Pub Date : 2020-03-01DOI: 10.22271/tpi.2020.v9.i3g.4493
H. Bayan, K. Sarma, G. Rao, D. Kalita, D. Dutta, A. Phukan
{"title":"Propofol and ketamine CRI in dexmedetomidine and butorphanol premedicated dogs","authors":"H. Bayan, K. Sarma, G. Rao, D. Kalita, D. Dutta, A. Phukan","doi":"10.22271/tpi.2020.v9.i3g.4493","DOIUrl":"https://doi.org/10.22271/tpi.2020.v9.i3g.4493","url":null,"abstract":"","PeriodicalId":23030,"journal":{"name":"The Pharma Innovation Journal","volume":"31 1","pages":"334-337"},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84387758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-01DOI: 10.22271/tpi.2020.v9.i3b.4448
T. Manuroop, K Bharavi, A. Jahan, M Alpharaj, P. A. Kumar, G. S. Rao
Enrofloxacin in combination with phytochemicals such as Curcumin (CUR), Piperin (PIP), Cinnamic acid (CIA), Caffeic acid (CAA) and Syringic acid (SYA) exhibits notable synergism against pathogenic bacteria. Chitosan-alginate encapsulated microspheres containing enrofloxacin and phytochemicals prepared and evaluated for their synergistic effect and reduction in individual agent’s disadvantages. CSALG microspheres were prepared by impregnating enrofloxacin (CS-ALG-EN) alone and in combination with respective phytochemicals such as CS-ALG-EN-CUR, CS-ALG-EN-PIP, CS-ALG-EN-CIA, CSALG-EN-CAA & CS-ALG-EN-SYA and evaluated for shape, size, loading efficacy, release kinetics of enrofloxacin and MIC of enrofloxacin along with various phytochemicals against MTCC and clinical isolate bacteria. Microspheres were spherical. When combined with phytochemicals the enrofloxacin loading efficacy decreased variably with respective phytochemicals. The % cumulative release of enrofloxacin from all microspheres was maximum at pH 1.2 and further increased at pH 6.8. CAA and SYA improved the release and CIA, CUR and PIP decreased the release of enrofloxacin from respective microspheres compared to CS-ALG-EN. The dissolution efficacy increased by addition of SYA, CAA while PIP, CIA and CUR decreased. The mean dissolution time is same in PIP, SYA, CAA while CIA showed lowest and CUR highest when compared with enrofloxacin alone loaded microspheres. The release of enrofloxacin followed korsmeyer-peppas model by following Fickian diffusion/Quasi-Fickian diffusion from spheres. The MIC of enrofloxacin significantly lowered in combination with CUR, PIP, CIA, CAA on both MTCC and clinical isolates of pathogenic bacteria. In conclusion chitosan-alginate encapsulation improved the bioavailability of enrofloxacin and phytochemicals and combination showed synergistic antibacterial effect.
