Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20211229081234
S. Teleb, Hussein J. Hussein, Rajaa Mabrouk
: Thirteen species of the Solanaceae from Egypt were examined for their anther characteristics and the pollen morphology using LM and SEM. The investigated species representing nine genera viz. Atropa , Cestrum , Datura, Hyoscyamus, Nicotiana, Petunia, Physalis, Solanum, and Withania. The exertion of anthers from the corolla tube, the mode of anther attachment with the filament, and the ways of anthers dehiscence, as well as the anthers colour and shape, represent diagnostic variation among the species. The sculpturing patterns viewed on the surface of the anther were potentially useful in the identification of many of the species. The pollen grains were isopolar, tricolporate, suboblate, subprolate to prolate, and with notable differences in the exine sculpturing. The computational analysis of 34 scored character–states affirmed the usefulness of such characters in clarifying the taxonomic relationships among the examined species.
{"title":"Taxonomic Significance of Anthers and Pollen grains of Some Species of Solanaceae from Egypt","authors":"S. Teleb, Hussein J. Hussein, Rajaa Mabrouk","doi":"10.5455/egyjebb.20211229081234","DOIUrl":"https://doi.org/10.5455/egyjebb.20211229081234","url":null,"abstract":": Thirteen species of the Solanaceae from Egypt were examined for their anther characteristics and the pollen morphology using LM and SEM. The investigated species representing nine genera viz. Atropa , Cestrum , Datura, Hyoscyamus, Nicotiana, Petunia, Physalis, Solanum, and Withania. The exertion of anthers from the corolla tube, the mode of anther attachment with the filament, and the ways of anthers dehiscence, as well as the anthers colour and shape, represent diagnostic variation among the species. The sculpturing patterns viewed on the surface of the anther were potentially useful in the identification of many of the species. The pollen grains were isopolar, tricolporate, suboblate, subprolate to prolate, and with notable differences in the exine sculpturing. The computational analysis of 34 scored character–states affirmed the usefulness of such characters in clarifying the taxonomic relationships among the examined species.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87913133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20220216032804
Ali Ali, Iman Kholy, A. Ismaiel, Mohammed A. Mohammed, A. Din, Y. Shetaia
{"title":"A Study of Fungal Infection Prevalence, Risk Factors, Molecular Identification and In Vitro Susceptibility Testing In Patients with Pulmonary Mycosis","authors":"Ali Ali, Iman Kholy, A. Ismaiel, Mohammed A. Mohammed, A. Din, Y. Shetaia","doi":"10.5455/egyjebb.20220216032804","DOIUrl":"https://doi.org/10.5455/egyjebb.20220216032804","url":null,"abstract":"","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"102 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78173486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20220724091455
M. Rizk, A. Hassan, M. Zayat
Abstract To satisfy the needs of a growing global population, it is vital to develop viable replacement crops. Moringa is a tropical and subtropical plant that grows in multiple regions in the world. The Moringaceae family includes the genus M. peregrina, it has been discovered to have a variety of medical purposes; therefore M. peregrina must be grown in a variety of locations around Egypt. The purpose of this study is to determine the RNA sequences of tow genes and their phylogenetic relationships of 2780 sequence alignments collected from NCBI as well as M. peregrina from 255 genera across the novel gene cyt C, and 1955 sequence alignments as well as M. peregrina from 177 genera accessions numbers in NCBI (MZ005963 and MZ005966, respectively). Furthermore, cyt C (879 bp) whilst, cyt b6f complex (468bp). The present study included the investigation of allele frequency, partial haploid, haploid SNPs, SNPs transitions, and Transversions M. peregrina. The phylogenetic analysis was utilized to evaluate M. peregrina genetic variation and association with other plant species, according to our findings. Using sequence alignment from NCBI as well as Moringa, we were able to identify multiple cytochrome genes according to sequence alignment. The sequence alignment of the cytochrome C and cytochrome b6f complex genes reveals a high level of genetic variety and diversity among Moringa and other species of plants.
