Slovenian Veterinary Research最新文献

{"title":"EFFICACY OF AMOXICILLIN (ATCOMOX®) AND/OR ALLICIN ON PERFORMANCE, HAEMATOLOGICAL, BIOCHEMICAL, AND HISTOPATHOLOGICAL CHANGES IN Clostridium perfringens INFECTED CHICKENS","authors":"M. Aboubakr, A. Elkomy, S. Belih, Mohamed Morad, Hassan Shaheen, Mohamed M. Abdel-Daim","doi":"10.26873/svr-867-2020","DOIUrl":"https://doi.org/10.26873/svr-867-2020","url":null,"abstract":"The efficacy of amoxicillin and/or allicinin healthy and experimentally Clostridium perfringens-infected broiler chickens was investigated. The chicks were equally divided into six groups, and all medications were orally administered via drinking water for five consecutive days: Group 1: non-infected and non-treated; Group 2: infected and non-treated; Group 3: infected and amoxicillin-treated (20 mg/kg b.wt); Group 4: infected and allicin-treated (25 mg/kg b.wt); Group 5: infected and treated with amoxicillin (20 mg/kg b.wt) and allicin (25 mg/kg b.wt); Group 6: infected and treated with amoxicillin (10 mg/kg b.wt) and allicin (25 mg/kg b.wt). Growth performance,haematological andbiochemical parameters were recorded. Significant decreases in total protein, albumin, RBCs, Hb, andPCV and a considerable increase in WBCs, AST, ALT, ALP, creatinine, anduric acid in infected chickens were observed. Administration amoxicillin and/or allicin for treatment of Clostridium perfringens infection resulted in improvement in haematological and biochemical changes following infection. A dose of amoxicillin (10 mg) and allicin (25 mg)/kg bwt for treatment of Clostridium perfringens infection in broiler chickens is recommended due to great synergistic effect, reduced mortality, greater safety, and increased economic potential. Key words: amoxicillin; allicin; efficacy; broilers; biochemical; hematological VPLIV AMOXICILINA (ATCOMOX®) IN ALICINA NA PRIRAST, HEMATOLOSKE, BIOKEMIJSKE IN HISTOPATOLOSKE SPREMEMBE PRI PISCANCIH, OKUŽENIH S Clostridium perfringens Povzetek: V studiji smo ugotavljali ucinkovitost amoksicilina in/ali alicinina pri zdravih pitovnih piscancih in pitovnih piscancih poskusno okuženimih z bakterijo Clostridium perfringens . Piscanci so bili razdeljeni v sest skupin in so zdravila dobivali peroralno preko vode pet dni zapored. V prvi skupini so bili neokuženi in nezdravljeni piscanci, v drugi okuženi in nezdravljeni, v tretji okuženi in zdravljeni z amoksicilinom (20 mg/kg telesne mase), v cetrti skupini okuženi in zdravljeni z alicinom (25 mg/kg telesne mase) v peti skupini okuženi in zdravljeni z amoksicilinom (20 mg/kg teže) in alicinom (25 mg/kg telesne mase) in v sesti skupini okuženi in zdravljeni z amoksicilinom (10 mg/kg teže) in alicinom (25 mg/kg telesne mase). Spremljali smo prirast piscancev ter njihove hematoloske in biokemicne parametre. Pri okuženih piscancih smo v krvi opazili znatno znižanje skupnih beljakovin, albuminov, RBC, Hb in PCV ter znatno povecanje WBC, AST, ALT, ALP, kreatinina in secne kisline. Uporaba amoksicilina in/ali alicina za zdravljenje okužbe s Clostridium perfringens je povzrocila izboljsanje hematoloskih in biokemicnih sprememb po okužbi. Odmerek amoksicilina 10 mg/kg in alicina 25 mg/kg telesne mase za zdravljenje okužbe s Clostridium perfringens pri pitovnih piscancih brojlerjih se je izkazal kot najbolj ucinkovit, verjetno zaradi sinergisticnega ucinka obeh zdravil, in je povzrocil zmanjsanje smrtnosti p","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2020-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43171890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CORTICAL BRANCHES OF THE MIDDLE CEREBRAL ARTERY IN EUROPEAN BADGER (Meles