Molecular Nutrition & Food Research最新文献_第9页

Puerarin Attenuates Insulin Resistance by Inhibiting Endoplasmic Reticulum Stress and Suppresses Inflammation by Modulating the JNK and IKKβ/NF-κB Pathways in Epididymal White Adipose Tissue of Mice on a High-Fat Diet 葛根素通过抑制内质网应激减轻胰岛素抵抗，并通过调节高脂饮食小鼠表皮白色脂肪组织中的JNK和IKKβ/NF-κB通路抑制炎症。

IF 4.5 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research

Pub Date : 2024-07-28 DOI: 10.1002/mnfr.202400003

Jie Sun, Yan Liu, Jinjin Zhang, Huilin Shi, Rujiao Jiang, Meihua Guo, Yilin Liu, Bo Liu, Ning Wang, Rui Ma, Danna Zhang, Fang Zhang, Shujing Wang, Yingjie Wu

Scope

Obesity is associated with insulin resistance (IR), which is characterized by endoplasmic reticulum (ER) stress in multiple organs. ER stress in adipose tissue causes metabolic disturbances and activates inflammatory signaling pathways. Puerarin, an isoflavone extracted from Pueraria lobata, exhibits antioxidant, anti-inflammatory, and antidiabetic effects. This study explores the potential mechanisms underlying puerarin's role in mitigating insulin resistance in high-fat diet (HFD)-induced obese mice.

Methods and results

In this study, insulin resistant in mice is induced by a high-fat diet, followed by treatment with puerarin. The results demonstrate that puerarin effectively attenuates insulin resistance, including weight loss, improvement of glucose tolerance and insulin sensitivity, and activation of insulin signaling pathway. Additionally, puerarin administration suppresses ER stress by down-regulation of ATF6, ATF4, CHOP, GRP78 expressions in epididymal white adipose tissue (eWAT), along with decreased phosphorylation IRE1α, PERK, and eIF2α. Furthermore, puerarin exerts anti-inflammatory effects by inhibiting JNK and IKKβ/NF-κB pathways, leading to reduction of TNF-α and IL-6.

Conclusion

These findings suggest that puerarin mitigates insulin resistance by inhibiting ER stress and suppressing inflammation through the JNK and IKKβ/NF-κB pathways. This highlights the promising clinical application of puerarin in the treatment of insulin resistance.

范围：肥胖与胰岛素抵抗（IR）有关，而胰岛素抵抗的特征是多个器官的内质网（ER）应激。脂肪组织中的内质网应激会导致新陈代谢紊乱，并激活炎症信号通路。葛根素是从葛根中提取的一种异黄酮，具有抗氧化、抗炎和抗糖尿病作用。本研究探讨了葛根素缓解高脂饮食（HFD）诱导的肥胖小鼠胰岛素抵抗的潜在机制：本研究通过高脂饮食诱导小鼠胰岛素抵抗，然后用葛根素治疗。结果表明，葛根素能有效减轻胰岛素抵抗，包括减轻体重、改善糖耐量和胰岛素敏感性以及激活胰岛素信号通路。此外，葛根素通过下调附睾白色脂肪组织（eWAT）中ATF6、ATF4、CHOP和GRP78的表达，以及减少IRE1α、PERK和eIF2α的磷酸化，抑制ER应激。此外，葛根素通过抑制JNK和IKKβ/NF-κB途径发挥抗炎作用，导致TNF-α和IL-6的减少：这些研究结果表明，葛根素可通过JNK和IKKβ/NF-κB途径抑制ER应激和炎症，从而缓解胰岛素抵抗。这凸显了葛根素在治疗胰岛素抵抗方面的临床应用前景。

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引用次数: 0

Issue Information: Mol. Nutr. Food Res. 14'24 发行信息：Mol.Nutr.Food Res.

