Pub Date : 2023-03-23DOI: 10.15446/rcciquifa.v51n3.100424
Bianca Rodrigues Acácio, V. Robles, A. Prada, J. Assis, Tatiane Souza, Hady Keita, S. Neto, Edgar Julián Paredes Gamero, J. R. Rodríguez Amado
Introduction: Until now, few research works have reported the usefulness of Kollicoat MAE® 100P as a film-former polymer for coating nanocapsules and as a matrix former for nanospheres. Aim: To update the current knowledge about the use of Kollicoat MAE® 100P as a film-former polymeric to prepare gastro-resistant nanoparticles. Physicochemical characteristics and functionality of nanoparticles coated with Kollicoat MAE® 100P were reported. Methodology: An exhaustive review was performed (from 1980 to 2021) in various scientific databases like Medline, Scopus, EBSCO and Cambridge. Results: Kollicoat MAE® 100P is a versatile polymer that can be used to prepare gastro-resistant nanoparticles with actives of natural and synthetic origin. This polymer allows producing homogeneous nanoparticles with sizes smaller than 130 nm, and high z-potential, which confers a great stability to nanoparticle systems. On the other side, nanoparticles coated with Kollicoat MAE® 100P combined with plasticizer exhibit a hard and flexible shell, with excellent thermal stability up to 60 °C that dissolve at pH above 5.5. Conclusion: Kollicoat MAE® 100P ris a viable, low-cost, and multifunctional alternative for nanoparticle preparation, however, more studies are needed to develop enhanced nanoparticles with better performances.
{"title":"Kollicoat MAE® 100P as a film former polymer for nanoparticles preparation","authors":"Bianca Rodrigues Acácio, V. Robles, A. Prada, J. Assis, Tatiane Souza, Hady Keita, S. Neto, Edgar Julián Paredes Gamero, J. R. Rodríguez Amado","doi":"10.15446/rcciquifa.v51n3.100424","DOIUrl":"https://doi.org/10.15446/rcciquifa.v51n3.100424","url":null,"abstract":"Introduction: Until now, few research works have reported the usefulness of Kollicoat MAE® 100P as a film-former polymer for coating nanocapsules and as a matrix former for nanospheres. Aim: To update the current knowledge about the use of Kollicoat MAE® 100P as a film-former polymeric to prepare gastro-resistant nanoparticles. Physicochemical characteristics and functionality of nanoparticles coated with Kollicoat MAE® 100P were reported. Methodology: An exhaustive review was performed (from 1980 to 2021) in various scientific databases like Medline, Scopus, EBSCO and Cambridge. Results: Kollicoat MAE® 100P is a versatile polymer that can be used to prepare gastro-resistant nanoparticles with actives of natural and synthetic origin. This polymer allows producing homogeneous nanoparticles with sizes smaller than 130 nm, and high z-potential, which confers a great stability to nanoparticle systems. On the other side, nanoparticles coated with Kollicoat MAE® 100P combined with plasticizer exhibit a hard and flexible shell, with excellent thermal stability up to 60 °C that dissolve at pH above 5.5. Conclusion: Kollicoat MAE® 100P ris a viable, low-cost, and multifunctional alternative for nanoparticle preparation, however, more studies are needed to develop enhanced nanoparticles with better performances.","PeriodicalId":21220,"journal":{"name":"Revista Colombiana de Ciencias Químico-Farmacéuticas","volume":"108 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87629682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-23DOI: 10.15446/rcciquifa.v51n3.107382
Behrouz Seyfinejad, A. Jouyban
Aim: To develop a one-step vortex-assisted liquid-liquid extraction (VALLE) method, without the need for evaporation and reconstitution steps, to establish a rapid and straightforward treatment procedure based on capillary electrophoresis-diode array detection (CE-DAD) for the determination of vanillylmandelic acid (VMA) in human urine. Methodology: Optimization of VALLE and CE-DAD procedures were studied in detail. The effects of various experimental parameters, such as the type of the extraction solvent, sample pH, salt addition, and extraction time were investigated. Also, CE separation conditions including background electrolyte type, concentration, and pH, injection time and separation voltage were optimized as well. Results: A successful separation of VMA was achieved in less than 6 min using a basic background electrolyte composed of 60 mmol·L-1 acetate/ACN (acetonitrile) 50% (v/v) (final apparent pH is 5.73). The linear response was obtained over the concentration range from 1.0 to 14 μg·mL-1. The limit of detection and quantification were 0.30 and 1.0 μg·mL-1, respectively. The intra- and inter-day precisions were found to be less than 4.3%. The extraction recoveries of VMA were between 95%-97%. Conclusion: The developed method is found to be a simple, rapid, and reliable method for quantitative analysis of urinary VMA.
