Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20220528071353
Rabab A Ghanem, R. Hassanein, Saher Elkhawas, A. Younis
The current study evaluated the efficacy of gum arabic (GA) safe edible coating on nutritional properties, phenolic compounds, and antioxidant capacity of grapevine (Vitis vinifera L.) cv. ‘Flame Seedless’. The grapes were coated with 0, 1, 3, 5, 7% GA, and then stored at 4 ± 1°C with 90 ± 2% RH for 30 days. GA coating at 5% significantly reduced weight loss, repressed titratable acidity (TA) degradation, and postponed the rise of total soluble solid (TSS) content. Also, 5% GA treatment suppressed the loss of ascorbic acid (AA), total phenolic content (TPC), as well as individual phenolic compounds. Moreover, 5% GA-coated fruits exhibited substantially the highest antioxidant capacity (DPPH), antioxidant enzyme activities (POD and CAT), and anthocyanin content. Therefore, it is evident that GA at 5% is a potential postharvest coating to maintain overall quality of ‘Flame Seedless’ grapevine fruit during cold storage.
{"title":"The efficacy of gum arabic coating in postharvest preservation of grapes","authors":"Rabab A Ghanem, R. Hassanein, Saher Elkhawas, A. Younis","doi":"10.5455/egyjebb.20220528071353","DOIUrl":"https://doi.org/10.5455/egyjebb.20220528071353","url":null,"abstract":"The current study evaluated the efficacy of gum arabic (GA) safe edible coating on nutritional properties, phenolic compounds, and antioxidant capacity of grapevine (Vitis vinifera L.) cv. ‘Flame Seedless’. The grapes were coated with 0, 1, 3, 5, 7% GA, and then stored at 4 ± 1°C with 90 ± 2% RH for 30 days. GA coating at 5% significantly reduced weight loss, repressed titratable acidity (TA) degradation, and postponed the rise of total soluble solid (TSS) content. Also, 5% GA treatment suppressed the loss of ascorbic acid (AA), total phenolic content (TPC), as well as individual phenolic compounds. Moreover, 5% GA-coated fruits exhibited substantially the highest antioxidant capacity (DPPH), antioxidant enzyme activities (POD and CAT), and anthocyanin content. Therefore, it is evident that GA at 5% is a potential postharvest coating to maintain overall quality of ‘Flame Seedless’ grapevine fruit during cold storage.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78837462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20220109065149
R. Zadeh, M. Koupaei, Sajjad Asgharzadeh, B. Kalani, Johar Pourghader, Atieh Darbandi, V. Mahabadi, F. Jazi
{"title":"Antibacterial activity of medicinal plants and propolis extracts as well as their synergistic effect with antibiotics on pathogenic bacteria","authors":"R. Zadeh, M. Koupaei, Sajjad Asgharzadeh, B. Kalani, Johar Pourghader, Atieh Darbandi, V. Mahabadi, F. Jazi","doi":"10.5455/egyjebb.20220109065149","DOIUrl":"https://doi.org/10.5455/egyjebb.20220109065149","url":null,"abstract":"","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80051043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20221107011617
M. Gharieb, Mai Badawy, G. Elreesh
Oleaginous microorganisms accumulate 20% or more of their dry weight as lipid. In the present study, 247 fungal isolates were obtained from different types of soil, marine water, and food, and screened for their ability to accumulate considerable amounts of lipid. A primary screening with Nile red staining was followed by sulfo-phosphovanillin reaction. The most potent isolate was identified employing both morphological and molecular methods as the Zygomycetous fungus, Cunninghamella echinulata AUMC14396. The lipid of this fungus was extracted, transesterified, and analyzed via GC-MS to elucidate the fatty acid (FA) composition. The fatty acid profile revealed the presence of various fatty acids with different concentrations. Of the total fatty acids, saturated FAs comprised 44.49%, while monounsaturated FAs were 19.91%, and polyunsaturated fatty acids (PUFAs) were 35.61%. The most predominant species was linoleic acid (13.79%) followed by stearic (12.78%) and oleic (12.50%) acids. The polyunsaturated fatty acid, gamma linolenic acid was present in a considerable amount (8.02%). Other important PUFAs were also found in the fatty acid profile such as arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid. The results of this study were discussed in respect to the important applications in the context of production of pharmaceuticals and nutraceuticals.
