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PARASITE FAUNA OF LABORATORY MICE 实验小鼠的寄生虫动物群
Pub Date : 2023-04-17 DOI: 10.31016/978-5-6048555-6-0.2023.24.376-380
D. Polukhina, O. Panova, O. Kurnosova
Mice are widely used as laboratory animals. Diagnosis of endoparasites is a necessary condition: this is a culling indicator of animals for SPF (specific pathogen free) vivariums, and for conventional vivariums, it is the necessary information for planning and the possibility of using these animals in various tests. This article describes live-time diagnostics of endoparasites in laboratory mice in SPF vivariums and in open-type vivariums. For the study, individual samples of faeces and bedding were taken from laboratory mice. Direct fecal smears were examined and the flotation method was used. The work was carried out in 2021–2022 in the Laboratory of Biology and Biological Basis of Preventive Measures of the VNIIP – FSC VIEV. During this study, the following 180 samples were taken: 138 individual faecal samples, 34 samples combined with the bedding, and 18 bedding samples. The studied samples were found to contain Giardia muris and Tritrichomonas sp., nematodes Aspiculuris tetraptera and Syphacia obvelata, and cestode Rodentolepis nana. Mixed invasions were also recorded. The results showed the need for planning work with laboratory mice in vivariums.
老鼠被广泛用作实验动物。内寄生虫的诊断是一个必要条件:这是SPF(无特定病原体)家养动物的扑杀指标,对于常规家养动物,这是规划和使用这些动物进行各种试验的可能性的必要信息。本文介绍了SPF型和开放式实验小鼠体内寄生虫的实时诊断方法。在这项研究中,从实验室老鼠身上提取了粪便和床上用品的样本。直接粪便涂片检查,采用浮选法。这项工作于2021-2022年在VNIIP - FSC VIEV生物学和预防措施生物学基础实验室进行。本研究共采集了以下180个样本:138个个体粪便样本,34个与床上用品结合的样本,18个床上用品样本。检出鼠贾第鞭毛虫、毛滴虫,四目弓形线虫、扁螺旋体线虫和小啮齿动物。混合入侵也有记录。结果表明,需要在实验室小鼠体内进行计划工作。
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引用次数: 0
TOXOPLASMOSIS AMONG THE POPULATION OF THE KRASNODAR TERRITORY 克拉斯诺达尔地区人口中的弓形虫病
Pub Date : 2023-04-17 DOI: 10.31016/978-5-6048555-6-0.2023.24.544-548
L. N. Shipkova, N. V. Shipkov, S. V. Kalmanovich, A. K. Cherednichenko
The existing interest in the problem of toxoplasmosis is largely due to the fact that Toxoplasma gondii plays a significant role in human pathology. Toxoplasmas enter the human body in many ways: through the mouth when eating raw meat; with contaminated products, vegetables or fruits; with dirty hands, after contact with infected animals; through damaged skin, mucous membranes, when removing skins from infected animals or dividing carcasses; in medical procedures in patients with toxoplasmosis; and prenatally, from mother to fetus. Toxoplasmosis is more common in children, while adults may remain asymptomatic carriers of the pathogen. The reliability of invasion in humans is only established by the detection of toxoplasma in the blood, sputum, cerebrospinal fluid, puncture samples of lymph nodes, remnants of fetal membranes, placenta, etc. Therefore, the purpose of our study is to determine the occurrence of toxoplasmosis in the Krasnodar Territory for 5 years (2018–2022) and to identify organ systems that are affected most often. Toxoplasmosis is widespread in the Krasnodar Territory. This is due to climatic and geographical features. The study used data from outpatient medical records of people with diagnosed toxoplasmosis who seek medical advice in infectious diseases hospitals of the Krasnodar Territory.
