Pub Date : 2023-01-01DOI: 10.5455/egyjebb.20230513103233
Ethar A. Hussein, E. Kamel, Nagwan Hamdy
Acacia s.l. taxa in the Middle East are treated as a single genus and suffer from confusion in its discrimination because of morphological similarities between closely related taxa. So, the current study aims at revealing distinguishing characters, and assessing the taxonomic relationships between studied taxa using recently proposed genera versus Acacia s.l. genus in conventional classificatory systems. Macro-, micro-morphological characters, and SDS-protein pattern of 13 Acacia s.l. spp. were investigated focusing on seeds using the scanning electron microscope (SEM). The generated UPGMA-cluster based on 106 morphological and biochemical character-states split the studied Acacia s.l. taxa into two groups; (1) comprised genera of Vachellia, Acacia s.s. and Faidherbia, and (II) Senegalia genus. The present results gave accurate discrimination of genus Acacia s.l., confirmed the need of its retypification in the Middle East according to the new system of classification and show the close relation between Vachellia, each of Acacia s.s. and Faidherbia genera.
{"title":"Taxonomic implications of morphological variations and seed protein profile in Acacia Mill. (Fabaceae, Mimosoideae)","authors":"Ethar A. Hussein, E. Kamel, Nagwan Hamdy","doi":"10.5455/egyjebb.20230513103233","DOIUrl":"https://doi.org/10.5455/egyjebb.20230513103233","url":null,"abstract":"Acacia s.l. taxa in the Middle East are treated as a single genus and suffer from confusion in its discrimination because of morphological similarities between closely related taxa. So, the current study aims at revealing distinguishing characters, and assessing the taxonomic relationships between studied taxa using recently proposed genera versus Acacia s.l. genus in conventional classificatory systems. Macro-, micro-morphological characters, and SDS-protein pattern of 13 Acacia s.l. spp. were investigated focusing on seeds using the scanning electron microscope (SEM). The generated UPGMA-cluster based on 106 morphological and biochemical character-states split the studied Acacia s.l. taxa into two groups; (1) comprised genera of Vachellia, Acacia s.s. and Faidherbia, and (II) Senegalia genus. The present results gave accurate discrimination of genus Acacia s.l., confirmed the need of its retypification in the Middle East according to the new system of classification and show the close relation between Vachellia, each of Acacia s.s. and Faidherbia genera.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87240864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5455/egyjebb.20221207072545
R. Zadeh, M. Zadeh, Mohammad Sholeh, Sajjad Asgharzadeh, Atieh Darbandi, Parisa Fashami, N. Amirmozafari
One of the most common bacteria in nosocomial and burn infections is Pseudomonas aeruginosa. Due to the high antibiotic resistance of P. aeruginosa and the lack of other effective therapeutic agents, treating diseases caused by this bacterium could be challenging. Therefore, finding novel solutions to control and prevent highly resistant strains of P. aeruginosa is paramount. After isolation and identification of Lactobacilli with known probiotic properties such as L. acidophilus, L. paracasei, L. rhamnosus, and L. bulgaricus using PCR technique, and preparation of their corresponding phage (PAO1 Φ), probiotics and phage were used individually and in combination to treat burn infection caused by P. aeruginosa in a mouse model. The results revealed that the combination of phage with probiotics can be highly effective for treating P. aeruginosa burn infections. Our study showed that the most effective multiple cocktails of Lactobacilli for the treatment of P. aeruginosa infection, were L. rhamnosus + L. bulgaricus + L. paracasei cocktail in mixture with phage (PAO1 Φ). The study proposes an alternative method for the treatment of P. aeruginosa infections. The combination of probiotics with phage could be proposed as a new therapeutic agent for treating P. aeruginosa infections.
