Pub Date : 2019-01-01DOI: 10.5455/egyjebb.20190709123823
A. Zeid, Eman E. Zaher, K. Allah, R. Ahmed
Depletion of fossil fuel is the largest challenge facing human industrial and technological revolution in the current era. Exploration of widely distributed microorganisms for the production of renewable biofuel is the most priority for scientific research in the last decades. In this context, thirteen fungal isolates were collected from Tanta city, El-Gharbia Governorate, Egypt belonging to four genera (Aspergillus, Penicillium, Fusarium and Alternaria) were screened on minimal media for their ability to accumulate lipid in their biomass. The maximum lipid contents were 25.31% for Aspergillus versicolor and 20.15% for Aspergillus niveus. The optimization result showed that pH 4, incubation temperature 32°C, sucrose at concentration of 40g/L, glutamic acid at concentration of 0.5 g/L (C/N ratio at 40:0.5 w/w) after 5 days of incubation with agitation rate of 150 rpm on orbital shaker were the most appropriate conditions for the highest lipid accumulation in the case of Aspergillus versicolor. However, in case of Aspergillus niveus, pH 4, incubation temperature at 32°C, sucrose at concentration of 20 g/L, NaNO3 at concentration of 1.0 g/L (C/N ratio at 20:1.0 w/w) after 11 days of incubation with agitation rate of 150 rpm were the most appropriate for the highest lipid accumulation.
{"title":"Screening and optimization of some Egyptian soil-born fungi for lipids production as possible source for biofuel","authors":"A. Zeid, Eman E. Zaher, K. Allah, R. Ahmed","doi":"10.5455/egyjebb.20190709123823","DOIUrl":"https://doi.org/10.5455/egyjebb.20190709123823","url":null,"abstract":"Depletion of fossil fuel is the largest challenge facing human industrial and technological revolution in the current era. Exploration of widely distributed microorganisms for the production of renewable biofuel is the most priority for scientific research in the last decades. In this context, thirteen fungal isolates were collected from Tanta city, El-Gharbia Governorate, Egypt belonging to four genera (Aspergillus, Penicillium, Fusarium and Alternaria) were screened on minimal media for their ability to accumulate lipid in their biomass. The maximum lipid contents were 25.31% for Aspergillus versicolor and 20.15% for Aspergillus niveus. The optimization result showed that pH 4, incubation temperature 32°C, sucrose at concentration of 40g/L, glutamic acid at concentration of 0.5 g/L (C/N ratio at 40:0.5 w/w) after 5 days of incubation with agitation rate of 150 rpm on orbital shaker were the most appropriate conditions for the highest lipid accumulation in the case of Aspergillus versicolor. However, in case of Aspergillus niveus, pH 4, incubation temperature at 32°C, sucrose at concentration of 20 g/L, NaNO3 at concentration of 1.0 g/L (C/N ratio at 20:1.0 w/w) after 11 days of incubation with agitation rate of 150 rpm were the most appropriate for the highest lipid accumulation.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87254320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5455/EGYJEBB.20181122122145
Sabha Ahmed Al-Sabbagh, O. Eissa, M. Sallam
Different endopthytic fungi isolated from Catheranthus roseus were tested for their ability to produce Taxol. Taxol extracted from fermentation culture of the isolated fungus Cladosporium cladosporioides was identified by UV, TLC, mass spectroscopy and HPLC. The isolated strain was identified as Cladosporium cladosporioides based on morphological characteristics and the analysis of the 18S rDNA sequence. HPLC quantification of fungal Taxol showed that Cladosporium cladosporioides was capable of producing 700µg/l of Taxol on potato dextrose broth. The optimum culture conditions for Taxol production by the isolated Cladosporium cladosporioides were tested on M1D medium after 10 days of incubation at 25 °C, pH 5.5 in the presence of sucrose and ammonium tartrate as carbon and nitrogen sources respectively. Also different concentrations of benzoic acid, 4-hydroxy benzoic acid, vanadyl sulphate, serinol and salicylic acid were evaluated for their inducing activity on Taxol production by the isolated endophytic fungus. This study offers important information and a new source for the production of the important anticancer drug Taxol by endophytic fungus fermentation. The antitumor activity of fungal Taxol was tested against human tumor cell line HepG-2 (liver carcinoma) using in vitro clonogenic assays.
