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Impacts of salinity, yeast extract and drought treatments on Silybum marianum L. seed-silymarin content and constituents 盐度、酵母浸膏和干旱处理对水飞蓟种子水飞蓟素含量和成分的影响
Pub Date : 2020-01-01 DOI: 10.5455/egyjebb.20200605025834
Mahmoud Abdelhaak, Dina Gad
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引用次数: 1
Floristic composition, Life forms and Phytogeography of Al-Hashr Mountain, Jazan region, SW Saudi Arabia 沙特阿拉伯西南部吉赞地区Al-Hashr山的植物区系组成、生命形式和植物地理
Pub Date : 2019-01-01 DOI: 10.5455/EGYJEBB.20190301092631
Lamiaa Farouq Shalabiı, Y. Masrahi
The Floristic composition, life forms and phytogeographical iaffinities were conducted in Al-Hashr Mountain, Southwestern iSaudi Arabia. In total, 116 plant species belonging to 95 genera iand 47 families were collected and identified. Asteraceae and iPoaceae were the most important families in terms of species irichness. The life forms spectrum was dominated by ichamaephytes, while the remainders were therophytes and iphanerophytes, denoting phytoclimate of semi-arid mountainous ihabitat with topographical variation refuge many microhabitats, as iwell as anthropogenic factors. Due to its location, the study area, ias a part of southwestern Saudi Arabia, is a meeting place of isome phytogeographical regions of Holarctic and Palaeotropic, iwith high affinity to Sudano-Zambezian phytochorion, as well as ihigh degree of endemism of South Arabian. i
对沙特西南部Al-Hashr山的植物区系组成、生命形式和植物地理亲缘关系进行了研究。共收集鉴定植物116种,隶属于47科95属。在物种丰富度方面,菊科和菊科是最重要的科。生命形态谱以蕨类植物为主,以蕨类植物和蕨类植物为主,表明该地区为半干旱山地生境,地形多变,微生境较多,且受人为因素影响。由于所处的地理位置,研究区位于沙特阿拉伯西南部,是全北极和古向异性植物地理区域的交汇处,与苏丹-赞比西亚植物群落高度亲和,南阿拉伯特有程度高。我
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引用次数: 5
Seed priming in natural weed extracts represents a promising practice for alleviating lead stress toxicity 种子引种在天然杂草提取物代表了一个有希望的做法,以减轻铅胁迫毒性
Pub Date : 2019-01-01 DOI: 10.5455/egyjebb.20191223054507
Sherien E. Sobhy, K. Allah, Essam Kassem, E. Hafez, N. Sewelam
Soil contamination with lead (Pb) represents a common heavy metal stress for cultivated plants. In the current study, effects of Pb stress on wheat (Triticum aestivum) plants were evaluated, and alleviating roles of purslane and chard natural weed extracts were assessed. The results showed that Pb treatment (100 mM) significantly reduced the growth of wheat seedlings and diminished plant yield parameters. Moreover, Pb stress inhibited photosynthetic activity measured as Fv/Fm. The levels of various stress markers increased under Pb stress as was verified by the increased electrolyte leakage, malondialdehyde (MDA) content, H2O2 and .OH radical content. Pb stress increased the activity of peroxidase (POX), catalase (CAT), superoxide dismutase (SOD), as well as raised the content of the antioxidant compound ascorbic acid. A remarkable induction in some genes was reported under Pb stress. The ameliorative role achieved by seed priming of wheat in purslane and chard weed extracts was demonstrated by the increase in growth parameters in seedling and yield stages. In the meantime, the priming treatments increased the Fv/Fm value. In addition, the activities of the antioxidant enzymes POX, CAT and SOD were reduced by seed priming. Data presented in the current work provide strong evidence that natural weed extracts are efficient to alleviate damaging effects of abiotic challenges such as heavy metal stress imposed by high soil Pb concentrations, and thus possibly could represent a valuable ecofriendly and commercial values for plant farmers.
