Pub Date : 2022-07-01DOI: 10.21608/svu.2022.136479.1197
A. Hassan, Z. Al-Amgad, M. Youssef
Competing Abstract Chronic restraint stress (CRS) immobilize animal and induces the production of oxidative radicals as a preliminary cause of various health complications. CRS-induced oxidative stress led to pathological changes in brain tissue and consequently the general body condition. Thus, we elucidated the potential protective impact of L-Arginine (L-Arg), conditional amino acid and essential source of nitric oxide, on oxidative stress and brain tissue. BALB/c mice were randomly divided into five groups (n = 6) that received either 1) daily intraperitoneal (I.P) injection of phosphate-buffered saline (PBS) (Control group) (0.5ml), 2) I.P injection of PBS in combination with CRS (2h/day for 10 consecutive days) (CRS group), 3) I.P injection of L-Arg (500 mg/kg b.wt) with CRS (L-Arg + CRS group), 4) L-Arg alone (L-Arg group), or 5) pretreatment with I.P injection of L-N G –nitro-L-arginine methyl ester (l-NAME) (30 mg/kg b.wt), a potent common inhibitor of nitric oxide synthase (NOS), 30 minutes before L-Arg injection and CRS application (L-Arg + L-NAME + CRS group). Total antioxidant capacity (T-AOC), catalase, and histopathological status of brain tissue were evaluated. CRS significantly suppressed the T-AOC compared to the control. Furthermore, L-Arg elevated the T-AOC whereas L-NAME significantly reversed the action of L-Arg on CRS-induced T-AOC. L-Arg could not rescue CRS-decreased catalase. It is concluded that NOS is an essential mediator of the L-Arg ameliorative effect on CRS-induced oxidative stress in serum and brain tissue. Moreover, L-Arg antioxidant action is not mediated by catalase.
{"title":"Nitric oxide synthase mediates the antioxidant effect of L-Arginine in the brain of mice subjected to chronic restrain stress","authors":"A. Hassan, Z. Al-Amgad, M. Youssef","doi":"10.21608/svu.2022.136479.1197","DOIUrl":"https://doi.org/10.21608/svu.2022.136479.1197","url":null,"abstract":"Competing Abstract Chronic restraint stress (CRS) immobilize animal and induces the production of oxidative radicals as a preliminary cause of various health complications. CRS-induced oxidative stress led to pathological changes in brain tissue and consequently the general body condition. Thus, we elucidated the potential protective impact of L-Arginine (L-Arg), conditional amino acid and essential source of nitric oxide, on oxidative stress and brain tissue. BALB/c mice were randomly divided into five groups (n = 6) that received either 1) daily intraperitoneal (I.P) injection of phosphate-buffered saline (PBS) (Control group) (0.5ml), 2) I.P injection of PBS in combination with CRS (2h/day for 10 consecutive days) (CRS group), 3) I.P injection of L-Arg (500 mg/kg b.wt) with CRS (L-Arg + CRS group), 4) L-Arg alone (L-Arg group), or 5) pretreatment with I.P injection of L-N G –nitro-L-arginine methyl ester (l-NAME) (30 mg/kg b.wt), a potent common inhibitor of nitric oxide synthase (NOS), 30 minutes before L-Arg injection and CRS application (L-Arg + L-NAME + CRS group). Total antioxidant capacity (T-AOC), catalase, and histopathological status of brain tissue were evaluated. CRS significantly suppressed the T-AOC compared to the control. Furthermore, L-Arg elevated the T-AOC whereas L-NAME significantly reversed the action of L-Arg on CRS-induced T-AOC. L-Arg could not rescue CRS-decreased catalase. It is concluded that NOS is an essential mediator of the L-Arg ameliorative effect on CRS-induced oxidative stress in serum and brain tissue. Moreover, L-Arg antioxidant action is not mediated by catalase.","PeriodicalId":22190,"journal":{"name":"SVU-International Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48345812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-01DOI: 10.21608/svu.2022.139448.1202
N. Abdel-Aziz, Alshaima Hassanien
