Pub Date : 2022-04-01DOI: 10.21608/svu.2022.117123.1172
Faten Fareed, Mona A El-Zamkan, Ahmed Shaban Ahmed, Aml Mokhtar, Enas A. Noseer, M. Salah, A. Nassar, Rofida F. Moftah, I. Sabeq, Obeid Shanab
Cheeses are among high-protein containing foodstuffs in which enzymatic and microbial activities cause the formation of the biogenic amines from amino acid decarboxylation. A total of 80 samples of old Kareish cheese and ripened Domiati cheese (40 samples each) were collected from local dairy shops and vendors and dairy cattle rural house in Qena city, Egypt. The concentration of biogenic amines (Tyramine, Histamine, Cadaverine and Putrescine) were assayed via using high performance liquid chromatography (HPLC). In old Kareish cheese samples, histamine had the highest concentration followed by tyramine, cadaverine and putrescine with means of 835±32.9, 564±37.1, 556±27.4 and 537±37.5, respectively. Also, histamine (513±29.1) was the highest among the detected biogenic amines in ripened Domiati cheese followed by putrescine (492±32.2), tyramine (478×±25.3) and cadaverine (470±30). In conclusion our data showed higher levels of tyramine, histamine, putrescine and cadaverine in old Kareish and ripened Domiati cheese. The highest levels were detected in old Kareish cheese in compare with ripened Domiati cheese.
{"title":"Biogenic Amines Levels in Old Kariesh Cheese and Ripened Domiati Cheese, Qena, Egypt","authors":"Faten Fareed, Mona A El-Zamkan, Ahmed Shaban Ahmed, Aml Mokhtar, Enas A. Noseer, M. Salah, A. Nassar, Rofida F. Moftah, I. Sabeq, Obeid Shanab","doi":"10.21608/svu.2022.117123.1172","DOIUrl":"https://doi.org/10.21608/svu.2022.117123.1172","url":null,"abstract":"Cheeses are among high-protein containing foodstuffs in which enzymatic and microbial activities cause the formation of the biogenic amines from amino acid decarboxylation. A total of 80 samples of old Kareish cheese and ripened Domiati cheese (40 samples each) were collected from local dairy shops and vendors and dairy cattle rural house in Qena city, Egypt. The concentration of biogenic amines (Tyramine, Histamine, Cadaverine and Putrescine) were assayed via using high performance liquid chromatography (HPLC). In old Kareish cheese samples, histamine had the highest concentration followed by tyramine, cadaverine and putrescine with means of 835±32.9, 564±37.1, 556±27.4 and 537±37.5, respectively. Also, histamine (513±29.1) was the highest among the detected biogenic amines in ripened Domiati cheese followed by putrescine (492±32.2), tyramine (478×±25.3) and cadaverine (470±30). In conclusion our data showed higher levels of tyramine, histamine, putrescine and cadaverine in old Kareish and ripened Domiati cheese. The highest levels were detected in old Kareish cheese in compare with ripened Domiati cheese.","PeriodicalId":22190,"journal":{"name":"SVU-International Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46403738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.21608/svu.2022.133515.1192
L. Taha, Zainab M. Maher, Ahmed E. Nassar, Mohamed-Cherif Abdallah, H. Embark, M. Youssef, A. Abdeen, Rofida F. Moftah, H. Madhyastha, Obeid Shanab
source Abstract Nitrosodiethylamine (NDEA) is a potent oxidant induces neurodegeneration via (reactive oxygen species) ROS. Copper is an important metal essential for scavenging free radicals, development of central nervous system (CNS) and redox angiogenesis signaling. Vascular endothelial growth factor (VEGF) is well known as efficacious and long-term signal that stimulates angiogenesis, where its expression is copper dependent. We examined the copper protective effect against brain vascular damage initiated by NDEA. NDEA induces brain vascular wall damage, necrosis with interstitial hemorrhage and diminishes VEGF expression. Histopathological examination showing a great improvement of brain tissue in copper treated mice with significant increase in VEGF expression. Higher levels of intracellular copper can stimulate angiogenesis and exhibited a significant protection against NDEA induced brain vascular damage, confirming its ability to enhance antioxidant activity and angiogenesis initiation. Our report presents first evidence that inducible VEGF expression in brain is sensitive to copper; moreover, copper-based therapeutics represents a novel approach to reduce brain vascular damage induced by NDEA generated ROS.