IF 4.5 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research

Pub Date : 2024-07-26 DOI: 10.1002/mnfr.202470025

引用次数: 0

Global Bibliometric and Visualized Analysis of Research on Lactoferrin from 1978 to 2024 1978 至 2024 年全球乳铁蛋白研究文献计量和可视化分析。

IF 4.5 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research

Pub Date : 2024-07-23 DOI: 10.1002/mnfr.202400379

Hong Gu, Yiming Wang, Yating Wang, Liyi Ding, Wenru Huan, Yuting Yang, Fang Fang, Weiwei Cui

Scope

Lactoferrin (LF) is an iron-bound protein with a molecular weight of about 80 kDa. LF has many biological functions such as antibacterial, antiviral, immunomodulatory, and anticancer. The purpose of this study is to explore the research trend of LF through bibliometric analysis.

Methods and results

The search is conducted in the Web of Science Core Collection database, and then the publications information of LF related literature is exported. Based on CiteSpace and VOSviewer software, countries, institutions, authors, journals, keywords, and so on are analyzed. Since 1987, a total of 9382 literature have been included, and the number of papers related to LF has increased year by year. These publications come mainly from 124 countries and 725 institutions. Of the 1256 authors analyzed, Valenti Piera is the one with the most publications. The burst strength of gut microbiota, antioxidant, nanoparticles, and in vitro digestion are 21.3, 15.63, 23.03, and 13.51, respectively. They represent the frontier of research in this field and are developing rapidly.

Conclusion

This study shows that LF has important research value. The study of LF nanoparticles and the effects of LF on the gut microbiota are an emerging field that helps to explore new research directions.

范围：乳铁蛋白（LF）是一种分子量约为 80 kDa 的铁结合蛋白。乳铁蛋白具有抗菌、抗病毒、免疫调节和抗癌等多种生物学功能。本研究的目的是通过文献计量学分析探讨 LF 的研究趋势：在 Web of Science Core Collection 数据库中进行检索，然后导出 LF 相关文献的发表信息。基于 CiteSpace 和 VOSviewer 软件，对国家、机构、作者、期刊、关键词等进行分析。自 1987 年以来，共收录文献 9382 篇，与 LF 相关的论文数量逐年增加。这些文献主要来自 124 个国家和 725 个机构。在分析的 1256 位作者中，Valenti Piera 是发表论文最多的一位。肠道微生物群、抗氧化剂、纳米颗粒和体外消化的突发强度分别为 21.3、15.63、23.03 和 13.51。它们代表了这一领域的研究前沿，发展迅速：本研究表明，LF 具有重要的研究价值。LF 纳米颗粒的研究以及 LF 对肠道微生物群的影响是一个新兴领域，有助于探索新的研究方向。

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引用次数: 0

Lactobacillus rhamnosus GG and Tannic Acid Synergistically Promote the Gut Barrier Integrity in a Rat Model of Experimental Diarrhea via Selective Immunomodulatory Cytokine Targeting 鼠李糖乳杆菌 GG 和单宁酸通过选择性靶向免疫调节细胞因子协同促进实验性腹泻大鼠模型的肠道屏障完整性

IF 4.5 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research

Pub Date : 2024-07-21 DOI: 10.1002/mnfr.202400295

Lamiaa M. Shawky, Sahar M. Abo El Wafa, Maged Behery, Mohamed H. Bahr, Mahmoud Tawfiq Abu Alnasr, Ahmed A. Morsi

Scope

Diarrhea is a common health issue that contributes to a significant annual death rate among children and the elderly worldwide. The anti-diarrheal activity of Lactobacillus rhamnosus GG (LGG) and tannic acid (TA), alone or combined, is examined, in addition to their effect on intestinal barrier integrity.