{"title":"A rapid and simple capillary electrophoresis procedure for quantification of vanillylmandelic acid in urine samples","authors":"Behrouz Seyfinejad, A. Jouyban","doi":"10.15446/rcciquifa.v51n3.107382","DOIUrl":"https://doi.org/10.15446/rcciquifa.v51n3.107382","url":null,"abstract":"Aim: To develop a one-step vortex-assisted liquid-liquid extraction (VALLE) method, without the need for evaporation and reconstitution steps, to establish a rapid and straightforward treatment procedure based on capillary electrophoresis-diode array detection (CE-DAD) for the determination of vanillylmandelic acid (VMA) in human urine. Methodology: Optimization of VALLE and CE-DAD procedures were studied in detail. The effects of various experimental parameters, such as the type of the extraction solvent, sample pH, salt addition, and extraction time were investigated. Also, CE separation conditions including background electrolyte type, concentration, and pH, injection time and separation voltage were optimized as well. Results: A successful separation of VMA was achieved in less than 6 min using a basic background electrolyte composed of 60 mmol·L-1 acetate/ACN (acetonitrile) 50% (v/v) (final apparent pH is 5.73). The linear response was obtained over the concentration range from 1.0 to 14 μg·mL-1. The limit of detection and quantification were 0.30 and 1.0 μg·mL-1, respectively. The intra- and inter-day precisions were found to be less than 4.3%. The extraction recoveries of VMA were between 95%-97%. Conclusion: The developed method is found to be a simple, rapid, and reliable method for quantitative analysis of urinary VMA.","PeriodicalId":21220,"journal":{"name":"Revista Colombiana de Ciencias Químico-Farmacéuticas","volume":"16 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90357655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-23DOI: 10.15446/rcciquifa.v51n3.100355
Greyce Hellen Rabelo Cézar, Hemelly Nogueira Guimarães, Joana Stephanie Cidade Santos, Aline Carrilho Menezes, C. Sanches, A. C. Melo, Danilo Donizette Trevisan
Objetivo: construir e validar instrumento para investigar os conhecimentos, atitudes e práticas de estudantes universitários brasileiros sobre a automedicação. Método: estudo metodológico realizado em universidade pública do centro oeste mineiro, Brasil. Um comitê de juízes avaliou clareza, pertinência e abrangência e o pré-teste realizado com público alvo para avaliar compreensão e aceitabilidade. O índice de validade de conteúdo (IVC) foi utilizado para avaliar a proporção de concordância e o coeficiente alfa de Cronbach (α) para mensurar a confiabilidade. Resultados:o instrumento foi composto por 38 itens divididos em três seções: caracterização dos participantes (13 itens); conhecimentos e práticas da automedicação (3 itens); e crenças e atitudes (22 itens).A média do IVC de todas as seções do instrumento foi de 0,97 e o coeficiente alfa de Cronbach da seção crenças e atitudes>0,70. Conclusão: o instrumento foi considerado válido para avaliar conhecimento, prática, crenças e atitudes sobre automedicação em estudantes universitários.