人们对弓形虫病问题的兴趣很大程度上是由于弓形虫在人类病理中起着重要作用。弓形虫通过多种途径进入人体:吃生肉时通过口腔;受污染的产品、蔬菜或水果;接触受感染动物后双手不洁;在切除受感染动物的皮肤或分割尸体时,通过受损的皮肤、粘膜;在弓形虫病患者的医疗程序中;在产前,从母亲传给胎儿。弓形虫病在儿童中更为常见,而成人可能仍然是病原体的无症状携带者。只有在血液、痰液、脑脊液、淋巴结穿刺样本、胎膜残余、胎盘等中检测到弓形虫,才能确定人类入侵的可靠性。因此,我们研究的目的是确定克拉斯诺达尔地区5年(2018-2022年)弓形虫病的发生情况,并确定最常受影响的器官系统。弓形虫病在克拉斯诺达尔地区很普遍。这是由于气候和地理的特点。该研究使用了在克拉斯诺达尔地区传染病医院就诊的确诊弓形虫病患者的门诊病历数据。
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引用次数: 0
DETECTION OF POSTHODIPLOSTOMUM CUTICOLA (NORDMANN, 1832) IN FISH IN THE WATER BODIES OF THE NOVOSIBIRSK REGION 新西伯利亚地区水体中鱼类表皮裂口病(nordmann, 1832)的检测
Pub Date : 2023-04-17 DOI: 10.31016/978-5-6048555-6-0.2023.24.100-104
O. M. Bonina, E. A. Udaltsov, M. S. Bortsova
The article presents data on infection of fish of the cyprinid family (ide, dace, roach) with metacercariae of trematode Posthodiplostomum cuticola. Fish for the study were caught in the following water bodies of the Novosibirsk Region: the Chulym, Karakan, Inya, and Makhalikha Rivers, as well as from the left (Sharapovsky Bay) and right (Tulkinsky Bay) banks of the Novosibirsk Reservoir. Fish were studied by the compressor method generally accepted in parasitology and by partial helminthological dissection. A total of 270 fish specimens (71 ides, 82 daces, and 117 roaches) were studied. To analyze the fish infection, such indicators as the invasion prevalence and intensity, as well as the abundance index were used. The research results showed that the overall level of infection of cyprinids with P. cuticola metacercariae in the reservoirs of the Novosibirsk Region was 13.0%. The highest invasion prevalence of 35.2% was observed in ides; in daces and roaches this value is much lower and amounts to 7.3% and 3.4%, respectively. The invasion intensity was low, ranging from 1 to 7 parasite specimens per fish. The highest average invasion intensity of 3.3 and 3.1 specimens was recorded in daces from the Karakan River and ides from the Chulym River.
本文报道了鲤科鱼类(鲤科、鲤科、蟑螂科)感染表皮吸虫后囊蚴的资料。研究中的鱼是在新西伯利亚地区的以下水体中捕获的:丘利姆河、卡拉干河、因亚河和马哈利哈河,以及新西伯利亚水库的左岸(Sharapovsky湾)和右岸(Tulkinsky湾)。采用寄生虫学中普遍采用的压缩法和部分蠕虫解剖法对鱼类进行了研究。共采集鱼类标本270份,其中鱼类71种,鱼类82种,蟑螂117种。采用入侵率、入侵强度、丰度等指标分析鱼类侵染情况。研究结果表明,新西伯利亚地区水库中鲤类感染表皮棘球蚴的总体水平为13.0%。ides的侵袭率最高,为35.2%;在蟾蜍和蟑螂中,这一数值要低得多,分别为7.3%和3.4%。侵染强度较低,每条鱼1 ~ 7条。平均入侵强度最高的是喀喇干河和楚林河,分别为3.3和3.1个标本。
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引用次数: 0
CASES OF REGISTRATION OF LARVAL CESTODIASIS OF SHEEP AT A SLAUGHTERHOUSE IN THE MOSCOW REGION 莫斯科地区屠宰场绵羊幼虫囊虫病登记病例
Pub Date : 2023-04-17 DOI: 10.31016/978-5-6048555-6-0.2023.24.503-507
I. I. Tsepilova, B. K. Laipanov, S. A. Shemyakova
On the territory of the slaughterhouse located in the Moscow Region, a veterinary and sanitary assessment of carcasses and internal organs was carried out in accordance with the Customs Union Technical Regulations "On the safety of meat and meat products" No. 68 TR CU 034/2013 dated 09/10/2013 for the infection with larval cestodiasis. As a result, 3 species of pathogens were recorded: Echinococcus granulosus, Cysticercus taenuicollis and Coenurus cerebralis. E. granulosus larvae was detected in the parenchymal organs in 211 cases, which corresponds to 42.2%. It is worth noting that the blister cavities were localized in the liver in 23.7%, in the lungs in 15.6%, and in both organs in 2.9%. From 1 to 8 blisters were found. In addition to echinococcosis, coenurus blister cavities were found in 2 animals, and Cysticercus tenuicollis, in 22 sheep from the Karachay-Cherkess Republic; the IP was 2.0 and 22.4%, and tenuicollic cysticercosis infection in the Astrakhan and Volgograd Regions was 2.9 and 6.0%, respectively. Such a wide spread of echinococcosis is a fairly high indicator for such a particularly dangerous zoonosis, as parenchymal organs can be a source of infection for dogs, and subsequently for humans.
