Captive chelonians should be fed a natural diet to achieve a growth rate similar to that of free-ranging animals. A wide range of commercially formulated foods dedicated to chelonians is available. Feeding commercial foods has the advantage of convenience. On the other hand, species-specific information on the nutritional requirements of chelonians is not available yet. The aim of this study was to analyse and evaluate commercial pellets and feeds for chelonians. Commercial pellets (ntortoise = 7, nturtle = 7, from 6 companies) dedicated to carnivorous aquatic turtles and herbivorous terrestrial tortoises, and other aquatic turtle feeds (lyophilised beef heart, dried aquatic invertebrates, and whole frozen fish) were bought in pet shops. Whole frozen fish served as a reference feed for carnivorous aquatic turtles. The chemical composition as well as calcium (Ca) and phosphorus (P) contents were determined. Single-sample t-test was used with the label information as null hypothesis and the results of own parallel analyses for crude protein (CP), ether extract (EE), crude fibre (CF), Ca and P. The labelling of some of the pellets was deficient as nutritive values, Ca or P data were missing (tortoise pellets: 4 out of 7; turtle pellets: 5 out of 7). The label data differed significantly (p<0.05) from the results of our own analysis for 13 out of the 14 pellets. None of the tortoise pellets met the requirements of the animals completely. Because of the inadequate Ca:P ratio only one turtle pellet could be accepted. Accordingly, none of the commercial pellets can be recommended as main or only feed. Key words: nutrition; pellet; metabolic bone disease; chelonian VREDNOTENJE KOMERCIALNIH ŽELV IN KRME ZA ŽELVE Izvleček: Želve v ujetništvu je potrebno hraniti z naravno krmo, da dosežejo podobno stopnjo rasti kot živali v prosti reji. Na voljo je širok izbor komercialno pripravljene hrane za želve. Prednost hranjenja želv s komercialno hrano je priročnost, vendar podatki o prehranskih potrebah za posamezne vrste želv še niso na voljo. Namen te raziskave je bil analizirati in ovrednotiti komercialne pelete in krmo za želve. V trgovinah za živali smo od 6 podjetij kupili komercialne pelete (npeleti za vodne želve = 7, npeleti za kopenkse želve = 7) za mesojede vodne in rastlinojede kopenske želve ter drugo krmo za vodne želve (liofilizirano goveje srce, posušene vodne nevretenčarje in zamrznjene cele ribe). Zamrznjene cele ribe smo uporabili kot referenčno krmo za mesojede vodne želve. Določili smo kemično sestavo in vsebnost kalcija (Ca) ter fosforja (P). Za ničelno hipotezo smo uporabili T-test enega vzorca s podatki na etiketi in rezultate lastne paralelne analize za surove beljakovine (an gl. crude proteins, CP), ekstrakt etra (angl. ether extract, EE), surovo vlaknino (angl. crude fibre, CF), Ca in P. Oznake nekaterih peletov so bile pomanjkljive, saj so manjkali podatki o hranilnih vrednostih, Ca in P (npeleti za kopenske želve = 4 od 7, npelet
圈养的龟类应该以自然饲料喂养,以达到与自由放养动物相似的生长速度。市面上有很多专门用于龟类的商业配方食品。喂养商业食品有方便的优点。另一方面,目前还没有关于龟类动物营养需求的具体资料。本研究的目的是分析和评价商品颗粒和饲料的龟。商业颗粒(ntortoise = 7, nturtle = 7,来自6家公司)专门用于肉食性水生龟和食草陆龟,以及其他水生龟饲料(冻干牛肉心,干水生无脊椎动物和整条冷冻鱼)在宠物商店购买。整条冷冻鱼作为食肉水生龟的参考饲料。测定了其化学组成及钙、磷含量。采用单样本t检验,以标签信息为原假设,并对粗蛋白质(CP)、粗脂肪(EE)、粗纤维(CF)、钙和磷进行了自己的平行分析。部分颗粒的营养价值标签不足,钙或磷数据缺失(乌龟颗粒:7个中有4个;标记数据与我们自己对14个颗粒中的13个的分析结果有显著差异(p<0.05)。没有一种龟丸完全符合动物的要求。由于钙磷比不足,只能接受一个龟丸。因此,不推荐任何一种商业颗粒作为主要或唯一饲料。关键词:营养学;颗粒;代谢性骨病;chelonian VREDNOTENJE KOMERCIALNIH ŽELV IN KRME ZA ŽELVEIzvleček: Želve v ujetništvu je potrebno hraniti z naravno krmo, da dosežejo podobno stopnjo rasti kot živali v prosti reji。Na voljo je širok izbor commercial - no pripravljene hranza želve。Prednost hranjenja želv s komercialno hranjenja prirost, vendar podatki o prehranskih potrebah za posamezne verste želv še niso na voljo。本文介绍了一种新的研究方法,用于分析在日本(želve)的过度消费和商业消费。V trgovinah za živali smo od 6 podjetij kupili komercialne pelete (npeleti za vodne želve = 7, npeleti za kopenkse želve = 7) za mesojede vodne in rastlinojede kopenske želve ter drugo krmo za vodne želve (liofilizirano goveje srce, posušene vodne nevreten arje in zamrznjene cele ribe)。Zamrznjene cele ribe smo uporabili kot referen no krmo za mesojede vodne želve。doloili smo kemi no sestavo in vsebnost kalcija (Ca) ter foforja (P). Za nielno hipotezo smo uporabili t检验;Za nielno hipotezo smo uporabili t检验;Za nielno hipotezo smo uporabili在rezonene平行分析;醚萃取物,EE);粗纤维(CF), P. Oznake nekaterih peletov so bile pomanjkljive, saj so manjkali podatki o hranilnih vrednostih, Ca in P (npeleti za kopenske želve = 4 od 7, npeleti za vodne želve = 5 od 7). podatki na etiketi so se bistveno razlikovali (P < 0.05) od rezultatov naše分析pri 13 od 14 vrst peletov。Nobeni peleti za kopenske želve niso v celoti izpolnjevali potreb živali。1 .浙江浙江大学学报(自然科学版):1 .浙江浙江大学学报(自然科学版):1 .浙江浙江大学学报(自然科学版):1 .浙江大学学报(自然科学版):1 .浙江大学学报(自然科学版);klju nebeede: prerana;peleti;Presnovna bolezen kosti;ž精灵
{"title":"EVALUATION OF COMMERCIAL TORTOISE AND TURTLE FEEDS","authors":"N. Hetényi, E. Andrásofszky","doi":"10.26873/svr-1216-2022","DOIUrl":"https://doi.org/10.26873/svr-1216-2022","url":null,"abstract":"Captive chelonians should be fed a natural diet to achieve a growth rate similar to that of free-ranging animals. A wide range of commercially formulated foods dedicated to chelonians is available. Feeding commercial foods has the advantage of convenience. On the other hand, species-specific information on the nutritional requirements of chelonians is not available yet. The aim of this study was to analyse and evaluate commercial pellets and feeds for chelonians. Commercial pellets (ntortoise = 7, nturtle = 7, from 6 companies) dedicated to carnivorous aquatic turtles and herbivorous terrestrial tortoises, and other aquatic turtle feeds (lyophilised beef heart, dried aquatic invertebrates, and whole frozen fish) were bought in pet shops. Whole frozen fish served as a reference feed for carnivorous aquatic turtles. The chemical composition as well as calcium (Ca) and phosphorus (P) contents were determined. Single-sample t-test was used with the label information as null hypothesis and the results of own parallel analyses for crude protein (CP), ether extract (EE), crude fibre (CF), Ca and P. The labelling of some of the pellets was deficient as nutritive values, Ca or P data were missing (tortoise pellets: 4 out of 7; turtle pellets: 5 out of 7). The label data differed significantly (p<0.05) from the results of our own analysis for 13 out of the 14 pellets. None of the tortoise pellets met the requirements of the animals completely. Because of the inadequate Ca:P ratio only one turtle pellet could be accepted. Accordingly, none of the commercial pellets can be recommended as main or only feed.\u0000Key words: nutrition; pellet; metabolic bone disease; chelonian\u0000 \u0000VREDNOTENJE KOMERCIALNIH ŽELV IN KRME ZA ŽELVE\u0000Izvleček: Želve v ujetništvu je potrebno hraniti z naravno krmo, da dosežejo podobno stopnjo rasti kot živali v prosti reji. Na voljo je širok izbor komercialno pripravljene hrane za želve. Prednost hranjenja želv s komercialno hrano je priročnost, vendar podatki o prehranskih potrebah za posamezne vrste želv še niso na voljo. Namen te raziskave je bil analizirati in ovrednotiti komercialne pelete in krmo za želve. V trgovinah za živali smo od 6 podjetij kupili komercialne pelete (npeleti za vodne želve = 7, npeleti za kopenkse želve = 7) za mesojede vodne in rastlinojede kopenske želve ter drugo krmo za vodne želve (liofilizirano goveje srce, posušene vodne nevretenčarje in zamrznjene cele ribe). Zamrznjene cele ribe smo uporabili kot referenčno krmo za mesojede vodne želve. Določili smo kemično sestavo in vsebnost kalcija (Ca) ter fosforja (P). Za ničelno hipotezo smo uporabili T-test enega vzorca s podatki na etiketi in rezultate lastne paralelne analize za surove beljakovine (an gl. crude proteins, CP), ekstrakt etra (angl. ether extract, EE), surovo vlaknino (angl. crude fibre, CF), Ca in P. Oznake nekaterih peletov so bile pomanjkljive, saj so manjkali podatki o hranilnih vrednostih, Ca in P (npeleti za kopenske želve = 4 od 7, npelet","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48140604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We evaluated the efficacy of supplementation with protein, yeast, or sugar candies of young honeybee colonies origi- nated from artificial swarms, by measuring strength and determining Nosema spp. spore level in adult bees in summer period. At the same time, we aimed to assess longevity of adult worker bees after feeding the same type of candies in controlled laboratory condition. The highest survival was found in Yeast and Protein candy group. On the contrary, the field study showed that artificial swarms produced significantly more pupae (2510.4 cm 2 , p=0.0001) in the 1 st period of measurement, and more larvae (964.8 cm 2 , p=0.003) and frames with bees (5.6, p=0.008) in the 2 nd period by feeding non-protein candy. In the 3rd period of evaluation of young colonies, the Sugar candy group had the highest number of frames covered by adult bees and honey stores, respectively (5.6, p=0.0009; 3432.0 cm 2, p=0.015). Sugar candy group produced the largest area of wax cells, however, the differences were not statistically significant. Nosema spp. spore level was checked quantitatively in adult bees. The lowest infection was statistically significant in Yeast candy group in June (4.35 million spores