Methods and results

Fifty-six adult male Wistar rats are randomly assigned into seven groups: control, LGG alone, TA alone, diarrhea model, diarrhea+LGG, diarrhea+TA, and diarrhea+LGG+TA-treated groups. Diarrhea is induced by high-lactose diet (HLD) consumption. LGG (1x10⁹ CFU/rat) and TA (100 mg Kg⁻¹ d⁻¹) were given orally 4 days after HLD feeding and continued for 10 days. Ileum specimens are processed for biochemical analysis of the local intestinal cytokines, polymerase chain reaction (PCR), and histological study. Also, immunohistochemistry-based identification of Proliferating Cell Nuclear Antigen (PCNA) and zonula occludens 1 (ZO-1) is performed. Compared to the diarrhea model group, both treatments maintain the intestinal mucosal structure and proliferative activity and preserve ZO-1 expression, with the combination group showing the maximal effect. However, LGG-treated diarrheic rats show a remarkable decrease in the intestinal tissue concentrations of tumor necrosis factor-alpha (TNF-α) and nuclear factor Kappa beta (NF-κB); meanwhile, TA treatment leads to a selective decrease of interferon-gamma (INF-γ) and transforming growth factor-beta (TGF-β1).

Conclusion

Individual LGG and TA treatments significantly alleviate diarrhea, probably through a selective immunomodulatory cytokine-dependent mechanism, while the combination of both synergistically maintains the intestinal mucosa by keeping the intestinal epithelial barrier function and regenerative capability.

范围：腹泻是一个常见的健康问题，每年造成全球儿童和老年人的大量死亡。本文研究了鼠李糖乳杆菌 GG（LGG）和单宁酸（TA）单独或联合使用的止泻活性，以及它们对肠道屏障完整性的影响：将 56 只成年雄性 Wistar 大鼠随机分为 7 组：对照组、单独 LGG 组、单独 TA 组、腹泻模型组、腹泻+LGG 组、腹泻+TA 组和腹泻+LGG+TA 处理组。腹泻由食用高乳糖饮食（HLD）诱发。在喂食 HLD 4 天后口服 LGG（1x109 CFU/只大鼠）和 TA（100 mg Kg-1 d-1），并持续 10 天。对回肠标本进行局部肠道细胞因子生化分析、聚合酶链反应（PCR）和组织学研究。此外，还对增殖细胞核抗原（PCNA）和闭锁带1（ZO-1）进行免疫组化鉴定。与腹泻模型组相比，两种治疗方法都能维持肠粘膜结构和增殖活性，并保留 ZO-1 的表达，其中联合治疗组的效果最佳。然而，LGG治疗的腹泻大鼠肠道组织中肿瘤坏死因子-α（TNF-α）和核因子卡巴β（NF-κB）的浓度显著下降；同时，TA治疗导致γ干扰素（INF-γ）和转化生长因子-β（TGF-β1）选择性下降：结论：单独使用 LGG 和 TA 治疗可明显缓解腹泻，这可能是通过选择性免疫调节细胞因子依赖机制实现的，而两者联合使用可协同保持肠上皮屏障功能和再生能力，从而维护肠粘膜。

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引用次数: 0

Unlocking Dietary Strategies to Combat Neurological Disorders 揭秘防治神经系统疾病的饮食策略

IF 4.5 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research

Pub Date : 2024-07-19 DOI: 10.1002/mnfr.202400381

Ariana Soares Dias Portela, Ruth Iban-Arias, Vrinda Saxena, Sibilla Masieri, Giulio Maria Pasinetti

The acquisition of novel insights derived from the biological and genetic profiles of patients will pave the way for tailored interventions and guidance, facilitated by pioneering methodologies and investigations in research. Such advancements will lead to shifts in dietary patterns and proactively mitigate the onset of neurological disorders.

从患者的生物和遗传特征中获得新的见解，将为量身定制的干预和指导铺平道路。这些进步将导致饮食模式的转变，并积极缓解神经系统疾病的发病。

引用次数: 0

Issue Information: Mol. Nutr. Food Res. 13'24 发行信息：Mol.Nutr.13'24

IF 4.5 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research

Pub Date : 2024-07-19 DOI: 10.1002/mnfr.202470024

引用次数: 0

The Allergen Profile of Two Edible Insect Species—Acheta domesticus and Hermetia illucens 两种可食用昆虫--家蝇和白头翁的过敏原概况

