Mature eggs dissected from the ovary of unmated sawflies (Tenthredinidae, Hymenoptera) can be activated to develop (to haploid males), simply by placing on a filter paper wet with distilled water. These unfertilized eggs may be injected with sperm, and some, successfully completing fertilization, develop as diploid females. Premating reproductive isolation exists between two sympatric species of this family, Athalia rosae ruficornis Jokovlev and Athalia infumata Marlatt. Taking advantage of the difference in karyotypes, it is shown that hetero-specific sperm injection results in successful fertilization and that the hybrid survives at least through the middle stage of embryogenesis.
{"title":"Fertilization of hetero-specific insect eggs by sperm injection","authors":"M. Sawa","doi":"10.1266/JJG.66.297","DOIUrl":"https://doi.org/10.1266/JJG.66.297","url":null,"abstract":"Mature eggs dissected from the ovary of unmated sawflies (Tenthredinidae, Hymenoptera) can be activated to develop (to haploid males), simply by placing on a filter paper wet with distilled water. These unfertilized eggs may be injected with sperm, and some, successfully completing fertilization, develop as diploid females. Premating reproductive isolation exists between two sympatric species of this family, Athalia rosae ruficornis Jokovlev and Athalia infumata Marlatt. Taking advantage of the difference in karyotypes, it is shown that hetero-specific sperm injection results in successful fertilization and that the hybrid survives at least through the middle stage of embryogenesis.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"15 1","pages":"297-303"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90947601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Forty-six stable (21II) hexaploid plants were cytologically screened in the F5 generation of a cross between a hexaploid triticale cv. Armadillo (2D/2R substitution type) and a bread wheat cv. Chinese Spring. In order to determine the constitution of R- and D-genome chromosomes of the stable F5 plants, F6 progeny of each F5 plant was analyzed by C-banding. Of the 46 plants, 43 had no translocation, while one was homozygous and two were heterozygous for translocation. Of the theoretically possible 26 = 64 kinds of chromosome constitutions, only 12 kinds were obtained in the stable plants without translocation. They had zero to six pairs of R-genome chromosomes and appeared with different frequencies. Frequently observed chromosome constitutions, which independently originated from many F2 progenitors, had one, four, five or six pairs of R-genome chromosomes. Chromosomes 1R, 3R and 6R were independently replaced by their respective homoeologous D-genome chromosomes. Chromosomes 4R, 5R and 7R always behaved together except in two infrequent chromosome constitutions in which 5R was separated from 4R and 7R. From the information so far reported about the homoeologous relationship between rye and wheat chromosomes, we inferred that the incomplete homoeology of 4R, 5R and 7R to the corresponding homoeologous D-genome chromosomes was responsible for the concurrent presence or absence of these three R-genome chromosomes.
{"title":"Preferential occurrence of specific R-D chromosome constitutions in stable hexaploid progenies of the hybrid between hexaploid triticale and bread wheat","authors":"S. Taketa, T. Nakazaki, S. Shigenaga, H. Yamagata","doi":"10.1266/JJG.66.587","DOIUrl":"https://doi.org/10.1266/JJG.66.587","url":null,"abstract":"Forty-six stable (21II) hexaploid plants were cytologically screened in the F5 generation of a cross between a hexaploid triticale cv. Armadillo (2D/2R substitution type) and a bread wheat cv. Chinese Spring. In order to determine the constitution of R- and D-genome chromosomes of the stable F5 plants, F6 progeny of each F5 plant was analyzed by C-banding. Of the 46 plants, 43 had no translocation, while one was homozygous and two were heterozygous for translocation. Of the theoretically possible 26 = 64 kinds of chromosome constitutions, only 12 kinds were obtained in the stable plants without translocation. They had zero to six pairs of R-genome chromosomes and appeared with different frequencies. Frequently observed chromosome constitutions, which independently originated from many F2 progenitors, had one, four, five or six pairs of R-genome chromosomes. Chromosomes 1R, 3R and 6R were independently replaced by their respective homoeologous D-genome chromosomes. Chromosomes 4R, 5R and 7R always behaved together except in two infrequent chromosome constitutions in which 5R was separated from 4R and 7R. From the information so far reported about the homoeologous relationship between rye and wheat chromosomes, we inferred that the incomplete homoeology of 4R, 5R and 7R to the corresponding homoeologous D-genome chromosomes was responsible for the concurrent presence or absence of these three R-genome chromosomes.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"48 1","pages":"587-596"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89844200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Toshihiko Imai, S. Ichikawa, Marie Sanda-Kamigawara
Variation of spontaneous somatic pink mutation frequency was studied in the stamen hairs of Tradescantia KU 20 clone, a highly mutable blue/pink heterozygote. The spontaneous mutation frequency varied greatly from 4.03±1.21 to 120±7 and from 18.8±3.1 to 110±5 pink mutant events per 103 hairs when the plants were grown outdoors and in the greenhouse, respectively, being generally higher at lower temperature and also in the greenhouse than in outdoors. The spontaneous mutation frequency under controlled environmental conditions also varied from 3.06±0.37 to 40.8±3.1 pink mutant events per 103 hairs, showing a clearer negative correlation with temperature. It was found that the spontaneous mutation frequency under controlled environmental conditions increased when day/night temperature shifts were applied, especially with a 5°C shift than with 3°C shifts. The difference between the highest and the lowest mutation frequencies reached almost 40 times, and this clone was confirmed to be a temperature-sensitive mutable clone. A repair mechanism of DNA damages occurring spontaneously, which is more effective at higher temperature, thus presumably an enzymatic one, is very likely involved in the mutable nature of this clone.