{"title":"Isolation of oleaginous fungi from natural sources, and fatty acid profile of Cunninghamella echinulata","authors":"M. Gharieb, Mai Badawy, G. Elreesh","doi":"10.5455/egyjebb.20221107011617","DOIUrl":"https://doi.org/10.5455/egyjebb.20221107011617","url":null,"abstract":"Oleaginous microorganisms accumulate 20% or more of their dry weight as lipid. In the present study, 247 fungal isolates were obtained from different types of soil, marine water, and food, and screened for their ability to accumulate considerable amounts of lipid. A primary screening with Nile red staining was followed by sulfo-phosphovanillin reaction. The most potent isolate was identified employing both morphological and molecular methods as the Zygomycetous fungus, Cunninghamella echinulata AUMC14396. The lipid of this fungus was extracted, transesterified, and analyzed via GC-MS to elucidate the fatty acid (FA) composition. The fatty acid profile revealed the presence of various fatty acids with different concentrations. Of the total fatty acids, saturated FAs comprised 44.49%, while monounsaturated FAs were 19.91%, and polyunsaturated fatty acids (PUFAs) were 35.61%. The most predominant species was linoleic acid (13.79%) followed by stearic (12.78%) and oleic (12.50%) acids. The polyunsaturated fatty acid, gamma linolenic acid was present in a considerable amount (8.02%). Other important PUFAs were also found in the fatty acid profile such as arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid. The results of this study were discussed in respect to the important applications in the context of production of pharmaceuticals and nutraceuticals.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"10 4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83405890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20220815041125
Fawzy Younis, M. Mohamed, A. Khan, No author No author, Amira Ramadan, and Ammar
Climate change and related challenges are affecting all over the world, particularly, African's 2030 path of sustainable development, especially in the desert regions, which include the most vulnerable and vulnerable systems. Climate changes are a natural phenomenon that occurs every several millennia, but increased last years as a result of human activity. It transcends the geographical boundaries of countries and poses a global threat. The steady increase in global surface air temperatures has been confirmed (0.3 and 0.6 oc) over the past 100 years. Any disruption of the Earth's ecosystem in general, increasing average atmospheric temperature, melting of the poles, inundation of island States and deltas, disruption of rainfall patterns, negative impacts on agricultural land productivity, water needs, public health and transmission of epidemic diseases. This review light on scientific concept of climate changes, climate change risks, climate worming and human footprint. Moreover, this review aims to discuss the historical development of international agreements, treaties and protocols that discussed addressing climate change and its current and future effects. Moreover, this review aims to discuss the historical development of international agreements, treaties and protocols that discussed addressing climate change and its current and future effects. Briefly, it summarizes what will happen to the blue planet if the reasons of climate changes continue?
{"title":"Climate Changes between International Conventions or Protocols and Scientific Impacts","authors":"Fawzy Younis, M. Mohamed, A. Khan, No author No author, Amira Ramadan, and Ammar","doi":"10.5455/egyjebb.20220815041125","DOIUrl":"https://doi.org/10.5455/egyjebb.20220815041125","url":null,"abstract":"Climate change and related challenges are affecting all over the world, particularly, African's 2030 path of sustainable development, especially in the desert regions, which include the most vulnerable and vulnerable systems. Climate changes are a natural phenomenon that occurs every several millennia, but increased last years as a result of human activity. It transcends the geographical boundaries of countries and poses a global threat. The steady increase in global surface air temperatures has been confirmed (0.3 and 0.6 oc) over the past 100 years. Any disruption of the Earth's ecosystem in general, increasing average atmospheric temperature, melting of the poles, inundation of island States and deltas, disruption of rainfall patterns, negative impacts on agricultural land productivity, water needs, public health and transmission of epidemic diseases. This review light on scientific concept of climate changes, climate change risks, climate worming and human footprint. Moreover, this review aims to discuss the historical development of international agreements, treaties and protocols that discussed addressing climate change and its current and future effects. Moreover, this review aims to discuss the historical development of international agreements, treaties and protocols that discussed addressing climate change and its current and future effects. Briefly, it summarizes what will happen to the blue planet if the reasons of climate changes continue?","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72991324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20220802080606
H. Fathy, Abd Shanshoury, R. Domany, Perihan S. Ateya
