S. Abdel-Raheem, G. Mohamed, H. Monzaly, M. Farghaly
This study was conducted to compare the effects of dietary eubiotics or intravenous amino acid infusions (IVAAI) as two different growth promoters on nutrient digestibility, rumen fermentation, performance, and blood biochemical parameters of buffalo calves in subtropical climatic conditions. Thirty male buffalo calves (284.40 ± 18.45 kg) were randomly distributed into three groups and fed a basal diet (BD) of concentrate feed mixture and roughages. The first group was fed BD and considered as the control, the second group was fed the BD supplemented with eubiotics at 1.0 kg/ton of concentrate, whereas the third group was intravenously infused with amino acid (IVAAI) injection at a dose of 2.0 ml/100 kg body weight. Results showed that the total gain and the average daily gain were improved (P < 0.05) with dietary eubiotics. The digestibility of some nutrients was increased (P < 0.05) with dietary addition of eubiotics. In addition, eubiotics stabilize (P < 0.05) the rumen pH, which reduce the risk of subacute ruminal acidosis but increased (P < 0.05) ruminal NH3-N and total volatile fatty acids. The rectal temperature was decreased (P < 0.05) with eubiotics supplementation. In conclusion, the use of eubiotics induced superior positive effects on the digestibility of nutrients, rumen fermentation, rumen enzymes, rumen protein concentration, growth performance, feed conversion, blood parameters and ameliorated the harmful effects of thermal stress of buffalo calves in comparison with intravenous infusion of amino acids. Key words: eubiotics; amino acids; buffalo calves; nutrient digestibility; rumen fermentation; growth performance
{"title":"THE EFFECTS OF DIETARY EUBIOTICS OR INTRAVENOUS AMINO ACID INFUSIONS ON NUTRIENT DIGESTIBILITY, RUMEN FERMENTATION, PERFORMANCE AND BLOOD PARAMETERS OF BUFFALO CALVES UNDER SUBTROPICAL CLIMATIC CONDITIONS","authors":"S. Abdel-Raheem, G. Mohamed, H. Monzaly, M. Farghaly","doi":"10.26873/svr-1588-2022","DOIUrl":"https://doi.org/10.26873/svr-1588-2022","url":null,"abstract":"This study was conducted to compare the effects of dietary eubiotics or intravenous amino acid infusions (IVAAI) as two different growth promoters on nutrient digestibility, rumen fermentation, performance, and blood biochemical parameters of buffalo calves in subtropical climatic conditions. Thirty male buffalo calves (284.40 ± 18.45 kg) were randomly distributed into three groups and fed a basal diet (BD) of concentrate feed mixture and roughages. The first group was fed BD and considered as the control, the second group was fed the BD supplemented with eubiotics at 1.0 kg/ton of concentrate, whereas the third group was intravenously infused with amino acid (IVAAI) injection at a dose of 2.0 ml/100 kg body weight. Results showed that the total gain and the average daily gain were improved (P < 0.05) with dietary eubiotics. The digestibility of some nutrients was increased (P < 0.05) with dietary addition of eubiotics. In addition, eubiotics stabilize (P < 0.05) the rumen pH, which reduce the risk of subacute ruminal acidosis but increased (P < 0.05) ruminal NH3-N and total volatile fatty acids. The rectal temperature was decreased (P < 0.05) with eubiotics supplementation. In conclusion, the use of eubiotics induced superior positive effects on the digestibility of nutrients, rumen fermentation, rumen enzymes, rumen protein concentration, growth performance, feed conversion, blood parameters and ameliorated the harmful effects of thermal stress of buffalo calves in comparison with intravenous infusion of amino acids.\u0000Key words: eubiotics; amino acids; buffalo calves; nutrient digestibility; rumen fermentation; growth performance","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48643514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Nassar, Yasmin Hassan Bayumi, H. Selim, Shimaa A. Ezzeldein
This study was conducted to evaluate and to compare the clinical, hemato-biochemical and endoscopic aspects of gastropathy in dogs treated with meloxicam alone or incombination with esomeprazole and misoprostol. Twenty baladi healthy dogs were included in the experimental study. Dogs were divided into four groups each group consisting of five animals; Group I (control group), the group that does not receive any medication. Meloxicam treated groups divided into: Group II which received meloxicam at a dose of 0.2 mg/kg BWT per OS /24 hr. Group III animals received the same previous dose of meloxicam and esomeprazole at a dose of 1mg/kg BWT per OS /24 hr. Group IV animals received the same dose of meloxicam and misoprostol 3μg /kg BWT per OS tid. Upon drug administration, dogs were kept under observation for 14 consecutive days. Clinical and hemato-biochemical analysis were evaluated across time (T0, T3, T7, T10 and T14). The image analysis of the gastroscopic examination was evaluated across time (T0, T7 and T14), endoscopic examinations were applied to all animals in four groups at three time points (T0, T7, and T14), endoscopic lesions were scored by use of a 5-point scale. Clinically, the most common clinical sings in dogs with Meloxicam induced- gastropathy were inappetence to anorexia, hematemesis, melena, abdominal pain and weakness, the specific endoscopic lesions of gastropathy were gastric erosion, hemorrhage and ulcers. Serum gastrin concentration is a biochemically sensitive indicator of gastropathy. The overall results concluded that meloxicam-induced gastropathy was more severe in group II compared to groups III and group IV. The proton pump inhibitor (esomeprazole) was more effective and better tolerated than misoprostol.
