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Gamma rays and EMS induced Flower Color Mutation in Grasspea (Lathyrus sativus Linn.) 伽玛射线和EMS诱导的草花颜色突变
4区 农林科学 Pub Date : 2017-09-16 DOI: 10.15832/ANKUTBD.385866
P. Ramezani, M. Siavoshi, A. More, M. Ebrahimi
To induce flower color mutants in Grasspea ( Lathyrus sativus Linn.) variety “Pusa-24“, the fresh seeds were exposed to 4 different doses of gamma radiation as physical mutagens (100, 200, 300 and 400 Gy) and treated with 4 different concentration of Ethyl Methanesulphonate as chemical mutagen (5, 10, 15 and 20 mM ) and also combination of both mutagens. Due to mutagenesis broad range of flower color mutants in M 2 , M 3 and M 4 were obtained. Various unique and different flower color mutations like pink, purple, white and yellow detected against blue which is natural color in control progenies. Maximum flower color frequency was recorded in EMS treated seeds (20 mM) in 4th generation and lowest mutation frequency in 2nd generation was obtained in gamma rays (100 Gy) treated seeds. The results showed more flower color mutations happened in more dose /concentration of mutagens in every generation
采用4种不同剂量的物理诱变剂(100、200、300和400 Gy)和4种不同浓度的化学诱变剂(5、10、15和20 mM),以及这两种诱变剂的组合,诱导草品种“Pusa-24”的花色突变体。通过诱变获得了广泛的花色突变体m2、m3和m4。在对照后代中检测到粉红色、紫色、白色和黄色等各种独特和不同的花色突变,而蓝色是自然色。第4代EMS处理种子的花色频率最高(20 mM),第2代γ射线处理种子的花色频率最低(100 Gy)。结果表明,每代诱变剂剂量/浓度越大,发生的花色突变越多
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引用次数: 3
Determination of Nuclear DNA Content and Ploidy of Hypericum perforatum L. Accessions Collected From Western Turkey 土耳其西部贯叶连翘种质核DNA含量及倍性测定
4区 农林科学 Pub Date : 2017-09-16 DOI: 10.15832/tbd.06745
G. S. Tuna, Gozde Duyu, Kardelen Uzun, G. Yücel, M. Tuna
Abstract Hypericum perforatum L. (St John’s Wort) is a medicinal plant that produces pharmaceutically important compounds with antidepressive, anticancer and antiviral activities. H. perforatum is a facultative apomictic species as it has the ability to reproduce with multiple reproduction mechanisms affecting genetic structure and chemical composition of the plants.  The objective of this study was to determine nuclear DNA content and ploidy of H. perforatum L. plants growing naturally in the flora of Turkey. The seeds of 39 Hypericum perforatum L. accessions collected from 23 different locations in Turkey were used in the study. Nuclear DNA content of three different seedlings for each of the 39 H. perforatum accessions was determined using flow cytometry. Based on the results of flow cytometric analysis, nuclear DNA content of the accessions varied between 0.8 - 2.57 pg2C -1 . Nuclear DNA content differences observed among H. perforatum accessions were statistically significant (P< 0.01) and the Duncan test revealed that the accessions formed three clearly distinguishable main groups. Mean nuclear DNA contents of each group were 0.80 pg2C -1 , 1.58 pg2C -1 (1.36-1.73 pg2C -1 ) and 2.38 pg2C -1 (2.15-2.57 pg2C -1 ). Ploidy levels were determined by correlating nuclear DNA content of accessions with the number of their mitotic chromosomes by counting chromosome number of one plant from each group. Findings suggest that H. perforatum accessions used in this study had the following three different ploidy levels: diploid, tetraploid and hexaploid.  The percentage of diploid, tetraploid and hexaploid accessions was 2.2, 86.4 and 11.4% respectively.