{"title":"Instrumento sobre conhecimento, atitudes e práticas da automedicação em estudantes universitários","authors":"Greyce Hellen Rabelo Cézar, Hemelly Nogueira Guimarães, Joana Stephanie Cidade Santos, Aline Carrilho Menezes, C. Sanches, A. C. Melo, Danilo Donizette Trevisan","doi":"10.15446/rcciquifa.v51n3.100355","DOIUrl":"https://doi.org/10.15446/rcciquifa.v51n3.100355","url":null,"abstract":"Objetivo: construir e validar instrumento para investigar os conhecimentos, atitudes e práticas de estudantes universitários brasileiros sobre a automedicação. Método: estudo metodológico realizado em universidade pública do centro oeste mineiro, Brasil. Um comitê de juízes avaliou clareza, pertinência e abrangência e o pré-teste realizado com público alvo para avaliar compreensão e aceitabilidade. O índice de validade de conteúdo (IVC) foi utilizado para avaliar a proporção de concordância e o coeficiente alfa de Cronbach (α) para mensurar a confiabilidade. Resultados:o instrumento foi composto por 38 itens divididos em três seções: caracterização dos participantes (13 itens); conhecimentos e práticas da automedicação (3 itens); e crenças e atitudes (22 itens).A média do IVC de todas as seções do instrumento foi de 0,97 e o coeficiente alfa de Cronbach da seção crenças e atitudes>0,70. Conclusão: o instrumento foi considerado válido para avaliar conhecimento, prática, crenças e atitudes sobre automedicação em estudantes universitários.","PeriodicalId":21220,"journal":{"name":"Revista Colombiana de Ciencias Químico-Farmacéuticas","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75256361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-23DOI: 10.15446/rcciquifa.v51n3.96862
Sebastián Monge Jiménez, Mariana Piedra Robles, Valeria Portuguez Solano, Daniela Fernández Sequeira, Marianela Chavarría-Rojas, German L Madrigal-Redondo, Eleaneth Baltodano Viales
Objetivo: realizar una revisión bibliográfica sobre la fisiología ano-rectal, el proceso de relajación y contracción del músculo liso del tracto digestivo y posibles materias primas y formulaciones que podrían incorporarse a formulaciones erocosméticas de uso tópico en la región ano-rectal. Métodos: la revisión bibliográfica se realizó utilizando diferentes descriptores y mediante la consulta en las bases de datos ScienceDirect, PubMed, Clinical Key, Google Scholar y Google Patents. Resultados: inicialmente la revisión de la literatura permite comprender la anatomía y fisiología ano-rectal y los mecanismos que modulan la continencia anal, el tono basal del esfínter anal interno y el reflejo inhibitorio rectoanal (RAIR). Posteriormente, se obtuvo información sobre ejemplos de afrodisíacos naturales y se analizaron formulaciones cosméticas utilizadas como lubricantes anales con el fin de estudiar a profundidad los ingredientes de origen natural e identificar su utilidad, mecanismos de acción tópicos y su función dentro de la formulación. Conclusiones: el entendimiento de la fisiología ano-rectal permite el estudio y desarrollo de formulaciones cosméticas con propiedades analgésicas, anestésicas y relajantes, como los lubricantes anales. Productos naturales como la manzanilla, árnica, Aloe vera y mentol han sido estudiados para su uso cosmético y tópico como analgésicos, anestésicos o relajantes, por lo que su utilidad comprobada los hace útiles en el desarrollo de productos erocosméticos destinados para ser utilizados en la región anogenital.
{"title":"Revisión de la fisiología anorrectal y posibles compuestos bioactivos para la erocosmética","authors":"Sebastián Monge Jiménez, Mariana Piedra Robles, Valeria Portuguez Solano, Daniela Fernández Sequeira, Marianela Chavarría-Rojas, German L Madrigal-Redondo, Eleaneth Baltodano Viales","doi":"10.15446/rcciquifa.v51n3.96862","DOIUrl":"https://doi.org/10.15446/rcciquifa.v51n3.96862","url":null,"abstract":"Objetivo: realizar una revisión bibliográfica sobre la fisiología ano-rectal, el proceso de relajación y contracción del músculo liso del tracto digestivo y posibles materias primas y formulaciones que podrían incorporarse a formulaciones erocosméticas de uso tópico en la región ano-rectal. Métodos: la revisión bibliográfica se realizó utilizando diferentes descriptores y mediante la consulta en las bases de datos ScienceDirect, PubMed, Clinical Key, Google Scholar y Google Patents. Resultados: inicialmente la revisión de la literatura permite comprender la anatomía y fisiología ano-rectal y los mecanismos que modulan la continencia anal, el tono basal del esfínter anal interno y el reflejo inhibitorio rectoanal (RAIR). Posteriormente, se obtuvo información sobre ejemplos de afrodisíacos naturales y se analizaron formulaciones cosméticas utilizadas como lubricantes anales con el fin de estudiar a profundidad los ingredientes de origen natural e identificar su utilidad, mecanismos de acción tópicos y su función dentro de la formulación. Conclusiones: el entendimiento de la fisiología ano-rectal permite el estudio y desarrollo de formulaciones cosméticas con propiedades analgésicas, anestésicas y relajantes, como los lubricantes anales. Productos naturales como la manzanilla, árnica, Aloe vera y mentol han sido estudiados para su uso cosmético y tópico como analgésicos, anestésicos o relajantes, por lo que su utilidad comprobada los hace útiles en el desarrollo de productos erocosméticos destinados para ser utilizados en la región anogenital.","PeriodicalId":21220,"journal":{"name":"Revista Colombiana de Ciencias Químico-Farmacéuticas","volume":"120 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90762015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-23DOI: 10.15446/rcciquifa.v51n3.107549