{"title":"Synthesis and evaluation of chitosan-alginate microspheres loaded with various combinations of enrofloxacin and selected phytochemicals against pathogenic bacteria","authors":"T. Manuroop, K Bharavi, A. Jahan, M Alpharaj, P. A. Kumar, G. S. Rao","doi":"10.22271/tpi.2020.v9.i3b.4448","DOIUrl":"https://doi.org/10.22271/tpi.2020.v9.i3b.4448","url":null,"abstract":"Enrofloxacin in combination with phytochemicals such as Curcumin (CUR), Piperin (PIP), Cinnamic acid (CIA), Caffeic acid (CAA) and Syringic acid (SYA) exhibits notable synergism against pathogenic bacteria. Chitosan-alginate encapsulated microspheres containing enrofloxacin and phytochemicals prepared and evaluated for their synergistic effect and reduction in individual agent’s disadvantages. CSALG microspheres were prepared by impregnating enrofloxacin (CS-ALG-EN) alone and in combination with respective phytochemicals such as CS-ALG-EN-CUR, CS-ALG-EN-PIP, CS-ALG-EN-CIA, CSALG-EN-CAA & CS-ALG-EN-SYA and evaluated for shape, size, loading efficacy, release kinetics of enrofloxacin and MIC of enrofloxacin along with various phytochemicals against MTCC and clinical isolate bacteria. Microspheres were spherical. When combined with phytochemicals the enrofloxacin loading efficacy decreased variably with respective phytochemicals. The % cumulative release of enrofloxacin from all microspheres was maximum at pH 1.2 and further increased at pH 6.8. CAA and SYA improved the release and CIA, CUR and PIP decreased the release of enrofloxacin from respective microspheres compared to CS-ALG-EN. The dissolution efficacy increased by addition of SYA, CAA while PIP, CIA and CUR decreased. The mean dissolution time is same in PIP, SYA, CAA while CIA showed lowest and CUR highest when compared with enrofloxacin alone loaded microspheres. The release of enrofloxacin followed korsmeyer-peppas model by following Fickian diffusion/Quasi-Fickian diffusion from spheres. The MIC of enrofloxacin significantly lowered in combination with CUR, PIP, CIA, CAA on both MTCC and clinical isolates of pathogenic bacteria. In conclusion chitosan-alginate encapsulation improved the bioavailability of enrofloxacin and phytochemicals and combination showed synergistic antibacterial effect.","PeriodicalId":23030,"journal":{"name":"The Pharma Innovation Journal","volume":"8 1","pages":"70-76"},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84887124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-01DOI: 10.22271/tpi.2020.v9.i3m.4551
Ajit Kumar
{"title":"Significance of va mycorrhiza and its response under varied soil conditions: An overview","authors":"Ajit Kumar","doi":"10.22271/tpi.2020.v9.i3m.4551","DOIUrl":"https://doi.org/10.22271/tpi.2020.v9.i3m.4551","url":null,"abstract":"","PeriodicalId":23030,"journal":{"name":"The Pharma Innovation Journal","volume":"60 2-3","pages":"737-740"},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91500986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-01DOI: 10.22271/tpi.2020.v9.i2g.4396
Deepak, Dinesh Gulia, Jitender Yadav, S. Deswal, J. Kadian
Livestock plays an important role in Indian rural economy and is a prime agricultural subsector. Endoparasites infections in domestic animals cause huge economical losses to the farmers. Nematodes like Strongyloides spp. are obligate gastrointestinal parasites of vertebrates. The larvated eggs of the Strongyloides spp. are expelled in the faeces of the host animals. The current study describes the clinical signs, diagnosis via microscopic faecal examination and medicinal management of Strogyloides spp. infection in Murrah calves. The infected calves were successfully treated with Piperazine and Levamisole.
{"title":"Medicinal management of Strongyloidiasis in murrah calves","authors":"Deepak, Dinesh Gulia, Jitender Yadav, S. Deswal, J. Kadian","doi":"10.22271/tpi.2020.v9.i2g.4396","DOIUrl":"https://doi.org/10.22271/tpi.2020.v9.i2g.4396","url":null,"abstract":"Livestock plays an important role in Indian rural economy and is a prime agricultural subsector. Endoparasites infections in domestic animals cause huge economical losses to the farmers. Nematodes like Strongyloides spp. are obligate gastrointestinal parasites of vertebrates. The larvated eggs of the Strongyloides spp. are expelled in the faeces of the host animals. The current study describes the clinical signs, diagnosis via microscopic faecal examination and medicinal management of Strogyloides spp. infection in Murrah calves. The infected calves were successfully treated with Piperazine and Levamisole.","PeriodicalId":23030,"journal":{"name":"The Pharma Innovation Journal","volume":"92 1","pages":"346-347"},"PeriodicalIF":0.0,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91328952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-01DOI: 10.22271/tpi.2020.v9.i2b.4345
K. Jeevan, Suguna Rao, M. Satyanarayana, G. Leena, Byregowda Sm, P. Krishnamoorthy
In the present study, hepatic tumors were chemically induced in male Wistar Albino rats with Diethylnitrosamine. Hepatic ultrasonography was performed to detect the development of hepatic tumors clinically at the end of treatment period. In sonography, the presence of tumor nodules was appreciated as the indistinct hepatic borders and circumscribed varied echogenicity with distinct hyperechoic borders in the affected portion of liver in contrast to the liver of negative control rats which showed distinct borders, homogenous parenchyma and medium echogenicity.