贯叶连翘(Hypericum perforatum L., St John’s Wort)是一种具有抗抑郁、抗癌和抗病毒活性的重要药用植物。贯叶连翘是兼性无融合生殖植物,具有多种繁殖机制,影响植物的遗传结构和化学成分。本研究的目的是确定在土耳其植物区系中自然生长的贯叶连翘(H. perforatum L.)的核DNA含量和倍性。以土耳其23个不同地点收集的39份贯叶连翘种子为研究材料。采用流式细胞术测定了39株贯叶连翘3个不同幼苗的核DNA含量。流式细胞分析结果显示,各种质核DNA含量在0.8 ~ 2.57 pg2C -1之间。各种质间核DNA含量差异有统计学意义(P< 0.01), Duncan检验表明,各种质间形成了3个明显可区分的主要类群。各组平均核DNA含量分别为0.80、1.58(1.36 ~ 1.73)、2.38(2.15 ~ 2.57)。通过计算每组一株植物的染色体数目,将核DNA含量与其有丝分裂染色体数目的相关性来确定倍性水平。结果表明,本研究使用的贯叶连翘材料具有三种不同的倍性水平:二倍体、四倍体和六倍体。二倍体、四倍体和六倍体的比例分别为2.2%、86.4和11.4%。
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引用次数: 5
Gene Action and Useful Heterosis in Interspesific Cotton Crosses (Gossypium hirsutum L. x Gossypium barbadense L.) 棉种间杂交(棉棉)的基因作用及有益杂种优势
4区 农林科学 Pub Date : 2017-09-16 DOI: 10.15832/ANKUTBD.385864
M. Çoban, Aydın Ünay
Conducted the breeding researches with interspecific cotton crosses are aimed to obtain genotypes have desired fiber characteristics like Gossypium barbadense L. and have high yields potential like Gossypium hirsutum L.. In this study, gene action and useful heterosis were investigated for fiber quality and yield traits in interspecific cotton crosses. The 12 F 1 ’s and their parents were evaluated in a Randomized Complete Block Design with four replications at the Nazilli Cotton Research Institute’s fields during 2011 and 2012 cotton growing season. Claudia, Candia, Sahin 2000, BA 308, Naz 07 and Fantom ( Gossypium hirsutum L.) were used as a female parents and Giza 45 and Avesto ( Gossypium barbadense L.) used as a male parents. Non-additive gene effects for yields, fiber strength and fiber length were greater than additive gene effects. The useful heterosis were positively significant for fiber strength and fiber length. Candia x Giza 45 hybrid was promising hybrid for all observed characters.
通过棉花种间杂交育种研究,获得具有巴氏棉(Gossypium barbadense L.)等优良纤维特性和具有毛棉(Gossypium hirsutum L.)等高产潜力的基因型。本研究对棉花种间杂交的纤维品质和产量性状进行了基因作用和有用杂种优势的研究。采用4个重复的随机完全区组设计,于2011年和2012年棉花生长季在Nazilli棉花研究所的田间对12个f1及其亲本进行评价。以Claudia、Candia、Sahin 2000、BA 308、Naz 07和Fantom(棉棉)为雌性亲本,以Giza 45和Avesto(棉棉)为雄性亲本。产量、纤维强度和纤维长度的非加性基因效应大于加性基因效应。有用杂种优势在纤维强度和纤维长度上呈显著正相关。在所有观察到的性状中,Candia x Giza 45都是有前途的杂交种。
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引用次数: 3
Borçka Barajı Rezervuarında Yeni Oluşan Sediment Birikim Sahalarında Penetrasyon Direnç Değerlerinin Yersel Dağılımı
4区 农林科学 Pub Date : 2017-09-06 DOI: 10.15832/TBD.14557
Bülent Turgut, Mehmet Özalp, Taşkın Öztaş
Bu calisma, Borcka Baraji rezervuar sahasinda yeni biriken sedimentlerin derinlige bagli olarak penetrasyon direnc degerlerindeki degisimini ve incelenen bu ozelligin birikim katmanlarindaki yersel bagimlilik derecesini belirlemek amaciyla yurutulmustur. Calisma alaninda olusturulan gridler yardimiyla 91 noktada dijital penetrometre kullanilarak 80 cm derinlik boyunca penetrasyon direnci (PD) olculmustur. Calisma sonucunda ortalama PD’nin tum olcum noktalarinda 1MPa’in altinda oldugu, yuzey katmaninda (0-10cm) en yuksek degere (0.747MPa) ulastigi ve derinlige bagli olarak azaldigi belirlenmistir. Bununla beraber tanimlayici istatistik sonuclari (standart sapma: 0.123; degisim genisligi:0.62) ve dagilim haritalarina gore PD cevresel faktorlerden daha fazla etkilenen yuzey sediment katmaninda heterojen bir dagilim gostermistir. Ayrica yersel bagimlilik derecesi ve semivariogram model parametreleri bakimindan da birikim katmanlari arasinda farkliliklarin oldugu ortaya konulmustur.