meles)","authors":"B. Skoczylas, W. Brudnicki, K. Kirkiłło-Stacewicz, W. Nowicki, J. Wach","doi":"10.26873/svr-549-2020","DOIUrl":"https://doi.org/10.26873/svr-549-2020","url":null,"abstract":"The pattern and variation of the cortical branches of the middle cerebral artery in European badger were studied for 64 cerebral hemispheres. It was found that the artery bifurcates into splits into ten permanent branches. Two olfactory arteries supply the area of the brain situated on the border between the old and the new cortex. The other eight branches get divided into three branches heading for the frontal region, two branches – to the parietal region and three temporal branches which supply only the new cortex. The frontal, parietal and temporal branches descended independently from the main trunk of the middle cerebral artery or formed a common trunk first. Common trunks for respective groups of branches have been described as the rostral, dorsal and caudal middle cerebral artery. The rostral olfactory artery in 6.3% of the cases investigated was an independent branch from the rostral cerebral artery.","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":"57 1","pages":"49-54"},"PeriodicalIF":0.1,"publicationDate":"2020-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44833419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COMPARISONS OF THE HISTOLOGICAL MORPHOLOGY AND IN VITRO PERCUTANEOUS ABSORPTION OF CAFFEINE IN SHED SNAKE SKIN AND HUMAN SKIN","authors":"M. Sacha, Nancy Weisbach, Anna-Sophie Pöhler, Nina Demmerle, E. Haltner","doi":"10.26873/svr-839-2020","DOIUrl":"https://doi.org/10.26873/svr-839-2020","url":null,"abstract":"The employment of excised skin (human or animal) mounted in diffusion cells is frequently used for the characterization of biopharmaceutical properties of topical semisolids dosage forms.  Reptile skin from snake appears to be a useful alternative to other animal and human skins in assessing the potential for transdermal drug delivery. The aim of the study was to compare human and snake skin from a histological point of view. Furthermore the absorption of caffeine, as a hydrophilic model substance, was compared on snake shed skins (two anatomical locations; ventral and dorsal), from three different species, Python regius , Epicrates maurus colombianus , Lampropeltis triangulum campbelli , and human skin.  Snake skin shows histological similarity to human Stratum corneum in term of thickness and composition. Regarding the absorption, the cumulative amount of caffeine increased linearly with time through the dorsal and ventral shed skins of all 3 species. Except for Lampropeltis triangulum campbelli ventral skin, the caffeine permeation behavior obtained on all snake shed skins evaluated was in a similar range as on human skin. One main advantage of shed skin, is that snakes molt regularly and can provide many sheds, that can be obtained without sacrificing the animals. Key words: caffeine; transdermal; shed skin; in vitro ; snake; topical formulation PRIMERJAVA HISTOLOSKE ZGRADBE TER PERKUTANE ABSORPCIJE KOFEINA IN VITRO V LEVKU KAC IN CLOVESKI KOŽI Povzetek: Koža (cloveskega ali živalskega izvora) se pogosto uporablja kot orodje za proucevanje biofarmacevtskih lastnosti topikalnih zdravil. Koža kac bi lahko bila uporabna alternativa drugim živalskim kožam in cloveski koži pri oceni sposobnosti prenosa zdravil preko kože. Cilj studije je bil histoloska primerjava cloveske in kacje kože ter primerjava absorpcije kofeina kot hidrofilne modelne snovi na levkih kac iz dorzalnega in ventralnega podrocja treh razlicnih vrst kac: kraljevega pitona ( Python regius ), mavricnega udava ( Epicrates maurus colombianus ), mlecne kace ( Lampropeltis triangulum campbelli ). Kacja koža je