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Identification of chromosomes involved in translocations in wild Emmer 野生Emmer易位相关染色体的鉴定
Pub Date : 1994-01-01 DOI: 10.1266/JJG.69.371
K. Nishikawa, S. Mizuno, Y. Furuta
With the aid of telocentric lines of Emmer wheat, the chromosomes involved in seven chromosome types (one standard type and six translocation types) in wild Emmer, Triticum dicoccoides, were identified. Type Ela was of almost the same chromosome structure as that of durum LD 222 with a small reciprocal translocation between chromosomes 4B and 2B. Type Elb had a major translocation between 2A and 2B and a minor one probably between 2B and 3B. Type E2 had a major translocation between 2B and 3B. Type E3 had a major translocation between 5B and 7B. Type E4 had a major translocation between 4B and 3B and a minor one between 2B and 4B or 3B. Type E5 had a major translocation between 6B and 7B. Type E6 had a major translocation between 1A and 5A. We discussed the result in comparison with the previously reported data on the same translocations.
利用二粒小麦远心系,对野生二粒小麦Triticum dicoccoides的7种染色体类型(1种标准型和6种易位型)进行了染色体鉴定。Ela型与durum LD 222的染色体结构基本相同,但在4B和2B染色体之间存在少量的反向易位。Elb型主要易位在2A和2B之间,次要易位可能在2B和3B之间。E2型主要在2B和3B之间易位。E3型在5B和7B之间有主要的易位。E4型主要易位于4B和3B之间,次要易位于2B和4B或3B之间。E5型在6B和7B之间有主要的易位。E6型主要易位在1A和5A之间。我们将这一结果与先前报道的相同易位的数据进行了比较。
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引用次数: 7
Sectoring patterns of spontaneous and radiation-induced somatic pink mutations in the stamen hairs of a temperature-sensitive mutable clone of Tradescantia 一个温度敏感变异无性系雄蕊自发和辐射诱导体细胞粉红色突变的分割模式
Pub Date : 1994-01-01 DOI: 10.1266/JJG.69.577
S. Ichikawa
The sectoring patterns of somatic pink mutations were analyzed in the stamen hairs of Tradescantia clone KU 20, a temperature-sensitive mutable clone. This clone is a blue/pink heterozygote, and its spontaneous pink mutation frequency increases up to about 40-fold at lower temperature. In order to elucidate the mutable nature of this clone, the sectoring patterns were analyzed on 1,123 spontaneous pink mutant events and on 2,725 pink mutant events induced by 0.606 and 1.28 Gy of gamma rays. The average number of pink cells per terminal pink mutant event (a row of pink cells including the terminal cell of a hair) occurred spontaneously was 7.40, whereas the number for the terminal pink mutant event induced by gamma rays varied from 3.33 to 9.88 depending on the post-irradiation days, i.e. , increased gradually as the number of days proceeded, then was stabilized at the level of spontaneous mutations after about three weeks. The average number of pink cells per interstitial pink mutant event (a single pink cell or two or more contiguous pink cells between blue cells) was 1.97 for spontaneous mutations, while the number for induced mutations varied also depending on the post-irradiation days. The ratio of the number of interstitial pink mutant events against that of terminal pink mutant events was 1.35 for spontaneous mutations, but the ratio for induced mutations varied also with post-irradiation period reaching 2.89 at the peak, indicating that more interstitial pink mutant events are induced by gamma rays than terminal pink mutant events, as compared with spontaneous mutations. The frequency of multiple pink mutant sectors in a hair was more than four times higher than that expected from independent occurrences in case of spontaneous mutations, while the frequency was close to the expectation in induced mutations, suggesting that somatic recombination is involved as one of the major causes of spontaneous mutations in this mutable clone.