{"title":"Taxol production by an endophytic fungus, Cladosporium and #8206; cladosporioides isolated from Catheranthus roseus","authors":"Sabha Ahmed Al-Sabbagh, O. Eissa, M. Sallam","doi":"10.5455/EGYJEBB.20181122122145","DOIUrl":"https://doi.org/10.5455/EGYJEBB.20181122122145","url":null,"abstract":"Different endopthytic fungi isolated from Catheranthus roseus were tested for their ability to produce Taxol. Taxol extracted from fermentation culture of the isolated fungus Cladosporium cladosporioides was identified by UV, TLC, mass spectroscopy and HPLC. The isolated strain was identified as Cladosporium cladosporioides based on morphological characteristics and the analysis of the 18S rDNA sequence. HPLC quantification of fungal Taxol showed that Cladosporium cladosporioides was capable of producing 700µg/l of Taxol on potato dextrose broth. The optimum culture conditions for Taxol production by the isolated Cladosporium cladosporioides were tested on M1D medium after 10 days of incubation at 25 °C, pH 5.5 in the presence of sucrose and ammonium tartrate as carbon and nitrogen sources respectively. Also different concentrations of benzoic acid, 4-hydroxy benzoic acid, vanadyl sulphate, serinol and salicylic acid were evaluated for their inducing activity on Taxol production by the isolated endophytic fungus. This study offers important information and a new source for the production of the important anticancer drug Taxol by endophytic fungus fermentation. The antitumor activity of fungal Taxol was tested against human tumor cell line HepG-2 (liver carcinoma) using in vitro clonogenic assays.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75090750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5455/EGYJEBB.20190628044001
S. Darier, Eman T El Kenany, Esraa Fawzy
A simple mathematical model was applied to assess the magnitude of contributions of allelospoly (competition on resources and energy) and allelopathy (secretion of biochemical) to interference against Raphanus sativus by Medicago sativa. The seedling growth of R. sativus (plumule and radicle lengths) are significantly (P ≤ 0.05) affected by allelochemicals extracted from M. sativa. Competitive interference by M. sativa was eliminated and there were high coefficient of determination (R²) values in dry weight of shoot and root of R. sativus. In other experiment, an equivalent amount of allelopathic effect to that involved in the interference was added, not eliminated. Split-plot designs were used with three replicates. The main-plot factors were three mixtures and the sub-plot factors were: without interference, with interference, and with interference plus allelopathic effects. The reduction in shoot and root dry weight of R. sativus in mixed culture treated with the extract was mainly ascribed to allelopathy. The results suggested that the complex effect of both components of interference (allelopathy and allelospoly) on the protein profile of the recipient species, and confirms the modeling results. Meanwhile, allelospoly exhibited only a physical effect which can be evaluated through the growth parameters.
{"title":"Assessment of contribution of allelospoly and allelopathy to interference of Medicago sativa with Raphanus sativus","authors":"S. Darier, Eman T El Kenany, Esraa Fawzy","doi":"10.5455/EGYJEBB.20190628044001","DOIUrl":"https://doi.org/10.5455/EGYJEBB.20190628044001","url":null,"abstract":"A simple mathematical model was applied to assess the magnitude of contributions of allelospoly (competition on resources and energy) and allelopathy (secretion of biochemical) to interference against Raphanus sativus by Medicago sativa. The seedling growth of R. sativus (plumule and radicle lengths) are significantly (P ≤ 0.05) affected by allelochemicals extracted from M. sativa. Competitive interference by M. sativa was eliminated and there were high coefficient of determination (R²) values in dry weight of shoot and root of R. sativus. In other experiment, an equivalent amount of allelopathic effect to that involved in the interference was added, not eliminated. Split-plot designs were used with three replicates. The main-plot factors were three mixtures and the sub-plot factors were: without interference, with interference, and with interference plus allelopathic effects. The reduction in shoot and root dry weight of R. sativus in mixed culture treated with the extract was mainly ascribed to allelopathy. The results suggested that the complex effect of both components of interference (allelopathy and allelospoly) on the protein profile of the recipient species, and confirms the modeling results. Meanwhile, allelospoly exhibited only a physical effect which can be evaluated through the growth parameters.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82897967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5455/egyjebb.20191130124643
M. Nour