{"title":"Ultrasonographic detection of hepatic tumors in experimental model of chemical induced hepatocarcinogenesis in Wistar Albino rats","authors":"K. Jeevan, Suguna Rao, M. Satyanarayana, G. Leena, Byregowda Sm, P. Krishnamoorthy","doi":"10.22271/tpi.2020.v9.i2b.4345","DOIUrl":"https://doi.org/10.22271/tpi.2020.v9.i2b.4345","url":null,"abstract":"In the present study, hepatic tumors were chemically induced in male Wistar Albino rats with Diethylnitrosamine. Hepatic ultrasonography was performed to detect the development of hepatic tumors clinically at the end of treatment period. In sonography, the presence of tumor nodules was appreciated as the indistinct hepatic borders and circumscribed varied echogenicity with distinct hyperechoic borders in the affected portion of liver in contrast to the liver of negative control rats which showed distinct borders, homogenous parenchyma and medium echogenicity.","PeriodicalId":23030,"journal":{"name":"The Pharma Innovation Journal","volume":"49 1","pages":"52-54"},"PeriodicalIF":0.0,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76371908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.22271/TPI.2020.V9.I12SA.5393
K. JaganMohanReddy
The current study was undertaken to evaluate the clinical efficacy of Supracondylar femur plate for repair of supracondylar femur fractures in cats. Out of the clinical cases presented, 1% of cases in cats correspond to fractures, in which 25% correspond to supracondylar femur fractures. This study was conducted on six cats with age ranging from 4-11months, in 5 female and 1 male cat presented to the Department of Veterinary Surgery and Radiology at the College of Veterinary Science, Hyderabad with the chief complaint of falling from an height. These six cases of fractures were diagnosed by clinical signs, orthopaedic examination and survey radiography. The symptoms observed in the cats presented for treatment were pain on manipulation, abnormal angulation and lameness immediately after trauma, swelling, non-weight bearing, dangling of the limb and crepitation at the fracture site. Pre-operative radiographic examination in plain orthogonal views, i.e., cranio-caudal and medio-lateral radiographs revealed supracondylar fractures in all cats. These fractures were stabilized with 2.0 mm supracondylar femur plate 1 in right femur and 5 in left femur. Good implant stability throughout the treatment period without any complications was achieved all cats. In all six cats in the present study showed partial weight bearing on the 1st post- operative day. All cats showed normal weight bearing at rest, the weight bearing of the affected limb while walking from 2nd Post-operative day. All cats achieved complete weight bearing by 15th post-operative day. i.e. from Grade V to Grade I. The radiographs obtained on 30th day Post-operative day revealed proper apposition, decreased fracture gap, appearance of progressive bridging callus with adequate radio-density in all 6 cats, and on 60th day revealed continuity of complete corticomedullary cavity and compete bone formation without any complications with fast healing. Based on present study, it was concluded that supracondylar femur plate was successful in the treatment of supracondylar femur fractures in cats.