{"title":"Bacteriophage and probiotic therapy in the treatment of Pseudomonas aeruginosa burn infection and their synergistic effect","authors":"R. Zadeh, M. Zadeh, Mohammad Sholeh, Sajjad Asgharzadeh, Atieh Darbandi, Parisa Fashami, N. Amirmozafari","doi":"10.5455/egyjebb.20221207072545","DOIUrl":"https://doi.org/10.5455/egyjebb.20221207072545","url":null,"abstract":"One of the most common bacteria in nosocomial and burn infections is Pseudomonas aeruginosa. Due to the high antibiotic resistance of P. aeruginosa and the lack of other effective therapeutic agents, treating diseases caused by this bacterium could be challenging. Therefore, finding novel solutions to control and prevent highly resistant strains of P. aeruginosa is paramount. After isolation and identification of Lactobacilli with known probiotic properties such as L. acidophilus, L. paracasei, L. rhamnosus, and L. bulgaricus using PCR technique, and preparation of their corresponding phage (PAO1 Φ), probiotics and phage were used individually and in combination to treat burn infection caused by P. aeruginosa in a mouse model. The results revealed that the combination of phage with probiotics can be highly effective for treating P. aeruginosa burn infections. Our study showed that the most effective multiple cocktails of Lactobacilli for the treatment of P. aeruginosa infection, were L. rhamnosus + L. bulgaricus + L. paracasei cocktail in mixture with phage (PAO1 Φ). The study proposes an alternative method for the treatment of P. aeruginosa infections. The combination of probiotics with phage could be proposed as a new therapeutic agent for treating P. aeruginosa infections.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88149041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20220726105555
Naglaa Elshafey, A. Elkashef, H. Hamedo, M. Mansour
Solar salterns are an ideal model for extreme environments since they contain a halophilic microorganisms that must adapt to these conditions. In North Sinai, these environments have not gotten much attention. However, the focus of our investigation was on the salterns' waters and saline soil. The study of this microflora showed that it had a lot of different shapes, functions, and ways of using energy. The molecular analysis provided us with a phylogenetic relationship that included Aspergillus isolates as well as archaeal isolates from the genera Haloarcula, Haloferex, and Halobacterium, and also bacteria related to Halomonas and Brevibacillus. The majority of isolates expressed hydrolytic enzymes at high salt concentrations. Finally, the North Sinai Solar Saltern is rich in halophilic populations that produce industrial hydrolytic enzymes.
{"title":"Molecular identification of Halophilic Microorganisms producing Exo-Halozymes by a culture-dependent approach from the North Sinai Solar saltern","authors":"Naglaa Elshafey, A. Elkashef, H. Hamedo, M. Mansour","doi":"10.5455/egyjebb.20220726105555","DOIUrl":"https://doi.org/10.5455/egyjebb.20220726105555","url":null,"abstract":"Solar salterns are an ideal model for extreme environments since they contain a halophilic microorganisms that must adapt to these conditions. In North Sinai, these environments have not gotten much attention. However, the focus of our investigation was on the salterns' waters and saline soil. The study of this microflora showed that it had a lot of different shapes, functions, and ways of using energy. The molecular analysis provided us with a phylogenetic relationship that included Aspergillus isolates as well as archaeal isolates from the genera Haloarcula, Haloferex, and Halobacterium, and also bacteria related to Halomonas and Brevibacillus. The majority of isolates expressed hydrolytic enzymes at high salt concentrations. Finally, the North Sinai Solar Saltern is rich in halophilic populations that produce industrial hydrolytic enzymes.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75855148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20220729121826
E. Elsherbeny
The Boraginaceae family comprises around 2000 species within 145 genera with various therapeutic and pharmacological attributes. DNA banking can be considered a complementary method for preserving plant species by preserving genomic DNA at lower temperatures. This approach to maintaining biological information allows researchers to search for new genes and their products. Therefore, in this review, we describe and analyze the DNA barcoding of family Boraginaceae to conserve and further manipulate this rare and endangered plants species to enhance its yield and quality characteristics. The non-coding DNA regions (such as ITS and rbcL) are presumed to be more useful at lower taxonomic levels because of their faster evolutionary rates. The present study aims to inspect whether these plants can be categorized into different species or not, and DNA barcoding is used to clarify the dispute. The DNA barcode marker used ITS and rbcL, and sequence analysis was performed using the online tool BLAST of the NCBI database and based on maximum identity and dendrogram.