{"title":"Functional properties and medical benefits of pomegranate (Punica granatum L.) peels as agro-industrial wastes","authors":"M. Nour","doi":"10.5455/egyjebb.20191130124643","DOIUrl":"https://doi.org/10.5455/egyjebb.20191130124643","url":null,"abstract":"","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76313545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5455/egyjebb.20190619110050
Mohamed Y. Hazman, M. Sameh, Menna Mahmoud Abd Elzaher, N. Mohamed, Nada Essam El din Diab, N. Mikhael, Nora Ashraf Hussin, Nourhan A. Helmy, S.E.N. Mohamed
Egypt, the largest rice producer in the near east and North Africa, reached the point where the insufficient irrigation water (drought) and its low quality (saline) imposed serious limitations on rice production. Understanding molecular adaptive mechanisms through studying the expression profile of wide range of different stress-marker genes can consolidate breeding programs aimed to develop certain rice varieties capable of fitting in challenging agro-ecosystems. Rice is notoriously sensitive to drought and salinity but there is variation within examined rice varieties represented in two genetically unlike Egyptian rice commercial cultivars: Sakha 106 (Japonica) and Giza 179 (Indica/Japonica). The two Egyptian rice genotypes were submitted to osmotically equivalent doses of drought (mimicked by mannitol) and/or salinity (triggered by NaCl). The observed significant alternations in the transcriptional response among 14 stress-related genes displayed a genotype dependent and stress specific pattern. The expected knowledge might enhance the background about how rice plants differ in sensing and restoring its adaptive mechanisms in response to drought and salinity, two kind of stresses that frequently co-occur in the Egyptian agricultural environment. KEYWORDS: Rice, drought, salinity, japonica, indica, stress-marker genes, quantitative PCR
{"title":"Drought and Salinity Alter Adaptive Molecular Response in Two Genetically unlike Egyptian Rice Cultivars","authors":"Mohamed Y. Hazman, M. Sameh, Menna Mahmoud Abd Elzaher, N. Mohamed, Nada Essam El din Diab, N. Mikhael, Nora Ashraf Hussin, Nourhan A. Helmy, S.E.N. Mohamed","doi":"10.5455/egyjebb.20190619110050","DOIUrl":"https://doi.org/10.5455/egyjebb.20190619110050","url":null,"abstract":"Egypt, the largest rice producer in the near east and North Africa, reached the point where the insufficient irrigation water (drought) and its low quality (saline) imposed serious limitations on rice production. Understanding molecular adaptive mechanisms through studying the expression profile of wide range of different stress-marker genes can consolidate breeding programs aimed to develop certain rice varieties capable of fitting in challenging agro-ecosystems. Rice is notoriously sensitive to drought and salinity but there is variation within examined rice varieties represented in two genetically unlike Egyptian rice commercial cultivars: Sakha 106 (Japonica) and Giza 179 (Indica/Japonica). The two Egyptian rice genotypes were submitted to osmotically equivalent doses of drought (mimicked by mannitol) and/or salinity (triggered by NaCl). The observed significant alternations in the transcriptional response among 14 stress-related genes displayed a genotype dependent and stress specific pattern. The expected knowledge might enhance the background about how rice plants differ in sensing and restoring its adaptive mechanisms in response to drought and salinity, two kind of stresses that frequently co-occur in the Egyptian agricultural environment. KEYWORDS: Rice, drought, salinity, japonica, indica, stress-marker genes, quantitative PCR","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78578535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5455/egyjebb.20190804010102
Ghada Shebl, H. S. Ahmed, A. Kato, H. Dawoud, M. Hamza, A. Haider
Phenylketonuria (PKU) is a relatively common autosomal recessive disorder. The frequency of PKU among Caucasians populations is approximately 1:10.000. PKU is primarily a consequence of a deficiency in phenylalanine hydroxylase (PAH) activity, and un-treated PKU patients develop severe mental retardation. The absence of this enzymatic activity causes disturbances in tyrosine and tryptophan metabolism and diminished formation of catecholamine (dopamine), melanin and serotonin. In the present study, nucleotide sequence mutations were examined and detected in Egyptian phenylketonuria (PKU) patients. Total genomic DNA was extracted from the blood of six PKU patients and specific PAH primers were used to amplify genomic DNA fragments. Nucleotide sequences were processed using Human Splicing Finder software to remove introns and predict cDNAs. Processed cDNA sequences were aligned with reference sequence in the GenBank and healthy control for detection of mutations. The results indicated different sequence polymorphism, which can cause mild or severe mutations. The severity of sequence mutation was correlated with the accumulation of phenylalanine amino acid in the blood. This study is an attempt to early detection of PAH mutations, to control phenylketonuria genetic disorder in Egyptian population.