摘要为了满足日益增长的全球人口的需求，开发可行的替代作物是至关重要的。辣木是一种热带和亚热带植物，生长在世界多个地区。辣木科包括M. peregrina属，它已被发现具有多种医学用途;因此，佩雷格里纳必须在埃及各地的各种地方种植。本研究的目的是通过NCBI中255个属的cyt C和177个属的NCBI(分别为MZ005963和MZ005966)的2780个序列比对和1955个序列比对，确定两个基因的RNA序列及其系统发育关系。此外，cyt C (879 bp)和cyt b6f复合物(468bp)。本研究包括等位基因频率、部分单倍体、单倍体单核苷酸多态性、单核苷酸多态性转移和转位的调查。利用系统发育分析方法评价了该植物的遗传变异及其与其他植物的关联。利用NCBI和辣木的序列比对，我们能够根据序列比对鉴定出多个细胞色素基因。细胞色素C和细胞色素b6f复合物基因的序列比对表明，辣木和其他植物具有高度的遗传多样性和多样性。
{"title":"Structure function of the Cytochrome C and Cytochrome b6f complex genes in Moringa peregrina","authors":"M. Rizk, A. Hassan, M. Zayat","doi":"10.5455/egyjebb.20220724091455","DOIUrl":"https://doi.org/10.5455/egyjebb.20220724091455","url":null,"abstract":"Abstract To satisfy the needs of a growing global population, it is vital to develop viable replacement crops. Moringa is a tropical and subtropical plant that grows in multiple regions in the world. The Moringaceae family includes the genus M. peregrina, it has been discovered to have a variety of medical purposes; therefore M. peregrina must be grown in a variety of locations around Egypt. The purpose of this study is to determine the RNA sequences of tow genes and their phylogenetic relationships of 2780 sequence alignments collected from NCBI as well as M. peregrina from 255 genera across the novel gene cyt C, and 1955 sequence alignments as well as M. peregrina from 177 genera accessions numbers in NCBI (MZ005963 and MZ005966, respectively). Furthermore, cyt C (879 bp) whilst, cyt b6f complex (468bp). The present study included the investigation of allele frequency, partial haploid, haploid SNPs, SNPs transitions, and Transversions M. peregrina. The phylogenetic analysis was utilized to evaluate M. peregrina genetic variation and association with other plant species, according to our findings. Using sequence alignment from NCBI as well as Moringa, we were able to identify multiple cytochrome genes according to sequence alignment. The sequence alignment of the cytochrome C and cytochrome b6f complex genes reveals a high level of genetic variety and diversity among Moringa and other species of plants.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82244925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20211222055050
Kholoud Ali, H. Gebreel, H. Youssef
{"title":"The potential role of probiotics to adhere with caco-2 cells and control the antibiotic-resistant foodborne pathogens adhesion in vitro","authors":"Kholoud Ali, H. Gebreel, H. Youssef","doi":"10.5455/egyjebb.20211222055050","DOIUrl":"https://doi.org/10.5455/egyjebb.20211222055050","url":null,"abstract":"","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75281968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20220110015832
El Mohammed, Azza Demerdash, Riham A. Ibrahim, M. Hazaa, R. Elghaffar, Amira E Sehim
{"title":"Prevalence and Multidrug Resistance Pattern of beta-lactam Resistant Streptococcus pyogenes Isolated from Nasopharyngeal Infections","authors":"El Mohammed, Azza Demerdash, Riham A. Ibrahim, M. Hazaa, R. Elghaffar, Amira E Sehim","doi":"10.5455/egyjebb.20220110015832","DOIUrl":"https://doi.org/10.5455/egyjebb.20220110015832","url":null,"abstract":"","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"197 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81078047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.5455/egyjebb.20220326070207
Mohamed Esawi, Enas Ballat, A. Elsayed
{"title":"Defensin treatment induces resistance to bacterial wilt of soilless-cultured tomato by regulating antioxidant mechanisms and genes expression","authors":"Mohamed Esawi, Enas Ballat, A. Elsayed","doi":"10.5455/egyjebb.20220326070207","DOIUrl":"https://doi.org/10.5455/egyjebb.20220326070207","url":null,"abstract":"","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88965577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5455/egyjebb.15-1624733139
A. Haider, Salma Abdeldayem, M. El-Esawi
Brassica campestris could be used for medicinal purposes. The plant is rich in flavonoids and phenolics that have potential for pharmacological purposes. As such, enhancement of secondary metabolites in Brassica campestris plants is highly recommended. Therefore, the aim of this study is to obtain cultures of hairy roots of Brassica campestris, which has undergone a bacterial transformation using Agrobacterium rhizogenes in an attempt to enhance the production of secondary metabolites. The level of expression of root inducing gene in transformed hairy roots was estimated using quantitative real-time PCR analysis. Results revealed higher root inducing gene expression levels in the transgenic plants as compared to the nontransformed roots. Transformed hairy roots showed higher increases in the total phenolic content, as compared to non-transformed roots. This study indicated the enhanced secondary metabolites biosynthesis by hairy roots induction in Brassica campestris plants.