{"title":"Taxonomical and genetics characterization of family Boraginaceae grown in Elba Mountain","authors":"E. Elsherbeny","doi":"10.5455/egyjebb.20220729121826","DOIUrl":"https://doi.org/10.5455/egyjebb.20220729121826","url":null,"abstract":"The Boraginaceae family comprises around 2000 species within 145 genera with various therapeutic and pharmacological attributes. DNA banking can be considered a complementary method for preserving plant species by preserving genomic DNA at lower temperatures. This approach to maintaining biological information allows researchers to search for new genes and their products. Therefore, in this review, we describe and analyze the DNA barcoding of family Boraginaceae to conserve and further manipulate this rare and endangered plants species to enhance its yield and quality characteristics. The non-coding DNA regions (such as ITS and rbcL) are presumed to be more useful at lower taxonomic levels because of their faster evolutionary rates. The present study aims to inspect whether these plants can be categorized into different species or not, and DNA barcoding is used to clarify the dispute. The DNA barcode marker used ITS and rbcL, and sequence analysis was performed using the online tool BLAST of the NCBI database and based on maximum identity and dendrogram.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76636441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20220407081622
O. Elshehaby, Doaa A. Galilah
{"title":"Assessment of some physiological responses of Carica papaya L. against lead treatment","authors":"O. Elshehaby, Doaa A. Galilah","doi":"10.5455/egyjebb.20220407081622","DOIUrl":"https://doi.org/10.5455/egyjebb.20220407081622","url":null,"abstract":"","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82417205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20220120025332
G. Allah, A. Mahalawy, Iman Kholy, Samar S. Mohamed
Invasive fungal infections (IFIs) have emerged in the last three decades as an important cause of human disease. These infections treated particularly with triazole such as fluconazole, itraconazole and voriconazole but some serious adverse reactions have been reported in many studies . In vitro activities of 12 essential oils were evaluated against 6 clinically important fungal strains. Thyme oil was the most effective essential oil against all the tested fungal strains and Aspergillus flavus was the most susceptible with inhibition zone diameter and MIC values of 39 mm and 0.25 μg/ml, respectively. Active ingredients of thyme oil were analysed using GC/Mass chromatography and extracted by Thin Layer Chromatography (TLC). Thymol was the most active component against Aspergillus flavus with inhibition zone diameter and MIC values of 44 mm and 0.125 μg/ml, respectively. The inhibitory activity of thymol against Aspergillus flavus was confirmed using Transmission electron microscope.