histolosko podobna cloveski glede debeline in sestave roženi plasti ( stratum corneum ). Glavna prednost levitve kac je, da se dogaja veckrat, kar omogoca pridobitev vec levkov, ki se jih lahko pridobi brez žrtvovanja živali. Rezultati so pokazali, da se je skupna kolicina kofeina scasoma linearno povecevala v dorzalnih in ventralnih levkih pri vseh treh vrstah kac, razen na na ventralnem podrocju levka mlecne kace ( Lampropeltis triangulum campbelli ), pri katerem je bila prehodnost kofeina podobna kot pri prehodu skozi clovesko kožo. Kljucne besede : kofein; transdermalno; levek; in vitro ; kaca; topikalni pripravek","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2020-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47126832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"REDUCING THE NUMBER OF BACTERIAL COLONIES USING ECOCID®S (POTASSIUM PEROXYSULPHATE BASED DISINFECTANT) AT SMALL ANIMAL CLINIC","authors":"N. Tozon, M. Biasizzo, L. Ščuka, T. Potočnik, M. Redek, Luka Prem","doi":"10.26873/svr-589-2020","DOIUrl":"https://doi.org/10.26873/svr-589-2020","url":null,"abstract":"A clinical study has been conducted to test the efficacy of Ecocid ® S, a biocidal agent. The active substance is potassium peroxysulphate and is used in clinical practice after the mechanical cleaning of various surfaces that act as potential sources of infection transmission. We determined 29 swabbing points, from which 87 samples were collected with cotton swabs. Swabs were submitted for microbiological testing to evaluate microbial contamination before cleaning, and before and after disinfection with Ecocid ® S. We submitted 63 swabs from 21 swabbing points for further statistical analysis. Five swabs were excluded because the presence of bacteria in the swabs before disinfection had not been determined. The clinical study on the efficacy of Ecocid ® S disinfectant showed that it is effective with an average reduction in contamination of 95.75%. The disinfectant was also active with a significantly reduced time of action: it was removed with dry paper towels from all sampling points, except the floor scales, only 5 to 10 minutes after application. The time required for the proper preparation of examination tables and other equipment in clinical practice is of vital importance for a smooth workflow. Key words: animals; disinfection; potassium peroxysulphate; Ecocid ® S ZMANJSANJE STEVILA BAKTERIJ PO UPORABI RAZKUŽILA ECOCID ® S (RAZKUŽILO NA OSNOVI KALIJEVEGA PEROKSISULFATA) V PROSTORIH KLINIKE ZA MALE ŽIVALI Povzetek: S klinicno studijo smo želeli ugotoviti ucinkovitosti biocidnega razkužila ECOCID®S. Aktivna snov v razkužilu je kalijev peroksisulfat, ki se na klinikah uporablja za razkuževanje razlicnih povrsin, ki predstavljajo kljucna mesta za prenos okužb, po njihovem mehanicnem ciscenju. Dolocili smo 29 vzorcnih mest, na katerih smo z uporabo bombažnih brisov odvzeli 87 vzorcev. Z mikrobioloskim testiranjem smo ugotavljali stopnjo kontaminacije pred ciscenjem, ter pred in po razkuževanju z ECOCID®S. Za statisticno obdelavo smo uporabili 63 rezultatov z 21 vzorcnih mest. Pet rezultatov je bilo izlocenih iz obdelave, ker je bila že pred razkuževanjem stopnja kontaminacije pod mejo detekcije uporabljene metode. S klinicno studijo ucinkovitosti razkužila Ecocid®S smo ugotovili povprecno 95,75 % zmanjsanje stevila kontaminantov po uporabi razkužila. Razkužilo je bilo ucinkovito tudi ob skrajsanem casu delovanja le 5 do 10 minut po nanosu. Zaradi zagotavljanja tekocega dela na kliniki je izredno pomemben cas, ki je potreben za ustrezno pripravo povrsin in druge opreme za pregled živali, zato je razkužilo Ecocid ®  S primerno za uporabo na veterinarskih klinikah, saj hitro