土壤铅污染是栽培植物常见的重金属胁迫。本研究评价了铅胁迫对小麦(Triticum aestivum)植株的影响,并评价了马齿苋和甜菜天然杂草提取物的缓解作用。结果表明,Pb处理(100 mM)显著降低了小麦幼苗的生长,降低了植株的产量参数。此外,Pb胁迫抑制了光合活性(Fv/Fm)。在Pb胁迫下,电解质泄漏量、丙二醛(MDA)含量、H2O2和。oh自由基含量均增加。铅胁迫提高了过氧化物酶(POX)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)的活性,提高了抗氧化剂抗坏血酸的含量。据报道,Pb胁迫对部分基因有显著的诱导作用。小麦引种对马齿苋和甜菜杂草提取物的改良作用可以通过提高幼苗期和产出期的生长参数来证明。同时,启动处理增加了Fv/Fm值。此外,种子诱导降低了抗氧化酶POX、CAT和SOD的活性。本研究提供的数据有力地证明,天然杂草提取物可以有效地缓解高土壤铅浓度造成的重金属胁迫等非生物挑战的破坏性影响,因此可能对植物种植者具有重要的生态和商业价值。
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引用次数: 6
Molecular and cytogenetic assessment of zinc nanoparticles on Vicia faba plant cells. 锌纳米颗粒对蚕豆细胞的分子和细胞遗传学研究。
Pub Date : 2019-01-01 DOI: 10.5455/EGYJEBB.20181108065044
H. Mahmoud
Abstract Nanotechnology is an emerging multidisciplinary field with a wide range of applications in agriculture, cancer therapy, targeted drug delivery and cosmetic industry. Nevertheless, unspecified release of nanoparticles (NPs) into the ecosystem has raised global concern about their potential toxic effects. In the present study, the effect of different concentrations of ZnO NPs were evaluated on molecular DNA level, on mitotic and meiotic division of Vicia faba plant. The data obtained using ISSR assay showed change in bands number in all tested samples. The highest polymorphic bands recorded are 36 and 34 bands at samples treated with 50.00 and 37.50 μg/mL of ZnO NPs respectively, as compared with untreated plants. In addition to appearance and disappearance of bands in ISSR profiles, a decrease in genetic template stability (GTS) values was recorded with increasing the concentration. The GTS value recorded 58.62% with the highest concentration (50.00 μg/mL); while recorded 67.82% in samples treated with the lowest concentration (6.25 μg/mL) of ZnO NPs. High GTS value indicates that the genome is less prone to alterations in its DNA, whereas low GTS value indicates greater chances of DNA alteration. On the other hand, the cytotoxic effect of ZnO NPs as indicated by inhibiting mitotic and meiotic division and production of chromosomal aberrations such as chromosome breaks was confirmed by their effect on DNA profiles in Vicia faba plant. This study revealed that higher concentrations of Zinc oxide nanoparticles (ZnO NPs) produce genotoxic effects which indirectly can cause health risks to the human populations. Further studies focused over elucidating the fate and behaviour of ZnO-NPs in complex environmental matrices are needed to safeguard ecosystem functioning as well as human kind. However, to the best of our understanding no molecular studies of ZnO-NPs on Vicia faba L. has been reported.
纳米技术是一个新兴的多学科领域,在农业、癌症治疗、靶向给药和化妆品等领域有着广泛的应用。然而,纳米颗粒(NPs)释放到生态系统中引起了全球对其潜在毒性影响的关注。本文研究了不同浓度氧化锌NPs对蚕豆分子DNA水平、有丝分裂和减数分裂的影响。利用ISSR法获得的数据显示,所有测试样品的条带数都发生了变化。氧化锌NPs浓度为50.00 μg/mL和37.50 μg/mL时,与未处理的植株相比,最多的多态性条带分别为36条和34条。除了ISSR谱带的出现和消失外,遗传模板稳定性(GTS)值也随着浓度的增加而降低。GTS值为58.62%,最高浓度为50.00 μg/mL;最低氧化锌NPs浓度(6.25 μg/mL)处理的样品中占67.82%。GTS值高表明基因组不易发生DNA改变,而GTS值低表明DNA改变的可能性更大。另一方面,氧化锌NPs对蚕豆DNA谱的影响证实了氧化锌NPs的细胞毒性作用,氧化锌NPs通过抑制有丝分裂和减数分裂分裂以及染色体畸变(如染色体断裂)的产生而得到证实。本研究表明,高浓度氧化锌纳米颗粒(ZnO NPs)可产生遗传毒性效应,间接对人类健康造成危害。进一步研究ZnO-NPs在复杂环境基质中的命运和行为是保护生态系统功能和人类的必要条件。然而,据我们所知,没有关于ZnO-NPs在蚕豆上的分子研究报道。
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引用次数: 5
Allelopathic effect of the green macroalgae Ulva fasciata (Delile) on potentially harmful algal bloom forming species 绿藻对潜在有害藻华形成物种的化感作用
Pub Date : 2019-01-01 DOI: 10.5455/EGYJEBB.20190505044917
Shimaa M. El Shafay, A. Shady, L. Mohamed, Shimaa Hosny, W. Labib
The present study investigated the potential allelopathic effects of the living green marine macroalga Ulva fasciata growth of several marine microalgae. Skeletonema costatum (Bacillariophyta, centric diatom), Nitzschia longissima (pennate diatom), Scrippsiella trochoidea & Alexandrium minutum (Dinophyta), and Heterosigma akashiwo (Raphidophyta) all being previously identified as bloom forming species in Alexandria coastal water, Egypt. The results showed reduction of microalgal growth as a result of treatment with fresh thalli compared to the respective control treatments. In addition, macroalgal growth increased with the increasing of treatment time in a dependent way. The degree of dominance and concentrations of fatty acids oscillated in all treatments. The fatty acids composition showed 11 pattern of Fatty acids (9 saturated& 2 unsaturated). The major saturated acids arranged by decreasing order are C15.0, C14.0, C16.0 and C13.0.