This study detected the presence of antimicrobial resistance L. monocytogenes in meat and poultry products as minced meat, luncheon, and frozen chicken fillet sold in Sohag city markets. Also, study the effect of two essential oils (EOs) like clove and thyme on the isolated strains of L. monocytogenes . Bacteriological culture and PCR were used for L. monocytogenes identification in 195 meat and poultry products samples such as minced meat, luncheon, and frozen chicken fillet (65 each). Fourteen antimicrobials were tested against L. monocytogenes using disk diffusion method. Clove and thyme EOs were used at nine concentrations (100%, 50%, 25%, 12.5%, 6. 25%, 3. 125%, 1.56%, 0.78%, and 0.39%) to detect their antibacterial effect using well diffusion method. L. monocytogenes were detected in 6 (3.1%) out of 195 meat and poultry samples. Minced meat harbors the highest infection rate, followed by luncheon and frozen chicken fillet with percentage of 4.6%, 3.1%, and 1.53%, respectively. Most of L. monocytogenes isolates were resistant to several antimicrobial from varied groups such as streptomycin, tetracycline, ampicillin, cefotaxime, ceftriaxone, vancomycin, and amikacin. Clove and thyme EOs have inhibitory action on L. monocytogenes growth which significantly increased with concentration where the minimum inhibitory concentration was 3.125% for clove essential oil (EO), and 1.56% for thyme EO. The inhibitory action of Clove and thyme EOs enables them to be used in food industry as antibacterial to increase the products shelf life.
{"title":"The impact of Clove and thyme essential oils on Listeria monocytogenes isolated from meat and poultry products","authors":"N. Abdel-Aziz, Alshaima Hassanien","doi":"10.21608/svu.2022.139448.1202","DOIUrl":"https://doi.org/10.21608/svu.2022.139448.1202","url":null,"abstract":"This study detected the presence of antimicrobial resistance L. monocytogenes in meat and poultry products as minced meat, luncheon, and frozen chicken fillet sold in Sohag city markets. Also, study the effect of two essential oils (EOs) like clove and thyme on the isolated strains of L. monocytogenes . Bacteriological culture and PCR were used for L. monocytogenes identification in 195 meat and poultry products samples such as minced meat, luncheon, and frozen chicken fillet (65 each). Fourteen antimicrobials were tested against L. monocytogenes using disk diffusion method. Clove and thyme EOs were used at nine concentrations (100%, 50%, 25%, 12.5%, 6. 25%, 3. 125%, 1.56%, 0.78%, and 0.39%) to detect their antibacterial effect using well diffusion method. L. monocytogenes were detected in 6 (3.1%) out of 195 meat and poultry samples. Minced meat harbors the highest infection rate, followed by luncheon and frozen chicken fillet with percentage of 4.6%, 3.1%, and 1.53%, respectively. Most of L. monocytogenes isolates were resistant to several antimicrobial from varied groups such as streptomycin, tetracycline, ampicillin, cefotaxime, ceftriaxone, vancomycin, and amikacin. Clove and thyme EOs have inhibitory action on L. monocytogenes growth which significantly increased with concentration where the minimum inhibitory concentration was 3.125% for clove essential oil (EO), and 1.56% for thyme EO. The inhibitory action of Clove and thyme EOs enables them to be used in food industry as antibacterial to increase the products shelf life.","PeriodicalId":22190,"journal":{"name":"SVU-International Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43786058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-01DOI: 10.21608/svu.2022.145199.1207
Z. Ahmed, Huda Elsayed, Nady Albarbary
Quail meat is a delicate white game meat with extremely low skin fat and low cholesterol value. It is rich in micronutrients and vitamins including vitamin B6, niacin, thiamin, pantothenic acid and riboflavin, folate and vitamin E and K. It is therefore recommended for people with high cholesterol levels. Fifty random samples were collected from different restaurants in Luxor and Aswan cities, Egypt to evaluate the nitrogen (TVB-N mg/100gm), and Thiobarbituric acid (TBA mg/Kg) were 5.9±0.01, and 12.1±0.2 and 0.72±0.03, respectively.