in ucinkovito zmanjsa bakterijsko kontaminacijo. Kljucne besede: živali; dezinfekcija; kalijev peroksisulfat; Ecocid ®  S","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2020-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47267815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OCCURRENCE, MOLECULAR IDENTIFICATION AND ANTIBIOTIC RESISTANCE PROFILING OF Mycoplasma gallisepticum AND Mycoplasma synoviae FROM CHRONIC RESPIRATORY DISEASE CASES IN POULTRY BIRDS AND FARM ENVIRONMENT","authors":"A. Siddique, S. Rahman, M. Ulhaq, Rasheeha Naveed","doi":"10.26873/svr-598-2020","DOIUrl":"https://doi.org/10.26873/svr-598-2020","url":null,"abstract":"Avian mycoplasmosis is an important risk for commercial poultry production leading to enormous losses in terms of disease and productivity. The main causative agents are Mycoplasma gallisepticum and Mycoplasma synoviae. To study the variable degree of resistance to commonly prescribed and used antibiotics in mycoplasmosis, a total of 115 samples including tissue specimen and swabs were collected from chronic respiratory disease (CRD) cases of broiler and layer birds and their contaminated farm environment. The samples were directly passaged into the Brain Heart Infusion broth (supplemented with 10 % horse serum, NAD, cysteine, penicillin and thallium acetate). Positive samples were transferred to Brain Heart Infusion agar (Difco) for the isolation of Mycoplasma spp. while negative samples were declared after the third passage. Of the samples, 61.5% were found positive for Mycoplasma spp., which were recovered mostly after second passage. Out of total culture positive cases, Mycoplasma gallisepticum (MG) was identified in 62% cases and Mycoplasma synoviae (MS) in 38%, as confirmed through Polymerase Chain Reaction (PCR) using specific primers. The MG and MS isolates showed variable degrees of sensitivity against the commercially available drug of choice, tylosin. The highest Minimum Inhibitory Concentration (MIC) of enrofloxacin (112.38±4.34 μg/ml) was recorded against MG, followed by tetracyclin (91.58±4.66μl/ml), gentamicin (54.33±2.98 μg/ml), spiromicin (52.23±3.99 μg/ml) and tylosin (52.58±2.69 μg/ml). The highest MIC for enrofloxacin (168.24 ±3.82 μg/ml) was recorded against MS followed by tetracyclin (115.48±2.62 μg/ml), spiromicin (95.96 ±2.17 μg/ml), tylosin (84.84±2.56 μg/ml) and gentamicin (46.4±2.18 μg/ml). Multiplex PCR is a time tested tool for the molecular diagnosis and confirmation of Mycoplasma species.","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2020-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43778516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INVESTIGATION OF G+265C AND G-1539A SINGLE NUCLEOTIDE POLYMORPHISMS OF TOLL-LIKE RECEPTOR 4 GENE (TLR4) IN SOME CATTLE BREEDS RAISED IN TURKEY","authors":"Özgecan Korkmaz Ağaoğlu, B. Akyüz, Emel Zeytünlü, A. Ağaoğlu","doi":"10.26873/svr-914-2020","DOIUrl":"https://doi.org/10.26873/svr-914-2020","url":null,"abstract":"Toll-like receptors play an essential role in how the innate immune system reacts to pathogens. These receptors help the innate immune system recognise the antigenic structure of pathogens and initiate the inflammatory response. TLR4 is one of these receptors, and it has been identified as a candidate molecular marker for resistance to mastitis in cattle. This study aimed to describe G-1539A (BgII) in promoter and G+265C (MspI) in 5'-UTR polymorphisms in the TLR4 gene using Zavot (n=60), East Anatolian Red (EAR, n=49), Anatolian Black (AB, n=59), South Anatolian Red (SAR, n=42), Turkish Gray (TG, n=60) and Holstein (n=218) cattle breeds. For this purpose, we used polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) to