本研究研究了活的绿色海洋巨藻对几种海洋微藻生长的潜在化感作用。骨骨藻(硅藻门,中心硅藻),长硅藻(pennate硅藻),trochoidea Scrippsiella & Alexandrium minutum(甲藻门)和Heterosigma akashiwo(刺藻门)都曾被确定为埃及亚历山大沿海水域的水华形成物种。结果表明,与相应的对照处理相比,新鲜菌体处理的结果是微藻生长减少。大藻的生长随处理时间的延长呈依赖关系。脂肪酸的优势度和浓度在所有处理中都有波动。脂肪酸组成呈现11种模式(9种饱和,2种不饱和)。饱和酸由大到小依次为C15.0、C14.0、C16.0和C13.0。
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引用次数: 1
Herbicidal potential of desert plants Artemisia judaica L., Asphodelus microcarpus Salzm. and Viv. and Solanum nigrum L. against Portulaca oleracea and Phalaris minor
Pub Date : 2019-01-01 DOI: 10.5455/EGYJEBB.20190507020508
Hanan Ahmed Hashem, Abdel Rahman, H. Kassem, N. F. Aziz
The aim of the present work was to evaluate the allelopathic effect of different concentrations (25, 50 and 100 µg/ml) of root exudates and aqueous leaf extracts of three desert plants (Artemisia judaica, Asphodelus microcarpus and Solanum nigrum) on two weed plants (Portulaca oleracea and Phalaris minor). In laboratory experiment, the three studied plants possessed herbicidal effects that significantly inhibited germination, seedling growth and chlorophyll content of P. oleracea and P. minor; the inhibitory effect was proportional to the concentrations applied. Artemisia judaica and A. microcarpus were the most effective root exudates against P. oleracea while A. microcarpus was the most effective against P. minor compared to the other examined root exudates. Meanwhile, A. judaica was proved to be the most effective leaf extract against P. oleracea and P. minor followed by A. microcarpus and then S. nigrum. A number of phenols and flavonoids with known allelopathic potential was identified in the leaf extracts of the three studied plants and could explain their inhibitory effect on P. minor and P. oleracea plants. Generally, the aqueous leaf extracts showed greater allelopathic effects than the root exudates. However, both root exudates and aqueous leaf extracts of the three tested plants are attractive to be utilized in bio- herbicidal development program towards active weed management.