per bee, p=0.02), but insignificant in September. Supplementing artificial swarms Sugar candy offers the most promising potential for development of productive young colony. The findings of our study could help beekeepers to choose the effective candy supplement for optimal development of artificial swarms. Key words: Apis mellifera; artificial swarms; young colonies; supplements; candies; development; Nosema spp.; longevity RAZVOJ DRUŽIN IN ŠTEVILO SPOR Nosema spp. PRI MLADIH DRUŽINAH MEDONOSNE ČEBELE (Apis mellifera carnica, Pollmann 1879), KRMLJENIH S POGAČAMI Izvleček: Mlade čebelje družine iz umetnih rojev smo krmili z različnimi pogačami z dodatkom beljakovin, kvasa ali sladkorja. Ocenjevali smo razvoj družin in določali število spor Nosema spp. pri odraslih čebelah v poletnem obdobju. V laboratorijskih pogojih smo krmili čebele delavke z isto vrsto pogač. Najboljše preživetje smo ugotovili v skupinah, ki so prejele pogačo s kvasom oziroma beljakovinami. Nasprotno pa je poskus v družinah pokazal, da je bilo v 1. obdobju merjenja bistveno več bub (2510,4 cm 2 , p = 0,0001), v 2. obdobju pa več ličink (964,8 cm 2 , p = 0,003) in okvirjev s čebelami (5,6, p = 0,008) pri krmljenju s sladkorno pogačo. V 3. obdobju ocenjevanja mladih družin je imela skupina s sladkorno pogačo največ pokritih okvirjev z odraslimi čebelami in zalog medu (5,6, p = 0,0009; 3432,0 cm 2 , p = 0,015). Skupina s sladkorno pogačo je zgradila največjo površino satja, vendar razlike niso bile statistično značilne. Število spor Nosema spp. je bilo kvantitativno preverjeno pri odraslih čebelah. Najnižja okužba je bila statistično značilna v skupini s pogačo s kvasom v juniju (4,35 milijona spor na čebelo, p = 0,02), septembra pa spremembe niso bile signifikantne. Dodajanje sladkorne
我们通过测量夏季成年蜜蜂的强度和小孢子虫孢子水平,评估了在人工蜂群中形成的年轻蜜蜂菌落中补充蛋白质、酵母或糖的效果。同时,在实验室控制条件下,对工蜂饲喂相同类型糖果后的寿命进行了研究。酵母糖和蛋白糖组的存活率最高。与此相反,实地研究表明,人工蜂群在第1期的蛹产量(2510.4 cm 2, p=0.0001)显著高于人工蜂群;在第2期,饲喂非蛋白糖的人工蜂群的幼虫产量(964.8 cm 2, p=0.003)显著高于人工蜂群的幼虫产量(5.6,p=0.008)。在第三期的年轻群体评价中,糖糖组的成蜂和蜜库覆盖帧数最高,分别为5.6个,p=0.0009;3432.0 cm 2, p=0.015)。糖糖组产生的蜡细胞面积最大,但差异没有统计学意义。定量测定了小孢子虫在成蜂体内的孢子水平。酵母糖组在6月份的孢子感染率最低,为435万个/只,p=0.02,但在9月份的感染率最低,无统计学意义。补充人工蜂群的糖糖提供了最有潜力的生产年轻群体的发展。本研究结果可以帮助养蜂人选择有效的糖补充剂,以优化人工蜂群的发育。关键词:蜜蜂;人工成群;年轻的殖民地;补充;糖果;发展;疾病spp。长寿RAZVOJ DRUŽIN IN ŠTEVILO SPOR Nosema spp. PRI MLADIH DRUŽINAH MEDONOSNE ČEBELE (Apis mellifera carnica, Pollmann 1879), KRMLJENIH S POGAČAMI izvle ek: Mlade ebelje družine iz umetnih rojev smo krmili z razli nimi poga ami z dodatkom beljakovin, kvasa ali sladkorja。Ocenjevali smo razvoj družin in dolo ali število孢子Nosema sp . pri odraslih ebelah v poletnem obdobju。V laboratorijskih pogojih smo kmili, ebele delavke, z。Najboljše preživetje smo ugotovili v skupinah, ki so prejele poga o s kvasom oziroma beljakovinami。nasprono pa je poskus v družinah pokazal, da je billion v 1。Obdobju merjenja bistveno veje bub (2510,4 cm 2, p = 0,0001), v 2。Obdobju pa veje liink (964,8 cm 2, p = 0,003) in okvirjev s ebelami (5,6, p = 0,008) pri krmljenju s sladkorno poga o。V 3。Obdobju ocenjevanja mladih družin je imela skupina s sladkorno poga o najveei pokritih okvirjev z odraslimi ebelami in zalog medu (5,6, p = 0,0009);3432,0 cm 2, p = 0,015)。Skupina ' s sladkorno poga o je zgradila najve jo površino satja, vendar razlike niso数据统计 no zna ilne。Število spor Nosema sp . je bilo kantitivativno preverjeno pri odraslih ebelah。Najnižja okužba je bila statistical no zna v skupini s poga_ o s kvasom v juniju(4,35万jna spor na ebelo, p = 0,02), september pa spore_ (9);Dodajanje sladkorne pobeljih družin. Dodajanje sladkorne poga metmetm项目jem se je pokazalo kot najbolj obetavno za razvoj producktivnih mladih ebeljih družin。Ugotovitve naše študije bi lahko pomagale ebelarjem pri izbiri u inkovitega dodatka pogazizza optimalen razvoj metnih rojev。klju9 . nebeede:蜜蜂;umetni roji;mlade德鲁ž快乐;dodatki;pogače;razvoj;疾病spp。dolgoživost
{"title":"PERFORMANCE AND Nosema spp. SPORE LEVEL IN YOUNG HONEYBEE (Apis mellifera carnica, Pollmann 1879) COLONIES SUPPLEMENTED WITH CANDIES","authors":"M. I. Smodiš Škerl, I. Tlak Gajger","doi":"10.26873/svr-1498-2022","DOIUrl":"https://doi.org/10.26873/svr-1498-2022","url":null,"abstract":"We evaluated the efficacy of supplementation with protein, yeast, or sugar candies of young honeybee colonies origi- nated from artificial swarms, by measuring strength and determining Nosema spp. spore level in adult bees in summer period. At the same time, we aimed to assess longevity of adult worker bees after feeding the same type of candies in controlled laboratory condition. The highest survival was found in Yeast and Protein candy group. On the contrary, the field study showed that artificial swarms produced significantly more pupae (2510.4 cm 2 , p=0.0001) in the 1 st period of measurement, and more larvae (964.8 cm 2 , p=0.003) and frames with bees (5.6, p=0.008) in the 2 nd period by feeding non-protein candy. In the 3rd period of evaluation of young colonies, the Sugar candy group had the highest number of frames covered by adult bees and honey stores, respectively (5.6, p=0.0009; 3432.0 cm 2, p=0.015). Sugar candy group produced the largest area of wax cells, however, the differences were not statistically significant. Nosema spp. spore level was checked quantitatively in adult bees. The lowest infection was statistically significant in Yeast candy group in June (4.35 million spores per bee, p=0.02), but insignificant in September. Supplementing artificial swarms Sugar candy offers the most promising potential for development of productive young colony. The findings of our study could help beekeepers to choose the effective candy supplement for optimal development of artificial swarms.\u0000Key words: Apis mellifera; artificial swarms; young colonies; supplements; candies; development; Nosema spp.; longevity\u0000 \u0000RAZVOJ DRUŽIN IN ŠTEVILO SPOR Nosema spp. PRI MLADIH DRUŽINAH MEDONOSNE ČEBELE (Apis mellifera carnica, Pollmann 1879), KRMLJENIH S POGAČAMI \u0000Izvleček: Mlade čebelje družine iz umetnih rojev smo krmili z različnimi pogačami z dodatkom beljakovin, kvasa ali sladkorja. Ocenjevali smo razvoj družin in določali število spor Nosema spp. pri odraslih čebelah v poletnem obdobju. V laboratorijskih pogojih smo krmili čebele delavke z isto vrsto pogač. Najboljše preživetje smo ugotovili v skupinah, ki so prejele pogačo s kvasom oziroma beljakovinami. Nasprotno pa je poskus v družinah pokazal, da je bilo v 1. obdobju merjenja bistveno več bub (2510,4 cm 2 , p = 0,0001), v 2. obdobju pa več ličink (964,8 cm 2 , p = 0,003) in okvirjev s čebelami (5,6, p = 0,008) pri krmljenju s sladkorno pogačo. V 3. obdobju ocenjevanja mladih družin je imela skupina s sladkorno pogačo največ pokritih okvirjev z odraslimi čebelami in zalog medu (5,6, p = 0,0009; 3432,0 cm 2 , p = 0,015). Skupina s sladkorno pogačo je zgradila največjo površino satja, vendar razlike niso bile statistično značilne. Število spor Nosema spp. je bilo kvantitativno preverjeno pri odraslih čebelah. Najnižja okužba je bila statistično značilna v skupini s pogačo s kvasom v juniju (4,35 milijona spor na čebelo, p = 0,02), septembra pa spremembe niso bile signifikantne. Dodajanje sladkorne","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43311180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Majlind Sulce, A. Munga, Doriana Beqiraj, E. Ozuni, P. Zalla, G. Muça, X. Koleci
Lymphomas, leukemias and mast cell tumors belong to the most important group among all neoplasms affecting dog species. Diagnosis, staging and determining the cell type involved in a specific tumor represent a challenge for researchers and clinicians, and plays a crucial role in treatment efficacy and prognostic purposes. Many different gold standard techniques such as cytology, histopathology, immunohistochemistry and cytochemistry are used to routinely diagnose and stage these tumors. In the recent years flow cytometry is becoming more applicable in veterinary medicine since a wide number of health conditions can be analyzed in a short period of time with a high accuracy. Multiparametric analysis performed by flow cytometry is considered as one of the main advantages of this technique since cell populations can be analyzed for different superficial markers at the same time. Immunophenotyping and staging of tumor cell populations performed by flow cytometry can help in reaching a confirmatory diagnosis and appropriate prognosis of the disease. Moreover, many flow cytometric results have been linked to a high prognostic relevance especially in neoplastic disorders. However, flow cytometry results are compatible and should be interpreted in compliance with data obtained by histopathology, immunohistochemistry and cytology. Key words: flow cytometry; antibodies; diagnosis; lymphoma; leukemia UPORABNOST PRETOČNE CITOMETRIJE PRI PREPOZNAVANJU IN DOLOČANJU STADIJA LIMFOMA, LEVKEMIJE IN TUMORJEV MASTOCITOV PRI PSIH – PREGLED Izvleček: Limfomi, levkemije in tumorji mastocitov so najpomembnejše skupine neoplazem, ki prizadenejo pse. Diagnostika, določanje stopenj tumorja in tipa celic v določenem tumorju predstavljajo izziv za raziskovalce in klinike in igrajo ključno vlogo pri učinkovitosti zdravljenja in postavljanju prognoze. Za rutinsko diagnosticiranje in določanje stopenj teh tumorjev se uporablja veliko različnih temeljnih metod, kot so citologija, histopatologija, imunohistokemija in citokemija. V zadnjih letih je pretočna citometrija vse bolj uporabljana metoda v veterinarski medicini, saj je mogoče v kratkem času in z visoko natančnostjo analizirati veliko število zdravstvenih stanj. Ena izmed najpomembnejših prednosti te tehnike je multiparametrična analiza, s katero lahko v populaciji celic istočasno analiziramo različne površinske označevalce. Določanje površinskih označevalcev in stopenj populacij tumorskih celic s pretočno citometrijo lahko pripomore k potrditvi diagnoze in postavitvi ustrezne prognoze bolezni. Številni rezultati pretočne citometrije so imeli pomemben prognostični pomen zlasti pri neoplastičnih obolenjih. Vendar je rezultate pretočne citometrije potrebno združiti in razlagati v skladu s podatki, pridobljenimi s histopatologijo, imunohistokemijo in citologijo. Ključne besede: pretočna citometrija; protitelesa; diagnoza; limfom; levkemija