genotype a total of 488 cattle. Two alleles (A and G) and three genotypes (GG, GA and AA) were examined by digestion of the PCR product with BgII restriction enzyme. The frequency of the G allele was higher in all investigated breeds except Zavot. The GA genotype was found to be the most common genotype in the EAR, SAR, TG, AB, Holstein, and Zavot breeds. Two alleles (C and G) and three genotypes (GG, GC and CC) were examined by digestion of the PCR product for the G+265C SNP with the MspI restriction enzyme. The most prevalent in terms of frequency was the C allele in all breeds with regard to G+265C SNP. All examined breeds were within the Hardy-Weinberg equilibrium (p>0.05). Consequently, the existence of two SNPs of the TLR4 gene has been reported for the first time in six cattle breeds raised in Turkey. The study demonstrated that the investigated breeds continue to exhibit variation in terms of these two SNPs. It might be possible to utilise these polymorphisms in efforts to breed herds resistant to significant breeding diseases, such as mastitis. Key words: cattle; PCR-RFLP; promoter; SNP; TLR4 RAZISKOVANJE ENOJNIH NUKLEOTIDNIH POLIMORFIZMOV G+265C IN G-1539A GENA RECEPTORJA TLR4 PRI NEKATERIH PASMAH KRAV, GOJENIH V TURCIJI Povzetek: Tolicni receptorji oz. receptorji TLR (iz angl. Toll-like receptors) igrajo bistveno vlogo pri reakciji prirojenega imunskega sistema na patogene. Ti receptorji pomagajo prirojenemu imunskemu sistemu prepoznati antigensko strukturo patogenov in sprožiti vnetni odziv. TLR4 je bil izmed skupine tolicnih receptorjev prepoznan kot kandidatni molekularni oznacevalec za odpornost na mastitis pri govedu. V studiji smo želeli opisati G-1539A (BgII) v promotorju in G+265C (MspI) v 5'-UTR polimorfizmih gena TLR4 pri razlicnih pasmah goveda: pasmi Zavot (n = 60), vzhodno anatolskem rdecem govedu (EAR, n = 49), anatolijskemu crnemu govedu (AB, n = 59), južno anatolskemu rdecemua govedu (SAR, n = 42), turskem sivem govedu (TG, n = 60) in govedu pasme Holstein (n = 218). V ta namen smo uporabili verižno reakcijo s polimerazo in polimorfizem dolžin restrikcijskih odsekov (PCR-RFLP) za genotipizacijo 488 govedi. Z restrikcijsko cepitvijo produktov PCR z restrikcijskim encimom BgII sm","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":"57 1","pages":"25-31"},"PeriodicalIF":0.1,"publicationDate":"2020-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47044369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GENOTYPIC CHARACTERIZATION OF A Trueperella pyogenes STRAIN AS A MAJOR CAUSATIVE AGENT OF METRITIS, ABORTION AND DEATH IN Bubalus bubalis","authors":"I. A. Tamai, A. Mohammadzadeh, P. Mahmoodi, T. Z. Salehi","doi":"10.26873/svr-515-2019","DOIUrl":"https://doi.org/10.26873/svr-515-2019","url":null,"abstract":"Trueperella pyogenes is a species of commensal bacteria which is present on the upper respiratory, urogenital and gastrointestinal mucosae of cattle. This species is able to cause pyogenic infections and health risks, alone or in association with other pyogenic bacteria. However, systemic disease with abortion and death in water buffalo has not yet been documented. Here, we isolated a strain of T. pyogenes , from a pregnant water buffalo ( Bubalus bubalis ) with metritis and pneumonia, which finally caused abortion and death in the affected host. Thereafter, the virulence genes and antibiotic resistance of the isolate were investigated. Single PCR method confirmed the presence of the well-known virulence genes of T. pyogenes including plo , nan H, nan P, cbp A, fim A, fim C, fim E and fim G genes. Antibiotic susceptibility test revealed that this isolate was resistant against Tetracycline, Erythromycin