研究了不同浓度(25、50和100µg/ml)的荒漠植物(黄花蒿、小菖蒲和龙葵)根渗出液和叶片水提液对两种杂草(马齿苋和蝴蝶兰)的化感作用。在室内试验中,3种植物均具有显著抑制马齿苋和小马齿苋种子萌发、幼苗生长和叶绿素含量的除草作用;抑制效果与应用浓度成正比。与其他根分泌物相比,青蒿和小菖蒲对甘蓝根腐病的防治效果最好,而小菖蒲对甘蓝根腐病的防治效果最好。同时,对甘蓝和小甘蓝的防治效果最好的是犹太麻叶提取物,其次是小仙人掌叶提取物,最后是黑葡萄叶提取物。在这三种植物的叶片提取物中发现了许多具有化感作用的酚类和黄酮类化合物,可以解释它们对小叶姜和甘蓝的抑制作用。一般来说,水浸叶提取物的化感作用比根渗出液大。然而,三种被试植物的根分泌物和叶水提取物都有可能在生物除草开发计划中用于主动杂草管理。
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引用次数: 3
Synthesis, characterization and biological activities of copper nanoparticles 铜纳米颗粒的合成、表征及生物活性研究
Pub Date : 2019-01-01 DOI: 10.5455/egyjebb.20191208055126
E. A. M. Ali
Nanotechnology is the most advanced research area in the present era. Copper nanoparticles displayed a significant role in many biological fields. The aim of the present study is to synthesize, characterize and study the biological activities of copper nanoparticles. Copper nanoparticles were produced from copper nitrate by chemical reduction method using isopropyl alcohol and cetyl trimethylammonium bromide (CTAB). Synthesized particles were characterized by fourier transform infrared (FTIR) spectrophotometer and transmission electron microscopic (TEM) analysis. Spherical particles were produced with size range 5-13 nm. Antimicrobial activities of both copper nanoparticles and copper nitrate precursor were tested against Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Candida albicans, Penicillium expansum, Staphylococcus aureus, Streptococcus faecalis, Streptococcus mutans, Escherichia coli, Neisseria gonorrhoeae and Pseudomonas aeruginosa. Both copper nanoparticles and copper nitrate precursor showed higher antibacterial activities than antifungal activities. Copper nanoparticles and copper nitrate precursor showed antioxidant activity relatively close to each other, with efficient antioxidant activity reached to 95.42% at 500 µg/ml of copper nanoparticles. Copper nanoparticles revealed higher significant cytotoxic effects than copper nitrate precursor. Production of copper nanoparticles by using chemical reduction method was simple, unexpensive and fast. Copper nanoparticles exhibited significant biological activities.
纳米技术是当今时代最先进的研究领域。铜纳米颗粒在许多生物领域显示出重要的作用。本研究的目的是合成、表征和研究铜纳米颗粒的生物活性。以硝酸铜为原料,以异丙醇和十六烷基三甲基溴化铵(CTAB)为原料,采用化学还原法制备纳米铜。采用傅里叶变换红外(FTIR)分光光度计和透射电子显微镜(TEM)对合成颗粒进行了表征。制备出粒径为5 ~ 13 nm的球形颗粒。研究了铜纳米颗粒和硝酸铜前体对黄曲霉、烟曲霉、黑曲霉、白色念珠菌、扩张青霉、金黄色葡萄球菌、粪链球菌、变形链球菌、大肠杆菌、淋病奈瑟菌和铜绿假单胞菌的抑菌活性。纳米铜和硝酸铜前体均表现出较强的抑菌活性。纳米铜与硝酸铜前驱体的抗氧化活性较为接近,在500 µg/ml时,纳米铜的有效抗氧化活性达到95.42%。纳米铜的细胞毒作用明显高于硝酸铜前体。化学还原法制备纳米铜具有简单、经济、快速等优点。铜纳米颗粒具有显著的生物活性。
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引用次数: 0
Histological and Molecular Detections of Peronospora variabilis Gäum Oospores in Seeds of Quinoa (Chenopodium quinoa L.) 藜麦种子中Peronospora variabilis Gäum卵孢子的组织学和分子检测
Pub Date : 2019-01-01 DOI: 10.5455/egyjebb.20190620115158
Elhamy M. El-Assiuty, E. Taha, Z. Fahmy, G. M. Fahmy
The oospores of Perosnospora variabilis Gaum, the causal agent of downy mildew of quinoa (Chenopodium quinoa L.), were detected histologically in the perianth of quinoa seed, the pericarp, testa, perisperm, and the cotyledons of the embryo. The histological detections of oospores were confirmed by the polymerase chain reaction (PCR) using the DNA extracted from the perianth and all of the seed parts. The percentage occurrence of oospores in examined seed samples was high in the perianth (90%) followed by the seed coat (87%) whereas, the lowest percentages were found in the embryo (3%) and the perisperm (2%).