{"title":"Quality assessment and detection of multiple drug-resistant food-borne aerobic bacteria in frozen quail in Luxor and Aswan city","authors":"Z. Ahmed, Huda Elsayed, Nady Albarbary","doi":"10.21608/svu.2022.145199.1207","DOIUrl":"https://doi.org/10.21608/svu.2022.145199.1207","url":null,"abstract":"Quail meat is a delicate white game meat with extremely low skin fat and low cholesterol value. It is rich in micronutrients and vitamins including vitamin B6, niacin, thiamin, pantothenic acid and riboflavin, folate and vitamin E and K. It is therefore recommended for people with high cholesterol levels. Fifty random samples were collected from different restaurants in Luxor and Aswan cities, Egypt to evaluate the nitrogen (TVB-N mg/100gm), and Thiobarbituric acid (TBA mg/Kg) were 5.9±0.01, and 12.1±0.2 and 0.72±0.03, respectively.","PeriodicalId":22190,"journal":{"name":"SVU-International Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46893758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-01DOI: 10.21608/svu.2022.131641.1190
Basma J. Ahmed, L. El-Malt, Karima G. Abdel Hameed, Mona A El-Zamkan
Listeria spp . is a very resilient bacteria that can persist hostile dairy-processing environments and consequently, food safety challenges appear here with respect to control its growth. L. monocytogenes is the most important species that cause a great concern in both food processing and public health. Therefore, this study was performed to investigate the incidence of Listeria spp. in 450 samples of raw milk, pasteurized milk, flavored milk, dominate cheese, kareish cheese, small scale ice cream, large scale ice cream, small scale yoghurt, and large scale yoghurt (50 each) were collected from dairy farms, local dairy shops and street vendors in Qena city, Egypt. Listeria spp. were isolated from 27 (6%) out of the total samples examined. They were recovered from the tested market raw milk, kareish cheese, small scale ice cream and domiati cheese samples with percentages of 20, 16, 14% and 4%, respectively. However, Listeria spp. was absent in other analyzed samples. The most prevalent species was L. monocytogenes 10 (2.22%), followed by L. grayii 9 (2%), L. welshimeri 4 (0.89%) and L. innocua 3 (0.67%) with an overall 5.7% verified by PCR. Eight out of 10 L. monocytogenes isolates were confirmed by the presence of the 16S rRNA gene that implies a risk of food-borne listeriosis among dairy product consumers. Furthermore, a unique approach was used for the assessment of antibacterial activity of different concentrations of gentamicin as a reference antibiotic standard and hydroalcoholic coffee extract (H. A. coffee extract) (1, 3, 10 and 30 µg/ml) against local isolated L. monocytogenes strain in vitro using a microplate in ELISA reader is provided in the current work. The most Potent antimicrobial activity of gentamicin and H. A. coffee extract was obtained at 30 µg/ml against the isolated L. monocytogenes strain. At which not exhibit listeria growth up to 98.3 % and 96.1 %, respectively. H. A. coffee extract was screened using gas chromatography–mass spectrometry (GC-Mass). This analysis showed that the antibacterial impact of H. A. coffee extract against L. monocytogenes is attributed to the active components (caffeine and eugenol).