R. Bonilha Dezena, Gustavo Henrique Da Silva, Gisele Mara Silva Gonçalves
Aim: To evaluate the hepatoprotective effects of lycopene pretreatment in paracetamol-induced liver damage (PILD). Methods: Wistar rats were administered oral lycopene (4 mg/kg/day) by gastric lavage for 8 days. Subsequently, 3 g/kg paracetamol was administered on day 8. After 24 and 72 h, animals were euthanized, and intracardiac blood samples were collected to measure levels of aspartate aminotransferase (AST), alanine transaminase (ALT), gamma-glutamyl transferase, and alkaline phosphatase (ALP). In addition, the liver was harvested for histological analyses. Results: Negative and positive control groups (treated with saline or paracetamol on day 8, respectively) were compared with lycopene- and lycopene-paracetamol-treated (lycopene+paracetamol on day 8) groups. Notably, we observed that 24 h after PILD, lycopene treatment significantly reduced serum transaminase (ALT/AST) levels when compared with those in the saline-treated group. Conclusion: Lycopene improved liver recovery following PILD. Although lycopene exhibits antioxidant action and has been indicated for liver diseases, its use must be cautiously undertaken, especially considering the liver patholog y involved, as results may vary for each underlying factor.
{"title":"Hepatoprotective activity of lycopene in experimental paracetamol-induced liver injury in rats","authors":"R. Bonilha Dezena, Gustavo Henrique Da Silva, Gisele Mara Silva Gonçalves","doi":"10.15446/rcciquifa.v51n3.107549","DOIUrl":"https://doi.org/10.15446/rcciquifa.v51n3.107549","url":null,"abstract":"Aim: To evaluate the hepatoprotective effects of lycopene pretreatment in paracetamol-induced liver damage (PILD). Methods: Wistar rats were administered oral lycopene (4 mg/kg/day) by gastric lavage for 8 days. Subsequently, 3 g/kg paracetamol was administered on day 8. After 24 and 72 h, animals were euthanized, and intracardiac blood samples were collected to measure levels of aspartate aminotransferase (AST), alanine transaminase (ALT), gamma-glutamyl transferase, and alkaline phosphatase (ALP). In addition, the liver was harvested for histological analyses. Results: Negative and positive control groups (treated with saline or paracetamol on day 8, respectively) were compared with lycopene- and lycopene-paracetamol-treated (lycopene+paracetamol on day 8) groups. Notably, we observed that 24 h after PILD, lycopene treatment significantly reduced serum transaminase (ALT/AST) levels when compared with those in the saline-treated group. Conclusion: Lycopene improved liver recovery following PILD. Although lycopene exhibits antioxidant action and has been indicated for liver diseases, its use must be cautiously undertaken, especially considering the liver patholog y involved, as results may vary for each underlying factor.","PeriodicalId":21220,"journal":{"name":"Revista Colombiana de Ciencias Químico-Farmacéuticas","volume":"431 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77813823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-23DOI: 10.15446/rcciquifa.v51n3.107383
A. Jouyban
Aim: To report a correlative model to calculate the solubility of ketoprofen in mono-solvents at various temperatures. Methodology: Previously reported solubility data of ketoprofen in a number of mono-solvents at various temperatures are re-analyzed using a recently developed model employing Abraham, Hansen and Catalan parameters as input values. The accuracy of the model is evaluated by computing the standard deviation of residuals (SDRs) and compared with those of previous models. Results: The new model provided very accurate correlation for the solubility of ketoprofen in the investigated mono-solvents at various temperatures. The obtained SDR is 2.20 where the SDRs of previously reported models are 1.54 to 2.56. The new model correlates whole solubility data using a single model, whereas other models correlate the solubility data in each mono-solvent using a separate set of model constants. Conclusion: The trained model provided reasonably accurate results for ketoprofen by using Abraham, Hansen and Catalan parameters of the mono-solvents as input values.
