Tissue & cell最新文献_第8页

Microarchitecture of the cavernous sinus walls: Clinical implications for pituitary adenomas 海绵窦壁的显微结构：垂体腺瘤的临床意义。

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell

Pub Date : 2026-04-01 Epub Date: 2025-11-12 DOI: 10.1016/j.tice.2025.103226

Khaled Abi Farraj , Vincent Jecko , Fanny Chasseloup , Paul Roblot , Dominique Liguoro , Clovis Adam , Fabien Guimiot , Peter Kamenicky , Etienne Lefevre

Background and purpose

The cavernous sinus (CS) is often invaded by pituitary adenomas, crossing the medial wall (MW), while the lateral wall (LW) is rarely breached. The structural differences between these walls remain poorly understood. This study investigated collagen subtypes distribution in human fetal and adult CS specimen to explore the basis for MW-specific invasion.

Methods

Twenty-two CS from eleven adult cadaveric human heads and two fetal specimens were dissected via transcranial and endoscopic approaches. Samples from the MW, LW, anterior wall, and diaphragm sellae were analyzed for collagen types I-IV staining by immunohistochemistry.

Results

All CS walls were identifiable in adult and fetal specimen. The MW and LW were composed of loose collagenous tissue; cranial nerves were present in the LW. In adults, type I collagen was weakly expressed in the MW and absent in the LW and fetal CS. Type II collagen appeared only in fetal cartilage. Type III collagen was present in the MW and partially in the LW of adults, and across both dural layers in fetuses. Type IV collagen was absent in adult dura but weakly present in fetal dura and strongly expressed in pituitary, perineurium, and carotid walls.

Conclusion

Collagen composition within the cavernous sinus undergoes marked changes with age, characterized by an increase in type I and III and a decline in type IV collagen. The structural differences, particularly between the MW and LW, may underlie the MW’s increased vulnerability to invasion by pituitary adenomas, potentially driven by MMP-dependent degradation pathways.

背景与目的：海绵窦（CS）常被垂体腺瘤侵犯，穿过内侧壁（MW），而侧壁（LW）很少被破坏。这些墙壁之间的结构差异仍然知之甚少。本研究通过对人胎儿和成人CS标本中胶原蛋白亚型分布的研究，探讨mw特异性侵袭的基础。方法：采用经颅和内窥镜方法对11例成人尸体头颅和2例胎儿头颅的22个CS进行解剖。采用免疫组织化学方法对腰、腰、前壁和膈鞍进行I-IV型胶原染色。结果：在成人和胎儿标本中均可识别所有CS壁。大、下丘脑由疏松的胶原组织组成；颅神经存在于左脑。在成人中，I型胶原蛋白在骨髓中弱表达，在腰周和胎儿CS中不存在。II型胶原蛋白仅出现在胎儿软骨中。III型胶原蛋白存在于成人的中脑膜，部分存在于下脑膜，胎儿的两层硬脑膜也有。IV型胶原蛋白在成人硬脑膜中不存在，但在胎儿硬脑膜中存在较弱，在垂体、神经膜和颈动脉壁中强烈表达。结论：海绵窦内胶原组成随年龄变化明显，表现为ⅰ型和ⅲ型胶原增加，ⅳ型胶原减少。结构上的差异，尤其是mmp和LW之间的差异，可能是mmp依赖性降解途径导致的mmp更易受到垂体腺瘤侵袭的原因。

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引用次数: 0

Oligosaccharides ameliorate insulin resistance and hepatic metabolism by promoting the leptin/POMC axis to accelerate short stature growth and development 低聚糖通过促进瘦素/POMC轴促进矮小身材的生长发育，改善胰岛素抵抗和肝脏代谢

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell

Pub Date : 2026-04-01 Epub Date: 2025-12-13 DOI: 10.1016/j.tice.2025.103277

Minhong Xu , Jin Zhou , Shuyao Zhang , Hongli Wang , Yongtao Zhang , Yinan Liu , Chengkuan Zhao

Background

Leptin and pro-opiomelanocortin (POMC) signaling critically regulates metabolism and growth. The potential of oligosaccharides to modulate this axis and its physiological impacts remains unclear.