{"title":"Evaluation the antibacterial effect of hydroalcoholic coffee extract on L. monocytogenes isolated from milk and milk products","authors":"Basma J. Ahmed, L. El-Malt, Karima G. Abdel Hameed, Mona A El-Zamkan","doi":"10.21608/svu.2022.131641.1190","DOIUrl":"https://doi.org/10.21608/svu.2022.131641.1190","url":null,"abstract":"Listeria spp . is a very resilient bacteria that can persist hostile dairy-processing environments and consequently, food safety challenges appear here with respect to control its growth. L. monocytogenes is the most important species that cause a great concern in both food processing and public health. Therefore, this study was performed to investigate the incidence of Listeria spp. in 450 samples of raw milk, pasteurized milk, flavored milk, dominate cheese, kareish cheese, small scale ice cream, large scale ice cream, small scale yoghurt, and large scale yoghurt (50 each) were collected from dairy farms, local dairy shops and street vendors in Qena city, Egypt. Listeria spp. were isolated from 27 (6%) out of the total samples examined. They were recovered from the tested market raw milk, kareish cheese, small scale ice cream and domiati cheese samples with percentages of 20, 16, 14% and 4%, respectively. However, Listeria spp. was absent in other analyzed samples. The most prevalent species was L. monocytogenes 10 (2.22%), followed by L. grayii 9 (2%), L. welshimeri 4 (0.89%) and L. innocua 3 (0.67%) with an overall 5.7% verified by PCR. Eight out of 10 L. monocytogenes isolates were confirmed by the presence of the 16S rRNA gene that implies a risk of food-borne listeriosis among dairy product consumers. Furthermore, a unique approach was used for the assessment of antibacterial activity of different concentrations of gentamicin as a reference antibiotic standard and hydroalcoholic coffee extract (H. A. coffee extract) (1, 3, 10 and 30 µg/ml) against local isolated L. monocytogenes strain in vitro using a microplate in ELISA reader is provided in the current work. The most Potent antimicrobial activity of gentamicin and H. A. coffee extract was obtained at 30 µg/ml against the isolated L. monocytogenes strain. At which not exhibit listeria growth up to 98.3 % and 96.1 %, respectively. H. A. coffee extract was screened using gas chromatography–mass spectrometry (GC-Mass). This analysis showed that the antibacterial impact of H. A. coffee extract against L. monocytogenes is attributed to the active components (caffeine and eugenol).","PeriodicalId":22190,"journal":{"name":"SVU-International Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41950953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The effect of bio-filler-reinforced chitosan coating with types of solvent on internal changes and outer Abstract Due to the environmental hazards raised by using synthetic polymers, attention has turned to biodegradable polymers such as chitosan. The unique efficiency of chitosan coatings is severely affected by using the types of solvent as well as the fillers. To evaluate the effect of solvent types and application of cellulose nanocrystals in chitosan-based coatings on internal quality and eggshell morphology, an experiment was conducted with 4 treatments including chitosan coatings dissolved in acetic and/or lactic acids, with and without cellulose nanocrystal filler during 35 days at 25 o C. Results demonstrated that the reinforcement of biopolymer coatings matrix with cellulose nanocrystal had significantly improved weight loss, Haugh unit, and yolk index in coated eggs. Also, chitosan coating dissolved in acetic acid had better efficiency than lactic acid, as a view of the Haugh unit on days 21-28. At the same time, microscopic images of outer surface eggshells had somewhat confirmed the interior changes. In general, chitosan dissolved in acetic acid cellulose nanocrystal-reinforced coating can be used as an egg biodegradable packaging.
{"title":"The effect of bio-filler-reinforced chitosan coating with types of solvent on internal changes and outer eggshell morphology","authors":"Honeyeh Hajighasem Sharbatdar, Dariush Khademi Shurmasti","doi":"10.21608/svu.2022.129538.1184","DOIUrl":"https://doi.org/10.21608/svu.2022.129538.1184","url":null,"abstract":"The effect of bio-filler-reinforced chitosan coating with types of solvent on internal changes and outer Abstract Due to the environmental hazards raised by using synthetic polymers, attention has turned to biodegradable polymers such as chitosan. The unique efficiency of chitosan coatings is severely affected by using the types of solvent as well as the fillers. To evaluate the effect of solvent types and application of cellulose nanocrystals in chitosan-based coatings on internal quality and eggshell morphology, an experiment was conducted with 4 treatments including chitosan coatings dissolved in acetic and/or lactic acids, with and without cellulose nanocrystal filler during 35 days at 25 o C. Results demonstrated that the reinforcement of biopolymer coatings matrix with cellulose nanocrystal had significantly improved weight loss, Haugh unit, and yolk index in coated eggs. Also, chitosan coating dissolved in acetic acid had better efficiency than lactic acid, as a view of the Haugh unit on days 21-28. At the same time, microscopic images of outer surface eggshells had somewhat confirmed the interior changes. In general, chitosan dissolved in acetic acid cellulose nanocrystal-reinforced coating can be used as an egg biodegradable packaging.","PeriodicalId":22190,"journal":{"name":"SVU-International Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43145781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-07DOI: 10.21608/svu.2022.116749.1171