{"title":"MELOXICAM-INDUCED GASTROPATHY IN DOGS: CLINICAL, BIOCHEMICAL, ENDOSCOPIC FEATURES AND TRIALS FOR PREVENTION","authors":"G. Nassar, Yasmin Hassan Bayumi, H. Selim, Shimaa A. Ezzeldein","doi":"10.26873/svr-1571-2022","DOIUrl":"https://doi.org/10.26873/svr-1571-2022","url":null,"abstract":"This study was conducted to evaluate and to compare the clinical, hemato-biochemical and endoscopic aspects of gastropathy in dogs treated with meloxicam alone or incombination with esomeprazole and misoprostol. Twenty baladi healthy dogs were included in the experimental study. Dogs were divided into four groups each group consisting of five animals; Group I (control group), the group that does not receive any medication. Meloxicam treated groups divided into: Group II which received meloxicam at a dose of 0.2 mg/kg BWT per OS /24 hr. Group III animals received the same previous dose of meloxicam and esomeprazole at a dose of 1mg/kg BWT per OS /24 hr. Group IV animals received the same dose of meloxicam and misoprostol 3μg /kg BWT per OS tid. Upon drug administration, dogs were kept under observation for 14 consecutive days. Clinical and hemato-biochemical analysis were evaluated across time (T0, T3, T7, T10 and T14). The image analysis of the gastroscopic examination was evaluated across time (T0, T7 and T14), endoscopic examinations were applied to all animals in four groups at three time points (T0, T7, and T14), endoscopic lesions were scored by use of a 5-point scale. Clinically, the most common clinical sings in dogs with Meloxicam induced- gastropathy were inappetence to anorexia, hematemesis, melena, abdominal pain and weakness, the specific endoscopic lesions of gastropathy were gastric erosion, hemorrhage and ulcers. Serum gastrin concentration is a biochemically sensitive indicator of gastropathy. The overall results concluded that meloxicam-induced gastropathy was more severe in group II compared to groups III and group IV. The proton pump inhibitor (esomeprazole) was more effective and better tolerated than misoprostol. ","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46254488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Liver fibrosis still life-threatening problem and searching out ameliorative products motives many scientists, so this work evaluated the anti-fibrotic and antioxidant role of naringenin (NAR) against thioacetamide (TAA) induced liver fibrosis. Fifty adult male albino rats randomly divided into 5 groups (10 each); the first kept as control; the second treated I/P by 200 mg/kg TAA twice a week for 8 weeks; the third was gavaged daily with 50 mg /kg/ b.wt of NAR for 8 weeks; the fourth was co-treated by TAA and NAR while the fifth was treated with TAA for 8 w then gavaged daily by NAR for 1 month. TAA administration significantly increases the hepatic cell enzymes (ALT, AST, ALP and GGT) in the serum referring to hepatic cell destruction with an increase in hepatic MDA with a reduction in GSH concentrations, antioxidant enzyme activities as well as down regulation of their expression levels. NAR administration either with or after TAA ameliorates this effect suggesting its antioxidant ability. In the fibrotic pathway, TAA treatment up-regulates the expression levels of fibrogenic biomarkers (TGF-β, collagen 1α and fibronectin) genes while NAR down-regulates these genes suggesting its anti-fibrotic ability. Histopathological analysis confirms the biochemical results. In conclusion, NAR ameliorates the deleterious effect of TAA through its antioxidant and anti-fibrotic abilities.