淋巴瘤、白血病和肥大细胞瘤是影响犬种的所有肿瘤中最重要的一类。诊断、分期和确定特定肿瘤的细胞类型对研究人员和临床医生来说是一个挑战,在治疗效果和预后目的中起着至关重要的作用。许多不同的金标准技术,如细胞学、组织病理学、免疫组织化学和细胞化学,被用于常规诊断和分期这些肿瘤。近年来,流式细胞术越来越多地应用于兽医学,因为它可以在短时间内以高精度分析大量的健康状况。流式细胞术进行的多参数分析被认为是该技术的主要优点之一,因为细胞群可以同时分析不同的表面标记物。流式细胞术对肿瘤细胞群进行免疫分型和分期可以帮助达到疾病的确诊和适当的预后。此外,许多流式细胞术结果与高度预后相关,特别是在肿瘤疾病中。然而,流式细胞术结果是兼容的,应该与组织病理学、免疫组织化学和细胞学获得的数据相一致。关键词:流式细胞术;抗体;诊断;淋巴瘤;白血病UPORABNOST PRETOČNE CITOMETRIJE PRI PREPOZNAVANJU IN DOLOČANJU STADIJA LIMFOMA, LEVKEMIJE IN TUMORJEV MASTOCITOV PRI PSIH - pregledizvleek: Limfomi, LEVKEMIJE IN tumor masocitov so najpomembnejše skupine neoplazem, ki prizadenejo pse。诊断上,在tipa celic中,doloanje stopenj肿瘤;doloenem肿瘤在igrajo klju中,在klinike中,没有vlogo和uinkovitosti zdravljenja在avljanju后预后中。研究结果表明:1 .诊断方法:1 .诊断方法:1 .诊断方法:1 .诊断方法:1 .诊断方法:1 .诊断方法:1 .诊断方法:1 .诊断方法:1 .诊断方法:V zadnjih letih je prepreto na citometrija vse bolj uporabjana metoda V veterinarmedicini, saj j mogoj V kratkem asu in z visoko natanannostojo analizirati veliko število zdravstvenini stanj。[1] [1] [1] [1] [1] [1] [1] [1] [1] [1] [1] [1] [1] [1] [1] [1] [1] [1] [3] [1] [1] [3] [1] [3] [1] [1] [4]dolo anje površinskih ozna在急性流行性肿瘤中的应用价值(p < 0.05)。Številni rezultati preto ne citometrije so imeli pomemben prognosti_ () ni pomenzlasti pri neoplasti_ () nih obolenjih。Vendar je调控 ne citometrije potrebno združiti在razlagati, skladu ' s podatki, pridobljenimi ' s组织学,免疫组织学中的citometrije potrebno。klju nebeede: pretona citometrija;protitelesa;diagnoza;limfom;levkemija
{"title":"APPLICABILITY OF FLOW CYTOMETRY IN IDENTIFYING AND STAGING LYMPHOMA, LEUKEMIA AND MAST CELL TUMORS IN DOGS: AN OVERVIEW","authors":"Majlind Sulce, A. Munga, Doriana Beqiraj, E. Ozuni, P. Zalla, G. Muça, X. Koleci","doi":"10.26873/svr-1372-2022","DOIUrl":"https://doi.org/10.26873/svr-1372-2022","url":null,"abstract":"Lymphomas, leukemias and mast cell tumors belong to the most important group among all neoplasms affecting dog species. Diagnosis, staging and determining the cell type involved in a specific tumor represent a challenge for researchers and clinicians, and plays a crucial role in treatment efficacy and prognostic purposes. Many different gold standard techniques such as cytology, histopathology, immunohistochemistry and cytochemistry are used to routinely diagnose and stage these tumors. In the recent years flow cytometry is becoming more applicable in veterinary medicine since a wide number of health conditions can be analyzed in a short period of time with a high accuracy. Multiparametric analysis performed by flow cytometry is considered as one of the main advantages of this technique since cell populations can be analyzed for different superficial markers at the same time. Immunophenotyping and staging of tumor cell populations performed by flow cytometry can help in reaching a confirmatory diagnosis and appropriate prognosis of the disease. Moreover, many flow cytometric results have been linked to a high prognostic relevance especially in neoplastic disorders. However, flow cytometry results are compatible and should be interpreted in compliance with data obtained by histopathology, immunohistochemistry and cytology.\u0000Key words: flow cytometry; antibodies; diagnosis; lymphoma; leukemia\u0000 \u0000UPORABNOST PRETOČNE CITOMETRIJE PRI PREPOZNAVANJU IN DOLOČANJU STADIJA LIMFOMA, LEVKEMIJE IN TUMORJEV MASTOCITOV PRI PSIH – PREGLED\u0000Izvleček: Limfomi, levkemije in tumorji mastocitov so najpomembnejše skupine neoplazem, ki prizadenejo pse. Diagnostika, določanje stopenj tumorja in tipa celic v določenem tumorju predstavljajo izziv za raziskovalce in klinike in igrajo ključno vlogo pri učinkovitosti zdravljenja in postavljanju prognoze. Za rutinsko diagnosticiranje in določanje stopenj teh tumorjev se uporablja veliko različnih temeljnih metod, kot so citologija, histopatologija, imunohistokemija in citokemija. V zadnjih letih je pretočna citometrija vse bolj uporabljana metoda v veterinarski medicini, saj je mogoče v kratkem času in z visoko natančnostjo analizirati veliko število zdravstvenih stanj. Ena izmed najpomembnejših prednosti te tehnike je multiparametrična analiza, s katero lahko v populaciji celic istočasno analiziramo različne površinske označevalce. Določanje površinskih označevalcev in stopenj populacij tumorskih celic s pretočno citometrijo lahko pripomore k potrditvi diagnoze in postavitvi ustrezne prognoze bolezni. Številni rezultati pretočne citometrije so imeli pomemben prognostični pomen zlasti pri neoplastičnih obolenjih. Vendar je rezultate pretočne citometrije potrebno združiti in razlagati v skladu s podatki, pridobljenimi s histopatologijo, imunohistokemijo in citologijo.\u0000Ključne besede: pretočna citometrija; protitelesa; diagnoza; limfom; levkemija","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":"1 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41967141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of the present study was to obtain information about the experiences of Slovenian pet owners on the use of cannabidiol (CBD) products in their pets. An open online survey targeted Slovenian owners of cats and dogs who have used CBD to treat their pets. Questions pertained to demographic data, animal data, health status of the animals, CBD formulations and experiences with use. Descriptive statistics and frequency distributions were performed using the survey software. A total of 41 respondents participated in the survey, most of whom were female (87.8 %) and between 31 and 50 years old (56.1 %). Most respondents (90.2 %) were dog owners. Cannabidiol (CBD)-based products were mainly used to treat orthopedic and oncologic conditions, as adjunctive therapy to other medications. Oil formulations were used by most dog (85.2 %) and all cat owners. Participants predominantly re-ported positive effects, such as improved well-being, increased activity, and reduced pain. The results suggest that Slovenian pet owners who used CBD-based products as a treatment for their pets were overall satisfied with the effects of these products. However, there were still reports of some adverse effects, such as drowsiness, increased appetite, and thirst. Further research is essential to improve practices related to cannabis-based medicines for pets, especially CBD, and to put an end to the trial- and error- based therapeutic approach of pet owners and veterinarians. Long-term, large-scale research studies are needed to clarify the role of CBD as a treatment option for osteoarthritis, chronic pain, cancer, behavioral problems, and other chronic inflammatory conditions in dogs and cats. Key words: cannabis; cannabidiol; cats; dogs; survey UPORABA KANABIDIOLA PRI LASTNIKIH HIŠNIH LJUBLJENČKOV V SLOVENIJI – ANKETNA ŠTUDIJA Izvleček: Namen raziskave je bil pridobiti informacije o izkušnjah slovenskih lastnikov psov in mačk z uporabo izdelkov s kanabidiolom (CBD) pri svojih živalih. Raziskavo smo izvedli na podlagi anonimne spletne ankete, ki je bila namenjena slovenskim lastnikom psov in mačk, ki so uporabljali CBD za zdravljenje svojih živali. Vprašanja so se nanašala na demografske podatke, podatke o živalih, njihovem zdravstvenem stanju, vrste pripravkov s CBD-jem in izkušnjah z uporabo. Za opisno statistiko in frekvenčno porazdelitev smo uporabili programsko opremo za anketiranje. V raziskavi je sodelovalo 41 anketirancev, med katerimi je bilo največ žensk (87,8%) in starih med 31 in 50 let (56,1%). Večina anketirancev (90,2%) je bila lastnikov psov. Izdelki na osnovi CBD-ja so se večinoma uporabljali za zdravljenje ortopedskih in onkoloških obolenj kot dopolnilno zdravljenje. Največ lastnikov psov (85,2%) in vsi lastniki mačk so uporabljali oljne pripravke. Sodelujoči so večinoma poročali o pozitivnih učinkih, kot so boljše počutje, povečanje aktivnosti in zmanjšanje bolečine. Rezultati kažejo, da so slovenski lastniki psov in mačk, ki uporabljajo izdelke na o
