-loaded niosomes against MRSA strains. In this study, six carvacrol–niosome formulations with different ratios of Span and Tween were prepared. The physicochemical attributes of the optimized synthesized niosomes were assessed using Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) as well as DLS Zetasizer. Encapsulation efficiency (EE) and in vitro drug release were studied. The antibacterial activity of the synthesized carvacrol–niosomes, in concentrations varying between 7.8 and 1000 μg/ml, was evaluated using microdilution broth methods. The optimized niosomes, with a size of 207.3 nm and an impressive EE of 91%, exhibited a spherical structure as confirmed by the electron microscopy analysis. Impressively, these carvacrol–niosomes demonstrated superior antimicrobial effectiveness against S. aureus, reducing MIC levels 4-fold to 62.5 ± 0.0 μg/ml and MBC to 125 ± 0.0 μg/ml, a significant improvement over the 250 ± 0.0 μg/ml MIC and 500 ± 0.0 μg/ml MBC of free carvacrol. Additionally, while empty niosomes showed minimal cytotoxicity with 88.32 ± 1.32% cell viability at 100 μg/ml, free carvacrol led to a marked reduction in viability to 39.46 ± 1.26%. However, niosomes encapsulating carvacrol notably increased cell survival to 59.67 ± 1.62% at this concentration. These findings underscore the enhanced antimicrobial potency of carvacrol when enclosed within niosomes, suggesting its potential as a potent herbal remedy for combating methicillin-resistant S. aureus.
{"title":"Enhanced antimicrobial effects of carvacrol against methicillin-resistant Staphylococcus aureus strains using niosome formulations","authors":"Ronak Bakhtiari , Maliheh Shiri , Mohammad Reza Mohammadi , Mohammad Reza Pourmand , Amir Mirzaie , Zahra Taghiabadi","doi":"10.1016/j.ram.2024.10.001","DOIUrl":"10.1016/j.ram.2024.10.001","url":null,"abstract":"<div><div>Methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) causes a wide range of infections and contributes to elevated morbidity, mortality, and healthcare costs. Herbal compounds combined with drug delivery systems could be an effective alternative option for treating resistant bacteria. This study evaluates the antimicrobial prowess of carvacrol-loaded niosomes against MRSA strains. In this study, six carvacrol–niosome formulations with different ratios of Span and Tween were prepared. The physicochemical attributes of the optimized synthesized niosomes were assessed using Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) as well as DLS Zetasizer. Encapsulation efficiency (EE) and <em>in vitro</em> drug release were studied. The antibacterial activity of the synthesized carvacrol–niosomes, in concentrations varying between 7.8 and 1000<!--> <!-->μg/ml, was evaluated using microdilution broth methods. The optimized niosomes, with a size of 207.3<!--> <!-->nm and an impressive EE of 91%, exhibited a spherical structure as confirmed by the electron microscopy analysis. Impressively, these carvacrol–niosomes demonstrated superior antimicrobial effectiveness against <em>S. aureus</em>, reducing MIC levels 4-fold to 62.5<!--> <!-->±<!--> <!-->0.0<!--> <!-->μg/ml and MBC to 125<!--> <!-->±<!--> <!-->0.0<!--> <!-->μg/ml, a significant improvement over the 250<!--> <!-->±<!--> <!-->0.0<!--> <!-->μg/ml MIC and 500<!--> <!-->±<!--> <!-->0.0<!--> <!-->μg/ml MBC of free carvacrol. Additionally, while empty niosomes showed minimal cytotoxicity with 88.32<!--> <!-->±<!--> <!-->1.32% cell viability at 100<!--> <!-->μg/ml, free carvacrol led to a marked reduction in viability to 39.46<!--> <!-->±<!--> <!-->1.26%. However, niosomes encapsulating carvacrol notably increased cell survival to 59.67<!--> <!-->±<!--> <!-->1.62% at this concentration. These findings underscore the enhanced antimicrobial potency of carvacrol when enclosed within niosomes, suggesting its potential as a potent herbal remedy for combating methicillin-resistant <em>S. aureus</em>.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 1","pages":"Pages 39-48"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.ram.2024.12.002
Nube María Tenezaca Lliguin , Paola Patricia Orellana Bravo , Carlos Fernando Andrade Tacuri , Jonnathan Gerardo Ortiz Tejedor
Staphylococcus aureus is an important pathogen in healthcare facilities, with its resistance to a number of antibiotics currently being a global concern. In this report the presence of S. aureus, resistance gene virulence and antibiotic susceptibility profiles were determined in the mobile phones of senior nursing students. S. aureus was isolated in 11.84% (9/76) of the samples. Furthermore, 44.44% of the mobile phones carried the mecA (MRSA) gene, while none carried the vanA gene. Virulence genes identified were 100% hla, 88.89% hlb, 22.22% tst and sec, and 11.11% sea. The antibiogram revealed that 33.33% of the strains were resistant to cefoxitin and 44.44% showed inducible resistance to clindamycin (ICRSA). The mobile phones of senior nursing students represent an important reservoir of drug-resistant and virulent strains of S. aureus, which could act as infectious foci for the transmission of this pathogen.