IF 4.5 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research

Pub Date : 2024-07-18 DOI: 10.1002/mnfr.202300811

Shaymaviswanathan Karnaneedi, Elecia B. Johnston, Utpal Bose, Angéla Juhász, James A. Broadbent, Thimo Ruethers, Emily M. Jerry, Sandip D. Kamath, Vachiranee Limviphuvadh, Sally Stockwell, Keren Byrne, Dean Clarke, Michelle L. Colgrave, Sebastian Maurer-Stroh, Andreas L. Lopata

Scope

Edible insect proteins are increasingly introduced as an alternative sustainable food source to address the world's need to feed the growing population. Tropomyosin is the main insect allergen; however, additional potential allergens are not well characterized and the impact of extraction procedures on immunological reactivity is unknown.

Methods and results

Proteins from different commercial food products derived from cricket (Acheta domesticus) and black soldier fly (BSF) (Hermetia illucens) are extracted using five different extraction buffers. The proteins are analyzed by SDS-PAGE and immunoblotting using allergen-specific antibodies and crustacean allergic patient sera. IgE binding bands are analyzed by mass spectrometry as well as the complete allergen profile of all 30 extracts. Urea-based buffers are most efficient in extracting insect allergens. Shrimp-specific antibody cross-reactivity to tropomyosin from cricket and BSF indicates high sequence and structural similarity between shrimp and insects. Additional unique allergens are identified in both species, including hemocyanin, vitellogenin, HSP20, apolipophorin-III, and chitin-binding protein.

Conclusions

Identifying potential allergenic proteins and their isoforms in cricket and BSF requires specific extraction approaches using urea-based methods. While tropomyosin is the most abundant and immunoreactive allergen, seven unique allergens are identified, highlighting the need for insect species-specific allergen detection in food products.

范围：可食用昆虫蛋白作为一种可替代的可持续食物来源，越来越多地被采用，以满足世界上不断增长的人口对食物的需求。托布霉素是主要的昆虫过敏原；然而，其他潜在过敏原的特征还不明确，提取程序对免疫反应性的影响也不清楚：使用五种不同的提取缓冲液提取了来自蟋蟀（Acheta domesticus）和黑兵蝇（BSF）（Hermetia illucens）的不同商业食品中的蛋白质。使用过敏原特异性抗体和甲壳类过敏患者血清对蛋白质进行 SDS-PAGE 和免疫印迹分析。通过质谱分析 IgE 结合带以及所有 30 种提取物的完整过敏原图谱。基于尿素的缓冲液提取昆虫过敏原的效率最高。虾特异性抗体与蟋蟀和 BSF 的肌球蛋白的交叉反应表明，虾与昆虫的序列和结构高度相似。在这两个物种中还发现了其他独特的过敏原，包括血蓝蛋白、卵黄素、HSP20、apolipophorin-III和几丁质结合蛋白：鉴定蟋蟀和BSF中潜在的致敏蛋白质及其同工形式需要使用基于尿素的特定提取方法。虽然肌球蛋白是含量最高、免疫反应最强的过敏原，但也发现了七种独特的过敏原，这凸显了在食品中检测昆虫物种特异性过敏原的必要性。

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引用次数: 0

Fructooligosaccharides Ameliorate Renal Injury and Dysfunction Through the Modulation of Gut Dysbiosis, Inhibition of Renal Inflammation, Oxidative Stress, Fibrosis, and Improve Organic Anion Transporter 3 Function in an Obese Rat Model 果寡糖通过调节肠道菌群失调、抑制肾脏炎症、氧化应激和纤维化以及改善肥胖大鼠模型中有机阴离子转运体 3 的功能，改善肾脏损伤和功能障碍。

IF 4.5 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research

Pub Date : 2024-07-17 DOI: 10.1002/mnfr.202400191

Nattavadee Pengrattanachot, Laongdao Thongnak, Sasivimon Promsan, Nichakorn Phengpol, Prempree Sutthasupha, Jiraporn Tocharus, Anusorn Lungkaphin

Scope

High-fat diet (HFD) consumption causes obesity and gut dysbiosis which induces kidney injury. It has been reported that prebiotics improve gut dysbiosis and insulin sensitivity and decelerate the progression of kidney disease. This study investigates the impact of fructooligosaccharides (FOS) on renoprotection and the prevention of gut dysbiosis and intestinal barrier injury in obese rats.