{"title":"ANTI-FIBROTIC AND ANTIOXIDANT AMELIORATIVE EFFECTS OF NARINGENIN AGAINST THIOACETAMIDE INDUCED LIVER FIBROSIS","authors":"H. A. Ali, Mohamed Afifi","doi":"10.26873/svr-1598-2022","DOIUrl":"https://doi.org/10.26873/svr-1598-2022","url":null,"abstract":"Liver fibrosis still life-threatening problem and searching out ameliorative products motives many scientists, so this work evaluated the anti-fibrotic and antioxidant role of naringenin (NAR) against thioacetamide (TAA) induced liver fibrosis. Fifty adult male albino rats randomly divided into 5 groups (10 each); the first kept as control; the second treated I/P by 200 mg/kg TAA twice a week for 8 weeks; the third was gavaged daily with 50 mg /kg/ b.wt of NAR for 8 weeks; the fourth was co-treated by TAA and NAR while the fifth was treated with TAA for 8 w then gavaged daily by NAR for 1 month. TAA administration significantly increases the hepatic cell enzymes (ALT, AST, ALP and GGT) in the serum referring to hepatic cell destruction with an increase in hepatic MDA with a reduction in GSH concentrations, antioxidant enzyme activities as well as down regulation of their expression levels. NAR administration either with or after TAA ameliorates this effect suggesting its antioxidant ability. In the fibrotic pathway, TAA treatment up-regulates the expression levels of fibrogenic biomarkers (TGF-β, collagen 1α and fibronectin) genes while NAR down-regulates these genes suggesting its anti-fibrotic ability. Histopathological analysis confirms the biochemical results. In conclusion, NAR ameliorates the deleterious effect of TAA through its antioxidant and anti-fibrotic abilities.","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46763217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cattle play an important role in the food chain as a major milk and meat producer. Helminths affect the health and production of livestock which need to be controlled efficiently and economically. The high usage of anthelmintic drugs has increased anthelmintic resistance to all available classes resulting in failed treatment options and economic losses. Herbs, which may be better options for animal and food safety, are among the best candidates to control these parasites. For this purpose, a research experiment was conducted. Twelve Sahiwal heifers were used for the evaluation of the anthelmintic activity of herbal dewormers composed of various parts of local herbs. Animals were divided into 4 groups A, B, C and D, all the groups, except group D were given herbal dewormer powder. In an experiment of 30 days, doses were given on a weekly basis. Fecal samples for egg counts, blood and serum samples were obtained fortnightly and subjected to statistical analysis. Results revealed that the animals receiving herbal dewormer @ 1400 mg/Kg had the best results and egg counts and hematological values were significantly (P<0.05) better than the control animals. The results of the study suggested that the herbal dewormer was efficient in reducing the worm counts as well as beneficial for the hematological profile of the cattle.