{"title":"Detection of sequence mutations in phenylalanine hydroxylase (PAH) gene isolated from Egyptian Phenylketonuria (PKU) patients","authors":"Ghada Shebl, H. S. Ahmed, A. Kato, H. Dawoud, M. Hamza, A. Haider","doi":"10.5455/egyjebb.20190804010102","DOIUrl":"https://doi.org/10.5455/egyjebb.20190804010102","url":null,"abstract":"Phenylketonuria (PKU) is a relatively common autosomal recessive disorder. The frequency of PKU among Caucasians populations is approximately 1:10.000. PKU is primarily a consequence of a deficiency in phenylalanine hydroxylase (PAH) activity, and un-treated PKU patients develop severe mental retardation. The absence of this enzymatic activity causes disturbances in tyrosine and tryptophan metabolism and diminished formation of catecholamine (dopamine), melanin and serotonin. In the present study, nucleotide sequence mutations were examined and detected in Egyptian phenylketonuria (PKU) patients. Total genomic DNA was extracted from the blood of six PKU patients and specific PAH primers were used to amplify genomic DNA fragments. Nucleotide sequences were processed using Human Splicing Finder software to remove introns and predict cDNAs. Processed cDNA sequences were aligned with reference sequence in the GenBank and healthy control for detection of mutations. The results indicated different sequence polymorphism, which can cause mild or severe mutations. The severity of sequence mutation was correlated with the accumulation of phenylalanine amino acid in the blood. This study is an attempt to early detection of PAH mutations, to control phenylketonuria genetic disorder in Egyptian population.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77593655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5455/egyjebb.20190707112923
E. Attia
Medicinal plants are considered a bountiful origin of antimicrobial compounds. Punica granatum commonly known as pomegranate has developed as a medicinal plant with a possibility of antimicrobial activity. So, this study aimed to First:-Extract the antimicrobial components from Pomegranate peel by 95% ethanol and then determine their in vitro effects against some clinical pathogenic bacteria and fungi. The clinical isolates were obtained from the National Research Center in Dokki, and Intensive Care Units (Ismailia), Suez Canal University Hospital. Second: - Study the chemical composition of the peel extract by GC MS chromatogram (Phytochemical analysis). By using the diffusion agar method, Punica granatum ethanolic extract used with different concentrations (10, 20, 30,40) mg/well against the studied pathogenic bacteria and fungi. These different concentrations were highly active against pathogenic bacteria like Staphylococcus aureus ATCC29213 and Klebsiella pneumonia ATCC13883. However, not active against the pathogenic fungi Aspergillus niger, Candida albicans ATCC10231, and Candida pelliculosa MH248066. Different concentrations of Punica granatum ethanolic extract (200,400,600.800) mg/well gave marked inhibition against all the tested fungal species. The zone of inhibition was compared with different standard antimicrobial agents as Streptomycin and Rifampin for bacteria, Amphotericin B and fluconazole for fungi as a positive control. Forty bioactive phytochemical compounds were identified in the Ethanolic extract of Punica Granatum by GC-MS method based on the peak area, molecular weight and retention time. Among forty compounds identified, only fourteen were reported to have biological activities. Our results indicate that Punica granatum contains various bioactive components
{"title":"Antimicrobial Activity and Bio-active compounds analysis in Ethanolic plant extracts of Punica Grantanum (Pomegranate) using GC-MS","authors":"E. Attia","doi":"10.5455/egyjebb.20190707112923","DOIUrl":"https://doi.org/10.5455/egyjebb.20190707112923","url":null,"abstract":"Medicinal plants are considered a bountiful origin of antimicrobial compounds. Punica granatum commonly known as pomegranate has developed as a medicinal plant with a possibility of antimicrobial activity. So, this study aimed to First:-Extract the antimicrobial components from Pomegranate peel by 95% ethanol and then determine their in vitro effects against some clinical pathogenic bacteria and fungi. The clinical isolates were obtained from the National Research Center in Dokki, and Intensive Care Units (Ismailia), Suez Canal University Hospital. Second: - Study the chemical composition of the peel extract by GC MS chromatogram (Phytochemical analysis). By using the diffusion agar method, Punica granatum ethanolic extract used with different concentrations (10, 20, 30,40) mg/well against the studied pathogenic bacteria and fungi. These different concentrations were highly active against pathogenic bacteria like Staphylococcus aureus ATCC29213 and Klebsiella pneumonia ATCC13883. However, not active against the pathogenic fungi Aspergillus niger, Candida albicans ATCC10231, and Candida pelliculosa MH248066. Different concentrations of Punica granatum ethanolic extract (200,400,600.800) mg/well gave marked inhibition against all the tested fungal species. The zone of inhibition was compared with different standard antimicrobial agents as Streptomycin and Rifampin for bacteria, Amphotericin B and fluconazole for fungi as a positive control. Forty bioactive phytochemical compounds were identified in the Ethanolic extract of Punica Granatum by GC-MS method based on the peak area, molecular weight and retention time. Among forty compounds identified, only fourteen were reported to have biological activities. Our results indicate that Punica granatum contains various bioactive components","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80023184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5455/egyjebb.20190804100423