Methods and results

Wistar rats are treated with HFD for 16 weeks. Then, the HFD fed rats (HF) are given FOS 1 g day⁻¹ (HFFOS1), 2 g day⁻¹ (HFFOS2), or metformin 30 mg kg⁻¹ day⁻¹ (HFMET), by intragastric feeding for 8 weeks. Blood, urine, feces, kidney, and intestine are collected to determine the metabolic changes, gut dysbiosis, and the expression of proteins involved in kidney and intestinal injury. FOS can attenuate insulin resistance and hypercholesterolemia concomitant with the inhibition of renal inflammation, oxidative stress, fibrosis, and apoptosis, which are related to the deceleration of the overexpression of renal Toll-like receptor 4 (TLR4) and NADPH oxidase (NOX4). Moreover, FOS shows a greater efficacy than metformin in the reduction of the intestinal injury and loss of tight junction proteins induced by HFD.

Conclusion

FOS may be used as a supplement for therapeutic purposes in an obese condition to improve intestinal integrity and prevent renal complications.

范围高脂饮食（HFD）会导致肥胖和肠道菌群失调，从而诱发肾损伤。据报道，益生元可改善肠道菌群失调和胰岛素敏感性，并减缓肾脏疾病的进展。本研究探讨了果寡糖（FOS）对肥胖大鼠肾脏保护和预防肠道菌群失调及肠道屏障损伤的影响：对 Wistar 大鼠进行为期 16 周的 HFD 治疗。然后，通过胃内喂食给予 FOS 1 克/天-1（HFFOS1）、2 克/天-1（HFFOS2）或二甲双胍 30 毫克/公斤-1 天-1（HFMET），连续喂食 8 周。收集血液、尿液、粪便、肾脏和肠道，以确定代谢变化、肠道菌群失调以及参与肾脏和肠道损伤的蛋白质的表达。FOS 可减轻胰岛素抵抗和高胆固醇血症，同时抑制肾脏炎症、氧化应激、纤维化和细胞凋亡，这与减缓肾脏 Toll 样受体 4（TLR4）和 NADPH 氧化酶（NOX4）的过度表达有关。此外，与二甲双胍相比，FOS 在减少高氟酸膳食引起的肠道损伤和紧密连接蛋白损失方面表现出更大的功效：结论：FOS 可用作肥胖症患者的治疗补充剂，以改善肠道完整性并预防肾脏并发症。

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引用次数: 0

A Food Odorant, α-Ionone, Inhibits Skin Cancer Tumorigenesis by Activation of OR10A6 食物气味剂α-壬酮通过激活 OR10A6 抑制皮肤癌肿瘤发生

IF 4.5 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research

Pub Date : 2024-07-17 DOI: 10.1002/mnfr.202400085

Ye Eun Yoon, Young Jae Jung, Sung-Joon Lee

Scope

This study aims to investigate the anticancer properties of α-ionone in squamous cell carcinoma (SCC).

Methods and results

The expression of OR10A6 together with olfactory receptor signaling components is demonstrated in A431 human SCC cells via RT-PCR and qRT-PCR analysis. OR10A6 activation in A431 cells using the ligand α-ionone inhibits proliferation and migration but induces apoptosis which is confirmed by proliferation assay, colony formation, and western blotting. The mechanism involves the core proteins of the Hippo pathway, where the phosphorylation of large tumor suppressor kinase (LATS), yes-associated protein (YAP), and transcriptional coactivator with PDZ-binding motif (TAZ) is confirmed by western blotting. However, the anticancer effects of α-ionone are abrogated in A431 cells with OR10A6 gene knockdown. In A431 xenograft mouse model, the injection of α-ionone suppresses tumor growth, induces apoptosis, and increases phosphorylation of the LATS-YAP-TAZ signaling axis in the Hippo pathway. None of these effects are observed in xenografted tumors with OR10A6 gene knockdown.