{"title":"EVALUATION OF ANTHELMINTIC, HEMATOLOGICAL, AND SERUM BIOCHEMICAL EFFECTS OF HERBAL DEWORMER ON THE CATTLE","authors":"Abdullah F. Alsayeqh, Zohaib Saeed","doi":"10.26873/svr-1624-2022","DOIUrl":"https://doi.org/10.26873/svr-1624-2022","url":null,"abstract":"Cattle play an important role in the food chain as a major milk and meat producer. Helminths affect the health and production of livestock which need to be controlled efficiently and economically. The high usage of anthelmintic drugs has increased anthelmintic resistance to all available classes resulting in failed treatment options and economic losses. Herbs, which may be better options for animal and food safety, are among the best candidates to control these parasites. For this purpose, a research experiment was conducted. Twelve Sahiwal heifers were used for the evaluation of the anthelmintic activity of herbal dewormers composed of various parts of local herbs. Animals were divided into 4 groups A, B, C and D, all the groups, except group D were given herbal dewormer powder. In an experiment of 30 days, doses were given on a weekly basis. Fecal samples for egg counts, blood and serum samples were obtained fortnightly and subjected to statistical analysis. Results revealed that the animals receiving herbal dewormer @ 1400 mg/Kg had the best results and egg counts and hematological values were significantly (P<0.05) better than the control animals. The results of the study suggested that the herbal dewormer was efficient in reducing the worm counts as well as beneficial for the hematological profile of the cattle.","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43235887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shimaa Mohamed Abdullah, Abdel Khalek Ramadan El-Sheikh, A. Mahmoud, N. E. Attia
Fever, dullness, tachypnea, bilateral nasal discharge, cough, dyspnea, and abnormal lung auscultation were all symptoms of pneumonic goats. To evaluate the hematological, biochemical and pulmonary function changes in 45 pneumonic goats compared with 10 apparently healthy ones, whole blood and serum samples were collected. The results revealed that pneumonic goats had significantly lower (P<0.05) RBCs, Hb, and PCV levels than healthy goats. A decrease in lymphocyte count and an increase in WBCs and neutrophils were found to be significant (P<0.05) in pneumonic goats compared to healthy ones. Blood pH and pO2 were significantly reduced (P<0.05) while pCO2, tCO2, and HCO3 were significantly elevated (P<0.05) in pneumonic goats compared to healthy ones. Biochemically, K, MDA, Hp and SAA showed significant (P<0.05) increase, whereas Cl and TAC showed a significant (P<0.05) decrease in the pneumonic goats compared to control group. The echotexture of the pneumonic consolidation on ultrasound resembles that of the liver. The chest x-ray showed increased opacity with a cotton-wool-like look that may be more grey or white. According to the findings, the diagnostic techniques mentioned above are helpful in diagnosing goat pneumonia. So, this study aimed to evaluate some selected biochemical parameters and different diagnostic methods including ultrasonography and radiography in pneumonic goats.
{"title":"CLINICAL, HEMATOBIOCHEMICAL AND RADIOGRAPHICAL STUDIES OF CAPRINE PNEUMONIA","authors":"Shimaa Mohamed Abdullah, Abdel Khalek Ramadan El-Sheikh, A. Mahmoud, N. E. Attia","doi":"10.26873/svr-1562-2022","DOIUrl":"https://doi.org/10.26873/svr-1562-2022","url":null,"abstract":"Fever, dullness, tachypnea, bilateral nasal discharge, cough, dyspnea, and abnormal lung auscultation were all symptoms of pneumonic goats. To evaluate the hematological, biochemical and pulmonary function changes in 45 pneumonic goats compared with 10 apparently healthy ones, whole blood and serum samples were collected. The results revealed that pneumonic goats had significantly lower (P<0.05) RBCs, Hb, and PCV levels than healthy goats. A decrease in lymphocyte count and an increase in WBCs and neutrophils were found to be significant (P<0.05) in pneumonic goats compared to healthy ones. Blood pH and pO2 were significantly reduced (P<0.05) while pCO2, tCO2, and HCO3 were significantly elevated (P<0.05) in pneumonic goats compared to healthy ones. Biochemically, K, MDA, Hp and SAA showed significant (P<0.05) increase, whereas Cl and TAC showed a significant (P<0.05) decrease in the pneumonic goats compared to control group. The echotexture of the pneumonic consolidation on ultrasound resembles that of the liver. The chest x-ray showed increased opacity with a cotton-wool-like look that may be more grey or white. According to the findings, the diagnostic techniques mentioned above are helpful in diagnosing goat pneumonia. So, this study aimed to evaluate some selected biochemical parameters and different diagnostic methods including ultrasonography and radiography in pneumonic goats.","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42605099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Isayed I. Hassanein, A. Metwally, H. E. M. Abd Elbaky
This study examined the effects of nucleotide supplementation on broiler chick growth performance, immunological response, carcass traits, meat quality, serum biochemical parameters, total antioxidant capacity, intestinal morphology, mortality rate, and economic efficiency measures of broiler chicks. A total of 180 one-day-old chicks (Ross 308) were distributed into 6 groups, each of which consisted of 30 chicks, and each group was divided into 3 replicates so that each replicate contained 10 chicks fed on six experimental diets as follows: basal diet without oil or nucleotide (T1), basal diet with 1% soybean oil and without nucleotide(T2), 50 grams nucleotide per 100-kilogram diet with different fat sources (no oil(T3), 1% soybean oil(T4), 1% linseed oil(T5) &1% fish oil(T6) respectively during the experimental period (5 weeks). Growth performance parameters were detected per stage period. Four chicks from each replicate were used at the termination of the experiment for analysis of the above mentioned measurements. Results revealed that supplementation of nucleotide in diets of broiler chicks improved feed conversion ratio, carcass traits, intestinal morphology, serum biochemical parameters, immunological response, bursa of Fabricius weight, and the best ratios were observed in groups fed nucleotide in combination with fish and linseed oil. Also, there was an increased economic efficiency in the SBO fed group(T2) then group fed nucleotides in mix with linseed oil (T5) and control (T1). In comparison to the control groups (T1 & T2), groups fed nucleotides and PUFA oil sources had significantly lower n-6: n-3 ratio in breast muscle, and mortality rate.