{"title":"Clinical study on the use of supracondylar femur plate (J plate/ hockey stick plate) for repair of supracondylar femur fractures in cats","authors":"K. JaganMohanReddy","doi":"10.22271/TPI.2020.V9.I12SA.5393","DOIUrl":"https://doi.org/10.22271/TPI.2020.V9.I12SA.5393","url":null,"abstract":"The current study was undertaken to evaluate the clinical efficacy of Supracondylar femur plate for repair of supracondylar femur fractures in cats. Out of the clinical cases presented, 1% of cases in cats correspond to fractures, in which 25% correspond to supracondylar femur fractures. This study was conducted on six cats with age ranging from 4-11months, in 5 female and 1 male cat presented to the Department of Veterinary Surgery and Radiology at the College of Veterinary Science, Hyderabad with the chief complaint of falling from an height. These six cases of fractures were diagnosed by clinical signs, orthopaedic examination and survey radiography. The symptoms observed in the cats presented for treatment were pain on manipulation, abnormal angulation and lameness immediately after trauma, swelling, non-weight bearing, dangling of the limb and crepitation at the fracture site. Pre-operative radiographic examination in plain orthogonal views, i.e., cranio-caudal and medio-lateral radiographs revealed supracondylar fractures in all cats. These fractures were stabilized with 2.0 mm supracondylar femur plate 1 in right femur and 5 in left femur. Good implant stability throughout the treatment period without any complications was achieved all cats. In all six cats in the present study showed partial weight bearing on the 1st post- operative day. All cats showed normal weight bearing at rest, the weight bearing of the affected limb while walking from 2nd Post-operative day. All cats achieved complete weight bearing by 15th post-operative day. i.e. from Grade V to Grade I. The radiographs obtained on 30th day Post-operative day revealed proper apposition, decreased fracture gap, appearance of progressive bridging callus with adequate radio-density in all 6 cats, and on 60th day revealed continuity of complete corticomedullary cavity and compete bone formation without any complications with fast healing. Based on present study, it was concluded that supracondylar femur plate was successful in the treatment of supracondylar femur fractures in cats.","PeriodicalId":23030,"journal":{"name":"The Pharma Innovation Journal","volume":"281 1","pages":"19-25"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86654476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.22271/tpi.2020.v9.i1g.4315
Hurmat, R. Shri, G. Bansal
Plants are the backbone of traditional systems of medicine and are also revered for their contribution to development of modern drugs. The therapeutic potential of plants depends on the quality and quantity of phytoconstituents present. Plant growth and the biosynthesis of plant metabolites are greatly influenced by biotic and abiotic factors. Scientific investigations have shown that various abiotic stresses including salt stress, flooding, drought, fertilization, shade, soil types etc. influence plant growth and formation of active constituents. Thus, optimization of abiotic stresses may help in increasing levels of plant metabolites and thus enhancing the bioactivity. The present review summarizes the importance of various abiotic stresses on plant growth and production of bioactive constituents. Literature shows that plants respond to abiotic stresses by modifying their morphology, physiology and phytochemical nature. Changes in plant growth and their bioactive metabolites are reported with alteration in abiotic stresses. This knowledge however is not translated to the fields during cultivation of valuable medicinal plants. From this review the authors conclude that, alteration of environmental factors during growth/cultivation of medicinal plants may ensure supply of plants with increased marker content which ensures better activity.
{"title":"Does abiotic stresses enhance the production of secondary metabolites? A review","authors":"Hurmat, R. Shri, G. Bansal","doi":"10.22271/tpi.2020.v9.i1g.4315","DOIUrl":"https://doi.org/10.22271/tpi.2020.v9.i1g.4315","url":null,"abstract":"Plants are the backbone of traditional systems of medicine and are also revered for their contribution to development of modern drugs. The therapeutic potential of plants depends on the quality and quantity of phytoconstituents present. Plant growth and the biosynthesis of plant metabolites are greatly influenced by biotic and abiotic factors. Scientific investigations have shown that various abiotic stresses including salt stress, flooding, drought, fertilization, shade, soil types etc. influence plant growth and formation of active constituents. Thus, optimization of abiotic stresses may help in increasing levels of plant metabolites and thus enhancing the bioactivity. The present review summarizes the importance of various abiotic stresses on plant growth and production of bioactive constituents. Literature shows that plants respond to abiotic stresses by modifying their morphology, physiology and phytochemical nature. Changes in plant growth and their bioactive metabolites are reported with alteration in abiotic stresses. This knowledge however is not translated to the fields during cultivation of valuable medicinal plants. From this review the authors conclude that, alteration of environmental factors during growth/cultivation of medicinal plants may ensure supply of plants with increased marker content which ensures better activity.","PeriodicalId":23030,"journal":{"name":"The Pharma Innovation Journal","volume":"112 1","pages":"412-422"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85648242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.22271/tpi.2020.v9.i9a.5178
V. Karande, Vaishnavi Sanjay Gagare, Sunidhi, R. Kotagiri, B. Ganguly
Mycotoxins are biologically active, toxic metabolites produced by toxigenic fungi mainly belonging to genus Aspergillus, Fusarium and Penicillium. They are ubiquitous in poultry feeds. Mix-02-Tox is a broad-spectrum mould inhibitor and mycotoxin binder, recommended for complete protection against multiple mycotoxins and for better production in poultry, cattle and pigs. A study was undertaken to evaluate the acute oral toxicity potential of Mix-02-Tox (M/s Ayurvet Limited, India) according to OECD 423 guidelines. Six (3 male and 3 female) Swiss albino mice were used for the study, where each animal served as its own control. Following the oral administration of the test substance, the animals were observed for the manifestation of toxic effects and mortality. No toxic effects or mortalities were observed till 14 days and Mix-02-Tox was found to be safe for oral use.