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引用次数: 0
Selection of Suitable Sites for Small Ruminant Farming Using Remote Sensing and Geographic Information System 基于遥感和地理信息系统的小反刍动物适宜养殖地点选择
4区 农林科学 Pub Date : 2017-09-06 DOI: 10.15832/TBD.26236
Esin Deri, H. Ünal
The aim of this study was to determine the most suitable areas for small ruminant  farming in the Karaburun area in Izmir province, Turkey. To this purpose, an inquiry  model was first developed using remote sensing and a geographic information system.  In developing the model, legal and technical factors were taken into consideration, and  eight evaluation criteria (distance from settled areas, distance from lakes or similar  water sources, distance from protected water catchment basins, distance from wind energy generators, distance from irrigation and drainage canals, slope, aspect – direction  of slope – and land use class) and three evaluation classes in relation to these criteria  (suitable, conditionally suitable and unsuitable) were planned. Later, the model was  used to test the suitability of the study area in general and five sample farms in that area  for suitability. According to all of the criteria of evaluation, 3.54% of the 42 707.15 ha 15 study area was found to be suitable for small ruminant farming, 2.78% was  conditionally suitable, and 93.60% was unsuitable. As for the five sample farms in the  study area, none of them was found to be suitable according to all of the evaluation  criteria. In addition, suggestions were made for the functionality and effectiveness in  use of the geography information inquiry models used in the choice of places for small ruminant farming.
本研究的目的是确定土耳其伊兹密尔省卡拉布伦地区最适合小型反刍动物养殖的地区。为此目的,首先利用遥感和地理信息系统开发了一种查询模式。在开发模型时,考虑了法律和技术因素,并考虑了八个评估标准(距离定居地区的距离,距离湖泊或类似水源的距离,距离受保护的集水区的距离,距离风力发电机的距离,距离灌溉和排水渠的距离,坡度,坡向-方向-和土地利用类别)和与这些标准相关的三个评估类别(适合,条件适宜和不适宜)计划。随后,利用该模型对研究区总体及5个样本养殖场的适宜性进行了测试。综合各项评价指标,42 707.15 ha研究区适宜小反刍动物养殖的面积为3.54%,有条件适宜的面积为2.78%,不适宜的面积为93.60%。研究区5个样本养殖场均不符合所有评价标准。此外,对地理信息查询模型在小反刍动物养殖场所选择中的功能和有效性提出了建议。
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引用次数: 0
Laboratuar Koşullarında Otomatik Bir Patates Dikim Makinasının Performansı Üzerine Kepçe Büyüklüğünün, Tohum Karakteristiklerinin ve Açısal Hızın Etkisi
4区 农林科学 Pub Date : 2017-09-06 DOI: 10.15832/TBD.99408
M. Boydaş
Tohum dagitma duzeni patates dikim makinalarinin en temel kismini olusturmaktadir. Sira uzeri tohum dagilim duzgunlugundeki dogruluk, tohum dagitma duzeninin kabiliyetine baglidir. Tohum dagitma duzeninin bu kabiliyeti yapisal ve calisma sartlarina direkt olarak baglidir. Bu calisma, tam otomatik bir patates dikim makinasinda farkli buyuklukte kepcelerin (C1
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引用次数: 0
Coğrafi Bilgi Sistemi, Uzaktan Algılama ve İstatistik Yaklaşımlar Kullanarak Çok Kriterli Değerlendirme İle Erozyon Risk Belirlenmesi. Örnek Çalışma; Ankara-Güvenç Havzası
4区 农林科学 Pub Date : 2017-09-06 DOI: 10.15832/tbd.07240
İnci Turan, Orhan Dengi̇z
Ozet Bu calismanin amaci, Ankara Guvenc Havzasi’nin cografi bilgi sistemi (CBS), uzaktan algilama (UA) ve istatistik yaklasimlar kullanarak cok kriterli degerlendirme ile erozyon risk sinifinin belirlenmesi ve haritalanmasidir. Calisma havzasi yaklasik 17.3 km² lik bir alani kapsamaktadir. Erozyon risk siniflarinin belirlenmesi amaciyla Havza’da toprak erozyonunun meydana gelmesinde yedi adet parametre (bunye, toprak derinligi, yagis, arazi kullanimi, yukseklik, egim ve bitki ortusu) ve bu parametrelere ait alt kriterler ele alinmistir. Parametrelere ait veriler icin calisma alaninin 1:25.000 olcekli sayisal temel toprak haritasi ve topografik harita ile arazi kullanimi-arazi ortusunun ve bitki ortusunun belirlenmesi amaciyla Landsat 7 uydu goruntusu kullanilmistir. Parametrelerin onceliklerinin belirlenmesinde