{"title":"EFFECTS OF DIETARY NUCLEOTIDES SUPPLEMENTATION ON THE PERFOR-MANCE OF BROILER CHICKS","authors":"Isayed I. Hassanein, A. Metwally, H. E. M. Abd Elbaky","doi":"10.26873/svr-1575-2022","DOIUrl":"https://doi.org/10.26873/svr-1575-2022","url":null,"abstract":"This study examined the effects of nucleotide supplementation on broiler chick growth performance, immunological response, carcass traits, meat quality, serum biochemical parameters, total antioxidant capacity, intestinal morphology, mortality rate, and economic efficiency measures of broiler chicks. A total of 180 one-day-old chicks (Ross 308) were distributed into 6 groups, each of which consisted of 30 chicks, and each group was divided into 3 replicates so that each replicate contained 10 chicks fed on six experimental diets as follows: basal diet without oil or nucleotide (T1), basal diet with 1% soybean oil and without nucleotide(T2), 50 grams nucleotide per 100-kilogram diet with different fat sources (no oil(T3), 1% soybean oil(T4), 1% linseed oil(T5) &1% fish oil(T6) respectively during the experimental period (5 weeks). Growth performance parameters were detected per stage period. Four chicks from each replicate were used at the termination of the experiment for analysis of the above mentioned measurements. Results revealed that supplementation of nucleotide in diets of broiler chicks improved feed conversion ratio, carcass traits, intestinal morphology, serum biochemical parameters, immunological response, bursa of Fabricius weight, and the best ratios were observed in groups fed nucleotide in combination with fish and linseed oil. Also, there was an increased economic efficiency in the SBO fed group(T2) then group fed nucleotides in mix with linseed oil (T5) and control (T1). In comparison to the control groups (T1 & T2), groups fed nucleotides and PUFA oil sources had significantly lower n-6: n-3 ratio in breast muscle, and mortality rate.","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42680156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Morshdy, A. Alsayeqh, Mohammed F. Aljasir, Hassan Mohieldeen, Shymaa Gamal El-Abody, Mohamed Elsayed Mohamed, W. Darwish
Rabbit meat and offal are considered valuable sources of high biological value animal protein. Rabbit meat is rich in essential amino acids, low in cholesterol, and contains considerable amounts of trace elements such as calcium, magnesium, and zinc. However, the potential contribution of rabbit meat and offal in the transmission of foodborne pathogens such as Staphylococcus aureus (S. aureus) and Salmonella spp. is neglected. Therefore, this study was conducted first to investigate the prevalence rates of S. aureus and Salmonella spp. in the retailed rabbit meat at Sharkia Governorate, Egypt. Second, serological identification of the isolated bacteria was followed. Detection of S. aureus-enterotoxins and Salmonella-virulence-associated genes was also done using PCR. Antimicrobial susceptibility testing of the recovered bacterial isolates was additionally examined. The acquired results showed that 17% of the investigated samples of rabbit meat contained S. aureus. Where S. aureus was isolated from the investigated rabbit's breast, thigh, liver, and kidney at 20%, 24%, 12%, and 12% of each, respectively. Salmonella spp. was isolated at 13%. Salmonella spp. was isolated from the investigated rabbit thigh, liver, breast, and kidney at 16%, 16%, 12%, and 8%, respectively. Four different strains of Salmonella spp namely, S. Typhimurium, S. Kentucky, S. Virchow, and S. Infantis were recovered in the current study. The recovered S. aureus and Salmonella spp. harbored enterotoxins and virulence attributes with multidrug resistance. Therefore, strict hygienic measures should be followed during the processing and handling of rabbit meat and offal.
