Pub Date : 2019-01-01DOI: 10.5455/EGYJEBB.20190111081336
Gouda T. M. Dawoud, Mohamed E. A.Dawoud, Yousef E. Abdel Mawgood
ABSTRACT Susceptibility of some gastrointestinal tract (GIT) bacterial-pathogens to combinations of MRSA-growth inhibiting antibiotics (MGIA), MRSA growth non-inhibiting antibiotics (MGNIA) and some plant extracts, separately or in groups were investigated. The results revealed that combinations from MGNIA (Gentamycin with Co trimoxazole), MGIA (Vancomycin with Ciprofloxacin) and MGNIA with MGIA (Gentamycin with Imipenem) were determinative for the growth of most tested GIT-pathogens. On the other hand, aerial shoots of Artimesia monosperma L (Am), Ocimum basilicum L (Ob), Origanum majorana L (Om), Salvia officinalis L (So) and Pelargonium graveolens (Pg) were applied collectively in a mixture (PM) and yield the most lethal effect on the tested pathogens (13.2 IZD). Further total plant mixture (TPM) Foeniculum vulgare (Fv) seed mixture (SM) with PM increased the determinative impact on pathogens growth and resulted in more susceptibility (17.3mm IZD). GC/MC analyses have shown that the major essential oils compounds of TM were β -Pinene 55 %, from (Am), Linalool 46%, Methyl chavicol 14.2% and 1, 8- cineole 12.3% from (Ob), terpinen-4-ol 31% and γ-Terpinene 14% from (Om), camphor 26.4 %, 1, 8- cineole 18% and α-thujone 14% from (So). Citronellol 40% from (Pg), Trans-anethole 66% and 1, 8- cineole12% from (Fv). Moreover, combination of TPM with Gentamicin and Imipenem (TPMGI) had a further upmost synergistic effect (25 mm IZD). The mode of action for TPMGI mixture ingredients may be resulted from the disruption of bacterial cell membrane, blocking of protein synthesis and out diffusion of cellular components.
摘要本文研究了部分胃肠道病原菌对MRSA生长抑制抗生素(MGIA)、MRSA生长非抑制抗生素(MGNIA)和部分植物提取物联合用药的敏感性。结果显示，MGNIA(庆大霉素与Co trimoxazole)、MGIA(万古霉素与环丙沙星)和MGNIA(庆大霉素与亚胺培南)联合对大多数gat病原菌的生长具有决定性作用。单精蒿(amtimesia monosperma L)、basilicum L (Ob)、牛头草(Origanum majorana L (Om))、鼠尾草(Salvia officinalis L (So))和天竺葵(Pelargonium graveolens (Pg))的空中芽混合施用(PM)对病原菌的致死效果最高(13.2 IZD)。此外，全植物混合物(TPM)、小茴香(Fv)混合种子(SM)与PM对病原菌生长的决定性影响更大，对病原菌的敏感性更高(17.3mm IZD)。GC/MC分析表明，天牛挥发油的主要挥发油成分为(Am)中β -蒎烯占55%，(Ob)中芳樟醇占46%，甲基chavicol占14.2%，1,8 -桉叶油素占12.3%，(Om)中松油烯-4-醇占31%，γ-松油烯占14%，(So)中樟脑占26.4%，1,8 -桉叶油素占18%，α-图琼占14%。香茅醇40%来自(Pg)，反式茴香醇66%来自(Fv)， 1,8 -桉叶精油12%来自(Fv)。此外，TPM联合庆大霉素和亚胺培南(TPMGI)具有进一步的最高协同效应(25 mm IZD)。TPMGI混合物成分的作用方式可能是由于破坏细菌细胞膜，阻断蛋白质合成和细胞成分的外扩散。