在位于莫斯科州的屠宰场境内,根据2013年10月9日第68 TR CU 034/2013号关税同盟技术法规“关于肉类和肉类产品的安全”,对尸体和内脏进行了兽医和卫生评估,以确定是否感染幼虫囊虫病。结果共检出细粒棘球蚴、带状囊尾蚴和脑棘球蚴3种病原体。211例肺实质器官检出细粒绦虫幼虫,占42.2%。值得注意的是,23.7%的水疱空洞位于肝脏,15.6%位于肺部,2.9%位于两个器官。发现1 ~ 8个水泡。除棘球蚴病外,在2只动物中发现了棘球蚴水疱,在卡拉恰伊-切尔克斯共和国的22只羊中发现了细囊尾蚴;感染率分别为2.0和22.4%,阿斯特拉罕和伏尔加格勒地区的细粒囊尾蚴病感染率分别为2.9和6.0%。棘球蚴病的如此广泛传播是这种特别危险的人畜共患病的一个相当高的指标,因为实质器官可能是狗的感染源,随后是人类的感染源。
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引用次数: 0
HELMINTHS, PROTOZOAN PARASITES AND HELMINTHOZOONOSIS IN DOMESTIC DOGS OF DIFFERENT AGE GROUPS IN MOSCOW 莫斯科不同年龄组家养狗的寄生虫、原生动物寄生虫和寄生虫病
Pub Date : 2023-04-17 DOI: 10.31016/978-5-6048555-6-0.2023.24.354-358
V. Pasechnik
The main purpose of the work was to identify helminth and protozoan parasite infections and helminthozoonosis in domestic dogs of different age groups in Moscow. Coproovoscopic examinations were carried out during 2022 for dogs older than a year and puppies up to 6 months from 5 administrative districts of the City. Fecal samples were collected in defecation of dogs individually in a sterile container with the animal information on the label during dog walking by their owners. Infection of the dogs was found with the following zoonotic helminths: Toxocara canis, Toxascaris leonina, Dipylidium caninum, and Taenia spp.; and protozoa: Isospora canis. Toxocara canis reached its maximum value in the puppies up to 6 months of age with the prevalence of 33.3%, and in the dogs older than a year with the prevalence of 15.3%. Toxascaris leonina was not found in the puppies under 6 months of age; in the dogs older than a year, it reached the maximum invasion value with the prevalence of 9.5%. Dipylidium caninum reached the maximum invasion value in the puppies aged up to 6 months with the prevalence of 7.8%, but the dogs older than a year were not found to have Dipylidium caninum. Taenia spp., were found neither in the puppies nor in dogs older than a year.