Conclusion

These findings collectively demonstrate that activation of ectopic OR OR10A6 by α-ionone in SCC cells stimulates the Hippo pathway and suppresses tumorigenesis both in vitro and in vivo, suggesting a novel therapeutic candidate for the treatment of SCC.

研究范围本研究旨在探讨α-酮在鳞状细胞癌（SCC）中的抗癌特性：通过 RT-PCR 和 qRT-PCR 分析，证明 OR10A6 和嗅觉受体信号成分在 A431 人 SCC 细胞中的表达。使用配体α-酮激活A431细胞中的OR10A6可抑制细胞增殖和迁移，但会诱导细胞凋亡。其机制涉及 Hippo 通路的核心蛋白，其中大肿瘤抑制激酶（LATS）、yes 相关蛋白（YAP）和具有 PDZ 结合基调的转录辅激活因子（TAZ）的磷酸化已被 Western 印迹所证实。然而，在敲除 OR10A6 基因的 A431 细胞中，α-ionone 的抗癌作用会减弱。在 A431 异种移植小鼠模型中，注射 α-ionone 可抑制肿瘤生长、诱导细胞凋亡并增加 Hippo 通路中 LATS-YAP-TAZ 信号轴的磷酸化。在OR10A6基因敲除的异种移植肿瘤中没有观察到这些效应：这些发现共同表明，α-酮在SCC细胞中激活异位OR OR10A6可刺激Hippo通路并抑制体外和体内的肿瘤发生，为治疗SCC提供了一种新的候选疗法。

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引用次数: 0

Polar Lipids Supplementation Enhances Basal Excitatory Synaptic Transmission in Primary Cortical Neuron 补充极性脂质可增强初级皮层神经元的基础兴奋性突触传递

IF 4.5 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research

Pub Date : 2024-07-10 DOI: 10.1002/mnfr.202300883

Xin Yi Yeo, Dao Tam, Yunju Jo, Jung Eun Kim, Dongryeol Ryu, Jia Pei Chan, Sangyong Jung

Scope

Polar lipids, such as gangliosides and phospholipids, are fundamental structural components that play critical roles in the development and maturation of neurons in the brain. Recent evidence has demonstrated that dietary intakes of polar lipids in early life are associated with improved cognitive outcomes during infancy and adolescence. However, the specific mechanisms through which these lipids impact cognition remain unclear.

Methods and results

This study examines the direct physiological impact of polar lipid supplementation, in the form of buttermilk powder, on primary cortical neuron growth and maturation. The changes are measured with postsynaptic current response recordings, immunohistochemical examination of functional synapse localization and numbers, and the biochemical quantification of receptors responsible for neuronal synaptic neurotransmission. Chronic exposure to polar lipids increases primary mouse cortical neuron basal excitatory synapse response strength attributed to enhanced dendritic complexity and an altered expression of the excitatory α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunit 2 (GluR2).

Conclusion

The present finding suggests that dietary polar lipids improve human cognition through an enhancement of neuronal maturation and/or function.

范围神经节苷脂和磷脂等极性脂质是大脑神经元发育和成熟过程中起关键作用的基本结构成分。最近的证据表明，早期膳食中极性脂质的摄入量与婴儿期和青春期认知能力的提高有关。然而，这些脂质影响认知的具体机制仍不清楚：本研究探讨了以酪乳粉形式补充极性脂质对初级皮层神经元生长和成熟的直接生理影响。这些变化是通过突触后电流反应记录、功能性突触定位和数量的免疫组化检查以及负责神经元突触神经传递的受体的生化定量来测量的。长期暴露于极性脂类会增加小鼠皮层神经元的基础兴奋性突触反应强度，这归因于树突复杂性的增强和兴奋性α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）受体亚基2（GluR2）表达的改变：本研究结果表明，膳食中的极性脂质可通过提高神经元的成熟度和/或功能来改善人类的认知能力。

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引用次数: 0