and Trimethoprim sulfamethoxazole. Furthermore, using Box-PCR method, it was determined that DNA fingerprint pattern of this isolate was different from that of a control strain ( T. pyogenes ATCC 19411). The results of the present study indicated that T. pyogenes can cause a systemic lethal disease in water buffalo. However, it seems that host and environmental conditions may also contribute to such infection. To our knowledge, this is the first report of a buffalo with pneumonia, metritis, abortion and death caused by T. pyogenes . Key words: Trueperella pyogenes ; Bubalus bubalis ; buffalo; BOX PCR; abortion, death DOLOCITEV GENOTIPA SEVA Trueperella pyogenes KOT GLAVNEGA POVZROCITELJA VNETJA MATERNICE, SPLAVOV IN SMRTI PRI VODNIH BIVOLIH ( Bubalus bubalis ) Povzetek: Trueperella pyogenes je vrsta komenzalnih bakterij, ki je prisotna v sluznicah zgornjih dihal, urogenitalnega trakta in sluznicah prebavil pri govedu. Ta vrsta lahko samostojno ali v povezavi z drugimi vnetnimi bakterijami povzroci vnetja in ogroža zdravje. Sistemska oblika bolezni s splavom in smrtjo pri vodnih bivolih se ni bila dokumentirana. Pri samici vodnega bivola ( Bubalus bubalis ) z vnetjem maternice in pljucnico smo izolirali sev T. pyogenes , ki je povzrocil splav in smrt prizadete živali. Raziskali smo virulencne gene in njihovo odpornost proti antibiotikom. Z metodo PCR smo potrdili prisotnost dobro znanih virulencnih genov T. pyogenes , vkljucno s plo , nan H, nan P, cbp A, fim A, fim C, fim E in fim G geni. Preizkus obcutljivosti na antibiotike je pokazal, da je bil ta izolat odporen proti tetraciklinu, eritromicinu in trimetoprim sulfametoksazolu. Poleg tega smo z metodo Box-PCR ugotovili, da se vzorec DNK tega izolata razlikuje od vzorca kontrolnega seva ( T. pyogenes ATCC 19411). Rezultati studije so pokazali, da lahko T. pyogenes povzroci sistemsko smrtonosno bolezen pri vodnih bivolih, vendar gostiteljski in okoljski pogoji pomebno vplivajo na razvoj okužbe. Kolikor vemo, je to prvo porocilo o bivolih s pljucnico, vnetjem maternice, splavom in smrtjo, ki jih je ","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":"57 1","pages":"5-14"},"PeriodicalIF":0.1,"publicationDate":"2020-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47319614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PARTICIPATION AND DRUG RESISTANCE OF COAGULASE-POSITIVE STAPHYLOCOCCI ISOLATED FROM CASES OF PYODERMA AND OTITIS EXTERNA IN DOGS","authors":"M. Szewczuk, S. Zych, P. Sablik","doi":"10.26873/svr-926-2019","DOIUrl":"https://doi.org/10.26873/svr-926-2019","url":null,"abstract":"Staphylococcus pseudintermedius is considered as a major pathogen in dogs, typically involved in skin and ear infections. Other staphylococci, as well as β-hemolytic streptococci, Pseudomonas aeruginosa or yeast-like fungi of the genus Malassezia also play an important role in inflammation. Because of this diversity, an appropriate choice of antimicrobial agent(-s) can be difficult. A total of 474 tests were performed (including 255 pyoderma and 219 otitis externa cases). In the case of pyoderma, 82.4% of skin lesions were caused by staphylococci. Co-infections with β-hemolytic streptococci (17.3 %), Malassezia sp . (15.7 %) and P . aeruginosa (4.3 %) were also recorded. For external ear infections, the share of staphylococci in inflammation was lower (44.8 %) than that of Malassezia sp . (58 %). Relatively frequent co-infections with β-hemolytic streptococci (18.8 %) and P. aeruginosa (7.8 %) were also noted. A total of 308 susceptibility tests for coagulase-positive staphylococci were performed (210 and 98 for skin and otitis externa , respectively). In ≥ 86 % of cases, amoxicillin potentiated with