Sbornik vedeckych praci Lekarske fakulty Karlovy university v Hradci Kralove最新文献

Acetylcholinesterase (blood, hippocampus, frontal cortex, basal ganglia, septum and diaphragm) or butyrylcholinesterase (liver) activities following i.m. administration of tacrine (9-amino-1,2,3,4-tetrahydroacridine), its 7-methoxy- and 7-hydroxy derivatives to rats in dose of 1.2 x LD50 were detected. The most marked inhibition of the enzymes studied following tacrine administration was demonstrated in the frontal cortex, diaphragm, liver and blood. Inhibition of acetylcholinesterase in the frontal cortex and blood only was observed following administration of tacrine derivatives. The results indicate that inhibition of cholinesterases could be important but not unique part explaining the action of these drugs in general.

Repeated measurements of dust concentrations and work loads were made at the workplaces in iron foundries (interval 10 years) and processing of asbestos (dusts 27 years and work load 8 years). The work load was mild to moderate, rarely heavy (energy output 2.0 to 5.7 MJ in the shift) with a mean heart rate 94-106. The incidence of occupational diseases was stabilized in the foundries, increased in the asset processing plants (mostly lung cancer). Two thirds of the workers were smokers, in spite of that the workers in the foundries had their mean flow-volume curve almost identical with the standards. Their age conditioned diminution was faster especially for FEF50 and 25 and accompanied with a bigger rise of FRC and RV. Exercise testing demonstrated the same fitness as in normal population (2.7 +/- 0.7 l/min). As far as asbestos processing is concerned, a diminution of dust concentrations below the limits was found. Despite of this, an increase in lung cancer incidence was observed probably as a consequence of high dust concentrations in the past and smoking habits (2/3 of workers). The flow-volume curves were obviously smaller than the normals. With the rising of exposure to asbestos and with the advancing age (more close correlation), the deterioration of respiratory parameters (measured during 6-7 years) was two times faster. The correlations were more close for women then for men. In the man group, this deterioration was greater in the last measurements. The results of ergometer testing up to anaerobic threshold were identical with those found in normal population.

The localization of dipeptidylpeptidase IV (DPP IV) activity was studied at light microscope level in the mucosa, glands and capillary endothelium of the human foetuses and rat, mouse and guinea pig in some prenatal and postnatal terms. Alkaline phosphatase reaction was used for complete histochemical demonstration of capillary endothelium. The two enzymes were mapped by Lojda's methods (12). The study brings knowledge of the localization of DPP IV activity in the nasal cavity structures and gives a different time survey of the onset of DPP IV activity during ontogenesis. Considering this activity we can presume that this membrane-bound protease DPP IV may participate in the metabolism of neuropeptides in the nasal cavity and play some role in immunological disturbances of patients with rhinitis.

Science is of international nature. The development of technical languages in the individual branches of science is connected with frequent borrowing of foreign-language lexical material which is mostly of Latin or Greek origin. Latin has had an effect on vocabulary, word formation and syntax. The existence of a foreign scientific language above a national one is the main cause of the frequent penetration of foreign and borrowed words into German. Greek and Latin represent the traditional language material to be used in medical terminology. In scientific communication, Latin and Greek terms and also equivalents in a native language are used.

Long-term results of operative treatment of cardiospasm and megaoesophagus in 111 patients are presented. There were 67 women and 44 men treated, age 4 to 65. The authors have for several years used their own, in this Department developed method of operation for cardiospasm, which joins Heller's operation with the reconstruction of the cardia to prevent reflux. In very advanced stages of the disease as megaoesophagus they use an original surgical procedure, which consists in connecting the thoracic segment of the oesophagus with the prepyloric part of the stomach by means of a pedicle jejunal graft. Clinical follow-up supported by x-ray examination and endoscopy has shown very good results of such treatment.

It was shown in experiments on mice that the radioprotective effects of adenosine monophosphate (AMP) or of a combination of Mg aspartate and AMP join with those induced by hypobaric hypoxia. The hypotensive effects of these drugs lead probably to hypoxia in radiosensitive tissues which acts additively with hypoxia elicited by way of respiration. Furthermore, Mg aspartate and AMP decrease the toxicity of high degrees of hypobaric hypoxia. These effects can be explained by the ability of adenosine liberated from AMP and of magnesium to increase brain and heart blood perfusion and/or to mitigate excessive sympathetic activity. The pharmacological effects of AMP and Mg aspartate are thus not only radioprotective but also energy preserving and protecting vitally important organs against hypoxia toxicity. These effects may be of favourable importance in hypoxic radiotherapy.