Analitik Hiyerarsik Surec (AHS) teknigi, alt kriterlerin onceliklerinin belirlenmesinde ise Z-Skor istatistik analizi tekniginden yararlanilmistir. Nihai olarak ise dort siniftan olusan erozyon duyarlilik siniflarinin belirlenmesinde de dogrusal kombinasyon teknigi kullanilmistir. Elde edilen analiz sonuclarina gore havzanin  % 45’i cok siddetli erozyon riskini tasidigi belirlenmistir. Anahtar Kelimeler: Erozyon risk belirleme; Z-Skor; Analitik Hiyerarsik Surec; Dogrusal Kombinasyon Teknigi Determination of Soil Erosion Hazard with Integrated Multi-Criteria Assessment Using Remote Sensing, Geography Information System and Statistical Approaches; A Case Study in Ankara-Guvenc Basin Abstract: The main aim of this study is to determine erosion risk classes and to generate their map in Ankara Guvenc Basin using geographic information system (GIS), remote sensing (RS) and statistical approaches with method of multi-criteria decision-making. This study basin covers about 17 km². In order to determine erosion risk classes, the seven criteria (structure, soil depth, precipitation, land use, elevation, slope and vegetation) which affect on the occurrence of soil erosion and their sub-criteria were determined.  1:25.000-scale digital soil map and topographic map were used. In addition to that, Landsat-7 satellite images were used to determine the land use and vegetation cover. Analytical Hierarchy Process technique was used to determine the priorities of the each criteria while, the Z-Score statistical analysis techniques was used to determine the priorities of the sub-criteria. Finally, Linear Combination Technique was used to determine classes of erosion risk classes. According to the obtained results, 45% of the basin area is identified as very severe erosion risk. Key Words: Determining erosion risk; Z-score; Analytical Hierarchy Process; Linear Combination Technique
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引用次数: 1
Genotypic Variation Among Sorghum Cultivars for Seed Vigor, Salt and Drought Stresses 高粱品种种子活力、盐胁迫和干旱胁迫的基因型变异
4区 农林科学 Pub Date : 2017-09-06 DOI: 10.15832/TBD.34634
S. Avcı, O. İleri, M. Kaya
This study was conducted to determine genotypic variation among sorghum cultivars grown under similar ecological conditions for seed vigor, NaCl and drought stresses. A seedling survival test was also performed in a pod experiment to investigate genotypic differences in terms of sorghum drought tolerance. In seven sorghum cultivars, seed vigor was determined using electrical conductivity and accelerated aging tests, and germination rate and seedling growth of these cultivars were evaluated under salt (NaCl) and drought stress induced by PEG 6000 at water potentials of 0.0, 1.8, 3.6, 7.2 and 10.8 bar. The results showed that seed vigor differed among sorghum cultivars and the highest correlation coefficients were determined between laboratory emergence and accelerated aging (r=0.967**), and between germination and electrical conductivity (r=-0.873**). Electrical conductivity found a suitable test for determining germination potential of sorghum seed lots while the best method predicting emergence percentage was accelerated aging. Germination was delayed in both NaCl and PEG solutions. Germination, root length and shoot length were higher, whereas mean germination time was lower, in NaCl solution compared to in PEG solution at the same water potential. The most and least vigorous seeds were observed in the Akdari and Gozde 80 cultivars, which displayed the lowest germination percentages in NaCl and PEG solutions. From this study, it could be concluded that seed vigor is not a reliable indicator of germination performance under salt and drought stress conditions and that genetic differences may play an important role in stress tolerance.