Escherichia coli is a crucial indicator of hygiene for animal derived foods and clinical samples. Since E. coli is a zoonotic disease, its contamination poses a severe risk to public health due to the consumption of foods that have been treated with antibiotics and the overuse of antibiotics to treat a variety of bacterial infections in humans. Therefore, the objective of this investigation was to ascertain the emergence of MDR E. coli strains in specific foods and clinical samples from Egypt. Fifteen clinical samples of urine, stool, and pus, and 15 dietary samples containing vegetables, meat, milk, cheese, and chicken were randomly obtained from various locations in Egypt for this study. The thirty E. coli-positive samples that were isolated from the two sources were tested for antibiotic susceptibility to eleven different antibiotics and biochemical identification. According to the study, all clinical isolates were completely resistant to both ampicillin/sulbactam and amoxicillin/clavulanic acid. Cefoxitin and cephalexin resistance were present in 93.3 and 86.6 percent of the isolates, respectively. On the other hand, all clinical isolates were sensitive to Imipenem. However, all the food isolates were sensitive to Levofloxacin, Aztreonam, and Imipenem, whereas 100% of the isolates were resistant to Ampicillin/Sulbactam and 93.3 percent of the isolates were resistant to Amoxicillin/Clavulanic acid. We discovered that both individual and MDR-resistant E. coli were widespread in clinical and feed samples. This may suggest that these foods produced by animals and plants have the potential to contaminate other foods and other peoples with antibiotic-resistant E. coli, raising a potential public health issue. This study highlights the importance of promoting sanitation and minimizing the use of antibiotics. It is crucial to perform a multicenter study to assess the spread and evolution of MDR E. coli in hospital studies, as well as in foods originating from both plants and animals.
{"title":"Prevalence of Escherichia coli and Antibiotic Resistance in Clinical and Food Samples","authors":"H. Fathy, Abd Shanshoury, R. Domany, Perihan S. Ateya","doi":"10.5455/egyjebb.20220802080606","DOIUrl":"https://doi.org/10.5455/egyjebb.20220802080606","url":null,"abstract":"Escherichia coli is a crucial indicator of hygiene for animal derived foods and clinical samples. Since E. coli is a zoonotic disease, its contamination poses a severe risk to public health due to the consumption of foods that have been treated with antibiotics and the overuse of antibiotics to treat a variety of bacterial infections in humans. Therefore, the objective of this investigation was to ascertain the emergence of MDR E. coli strains in specific foods and clinical samples from Egypt. Fifteen clinical samples of urine, stool, and pus, and 15 dietary samples containing vegetables, meat, milk, cheese, and chicken were randomly obtained from various locations in Egypt for this study. The thirty E. coli-positive samples that were isolated from the two sources were tested for antibiotic susceptibility to eleven different antibiotics and biochemical identification. According to the study, all clinical isolates were completely resistant to both ampicillin/sulbactam and amoxicillin/clavulanic acid. Cefoxitin and cephalexin resistance were present in 93.3 and 86.6 percent of the isolates, respectively. On the other hand, all clinical isolates were sensitive to Imipenem. However, all the food isolates were sensitive to Levofloxacin, Aztreonam, and Imipenem, whereas 100% of the isolates were resistant to Ampicillin/Sulbactam and 93.3 percent of the isolates were resistant to Amoxicillin/Clavulanic acid. We discovered that both individual and MDR-resistant E. coli were widespread in clinical and feed samples. This may suggest that these foods produced by animals and plants have the potential to contaminate other foods and other peoples with antibiotic-resistant E. coli, raising a potential public health issue. This study highlights the importance of promoting sanitation and minimizing the use of antibiotics. It is crucial to perform a multicenter study to assess the spread and evolution of MDR E. coli in hospital studies, as well as in foods originating from both plants and animals.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"128 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77554379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20220323094533
Shaimaa Sayed, T. Rahman, Mohesen Sayed, M. Kholy
{"title":"Microbiological Assessement of El-Salam Canal","authors":"Shaimaa Sayed, T. Rahman, Mohesen Sayed, M. Kholy","doi":"10.5455/egyjebb.20220323094533","DOIUrl":"https://doi.org/10.5455/egyjebb.20220323094533","url":null,"abstract":"","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"116 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77273560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20220629084930
M. Bedaiwy, O. Awadalla, M. Metwally, A. Samad
In the present study, twenty-seven fungal species were isolated, purified, and identified from five mixed soil samples collected from Tanta City, Gharbia Governorate, Egypt. The isolated fungal species belonging to nine genera i.e., Aspergillus, Cephalosporium, Fusarium, Penicillium, Botritrichom, Trichoderma, Alternaria, Cladosporium and Scopulariopsis, in addition to two white rot fungi Ganoderma lucidum and Lentinula edodes obtained from Mushroom Laboratory Culture Collection (MLCC), El-Giza, Egypt. The ligninolytic activities of the isolated fungi were evaluated on PDA plates containing 0.02% guaiacol as a chromogenic reagent. Out of the twenty-nine fungal species tested, only three fungi G. lucidum, L. edodes and T. harzianum (isolate 2) showed ligninolytic potential and reddish-brown color zone formed due to guaiacol oxidation. The three positive fungal strains were screened quantitively for Laccase (Lac; E.C. 1.10.3.2), Manganese dependent peroxidase (MnP; E.C. 1.11.1.13), and Lignin peroxidase (LiP; E.C. 1.11.1.14) activities. The highest total ligninase activity was recorded by G. lucidum followed by L. edodes and T. harzianum, respectively.