对藜麦霜霉病病原菌Perosnospora variabilis Gaum的卵孢子在藜麦种子的花被、果皮、种皮、外种皮和胚子叶中进行了组织学检测。从花被和种子各部分提取DNA,用聚合酶链反应(PCR)证实了卵孢子的组织学检测结果。结果表明,卵孢子在被花被中所占比例最高(90%),其次是种皮(87%),而在胚(3%)和外种皮中所占比例最低(2%)。
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引用次数: 5
Toxic materials phytoremediation potential of four common trees in Saudi Arabia 沙特阿拉伯四种常见树种有毒物质的植物修复潜力
Pub Date : 2019-01-01 DOI: 10.5455/egyjebb.20190225102702
M. Khazan, Reham M.Al-Zlabani
Heavy metals are the most serious environmental pollutants in the current time as a result of industrial development in several countries. Their pollution is poisoning threat for human, animal and plant life because toxic metals cause several serious ailments. Many techniques have been emerged for the elimination of heavy metal contamination for the environment. Either physical or chemical ones, have limitations such as high cost, long time, logistical problems and mechanical complexity. Phytoremediation alternative solution can be used for heavy metal remediation process because of its advantages as a cost-effective, efficient, and eco-friendly technology based on the use of metal-accumulating plants. Many plant species have a high potential as heavy metals bioaccumulators and can be used for their phytoremediation process. This review focus on the common phytoremediation mechanisms and the role of four street-tree species (Moringa oleifera, Azadirachta indica, Lantana camara and Conocarpus erectus) commonly cultivated throughout the Kingdom of Saudi Arabia (KSA) in phytoremediating various pollutants. Trees description, habitat of growth and their potential to phytoremediate different heavy metals would be discussed.
随着各国工业的发展,重金属已成为当今世界最严重的环境污染物。它们的污染是对人类、动物和植物生命的中毒威胁,因为有毒金属会导致几种严重的疾病。为了消除重金属对环境的污染,已经出现了许多技术。无论是物理的还是化学的,都有成本高、时间长、物流问题和机械复杂性等局限性。植物修复替代方案是一种基于重金属富集植物的经济、高效、环保的重金属修复技术。许多植物作为重金属的生物蓄积器具有很高的潜力,可用于植物修复过程。本文综述了沙特阿拉伯王国常见的四种街树(Moringa oleifera, Azadirachta indica, Lantana camara和Conocarpus erectus)在植物修复各种污染物中的作用。讨论了树木描述、生长生境及其修复不同重金属的潜力。
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引用次数: 3
Seed and germination traits of the summer weed Trianthema portulacastrum L. 夏草马齿苋种子及萌发特性研究。
Pub Date : 2019-01-01 DOI: 10.5455/EGYJEBB.20190618082647
G. M. Fahmy, S. A. Moussa, H. Farrag, R. A. A. Rehem
Trianthema portulacastrum L. is one of the problematic terrestrial summer weeds. It competes with various agricultural crops. The present investigation aimed to investigate the traits, which enable the seeds to disperse, germinate, grow and adapt to habitat conditions. The plant produces large number (1931 seeds) of small seeds (1.08 mg seed-1). Scanning electron microscopy of the seed testa revealed that the anticlinal walls of the epidermal cells were penta-hexagonal, and highly raised, while the periclinal walls were sunken. This pattern leads to the porous appearance of the testa. The effects of temperature on seed germination were monitored under continuous light and continuous dark conditions. Under light conditions, the percentages of seed germination were significantly higher than at the dark conditions. Higher temperatures of 35, 40 and 45 °C promoted the seed germination. In the temperature range from 30 to 45 °C, the plumule lengths of the seedlings germinated in the light conditions were smaller than those grown under dark conditions. Under all conditions, the length of radicle was higher than the plumule. The traits of seeds, germination, and seedlings grown under experimental conditions are discussed in terms of the seed dispersal and potential adaptations of seedlings to the environment.
马齿苋(Trianthema portulacastrum L.)是一种有问题的陆生夏季杂草。它与各种农作物竞争。本研究旨在研究使种子能够传播、发芽、生长和适应生境条件的性状。该植物产生大量(1931粒)小种子(1.08毫克种子-1)。种子种皮扫描电镜显示,表皮细胞背斜壁呈五六边形,高度凸起,而周周壁凹陷。这种模式导致睾丸的多孔外观。在连续光照和连续黑暗条件下监测温度对种子萌发的影响。光照条件下种子发芽率显著高于黑暗条件。35℃、40℃和45℃的高温对种子萌发有促进作用。在30 ~ 45℃的温度范围内,光照条件下萌发的幼苗的胚珠长度小于光照条件下萌发的幼苗。在所有条件下,胚根长度均大于胚珠长度。从种子传播和幼苗对环境的潜在适应的角度讨论了实验条件下种子、萌发和幼苗的特性。
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引用次数: 4
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THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)
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