{"title":"RABBIT MEAT AS A POTENTIAL SOURCE OF STAPHYLOCOCCUS AUREUS AND SALMONELLA SPP.","authors":"A. Morshdy, A. Alsayeqh, Mohammed F. Aljasir, Hassan Mohieldeen, Shymaa Gamal El-Abody, Mohamed Elsayed Mohamed, W. Darwish","doi":"10.26873/svr-1674-2023","DOIUrl":"https://doi.org/10.26873/svr-1674-2023","url":null,"abstract":"Rabbit meat and offal are considered valuable sources of high biological value animal protein. Rabbit meat is rich in essential amino acids, low in cholesterol, and contains considerable amounts of trace elements such as calcium, magnesium, and zinc. However, the potential contribution of rabbit meat and offal in the transmission of foodborne pathogens such as Staphylococcus aureus (S. aureus) and Salmonella spp. is neglected. Therefore, this study was conducted first to investigate the prevalence rates of S. aureus and Salmonella spp. in the retailed rabbit meat at Sharkia Governorate, Egypt. Second, serological identification of the isolated bacteria was followed. Detection of S. aureus-enterotoxins and Salmonella-virulence-associated genes was also done using PCR. Antimicrobial susceptibility testing of the recovered bacterial isolates was additionally examined. The acquired results showed that 17% of the investigated samples of rabbit meat contained S. aureus. Where S. aureus was isolated from the investigated rabbit's breast, thigh, liver, and kidney at 20%, 24%, 12%, and 12% of each, respectively. Salmonella spp. was isolated at 13%. Salmonella spp. was isolated from the investigated rabbit thigh, liver, breast, and kidney at 16%, 16%, 12%, and 8%, respectively. Four different strains of Salmonella spp namely, S. Typhimurium, S. Kentucky, S. Virchow, and S. Infantis were recovered in the current study. The recovered S. aureus and Salmonella spp. harbored enterotoxins and virulence attributes with multidrug resistance. Therefore, strict hygienic measures should be followed during the processing and handling of rabbit meat and offal.\u0000 ","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47327506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Histamine is the biogenic amine responsible for scombroid poisoning in various types of fish and fish products. The study evaluated histamine content and bacteriological quality of salted and freeze sardine samples. All fish samples were cultured on Reinforced Closterdial medium; mannitol salt (MS) agar and violet red bile glucose (VRBG) agar, the positive colonies were sub-cultured on histamine-forming bacterium agar, followed by biochemical and PCR identification. Production of histamine in the fish muscles was measured by high performance liquid chromatography. The mean histamine values were about; 8.25 mg/100g. The mean of total aerobic counts was; 4×104 ± 2.1×104 while, the total anaerobic count; 5×104 ±1.2×103 Log CFU/g. Enterobacteriacea spp. about; 5×104 ±2.5×104 Log CFU/g while, the mean value of Staphylococcus spp. about; 3×104 ±3.7×104 Log CFU/g. The mean value of histamine-forming bacterial were; 3.3×106 Log CFU/g. Molecular Identification For Enterobacteriaceae were Klebsiella pneumonia, Staphylococcus xylosus, Escherichia coli and Enterobacter cloacae. We concluded that during storage, there was a positive relationship between the histamine levels and the histamine-forming bacteria (HFB) numbers, while, histamine and the bacteria forming histamine were recorded in all examined sardine samples. Key words: histamine; Escherichia coli; Klebsiella pneumonia; molecular identification; scombroid; Staphylococcus xylosus
{"title":"HISTAMINE LEVEL AND HISTAMINE-PRODUCING BACTERIA ISOLATED FROM SALTED AND FREEZE SARDINE FISH (Sardina spp.)","authors":"A. Elazzazy, M. Mahmoud, A. Al-Hejin, A. Ahmed","doi":"10.26873/svr-1633-2022","DOIUrl":"https://doi.org/10.26873/svr-1633-2022","url":null,"abstract":"Histamine is the biogenic amine responsible for scombroid poisoning in various types of fish and fish products. The study evaluated histamine content and bacteriological quality of salted and freeze sardine samples. All fish samples were cultured on Reinforced Closterdial medium; mannitol salt (MS) agar and violet red bile glucose (VRBG) agar, the positive colonies were sub-cultured on histamine-forming bacterium agar, followed by biochemical and PCR identification. Production of histamine in the fish muscles was measured by high performance liquid chromatography. The mean histamine values were about; 8.25 mg/100g. The mean of