{"title":"Induction of Hairy Roots and Phenolics Biosynthesis of Genetically-Transformed Brassica campestris L","authors":"A. Haider, Salma Abdeldayem, M. El-Esawi","doi":"10.5455/egyjebb.15-1624733139","DOIUrl":"https://doi.org/10.5455/egyjebb.15-1624733139","url":null,"abstract":"Brassica campestris could be used for medicinal purposes. The plant is rich in flavonoids and phenolics that have potential for pharmacological purposes. As such, enhancement of secondary metabolites in Brassica campestris plants is highly recommended. Therefore, the aim of this study is to obtain cultures of hairy roots of Brassica campestris, which has undergone a bacterial transformation using Agrobacterium rhizogenes in an attempt to enhance the production of secondary metabolites. The level of expression of root inducing gene in transformed hairy roots was estimated using quantitative real-time PCR analysis. Results revealed higher root inducing gene expression levels in the transgenic plants as compared to the nontransformed roots. Transformed hairy roots showed higher increases in the total phenolic content, as compared to non-transformed roots. This study indicated the enhanced secondary metabolites biosynthesis by hairy roots induction in Brassica campestris plants.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"258 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76372426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5455/egyjebb.20210430085804
E. Elsherbeny, S. Hassanen, M. Diab
Aerva Lanata (L.) Juss. ex Schult is an important medicinal multipurpose plant that belongs to the family Amaranthaceae. An efficient protocol for In vitro propagation of A. Lanata was established. Nodal segment explants collected from mature shrubs from the Gebel Elba, South Egypt. Explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of 6-benzyl adenine (BA) (2.22, 4.44, 8.90 and 17.80 M) and Kinetin (Kin) (2.32, 4.60, 9.30, and 18.6M) individually for shoot induction. The highest shoot induction percentage (93.33%) with maximum number for shoots (4.07 shoots per explant) was noticed on MS medium supplemented with 4.44 M BA. The maximum number of multiple shoots per explants (9.13) was obtained in the combination BA (4.44 M) and NAA (2.14 M). For root induction, the highest rooting percentage (80%) with mean root number (4.33 roots per shoot) and length (3.83 cm) of roots were noticed on half- strength MS medium augmented with 4.90 M IBA. Rooted plantlets were transferred into plastic pots containing sand, peat moss and garden soil mixture at equal volumes, and successfully acclimatized in the greenhouse with a survival rate of 73.3%. Plant identification is a crucial aspect to understand and conserve plant diversity from extinction. DNA barcode analysis of A. Lanata was carried out using. The present in vitro propagation protocol would facilitate an alternative method for rapid and large-scal production of this important medicinal plant. We therefore, used the rbcL gene to identify A. lanata. Maximum likelihood tree analysis was performed to evaluate the discriminatory power of the rbcL gene. Our findings showed that using rbcL gene sequences enabled identifying the majority of the samples (90%) to the genus and species level.