本研究的目的是获得斯洛文尼亚宠物主人对其宠物使用大麻二酚(CBD)产品的经验信息。一项公开的在线调查针对斯洛文尼亚猫狗的主人,他们用CBD治疗他们的宠物。问题涉及人口统计数据、动物数据、动物健康状况、CBD配方和使用经验。使用调查软件进行描述性统计和频率分布。共有41名受访者参与了调查,其中大多数是女性(87.8%),年龄在31至50岁之间(56.1%)。大多数受访者(90.2%)是狗主人。以大麻二酚(CBD)为基础的产品主要用于治疗骨科和肿瘤疾病,作为其他药物的辅助治疗。大多数狗(85.2%)和所有猫的主人都使用油制剂。参与者主要报告了积极的影响,比如改善了幸福感,增加了活动量,减轻了疼痛。结果表明,斯洛文尼亚的宠物主人使用基于大麻二酚的产品作为治疗宠物的方法,总体上对这些产品的效果感到满意。然而,仍有一些不良反应的报道,如嗜睡、食欲增加和口渴。进一步的研究对于改善以大麻为基础的宠物药物的实践,特别是CBD,以及结束宠物主人和兽医基于试验和错误的治疗方法至关重要。需要长期、大规模的研究来阐明CBD作为治疗狗和猫的骨关节炎、慢性疼痛、癌症、行为问题和其他慢性炎症的选择的作用。关键词:大麻;大麻二酚;猫;狗;调查UPORABA KANABIDIOLA PRI LASTNIKIH HIŠNIH LJUBLJENČKOV V SLOVENIJI - ANKETNA ŠTUDIJA izvleek: Namen raziskave je bil pridobiti informacije o izkušnjah slovenskih lastnikov psov in mak z uporabo izdelkov s kanabidiolom (CBD) PRI svojih živalih。Raziskavo smo izvedli na podlagi anonimne spleitne ankete, ki je bila namenjena slovenskim lastnikom psov in makk, ki so uporabljali CBD za zdravljenje svojih živali。Vprašanja so se nanašala na demografske podatke, podatke o živalih, njihovem zdravstvenem stanju, vrste pripravkov的CBD-jem in izkušnjah z uporabo。在frekven.com上的一项民意调查显示,没有任何一项民意调查显示,这项调查的结果是不可靠的。V raziskavi je sodelovalo 41 anketirancev, med katerimi je bilo najveev žensk (87,8%) in starih med 31 in 50 let(55,1%)。ve ina anketirancev(90.2%)由拉斯特尼科夫psov领导。Izdelki na osnovi CBD-ja so se veinoma uporababjali za zdravljenje或topedskih在onkoloških obolenj kot dopolnilno zdravljenje。najveniki lastnikov psov(85,2%)在vsi lastniki ma k所以uporporabli oljne pripravke。sodeleljo i so vezitivniu inkih, kot so boljše pozitivniuutje, pove anjeaktivnosti in zmanjšanje bole。Rezultati kažejo, da so slovenski lastniki psov in mak, ki uporababjjo izdelke na osnovi CBD-ja za zdravljenje svojih živali, na splošno zadovoljni z uinki the proizvodov。poroali so tudi o nekaterih neželenih u inkih, kot so zaspanost, pove和食欲žeja。Nadaljnje raziskave so bistvenega omena izboljšanje praks uporabe zdravil na osnovi konoplje za pse in ma, zlasti CBD, in za odpravo terapevtskega pristopa lastnikov in veterinary jev, ki temelji na poskusih in napakah。dolgoro ne in obširne raziskave so potrebne za jasno prepredelitev vloge CBD-ja pri zdravljenju kroninih bole in, raka, vedenjskih težav,骨关节炎in drugs kroni nih vnetnih stanj pri psih in makah。klju: konoplja;kanabidiol;马č客;常设调查小组委员会;anketa
{"title":"USE OF CANNABIDIOL PRODUCTS BY PET OWNERS IN SLOVENIA: A SURVEY-BASED STUDY","authors":"Nina Milevoj, N. Tozon, Katerina Tomsič","doi":"10.26873/svr-1294-2022","DOIUrl":"https://doi.org/10.26873/svr-1294-2022","url":null,"abstract":"The aim of the present study was to obtain information about the experiences of Slovenian pet owners on the use of cannabidiol (CBD) products in their pets. An open online survey targeted Slovenian owners of cats and dogs who have used CBD to treat their pets. Questions pertained to demographic data, animal data, health status of the animals, CBD formulations and experiences with use. Descriptive statistics and frequency distributions were performed using the survey software. A total of 41 respondents participated in the survey, most of whom were female (87.8 %) and between 31 and 50 years old (56.1 %). Most respondents (90.2 %) were dog owners. Cannabidiol (CBD)-based products were mainly used to treat orthopedic and oncologic conditions, as adjunctive therapy to other medications. Oil formulations were used by most dog (85.2 %) and all cat owners. Participants predominantly re-ported positive effects, such as improved well-being, increased activity, and reduced pain. The results suggest that Slovenian pet owners who used CBD-based products as a treatment for their pets were overall satisfied with the effects of these products. However, there were still reports of some adverse effects, such as drowsiness, increased appetite, and thirst. Further research is essential to improve practices related to cannabis-based medicines for pets, especially CBD, and to put an end to the trial- and error- based therapeutic approach of pet owners and veterinarians. Long-term, large-scale research studies are needed to clarify the role of CBD as a treatment option for osteoarthritis, chronic pain, cancer, behavioral problems, and other chronic inflammatory conditions in dogs and cats.\u0000Key words: cannabis; cannabidiol; cats; dogs; survey\u0000 \u0000UPORABA KANABIDIOLA PRI LASTNIKIH HIŠNIH LJUBLJENČKOV V SLOVENIJI – ANKETNA ŠTUDIJA \u0000Izvleček: Namen raziskave je bil pridobiti informacije o izkušnjah slovenskih lastnikov psov in mačk z uporabo izdelkov s kanabidiolom (CBD) pri svojih živalih. Raziskavo smo izvedli na podlagi anonimne spletne ankete, ki je bila namenjena slovenskim lastnikom psov in mačk, ki so uporabljali CBD za zdravljenje svojih živali. Vprašanja so se nanašala na demografske podatke, podatke o živalih, njihovem zdravstvenem stanju, vrste pripravkov s CBD-jem in izkušnjah z uporabo. Za opisno statistiko in frekvenčno porazdelitev smo uporabili programsko opremo za anketiranje. V raziskavi je sodelovalo 41 anketirancev, med katerimi je bilo največ žensk (87,8%) in starih med 31 in 50 let (56,1%). Večina anketirancev (90,2%) je bila lastnikov psov. Izdelki na osnovi CBD-ja so se večinoma uporabljali za zdravljenje ortopedskih in onkoloških obolenj kot dopolnilno zdravljenje. Največ lastnikov psov (85,2%) in vsi lastniki mačk so uporabljali oljne pripravke. Sodelujoči so večinoma poročali o pozitivnih učinkih, kot so boljše počutje, povečanje aktivnosti in zmanjšanje bolečine. Rezultati kažejo, da so slovenski lastniki psov in mačk, ki uporabljajo izdelke na o","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46742026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Karabağ, S. Alkan, T. Karslı, C. İkten, I. Sahin, M. Mendeș
Abstract: This study was carried out to investigate the effects of selection on the single nucleotide polymorphisms (SNPs) in coding sequence of leptin receptor (LEPR) gene and possible associations between SNPs’ and some meat yield traits of Japanese quail lines. Fifteen generations divergently selected two lines (HBW and LBW) for 5-weeks of age body weight and a control were used as materials for this study. A 348-bp part of the LEPR coding region (18th exon) were sequenced in a total of 113 individuals from the three quail lines and shown that the fragments contained four SNPs loci (T490C, C528T, G537A, T571C) and five haplotypes (TTGT, CTGT, TCGT, TCAT, TCAC). T490C replacement caused the missense mutation of phenylalanine to convert to leucine (PheLeu). However, other SNPs were synonymous and there were no changes in transcripts. It was determined that the quails with higher phenotypic values were in the TT genotype at the T390C locus. Statistical analyses showed that there were significant differences among the quail lines, SNP alleles and haplotypes in terms of interested phenotypic traits (P0.05), and also SNP and haplotype distributions changed depending on quail lines (P0.001). When all results were evaluated together, it was concluded that the fifteen generations of selection caused significant changes in the LEPR gene in terms of economically important traits in Japanese quail lines (C. coturnix japonica). Key words: SNP; haplotype; leptin receptor; selection; Japanese quail0.05) and also SNP and haplotype distributions changed depending on quail lines (P0.001). When all results were evaluated together, it was concluded that the fifteen generations of selection caused significant changes in the LEPR gene in terms of economically important traits in Japanese quail lines (C. coturnix japonica).Key words: SNP; haplotype; leptin receptor; selection; Japanese quailUČINKI SELEKCIJE LINIJ JAPONSKIH PREPELIC NA GEN ZA LEPTINSKI RECEPTOR, POVEZAN S PRIREJO MESAIzvleček: Ta študija je bila izvedena z namenom raziskati učinke selekcije na polimorfizme posameznih nukleotidov (Angl., Single Nucleotide Polymorphism, SNP) v kodnem zaporedju gena za leptinski receptor (LEPR) in možne povezave med SNP in nekaterimi značilnostmi prireje mesa pri japonskih prepelicah. V študiji je bilo poleg kontrole uporabljenih petnajst generacij različno izbranih dveh linij (HBW in LBW) s telesno maso pri starosti petih tednov. 348-bp del kodne regije LEPR (18. ekson) je bil sekvenciran pri skupno 113 posameznikih iz treh linij prepelic, fragmenti pa so vsebovali štiri lokuse SNP (T490C, C528T, G537A, T571C) in pet haplotipov (TTGT, CTGT, TCGT, TCAT, TCAC). Zamenjava T490C je povzročila drugačnosmiselno mutacijo fenilalanina v levcin (Phe Leu), vendar so bili drugi SNP-ji sinonimni in v transkriptih ni bilo sprememb. Ugotovljeno je bilo, da so prepelice z višjimi fenotipskimi vrednostmi imele genotip TT na lokusu T390C. Statistične analize so z vidika fenotipskih las
摘要本试验旨在研究选择对日本鹌鹑瘦素受体(LEPR)基因编码序列单核苷酸多态性(snp)的影响,以及该多态性与日本鹌鹑某些肉品产量性状的可能关联。以5周龄体重和对照为材料,采用15代差异性选择HBW和LBW 2个系。对3个鹌鹑系113个个体的LEPR编码区(第18外显子)348 bp片段进行了测序,结果显示该片段包含4个snp位点(T490C、C528T、G537A、T571C)和5个单倍型(TTGT、CTGT、TCGT、TCAT、TCAC)。T490C替换导致苯丙氨酸错义突变转化为亮氨酸(PheLeu)。然而,其他snp是同义的,转录本没有变化。结果表明,表型值较高的鹌鹑在T390C位点属于TT基因型。统计分析表明,不同鹌鹑系、SNP等位基因和单倍型在感兴趣表型性状上存在显著差异(P0.05), SNP和单倍型分布在不同的鹌鹑系之间存在差异(P0.001)。综合评价结果表明,15代的选择使日本鹌鹑LEPR基因在经济性状上发生了显著变化。关键词:SNP;单体型;瘦素受体;选择;日本鹌鹑(0.05),SNP和单倍型分布也随鹌鹑品系的不同而变化(P0.001)。综合评价结果表明,15代的选择使日本鹌鹑LEPR基因在经济性状上发生了显著变化。关键词:SNP;单体型;瘦素受体;选择;日语quailUČINKI SELEKCIJE LINIJ JAPONSKIH preelic NA GEN ZA LEPTINSKI受体,POVEZAN S PRIREJO mesaizvleek: a študija je bila izvedena z namenom raziskati uinke SELEKCIJE NA polimorfizme posameznih nukleotidov [j]。(1)单核苷酸多态性(SNP)与nekaterimi zna ilnosmi prireje mesa pri japonskih preelicah的单核苷酸多态性(SNP)Vštudiji我bilo poleg kontrole uporabljenih petnajst generacij različ没有izbranih dveh linij(激光焊HBW)年代telesno石匠给pri starosti petih tednov。348 bp del kodne regije LEPR(18)。ekson)我bil sekvenciran pri skupno 113 posameznikih工业区treh linij prepelic, fragmenti pa所以vsebovalištiri lokuse SNP (T490C、C528T G537A, T571C)宠物haplotipov (TTGT, CTGT、TCGT TCAT TCAC)。Zamenjava T490C je povzro ila druga nosmmiselno mutacijo fenilalanina v levcin (Phe Leu), vendar so bili drug SNP-ji sinonimni在v转录序列中的表达。Ugotovljeno je十亿,数据所以preplice z višjimi fenotipskimi vrednosmiele gentip TT na lokusu T390C。统计学上,我们分析了so z vidika fenotipskih lastnosti pokazale zna ilne razlike med linijami preelic, aleli SNP in haplotipi (P 0.05), med linijami preelic pa je bila razli na tudi porazdelitev SNP in haplotipov (P 0.001)。Na podlagi vrednotenja vseh rezultatov smo ugotovili, da je selekcija petnajstih generacij linij japonskih preelic (C. coturnix japonica) povzro klju ne sprememberv genu LEPR, povezanim z gospodarsko pomembnimi lastnotmi preelic。klju nebeede: SNP;haplotip;leptinski受体;selekcija;japosnka prepelica
