Pub Date : 2019-01-01DOI: 10.5455/egyjebb.20191031071835
Hala Nosair
Although pesticides are extensively utilized against plant pests and diseases, the unsolicited impacts on our non-target organisms must be addressed. In this work, we investigate the DNA damage as well as the change in protein content of palm seedlings caused by, cypermethrin (CM) as an insecticide. Genetic differences of seedlings treated with various concentrations of CM compared to the control were detected by applying SDS-PAGE analysis and ISSR markers. Seedlings were treated for one week by the application of four different CM concentrations in the range (18.75 -150 ppm). SDS-PAGE revealed large changes in the protein patterns such as the disappearance of some bands at higher CM concentrations and the appearance of a new number of bands reaching maximum at of the lowest CM concentration (0.25 of the recommended). ISSR showed induced variations in banding profiles of all treated seedlings compared to control. The obtained DNA polymorphisms have indicated the genotoxic effects of CM for all concentrations used. The data generated in this study show not only the effect of CM on the treated seedlings but also its extended effects on the next offspring.
{"title":"Molecular Response of Palm Seedlings against Cypermithrin","authors":"Hala Nosair","doi":"10.5455/egyjebb.20191031071835","DOIUrl":"https://doi.org/10.5455/egyjebb.20191031071835","url":null,"abstract":"Although pesticides are extensively utilized against plant pests and diseases, the unsolicited impacts on our non-target organisms must be addressed. In this work, we investigate the DNA damage as well as the change in protein content of palm seedlings caused by, cypermethrin (CM) as an insecticide. Genetic differences of seedlings treated with various concentrations of CM compared to the control were detected by applying SDS-PAGE analysis and ISSR markers. Seedlings were treated for one week by the application of four different CM concentrations in the range (18.75 -150 ppm). SDS-PAGE revealed large changes in the protein patterns such as the disappearance of some bands at higher CM concentrations and the appearance of a new number of bands reaching maximum at of the lowest CM concentration (0.25 of the recommended). ISSR showed induced variations in banding profiles of all treated seedlings compared to control. The obtained DNA polymorphisms have indicated the genotoxic effects of CM for all concentrations used. The data generated in this study show not only the effect of CM on the treated seedlings but also its extended effects on the next offspring.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76927377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5455/EGYJEBB.20181206084342
T. M. Abdel-Ghany, M. Abboud, Mohamed M. Alawlaqi, A. M. Shater
Decolorization of congo red with growing and dead biomass of thermophilic Aspergillus fumigatus was studied. The biosorption percent by dead biomass increases with increasing pH up to 6 at dye initial concentration 10,60 and 70 mg/100ml , and up to pH7 at dye initial concentration 20,30,40,50 and 60 mg/100ml. The mount of congo red biosorbed onto dead biomass increases with increasing temperature from 10 to 30°C then decreases at 40 °C and sharply at 50°C. Increase in dead biomass concentration above 0.8 (g/100ml) did not effective in biosorption of congo red. Maximum decolourization was observed at 180min of contact time. The study concluded that dead fungal biomass possesses various advantages such as absence of nutrient needs, therefore the findings offer potential for the development of a cost effective for biosorption of congo red.