{"title":"Modeling the solubility of ketoprofen in mono-solvents at various temperatures","authors":"A. Jouyban","doi":"10.15446/rcciquifa.v51n3.107383","DOIUrl":"https://doi.org/10.15446/rcciquifa.v51n3.107383","url":null,"abstract":"Aim: To report a correlative model to calculate the solubility of ketoprofen in mono-solvents at various temperatures. Methodology: Previously reported solubility data of ketoprofen in a number of mono-solvents at various temperatures are re-analyzed using a recently developed model employing Abraham, Hansen and Catalan parameters as input values. The accuracy of the model is evaluated by computing the standard deviation of residuals (SDRs) and compared with those of previous models. Results: The new model provided very accurate correlation for the solubility of ketoprofen in the investigated mono-solvents at various temperatures. The obtained SDR is 2.20 where the SDRs of previously reported models are 1.54 to 2.56. The new model correlates whole solubility data using a single model, whereas other models correlate the solubility data in each mono-solvent using a separate set of model constants. Conclusion: The trained model provided reasonably accurate results for ketoprofen by using Abraham, Hansen and Catalan parameters of the mono-solvents as input values.","PeriodicalId":21220,"journal":{"name":"Revista Colombiana de Ciencias Químico-Farmacéuticas","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90557401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-23DOI: 10.15446/rcciquifa.v51n3.100452
Camilla De Menezes, A. Pérez, J. Sousa, K. Ramos, H. Diniz-Neto, A. Oliveira-Filho, E. Lima
Introduction: Cladophialophora carrionii is one of the most frequent etiologic agents of human chromoblastomycosis, a chronic cutaneous disease. Such fungal infections are difficult to treat and underlines the need for new antifungal agents. Citral is a monoterpene with known pharmacological properties, including antimi-crobial activity. Aims: To investigate the antifungal activity of citral against strains of C. carrionii. Methodology: The minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) were determined by broth microdilution techniques. Citral was tested to evaluate its effects on C. carrionii mycelia growth and germination of fungal conidia. Next, it was investigated the possible citral action on cell walls (sorbitol assay) and cell membranes (ergosterol binding assay). Results: The MIC50 and MFC50 of citral were respectively 128 μg/mL and 512 μg/mL. The study shows that citral displayed in vitro antifungal potential against strains of C. carrionii, where it was capable of inhibiting both its mycelial growth and germination of conidia for this fungus, whilst affecting the structure of fungal cell membranes. Citral’s mechanism of action involves binding to ergosterol. Further study is needed to completely describe its effects before clinical use as a therapeutic antifungal agent.
{"title":"Evaluation of the antifungal activity and mode of action of citral against Cladophialophora carrionii.","authors":"Camilla De Menezes, A. Pérez, J. Sousa, K. Ramos, H. Diniz-Neto, A. Oliveira-Filho, E. Lima","doi":"10.15446/rcciquifa.v51n3.100452","DOIUrl":"https://doi.org/10.15446/rcciquifa.v51n3.100452","url":null,"abstract":"Introduction: Cladophialophora carrionii is one of the most frequent etiologic agents of human chromoblastomycosis, a chronic cutaneous disease. Such fungal infections are difficult to treat and underlines the need for new antifungal agents. Citral is a monoterpene with known pharmacological properties, including antimi-crobial activity. Aims: To investigate the antifungal activity of citral against strains of C. carrionii. Methodology: The minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) were determined by broth microdilution techniques. Citral was tested to evaluate its effects on C. carrionii mycelia growth and germination of fungal conidia. Next, it was investigated the possible citral action on cell walls (sorbitol assay) and cell membranes (ergosterol binding assay). Results: The MIC50 and MFC50 of citral were respectively 128 μg/mL and 512 μg/mL. The study shows that citral displayed in vitro antifungal potential against strains of C. carrionii, where it was capable of inhibiting both its mycelial growth and germination of conidia for this fungus, whilst affecting the structure of fungal cell membranes. Citral’s mechanism of action involves binding to ergosterol. Further study is needed to completely describe its effects before clinical use as a therapeutic antifungal agent.","PeriodicalId":21220,"journal":{"name":"Revista Colombiana de Ciencias Químico-Farmacéuticas","volume":"89 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83868579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-23DOI: 10.15446/rcciquifa.v51n3.107328