侵袭性真菌感染(IFIs)在过去三十年中作为人类疾病的一个重要原因出现。这些感染主要用氟康唑、伊曲康唑和伏立康唑等三唑治疗，但许多研究报告了一些严重的不良反应。研究了12种精油对6种临床重要真菌的体外活性。百里香精油对真菌的抑菌效果最好，对黄曲霉的抑菌效果最好，抑菌带直径为39 mm, MIC值为0.25 μg/ml。采用气相色谱/质层色谱法对百里香精油的有效成分进行分析，并采用薄层色谱法对百里香精油进行提取。百里香酚对黄曲霉的抑制作用最强，抑制带直径为44 mm, MIC值为0.125 μg/ml。透射电镜法证实了百里香酚对黄曲霉的抑制作用。
{"title":"Antifungal Activity of Thymol Against Some Medically Important Fungi","authors":"G. Allah, A. Mahalawy, Iman Kholy, Samar S. Mohamed","doi":"10.5455/egyjebb.20220120025332","DOIUrl":"https://doi.org/10.5455/egyjebb.20220120025332","url":null,"abstract":"Invasive fungal infections (IFIs) have emerged in the last three decades as an important cause of human disease. These infections treated particularly with triazole such as fluconazole, itraconazole and voriconazole but some serious adverse reactions have been reported in many studies . In vitro activities of 12 essential oils were evaluated against 6 clinically important fungal strains. Thyme oil was the most effective essential oil against all the tested fungal strains and Aspergillus flavus was the most susceptible with inhibition zone diameter and MIC values of 39 mm and 0.25 μg/ml, respectively. Active ingredients of thyme oil were analysed using GC/Mass chromatography and extracted by Thin Layer Chromatography (TLC). Thymol was the most active component against Aspergillus flavus with inhibition zone diameter and MIC values of 44 mm and 0.125 μg/ml, respectively. The inhibitory activity of thymol against Aspergillus flavus was confirmed using Transmission electron microscope.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87539525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20220317090305
Marwa M Ragaey, N. Hussein, A. Khatib, M. Ismail, M. Dawood
{"title":"Impact of phosphate solubilizing fungi on soil properties and physiological parameters of pepper plants","authors":"Marwa M Ragaey, N. Hussein, A. Khatib, M. Ismail, M. Dawood","doi":"10.5455/egyjebb.20220317090305","DOIUrl":"https://doi.org/10.5455/egyjebb.20220317090305","url":null,"abstract":"","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77177214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20220823083832
Marwa Nakeep, Basma Khalaf, Samha Dowidar, A. Mohsen, M. Elhaak
The widespread availability of using nano technology can ensure food security through boosting agriculture production. Nanoparticles have potential to improve growth and yield of wheat crop. This paper studies effects of wheat grains priming in nanoparticles and bulk calcium hydroxide on germination, growth criteria, photosynthetic pigments, carbohydrates, and proteins content of wheat. Calcium hydroxide nanoparticles were 73nm size. Wheat grains were soaked for four hours in 0, 8.0, and 16.0 mM nanoparticles and 16 mM bulk solutions of calcium hydroxide for germination experiments in the laboratory. Also soaked grains were cultivated in clay-sandy soil (2:1 w/w), in small plastic bags in the green house of faculty of science for studying growth and some metabolic activities. Results showed that application of calcium hydroxide nanoparticles increased germination percentage significantly with 8 and 16 mM nanoparticles in comparison to control and calcium hydroxide bulk. Grain germination was hundred percentages with the 16.0 mM nanoparticles concentration. Root and shoot fresh weight and dry weight increased significantly (about 30%) at 8 and 16 mM as compared to control and calcium hydroxide bulk. Higher concentration (16 mM) of calcium hydroxide nanoparticles significantly enhanced fresh weight, dry weight, chlorophyll and protein content over control and calcium hydroxide bulk. Nonetheless, effect of 8 and 16mM caused more utilization of carbohydrates in the plant growth as compared to control. Calcium hydroxide nanoparticles have definite potential to increase crop growth and yield. Nonetheless, comprehensive experimentation is required to establish the most suitable concentration, size and mode of application of calcium hydroxide nanoparticles for higher growth and yield in the field crops.