{"title":"EFFECTS OF SELECTION IN TERMS OF MEAT YIELD TRAITS ON LEPTIN RECEPTOR GENE IN JAPANESE QUAIL LINES","authors":"K. Karabağ, S. Alkan, T. Karslı, C. İkten, I. Sahin, M. Mendeș","doi":"10.26873/svr-1316-2022","DOIUrl":"https://doi.org/10.26873/svr-1316-2022","url":null,"abstract":"Abstract: This study was carried out to investigate the effects of selection on the single nucleotide polymorphisms (SNPs) in coding sequence of leptin receptor (LEPR) gene and possible associations between SNPs’ and some meat yield traits of Japanese quail lines. Fifteen generations divergently selected two lines (HBW and LBW) for 5-weeks of age body weight and a control were used as materials for this study. A 348-bp part of the LEPR coding region (18th exon) were sequenced in a total of 113 individuals from the three quail lines and shown that the fragments contained four SNPs loci (T490C, C528T, G537A, T571C) and five haplotypes (TTGT, CTGT, TCGT, TCAT, TCAC). T490C replacement caused the missense mutation of phenylalanine to convert to leucine (PheLeu). However, other SNPs were synonymous and there were no changes in transcripts. It was determined that the quails with higher phenotypic values were in the TT genotype at the T390C locus. Statistical analyses showed that there were significant differences among the quail lines, SNP alleles and haplotypes in terms of interested phenotypic traits (P0.05), and also SNP and haplotype distributions changed depending on quail lines (P0.001). When all results were evaluated together, it was concluded that the fifteen generations of selection caused significant changes in the LEPR gene in terms of economically important traits in Japanese quail lines (C. coturnix japonica). Key words: SNP; haplotype; leptin receptor; selection; Japanese quail0.05) and also SNP and haplotype distributions changed depending on quail lines (P0.001). When all results were evaluated together, it was concluded that the fifteen generations of selection caused significant changes in the LEPR gene in terms of economically important traits in Japanese quail lines (C. coturnix japonica).Key words: SNP; haplotype; leptin receptor; selection; Japanese quailUČINKI SELEKCIJE LINIJ JAPONSKIH PREPELIC NA GEN ZA LEPTINSKI RECEPTOR, POVEZAN S PRIREJO MESAIzvleček: Ta študija je bila izvedena z namenom raziskati učinke selekcije na polimorfizme posameznih nukleotidov (Angl., Single Nucleotide Polymorphism, SNP) v kodnem zaporedju gena za leptinski receptor (LEPR) in možne povezave med SNP in nekaterimi značilnostmi prireje mesa pri japonskih prepelicah. V študiji je bilo poleg kontrole uporabljenih petnajst generacij različno izbranih dveh linij (HBW in LBW) s telesno maso pri starosti petih tednov. 348-bp del kodne regije LEPR (18. ekson) je bil sekvenciran pri skupno 113 posameznikih iz treh linij prepelic, fragmenti pa so vsebovali štiri lokuse SNP (T490C, C528T, G537A, T571C) in pet haplotipov (TTGT, CTGT, TCGT, TCAT, TCAC). Zamenjava T490C je povzročila drugačnosmiselno mutacijo fenilalanina v levcin (Phe Leu), vendar so bili drugi SNP-ji sinonimni in v transkriptih ni bilo sprememb. Ugotovljeno je bilo, da so prepelice z višjimi fenotipskimi vrednostmi imele genotip TT na lokusu T390C. Statistične analize so z vidika fenotipskih las","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42522369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract: Peste des petits ruminants (PPR) is World Organization for Animal Health (OIE) notifiable, economically important transboundary, highly contagious, and an acute viral disease of small ruminants. The disease is caused by the PPR virus (PPRV). PPRV belongs to the genus Morbillivirus of the family Paramyxoviridae. The recent epidemiological and molecular characterization of PPR virus isolates subdivides them into four genetically distinct lineages (I, II, III and IV). The disease is endemic across Asia, the Middle East and African regions and is considered a major obstacle to the development of sustainable agriculture across the developing world due to its huge burden on the economy and development of the affected countries and has recently been targeted by the Food and Agriculture Organization (FAO) and the OIE for global eradication by 2030. PPR-endemic countries should join the regional force, and implement regional road maps for the progressive and successful control and elimination of PPRV. In this review, the regional epidemiology of PPR outbreaks and associated risk factors, including animal factors (age, species and sex), environmental factors (season, spatial distribution of disease in various locations) and trade associated factors with special reference to the PPR affected countries in South, Central and East Asia are comprehensively discussed.Key words: epidemiology; PPR-virus; risk factors; control; eradication; AsiaREGIONALNA EPIDEMIOLOGIJA IN Z NJO POVEZANI DEJAVNIKI TVEGANJA ZA KUGO DROBNICE V AZIJI – PREGLEDIzvleček: Kuga drobnice (angl., Peste des petits ruminants, PPR) je gospodarsko pomembna, čezmejno nevarna, visoko nalezljiva, akutna virusna bolezen drobnice, ki jo je potrebno prijaviti Svetovni organizaciji za zdravje živali (OIE). Bolezen povzroča virus PPR (PPRV). PPRV spada v rod virusov Morbillivirus iz družine Paramyxoviridae. Nedavna epidemiološka in molekularna karakterizacija izolatov virusa PPRV deli izolate na štiri genetsko različne linije (I, II, III in IV). Bolezen je endemična v Aziji, na bližnjem vzhodu in v Afriki. Zaradi močnega bremena za gospodarstvo in razvoj prizadetih držav velja za glavno oviro pri razvoju trajnostnega kmetijstva v državah v razvoju. Organizacija Združenih narodov za prehrano in kmetijstvo (FAO) in OIE sta nedavno določili, da je bolezen potrebno do leta 2030 izkoreniniti po vsem svetu. Države z endemijo PPR bi se morale pridružiti regionalnim silam in izvajati regionalne načrte za postopen in uspešen nadzor in izkoreninjenje PPRV. V tem pregledu so izčrpno obravnavani regionalna epidemiologija izbruhov PPR in povezani dejavniki tveganja, vključno z dejavniki živali (starost, vrsta in spol), okolja (letni čas, prostorska razširjenost bolezni na različnih lokacijah) in trgovine, s posebnim poudarkom na državah s PPR v južni, osrednji in vzhodni Aziji.Ključne besede: epidemiologija; virus PPR; dejavniki tveganja; nadzor; izkoreninjenje; Azija
{"title":"REGIONAL EPIDEMIOLOGY AND ASSOCIATED RISK FATORS OF PESTE DES PETITS RUMINANTS IN ASIA – A REVIEW","authors":"Munibullah Munibullah, Yanmin Li, Kainat Munib, Zhidong Zhang","doi":"10.26873/svr-1464-2022","DOIUrl":"https://doi.org/10.26873/svr-1464-2022","url":null,"abstract":"Abstract: Peste des petits ruminants (PPR) is World Organization for Animal Health (OIE) notifiable, economically important transboundary, highly contagious, and an acute viral disease of small ruminants. The disease is caused by the PPR virus (PPRV). PPRV belongs to the genus Morbillivirus of the family Paramyxoviridae. The recent epidemiological and molecular characterization of PPR virus isolates subdivides them into four genetically distinct lineages (I, II, III and IV). The disease is endemic across Asia, the Middle East and African regions and is considered a major obstacle to the development of sustainable agriculture across the developing world due to its huge burden on the economy and development of the affected countries and has recently been targeted by the Food and Agriculture Organization (FAO) and the OIE for global eradication by 2030. PPR-endemic countries should join the regional force, and implement regional road maps for the progressive and successful control and elimination of PPRV. In this review, the regional epidemiology of PPR outbreaks and associated risk factors, including animal factors (age, species and sex), environmental factors (season, spatial distribution of disease in various locations) and trade associated factors with special reference to the PPR affected countries in South, Central and East Asia are comprehensively discussed.Key words: epidemiology; PPR-virus; risk factors; control; eradication; AsiaREGIONALNA EPIDEMIOLOGIJA IN Z NJO POVEZANI DEJAVNIKI TVEGANJA ZA KUGO DROBNICE V AZIJI – PREGLEDIzvleček: Kuga drobnice (angl., Peste des petits ruminants, PPR) je gospodarsko pomembna, čezmejno nevarna, visoko nalezljiva, akutna virusna bolezen drobnice, ki jo je potrebno prijaviti Svetovni organizaciji za zdravje živali (OIE). Bolezen povzroča virus PPR (PPRV). PPRV spada v rod virusov Morbillivirus iz družine Paramyxoviridae. Nedavna epidemiološka in molekularna karakterizacija izolatov virusa PPRV deli izolate na štiri genetsko različne linije (I, II, III in IV). Bolezen je endemična v Aziji, na bližnjem vzhodu in v Afriki. Zaradi močnega bremena za gospodarstvo in razvoj prizadetih držav velja za glavno oviro pri razvoju trajnostnega kmetijstva v državah v razvoju. Organizacija Združenih narodov za prehrano in kmetijstvo (FAO) in OIE sta nedavno določili, da je bolezen potrebno do leta 2030 izkoreniniti po vsem svetu. Države z endemijo PPR bi se morale