本研究的主要目的是鉴定莫斯科不同年龄组家养狗的寄生虫和原生动物寄生虫感染及寄生虫病。在2022年期间,对来自该市5个行政区的一岁以上的狗和6个月以下的幼犬进行了coprovoscopy检查。在狗的主人遛狗时,将狗单独放在无菌容器中排便,并在标签上注明动物信息,收集粪便样本。犬感染人畜共患寄生虫:犬弓形虫、狮弓形虫、犬双螺旋虫和带绦虫;原生动物:犬等孢子虫。犬弓形虫在6个月以下的幼犬中患病率最高,为33.3%,在1岁以上的犬中患病率为15.3%。6月龄以下幼犬未检出狮子弓形虫;在1岁以上的犬中,达到最大侵袭值,患病率为9.5%。犬双螺旋绦虫在6个月以下的幼犬中达到最大侵袭值,患病率为7.8%,但一岁以上的犬未发现犬双螺旋绦虫。在幼犬和一岁以上的狗身上都没有发现带绦虫。
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引用次数: 0
NEMATICIDAL AND NEMATOSTATIC EFFICACY OF VEGETABLE JUICES OF SOME REPRESENTATIVES OF THE FAMILY SOLANACEAE 茄科几种代表性蔬菜汁的杀线虫和抑线虫效果
Pub Date : 2023-04-17 DOI: 10.31016/978-5-6048555-6-0.2023.24.220-224
A. Konrat
The effect of the tested substances on in vitro nematicidity and nematostaticity was verified on saprobiotic (Caenorhabditis elegans, Turbatrix aceti) and phytoparasitic nematodes (Ditylenchus destructor). Working solutions were prepared in the following dilutions: 1) 1/1000, 2) 1/100, 3) 1/10, 4) 1/1, 5) undiluted, 6) control, water, and 7) standard (horseradish root juice). The solution of above concentrations was introduced into the plate wells of 0.5 mL in 4 repetitions, then a well-mixed nematode suspension of 0.5 mL was added with 50±10 nematodes in average. After 24 hours of hold-times, mobile and immobile nematodes were counted, then after 48 hours nematodes were counted again. At the end of the experiment, nematodes were washed from the drug in order to determine the nematostatic effect. In vitro screening showed that the horseradish root juice, black nightshade berry juice, and red nightshade leaf and berry juice had nematicidal properties. The nematostatic effect was recorded in shoots of the green tuber, tissues of the green surface of the tuber and tuber juices, juice of red nightshade leaves, and juice of black nightshade leaves and berries.
并对腐生菌(秀丽隐杆线虫、乙酰Turbatrix)和植物寄生线虫(毁灭线虫)进行了体外杀线虫和抑线虫效果的验证。工作溶液按以下稀释度配制:1)1/1000,2)1/100,3)1/10,4)1/1,5)未稀释,6)对照,水,7)标准品(辣根汁)。将上述浓度的溶液加入0.5 mL的平板孔中,重复4次,然后加入均匀混合的0.5 mL线虫悬液,平均50±10只线虫。静置24 h后分别计数活动和不活动线虫,48 h后再次计数。在实验结束时,将线虫从药物中冲洗出来,以确定其抑线虫效果。体外筛选表明,辣根汁、黑茄莓汁和红茄叶莓汁具有杀线虫作用。绿色块茎的芽、块茎绿色表面组织和块茎汁液、红色龙葵叶汁液、黑色龙葵叶汁液和浆果汁液均有防线虫作用。
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引用次数: 0
COMPARATIVE EFFECTIVENESS OF GASTROS AND AVT-L TYPE DEVICES FOR TRICHINELLOSIS DIAGNOSTICS 胃和avt - 1型旋毛虫病诊断装置的比较效果
Pub Date : 2023-04-17 DOI: 10.31016/978-5-6048555-6-0.2023.24.487-492
A. Uspensky, L. Napisanova, O. Zhdanova, O. Andreyanov, N. S. Malysheva
The purpose of the research is to compare diagnostic and design characteristics of Gastros and AVT-L devices in order to determine the prospects for improving the veterinary and sanitary examination of carcasses with trichinellosis. Taking into account the requirements for ensuring the safety of food products, in particular meat and meat products, special requirements are imposed on the quality of trichinelloscopic control in the conditions of veterinary diagnostic laboratories of meat processing enterprises, markets and slaughterhouses. For this purpose, a fairly wide range of devices is used for both compressor trichinelloscopy and peptolysis of muscle tissue. Comparative tests were carried out for Gastros of Petrolaser LLC and AVT-L (VNIIP) type devices used for this purpose. In the process of work, we analyzed the features of the examination technology, the structural elements of the devices and the diagnostic efficiency. In general, the devices met the requirements of the veterinary and sanitary examination for trichinellosis in various production conditions, however, their design for manufacturability had significant differences. With equal diagnostic efficiency, the devices have significant design differences regarding the type of thermal stabilization, automation, control systems for the duration of the peptolysis and settling of the medium, and the dimensions of the devices. According to these parameters, Gastros is more promising at the present time. AVT-L requires some improvements.