Methods

Male wild-type (WT) and leptin-deficient (ob/ob) mice received daily oral oligosaccharides for 8 weeks. Lentiviral POMC overexpression (LV-POMC) or control (LV-NC) was administered to ob/ob mice. Metabolic function was assessed via glucose/insulin tolerance tests (GTT/ITT). Serum/tissue levels of leptin, POMC, metabolic markers, growth hormones, and bone regulators were quantified by enzyme-linked immunosorbent assay and Western blot. Femur length was recorded, and trabecular architecture was evaluated by hematoxylin-eosin staining.

Results

Oligosaccharides increased leptin and POMC levels in WT but not ob/ob mice. Oligosaccharides improved insulin sensitivity, hepatic metabolism (reduced triglycerides, aspartate aminotransferase, alanine aminotransferase, and free fatty acids), and bone growth (increased femur length, osteoprotegerin; decreased receptor activator of nuclear factor kappa-B ligand/cathepsin K) in WT mice. These benefits were absent in ob/ob mice. Crucially, POMC overexpression in ob/ob mice rescued metabolic dysfunction (improved GTT/ITT and normalized hepatic markers), restored growth hormone balance (reduced adrenocorticotropic hormone/cortisol and increased insulin-like growth factor 1), and reversed bone defects.

Conclusion

Oligosaccharides exert insulin-sensitizing, hepatoprotective, and growth-promoting effects via leptin-dependent POMC activation. POMC restoration rescues deficits in leptin deficiency, establishing the leptin/POMC axis as the essential mechanistic pathway.

背景：瘦素和促鸦片黑素皮质素（POMC）信号通路对代谢和生长具有关键调控作用。低聚糖调节这条轴的潜力及其生理影响尚不清楚。方法野生型（WT）和瘦素缺乏（ob/ob）小鼠每天口服低聚糖，持续8周。将慢病毒POMC过表达（LV-POMC）或对照（LV-NC）给予ob/ob小鼠。通过葡萄糖/胰岛素耐量试验（GTT/ITT）评估代谢功能。通过酶联免疫吸附法和Western blot定量测定血清/组织中瘦素、POMC、代谢标志物、生长激素和骨调节因子的水平。记录股骨长度，苏木精-伊红染色评价股骨小梁结构。结果单糖可提高WT小鼠的瘦素和POMC水平，但对ob/ob小鼠无显著影响。低聚糖改善了WT小鼠的胰岛素敏感性、肝脏代谢（减少甘油三酯、天冬氨酸转氨酶、丙氨酸转氨酶和游离脂肪酸）和骨生长（增加股骨长度、骨保护素；减少核因子κ b配体/组织蛋白酶K受体激活剂）。这些益处在ob/ob小鼠中不存在。至关重要的是，ob/ob小鼠的POMC过表达挽救了代谢功能障碍（改善GTT/ITT和正常化的肝脏标志物），恢复了生长激素平衡（促肾上腺皮质激素/皮质醇减少，胰岛素样生长因子1增加），并逆转了骨缺损。结论低聚糖通过瘦素依赖性的POMC激活发挥胰岛素增敏、保肝和促生长作用。POMC修复修复了瘦素缺乏症的缺陷，建立了瘦素/POMC轴作为重要的机制途径。

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引用次数: 0

Huang Qi Decoction inhibits differentiation of hepatic oval cells to cholangiocytes via the β-catenin pathway to ameliorate hepatic fibrosis 黄芪汤通过β-catenin途径抑制肝卵圆细胞向胆管细胞分化，改善肝纤维化