G. ABDEL-RAHEEM, Naglaa Ibrahim, A. Sayed, O. Amen, E. Mickdam
The current work aimed to evaluate the effect of feeding organic acids (OA) on growth performance, carcass traits, meat quality, blood parameters and immune response of broilers fed with a low protein diet (LPD). A total number of 68 broiler chicks (one-day-old) were randomly distributed into 4 equal groups each of 17 chicks. The first group was fed the basal diet (100% NRC crude protein (CP)) free from OA and considered as a control (T1). The other three groups (T2, T3 and T4) were fed on diets with different protein levels (95% NRC, 90% NRC & 85% NRC, respectively) and supplemented with OA at a level of 0.45%. The results showed that body weight and gain during starter phase (days 1-21) were not significantly (P = 0.7, P = 0.13, respectively) influenced by the supplementation of OA. However, during finisher phase (days 22-42) body weight and gain were significantly decreased (P < 0.05) in OA groups (-18% and -24%, respectively). A significant (P < 0.05) reduction in abdominal fat content and the meat cholesterol, triglyceride, and fat mass of broiler breast and thigh meat, while protein content significantly (P < 0.05) increased in all OA supplemented groups. In conclusion, adding OA improved immune response through increased serum globulin, and an increase in bursa relative weight of broilers. Moreover, the addition of OA to broiler fed LPD has no effect on growth parameters and carcass traits but improves broiler immunity and produces healthy meat to consumers.
{"title":"Impact of dietary supplementation of organic acids on the growth performance and immunity in broilers fed low protein diets","authors":"G. ABDEL-RAHEEM, Naglaa Ibrahim, A. Sayed, O. Amen, E. Mickdam","doi":"10.21608/svu.2022.116749.1171","DOIUrl":"https://doi.org/10.21608/svu.2022.116749.1171","url":null,"abstract":"The current work aimed to evaluate the effect of feeding organic acids (OA) on growth performance, carcass traits, meat quality, blood parameters and immune response of broilers fed with a low protein diet (LPD). A total number of 68 broiler chicks (one-day-old) were randomly distributed into 4 equal groups each of 17 chicks. The first group was fed the basal diet (100% NRC crude protein (CP)) free from OA and considered as a control (T1). The other three groups (T2, T3 and T4) were fed on diets with different protein levels (95% NRC, 90% NRC & 85% NRC, respectively) and supplemented with OA at a level of 0.45%. The results showed that body weight and gain during starter phase (days 1-21) were not significantly (P = 0.7, P = 0.13, respectively) influenced by the supplementation of OA. However, during finisher phase (days 22-42) body weight and gain were significantly decreased (P < 0.05) in OA groups (-18% and -24%, respectively). A significant (P < 0.05) reduction in abdominal fat content and the meat cholesterol, triglyceride, and fat mass of broiler breast and thigh meat, while protein content significantly (P < 0.05) increased in all OA supplemented groups. In conclusion, adding OA improved immune response through increased serum globulin, and an increase in bursa relative weight of broilers. Moreover, the addition of OA to broiler fed LPD has no effect on growth parameters and carcass traits but improves broiler immunity and produces healthy meat to consumers.","PeriodicalId":22190,"journal":{"name":"SVU-International Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41701382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-17DOI: 10.21608/svu.2022.125628.1181
Marwa Abdul-Ghani, A. Naser
Toxicity Alpha-Lipoic Acid Abstract Pharmacological and toxicological challenges are among the most important studies in revealing the latent properties of drugs. The goal of this study was to evaluate the effects of alpha-lipoic acid on the central nervous system and chlorpyrifos toxicity in a chick’s model. One-day-old chicks were used, the pharmacological challenge was done through propofol anesthesia and calculate the onset, duration, and recovery time, and while chlorpyrifos was used in the toxicological challenge for the median lethal doses LD 50s were determined using the up-and-down method. Alpha-lipoic acid at 20, 40, and 80 mg/kg caused an inhibition effect on the central nervous system, represented by a significant increase in the anesthesia period when chicks were anesthetized by propofol at 20 mg/kg intraperitoneally. Alpha-lipoic acid caused a significant decrease in the recovery period compared to the control group. Alpha-lipoic acid at 20 and 40 mg/kg increased the value of the median lethal dose of chlorpyrifos by 31 and 62%, respectively. The use of alpha-lipoic acid as preanesthetic has benefits such as decreased recovery time, furthermore Alpha-lipoic acid has a depressant effect in the central nervous system. Alpha-lipoic acid revealed an antidotal effect against chlorpyrifos toxicity.