本研究旨在研究在相似生态条件下不同高粱品种种子活力、NaCl胁迫和干旱胁迫的基因型差异。在荚果试验中进行了幼苗存活试验,以研究高粱抗旱性的基因型差异。采用电导率法和加速老化法测定了7个高粱品种的种子活力,并在水势为0.0、1.8、3.6、7.2和10.8 bar的NaCl和干旱胁迫下,对这些品种的种子发芽率和幼苗生长进行了评价。结果表明,不同品种高粱种子活力存在差异,其中萌发与加速老化的相关系数最高(r=0.967**),萌发与电导率的相关系数最高(r=-0.873**)。电导率法是测定高粱种子萌发势的最佳方法,而加速老化法是预测出苗率的最佳方法。在NaCl和PEG溶液中萌发均延迟。在相同水势条件下,NaCl溶液比PEG溶液的萌发率、根长和茎长高,平均萌发时间短。Akdari和Gozde 80的种子活力最强,在NaCl和PEG溶液中的发芽率最低。由此可见,种子活力并不是盐胁迫和干旱胁迫条件下发芽性能的可靠指标,遗传差异可能在胁迫耐受性中起重要作用。
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引用次数: 1
The comparative effects of microtubules in TUB-GFP and MBD-GFP Arabidopsis thaliana exposed to UV-B Radiation UV-B辐射下TUB-GFP和MBD-GFP拟南芥微管的比较效应
4区 农林科学 Pub Date : 2017-09-06 DOI: 10.15832/TBD.29540
D. Ma, Huize Chen, R. Han
In the present study, we used the TUB-GFP and MBD-GFP of the Arabidopsis thaliana , compared with effects of the plant height and primary root length, and changes of microtubules dynamics by confocal laser scanning microscope after UV-B radiation. It was shown that UV-B radiation had the same damaged effects on the transgenic lines, compared with wild type, and the transgenic lines were more sensitive for UV-B. MBD-GFP Arabidopsis thaliana could be better adapted to the changes with UV-B treatment. Therefore, these results indicated that UV-B could inhibit the growth and the development of transgenic Arabidopsis thaliana , and the inhibition effects might result from changes in microtubules.
本研究以拟南芥(Arabidopsis thaliana)为材料,利用共聚焦激光扫描显微镜,比较了UV-B辐射对拟南芥株高、初生根长以及微管动力学的影响。结果表明,与野生型相比,转基因株系对UV-B辐射具有相同的损伤效应,且转基因株系对UV-B更为敏感。MBD-GFP拟南芥能更好地适应UV-B处理的变化。因此,这些结果表明,UV-B可以抑制转基因拟南芥的生长发育,其抑制作用可能与微管的变化有关。
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引用次数: 1
Seasonal Carbohydrate Changes in the Leaves of Some Satsuma Cultivars on Different Rootstocks 不同砧木上几种甜瓜品种叶片碳水化合物的季节性变化
4区 农林科学 Pub Date : 2017-09-05 DOI: 10.15832/ANKUTBD.447710
E. Yıldız, M. Kaplankıran, T. H. Demirkeser, C. Toplu
The seasonal changes of carbohydrate content were monitored in the leaves of ‘Okitsu’, ‘Clausellina’, and ‘Silverhill’ satsuma cultivars budded on the sour orange (Citrus aurantium L. var. Yerli), Carrizo and Troyer citranges (Poncirus trifoliata Raf. x Citrus sinensis Osb. var. Troyer and Carrizo) during the 2010 and 2011 seasons. The seasonal changing trends of leaf soluble sugar contents of 3 satsuma cultivars budded on different rootstocks were similar. The sucrose was the most abundant soluble sugar. Carbohydrate contents had a maximum level at dormant period, and remained at low level during summer. In this period, the sharp decrease in leaf total carbohydrate concentration of ‘Okitsu’, ‘Clausellina’ and ‘Silverhill’ occurred in April with a rate of 27.9%, 22.6% and 21.0%, respectively. Accumulation of carbohydrate in leaves generally started after mid summer and continued until the end of year. The rootstocks were found to be not significant for total soluble sugar, starch and total carbohydrate concentration in most of the months. The cultivars had a significant effect on carbohydrate fractions of leaves compared to rootstocks. The scion-rootstock combination was significant on total carbohydrate concentration of leaves in all months investigated.
研究了在酸橙(Citrus aurantium L. var. Yerli)、卡里佐(Carrizo)和Troyer citranranges (Poncirus trifoliata Raf)上发芽的‘Okitsu’、‘Clausellina’和‘Silverhill’三个品种的叶片碳水化合物含量的季节变化。x Citrus sinensis Osb。var. Troyer和Carrizo)在2010年和2011年的季节。不同砧木出芽的3个品种叶片可溶性糖含量的季节变化趋势相似。蔗糖是最丰富的可溶性糖。碳水化合物含量在休眠期最高,在夏季保持较低水平。在此期间,‘Okitsu’、‘Clausellina’和‘Silverhill’叶片总碳水化合物浓度在4月份急剧下降,降幅分别为27.9%、22.6%和21.0%。叶片碳水化合物的积累一般在仲夏之后开始,一直持续到年底。在大部分月份,砧木的总可溶性糖、淀粉和总碳水化合物含量均无显著差异。与砧木相比,品种对叶片碳水化合物组分的影响显著。接穗-砧木组合对叶片总碳水化合物浓度影响显著。
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引用次数: 2
期刊
Tarim Bilimleri Dergisi-journal of Agricultural Sciences
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