{"title":"Molecular mechanism of Copper Albumin Complex on NDEA induced brain vascular damage via promoting VEGF expression.","authors":"L. Taha, Zainab M. Maher, Ahmed E. Nassar, Mohamed-Cherif Abdallah, H. Embark, M. Youssef, A. Abdeen, Rofida F. Moftah, H. Madhyastha, Obeid Shanab","doi":"10.21608/svu.2022.133515.1192","DOIUrl":"https://doi.org/10.21608/svu.2022.133515.1192","url":null,"abstract":"source Abstract Nitrosodiethylamine (NDEA) is a potent oxidant induces neurodegeneration via (reactive oxygen species) ROS. Copper is an important metal essential for scavenging free radicals, development of central nervous system (CNS) and redox angiogenesis signaling. Vascular endothelial growth factor (VEGF) is well known as efficacious and long-term signal that stimulates angiogenesis, where its expression is copper dependent. We examined the copper protective effect against brain vascular damage initiated by NDEA. NDEA induces brain vascular wall damage, necrosis with interstitial hemorrhage and diminishes VEGF expression. Histopathological examination showing a great improvement of brain tissue in copper treated mice with significant increase in VEGF expression. Higher levels of intracellular copper can stimulate angiogenesis and exhibited a significant protection against NDEA induced brain vascular damage, confirming its ability to enhance antioxidant activity and angiogenesis initiation. Our report presents first evidence that inducible VEGF expression in brain is sensitive to copper; moreover, copper-based therapeutics represents a novel approach to reduce brain vascular damage induced by NDEA generated ROS.","PeriodicalId":22190,"journal":{"name":"SVU-International Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42763683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.21608/svu.2022.137345.1199
T. Tawfik, H. Gad-Elrab, N. Abdel-Aziz
Competing Abstract A total of 180 samples of fish meat and canned fish were randomly collected from different markets and retail shops in Sohag city as the following; fresh water fish (Nile tilapia and catfish), marine water fish (mullet), shrimps, and canned fish (tuna and salmon) with 30 samples of each, to study the incidence of Aeromonas species (Aeromonas spp.) with special reference to Aeromonas hydrophila ( A.hydrophila ) and its virulence genes. The results of this study showed that the mean of Aeromonas counts were 0.122×10 2 , 0.504×10 2 , 0.124×10 2 , 0.037×10 2 cfu/g for Nile tilapia, catfish, mullet and shrimps, respectively. While in canned fish it was uncountable . Aeromonas spp. were isolated from 60 of 180 examined samples with a percentage of 33.3%, 7 species were identified: A. caviae, A. hydrophila , A. media, A. shubertii , A. sobria, A. veronii biovar sobria and A. veronii biovar veronii were detected at a percentage of 5%, 7.8%, 2.8%, 7.2%, 5%, 2.8% and 2.8%, respectively. The results of PCR showed that, 12 isolates out of 14 were positive for 16S rRNA gene of A. hydrophila with a percentage of 85.7 %. Virulence gene like, Aerolysin AHA was found in 41.6 % of the examined samples while, the heat stable enterotoxin AST gene was not detected. This study spots the lights on Aeromonas spp. especially A. hydrophila as potential biological hazard in fish meat and canned fish, as a foodborne pathogen.
{"title":"Incidence of Aeromonas species isolated from fresh fish , canned fish and shrimp in Sohag Governorate, Egypt","authors":"T. Tawfik, H. Gad-Elrab, N. Abdel-Aziz","doi":"10.21608/svu.2022.137345.1199","DOIUrl":"https://doi.org/10.21608/svu.2022.137345.1199","url":null,"abstract":"Competing Abstract A total of 180 samples of fish meat and canned fish were randomly collected from different markets and retail shops in Sohag city as the following; fresh water fish (Nile tilapia and catfish), marine water fish (mullet), shrimps, and canned fish (tuna and salmon) with 30 samples of each, to study the incidence of Aeromonas species (Aeromonas spp.) with special reference to Aeromonas hydrophila ( A.hydrophila ) and its virulence genes. The results of this study showed that the mean of Aeromonas counts were 0.122×10 2 , 0.504×10 2 , 0.124×10 2 , 0.037×10 2 cfu/g for Nile tilapia, catfish, mullet and shrimps, respectively. While in canned fish it was uncountable . Aeromonas spp. were isolated from 60 of 180 examined samples with a percentage of 33.3%, 7 species were identified: A. caviae, A. hydrophila , A. media, A. shubertii , A. sobria, A. veronii biovar sobria and A. veronii biovar veronii were detected at a percentage of 5%, 7.8%, 2.8%, 7.2%, 5%, 2.8% and 2.8%, respectively. The results of PCR showed that, 12 isolates out of 14 were positive for 16S rRNA gene of A. hydrophila with a percentage of 85.7 %. Virulence gene like, Aerolysin AHA was found in 41.6 % of the examined samples while, the heat stable enterotoxin AST gene was not detected. This study spots the lights on Aeromonas spp. especially A. hydrophila as potential biological hazard in fish meat and canned fish, as a foodborne pathogen.","PeriodicalId":22190,"journal":{"name":"SVU-International Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42211963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.21608/svu.2022.131346.1187