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell

Pub Date : 2026-04-01 Epub Date: 2025-12-16 DOI: 10.1016/j.tice.2025.103283

Zhi-dong Liu , Ke-pei Zhang , Dan-yang Wang , Xiao Zhang

Excessive cholangiocyte expansion promotes liver diseases progression. Wnt/β-catenin signaling is crucial in regulating cell differentiation. Our previous studies showed that Huang Qi Decoction (HQD) could ameliorate progression of cholestatic liver fibrosis (CLF) through inhibition of hepatic oval cells (HOCs) differentiation into cholangiocytes. However, the role of HQD in β-catenin signaling in cholangiocyte differentiation remains unclear. In this study, bile duct ligation (BDL) model was established in rats for in vivo experiment and hepatic oval cell line (WB-F344) via stimulation with sodium butyrate for in vitro experiment. Histology analysis showed that HQD ameliorated BDL-induced hepatic fibrosis (HF) with the reduction of alpha smooth muscle actin and collagen I expression. HQD inhibited CK-19 and EpCam protein expression and decreased hydroxyproline level in BDL rat liver. HQD inhibited Cyclin D1 and c-myc mRNA and protein expression levels and blocked β-catenin nuclear translocation in liver. Moreover, HQD inhibited Wnt3a expression in BDL rat liver. In vitro results showed that HQD inhibited differentiation of WB‑F344 cells into cholangiocyte with a decreased CK-19, Cyclin D1 and c-myc mRNA and protein expression. HQD blocked β-catenin nuclear translocation during hepatic oval cell differentiation to cholangiocytes in WB-F344 cells. In addition, overexpression of β-catenin promoted hepatic oval cell differentiation into cholangiocytes and abolished the inhibitory effect of HQD. Taken together, this study has highlighted that HQD could prevent CLF by inhibiting differentiation of HOCs into cholangiocytes via the β-catenin signaling pathway, which suggests that β-catenin may be considered as a potential target for HQD during CLF treatment.

胆管细胞过度扩张促进肝脏疾病的进展。Wnt/β-catenin信号在调节细胞分化中起着至关重要的作用。我们前期研究发现，黄芪汤通过抑制肝卵圆细胞向胆管细胞的分化，改善胆汁淤积性肝纤维化（CLF）的进展。然而，HQD在β-catenin信号在胆管细胞分化中的作用尚不清楚。本研究建立大鼠胆管结扎（BDL）模型进行体内实验，并通过丁酸钠刺激肝卵圆细胞株WB-F344进行体外实验。组织学分析显示，HQD通过降低α -平滑肌肌动蛋白和I型胶原的表达，改善bdl诱导的肝纤维化（HF）。HQD抑制BDL大鼠肝脏CK-19和EpCam蛋白表达，降低羟脯氨酸水平。HQD抑制肝脏细胞周期蛋白D1和c-myc mRNA和蛋白表达水平，阻断β-catenin核易位。此外，HQD可抑制BDL大鼠肝脏中Wnt3a的表达。体外结果显示，HQD抑制WB‑F344细胞向胆管细胞的分化，降低CK-19、Cyclin D1和c-myc mRNA和蛋白的表达。HQD阻断WB-F344细胞肝卵圆细胞向胆管细胞分化过程中β-catenin核易位。此外，β-catenin过表达可促进肝卵圆细胞向胆管细胞分化，消除HQD的抑制作用。综上所述，本研究强调了HQD可以通过β-catenin信号通路抑制hoc向胆管细胞的分化来预防CLF，这表明β-catenin可能被认为是CLF治疗过程中HQD的潜在靶点。

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引用次数: 0

Distribution of PGP 9.5- and nNOS-containing nerve fibers in the temporomandibular joint disk attachments of the elderly human 老年人颞下颌关节盘附着物中PGP 9.5和nnos神经纤维的分布。

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell

Pub Date : 2026-04-01 Epub Date: 2025-12-16 DOI: 10.1016/j.tice.2025.103282

Tadasu Sato , Hirotaka Ishigaki , Takehiro Yajima , Daisuke Tachiya , Hiroyuki Ichikawa

Innervation of the human temporomandibular joint (TMJ) disk and its surrounding structures posterior vascular plexus portion was investigated by immunohistochemistry for protein gene product 9.5 (PGP 9.5) and neuronal nitric oxide synthase (nNOS). The TMJ disk was free of PGP 9.5- and nNOS-immunoreactive (-ir) nerve fibers. In the TMJ disk attachments and posterior vascular plexus portion, however, PGP 9.5- and nNOS-immunoreactivity was detected. PGP 9.5-ir nerve fibers within nerve bundles were common in these regions. Perivascular PGP 9.5-ir nerve fibers were common in the attachments and posterior vascular plexus portion but not in the posterior attachment inferior. Isolated PGP 9.5-ir nerve fibers were also scattered throughout the TMJ attachments but not in the posterior vascular plexus portion. There was no significant difference about the density of PGP 9.5-ir nerve fibers among all of the attachments and posterior vascular plexus portion. NOS-ir nerve fibers within nerve bundles were common in the anterior and posterior attachments inferior, and the posterior vascular plexus portion. Perivascular nNOS-ir nerve fibers in the anterior attachment inferior were more abundant than in other regions. Isolated nNOS-ir nerve fibers were common in the anterior attachment inferior but not in other regions. Fine isolated nerve fibers with varicose appearance occasionally had small swellings in their terminals. Thick isolated nerve fibers rarely showed unencapsulated corpuscular endings with multiple large and spindle-shaped swellings. The density of nNOS-ir nerve fibers in the anterior attachment inferior was significantly higher than in the posterior attachment superior. In addition, the double immunofluorescence method demonstrated that vascular and isolated nNOS-ir nerve fibers were also immunoreactive for PGP 9.5 in the anterior attachment inferior. The present study suggests that nNOS-ir nerve fibers with a parasympathetic nature can control blood flow mainly in the anterior attachment inferior and slightly in other attachments and the posterior vascular plexus portion. nNOS-ir nerve fibers with a sensory nature may be associated with nociceptive transmission in the TMJ disk attachments.