total aerobic counts was; 4×104 ± 2.1×104 while, the total anaerobic count; 5×104 ±1.2×103 Log CFU/g. Enterobacteriacea spp. about; 5×104 ±2.5×104 Log CFU/g while, the mean value of Staphylococcus spp. about; 3×104 ±3.7×104 Log CFU/g. The mean value of histamine-forming bacterial were; 3.3×106 Log CFU/g. Molecular Identification For Enterobacteriaceae were Klebsiella pneumonia, Staphylococcus xylosus, Escherichia coli and Enterobacter cloacae. We concluded that during storage, there was a positive relationship between the histamine levels and the histamine-forming bacteria (HFB) numbers, while, histamine and the bacteria forming histamine were recorded in all examined sardine samples.\u0000Key words: histamine; Escherichia coli; Klebsiella pneumonia; molecular identification; scombroid; Staphylococcus xylosus","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48949390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jin-Kui Ma, A. Alsayeqh, W. El-Ghareeb, A. Elhelaly, M. Seliem, W. Darwish, Karima Mohamed Eissa Abdallah
The consumption of exotic meats, such as squab and quail meats, is common in many parts of the world. However, little is known about the safety of these foods. This study aimed to evaluate the microbial characteristics of squab and quail meats, with a particular interest in the prevalence of Shiga toxin-producing Escherichia coli (STEC). Meat samples were examined for total bacterial counts, total mold counts, most probable number of coliforms, total E. coli counts, and the prevalence of STEC. The presence of virulence genes (stx1, stx2, and eae) in STEC isolates was also investigated. Results from microbial analyses revealed poor hygienic status of squab and quail meats. E. coli was isolated from 16% and 10% of squab and quail meats, respectively. Six E. coli serotypes were recovered, including O26, O78, O111, O114, O119, and O127, and STEC genes were detected in all these isolates. Squab liver had the highest E. coli prevalence rates, followed by gizzard, heart, spleen, and breast muscles. The prevalence of E. coli in quail meat samples was similar across all tissues. STEC serotypes showed notable multidrug resistance profiles. We then used ascorbic and rosmarinic acids to increase the safety of breast muscle. Treatment of breast muscles with these acids significantly improved their microbial safety. These findings highlight the potential role of squab and quail meats as a vehicle for STEC transmission to humans, and the beneficial effect of treatment with ascorbic and rosmarinic acids on enhancing the safety of exotic meats. Key words: food safety; shiga toxin; E. coli; squab; quails; meat
{"title":"SQUAB AND QUAIL MEATS: MICROBIAL STATUS AND PREVALENCE OF MULTIDRUG-RESISTANT SHIGA TOXIN-PRODUCING E. coli","authors":"Jin-Kui Ma, A. Alsayeqh, W. El-Ghareeb, A. Elhelaly, M. Seliem, W. Darwish, Karima Mohamed Eissa Abdallah","doi":"10.26873/svr-1613-2022","DOIUrl":"https://doi.org/10.26873/svr-1613-2022","url":null,"abstract":"The consumption of exotic meats, such as squab and quail meats, is common in many parts of the world. However, little is known about the safety of these foods. This study aimed to evaluate the microbial characteristics of squab and quail meats, with a particular interest in the prevalence of Shiga toxin-producing Escherichia coli (STEC). Meat samples were examined for total bacterial counts, total mold counts, most probable number of coliforms, total E. coli counts, and the prevalence of STEC. The presence of virulence genes (stx1, stx2, and eae) in STEC isolates was also investigated. Results from microbial analyses revealed poor hygienic status of squab and quail meats. E. coli was isolated from 16% and 10% of squab and quail meats, respectively. Six E. coli serotypes were recovered, including O26, O78, O111, O114, O119, and O127, and STEC genes were detected in all these isolates. Squab liver had the highest E. coli prevalence rates, followed by gizzard, heart, spleen, and breast muscles. The prevalence of E. coli in quail meat samples was similar across all tissues. STEC serotypes showed notable multidrug resistance profiles. We then used ascorbic and rosmarinic acids to increase the safety of breast muscle. Treatment of breast muscles with these acids significantly improved their microbial safety. These findings highlight the potential role of squab and quail meats as a vehicle for STEC transmission to humans, and the beneficial effect of treatment with ascorbic and rosmarinic acids on enhancing the safety of exotic meats.\u0000Key words: food safety; shiga toxin; E. coli; squab; quails; meat","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42724587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Areej I. Salem, Hosny Abd El-Fadil, Nagaah Al-Sayed, Ahmed S. Alazzouni, S. El-Nabtity