{"title":"In vitro propagation and DNA barcoding of the rare medicinal plant Aerva Lanata (L.) Juss. ex Schult grown in Gebel Elba","authors":"E. Elsherbeny, S. Hassanen, M. Diab","doi":"10.5455/egyjebb.20210430085804","DOIUrl":"https://doi.org/10.5455/egyjebb.20210430085804","url":null,"abstract":"Aerva Lanata (L.) Juss. ex Schult is an important medicinal multipurpose plant that belongs to the family Amaranthaceae. An efficient protocol for In vitro propagation of A. Lanata was established. Nodal segment explants collected from mature shrubs from the Gebel Elba, South Egypt. Explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of 6-benzyl adenine (BA) (2.22, 4.44, 8.90 and 17.80 M) and Kinetin (Kin) (2.32, 4.60, 9.30, and 18.6M) individually for shoot induction. The highest shoot induction percentage (93.33%) with maximum number for shoots (4.07 shoots per explant) was noticed on MS medium supplemented with 4.44 M BA. The maximum number of multiple shoots per explants (9.13) was obtained in the combination BA (4.44 M) and NAA (2.14 M). For root induction, the highest rooting percentage (80%) with mean root number (4.33 roots per shoot) and length (3.83 cm) of roots were noticed on half- strength MS medium augmented with 4.90 M IBA. Rooted plantlets were transferred into plastic pots containing sand, peat moss and garden soil mixture at equal volumes, and successfully acclimatized in the greenhouse with a survival rate of 73.3%. Plant identification is a crucial aspect to understand and conserve plant diversity from extinction. DNA barcode analysis of A. Lanata was carried out using. The present in vitro propagation protocol would facilitate an alternative method for rapid and large-scal production of this important medicinal plant. We therefore, used the rbcL gene to identify A. lanata. Maximum likelihood tree analysis was performed to evaluate the discriminatory power of the rbcL gene. Our findings showed that using rbcL gene sequences enabled identifying the majority of the samples (90%) to the genus and species level.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77952460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5455/egyjebb.20211021042802
E. Ammar, Nihal ELghandour, Moustafa S. Mouhamed, Nehal E. Elkaliny
: This review aims to focusing on different agricultural biotechnologies that seek to enhance the crop productivity. Biotechnology refers to any technological application makes or modifies products or processes for specific usage using biological systems. Agricultural biotechnology is a group of scientific methods for improving productivity of plants, animals or their defence to microbes. Scientists have developed strategies to increase agricultural productivity based on understanding of DNA incorporation recombinant technology into traditional breeding, agricultural research. The targeted introduction of desired genes into the crop could speed up breeding. Main goal of transgenic breeding is to improve agronomic and qualitative features of crops. Genetic engineering refers to the deliberate altering of the genome of an organism by the introduction of one or few particular foreign genes, while the modified organism refers to a transformed (transgenic) organism. improving crops which breeding speed and efficiency depending on directly or indirectly modifying genes and genomic areas that underpin essential features. Overexpression, models of the of
{"title":"Crop productivity enhancement; Agricultural biotechnology viewpoint","authors":"E. Ammar, Nihal ELghandour, Moustafa S. Mouhamed, Nehal E. Elkaliny","doi":"10.5455/egyjebb.20211021042802","DOIUrl":"https://doi.org/10.5455/egyjebb.20211021042802","url":null,"abstract":": This review aims to focusing on different agricultural biotechnologies that seek to enhance the crop productivity. Biotechnology refers to any technological application makes or modifies products or processes for specific usage using biological systems. Agricultural biotechnology is a group of scientific methods for improving productivity of plants, animals or their defence to microbes. Scientists have developed strategies to increase agricultural productivity based on understanding of DNA incorporation recombinant technology into traditional breeding, agricultural research. The targeted introduction of desired genes into the crop could speed up breeding. Main goal of transgenic breeding is to improve agronomic and qualitative features of crops. Genetic engineering refers to the deliberate altering of the genome of an organism by the introduction of one or few particular foreign genes, while the modified organism refers to a transformed (transgenic) organism. improving crops which breeding speed and efficiency depending on directly or indirectly modifying genes and genomic areas that underpin essential features. Overexpression, models of the of","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88886990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5455/egyjebb.20211118082536
H. Shora, M. Metwally, Mohammed Zaki
{"title":"Optimization of L-glutaminase production by Penicillium chrysogenum","authors":"H. Shora, M. Metwally, Mohammed Zaki","doi":"10.5455/egyjebb.20211118082536","DOIUrl":"https://doi.org/10.5455/egyjebb.20211118082536","url":null,"abstract":"","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"111 3S 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80802981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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