W. Lima, Patrícia Alcântara Cândido, Adriano de Paula Sabino, V. Nascimento Cardoso, Simone Odília Antunes Fernandes
Introduction: Inflammatory bowel diseases (IBDs) are idiopathic inflammations of the colon, which can often undergo remission with the use of specific anti-TNF antibodies such as the infliximab. Although that the therapeutic monitoring can provide valuable insight into the possible etiolog y of unfavorable outcomes and allow for an appropriate management strateg y for these patients, currently none technique or biomarker have proved ideal for the evaluation of therapeutic benefices of anti-TNF antibodies in IBDs. Aim: To summarise current knowledge on the role of serum adipokines as potential biomarkers for the therapeutic monitoring of patients with IBDs under infliximab. Methods: A systematic review was carried out in the PubMed/MEDLINE, Cochrane Library, Scopus and Biblioteca Virtual em Saúde databases. Next, the meta-analysis was performed with the mean values of serum adipokine levels in patients with IBDs before and after the use of infliximab using the Review Manager (RevMan) ® 5.3 software. Results: Three studies that together included 58 patients diagnosed with IBDs and treated with infliximab at 5 mg/kg were selected. According to the quantitative analysis, serum leptin levels were significantly increased after the use of infliximab (p-value=0.01; Heterogeneity: I2=61%), which is correlated with the clinical remission of the disease. In addition, the circulating adiponectin (p-value=0.006; Heterogeneity: I2=0%) and resistin (p-value=0.009; Heterogeneity: I2=93%) concentrations were both reduced after the clinical remission of IBD with the use of infliximab. Conclusion: Serum leptin levels are significantly increased, while circulating adiponectin and resistin is reduced among IBD patients after the use of infliximab.
简介:炎症性肠病(IBDs)是结肠的特发性炎症，通常可以通过使用特异性抗tnf抗体如英夫利昔单抗来缓解。尽管治疗性监测可以为不良结果的可能病因提供有价值的见解，并为这些患者提供适当的管理策略，但目前还没有一种技术或生物标志物被证明是评估ibd中抗tnf抗体治疗效果的理想方法。目的:总结目前关于血清脂肪因子作为ibd患者英夫利昔单抗治疗监测的潜在生物标志物的作用的知识。方法:对PubMed/MEDLINE、Cochrane Library、Scopus和Biblioteca Virtual em Saúde数据库进行系统综述。接下来，使用Review Manager (RevMan)®5.3软件对ibd患者使用英夫利昔单抗前后的血清脂肪因子水平平均值进行meta分析。结果:3项研究共纳入58例诊断为ibd并以5 mg/kg剂量英夫利昔单抗治疗的患者。定量分析显示，使用英夫利昔单抗后血清瘦素水平显著升高(p值=0.01;异质性:I2=61%)，这与疾病的临床缓解相关。此外，循环脂联素(p值=0.006;异质性:I2=0%)和抵抗素(p值=0.009;异质性:I2=93%)使用英夫利昔单抗后IBD临床缓解后浓度均降低。结论:IBD患者使用英夫利昔单抗后血清瘦素水平明显升高，而循环脂联素和抵抗素水平明显降低。
{"title":"Serum adipokines as biomarkers for the therapeutic monitoring of patients with inflammatory bowel diseases (IBDs) treated with infliximab: a systematic review and meta-analysis","authors":"W. Lima, Patrícia Alcântara Cândido, Adriano de Paula Sabino, V. Nascimento Cardoso, Simone Odília Antunes Fernandes","doi":"10.15446/rcciquifa.v51n3.107328","DOIUrl":"https://doi.org/10.15446/rcciquifa.v51n3.107328","url":null,"abstract":"Introduction: Inflammatory bowel diseases (IBDs) are idiopathic inflammations of the colon, which can often undergo remission with the use of specific anti-TNF antibodies such as the infliximab. Although that the therapeutic monitoring can provide valuable insight into the possible etiolog y of unfavorable outcomes and allow for an appropriate management strateg y for these patients, currently none technique or biomarker have proved ideal for the evaluation of therapeutic benefices of anti-TNF antibodies in IBDs. Aim: To summarise current knowledge on the role of serum adipokines as potential biomarkers for the therapeutic monitoring of patients with IBDs under infliximab. Methods: A systematic review was carried out in the PubMed/MEDLINE, Cochrane Library, Scopus and Biblioteca Virtual em Saúde databases. Next, the meta-analysis was performed with the mean values of serum adipokine levels in patients with IBDs before and after the use of infliximab using the Review Manager (RevMan) ® 5.3 software. Results: Three studies that together included 58 patients diagnosed with IBDs and treated with infliximab at 5 mg/kg were selected. According to