{"title":"Staphylococcus aureus resistentes a meticilina aislados de teléfonos móviles de estudiantes de Enfermería en Cuenca, Ecuador","authors":"Nube María Tenezaca Lliguin , Paola Patricia Orellana Bravo , Carlos Fernando Andrade Tacuri , Jonnathan Gerardo Ortiz Tejedor","doi":"10.1016/j.ram.2024.12.002","DOIUrl":"10.1016/j.ram.2024.12.002","url":null,"abstract":"<div><div><em>Staphylococcus aureus</em> is an important pathogen in healthcare facilities, with its resistance to a number of antibiotics currently being a global concern. In this report the presence of <em>S.<!--> <!-->aureus</em>, resistance gene virulence and antibiotic susceptibility profiles were determined in the mobile phones of senior nursing students. <em>S.<!--> <!-->aureus</em> was isolated in 11.84% (9/76) of the samples. Furthermore, 44.44% of the mobile phones carried the <em>mecA</em> (MRSA) gene, while none carried the <em>vanA</em> gene. Virulence genes identified were 100% <em>hla</em>, 88.89% <em>hlb</em>, 22.22% <em>tst</em> and <em>sec</em>, and 11.11% <em>sea</em>. The antibiogram revealed that 33.33% of the strains were resistant to cefoxitin and 44.44% showed inducible resistance to clindamycin (ICRSA). The mobile phones of senior nursing students represent an important reservoir of drug-resistant and virulent strains of <em>S.<!--> <!-->aureus</em>, which could act as infectious foci for the transmission of this pathogen.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 1","pages":"Pages 54-58"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.ram.2025.01.003
Aníbal R. Lodeiro
{"title":"Symbiotic nitrogen-fixing rhizobia as a potential source of nitrous oxide emissions","authors":"Aníbal R. Lodeiro","doi":"10.1016/j.ram.2025.01.003","DOIUrl":"10.1016/j.ram.2025.01.003","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 1","pages":"Pages 1-2"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.ram.2024.09.004
Agustina Costa, Florencia Catalano, Andrea Alcain, Marcela Panagopulo, Jesús Emmanuel Riquel Moyelak, Silvina Brengi, Mirian Moroni, María Rosa Viñas
Salmonella spp. is one of the most important zoonotic pathogens that causes foodborne diseases. It is divided into two species (Salmonella bongori and Salmonella enterica) including around 2600 serovars, being S. enterica serovar Enteritidis one of the most frequent in Argentina. Serovar identification is generally conducted by somatic and flagellar agglutination sera assays, and molecular biology techniques can also be performed. As efforts are being made worldwide to differentiate Salmonella serovars, our aim was to evaluate the utility of two specific biomarkers, previously reported for differentiating S. Enteritidis with MALDI-TOF MS. A panel of 105 S. enterica local isolates, belonging to different serovars and characterized by sera agglutination and PCR, was included in this study. Two specific S. Enteritidis biomarkers, at m/z 3016 ± 3 Da and 6034 ± 3 Da, were visually detected showing a sensitivity of 54% and 98%, respectively, and a specificity of 100% for both peaks. Concordance between serotyping and identification by PCR of S. Enteritidis and the blind search of biomarkers in a subset of isolates was 98%. Visual detection of these S. Enteritidis biomarkers using MALDI-TOF MS can be used as a fast and easy screening method for serovars differentiation at the microbiology clinical laboratory.