Samah M. Alaa-Eldein, Mohammed Abdelsabour-Khalaf, E. A. Hafez, S. Soliman
Competing Abstract This study was evaluating histopathological changes in lung tissue induced by formaldehyde vapors in mice. A group of 20 adult male mice was divided into two groups for this purpose. The mice in group (I) are the controls, whereas the mice in groups (II) were exposed to formaldehyde vapors inhalation in a toxic dose (0.5 PPM) every day for two months in the students dissecting room of South Valley University's Faculty of Veterinary Medicine. Lung samples were prepared for light microscopic examination after exposure. Gross structural abnormalities in the lungs were identified, including congestion in most lobes and localized pneumonic organization. Thickening of the alveolar septum, epithelial hyperplasia in the bronchioles, proliferative capillaries, pulmonary vasculitis, and hyperplastic lymphocytic aggregations in the parabronchiolar area, pulmonary fibrosis, and precancerous modifications were detected under light microscopy (metaplasia of goblet cells and dysplasia of the bronchiolar epithelium). It was found that inhaling formaldehyde has an irritating toxic effect in addition to its harmful carcinogenic effect on the lungs of mice, which is related to exposure durations.
{"title":"Effect of exposure to formalin on the histochemical structure of respiratory organs in Mice","authors":"Samah M. Alaa-Eldein, Mohammed Abdelsabour-Khalaf, E. A. Hafez, S. Soliman","doi":"10.21608/svu.2022.131346.1187","DOIUrl":"https://doi.org/10.21608/svu.2022.131346.1187","url":null,"abstract":"Competing Abstract This study was evaluating histopathological changes in lung tissue induced by formaldehyde vapors in mice. A group of 20 adult male mice was divided into two groups for this purpose. The mice in group (I) are the controls, whereas the mice in groups (II) were exposed to formaldehyde vapors inhalation in a toxic dose (0.5 PPM) every day for two months in the students dissecting room of South Valley University's Faculty of Veterinary Medicine. Lung samples were prepared for light microscopic examination after exposure. Gross structural abnormalities in the lungs were identified, including congestion in most lobes and localized pneumonic organization. Thickening of the alveolar septum, epithelial hyperplasia in the bronchioles, proliferative capillaries, pulmonary vasculitis, and hyperplastic lymphocytic aggregations in the parabronchiolar area, pulmonary fibrosis, and precancerous modifications were detected under light microscopy (metaplasia of goblet cells and dysplasia of the bronchiolar epithelium). It was found that inhaling formaldehyde has an irritating toxic effect in addition to its harmful carcinogenic effect on the lungs of mice, which is related to exposure durations.","PeriodicalId":22190,"journal":{"name":"SVU-International Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44016513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-08DOI: 10.21608/svu.2022.105577.1163
Sotohy A. Mohamed, Ehsan A. Hassan, A. Ezzat, Ahmed E. Sadek
{"title":"Prevalence and Antibiogram profiles of STEC strains isolated from raw cow's milk in New-Valley Governorate, Egypt","authors":"Sotohy A. Mohamed, Ehsan A. Hassan, A. Ezzat, Ahmed E. Sadek","doi":"10.21608/svu.2022.105577.1163","DOIUrl":"https://doi.org/10.21608/svu.2022.105577.1163","url":null,"abstract":"","PeriodicalId":22190,"journal":{"name":"SVU-International Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41925727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21608/svu.2022.88338.1136
M. Mohamed, A. E. A. Mohamed
Competing interest: The authors have declared that no competing interest exists. Abstract The present study investigated the morphometric measurements in 44 red foxes in two developmental stages young and adult of different sexes male and female, from three localities of Egypt Giza, Sohag, and Qena governorates, Egypt, during the period from February 2016 to April 2021 along all year`s period except during winter to avoid stress and potential injury to pups and adults. Foxes were immobilized using injected intramuscularly in femoral or scapular body areas before the examination. Different measurements were selected; head and body length (HBL), tail length (TaL), hindfoot length (HFL), and neck circumference (NC) besides body weight. Results revealed that bodyweight, head and body length, and tail length in adult male foxes were significantly higher than in adult females. The results also revealed that all morphometric measurements besides body weight were significantly higher in adult male animals than young males, also the same parameters were higher in adult female animals than young females.