Breast cancer is the most frequent type of invasive cancer in women. However, chemotherapy affects all cells that grow and divide quickly in the body, including cancer and normal cells. On the other hand, Graviola is commonly used as a source of food and has a wide range of bioactive components. In this study, out of 50 albino rats, breast cancer was induced in 40 rats using 7,12-dimethylbenanthracene (DMBA). These rats were subjected to the treatment using Graviola leaves ethanolic extract (GLEE) and 5-Fluorouracil (5-FU) to evaluate the anti-carcinogenic activity and the immunohistochemical changes. A single oral dose of DMBA led to the induction of rats’ breast cancer. Induced mammary tumors were diagnosed, and all were malignant without any benign tumor. Among the malignant tumors, non-invasive, invasive, mixed (invasive and non-invasive), and unclassified malignant tumors were detected. The immunohistochemical analysis showed that estrogen and progesterone receptors revealed negative nuclear expression. However, HER2 receptors score was +3, and Ki-67 revealed 85-90% nuclear stainability, denoting a very high proliferative index. The morphometric analysis revealed that staining reactivities of HER2 and Ki-67 were 60.66 and 89.84%, respectively, and were significantly higher than ER (15.24 %) and PR (15.68%). Genetic studies revealed marked upregulation of P53 with GLEE more than 5-FU while Bcl2 showed down regulation with GLEE more than 5-FU. The quantitative analysis of GLEE phytochemical constituents showed the presence of some bioactive chemical compounds that exhibit many therapeutic activities. Therefore, GLEE improved histological appearance of DMBA induced breast cancer in female rats. Therefore, GLEE could be a promising natural alternative to chemotherapy agents as a potent anticancer.
{"title":"ANTI-CANCER ACTIVITY OF GRAVIOLA (ANNONA MURICATA) LEAVES EXTRACT ON INDUCED BREAST CANCER IN RATS’ MODEL","authors":"Areej I. Salem, Hosny Abd El-Fadil, Nagaah Al-Sayed, Ahmed S. Alazzouni, S. El-Nabtity","doi":"10.26873/svr-1573-2022","DOIUrl":"https://doi.org/10.26873/svr-1573-2022","url":null,"abstract":"Breast cancer is the most frequent type of invasive cancer in women. However, chemotherapy affects all cells that grow and divide quickly in the body, including cancer and normal cells. On the other hand, Graviola is commonly used as a source of food and has a wide range of bioactive components. In this study, out of 50 albino rats, breast cancer was induced in 40 rats using 7,12-dimethylbenanthracene (DMBA). These rats were subjected to the treatment using Graviola leaves ethanolic extract (GLEE) and 5-Fluorouracil (5-FU) to evaluate the anti-carcinogenic activity and the immunohistochemical changes. A single oral dose of DMBA led to the induction of rats’ breast cancer. Induced mammary tumors were diagnosed, and all were malignant without any benign tumor. Among the malignant tumors, non-invasive, invasive, mixed (invasive and non-invasive), and unclassified malignant tumors were detected. The immunohistochemical analysis showed that estrogen and progesterone receptors revealed negative nuclear expression. However, HER2 receptors score was +3, and Ki-67 revealed 85-90% nuclear stainability, denoting a very high proliferative index. The morphometric analysis revealed that staining reactivities of HER2 and Ki-67 were 60.66 and 89.84%, respectively, and were significantly higher than ER (15.24 %) and PR (15.68%). Genetic studies revealed marked upregulation of P53 with GLEE more than 5-FU while Bcl2 showed down regulation with GLEE more than 5-FU. The quantitative analysis of GLEE phytochemical constituents showed the presence of some bioactive chemical compounds that exhibit many therapeutic activities. Therefore, GLEE improved histological appearance of DMBA induced breast cancer in female rats. Therefore, GLEE could be a promising natural alternative to chemotherapy agents as a potent anticancer.","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47149487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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