本研究的目的是比较Gastros和AVT-L装置的诊断和设计特点,以确定改进旋毛虫病尸体兽医和卫生检查的前景。考虑到确保食品,特别是肉类和肉制品安全的要求,对肉类加工企业、市场和屠宰场的兽医诊断实验室的条件进行旋轮镜控制质量提出了特殊要求。为此目的,相当广泛的设备被用于压缩旋旋镜检查和肌肉组织的胃解。对Gastros of Petrolaser LLC和AVT-L (VNIIP)型设备进行了对比试验。在工作过程中,我们分析了检测技术的特点、设备的结构要素和诊断效率。总的来说,这些设备在各种生产条件下都满足旋毛虫病兽医和卫生检查的要求,但其可制造性设计存在显著差异。在诊断效率相同的情况下,这些设备在热稳定类型、自动化、培养基水解和沉淀期间的控制系统以及设备的尺寸方面存在显著的设计差异。根据这些参数,目前胃是比较有前途的。AVT-L需要一些改进。
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引用次数: 0
ACANTHOCEPHALANS IN WATERFOWLS OF LAKE BAIKAL 贝加尔湖水禽的棘头类
Pub Date : 2023-04-17 DOI: 10.31016/978-5-6048555-6-0.2023.24.67-72
D. R. Baldanova, T. R. Khamnueva, Z. Dugarov
The purpose of this work was to clarify the species composition of acanthocephalans of Baikal waterbirds and to determine their relative abundance in modern environmental conditions. Ninety nine specimens of 13 species of waterfowls were studied by the method of special and complete dissection in Lake Baikal. Birds were studied according to the method given in the work by M. N. Dubinina. Three species of acanthocephalans were recorded: Polymorphus minutus (Goeze, 1782), P. acutis Van Cleave et Starret, 1940, and P. magnus Skrjabin, 1913. To assess the quantitative parameters of host infection, the following indices were used: prevalence of invasion (iP), intensity of invasion (iI, limits) and abundance (A). P. minutus was the most widespread species of acanthocephalans. Acanthocephalans are localized in the small intestine of birds. Polymorphae penetrate very deeply into the small intestine wall of the host. Unlike echinorhynchids whose proboscis penetrates only into the submucosa of the intestinal mucosa of fish, the proboscis and neck of polymorphids pierce the mucous and muscular membranes reaching the serous membrane. Tubercles are observed on the outer, serous side of the intestine that mark places of attached acanthocephalans. With a high number of definitive hosts, they can potentially exert a regulatory influence on the number of bird populations.
本研究的目的是阐明贝加尔湖水鸟棘头目的种类组成,并确定其在现代环境条件下的相对丰度。对贝加尔湖的13种水禽进行了全面解剖,共99种标本。根据M. N. Dubinina工作中给出的方法对鸟类进行了研究。棘头类有3种:Polymorphus minutus (Goeze, 1782), P. acutis Van Cleave et Starret, 1940, P. magnus Skrjabin, 1913。采用入侵流行度(患病率)、入侵强度(极限)和丰度(A)等指标评价宿主感染的定量参数。棘头类动物局限于鸟类的小肠。多态菌深入宿主的小肠壁。与棘吻鱼不同,棘吻鱼的喙只穿过鱼肠粘膜的粘膜下层,多形鱼的喙和颈穿过粘膜和肌肉膜到达浆膜。在肠的外侧浆液面可见结节,标志着附着的棘头体。由于具有大量的最终宿主,它们可能对鸟类种群的数量施加调节影响。
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引用次数: 0
VETERINARY AND MEDICAL SIGNIFICANCE OF HORSEFLY (TABANIDAE). REVIEW 马蝇(虻科)的兽医和医学意义。审查
Pub Date : 2023-04-17 DOI: 10.31016/978-5-6048555-6-0.2023.24.133-137
F. Vasilevich, S. A. Shemyakova, N. Esaulova
The article provides information on the harmful effect of horseflies (Diptera, Tabanidae) as a midge component and a vector (carrier) transmitting pathogens of infectious and parasitic diseases including zoonosis. Horseflies are harmful to animals and humans in places of their abundance. Horseflies cause significant economic losses to livestock. With an intense attack of horseflies, individual areas of the skin of animals represent a continuous bleeding surface. Horsefly saliva inserted into a wound at the time of the bite is very toxic and allergenic causing a local inflammatory process and general intoxication of the body. The insects are of particular danger as vectors transmitting pathogens of animals and humans. The role of horseflies in the transmission of tularemia in natural foci of this infection has been proven. The sources of horsefly infection are primarily various small mammals including water rats. Horseflies are equally important as carriers of the anthrax pathogen. Causative agents of Coxiella burnetti infection, blackleg, pasteurellosis, and other infections have been isolated from horseflies. Horseflies are involved in the transmission of Trypanosoma evansi in horses and camels, anaplasmosis in cattle, Theileria cervi in reindeer, and equine infectious anemia virus.