The effects of i. v. administration of Na2H2EDTA and a novel chelator MgNa2EDTA (both drugs in doses of 0.05 and 0.1 mg. kg-1) on the left ventricular dP/dtmax. of the paced and non-paced heart were investigated in rabbits in vivo. Both chelators induced a decrease in cardiac contractility which was, especially after the lower doses of the drugs, significantly less pronounced after MgNa2EDTA (max. 69.6% in the non-paced and 75.2% in the paced heart) in comparison with Na2H2EDTA (max. 51.3% in the non-paced and 52.9% in the paced heart). The decrease in dP/dtmax. was similar both under the paced and non-paced heart conditions after administration of both doses of the drugs. On the basis of the results obtained, it is possible to assume that the negative inotropic effects of the new chelator MgNa2EDTA may be lower in comparison with those of Na2H2EDTA and that cardiac pacing does not influence the inotropic response to the chelators in a negative manner. Premature deaths of a number of animals after the administration of both chelators show a necessity of further studies concerning especially cardiac intracellular ion concentrations.

The present paper reviews the results from the study of spigots, i.e. cuticular spinning structures obtained by the scanning of 52 genera belonging to 28 families of araneomorph spiders. The finding of eight morphologically different forms of these structures being attached to ducts of the pyriform type of silk glands is of fundamental importance for the final conclusions. The comparative observations enable me to create a hypothesis dealing with the existence of several evolutionary lines of spiders, each of which is represented with their own form of spigots. Nevertheless the character of these spigots undoubtedly reflects phylogenetic relationships between the spider taxa. The detection of a special mechanoreceptive field on top of the anterolateral spinnerets increases the knowledge of the functional morphology of the spider's spinning apparatus.

The work is based on the following concepts: communication with patients ceases being a matter of experience learning only (the trial and error method) and should become a part of systematic training; it should not be included only into continuous education of graduated physicians but must be gradually introduced into undergraduate education as well; it is not an optional activity which may but may not be performed by physicians, but it is a specific component of their clinical competence. Today attempts to instruct medical students in communication with patients and their relatives are being made at many medical schools all over the world. Reports on such instruction are, however, scattered in various journals or in internal publications published by universities, faculties and clinics. Thus they are not available very easily. If there exist some survey works, they are mostly conceived on the national level. The objective of the study presented here is to show a comparatively comprehensive survey dealing with the medical student instruction on the international level, analyze trends in development and identify problems that have not been solved so far. The work consists of five chapters. The first one gives a short survey of current concepts in communication with patients reaching beyond the traditional training in taking medical history. Among others, the following factors are included here: explanation of both diagnostical and therapeutical methods to the patient, informing the patient about results obtained from the examination, telling him or her general conclusions and recommendations, informing patients of social associations of the disease, developing in the patients those attitudes which support their cooperation with physicians and the patient's will to live, ability to treat serious and terminal patients properly, ability to treat patients' relatives and communicate with other members of a medical team. The second chapter shows current attempts to train the medical student-patient communication in Czechoslovakia, especially at Charles University. Third chapter describes how medical students in the former USSR were instructed, especially at medical schools in Moscow. This kind of information is still difficult to be had for people from abroad. The fourth chapter is the most important. Current attempts to train medical students in communication with patients in Europe, North and South America, Asia, Africa and Australia are described there. Reference frames in which the instruction takes place (medical, educational, psychological frames) are studied.(ABSTRACT TRUNCATED AT 400 WORDS)

The commonest pathological findings in the upper gastrointestinal tract have been verified by studying 320 autopsies as related to chronic renal failure through the 20-year span. In our series of 99 patients having been hospitalized within the period of 1989 to 1990, lesions of upper gastrointestinal tract were summarized that might be responsible for bleeding in the course of the dialysis and after renal transplantation. In accordance with literature sources, the investigated group showed gastric and duodenal mucosal lesions to be the most frequent sources of hemorrhages. For both the early diagnosis and therapy all patients have to be examined prior to the initiation of a regular maintenance dialysis. Gastroscopy is also indicated in all patients without any exception. Subsequent conservative treatment should be performed in an intensive and accurate way. While unsuccessful, the surgery is indicated. Authors referred to indicatory criteria based on surgical management of gastroduodenal ulcer. Both the early diagnosis and indication to surgery were stated to be of crucial importance for patient's destiny when the conservative treatment has failed. The present study should contribute to a closer collaboration of nephrologists, gastroenterologists and surgeons in order to prevent such serious complications that are represented by gastrointestinal bleeding in patients with chronic renal failure.