{"title":"DETERMINATION OF CENTRAL NERVOUS EFFECT AND ANTIDOTAL EFFECT AGAINST CHLORPYRIFOS TOXICITY OF ALPHA-LIPOIC ACID THROUGH PHARMACOLOGICAL AND TOXICOLOGICAL CHALLENGES","authors":"Marwa Abdul-Ghani, A. Naser","doi":"10.21608/svu.2022.125628.1181","DOIUrl":"https://doi.org/10.21608/svu.2022.125628.1181","url":null,"abstract":"Toxicity Alpha-Lipoic Acid Abstract Pharmacological and toxicological challenges are among the most important studies in revealing the latent properties of drugs. The goal of this study was to evaluate the effects of alpha-lipoic acid on the central nervous system and chlorpyrifos toxicity in a chick’s model. One-day-old chicks were used, the pharmacological challenge was done through propofol anesthesia and calculate the onset, duration, and recovery time, and while chlorpyrifos was used in the toxicological challenge for the median lethal doses LD 50s were determined using the up-and-down method. Alpha-lipoic acid at 20, 40, and 80 mg/kg caused an inhibition effect on the central nervous system, represented by a significant increase in the anesthesia period when chicks were anesthetized by propofol at 20 mg/kg intraperitoneally. Alpha-lipoic acid caused a significant decrease in the recovery period compared to the control group. Alpha-lipoic acid at 20 and 40 mg/kg increased the value of the median lethal dose of chlorpyrifos by 31 and 62%, respectively. The use of alpha-lipoic acid as preanesthetic has benefits such as decreased recovery time, furthermore Alpha-lipoic acid has a depressant effect in the central nervous system. Alpha-lipoic acid revealed an antidotal effect against chlorpyrifos toxicity.","PeriodicalId":22190,"journal":{"name":"SVU-International Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47936621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-08DOI: 10.21608/svu.2022.119853.1176
Hager M. Beriry, Khalil Atef, Amany Sayed Gaber, Mouchira M Mohi ElDin
Ameliorative Effect of Extracts Induced Toxicity in in Rats. Abstract Parabens are group of preservatives chemical compounds used in cosmetics, personal hygiene product, food products and pharmaceuticals . In this study the hepatorenal toxicity due to exposure to paraben was evaluated. In this study design Twenty-four of albino rats experimentally used. Butyle paraben (BP) orally was given to animals 180 days in dose of 4.6 mgkg.bw which equal to10 % of the LD50 of BP. The extract of Mushroom was given by stomach tube in dose of 10 mg/kg/day for six 180 days. Exposure to Parabens exhibited histopathological and biochemical alterations. In the line of biochemical analysis BP induced toxic changes in both liver and kidney, the hepatic cellular enzymes concentrations (AST, ALT), Bilirubin, urea and creatinine were raised. In contrast plasma proteins were decreased in comparison to the control group. In renal and hepatic tissues BP administration induced vascular congestion as well as necrosis in the hepatorenal epithelium. Both the biochemical and histopathological alteration was improved after giving mushroom extracts in comparison to BP given group. In conclusion, mushroom extracts exhibited biochemical and histopathological improvement in liver and kidney against the toxicity by Butylparaben.