研究了温度敏感型变克隆川花(Tradescantia) KU 20雄蕊体细胞粉色突变的分选模式。该无性系为蓝/粉杂合子,低温下其粉红自发突变频率可达40倍左右。为了阐明该克隆的易变性,对1,123个自发粉色突变体和2,725个受0.606和1.28 Gy γ射线诱导的粉色突变体进行了切分模式分析。每个末端粉红突变事件(包括一根头发的末端细胞在内的一排粉红色细胞)自发发生的粉红细胞数平均为7.40个,而伽马射线诱导的末端粉红突变事件的数量根据照射后天数的不同在3.33 ~ 9.88个之间变化,即随着照射天数的增加而逐渐增加,约三周后稳定在自发突变水平。自发突变时,每个间质粉红色突变事件(单个粉红色细胞或蓝色细胞之间的两个或多个相邻粉红色细胞)的平均粉红色细胞数为1.97,而诱导突变的数量也取决于辐照后的天数。自发突变间质粉色突变与末端粉色突变的比值为1.35,而诱导突变的比值也随辐照后时间的变化而变化,峰值为2.89,说明与自发突变相比,伽马射线诱导的间质粉色突变事件比末端粉色突变事件更多。一根头发中多个粉色突变区出现的频率比自发突变中独立出现的频率高出4倍以上,而在诱导突变中出现的频率接近预期,这表明体细胞重组是该可变克隆中自发突变的主要原因之一。
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引用次数: 10
Physical mapping of chloroplast DNAs of Agropyron glaucum, Ag. trichophorum and Haynaldia villosa using alloplasmic common wheat with cytoplasms of respective species Agropyron glaucum, Ag叶绿体dna的物理定位。毛缕菌和海纳尔菌利用同种异质普通小麦与各自品种的细胞质
Pub Date : 1994-01-01 DOI: 10.1266/JJG.69.645
C. Nakamura, K. Ohtani, N. Mori, I. Panayotov, C. Kaneda
Chloroplast DNAs (cpDNAs) of Agropyron glaucum, Ag. trichophorum and Haynaldia villosa were studied using alloplasmic hybrids of common wheat (Triticum aestivum; nuclear donor) with cytoplasms of the respective species. Chloroplast genome sizes of the two Agropyron species and Hy. villosa were very close to 135 kb of common wheat. Restriction maps were constructed using seven restriction enzymes. Eight fragment length mutations (deletions/insertions) and five recognition site mutations were detected among 167 sites studied. CpDNAs of Agropyron, Hy. villosa and common wheat were very closely related: Base substitution rate per 100 base pairs (d) was 0.10 between Hy. villosa and common wheat, 0.19 between common wheat and Agropyron, and 0.29 between Agropyron and Hy. villosa. There was only one site difference (Hindlll site) between the two Agropyron species (d=0.05).
Agropyron glaucum, Ag.的叶绿体dna (cpdna)。以普通小麦(Triticum aestivum;核供体)具有各自物种的细胞质。两种小麦的叶绿体基因组大小与普通小麦的135kb非常接近。用7种限制性内切酶构建了限制性内切图谱。167个位点中检测到8个片段长度突变(缺失/插入)和5个识别位点突变。Agropyron、Hy. villosa和普通小麦的cpdna亲缘关系非常密切:每100个碱基对中,Hy. villosa与普通小麦的碱基取代率(d)为0.10,普通小麦与Agropyron的碱基取代率为0.19,Agropyron与Hy. villosa的碱基取代率为0.29。两种稻草间仅有1个位点(hindll位点)差异(d=0.05)。
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引用次数: 0
Varietal variations in biochemical changes during growth and redifferentiation of rice callus cultures 水稻愈伤组织生长和再分化过程中生化变化的品种差异
Pub Date : 1994-01-01 DOI: 10.1266/JJG.69.385
T. Abe, Y. Oka, T. Sasahara
Calli initiated from 7 rice varieties belonging to 3 subspecies, indica, japonica, and javanica were cultured for 2 and 14 months on the callus maintenance medium, transferred to the plant regeneration medium, and various organ redifferentiation/plant regeneration abilities were examined. Considerable variations especially in the shoot regeneration were observed among the varieties; four varieties [Tadukan (indica), Nipponbare and Sasanishiki (japonica) and Allorio (javanica)] retained the same redifferentiation abilities, while the remaining 3 varieties [Fujisaka 5 (japonica) and Te-tep and Choukoutou (indica)] showed only very much lowered abilities after 14 months. During the redifferentiation/regeneration period, samples were taken at 4-day intervals up to 36 days and peroxidase and acid phosphatase activities were measured. Activities of these enzymes increased after transfer to the regeneration medium. The patterns of the increase, although the same among varieties within the same subspecies, differed markedly between subspecies. Significant correlations between the redifferentiation/regeneration abilities and the activities of the two enzymes were observed.
以籼稻、粳稻和javanica 3个亚种的7个水稻品种愈伤组织为材料,在愈伤组织维持培养基上培养2个月和14个月,然后转移到植株再生培养基上,观察其器官再分化和植株再生能力。不同品种间的茎部再生差异较大;4个品种[多根(籼稻)、日本光和佐三石(粳稻)和Allorio(爪牙)]保持了相同的再分化能力,而其余3个品种[藤坂5号(粳稻)和特特步和秋口(籼稻)]在14个月后的再分化能力仅下降了很多。在再分化/再生期间,每隔4天至36天取样一次,测定过氧化物酶和酸性磷酸酶的活性。转移到再生培养基后,这些酶的活性增加。虽然同一亚种内的不同品种之间的增长模式相同,但亚种之间的增长模式差异显著。再分化/再生能力与这两种酶的活性之间存在显著的相关性。
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引用次数: 8
Structural changes of rye chromosome 1R induced by a gametocidal chromosome 杀配子体染色体诱导黑麦染色体1R的结构变化
Pub Date : 1994-01-01 DOI: 10.1266/JJG.69.13
T. Endo, Maki Yamamoto, Y. Mukai
A certain chromosome of Aegilops triuncialis is known to cause chromosomal aberrations in common wheat. We studied how frequently the Ae. triuncialis chromosome induced chromosome mutations in chromosome 1R of rye which was substituted for chromosome 1B in a common wheat cultivar. Wheat-rye translocations and deletions in chromosome 1R were found in more than 10% of the plants examined, and most of them were stably transmitted in the subsequent generations. The possible use of this system for inducing wheat-alien translocations is discussed in relation to wheat breeding.