pridružiti regionalnim silam in izvajati regionalne načrte za postopen in uspešen nadzor in izkoreninjenje PPRV. V tem pregledu so izčrpno obravnavani regionalna epidemiologija izbruhov PPR in povezani dejavniki tveganja, vključno z dejavniki živali (starost, vrsta in spol), okolja (letni čas, prostorska razširjenost bolezni na različnih lokacijah) in trgovine, s posebnim poudarkom na državah s PPR v južni, osrednji in vzhodni Aziji.Ključne besede: epidemiologija; virus PPR; dejavniki tveganja; nadzor; izkoreninjenje; Azija","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46767179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract: The extinct cave bear (CB) is often depicted as a large heavy animal with a prominent, massive skull and severely shortened pelvic limbs. The size and robustness of the CB are comparable to those of the largest living ursids. Great similarities between CB and the brown bear (BB) species prompted our morphometric comparison. The goal of this study was to elucidate the potential differences in the morphometric skull characteristics of CB and BB species. Craniometric measurements were performed on the skulls of both bear species and were compared to identify craniometric features indicative of possible adaptations in both bear species. The results revealed a marked difference in skull size; however, the shape of the CB cranium is generally known to be similar to that of the BB. In CB, the total length of the skull was approximately 1.5 times longer, and the external bony nasal aperture was larger due to the relatively shorter nasal bones. The nose length and median palate length were relatively longer in the CB, and the infraorbital foramen was located more caudally and closer to the zygomatic process. The infraorbital channel of the CB was located over to the second superior molar roots whereas in BB the latter extends beyond the roots of the first to the second superior molar roots. A marked difference was the non-existence of the three most anterior premolars in the maxilla and mandible of the CB, Higher body of the mandible, which, together with the extensive biting surfaces of the cheeks teeth, indicates a predominantly plant-based diet of the CB. The braincase length was considerably shorter in the CB, resulting in a relatively small neurocranium volume. The pronounced frontal fossa in the CB skull continues caudally into a strongly developed frontal area, which gives the CB skull a prominent steep profile. In summary, comparative craniometry showed that CB had a smaller neurocranial volume and had herbivore-adapted jaws and teeth. These metric features of the head skeleton may be related to a lower adaptability to extreme climatic conditions to which they were exposed to during the last Pleistocene glacial period, which may have contributed to their extinction. Key words: head skeleton; skull; mandible; craniometrical features; bearMORFOMETRIČNE ZNAČILNOSTI SKELETA GLAVE JAMSKEGA IN RJAVEGA MEDVEDA: PRIMERJALNA ŠTUDIJAIzvleček: Izumrli jamski medved je pogosto upodobljen kot robustna, težka žival z masivno lobanjo in izrazito skrajšanima medeničnima okončinama. Po velikosti ga lahko primerjamo z največjimi vrstami današnjih medvedov. Domnevne podobnosti med jamskim in rjavim medvedom so spodbudile našo primerjalno študijo, s katero želimo pojasniti morebitne razlike v morfometričnih značilnostih lobanj, iz česar je mogoče sklepati na možne prilagoditve pri obeh vrstah. Oblika lobanje je pri jamskem medvedu precej podobna obliki pri rjavem medvedu, ugotovili pa smo izrazito razliko v velikosti. Pri jamskem medvedu je celotna
{"title":"MORPHOMETRICAL FEATURES OF THE CAVE BEAR AND BROWN BEAR HEAD SKELETON: A COMPARATIVE STUDY","authors":"M. Uršič","doi":"10.26873/svr-1317-2022","DOIUrl":"https://doi.org/10.26873/svr-1317-2022","url":null,"abstract":"Abstract: The extinct cave bear (CB) is often depicted as a large heavy animal with a prominent, massive skull and severely shortened pelvic limbs. The size and robustness of the CB are comparable to those of the largest living ursids. Great similarities between CB and the brown bear (BB) species prompted our morphometric comparison. The goal of this study was to elucidate the potential differences in the morphometric skull characteristics of CB and BB species. Craniometric measurements were performed on the skulls of both bear species and were compared to identify craniometric features indicative of possible adaptations in both bear species. The results revealed a marked difference in skull size; however, the shape of the CB cranium is generally known to be similar to that of the BB. In CB, the total length of the skull was approximately 1.5 times longer, and the external bony nasal aperture was larger due to the relatively shorter nasal bones. The nose length and median palate length were relatively longer in the CB, and the infraorbital foramen was located more caudally and closer to the zygomatic process. The infraorbital channel of the CB was located over to the second superior molar roots whereas in BB the latter extends beyond the roots of the first to the second superior molar roots. A marked difference was the non-existence of the three most anterior premolars in the maxilla and mandible of the CB, Higher body of the mandible, which, together with the extensive biting surfaces of the cheeks teeth, indicates a predominantly plant-based diet of the CB. The braincase length was considerably shorter in the CB, resulting in a relatively small neurocranium volume. The pronounced frontal fossa in the CB skull continues caudally into a strongly developed frontal area, which gives the CB skull a prominent steep profile. In summary, comparative craniometry showed that CB had a smaller neurocranial volume and had herbivore-adapted jaws and teeth. These metric features of the head skeleton may be related to a lower adaptability to extreme climatic conditions to which they were exposed to during the last Pleistocene glacial period, which may have contributed to their extinction. Key words: head skeleton; skull; mandible; craniometrical features; bearMORFOMETRIČNE ZNAČILNOSTI SKELETA GLAVE JAMSKEGA IN RJAVEGA MEDVEDA: PRIMERJALNA ŠTUDIJAIzvleček: Izumrli jamski medved je pogosto upodobljen kot robustna, težka žival z masivno lobanjo in izrazito skrajšanima medeničnima okončinama. Po velikosti ga lahko primerjamo z največjimi vrstami današnjih medvedov. Domnevne podobnosti med jamskim in rjavim medvedom so spodbudile našo primerjalno študijo, s katero želimo pojasniti morebitne razlike v morfometričnih značilnostih lobanj, iz česar je mogoče sklepati na možne prilagoditve pri obeh vrstah. Oblika lobanje je pri jamskem medvedu precej podobna obliki pri rjavem medvedu, ugotovili pa smo izrazito razliko v velikosti. Pri jamskem medvedu je celotna ","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45854287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
U. Topaloğlu, M. Ketani, Mehmet Erdem Aakbalink, H. Saǧsöz, B. Güney Saruhan, B. Bayram
Abstract: In addition to its many functions, the placenta is characterized by the intensity and characteristics of its hormonal functions. It has been reported that the development and vascularization of the placenta with normal fetal growth is regulated by many factors such as growth factors, transcriptional factors, and homeobox proteins. This study was conducted to determine the distribution and possible physiological roles of HOXA11 and HLX in the uterus and placenta of pregnant cows. In this study, 27 pregnant Holstein breed cow uteri and placentas obtained from private slaughterhouses were used as tissue samples. In order to determine the pregnancy period of the obtained uterus, the ages of the fetuses were calculated according to the age calculation formula. The uteri were classified under three different groups as the first (69-89 days), second (99-178 days), and third (190-269 days) periods of pregnancy so that there are 9 animals in each group. Tissue samples were then subjected to immunohistochemistry to demonstrate the presence of HOXA11 and HLX proteins. It was observed that HOXA11 and HLX immunoreactions occurred prominently in the luminal and glandular epithelial cells of the uterus during pregnancy, and the maternal epithelial cells of the placenta and UTC and TGC/BNCs. In addition, it was determined that HOXA11 immunoreaction also formed in some vascular endothelial cells. Immunohistochemical findings in this study suggested that Hoxa11 and HLX may affect the proliferation and differentiation of cells in the uterus and placenta during pregnancy in cows, and that these proteins may contribute to normal uterine physiology, placentation formation, and continuity of pregnancy in cows.Key words: Hox proteins; HOXA11; HLX; placentaIMUNOLOKALIZACIJA PROTEINOV HOXA11 IN HLX V POSTELJICI KRAVE MED BREJOSTJOIzvleček: Za posteljico so poleg številnih funkcij značilne intenzivnost in karakteristike njenih hormonskih funkcij. Poročali so,da razvoj in ožiljenost posteljice pri normalni rasti ploda uravnavajo številni dejavniki, kot so rastni dejavniki, transkripcijski djavniki in homeoboks proteini. Ta študija je bila izvedena z namenom določiti porazdelitev in morebitno fiziološko vlogo protnov HOXA11 in HLX v maternici in posteljici brejih krav. Za vzorce tkiv smo uporabili maternice in posteljice 27 krav pasem Holstein, pridobljenih v zasebnih klavnicah. Obdobje brejosti smo izračunali na podlagi starosti plodov po formuli. Maternice smoglede na obdobje brejosti uvrstili v tri skupine po 9 vzorcev: 1 (69-89 dni), 2 (99-178 dni) in 3 (190-269 dni). Prisotnost proteinoHOXA11 in HLX v vzorcih tkiv smo preverili z imunohistokemijo. Proteini HOXA11 in HLX so bili prisotni predvsem v luminalnihin žleznih epitelijskih celicah maternice ter v epitelijskih celicah maternalne posteljice, UTC in TGC/BNC. Prisotnost HOXA1je bila zaznavna tudi v nekaterih žilnih endotelijskih celicah. Rezultati imunohistokemije v tej študiji kažejo na morebiten