Fatma Elzahraa Taofik Zaky, E. Shabana, Hanaa Sayed Shalaby
Recently Biodiesel has a lot of concerns by number of researchers , due to its environmental benefits, a renewable energy resource, could replace fossil fuels, and the fact that lack of fossil fuels has become imminent. The cost of biodiesel production is the main barrier. The objectives of the present study was economic mass production of microalgae growing on dairy waste (sweet whey ) for lipid accumulation. The obtained data revealed that diluted waste (50%), when compared with (BBM) medium was a promising alternative medium for cultivating of microalgae Anabaena oryzae, Chlorella vulgaris and mixture of both. While the difference in (chlorophyll a and dry weight) obtained from 50 % waste and BBM cultures of all tested organisms was insignificant, the lipid percentage increased by (29.3,13.4 and 16.5 % ) in Anabaena, Chlorella and their mixture respectively, compared with corresponding control cultures. Maximum lipid productivity of Anabaena, Chlorella and their mixture was (2.03, 5.78 and 4.88 mg/l/day). Role of microalgae in biological treatment of waste was noticeable, whereas reduction in all tested parameters (TSS, TDS, COD, TN, NH3, NO3 and P) was recorded. Extracted oil was esterified and analyzed using GC-Ms analysis. The predicted biodiesel properties using obtained fatty acid profile promised with good clean biodiesel meet international qualities of biodiesel
{"title":"Feasibility of microalgae culturing on Dairy waste for biodiesel production","authors":"Fatma Elzahraa Taofik Zaky, E. Shabana, Hanaa Sayed Shalaby","doi":"10.5455/egyjebb.20190804100423","DOIUrl":"https://doi.org/10.5455/egyjebb.20190804100423","url":null,"abstract":"Recently Biodiesel has a lot of concerns by number of researchers , due to its environmental benefits, a renewable energy resource, could replace fossil fuels, and the fact that lack of fossil fuels has become imminent. The cost of biodiesel production is the main barrier. The objectives of the present study was economic mass production of microalgae growing on dairy waste (sweet whey ) for lipid accumulation. The obtained data revealed that diluted waste (50%), when compared with (BBM) medium was a promising alternative medium for cultivating of microalgae Anabaena oryzae, Chlorella vulgaris and mixture of both. While the difference in (chlorophyll a and dry weight) obtained from 50 % waste and BBM cultures of all tested organisms was insignificant, the lipid percentage increased by (29.3,13.4 and 16.5 % ) in Anabaena, Chlorella and their mixture respectively, compared with corresponding control cultures. Maximum lipid productivity of Anabaena, Chlorella and their mixture was (2.03, 5.78 and 4.88 mg/l/day). Role of microalgae in biological treatment of waste was noticeable, whereas reduction in all tested parameters (TSS, TDS, COD, TN, NH3, NO3 and P) was recorded. Extracted oil was esterified and analyzed using GC-Ms analysis. The predicted biodiesel properties using obtained fatty acid profile promised with good clean biodiesel meet international qualities of biodiesel","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83960532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5455/EGYJEBB.20190602120328
Neveen Sadik, U. Hameed, Salma Ghany, M. Ibrahim, M. Tantawy
Araceae is a large and ancient monocot family most notable for its impressive morphological diversity. The current work included the investigation of morphological criteria of some taxa of Araceae viz. whole plant, leaf architecture, stomatography (LM & SEM) leaf, petiole and stem in addition to molecular criteria (RAPD) to throw light on the taxonomic importance of these characters. The amalgamated character states Viz. morphological (269 attributes) and molecular (149 bands) of total (418 attributes) of the studied taxa were phenetically analyzed by aid of NTSys-pc software (version 2.02). The generated phenogram expressed the similarities/dissimilarities among the present taxa. The interrelationships at specific level were discussed and aligned with some of the classification systems. The target of the coducted study is trying to explore the phenetic inter-specific relations among the taxa under study through the sharing of morphological and molecular characters in addition to the assessment of such characters. The combined morphological and molecular criteria segregated Alocasia odora away from the remainings and grouped Philodendron erubescens, Scindapsus aureus and Spathiphyllum walisii in distinct group while the remaining six in another distinct one.