clavulanic acid, cephalexin and fluoroquinolones effectively inhibited the growth of all bacteria in vitro . A total of 25 isolates (24 S. pseudintermedius and one S. aureus ) were considered as methicillin-resistant. The mecA gene was identified in 100 % of those strains but only 44 % of the isolates additionally carried the bla Z gene. All mecA -positive staphylococci were multidrug-resistant, mainly to all β-lactams, fluoroquinolones, linkozamides, macrolides as well as sulfonamides but always susceptible to mupirocin. Overall, multidrug-resistance varied between 6 % ( otitis externa ) and 9 % (inflamed skin) and may become a significant problem in the future. Key words: pyoderma; otitis externa; dog; staphylococci; multidrug resistance; mecA , mupirocin PARTICIPATION AND DRUG RESISTANCE OF COAGULASE-POSITIVE STAPHYLOCOCCI ISOLATED FROM CASES OF PYODERMA AND OTITIS EXTERNA IN DOGS Povzetek: Staphylococcus pseudintermedius is considered as a major pathogen in dogs, typically involved in skin and ear infections. Other staphylococci, as well as β-hemolytic streptococci, Pseudomonas aeruginosa or yeast-like fungi of the genus Malassezia also play an important role in inflammation. Because of this diversity, an appropriate choice of antimicrobial agent(-s) can be difficult. A total of 474 tests were performed (including 255 pyoderma and 219 otitis externa cases). In the case of pyoderma, 82.4 % of skin lesions were caused by staphylococci. Co-infections with β-hemolytic streptococci (17.3 %), Malassezia sp . (15.7 %) and P . aeruginosa (4.3 %) were also recorded. For external ear infections, the share of staphylococci in inflammation was lower (44.8%) than that of Malassezia sp . (58 %). Relatively frequent co-infections with β-hemolytic streptococci (18.8 %) and P. aeruginosa (7.8 %) were also noted. A total of 308 susceptibility tests for coagulase-p","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":"90 14","pages":"33-43"},"PeriodicalIF":0.1,"publicationDate":"2020-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41243724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EGG WEIGHT AFFECTS HATCHING RESULTS, BODY WEIGHT AND FEAR-RELATED BEHAVIOR IN JAPANESE QUAILS","authors":"Magda I Abo-Samaha, S. E. El-kazaz","doi":"10.26873/svr-895-2019","DOIUrl":"https://doi.org/10.26873/svr-895-2019","url":null,"abstract":"This study was conducted to determine effect of egg weight on hatching results, chick performance and tonic immobility duration in Japanese Quails. Eggs were weighed individually and divided by weight into four groups: group 1 = 11.0 11.9 g, group 2 = 12.0 12.9 g, group 3 = 13.0 13.9 g and group 4 = 14.0 14.9 g. Egg weight loss during incubation and hatching parameters were recorded. Additionally, hatching weight and body weight were measured until 5 weeks and tonic immobility (TI) test was performed at the end of the experiment. Results revealed that egg weight loss during incubation decreased as egg weight increased. Group 2 was determined as the best suitable egg weight group in terms of fertility and group 2 and 3 in terms of hatchability of incubated eggs and hatchability of fertile eggs. Body weight increased as the egg weight increased and the heaviest chicks were hatched from heaviest egg group. Significant differences in TI duration between birds were noticed, as higher body weight birds had longer TI duration than birds with lower body weight. Thus, birds of high body weight were more fearful than birds of low body weight. It was concluded that the medium to heavy weight eggs are better eggs for hatching results and chick weight. Therefore, we recommend the selection of medium and heavy weight eggs for hatching implementations