摘要:胎盘除了具有多种功能外,还具有其激素功能的强度和特点。据报道,正常胎儿生长的胎盘的发育和血管形成受多种因素的调控,如生长因子、转录因子和同源盒蛋白。本研究旨在探讨HOXA11和HLX在奶牛子宫和胎盘中的分布及其可能的生理作用。本研究以27头来自私营屠宰场的荷斯坦奶牛妊娠子宫和胎盘为组织样本。为了确定获得的子宫的妊娠期,根据年龄计算公式计算胎儿的年龄。将子宫分为妊娠第一期(69 ~ 89天)、妊娠第二期(99 ~ 178天)、妊娠第三期(190 ~ 269天)3组,每组9只。然后对组织样本进行免疫组化以证明HOXA11和HLX蛋白的存在。结果发现,妊娠期子宫腔上皮细胞、腺上皮细胞、胎盘母细胞、UTC和TGC/ bnc中均显著发生HOXA11和HLX免疫反应。此外,我们还确定在一些血管内皮细胞中也形成了HOXA11免疫反应。本研究免疫组化结果提示,Hoxa11和HLX可能影响奶牛妊娠期间子宫和胎盘细胞的增殖和分化,这些蛋白可能有助于奶牛正常的子宫生理、胎盘形成和妊娠的持续。关键词:Hox蛋白;HOXA11;HLX;placentaIMUNOLOKALIZACIJA蛋白HOXA11 IN HLX V posteljci KRAVE MED brejostjoizvle ek: Za posteljico so poleg številnih funkcij zna ilne intenzivnost IN karakterislike njenih hrskh funkcij。poroali so,da razvoj in ožiljenost posteljice pri normalni rasti ploda uravnavajo številni dejavniki, kot so rastni dejavniki, transkripcijski djavniki in homebooks proteini。那študija je bila izvedena z namenom dolo i porazdelitev in morebitno fiziološko vlogo pronov HOXA11 in HLX v maternici in posteljici brejih krav。27日,在克拉夫帕西姆·荷尔斯泰因,克拉夫帕西姆·克拉夫尼察,克拉夫尼察,克拉夫尼察。Obdobje brejosti smo izraunali na podlagi starosti plodov po formuli。母鼠smoglde na obdobje brejosti uvrstili v tri skupine po 9 vzorcev: 1 (69-89 dni), 2 (99-178 dni), 3 (190-269 dni)。hxxa11蛋白在hxxv病毒中的表达及其在免疫组织中的作用。蛋白HOXA11在HLX中表达,因此,bili prispte - predvin和luminalniin分别为žleznih上皮细胞母细胞前和上皮细胞母细胞后,UTC在TGC/BNC中表达。HOXA1je bila zaznavna tudi v nekaterih žilnih endotelijskih celicah。研究结果表明:免疫组织研究表明:hxx蛋白HOXA11在hxx细胞中的增殖能力,不同细胞间的增殖能力,不同细胞间的增殖能力,不同细胞间的增殖能力,不同细胞间的增殖能力,不同细胞间的增殖能力,不同细胞间的增殖能力,不同细胞间的增殖能力,不同细胞间的增殖能力,不同细胞间的增殖能力。klju nebeede:蛋白质Hox;HOXA11;HLX;posteljica
{"title":"IMMUNOLOCALIZATION OF HOXA11 AND HLX PROTEINS IN COW PLACENTA DURING PREGNANCY","authors":"U. Topaloğlu, M. Ketani, Mehmet Erdem Aakbalink, H. Saǧsöz, B. Güney Saruhan, B. Bayram","doi":"10.26873/svr-1347-2022","DOIUrl":"https://doi.org/10.26873/svr-1347-2022","url":null,"abstract":"Abstract: In addition to its many functions, the placenta is characterized by the intensity and characteristics of its hormonal functions. It has been reported that the development and vascularization of the placenta with normal fetal growth is regulated by many factors such as growth factors, transcriptional factors, and homeobox proteins. This study was conducted to determine the distribution and possible physiological roles of HOXA11 and HLX in the uterus and placenta of pregnant cows. In this study, 27 pregnant Holstein breed cow uteri and placentas obtained from private slaughterhouses were used as tissue samples. In order to determine the pregnancy period of the obtained uterus, the ages of the fetuses were calculated according to the age calculation formula. The uteri were classified under three different groups as the first (69-89 days), second (99-178 days), and third (190-269 days) periods of pregnancy so that there are 9 animals in each group. Tissue samples were then subjected to immunohistochemistry to demonstrate the presence of HOXA11 and HLX proteins. It was observed that HOXA11 and HLX immunoreactions occurred prominently in the luminal and glandular epithelial cells of the uterus during pregnancy, and the maternal epithelial cells of the placenta and UTC and TGC/BNCs. In addition, it was determined that HOXA11 immunoreaction also formed in some vascular endothelial cells. Immunohistochemical findings in this study suggested that Hoxa11 and HLX may affect the proliferation and differentiation of cells in the uterus and placenta during pregnancy in cows, and that these proteins may contribute to normal uterine physiology, placentation formation, and continuity of pregnancy in cows.Key words: Hox proteins; HOXA11; HLX; placentaIMUNOLOKALIZACIJA PROTEINOV HOXA11 IN HLX V POSTELJICI KRAVE MED BREJOSTJOIzvleček: Za posteljico so poleg številnih funkcij značilne intenzivnost in karakteristike njenih hormonskih funkcij. Poročali so,da razvoj in ožiljenost posteljice pri normalni rasti ploda uravnavajo številni dejavniki, kot so rastni dejavniki, transkripcijski djavniki in homeoboks proteini. Ta študija je bila izvedena z namenom določiti porazdelitev in morebitno fiziološko vlogo protnov HOXA11 in HLX v maternici in posteljici brejih krav. Za vzorce tkiv smo uporabili maternice in posteljice 27 krav pasem Holstein, pridobljenih v zasebnih klavnicah. Obdobje brejosti smo izračunali na podlagi starosti plodov po formuli. Maternice smoglede na obdobje brejosti uvrstili v tri skupine po 9 vzorcev: 1 (69-89 dni), 2 (99-178 dni) in 3 (190-269 dni). Prisotnost proteinoHOXA11 in HLX v vzorcih tkiv smo preverili z imunohistokemijo. Proteini HOXA11 in HLX so bili prisotni predvsem v luminalnihin žleznih epitelijskih celicah maternice ter v epitelijskih celicah maternalne posteljice, UTC in TGC/BNC. Prisotnost HOXA1je bila zaznavna tudi v nekaterih žilnih endotelijskih celicah. Rezultati imunohistokemije v tej študiji kažejo na morebiten ","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42458940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract: Several studies have implicated reactive oxygen species in perpetuation of inflammation and subsequent destruction of joints in patients with arthritis. Antioxidant effects of Buchholzia coriacea ethanol leaf-extract, aqueous and ethyl acetate fractions on oxidative stress indices in rheumatoid arthritic female wistar albino rats were examined. 180 rats were randomly divided into 12 groups. Fifteen rats were placed in each group. Rats without arthritis were placed in Group 1. Rheumatoid arthritis was induced in groups 2 to 12 by intradermal injection of 0.1 ml complete Freund’s adjuvant into the left hind paws of rats. Group 2 (arthritic rats) did not receive any treatment but rather were given normal saline while group 3 (arthritic rats) received 5 mg/kg indomethacinTM (a standard drug). Rats in Groups 4 to 12 were administered the samples at doses of 200, 400 and 800 mg/kg body weight. Freund’s adjuvant administration led to inflammation and oxidative stress which were marked by significant (P0.05) increase in paw sizes, oxidative stress markers and reduced body weight of the rats. Arthritic rats were treated with standard drug and samples (at varied doses) and this resulted to reversal of the trend of those parameters in a time and dose-dependent manner. Rats that received 800 mg/kg of the aqueous fraction displayed the best desirable result which was similar to the effect of indomethacin. Thus, Buchholzia coriacea ethanol extract and fractions may be useful in the management of oxidative stress which is very common among individuals with rheumatoid arthritis.Key words: rheumatoid arthritis; Buchholzia coriacea; free radicals; oxidative stress; reactive oxygen speciesANTIOKSIDATIVNI UČINEK ETANOLNEGA IZVLEČKA IN FRAKCIJ LISTOV Buchholzia coriacea NA FREUNDOV ADJUVANTNI ARTRITIS PRI ALBINO PODGANAH: PRIMERJALNA ŠTUDIJAIzvleček: V več študijah je bil nakazan pomen reaktivnih kisikovih zvrsti pri ohranjanju vnetja in poznejšem uničenju sklepov pri pacientih z osteoartritisom. Proučevali smo antioksidantivne učinke etanolovega izvlečka ter vodnih in etil acetatnih frakcij listov Buchholzia coriacea na kazalce oksidativnega stresa pri samicah albino podgan Wistar z revmatoidnih artritisom. 180 podgan smo naključno porazdelili v 12 skupin. V vsaki skupini je bilo 15 podgan. Podgane brez artritisa so bile uvrščene v skupino 1. V skupinah 2 do 12 so bile podgane z revmatoidnim artritisom, povzročenim z intradermalno injekcijo 0,1 ml Freundovega kompletnega adjuvansa v zadnjo levo taco podgan. Skupina 2 (podgane z artritisom) ni bila zdravljena, prejela je fiziološko raztopino, skupina 3 (podgane z artritisom) pa je prejela 5 mg/kg indometacinaTM (standardno zdravilo). Podgane v skupinah 4 do 12 so prejele vzorce adjuvansa v odmerkih 200, 400 in 800 mg/kg telesne mase. Aplikacija Freundovega adjuvansa je povzročila vnetje in oksidativni stres, kar se je kazalo v značilnem (p0.05) povišanju velikosti tac in kazalcev oksidativnega stresa ter