以高变异蓝粉杂合子Tradescantia KU 20为材料，对其雄蕊自发体细胞粉红突变频率进行了研究。在室外和温室中，每103根毛羽的粉红突变次数分别为4.03±1.21 ~ 120±7次和18.8±3.1 ~ 110±5次，在低温和温室中均高于室外。可控环境条件下的自发突变频率为3.06±0.37 ~ 40.8±3.1次/ 103根，与温度呈明显负相关。研究发现，在受控环境条件下，当昼夜温差变化时，自发突变频率增加，特别是当温度变化5°C时，突变频率高于3°C时。最高和最低突变频率之间的差异达到近40倍，该克隆被证实是温度敏感的可变克隆。一种自发发生的DNA损伤修复机制，在更高的温度下更有效，因此可能是一种酶的修复机制，很可能与这种克隆的易变性有关。
{"title":"Variation of spontaneous somatic mutation frequency in the stamen hairs of a mutable clone of Tradescantia, KU 20","authors":"Toshihiko Imai, S. Ichikawa, Marie Sanda-Kamigawara","doi":"10.1266/JJG.66.501","DOIUrl":"https://doi.org/10.1266/JJG.66.501","url":null,"abstract":"Variation of spontaneous somatic pink mutation frequency was studied in the stamen hairs of Tradescantia KU 20 clone, a highly mutable blue/pink heterozygote. The spontaneous mutation frequency varied greatly from 4.03±1.21 to 120±7 and from 18.8±3.1 to 110±5 pink mutant events per 103 hairs when the plants were grown outdoors and in the greenhouse, respectively, being generally higher at lower temperature and also in the greenhouse than in outdoors. The spontaneous mutation frequency under controlled environmental conditions also varied from 3.06±0.37 to 40.8±3.1 pink mutant events per 103 hairs, showing a clearer negative correlation with temperature. It was found that the spontaneous mutation frequency under controlled environmental conditions increased when day/night temperature shifts were applied, especially with a 5°C shift than with 3°C shifts. The difference between the highest and the lowest mutation frequencies reached almost 40 times, and this clone was confirmed to be a temperature-sensitive mutable clone. A repair mechanism of DNA damages occurring spontaneously, which is more effective at higher temperature, thus presumably an enzymatic one, is very likely involved in the mutable nature of this clone.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"26 1","pages":"501-511"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74918597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mitochondrial (mt) and chloroplast (ct) DNA were characterized in somatic hybrids obtained by fusion of protoplasts from two species, Nicotiana glauca and N. langsdorffii. Three and a half months after the fusion, total DNA was extracted from 50-100 mg of callus and of leaf tissue. Southern hybridization was carried out with several probes from organelle DNA. The results revealed that widespread rearrangements of mtDNA had occurred in the majority of hybrid calli. Even subclones derived from the same original hybrid callus generated different patterns of rearrangements. In contrast to our results for calli, we found that leaves contained almost a simple parental-type mtDNA, namely, that of N. langsdorffii in this experiment, and few rearrangements of mtDNA were detected. Data obtained with cob as probe distinctly showed the difference between calli and leaves. These results suggest that there may be a correlation between the organization of mtDNA in hybrids and the ability of hybrids to regenerate, since nuclear genomes of hybrids are known to be stable. Segregation of two kinds of ctDNA was observed more frequently in leaves than in calli. It was also apparent that there was no correlation between species of chloroplasts and species of mitochondria since we found a heterogeneous component of organelles.