{"title":"Evaluation of acute oral toxicity of a polyherbal broad-spectrum mould inhibitor and mycotoxin binder","authors":"V. Karande, Vaishnavi Sanjay Gagare, Sunidhi, R. Kotagiri, B. Ganguly","doi":"10.22271/tpi.2020.v9.i9a.5178","DOIUrl":"https://doi.org/10.22271/tpi.2020.v9.i9a.5178","url":null,"abstract":"Mycotoxins are biologically active, toxic metabolites produced by toxigenic fungi mainly belonging to genus Aspergillus, Fusarium and Penicillium. They are ubiquitous in poultry feeds. Mix-02-Tox is a broad-spectrum mould inhibitor and mycotoxin binder, recommended for complete protection against multiple mycotoxins and for better production in poultry, cattle and pigs. A study was undertaken to evaluate the acute oral toxicity potential of Mix-02-Tox (M/s Ayurvet Limited, India) according to OECD 423 guidelines. Six (3 male and 3 female) Swiss albino mice were used for the study, where each animal served as its own control. Following the oral administration of the test substance, the animals were observed for the manifestation of toxic effects and mortality. No toxic effects or mortalities were observed till 14 days and Mix-02-Tox was found to be safe for oral use.","PeriodicalId":23030,"journal":{"name":"The Pharma Innovation Journal","volume":"52 1","pages":"49-51"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91279152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.22271/TPI.2020.V9.I11E.5359
M. Deepasri, V. Davamani, P. Kalaiselvi, S. Meena, M. Maheswari
An attempt has been made to use the treated paperboard mill effluent and well water with STCR recommended NPK, MLSS (Mixed Liquor Suspended Solids) and pressmud compost as nutrient source for cultivating Bhendi crop to assess their impact on yield and fruit quality. Application of MLSS, Pressmud compost had increased the available nutrients (N, P, K) and organic carbon content in the soil. The treatment combination of 50% STCR recommended NPK + 50% MLSS (Mixed Liquor Suspended Solids) + effluent irrigation performed better compared to other treatments. The experimental results revealed that yield of Bhendi under treated effluent irrigation was higher than well water irrigation. The yield increase was recorded as 31.20 percent over the control (100% STCR NPK + well water). The quality parameters viz., crude fibre and total protein were higher under effluent irrigation.