{"title":"Ameliorative Effect of Mushroom Extracts against Butyl Paraben Induced Toxicity in Liver and Kidney in Female Albino Rats.","authors":"Hager M. Beriry, Khalil Atef, Amany Sayed Gaber, Mouchira M Mohi ElDin","doi":"10.21608/svu.2022.119853.1176","DOIUrl":"https://doi.org/10.21608/svu.2022.119853.1176","url":null,"abstract":"Ameliorative Effect of Extracts Induced Toxicity in in Rats. Abstract Parabens are group of preservatives chemical compounds used in cosmetics, personal hygiene product, food products and pharmaceuticals . In this study the hepatorenal toxicity due to exposure to paraben was evaluated. In this study design Twenty-four of albino rats experimentally used. Butyle paraben (BP) orally was given to animals 180 days in dose of 4.6 mgkg.bw which equal to10 % of the LD50 of BP. The extract of Mushroom was given by stomach tube in dose of 10 mg/kg/day for six 180 days. Exposure to Parabens exhibited histopathological and biochemical alterations. In the line of biochemical analysis BP induced toxic changes in both liver and kidney, the hepatic cellular enzymes concentrations (AST, ALT), Bilirubin, urea and creatinine were raised. In contrast plasma proteins were decreased in comparison to the control group. In renal and hepatic tissues BP administration induced vascular congestion as well as necrosis in the hepatorenal epithelium. Both the biochemical and histopathological alteration was improved after giving mushroom extracts in comparison to BP given group. In conclusion, mushroom extracts exhibited biochemical and histopathological improvement in liver and kidney against the toxicity by Butylparaben.","PeriodicalId":22190,"journal":{"name":"SVU-International Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48010329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-03DOI: 10.21608/svu.2022.111977.1165
M. Zayed, Nasra A. Yousef, Ahmed S A Hassaneen
The aim of the present study was to evaluate the efficacy of pudendal nerve block as an alternative procedure to epidural analgesia for penile relaxation in male goats. Five healthy male goats were subjected to pudendal nerve block and lumbosacral epidural analgesia with a two-weeks interval as a washing period. Bilateral pudendal nerve block was performed using 1% of lidocaine hydrochloride (HCl) by ischiorectal fossa approach. Lumbosacral epidural analgesia was performed using 1% of lidocaine HCl in the lumbosacral space. Nociception was investigated by pinprick. Time to the onset and duration of the anti-nociception effect in the perineal region were recorded. Area of desensitization and penile relaxation were observed. Incoordination, ataxia, and analgesic effects were carefully observed and recorded according to the scoring system before analgesic administration and at 5-, 15-, 30-, 60-, and 90-min after administration. Pulse rate, respiratory rate, and rectal temperature were recorded at 0-, 5-, 15-, 30-, 60-, and 90-min after administration. This study showed that both the pudendal nerve block and the epidural analgesia resulted in analgesic effects, however, the penile relaxation without manipulation was observed in 4 out of 5 male goats subjected to the pudendal nerve block. None of the male goats subjected to lumbosacral epidural analgesia showed penile relaxation, spontaneously. The time of onset was significantly earlier in the pudendal nerve block compared to the epidural analgesia, Moreover, the duration of action was significantly shorter in the pudendal nerve block compared to the epidural analgesia. Incoordination, ataxia, and recumbency were observed in 3 out of 5 male goats subjected to epidural analgesia. In conclusion, the pudendal nerve block is likely superior to the epidural analgesia for examination of the penis and surgical procedures on the male goat reproductive system in the standing position.