{"title":"Rapid discrimination of Salmonella Enteritidis from other serovars with MALDI-TOF MS in Argentina","authors":"Agustina Costa, Florencia Catalano, Andrea Alcain, Marcela Panagopulo, Jesús Emmanuel Riquel Moyelak, Silvina Brengi, Mirian Moroni, María Rosa Viñas","doi":"10.1016/j.ram.2024.09.004","DOIUrl":"10.1016/j.ram.2024.09.004","url":null,"abstract":"<div><div><em>Salmonella</em> spp. is one of the most important zoonotic pathogens that causes foodborne diseases. It is divided into two species (<em>Salmonella bongori</em> and <em>Salmonella enterica</em>) including around 2600 serovars, being <em>S. enterica</em> serovar Enteritidis one of the most frequent in Argentina. Serovar identification is generally conducted by somatic and flagellar agglutination sera assays, and molecular biology techniques can also be performed. As efforts are being made worldwide to differentiate <em>Salmonella</em> serovars, our aim was to evaluate the utility of two specific biomarkers, previously reported for differentiating <em>S.</em> Enteritidis with MALDI-TOF MS. A panel of 105 <em>S. enterica</em> local isolates, belonging to different serovars and characterized by sera agglutination and PCR, was included in this study. Two specific <em>S.</em> Enteritidis biomarkers, at <em>m/z</em> 3016<!--> <!-->±<!--> <!-->3<!--> <!-->Da and 6034<!--> <!-->±<!--> <!-->3<!--> <!-->Da, were visually detected showing a sensitivity of 54% and 98%, respectively, and a specificity of 100% for both peaks. Concordance between serotyping and identification by PCR of <em>S.</em> Enteritidis and the blind search of biomarkers in a subset of isolates was 98%. Visual detection of these <em>S.</em> Enteritidis biomarkers using MALDI-TOF MS can be used as a fast and easy screening method for serovars differentiation at the microbiology clinical laboratory.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 1","pages":"Pages 24-32"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142627162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.ram.2024.10.007
Juan Antonio Castro-Diego , Carlos Alfonso López-Orona , Verónica Delgado-Pacheco , Miguel Armando López-Beltrán , Nancy Ley-López , Walter Arturo Rubio-Aragón , Jorge Alberto Edeza-Urías
Powdery mildew by Podosphaera xanthii (Castagne) is a major disease of greenhouse cucurbitaceous crops worldwide. Honey by honeybees has been reported as an antimicrobial for diseases in humans, animals, and plants. The aim of this study was to assess Apis mellifera honey against P. xanthii in cucumber plants. During nine consecutive weeks, four different honey concentrations (2.0%, 2.5%, 3.0% and 3.5%), a chemical control (Azoxystrobin) and an untreated check (water) were evaluated. Except for honey at 2%, every concentration was significantly different from the untreated check. Honey concentrations at 3% and 3.5% were found to be the most effective, and their area under disease progress curve (AUDP) was statistically comparable to that of Azoxystrobin with 1048.3, 642.3 and 575.8 AUDP, representing 72.4%, 83.1% and 84.8% of efficiency compared to the untreated check, respectively. These results provide preliminary information on the potential use of honey in managing strategies of the disease under greenhouse conditions.
{"title":"Potential use of Apis mellifera L. honey in the management of the cucurbit powdery mildew caused by Podosphaera xanthii (Castagne) under greenhouse conditions","authors":"Juan Antonio Castro-Diego , Carlos Alfonso López-Orona , Verónica Delgado-Pacheco , Miguel Armando López-Beltrán , Nancy Ley-López , Walter Arturo Rubio-Aragón , Jorge Alberto Edeza-Urías","doi":"10.1016/j.ram.2024.10.007","DOIUrl":"10.1016/j.ram.2024.10.007","url":null,"abstract":"<div><div>Powdery mildew by <em>Podosphaera xanthii</em> (Castagne) is a major disease of greenhouse cucurbitaceous crops worldwide. Honey by honeybees has been reported as an antimicrobial for diseases in humans, animals, and plants. The aim of this study was to assess <em>Apis mellifera</em> honey against <em>P. xanthii</em> in cucumber plants. During nine consecutive weeks, four different honey concentrations (2.0%, 2.5%, 3.0% and 3.5%), a chemical control (Azoxystrobin) and an untreated check (water) were evaluated. Except for honey at 2%, every concentration was significantly different from the untreated check. Honey concentrations at 3% and 3.5% were found to be the most effective, and their area under disease progress curve (AUDP) was statistically comparable to that of Azoxystrobin with 1048.3, 642.3 and 575.8 AUDP, representing 72.4%, 83.1% and 84.8% of efficiency compared to the untreated check, respectively. These results provide preliminary information on the potential use of honey in managing strategies of the disease under greenhouse conditions.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 1","pages":"Pages 66-69"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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