{"title":"ISOLATION, IDENTIFICATION, AND DETECTION OF LIGNINOLYTIC CAPABILITY OF SOME FUNGI ISOLATED FROM SOIL SAMPLES IN EGYPT.","authors":"M. Bedaiwy, O. Awadalla, M. Metwally, A. Samad","doi":"10.5455/egyjebb.20220629084930","DOIUrl":"https://doi.org/10.5455/egyjebb.20220629084930","url":null,"abstract":"In the present study, twenty-seven fungal species were isolated, purified, and identified from five mixed soil samples collected from Tanta City, Gharbia Governorate, Egypt. The isolated fungal species belonging to nine genera i.e., Aspergillus, Cephalosporium, Fusarium, Penicillium, Botritrichom, Trichoderma, Alternaria, Cladosporium and Scopulariopsis, in addition to two white rot fungi Ganoderma lucidum and Lentinula edodes obtained from Mushroom Laboratory Culture Collection (MLCC), El-Giza, Egypt. The ligninolytic activities of the isolated fungi were evaluated on PDA plates containing 0.02% guaiacol as a chromogenic reagent. Out of the twenty-nine fungal species tested, only three fungi G. lucidum, L. edodes and T. harzianum (isolate 2) showed ligninolytic potential and reddish-brown color zone formed due to guaiacol oxidation. The three positive fungal strains were screened quantitively for Laccase (Lac; E.C. 1.10.3.2), Manganese dependent peroxidase (MnP; E.C. 1.11.1.13), and Lignin peroxidase (LiP; E.C. 1.11.1.14) activities. The highest total ligninase activity was recorded by G. lucidum followed by L. edodes and T. harzianum, respectively.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80077283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20220407080045
Doaa A. Galilah, Omar Shehaby
{"title":"Differential analysis of heavy metals and biochemical compounds of different Mentha species","authors":"Doaa A. Galilah, Omar Shehaby","doi":"10.5455/egyjebb.20220407080045","DOIUrl":"https://doi.org/10.5455/egyjebb.20220407080045","url":null,"abstract":"","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81981347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20220830025307
Amany M. Reyad
The efficacy of lemon oil as a food preservative against the well-known meat pathogen was investigated. Escherichia coli was detected and identified from 20 samples of frozen minced beef by using VITEK II automated system. The disc diffusion method was used to test the antibacterial activity of lemon essential oil diluted in ethanol 70% against E. coli on agar plates. The oil presented a significant antimicrobial activity against the tested bacterium; the minimum inhibitory concentration (MIC) was determined to be 0.25 %, lemon oil (0.25 %) had a measurable inhibitory effect when mixed with beef infected with 106 CFU/ml bacterial load. SEM images of the treated E. coli revealed membrane rupture, abnormal shaped cells, and cellular damage. The volatile profile of the oil revealed that it is mainly composed of monoterpenes, with citral, 6-exohydroxy camphene, and fenchone as the major compounds. The lemon essential oil could be a good alternative for existing biocides due to its potent antibacterial properties and its safety to the environment and human health.