{"title":"The heterogeneous composition of mitochondrial DNA in somatic hybrid calli and the relatively simple composition of such DNA in regenerated leaves","authors":"H. Honda, K. Itoh, A. Hirai","doi":"10.1266/JJG.66.279","DOIUrl":"https://doi.org/10.1266/JJG.66.279","url":null,"abstract":"Mitochondrial (mt) and chloroplast (ct) DNA were characterized in somatic hybrids obtained by fusion of protoplasts from two species, Nicotiana glauca and N. langsdorffii. Three and a half months after the fusion, total DNA was extracted from 50-100 mg of callus and of leaf tissue. Southern hybridization was carried out with several probes from organelle DNA. The results revealed that widespread rearrangements of mtDNA had occurred in the majority of hybrid calli. Even subclones derived from the same original hybrid callus generated different patterns of rearrangements. In contrast to our results for calli, we found that leaves contained almost a simple parental-type mtDNA, namely, that of N. langsdorffii in this experiment, and few rearrangements of mtDNA were detected. Data obtained with cob as probe distinctly showed the difference between calli and leaves. These results suggest that there may be a correlation between the organization of mtDNA in hybrids and the ability of hybrids to regenerate, since nuclear genomes of hybrids are known to be stable. Segregation of two kinds of ctDNA was observed more frequently in leaves than in calli. It was also apparent that there was no correlation between species of chloroplasts and species of mitochondria since we found a heterogeneous component of organelles.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"6 1","pages":"279-289"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76056637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Nou, Masao Watanabe, A. Isogai, H. Shiozawa, A. Suzuki, K. Hinata
Variation of S-alleles and S-glycoproteins associated with self-incompatibility was studied in a naturalized population of Brassica campestris growing in Oguni-machi, Japan. Of 58 plants collected from the population, 45 were self-incompatible and 8 were self-compatible. Of 32 families investigated on their selfed progenies, 30 families showed segregation fitting to one locus S-allele model of sporophytic self-incompatibility. From cross-pollination experiments between 35 S-homozygotes so far isolated, 16 different S-alleles were identified, and the number of S-alleles involved in this population was estimated to be 20-30. S-glycoproteins corresponding to each S-allele were determined by immunoblotting with polyclonal antibody against S8-glycoprotein. Concanavalin A and Coomassie Blue stainings were also applied to determining corresponding S-glycoproteins, but were not so clear as the antibody cross reaction. It is pointed out that a stigma involves a number of proteins with different pI points, which are cross-reactive with anti-S-glycoprotein-antiserum. Many of these proteins are heritable in correlation with major S-glycoproteins. Since the content of these proteins was variable, we tentatively classified major and minor S-glycoproteins, and assumed that these S-glycoproteins were controlled by S-like DNA sequences with closely linked S-locus. Beside these S-glycoproteins, presence of heterozygote specific proteins was also pointed out, suggesting occurrence of post-transcriptional modification of these proteins. The pI values of major S-glycoproteins ranged from 5.0-9.0 and those at 7.0-9.0 were frequent.
{"title":"Variation of S-alleles and S-glycoproteins in a naturalized population of self-incompatible Brassica campestris L.","authors":"I. Nou, Masao Watanabe, A. Isogai, H. Shiozawa, A. Suzuki, K. Hinata","doi":"10.1266/JJG.66.227","DOIUrl":"https://doi.org/10.1266/JJG.66.227","url":null,"abstract":"Variation of S-alleles and S-glycoproteins associated with self-incompatibility was studied in a naturalized population of Brassica campestris growing in Oguni-machi, Japan. Of 58 plants collected from the population, 45 were self-incompatible and 8 were self-compatible. Of 32 families investigated on their selfed progenies, 30 families showed segregation fitting to one locus S-allele model of sporophytic self-incompatibility. From cross-pollination experiments between 35 S-homozygotes so far isolated, 16 different S-alleles were identified, and the number of S-alleles involved in this population was estimated to be 20-30. S-glycoproteins corresponding to each S-allele were determined by immunoblotting with polyclonal antibody against S8-glycoprotein. Concanavalin A and Coomassie Blue stainings were also applied to determining corresponding S-glycoproteins, but were not so clear as the antibody cross reaction. It is pointed out that a stigma involves a number of proteins with different pI points, which are cross-reactive with anti-S-glycoprotein-antiserum. Many of these proteins are heritable in correlation with major S-glycoproteins. Since the content of these proteins was variable, we tentatively classified major and minor S-glycoproteins, and assumed that these S-glycoproteins were controlled by S-like DNA sequences with closely linked S-locus. Beside these S-glycoproteins, presence of heterozygote specific proteins was also pointed out, suggesting occurrence of post-transcriptional modification of these proteins. The pI values of major S-glycoproteins ranged from 5.0-9.0 and those at 7.0-9.0 were frequent.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"33 1","pages":"227-239"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83836215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Partial mono-trisomic plants of rice, Oryza sativa L. derived from interchange homozygote, RT2-3b•T65","authors":"Shigetoshi Sato","doi":"10.1266/JJG.66.117","DOIUrl":"https://doi.org/10.1266/JJG.66.117","url":null,"abstract":"","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"22 1","pages":"117-128"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85680340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In order to detect DNA replication in chromosomes of four plant species, Allium fistulosum (2n=16), Crepis capillaris (2n=6), Haplopappus gracilis (2n=4) and Chrysanthemum coronarium var. spatiosum (2n=18), an immunological technique using monoclonal anti-BrdU antibody was utilized. In this technique, HCl was used for denaturation and avidin-biotin complex for the immunological process. The replicating bands which appeared with this technique were of unusually high resolution.