the quantitative analysis, serum leptin levels were significantly increased after the use of infliximab (p-value=0.01; Heterogeneity: I2=61%), which is correlated with the clinical remission of the disease. In addition, the circulating adiponectin (p-value=0.006; Heterogeneity: I2=0%) and resistin (p-value=0.009; Heterogeneity: I2=93%) concentrations were both reduced after the clinical remission of IBD with the use of infliximab. Conclusion: Serum leptin levels are significantly increased, while circulating adiponectin and resistin is reduced among IBD patients after the use of infliximab.","PeriodicalId":21220,"journal":{"name":"Revista Colombiana de Ciencias Químico-Farmacéuticas","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90780025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-24DOI: 10.15446/rcciquifa.v51n1.94145
Filipe De Castro Faria, Carina Alves Ferreira, Vinícius Lopes Cantuária, K. C. Kato, Fernando Armini Ruela, Fernando Costa Archanjo, Helen Rodrigues Martins, C. Grael
Os extratos etanólico e hidroalcoólico de Ageratum fastigiatum são usados na medicina popular como agentes antiinflamatório e analgésico. Neste estudo, o extrato etanólico da parte aérea da planta e suas frações foram avaliados quanto à sua toxicidade em Artemia salina e nas células do tecido conjuntivo de camundongos (L929). O extrato foi particionado e suas fases hexânica, diclorometânica e hidroalcoólica foram submetidas ao mesmo teste. A triagem fitoquímica das fases do extrato foi realizada por meio de testes visando a detecção de classes de metabólitos secundários e GC-MS. Em relação a A. salina, a fase hidroalcoólica apresentou a maior toxicidade (CL50, 1,33 mg/mL) e o extrato etanólico bruto foi o menos tóxico (CL50, 4,81 mg / mL). No ensaio de células L929, a fase de diclorometânica foi a mais tóxica (95,48% de redução na viabilidade celular; LC50, 11,39 μg / mL), enquanto a fase hidroalcoólica foi a menos tóxica (porcentagem de morte celular, 55,67% –19,38%; LC50 , 174,20 μg / mL). O estudo fitoquímico indicou a provável presença de alcalóides, cumarinas, saponinas, triterpenos / esteróides e taninos. A análise por GC-MS identificou a presença de terpenóides e um derivado de licopsamina (alcalóide pirrolizidínico). Esses resultados sugerem que o extrato etanólico de A. fastigiatum possui constituintes (ou seja, compostos fenólicos) que corroboram seu uso na medicina popular como agente antiinflamatório. No entanto, a toxicidade detectada e a presença da 3¢-acetil licopsamina (um marcador quimiotaxonômico do gênero, que é hepatotóxico) indicam que essa planta medicinal deve ser usada com cautela.
{"title":"Phytochemical screening and evaluation of the toxic potential of ethanolic extract and fractions of Ageratum fastigiatum","authors":"Filipe De Castro Faria, Carina Alves Ferreira, Vinícius Lopes Cantuária, K. C. Kato, Fernando Armini Ruela, Fernando Costa Archanjo, Helen Rodrigues Martins, C. Grael","doi":"10.15446/rcciquifa.v51n1.94145","DOIUrl":"https://doi.org/10.15446/rcciquifa.v51n1.94145","url":null,"abstract":"Os extratos etanólico e hidroalcoólico de Ageratum fastigiatum são usados na medicina popular como agentes antiinflamatório e analgésico. Neste estudo, o extrato etanólico da parte aérea da planta e suas frações foram avaliados quanto à sua toxicidade em Artemia salina e nas células do tecido conjuntivo de camundongos (L929). O extrato foi particionado e suas fases hexânica, diclorometânica e hidroalcoólica foram submetidas ao mesmo teste. A triagem fitoquímica das fases do extrato foi realizada por meio de testes visando a detecção de classes de metabólitos secundários e GC-MS. Em relação a A. salina, a fase hidroalcoólica apresentou a maior toxicidade (CL50, 1,33 mg/mL) e o extrato etanólico bruto foi o menos tóxico (CL50, 4,81 mg / mL). No ensaio de células L929, a fase de diclorometânica foi a mais tóxica (95,48% de redução na viabilidade celular; LC50, 11,39 μg / mL), enquanto a fase hidroalcoólica foi a menos tóxica (porcentagem de morte celular, 55,67% –19,38%; LC50 , 174,20 μg / mL). O estudo fitoquímico indicou a provável presença de alcalóides, cumarinas, saponinas, triterpenos / esteróides e taninos. A análise por GC-MS identificou a presença de terpenóides e um derivado de licopsamina (alcalóide pirrolizidínico). Esses resultados sugerem que o extrato etanólico de A. fastigiatum possui constituintes (ou seja, compostos fenólicos) que corroboram seu uso na medicina popular como agente antiinflamatório. No entanto, a toxicidade detectada e a presença da 3¢-acetil licopsamina (um marcador quimiotaxonômico do gênero, que é hepatotóxico) indicam que essa planta medicinal deve ser usada com cautela.","PeriodicalId":21220,"journal":{"name":"Revista Colombiana de Ciencias Químico-Farmacéuticas","volume":"118 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77989213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-09DOI: 10.15446/rcciquifa.v51n1.93437