in","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":"57 1","pages":"15-24"},"PeriodicalIF":0.1,"publicationDate":"2020-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42718988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A MACROSCOPIC STUDY ON THE MUSCLES AND TENDONS OF FOREPAWS IN THE ANATOLIAN BOBCAT (Lynx lynx)","authors":"H. Ari, İ. Yurdakul, G. Aksoy","doi":"10.26873/svr-702-2019","DOIUrl":"https://doi.org/10.26873/svr-702-2019","url":null,"abstract":"This study was performed on the tendons and muscles of two Anatolian bobcats (Lynx lynx). The research materials were donated by Republic of Turkey Ministry of the Forestry and Water Affairs Sivas Branch Manager. To achieve this objective, dissection and radiography were applied to two dead specimens. Digit I is radiographically rudimentary in the forepaw. The muscles and tendons of the forepaw in the bobcat are located in the cranial and caudal aspect of the antebrachium, the central manus and hypothenar region. The extensor muscles are situated on the cranial aspect of the antebrachium, while the flexor muscles are located on the caudal aspect of the antebrachium. The m. extensor carpi radialis in the extensor group has long and short portions; the m. extensor digitorum lateralis et communis give rise to tendons associated with fingers; the fascia covering the muscles emerges on the lateral and medial border of the m. extensor digitorum lateralis. The m. flexor digitorum superficialis et profundus lie on the caudal aspect of the antebrachium; the superficial digital flexor tendons give rise to the origin of the bellies of the m. flexor digitorum brevis; the m. flexor digitorum profundus form a stout tendon from which the mm. lumbricales originate on the palmar aspect of carpal joints; the tendon of the muscle perforates the tendon of the m. flexor digitorum superficialis. The mm. flexores digitorum profundi breves in the central manus group consist of the lateral and medial portions and insert on the palmar aspect of each proximal phalanx associated with the fingers. Other muscles in the group, adductores digitorum, end to those in the fingers. Key words: Anatolian bobcat (Lynx lynx); forepaw; muscles; tendons; anatomy MAKROSKOPSKA STUDIJA KIT IN MISIC SPREDNJE SAPE PRI RISU ( Lynx lynx ) Povzetek: V raziskavi smo proucili kite in misice sprednje sape pri dveh risih ( Lynx lynx ). Material za raziskavo je darovalo Ministrstvo za gozdarstvo in vode Turcije. Na dveh kadavrih je bila izvedena radiografija in sekcija. Radiografija je pokazala, da je prvi prst sprednje sape rudimentaren. Misice in kite sprednje sape se pri risu nahajajo v kranialnem in kavdalnem podrocju podlahti, centralnega dela dlani in na podrocju misic, ki premikajo 5. prst. Misice iztegovalke se nahajajo na sprednjem delu podlahti, medtem ko so misice upogibalke namescene na kavdalnem podrocju podlahti. Ena izmed misic iztegovalk m. extensor carpi radialis ima dolge in kratke odseke; iz misice m. extensor digitorum lateralis et communis izhajajo kite za prste; fascija, ki pokriva misice pa se zacne na lateralnem in medialnem podrocju misice m. extensor digitorum lateralis . Misica m. flexor digitorum superficialis et profundus leži na kavdalnem podrocju podlahti; iz povrsinskih tetiv digitalnega fleksorja pa izhaja trebuh misice m. flexor digitorum brevis ; m. flexor digitorum profundus tvori mocno tetivo, iz katere mm. lumbricales izvirajo na palmarnem podrocju karpalnih skle","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":"56 1","pages":"153-162"},"PeriodicalIF":0.1,"publicationDate":"2019-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47052656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}