{"title":"Visualization of replicating bands in plant chromosomes with a monoclonal anti-BrdU antibody method","authors":"K. Taniguchi, R. Tanaka","doi":"10.1266/JJG.66.485","DOIUrl":"https://doi.org/10.1266/JJG.66.485","url":null,"abstract":"In order to detect DNA replication in chromosomes of four plant species, Allium fistulosum (2n=16), Crepis capillaris (2n=6), Haplopappus gracilis (2n=4) and Chrysanthemum coronarium var. spatiosum (2n=18), an immunological technique using monoclonal anti-BrdU antibody was utilized. In this technique, HCl was used for denaturation and avidin-biotin complex for the immunological process. The replicating bands which appeared with this technique were of unusually high resolution.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"27 1","pages":"485-489"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84809548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To compare the gene order of chloroplast genome among monocotyledonous plants, we constructed a physical map of chloroplast DNA (cpDNA) of onion (Allium cepa L. cv. Shounan red, 2n=16) with four restriction endonucleases, PstI, SalI, XhoI, and SmaI. The cpDNA of Allium cepa was found to be a circular molecule with a total size of ca. 155 kb and containing two inverted repeats of 26 kb each that disrupt the rest of the molecule into small (ca. 16 kb) and large (ca. 86 kb) single copy regions. The endonuclease recognition sites of the physical map were confirmed by Southern hybridizations of total onion cpDNA with homologous and heterologous probes. 16 genes were localized on the physical map of the onion cpDNA. Genome structure of onion cpDNA was similar in terms of genome size and gene order of cpDNA to that of tobacco cpDNA, differing from the cpDNA structure of gramineous plants.
{"title":"Physical map of chloroplast DNA of the onion Allium cepa L., showing the location of photosynthesis-related genes","authors":"H. Katayama, T. Sasakuma, Y. Ogihara","doi":"10.1266/JJG.66.421","DOIUrl":"https://doi.org/10.1266/JJG.66.421","url":null,"abstract":"To compare the gene order of chloroplast genome among monocotyledonous plants, we constructed a physical map of chloroplast DNA (cpDNA) of onion (Allium cepa L. cv. Shounan red, 2n=16) with four restriction endonucleases, PstI, SalI, XhoI, and SmaI. The cpDNA of Allium cepa was found to be a circular molecule with a total size of ca. 155 kb and containing two inverted repeats of 26 kb each that disrupt the rest of the molecule into small (ca. 16 kb) and large (ca. 86 kb) single copy regions. The endonuclease recognition sites of the physical map were confirmed by Southern hybridizations of total onion cpDNA with homologous and heterologous probes. 16 genes were localized on the physical map of the onion cpDNA. Genome structure of onion cpDNA was similar in terms of genome size and gene order of cpDNA to that of tobacco cpDNA, differing from the cpDNA structure of gramineous plants.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"37 4 1","pages":"421-431"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84296466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A system for long-term dry preservation of calli of rice (Oryza sativa L.) and a protection mechanism regulating the survival of dried calli were investigated. The highest survival of dried calli and the highest regeneration rate of plantlets were observed in calli which had been pretreated with 10-5 M abscisic acid (ABA) in the presence of 90 g/l of sucrose and were regrown on an R-2 medium. We detected a corresponding accumulation of the transcript RNA of the rab 16A gene (a rice gene induced by ABA and water stress) in dried calli, mature seeds, and calli pretreated with 10-5 M ABA. The levels of this mRNA increased with the increase of the sucrose concentation, indicating that the accumulation of rab 16A mRNA is regulated by ABA at higher concentrations of sucrose and related to the survival of dried calli. Analysis of proteins by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) demonstrated similar protein patterns in fresh and dried calli. However, in the dried calli pretreated with 10-5 M ABA and 90 g/l of sucrose, different protein patterns were found compared to those in a callus dried without the pretreatment, indicating that some specific polypeptides might be synthesized in the pretreated dried callus.