已知在普通小麦中,三叉棘的某条染色体会引起染色体畸变。我们研究了Ae。三松毛虫染色体诱导黑麦1R染色体发生突变,取代了普通小麦品种1B染色体。小麦-黑麦染色体1R的易位和缺失在10%以上的植株中被发现,并且大多数在后代中稳定地遗传。讨论了该系统在小麦育种中诱导小麦外源易位的可能用途。
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引用次数: 42
Disomic behavior of polyploid tuber-bearing Solanum species 多倍体龙葵种的二体行为
Pub Date : 1994-01-01 DOI: 10.1266/JJG.69.637
Kazuo N. Watanabe, M. Orrillo
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引用次数: 7
GENETIC STUDIES ON HYBRID STERILITY IN BARLEY 大麦杂交不育性的遗传研究
Pub Date : 1994-01-01 DOI: 10.1266/JJG.69.719
Y. Fukushima, T. Konishi
Partially sterile F1 barley plants were found when some Ethiopian varieties were crossed with Tayeh 1 (Chinese landrace), even though the parents were completely fertile. This hybrid sterility is shown to be controlled by two duplicate genes (sfg1 and sfg2). Because this gene interaction affects the female gamete only, the female gametes carrying the both sfg1 and sfg2 genes became sterile, the end result being that seed fertility of the F1 plants is decreased to around 75%. Seed fertility was not significantly influenced by cytoplasms and genetic backgrounds of the F1 plants, and years grown the F1 plants.
当一些埃塞俄比亚品种与Tayeh 1(中国地方品种)杂交时,发现部分不育的F1大麦植株,尽管亲本是完全可育的。这种杂交不育性是由两个重复基因(sfg1和sfg2)控制的。由于这种基因相互作用只影响雌性配子,因此同时携带sfg1和sfg2基因的雌性配子变得不育,最终结果是F1植株的种子育性下降到75%左右。籽粒育性不受细胞质、遗传背景和生长年限的显著影响。
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引用次数: 1
Induction of parthenogenetic haploid plants with brassinolide 油菜素内酯诱导孤雌单倍体植株的研究
Pub Date : 1994-01-01 DOI: 10.1266/JJG.69.35
Y. Kitani
A plant steroid hormone, brassinolide, was found to induce parthenogenetic haploid seed generation when applied to the stigmas of the castrated flower buds. In the two species examined, Arabidopsis thaliana and Brassica juncea, haploid seeds were obtained which developed to stable haploid plants, but in the third species, Tradescantia paludosa, haploid seeds when germinated soon became haplo-diplo mixoploid and then diploid.
一种植物类固醇激素,油菜素内酯,当施用于去势花蕾的柱头时,可诱导单性生殖单倍体种子产生。拟南芥(Arabidopsis thaliana)和芥菜(Brassica juncea)获得的单倍体种子发育为稳定的单倍体植株,而第三种植物Tradescantia paludosa的单倍体种子在萌发后很快变成单倍体-二倍体混合倍体,然后变成二倍体。
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引用次数: 12
Different expression in MDH isozymes among local populations in freshwater shrimp, Paratya compressa (Decapoda: Atyidae) 淡水对虾MDH同工酶在当地种群中的表达差异(十足目:无虾科)
Pub Date : 1994-01-01 DOI: 10.1266/JJG.69.679
M. Ikeda, A. Kijima, Y. Fujio
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引用次数: 3
Homoeologous group 1 chromosomes of Agropyron restore nucleus-cytoplasmic compatibility in alloplasmic common wheat with Agropyron cytoplasms 在异质性普通小麦中,Agropyron的同源1组染色体恢复了Agropyron细胞质的核-细胞质相容性
Pub Date : 1994-01-01 DOI: 10.1266/JJG.69.41
Tohru Suzuki, C. Nakamura, N. Mori, Y. Iwasa, C. Kaneda
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引用次数: 9
期刊
The Japanese Journal of Genetics
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