{"title":"Morphometric measurements of red foxes (Vulpes Vulpes) in Egypt","authors":"M. Mohamed, A. E. A. Mohamed","doi":"10.21608/svu.2022.88338.1136","DOIUrl":"https://doi.org/10.21608/svu.2022.88338.1136","url":null,"abstract":"Competing interest: The authors have declared that no competing interest exists. Abstract The present study investigated the morphometric measurements in 44 red foxes in two developmental stages young and adult of different sexes male and female, from three localities of Egypt Giza, Sohag, and Qena governorates, Egypt, during the period from February 2016 to April 2021 along all year`s period except during winter to avoid stress and potential injury to pups and adults. Foxes were immobilized using injected intramuscularly in femoral or scapular body areas before the examination. Different measurements were selected; head and body length (HBL), tail length (TaL), hindfoot length (HFL), and neck circumference (NC) besides body weight. Results revealed that bodyweight, head and body length, and tail length in adult male foxes were significantly higher than in adult females. The results also revealed that all morphometric measurements besides body weight were significantly higher in adult male animals than young males, also the same parameters were higher in adult female animals than young females.","PeriodicalId":22190,"journal":{"name":"SVU-International Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45857276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21608/svu.2022.90304.1140
A. Ahmed, Adel elasyed ahmed, Abu El-Magd M. Mohamed, A. Ali
Competing Abstract Bovine theileriosis, babesiosis and anaplasmosis are tick-borne hemoparasitic diseases and they are responsible for huge economic losses in livestock sector in Egypt. Currently, a total number of 110 dairy cows from different regions of Qena governorate, Egypt, were clinically and laboratory investigated for diagnosis of theileriosis, babesiosis and anaplasmosis using Giemsa stained blood film and Polymerase Chain Reaction (PCR) assay during the period from January 2019 to December 2019. On the basis of the obtained results, the overall prevalence of theileriosis, babesiosis and anaplasmosis among the screened cattle was 21.81%, 9.09% and 25.45 respectively. Furthermore, mixed infections were seen in nineteen cases (17.27%) on the basis of blood film examination. PCR assay results revealed that, the infection rate with theileriosis, babesiosis and anaplasmosis was 5.12%, 10.25% and 35.89%, respectively. While, 3 cows (7.69%) were found to harbor a mixed infection. Additionally, hemato-biochemical alternations in theileriosis, babesiosis and anaplasmosis infected cows were also detected in this study. It could be concluded that PCR assay was the most sensitive test in the detection of the infection in all cases of the disease (acute, chronic and carriers) as once animals infected, they become carriers with low parasitemia after recovery and this low parasitemia cannot be detected by traditional examination of Giemsa stained thin blood smears.
{"title":"Clinical and Laboratory Diagnosis of Some Blood Parasites in Dairy Cows in Qena Governorate","authors":"A. Ahmed, Adel elasyed ahmed, Abu El-Magd M. Mohamed, A. Ali","doi":"10.21608/svu.2022.90304.1140","DOIUrl":"https://doi.org/10.21608/svu.2022.90304.1140","url":null,"abstract":"Competing Abstract Bovine theileriosis, babesiosis and anaplasmosis are tick-borne hemoparasitic diseases and they are responsible for huge economic losses in livestock sector in Egypt. Currently, a total number of 110 dairy cows from different regions of Qena governorate, Egypt, were clinically and laboratory investigated for diagnosis of theileriosis, babesiosis and anaplasmosis using Giemsa stained blood film and Polymerase Chain Reaction (PCR) assay during the period from January 2019 to December 2019. On the basis of the obtained results, the overall prevalence of theileriosis, babesiosis and anaplasmosis among the screened cattle was 21.81%, 9.09% and 25.45 respectively. Furthermore, mixed infections were seen in nineteen cases (17.27%) on the basis of blood film examination. PCR assay results revealed that, the infection rate with theileriosis, babesiosis and anaplasmosis was 5.12%, 10.25% and 35.89%, respectively. While, 3 cows (7.69%) were found to harbor a mixed infection. Additionally, hemato-biochemical alternations in theileriosis, babesiosis and anaplasmosis infected cows were also detected in this study. It could be concluded that PCR assay was the most sensitive test in the detection of the infection in all cases of the disease (acute, chronic and carriers) as once animals infected, they become carriers with low parasitemia after recovery and this low parasitemia cannot be detected by traditional examination of Giemsa stained thin blood smears.","PeriodicalId":22190,"journal":{"name":"SVU-International Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42483114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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