Gisele Mara Silva Gonçalve, P. Barros, Gustavo Henrique Da Silva, Júlia Ferreira Watanabe, A. Eisinger
Introduction: Carthamus oil is a compound that has the potential to be used in numerous applications due to its anti-inflammatory, antioxidant, immunomodulatory and neuroprotective effects. Chromium picolinate has been indicated for the control of insulin resistance. Aim: To evaluate the effect of Carthamus oil (30 mg/kg) and chromium picolinate (5 µg/kg) interaction with oral glyburide in chemically diabetes-induced Wistar rats and its influence on drug therapy. Method: Diabetes mellitus was induced with streptozotocin, and the animals were randomized into experimental groups (n= 6/group), who received gastric gavage treatments for ten days, G1: control, G2: diabetic and received glyburide, G3: diabetic and received the interaction of Carthamus oil and chromium picolinate, G4: diabetic and received the interaction of glyburide, Carthamus oil and chromium picolinate. After the treatment period, fasting blood glucose, post-sucrose blood glucose, total cholesterol and triglyceride levels, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in blood serum were compared, in addition to urine analysis. Results: In this study, the only altered parameters were the post-sucrose blood glucose measurement with the lowest result for G4 (P <0.05), and the ALT measurement, with lower values for G4 (P <0.05) compared to G1. Conclusion: It can be concluded that the unprecedented interaction of Carthamus oil, chromium picolinate and glyburide contributed to the reduction of blood glucose and serum levels of ALT in diabetic rats and is promising for future studies in humans.
{"title":"Interaction of Carthamus oil, chromium picolinate and glyburide in blood glucose and its impact on liver enzymes and lipid profile in diabetes-induced rats","authors":"Gisele Mara Silva Gonçalve, P. Barros, Gustavo Henrique Da Silva, Júlia Ferreira Watanabe, A. Eisinger","doi":"10.15446/rcciquifa.v51n1.93437","DOIUrl":"https://doi.org/10.15446/rcciquifa.v51n1.93437","url":null,"abstract":"Introduction: Carthamus oil is a compound that has the potential to be used in numerous applications due to its anti-inflammatory, antioxidant, immunomodulatory and neuroprotective effects. Chromium picolinate has been indicated for the control of insulin resistance. Aim: To evaluate the effect of Carthamus oil (30 mg/kg) and chromium picolinate (5 µg/kg) interaction with oral glyburide in chemically diabetes-induced Wistar rats and its influence on drug therapy. Method: Diabetes mellitus was induced with streptozotocin, and the animals were randomized into experimental groups (n= 6/group), who received gastric gavage treatments for ten days, G1: control, G2: diabetic and received glyburide, G3: diabetic and received the interaction of Carthamus oil and chromium picolinate, G4: diabetic and received the interaction of glyburide, Carthamus oil and chromium picolinate. After the treatment period, fasting blood glucose, post-sucrose blood glucose, total cholesterol and triglyceride levels, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in blood serum were compared, in addition to urine analysis. Results: In this study, the only altered parameters were the post-sucrose blood glucose measurement with the lowest result for G4 (P <0.05), and the ALT measurement, with lower values for G4 (P <0.05) compared to G1. Conclusion: It can be concluded that the unprecedented interaction of Carthamus oil, chromium picolinate and glyburide contributed to the reduction of blood glucose and serum levels of ALT in diabetic rats and is promising for future studies in humans.","PeriodicalId":21220,"journal":{"name":"Revista Colombiana de Ciencias Químico-Farmacéuticas","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87789109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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