{"title":"Impact of treated papermill effluent on yield and quality of Bhendi","authors":"M. Deepasri, V. Davamani, P. Kalaiselvi, S. Meena, M. Maheswari","doi":"10.22271/TPI.2020.V9.I11E.5359","DOIUrl":"https://doi.org/10.22271/TPI.2020.V9.I11E.5359","url":null,"abstract":"An attempt has been made to use the treated paperboard mill effluent and well water with STCR recommended NPK, MLSS (Mixed Liquor Suspended Solids) and pressmud compost as nutrient source for cultivating Bhendi crop to assess their impact on yield and fruit quality. Application of MLSS, Pressmud compost had increased the available nutrients (N, P, K) and organic carbon content in the soil. The treatment combination of 50% STCR recommended NPK + 50% MLSS (Mixed Liquor Suspended Solids) + effluent irrigation performed better compared to other treatments. The experimental results revealed that yield of Bhendi under treated effluent irrigation was higher than well water irrigation. The yield increase was recorded as 31.20 percent over the control (100% STCR NPK + well water). The quality parameters viz., crude fibre and total protein were higher under effluent irrigation.","PeriodicalId":23030,"journal":{"name":"The Pharma Innovation Journal","volume":"10 1","pages":"298-300"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87404444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.38164/AJPER/9.3.2020.33-41
Pooja Chourasiya, R. Roy, Sarita Karole, S. Diwakar, Geeta Parkhe
A simple, sensitive, reliable and rapid reversed-phase high-performance liquid chromatographic (RPHPLC) method has been developed and validated for the determination of cloxacillin sodium in bulk and pharmaceutical dosage form. The chromatographic system consisted of waters (784), 515 binary Pump, data Ace with UV-Visible detector. Separation was achieved on the thermo C18 (250 x 4.60), 5 μ particle size column in isocratic mode at room temperature. The sample was introduced through an injector valve with a 20 μl, sample loop. 20mM KH2PO4 (pH-3.0 with OPA): Acetonitrile 20:80 (%, v/v), was used as mobile phase with flow rate of 1 ml/min. UV detection was performed at 224 nm. A calibration graph was plotted which showed a linearity range between 5-25μg/ml with the correlation coefficient of 0.999. The LOD was 0.095μg/ ml, while the LOQ was 0.271 μg/ml. Validation studies revealed the method is specific, rapid, reliable and reproducible. To study the validity of the method, recovery studies and repeatability studies were carried out using the same optimum conditions. The system suitability studies were also calculated which includes column efficiency, resolution, capacity factor and peak asymmetrical factor. Therefore the proposed method is reliable, rapid, precise and selective so may be used for the quantitative analysis of cloxacillin.
{"title":"Development and Validation of High Performance Liquid Chromatographic Method for the Determination of Cloxacillin Sodium in Bulk and Pharmaceutical Dosage Form","authors":"Pooja Chourasiya, R. Roy, Sarita Karole, S. Diwakar, Geeta Parkhe","doi":"10.38164/AJPER/9.3.2020.33-41","DOIUrl":"https://doi.org/10.38164/AJPER/9.3.2020.33-41","url":null,"abstract":"A simple, sensitive, reliable and rapid reversed-phase high-performance liquid chromatographic (RPHPLC) method has been developed and validated for the determination of cloxacillin sodium in bulk and pharmaceutical dosage form. The chromatographic system consisted of waters (784), 515 binary Pump, data Ace with UV-Visible detector. Separation was achieved on the thermo C18 (250 x 4.60), 5 μ particle size column in isocratic mode at room temperature. The sample was introduced through an injector valve with a 20 μl, sample loop. 20mM KH2PO4 (pH-3.0 with OPA): Acetonitrile 20:80 (%, v/v), was used as mobile phase with flow rate of 1 ml/min. UV detection was performed at 224 nm. A calibration graph was plotted which showed a linearity range between 5-25μg/ml with the correlation coefficient of 0.999. The LOD was 0.095μg/ ml, while the LOQ was 0.271 μg/ml. Validation studies revealed the method is specific, rapid, reliable and reproducible. To study the validity of the method, recovery studies and repeatability studies were carried out using the same optimum conditions. The system suitability studies were also calculated which includes column efficiency, resolution, capacity factor and peak asymmetrical factor. Therefore the proposed method is reliable, rapid, precise and selective so may be used for the quantitative analysis of cloxacillin.","PeriodicalId":23030,"journal":{"name":"The Pharma Innovation Journal","volume":"31 1","pages":"1024-1028"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90779577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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