研究了水稻愈伤组织的长期干燥保存体系和调节干燥愈伤组织存活的保护机制。在蔗糖浓度为90 g/l的条件下，用10-5 M的脱落酸(ABA)预处理愈伤组织，在R-2培养基上再生，愈伤组织的干燥成活率最高，植株再生率最高。我们在干燥愈伤组织、成熟种子和10-5 M ABA预处理的愈伤组织中检测到rabb 16A基因(ABA和水分胁迫诱导的水稻基因)转录RNA的相应积累。该mRNA的表达量随着蔗糖浓度的增加而增加，说明在高浓度蔗糖条件下，ABA对兔16A mRNA的积累进行调控，并与干愈伤组织的存活有关。通过二维聚丙烯酰胺凝胶电泳(2D-PAGE)分析，发现新鲜和干燥愈伤组织的蛋白质模式相似。但经10-5 M ABA和90 g/l蔗糖处理后的愈伤组织与未处理的愈伤组织相比，其蛋白质结构有所不同，说明预处理后的愈伤组织可能合成了一些特定的多肽。
{"title":"Survival mechanism of dried calli and regeneration of plants in rice","authors":"D. Shin, S. Virigool, K. Shinozaki, K. Oono","doi":"10.1266/JJG.66.13","DOIUrl":"https://doi.org/10.1266/JJG.66.13","url":null,"abstract":"A system for long-term dry preservation of calli of rice (Oryza sativa L.) and a protection mechanism regulating the survival of dried calli were investigated. The highest survival of dried calli and the highest regeneration rate of plantlets were observed in calli which had been pretreated with 10-5 M abscisic acid (ABA) in the presence of 90 g/l of sucrose and were regrown on an R-2 medium. We detected a corresponding accumulation of the transcript RNA of the rab 16A gene (a rice gene induced by ABA and water stress) in dried calli, mature seeds, and calli pretreated with 10-5 M ABA. The levels of this mRNA increased with the increase of the sucrose concentation, indicating that the accumulation of rab 16A mRNA is regulated by ABA at higher concentrations of sucrose and related to the survival of dried calli. Analysis of proteins by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) demonstrated similar protein patterns in fresh and dried calli. However, in the dried calli pretreated with 10-5 M ABA and 90 g/l of sucrose, different protein patterns were found compared to those in a callus dried without the pretreatment, indicating that some specific polypeptides might be synthesized in the pretreated dried callus.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"98 1","pages":"13-25"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77296014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hiroaki Suzuki, Y. Futsuhara, F. Takaiwa, N. Kurata
The location of storage protein glutelin gene on a chromosome was determined by in situ hybridization made between glutelin cDNA clone and mitotic chromosomes in rice. After hybridization, the location of 3H-labelled glutelin gene was analyzed on chromosomes of twenty one cells. It was found that silver grains were mainly concentrated on the short arm of chromosome 2 (K2 chromosome), demonstrating that the gene responsible for storage protein glutelin was located on this chromosome.
{"title":"Localization of glutelin gene in rice chromosome by in situ hybridization","authors":"Hiroaki Suzuki, Y. Futsuhara, F. Takaiwa, N. Kurata","doi":"10.1266/JJG.66.305","DOIUrl":"https://doi.org/10.1266/JJG.66.305","url":null,"abstract":"The location of storage protein glutelin gene on a chromosome was determined by in situ hybridization made between glutelin cDNA clone and mitotic chromosomes in rice. After hybridization, the location of 3H-labelled glutelin gene was analyzed on chromosomes of twenty one cells. It was found that silver grains were mainly concentrated on the short arm of chromosome 2 (K2 chromosome), demonstrating that the gene responsible for storage protein glutelin was located on this chromosome.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"1 1","pages":"305-312"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83178430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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