本文介绍了马蝇(双翅目,虻科)作为蚊类和传播人畜共患病等传染病和寄生虫病病原体的媒介(载体)的危害作用。马蝇在其数量众多的地方对动物和人类都是有害的。马蝇对牲畜造成重大经济损失。在马蝇的猛烈攻击下,动物皮肤的个别区域呈现出持续流血的表面。马蝇唾液在被咬的时候插入伤口是非常有毒和过敏的,引起局部炎症过程和全身中毒。这些昆虫作为传播动物和人类病原体的媒介尤其危险。马蝇在土拉菌病自然疫源地传播中的作用已得到证实。马蝇感染的主要来源是各种小型哺乳动物,包括水鼠。马蝇作为炭疽病原体的携带者同样重要。从马蝇中分离出伯纳蒂克希氏菌感染、黑腿病、巴氏杆菌病和其他感染的病原体。马蝇参与传播马和骆驼中的伊文氏锥虫病、牛中的无形体病、驯鹿中的宫颈氏伊文氏杆菌和马传染性贫血病毒。
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引用次数: 0
BIODIVERSITY OF HELMINTHS OF THE DAGHESTANI TUR, CHAMOIS, AND ROE DEER IN THE SOUTH-EAST OF THE NORTH CAUCASUS 北高加索东南部达吉斯坦羚羊、岩羚羊和狍蠕虫的生物多样性
Pub Date : 2023-04-17 DOI: 10.31016/978-5-6048555-6-0.2023.24.58-61
A. Ataev, M. Zubairova, N. T. Karsakov
The ecosystems of the south-east of the Northern Caspian Sea are inhabited by three representatives of artiodactyls, namely, the Daghestani (East Caucasian) tur, the chamois (wild mountain goat) and the roe deer. All three species are under the state protection and listed in the Red Book. The helminth biodiversity in these three species of ruminants has been studied for 35 years due to the fact that the hunting is prohibited, therefore it is very difficult to collect material for research. The collected material analysis showed that helminth biodiversity in the Daghestani tur was represented by 20 species, chamois by 16 species, and roe deer by 15 species. From the trematode and cestode classes, 2 species of F. hepatica, D. lanceatum, M. expansa, and M. benedeni were recorded. The rest of the fauna was represented by nematodes including 22 species from the suborder Strongylata, one species of G. pulchrum from Spirurata and one species of T. ovis from Trichocephalata. The invasion prevalence (IP) of helminths in the Daghestani tur varied from 3.5 to 14.2% with the invasion intensity (II) of 1–12 specimens, respectively, in the chamois up to 8.3% and 2–5 specimens, and in the roe deer 4.1–8.3% and 1–4 specimens. The largest number of species were represented by the genera Nematodirus, Ostertagia, and Trichostrongylus.
北里海东南部的生态系统居住着三种偶蹄动物的代表,即Daghestani(东高加索)羚羊,cham羚羊(野生山地山羊)和狍子。这三个物种都是国家保护物种,并被列入红皮书。由于禁止狩猎,这三种反刍动物的蠕虫生物多样性研究已经进行了35年,因此很难收集到研究材料。收集到的资料分析表明,达吉斯坦地区蠕虫生物多样性有20种,岩羚羊有16种,狍子有15种。在吸虫类和绦虫类中,共记录到肝吸虫、长毛吸虫、扩张吸虫和贝氏吸虫2种。其余区系以线虫为代表,包括圆虫亚目22种、螺旋体目1种、毛头目1种。蚯蚓入侵率(IP)为3.5 ~ 14.2%,入侵强度(II)分别为1 ~ 12个样本,羚羊为8.3%和2 ~ 5个样本,狍为4.1 ~ 8.3%和1 ~ 4个样本。种类最多的是线虫属、尾螺属和毛线虫属。
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引用次数: 0
期刊
THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL
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