{"title":"Impact Action of Some Volatile Oils and Antibiotics on GIT-Pathogenic Bacteria","authors":"Gouda T. M. Dawoud, Mohamed E. A.Dawoud, Yousef E. Abdel Mawgood","doi":"10.5455/EGYJEBB.20190111081336","DOIUrl":"https://doi.org/10.5455/EGYJEBB.20190111081336","url":null,"abstract":"ABSTRACT Susceptibility of some gastrointestinal tract (GIT) bacterial-pathogens to combinations of MRSA-growth inhibiting antibiotics (MGIA), MRSA growth non-inhibiting antibiotics (MGNIA) and some plant extracts, separately or in groups were investigated. The results revealed that combinations from MGNIA (Gentamycin with Co trimoxazole), MGIA (Vancomycin with Ciprofloxacin) and MGNIA with MGIA (Gentamycin with Imipenem) were determinative for the growth of most tested GIT-pathogens. On the other hand, aerial shoots of Artimesia monosperma L (Am), Ocimum basilicum L (Ob), Origanum majorana L (Om), Salvia officinalis L (So) and Pelargonium graveolens (Pg) were applied collectively in a mixture (PM) and yield the most lethal effect on the tested pathogens (13.2 IZD). Further total plant mixture (TPM) Foeniculum vulgare (Fv) seed mixture (SM) with PM increased the determinative impact on pathogens growth and resulted in more susceptibility (17.3mm IZD). GC/MC analyses have shown that the major essential oils compounds of TM were β -Pinene 55 %, from (Am), Linalool 46%, Methyl chavicol 14.2% and 1, 8- cineole 12.3% from (Ob), terpinen-4-ol 31% and γ-Terpinene 14% from (Om), camphor 26.4 %, 1, 8- cineole 18% and α-thujone 14% from (So). Citronellol 40% from (Pg), Trans-anethole 66% and 1, 8- cineole12% from (Fv). Moreover, combination of TPM with Gentamicin and Imipenem (TPMGI) had a further upmost synergistic effect (25 mm IZD). The mode of action for TPMGI mixture ingredients may be resulted from the disruption of bacterial cell membrane, blocking of protein synthesis and out diffusion of cellular components.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"67 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79765707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5455/EGYJEBB.20181206084342
T. M. Abdel-Ghany, M. Abboud, Mohamed M. Alawlaqi, A. M. Shater
Decolorization of congo red with growing and dead biomass of thermophilic Aspergillus fumigatus was studied. The biosorption percent by dead biomass increases with increasing pH up to 6 at dye initial concentration 10,60 and 70 mg/100ml , and up to pH7 at dye initial concentration 20,30,40,50 and 60 mg/100ml. The mount of congo red biosorbed onto dead biomass increases with increasing temperature from 10 to 30°C then decreases at 40 °C and sharply at 50°C. Increase in dead biomass concentration above 0.8 (g/100ml) did not effective in biosorption of congo red. Maximum decolourization was observed at 180min of contact time. The study concluded that dead fungal biomass possesses various advantages such as absence of nutrient needs, therefore the findings offer potential for the development of a cost effective for biosorption of congo red.