{"title":"Utilization of nano calcium for improving wheat germination, growth, and metabolic activities","authors":"Marwa Nakeep, Basma Khalaf, Samha Dowidar, A. Mohsen, M. Elhaak","doi":"10.5455/egyjebb.20220823083832","DOIUrl":"https://doi.org/10.5455/egyjebb.20220823083832","url":null,"abstract":"The widespread availability of using nano technology can ensure food security through boosting agriculture production. Nanoparticles have potential to improve growth and yield of wheat crop. This paper studies effects of wheat grains priming in nanoparticles and bulk calcium hydroxide on germination, growth criteria, photosynthetic pigments, carbohydrates, and proteins content of wheat. Calcium hydroxide nanoparticles were 73nm size. Wheat grains were soaked for four hours in 0, 8.0, and 16.0 mM nanoparticles and 16 mM bulk solutions of calcium hydroxide for germination experiments in the laboratory. Also soaked grains were cultivated in clay-sandy soil (2:1 w/w), in small plastic bags in the green house of faculty of science for studying growth and some metabolic activities. Results showed that application of calcium hydroxide nanoparticles increased germination percentage significantly with 8 and 16 mM nanoparticles in comparison to control and calcium hydroxide bulk. Grain germination was hundred percentages with the 16.0 mM nanoparticles concentration. Root and shoot fresh weight and dry weight increased significantly (about 30%) at 8 and 16 mM as compared to control and calcium hydroxide bulk. Higher concentration (16 mM) of calcium hydroxide nanoparticles significantly enhanced fresh weight, dry weight, chlorophyll and protein content over control and calcium hydroxide bulk. Nonetheless, effect of 8 and 16mM caused more utilization of carbohydrates in the plant growth as compared to control. Calcium hydroxide nanoparticles have definite potential to increase crop growth and yield. Nonetheless, comprehensive experimentation is required to establish the most suitable concentration, size and mode of application of calcium hydroxide nanoparticles for higher growth and yield in the field crops.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86476269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20220327060847
Mohamed Esawi, Enas Ballat, A. Elsayed
{"title":"Exogenous application of neem extract enhances the control of bacterial spot of tomato grown in soilless culture system through modulating gas exchange attributes, osmolytes, antioxidant machinery and genes expression","authors":"Mohamed Esawi, Enas Ballat, A. Elsayed","doi":"10.5455/egyjebb.20220327060847","DOIUrl":"https://doi.org/10.5455/egyjebb.20220327060847","url":null,"abstract":"","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86786389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20220727012311
M. Elhaak, M. Ahmed, M. Zayed, Dina Gad
The study aims to investigate effect of soaking Ammi visnaga seeds in the growth hormones gibberellic acid, kinetin, and naphthalene acetic acid (GA3, KN and NAA). Growth parameters at vegetative stage, in addition to the secondary compounds content especially khellin were measured using the HPLC analysis. Results showed that soaking in GA3 led to a noticeable increase in the length of the plant shoot, while the root depth increased by treatment with the NAA. Soaking seeds with GA3 100 ppm caused the highest plant weight with an increase by 63.38% compared with the control. High content of Chl. b and Carotenoids was observed by KN, while of Chl. it was by NAA. Soaking by the three plant hormones (GA3, KN and NAA) caused a slight increase in the content of the important medicinal component (khellin) in the plant seeds which suggested their utilization for increasing the medicinal value of Ammi visnaga.
{"title":"The impacts of gibberellic acid, kinetin, and naphthalene acetic acid on Ammi visnaga important medicinal compound (khellin)","authors":"M. Elhaak, M. Ahmed, M. Zayed, Dina Gad","doi":"10.5455/egyjebb.20220727012311","DOIUrl":"https://doi.org/10.5455/egyjebb.20220727012311","url":null,"abstract":"The study aims to investigate effect of soaking Ammi visnaga seeds in the growth hormones gibberellic acid, kinetin, and naphthalene acetic acid (GA3, KN and NAA). Growth parameters at vegetative stage, in addition to the secondary compounds content especially khellin were measured using the HPLC analysis. Results showed that soaking in GA3 led to a noticeable increase in the length of the plant shoot, while the root depth increased by treatment with the NAA. Soaking seeds with GA3 100 ppm caused the highest plant weight with an increase by 63.38% compared with the control. High content of Chl. b and Carotenoids was observed by KN, while of Chl. it was by NAA. Soaking by the three plant hormones (GA3, KN and NAA) caused a slight increase in the content of the important medicinal component (khellin) in the plant seeds which suggested their utilization for increasing the medicinal value of Ammi visnaga.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"90 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84364318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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