{"title":"Dead biomass of thermophilic Aspergillus fumigatus for Congo red biosorption","authors":"T. M. Abdel-Ghany, M. Abboud, Mohamed M. Alawlaqi, A. M. Shater","doi":"10.5455/EGYJEBB.20181206084342","DOIUrl":"https://doi.org/10.5455/EGYJEBB.20181206084342","url":null,"abstract":"Decolorization of congo red with growing and dead biomass of thermophilic Aspergillus fumigatus was studied. The biosorption percent by dead biomass increases with increasing pH up to 6 at dye initial concentration 10,60 and 70 mg/100ml , and up to pH7 at dye initial concentration 20,30,40,50 and 60 mg/100ml. The mount of congo red biosorbed onto dead biomass increases with increasing temperature from 10 to 30°C then decreases at 40 °C and sharply at 50°C. Increase in dead biomass concentration above 0.8 (g/100ml) did not effective in biosorption of congo red. Maximum decolourization was observed at 180min of contact time. The study concluded that dead fungal biomass possesses various advantages such as absence of nutrient needs, therefore the findings offer potential for the development of a cost effective for biosorption of congo red.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80901774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5455/egyjebb.20190722115434
Walaa A. Rayan
Alterations in the DNA sequences are very useful for the unique markers development, which could be utilized as a barcoding of DNA for different plants. The barcoding of DNA is used for identification of living organisms and provides extra and integral support to identify the morpho-species, because it characterized by a reproducible, rapid and economic tool. This study was conducted to analyze the phylogenetic relationships between two species of the genus Moringa; M. oleifera and M. Peregrina, using DNA barcodes. In Egypt only two Moringa species (M. oleifera and M. Peregrina) were cultivated. These two species (four Moringa genotypes) were commonly used in the traditional medicine. To authenticate the different genotypes, rbcL regions were evaluated. DNA barcode regions were amplified using universal primers. The length of rbcL barcoding region varied between 638 bp in M. oleifera (II) collected from El-Suis governorate (Egypt) to 663 bp in M. peregrina from Giza (III), with a mean of 649.75 bp. In all investigated species, the percentage of GC content found to be nearly 44.01% and the span aligned sequences around 638-663 nucleotides. The number of variable sites within the sequences of the four Moringa genotypes was found to be 46 sites (11 transitions, 15 transversions and 20 indel). A phylogentic tree based on rbcL barcoding was constructed. Three clusters were shown, the first contained Moringa oleifera (R2) and the second contained Moringa oleifera (R1) and Moringa peregrina (R3). While the third cluster contained Moringa peregrina (R4). Several studies have reported similar observations. The results demonstrate that the analysis of these DNA barcode sequences is a reliable method for distinguishing between four Moringa genotypes.
{"title":"Evaluating the efficiency of DNA Barcode rbcL for detection of genetic relationships between four Moringa spp. genotypes","authors":"Walaa A. Rayan","doi":"10.5455/egyjebb.20190722115434","DOIUrl":"https://doi.org/10.5455/egyjebb.20190722115434","url":null,"abstract":"Alterations in the DNA sequences are very useful for the unique markers development, which could be utilized as a barcoding of DNA for different plants. The barcoding of DNA is used for identification of living organisms and provides extra and integral support to identify the morpho-species, because it characterized by a reproducible, rapid and economic tool. This study was conducted to analyze the phylogenetic relationships between two species of the genus Moringa; M. oleifera and M. Peregrina, using DNA barcodes. In Egypt only two Moringa species (M. oleifera and M. Peregrina) were cultivated. These two species (four Moringa genotypes) were commonly used in the traditional medicine. To authenticate the different genotypes, rbcL regions were evaluated. DNA barcode regions were amplified using universal primers. The length of rbcL barcoding region varied between 638 bp in M. oleifera (II) collected from El-Suis governorate (Egypt) to 663 bp in M. peregrina from Giza (III), with a mean of 649.75 bp. In all investigated species, the percentage of GC content found to be nearly 44.01% and the span aligned sequences around 638-663 nucleotides. The number of variable sites within the sequences of the four Moringa genotypes was found to be 46 sites (11 transitions, 15 transversions and 20 indel). A phylogentic tree based on rbcL barcoding was constructed. Three clusters were shown, the first contained Moringa oleifera (R2) and the second contained Moringa oleifera (R1) and Moringa peregrina (R3). While the third cluster contained Moringa peregrina (R4). Several studies have reported similar observations. The results demonstrate that the analysis of these DNA barcode sequences is a reliable method for distinguishing between four Moringa genotypes.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"45 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85432920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.5455/EGYJEBB.20181126081938
Hanan Temerk
{"title":"Isolation and characterization of Haloferax mucsum from Red sea saltern as an halobacterial Archea capable of growing on Naphtaline as an aromatic compounds.","authors":"Hanan Temerk","doi":"10.5455/EGYJEBB.20181126081938","DOIUrl":"https://doi.org/10.5455/EGYJEBB.20181126081938","url":null,"abstract":"","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72707682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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