{"title":"ANTIOXIDANT EFFECT OF Buchholzia coriacea ETHANOL LEAF-EXTRACT AND FRACTIONS ON FREUND’S ADJUVANT-INDUCED ARTHRITIS IN ALBINO RATS: A COMPARATIVE STUDY","authors":"E. Alum","doi":"10.26873/svr-1150-2022","DOIUrl":"https://doi.org/10.26873/svr-1150-2022","url":null,"abstract":"Abstract: Several studies have implicated reactive oxygen species in perpetuation of inflammation and subsequent destruction of joints in patients with arthritis. Antioxidant effects of Buchholzia coriacea ethanol leaf-extract, aqueous and ethyl acetate fractions on oxidative stress indices in rheumatoid arthritic female wistar albino rats were examined. 180 rats were randomly divided into 12 groups. Fifteen rats were placed in each group. Rats without arthritis were placed in Group 1. Rheumatoid arthritis was induced in groups 2 to 12 by intradermal injection of 0.1 ml complete Freund’s adjuvant into the left hind paws of rats. Group 2 (arthritic rats) did not receive any treatment but rather were given normal saline while group 3 (arthritic rats) received 5 mg/kg indomethacinTM (a standard drug). Rats in Groups 4 to 12 were administered the samples at doses of 200, 400 and 800 mg/kg body weight. Freund’s adjuvant administration led to inflammation and oxidative stress which were marked by significant (P0.05) increase in paw sizes, oxidative stress markers and reduced body weight of the rats. Arthritic rats were treated with standard drug and samples (at varied doses) and this resulted to reversal of the trend of those parameters in a time and dose-dependent manner. Rats that received 800 mg/kg of the aqueous fraction displayed the best desirable result which was similar to the effect of indomethacin. Thus, Buchholzia coriacea ethanol extract and fractions may be useful in the management of oxidative stress which is very common among individuals with rheumatoid arthritis.Key words: rheumatoid arthritis; Buchholzia coriacea; free radicals; oxidative stress; reactive oxygen speciesANTIOKSIDATIVNI UČINEK ETANOLNEGA IZVLEČKA IN FRAKCIJ LISTOV Buchholzia coriacea NA FREUNDOV ADJUVANTNI ARTRITIS PRI ALBINO PODGANAH: PRIMERJALNA ŠTUDIJAIzvleček: V več študijah je bil nakazan pomen reaktivnih kisikovih zvrsti pri ohranjanju vnetja in poznejšem uničenju sklepov pri pacientih z osteoartritisom. Proučevali smo antioksidantivne učinke etanolovega izvlečka ter vodnih in etil acetatnih frakcij listov Buchholzia coriacea na kazalce oksidativnega stresa pri samicah albino podgan Wistar z revmatoidnih artritisom. 180 podgan smo naključno porazdelili v 12 skupin. V vsaki skupini je bilo 15 podgan. Podgane brez artritisa so bile uvrščene v skupino 1. V skupinah 2 do 12 so bile podgane z revmatoidnim artritisom, povzročenim z intradermalno injekcijo 0,1 ml Freundovega kompletnega adjuvansa v zadnjo levo taco podgan. Skupina 2 (podgane z artritisom) ni bila zdravljena, prejela je fiziološko raztopino, skupina 3 (podgane z artritisom) pa je prejela 5 mg/kg indometacinaTM (standardno zdravilo). Podgane v skupinah 4 do 12 so prejele vzorce adjuvansa v odmerkih 200, 400 in 800 mg/kg telesne mase. Aplikacija Freundovega adjuvansa je povzročila vnetje in oksidativni stres, kar se je kazalo v značilnem (p0.05) povišanju velikosti tac in kazalcev oksidativnega stresa ter ","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49376708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Deveci, Z. Yurtal, C. İşler, Sara Busra Emiroglu, İbrahim Alakuş, M. Altuğ
Abstract: The aim of this study was to evaluate the surgical outcome and mortality of diaphragmatic hernia in cats and present herniorrhaphy results. Diagnostic and surgical data from 25 cats with diaphragmatic hernias were analyzed retrospectively. The cats were between the ages of 2 months and 4 years, included 9 cases of males (5/9 neutered) and 16 cases of females (9/16 spayed). acute cases were 12/25 and chronic cases were 13/25. Diaphragmatic rupture locations were defined according to the points that are determined based on a clockwise scale. Location was classified as multiple in 10 (40 %), ventral in 7 (28 %), right in 5 (20 %), and left in 3 (12 %) cases. The three most frequently herniated organs were small intestine (20, 80 %), liver (16, 64 %), and omentum (15, 60 %). Postoperative survival rates were 83.3 % for acute cases and 69.2 % for chronic. Full recovery was achieved in 19 (76 %) of the cats. Results suggest that the location and size of diaphragm ruptures, the organs herniated, and the presence of concurrent pathologies directly affect mortality. Mortality is higher in cases with concurrent injuries, large diaphragmatic ruptures, and excessively herniated organs.Key words: prognosis; feline diseases; hernia; rupture; survivalHERNIORAFIJA IN KIRURŠKI IZIDI DIAFRAGMALNE KILE PRI MAČKAHIzvleček: Namen te študije je bil ovrednotiti kirurški izid in smrtnost diafragmalne kile pri mačkah ter predstaviti rezultate herniorafije. Retrospektivno smo analizirali diagnostične in kirurške podatke pri 25 mačkah z diafragmalno kilo. Mačke so bile stare od 2 mesecev do 4 let, med njimi je bilo 9 samcev (5/9 kastriranih) in 16 samic (9/16 steriliziranih). Akutnih primerov je bilo 12/25 in kroničnih 13/25. Mesta pretrganja diafragme so bila opredeljena glede na točke, določene na podlagi lestvice v smeri urinega kazalca. Pretrganje je bilo določeno kot večmestno v 10 (40 %) primerih, ventralno v 7 (28 %), desno v 5 (20 %) in levo v 3 (12 %) primerih. Trije najpogosteje vrinjeni organi so bili tanko črevo (20, 80 %), jetra (16, 64 %) in omentum (15, 60 %). Preživetje po operaciji je bilo pri akutnih primerih 83,3 %, pri kroničnih pa 69,2 %. Popolno okrevanje je bilo doseženo pri 19 (76 %) mačkah. Rezultati kažejo, da na umrljivost neposredno vplivajo mesto in velikost raztrganine diafragme, vrinjeni organi in prisotnost sočasnih patologij. Smrtnost je večja pri sočasnih poškodbah, velikih raztrganinah diafragme in prekomernem vrinjenju organov.Ključne besede: prognoza; bolezni mačk; kila; raztrganina; preživetje
摘要:本研究的目的是评估猫膈疝的手术效果和死亡率,并介绍疝修补术的结果。回顾性分析25只猫膈疝的诊断和手术资料。这些猫的年龄在2个月至4岁之间,其中9只公猫(5/9已绝育)和16只母猫(9/16已绝育)。急性病例12/25,慢性病例13/25。根据按顺时针刻度确定的点来定义横膈膜破裂位置。10例为多发(40%),腹侧7例(28%),右侧5例(20%),左侧3例(12%)。最常见的三个器官是小肠(20.80%)、肝脏(16.64%)和网膜(15.60%)。急性组术后生存率为83.3%,慢性组术后生存率为69.2%。19只(76%)猫完全康复。结果提示,膈膜破裂的位置、大小、器官疝出、并发病变的存在直接影响死亡率。并发损伤、大膈破裂和器官过度突出的病例死亡率更高。关键词:预后;猫科动物疾病;疝;破裂;survivalHERNIORAFIJA IN KIRURŠKI IZIDI DIAFRAGMALNE KILE PRI MAČKAHIzvleček: Namen the študije je bil overrednotiti kirurški izid IN smartnost DIAFRAGMALNE KILE PRI makah后prestaviti调节herniorafije。回顾性分析与分析诊断研究了kirurške podatke pri 25 makaz diafragmalno kg中的ne。make so bile stare od 2 mesecev do 4 let, med njimi je billion 9 samcev (5/9 kastrriranih) in 16 samcev (9/16 steriliziranih)。Akutnih primerov je bilo 12/25 in kroninih 13/25。Mesta pregenja diafragme so bila prepredeljena glede de de ke, doloene na podlagi lestvice v smeri urinega kazalca。Pretrganje je bilo dolo eno kot ve mestno v 10 (40%) primerih, ventralno v 7 (28%), desno v 5 (20%) in levo v 3 (12%) primerih。Trije najpogosteje vrinjeni organi so bili tanko revo (20.80%), jetra (16.64%) in omentum(15.60%)。Preživetje po operaciji je bilo pri akutnih primerih 83,3%, pri kroni nih pa 69,2%。Popolno okrevanje je bilo doseženo pri 19 (76%) ma kah。Rezultati kažejo, da na umrjivost neposredno vplivajo mesto velikost razgine图,vrinjeni organis most so asnih病理学。[3] [j] [j] [j] [j] [j] [j] [j]。klju9 . nebeede:预后;马bolezničk;kila;raztrganina;preživetje
{"title":"HERNIORRHAPHY AND SURGICAL OUTCOMES OF DIAPHRAGMATIC HERNIA IN CATS","authors":"M. Deveci, Z. Yurtal, C. İşler, Sara Busra Emiroglu, İbrahim Alakuş, M. Altuğ","doi":"10.26873/svr-1295-2022","DOIUrl":"https://doi.org/10.26873/svr-1295-2022","url":null,"abstract":"Abstract: The aim of this study was to evaluate the surgical outcome and mortality of diaphragmatic hernia in cats and present herniorrhaphy results. Diagnostic and surgical data from 25 cats with diaphragmatic hernias were analyzed retrospectively. The cats were between the ages of 2 months and 4 years, included 9 cases of males (5/9 neutered) and 16 cases of females (9/16 spayed). acute cases were 12/25 and chronic cases were 13/25. Diaphragmatic rupture locations were defined according to the points that are determined based on a clockwise scale. Location was classified as multiple in 10 (40 %), ventral in 7 (28 %), right in 5 (20 %), and left in 3 (12 %) cases. The three most frequently herniated organs were small intestine (20, 80 %), liver (16, 64 %), and omentum (15, 60 %). Postoperative survival rates were 83.3 % for acute cases and 69.2 % for chronic. Full recovery was achieved in 19 (76 %) of the cats. Results suggest that the location and size of diaphragm ruptures, the organs herniated, and the presence of concurrent pathologies directly affect mortality. Mortality is higher in cases with concurrent injuries, large diaphragmatic ruptures, and excessively herniated organs.Key words: prognosis; feline diseases; hernia; rupture; survivalHERNIORAFIJA IN KIRURŠKI IZIDI DIAFRAGMALNE KILE PRI MAČKAHIzvleček: Namen te študije je bil ovrednotiti kirurški izid in smrtnost diafragmalne kile pri mačkah ter predstaviti rezultate herniorafije. Retrospektivno smo analizirali diagnostične in kirurške podatke pri 25 mačkah z diafragmalno kilo. Mačke so bile stare od 2 mesecev do 4 let, med njimi je bilo 9 samcev (5/9 kastriranih) in 16 samic (9/16 steriliziranih). Akutnih primerov je bilo 12/25 in kroničnih 13/25. Mesta pretrganja diafragme so bila opredeljena glede na točke, določene na podlagi lestvice v smeri urinega kazalca. Pretrganje je bilo določeno kot večmestno v 10 (40 %) primerih, ventralno v 7 (28 %), desno v 5 (20 %) in levo v 3 (12 %) primerih. Trije najpogosteje vrinjeni organi so bili tanko črevo (20, 80 %), jetra (16, 64 %) in omentum (15, 60 %). Preživetje po operaciji je bilo pri akutnih primerih 83,3 %, pri kroničnih pa 69,2 %. Popolno okrevanje je bilo doseženo pri 19 (76 %) mačkah. Rezultati kažejo, da na umrljivost neposredno vplivajo mesto in velikost raztrganine diafragme, vrinjeni organi in prisotnost sočasnih patologij. Smrtnost je večja pri sočasnih poškodbah, velikih raztrganinah diafragme in prekomernem vrinjenju organov.Ključne besede: prognoza; bolezni mačk; kila; raztrganina; preživetje","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43647484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}