{"title":"Antimicrobial activity of Citrus limon essential oil against Escherichia coli in minced meat","authors":"Amany M. Reyad","doi":"10.5455/egyjebb.20220830025307","DOIUrl":"https://doi.org/10.5455/egyjebb.20220830025307","url":null,"abstract":"The efficacy of lemon oil as a food preservative against the well-known meat pathogen was investigated. Escherichia coli was detected and identified from 20 samples of frozen minced beef by using VITEK II automated system. The disc diffusion method was used to test the antibacterial activity of lemon essential oil diluted in ethanol 70% against E. coli on agar plates. The oil presented a significant antimicrobial activity against the tested bacterium; the minimum inhibitory concentration (MIC) was determined to be 0.25 %, lemon oil (0.25 %) had a measurable inhibitory effect when mixed with beef infected with 106 CFU/ml bacterial load. SEM images of the treated E. coli revealed membrane rupture, abnormal shaped cells, and cellular damage. The volatile profile of the oil revealed that it is mainly composed of monoterpenes, with citral, 6-exohydroxy camphene, and fenchone as the major compounds. The lemon essential oil could be a good alternative for existing biocides due to its potent antibacterial properties and its safety to the environment and human health.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83694040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20220623095227
Oluwakemi Ogunyebi, T. Samuel, A. Osibona, Temitope O. Fadipe
Micromesistius poutassou (Blue whiting) popularly called ‘’Panla’’ in Nigeria. It is one of the affordable fishes imported to Nigeria. With the rising cost of meat, milk and protein foods, consumers have become increasingly interested in fish as a source of protein for its availability and affordability rate. Smoked samples of Micromesistius poutassou were randomly purchased from ten different markets in ten local governments in Lagos state. This collection (purchase) were carried out between June and November 2019 (6 months). The associated fungi were isolated using the pour-plate method and extracted its DNA with the aid of Zymo DNA extraction kit. The extracted fungal DNA were amplified by PCR (Polymerase chain reaction) using specific internal transcribed spacer primer (ITS1/ITS4) and sequenced. Of the total 480 smoked Micromesistius poutassou sampled, ten fungi species were identified and characterised. These fungi include: Kodamaea ohmeri, Daldinia eschscholtzii, Aspergillus longivesica, Aspergillus flavus, Phoma sp, Aspergillus sp, Mucor circinelloides, Aspergillus tamarii, Aspergillus niger, and Geotrichum candidum. Results from this study revealed that smoked Micromesistius poutassou, which are being consumed by Lagosians on the daily basis were heavily contaminated with pathogenic fungi.
{"title":"Molecular Characterization of Fungi Associated with Smoked Micromesistius poutassou (Blue whiting) from Different Markets in Lagos, Nigeria.","authors":"Oluwakemi Ogunyebi, T. Samuel, A. Osibona, Temitope O. Fadipe","doi":"10.5455/egyjebb.20220623095227","DOIUrl":"https://doi.org/10.5455/egyjebb.20220623095227","url":null,"abstract":"Micromesistius poutassou (Blue whiting) popularly called ‘’Panla’’ in Nigeria. It is one of the affordable fishes imported to Nigeria. With the rising cost of meat, milk and protein foods, consumers have become increasingly interested in fish as a source of protein for its availability and affordability rate. Smoked samples of Micromesistius poutassou were randomly purchased from ten different markets in ten local governments in Lagos state. This collection (purchase) were carried out between June and November 2019 (6 months). The associated fungi were isolated using the pour-plate method and extracted its DNA with the aid of Zymo DNA extraction kit. The extracted fungal DNA were amplified by PCR (Polymerase chain reaction) using specific internal transcribed spacer primer (ITS1/ITS4) and sequenced. Of the total 480 smoked Micromesistius poutassou sampled, ten fungi species were identified and characterised. These fungi include: Kodamaea ohmeri, Daldinia eschscholtzii, Aspergillus longivesica, Aspergillus flavus, Phoma sp, Aspergillus sp, Mucor circinelloides, Aspergillus tamarii, Aspergillus niger, and Geotrichum candidum. Results from this study revealed that smoked Micromesistius poutassou, which are being consumed by Lagosians on the daily basis were heavily contaminated with pathogenic fungi.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90521502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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