{"title":"Dead biomass of thermophilic Aspergillus fumigatus for Congo red biosorption","authors":"T. M. Abdel-Ghany, M. Abboud, Mohamed M. Alawlaqi, A. M. Shater","doi":"10.5455/EGYJEBB.20181206084342","DOIUrl":"https://doi.org/10.5455/EGYJEBB.20181206084342","url":null,"abstract":"Decolorization of congo red with growing and dead biomass of thermophilic Aspergillus fumigatus was studied. The biosorption percent by dead biomass increases with increasing pH up to 6 at dye initial concentration 10,60 and 70 mg/100ml , and up to pH7 at dye initial concentration 20,30,40,50 and 60 mg/100ml. The mount of congo red biosorbed onto dead biomass increases with increasing temperature from 10 to 30°C then decreases at 40 °C and sharply at 50°C. Increase in dead biomass concentration above 0.8 (g/100ml) did not effective in biosorption of congo red. Maximum decolourization was observed at 180min of contact time. The study concluded that dead fungal biomass possesses various advantages such as absence of nutrient needs, therefore the findings offer potential for the development of a cost effective for biosorption of congo red.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80901774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5455/egyjebb.20190722115434
Walaa A. Rayan
Alterations in the DNA sequences are very useful for the unique markers development, which could be utilized as a barcoding of DNA for different plants. The barcoding of DNA is used for identification of living organisms and provides extra and integral support to identify the morpho-species, because it characterized by a reproducible, rapid and economic tool. This study was conducted to analyze the phylogenetic relationships between two species of the genus Moringa; M. oleifera and M. Peregrina, using DNA barcodes. In Egypt only two Moringa species (M. oleifera and M. Peregrina) were cultivated. These two species (four Moringa genotypes) were commonly used in the traditional medicine. To authenticate the different genotypes, rbcL regions were evaluated. DNA barcode regions were amplified using universal primers. The length of rbcL barcoding region varied between 638 bp in M. oleifera (II) collected from El-Suis governorate (Egypt) to 663 bp in M. peregrina from Giza (III), with a mean of 649.75 bp. In all investigated species, the percentage of GC content found to be nearly 44.01% and the span aligned sequences around 638-663 nucleotides. The number of variable sites within the sequences of the four Moringa genotypes was found to be 46 sites (11 transitions, 15 transversions and 20 indel). A phylogentic tree based on rbcL barcoding was constructed. Three clusters were shown, the first contained Moringa oleifera (R2) and the second contained Moringa oleifera (R1) and Moringa peregrina (R3). While the third cluster contained Moringa peregrina (R4). Several studies have reported similar observations. The results demonstrate that the analysis of these DNA barcode sequences is a reliable method for distinguishing between four Moringa genotypes.
{"title":"Evaluating the efficiency of DNA Barcode rbcL for detection of genetic relationships between four Moringa spp. genotypes","authors":"Walaa A. Rayan","doi":"10.5455/egyjebb.20190722115434","DOIUrl":"https://doi.org/10.5455/egyjebb.20190722115434","url":null,"abstract":"Alterations in the DNA sequences are very useful for the unique markers development, which could be utilized as a barcoding of DNA for different plants. The barcoding of DNA is used for identification of living organisms and provides extra and integral support to identify the morpho-species, because it characterized by a reproducible, rapid and economic tool. This study was conducted to analyze the phylogenetic relationships between two species of the genus Moringa; M. oleifera and M. Peregrina, using DNA barcodes. In Egypt only two Moringa species (M. oleifera and M. Peregrina) were cultivated. These two species (four Moringa genotypes) were commonly used in the traditional medicine. To authenticate the different genotypes, rbcL regions were evaluated. DNA barcode regions were amplified using universal primers. The length of rbcL barcoding region varied between 638 bp in M. oleifera (II) collected from El-Suis governorate (Egypt) to 663 bp in M. peregrina from Giza (III), with a mean of 649.75 bp. In all investigated species, the percentage of GC content found to be nearly 44.01% and the span aligned sequences around 638-663 nucleotides. The number of variable sites within the sequences of the four Moringa genotypes was found to be 46 sites (11 transitions, 15 transversions and 20 indel). A phylogentic tree based on rbcL barcoding was constructed. Three clusters were shown, the first contained Moringa oleifera (R2) and the second contained Moringa oleifera (R1) and Moringa peregrina (R3). While the third cluster contained Moringa peregrina (R4). Several studies have reported similar observations. The results demonstrate that the analysis of these DNA barcode sequences is a reliable method for distinguishing between four Moringa genotypes.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"45 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85432920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.5455/EGYJEBB.20181126081938
Hanan Temerk
{"title":"Isolation and characterization of Haloferax mucsum from Red sea saltern as an halobacterial Archea capable of growing on Naphtaline as an aromatic compounds.","authors":"Hanan Temerk","doi":"10.5455/EGYJEBB.20181126081938","DOIUrl":"https://doi.org/10.5455/EGYJEBB.20181126081938","url":null,"abstract":"","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72707682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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