{"title":"Morphological and molecular phenetics on some taxa of family Araceae","authors":"Neveen Sadik, U. Hameed, Salma Ghany, M. Ibrahim, M. Tantawy","doi":"10.5455/EGYJEBB.20190602120328","DOIUrl":"https://doi.org/10.5455/EGYJEBB.20190602120328","url":null,"abstract":"Araceae is a large and ancient monocot family most notable for its impressive morphological diversity. The current work included the investigation of morphological criteria of some taxa of Araceae viz. whole plant, leaf architecture, stomatography (LM & SEM) leaf, petiole and stem in addition to molecular criteria (RAPD) to throw light on the taxonomic importance of these characters. The amalgamated character states Viz. morphological (269 attributes) and molecular (149 bands) of total (418 attributes) of the studied taxa were phenetically analyzed by aid of NTSys-pc software (version 2.02). The generated phenogram expressed the similarities/dissimilarities among the present taxa. The interrelationships at specific level were discussed and aligned with some of the classification systems. The target of the coducted study is trying to explore the phenetic inter-specific relations among the taxa under study through the sharing of morphological and molecular characters in addition to the assessment of such characters. The combined morphological and molecular criteria segregated Alocasia odora away from the remainings and grouped Philodendron erubescens, Scindapsus aureus and Spathiphyllum walisii in distinct group while the remaining six in another distinct one.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88149846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5455/egyjebb.20190407015126
A. Farag, A. Mohammed, M. Afifi, Usama M. Abdel Raouf
Eighteen bacterial strains were isolated from Sea water collected from different sites of Red Sea, Egypt. They were screened for antagonistic activity against many bacterial pathogens. The most active antimicrobial producer (R6) which isolated from Safaga middle was identified as Bacillus badius strain AAUM using 16S rRNA phylogenetic analysis. The most control variables were selected from Plackett–Burman (PB) factorial design for the production of antimicrobial agent from Bacillus badius strain AAUM. It achieved 1.56-fold fold increase (against S. aureus) when grown in medium composed of g/l: peptone, 2.5; yeast extract, 5.0; sea water, 25ml; size of inoculum (3ml/50ml), adjusted to pH 9 and incubation period 20 hrs at 45oC. Immobilization using entrapment and adsorption techniques were applied to improve the productivity of cells. B. badius strain AAUM adsorbed on polyurethane foam realized 1.4-fold increase than the free cells. Reused of the adsorbed culture caused an increase of antimicrobial agent production by 1.14-fold than the free cells. The predicted crude antimicrobial spectra suggested that the B. badius strain AAUM can produce important and novel antimicrobial drugs.
{"title":"Isolation, optimization and production of antimicrobial agent from a novel producer Bacillus badius strain AAUM","authors":"A. Farag, A. Mohammed, M. Afifi, Usama M. Abdel Raouf","doi":"10.5455/egyjebb.20190407015126","DOIUrl":"https://doi.org/10.5455/egyjebb.20190407015126","url":null,"abstract":"Eighteen bacterial strains were isolated from Sea water collected from different sites of Red Sea, Egypt. They were screened for antagonistic activity against many bacterial pathogens. The most active antimicrobial producer (R6) which isolated from Safaga middle was identified as Bacillus badius strain AAUM using 16S rRNA phylogenetic analysis. The most control variables were selected from Plackett–Burman (PB) factorial design for the production of antimicrobial agent from Bacillus badius strain AAUM. It achieved 1.56-fold fold increase (against S. aureus) when grown in medium composed of g/l: peptone, 2.5; yeast extract, 5.0; sea water, 25ml; size of inoculum (3ml/50ml), adjusted to pH 9 and incubation period 20 hrs at 45oC. Immobilization using entrapment and adsorption techniques were applied to improve the productivity of cells. B. badius strain AAUM adsorbed on polyurethane foam realized 1.4-fold increase than the free cells. Reused of the adsorbed culture caused an increase of antimicrobial agent production by 1.14-fold than the free cells. The predicted crude antimicrobial spectra suggested that the B. badius strain AAUM can produce important and novel antimicrobial drugs.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89737115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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