{"title":"Pudendal Nerve Blockade Is Superior to Epidural Analgesia for Andrological and Surgical Procedures on the External Genitalia of Male Goats in the Standing Position","authors":"M. Zayed, Nasra A. Yousef, Ahmed S A Hassaneen","doi":"10.21608/svu.2022.111977.1165","DOIUrl":"https://doi.org/10.21608/svu.2022.111977.1165","url":null,"abstract":"The aim of the present study was to evaluate the efficacy of pudendal nerve block as an alternative procedure to epidural analgesia for penile relaxation in male goats. Five healthy male goats were subjected to pudendal nerve block and lumbosacral epidural analgesia with a two-weeks interval as a washing period. Bilateral pudendal nerve block was performed using 1% of lidocaine hydrochloride (HCl) by ischiorectal fossa approach. Lumbosacral epidural analgesia was performed using 1% of lidocaine HCl in the lumbosacral space. Nociception was investigated by pinprick. Time to the onset and duration of the anti-nociception effect in the perineal region were recorded. Area of desensitization and penile relaxation were observed. Incoordination, ataxia, and analgesic effects were carefully observed and recorded according to the scoring system before analgesic administration and at 5-, 15-, 30-, 60-, and 90-min after administration. Pulse rate, respiratory rate, and rectal temperature were recorded at 0-, 5-, 15-, 30-, 60-, and 90-min after administration. This study showed that both the pudendal nerve block and the epidural analgesia resulted in analgesic effects, however, the penile relaxation without manipulation was observed in 4 out of 5 male goats subjected to the pudendal nerve block. None of the male goats subjected to lumbosacral epidural analgesia showed penile relaxation, spontaneously. The time of onset was significantly earlier in the pudendal nerve block compared to the epidural analgesia, Moreover, the duration of action was significantly shorter in the pudendal nerve block compared to the epidural analgesia. Incoordination, ataxia, and recumbency were observed in 3 out of 5 male goats subjected to epidural analgesia. In conclusion, the pudendal nerve block is likely superior to the epidural analgesia for examination of the penis and surgical procedures on the male goat reproductive system in the standing position.","PeriodicalId":22190,"journal":{"name":"SVU-International Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45259396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.21608/svu.2022.114720.1170
A. S. Mohamed, L. El-Malt, Karima G. Abdel Hameed, Mona A El-Zamkan
A total of 225 random samples of raw cow milk (150), Kareish cheese, Domiati cheese and butter (25 samples each), were collected from various farmers’ houses, dairy shops, street vendors and supermarkets located at Aswan city, Egypt, to be examined by culture and molecular methods in which 16S rRNA gene was used; the obtained PCR product was then purified and sequenced to detect the existence of Chryseobacterium spp. All cheese and butter samples didn’t show the characteristic colonies of Chryseobacterium spp., while two raw cow milk samples exhibited the characteristic Chryseobacterium colonies. The suspected Chryseobacterium spp. were confirmed by PCR and DNA sequencing and comparing the sequenced DNA with those on GenBank using the BLAST tool on GenBank, underlining its occurrence in raw cow milk samples with an incidence of 1.3%. The isolated strains showed the highest similarity with Chryseobacterium jip 108/83 (100%). According to obtained data and our knowledge, it is the first time to isolate Chryseobacterium spp. from raw milk in Egypt. The isolated strains were registered on the GenBank with the proposed names Chryseobacterium Egyam strain AM1 (Accession No. MK059947) and Chryseobacterium Egyam strain AM2 (Accession No. MK059948). A constructed phylogenetic tree by using the neighbour-joining method was applied to detect the exact taxonomic position of the obtained strains.
{"title":"Incidence of Chryseobacterium spp. in raw milk and some dairy products","authors":"A. S. Mohamed, L. El-Malt, Karima G. Abdel Hameed, Mona A El-Zamkan","doi":"10.21608/svu.2022.114720.1170","DOIUrl":"https://doi.org/10.21608/svu.2022.114720.1170","url":null,"abstract":"A total of 225 random samples of raw cow milk (150), Kareish cheese, Domiati cheese and butter (25 samples each), were collected from various farmers’ houses, dairy shops, street vendors and supermarkets located at Aswan city, Egypt, to be examined by culture and molecular methods in which 16S rRNA gene was used; the obtained PCR product was then purified and sequenced to detect the existence of Chryseobacterium spp. All cheese and butter samples didn’t show the characteristic colonies of Chryseobacterium spp., while two raw cow milk samples exhibited the characteristic Chryseobacterium colonies. The suspected Chryseobacterium spp. were confirmed by PCR and DNA sequencing and comparing the sequenced DNA with those on GenBank using the BLAST tool on GenBank, underlining its occurrence in raw cow milk samples with an incidence of 1.3%. The isolated strains showed the highest similarity with Chryseobacterium jip 108/83 (100%). According to obtained data and our knowledge, it is the first time to isolate Chryseobacterium spp. from raw milk in Egypt. The isolated strains were registered on the GenBank with the proposed names Chryseobacterium Egyam strain AM1 (Accession No. MK059947) and Chryseobacterium Egyam strain AM2 (Accession No. MK059948). A constructed phylogenetic tree by using the neighbour-joining method was applied to detect the exact taxonomic position of the obtained strains.","PeriodicalId":22190,"journal":{"name":"SVU-International Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47268540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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