采用免疫组化方法对人颞下颌关节（TMJ）盘及其周围结构后血管丛部分的神经支配进行了蛋白基因产物9.5 （PGP 9.5）和神经元型一氧化氮合酶（nNOS）的检测。颞下颌关节盘无PGP 9.5和nnos免疫反应（-ir）神经纤维。然而，在TMJ椎间盘附着体和后血管丛部分，检测到PGP 9.5和nnos免疫反应性。神经束内PGP 9.5-ir神经纤维在这些区域普遍存在。血管周围PGP 9.5-ir神经纤维常见于附着物和后血管丛部分，而不见于后附着物下方。离体PGP 9.5-ir神经纤维也分布在TMJ附着物中，但未分布在后血管丛部分。PGP - 9.5-ir神经纤维密度在各附着体及后血管丛部分间无显著差异。神经束内NOS-ir神经纤维常见于前、后附着体、下、后血管丛部分。前附着下血管周围nNOS-ir神经纤维较其他区域丰富。分离的nNOS-ir神经纤维在前附着下常见，而在其他区域不常见。有静脉曲张表现的细分离神经纤维偶尔在其末端有小的肿胀。粗的分离神经纤维很少有未包封的小体末梢，并有多个大的梭形肿胀。前附着下方的nNOS-ir神经纤维密度明显高于后附着上方。此外，双免疫荧光法显示前附着下的血管和离体nNOS-ir神经纤维对PGP 9.5也有免疫反应。本研究表明，具有副交感神经性质的nNOS-ir神经纤维主要控制前附著、下附著和后血管丛部分的血流，其他附著和后血管丛部分的血流控制较少。具有感觉性质的nNOS-ir神经纤维可能与颞下颌关节盘附着物的伤害性传递有关。

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引用次数: 0

P2Y2 receptor as a favorable predictor of gastric cancer P2Y2受体作为胃癌的有利预测因子。

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell

Pub Date : 2026-04-01 Epub Date: 2025-12-16 DOI: 10.1016/j.tice.2025.103278

Yu-qing Wu , Wen-long Wang

Background

Gastric cancer (GC) prevention and treatment have always been a difficult problem to solve. Therefore, mining the molecular genes related to the progression of GC and predicting the progression of GC has important clinical significance. Therefore, this study investigated whether the P2Y2 receptor (P2Y2R) has a certain effect on GC.

Methods

The correlation data of P2Y2R and GC tissues from public databases was collected, and the relationship between P2Y2R and the survival and prognosis of GC patients was analyzed. Moreover, the expression of P2Y2R in GC cells AGS, MGC803, HGC27 and normal GES-1 was detected by Western-blotting. Cell scratch, Transwell invasion and YF phalloidin assays were used to investigate the effects of P2Y2R on migration and invasion of GC cells.

Results

P2Y2R was highly expressed in GC tissues and was negatively correlated with poor survival and prognosis of patients with GC. Activation of the P2Y2R by UTP promoted the migration and invasion of GC cells. However, the P2Y2R-specific antagonist AR-C118925XX inhibited the migration and invasion of GC cells. In addition, P2Y2R activation enhanced cytoskeletal stress changes in GC cells and promoted GC motility, while inhibition of its activity yielded the opposite effect. In addition, activation of P2Y2R increased the expression levels of p-PKC, p-Src, and p-ERK1/2, while AR-C118925XX treatment significantly decreased the expression levels of p-PKC, p-Src, and p-ERK1/2.

Conclusion

High expression of P2Y2R is negatively correlated with survival and prognosis of GC patients. P2Y2R activation promotes GC progression may be related to PKC/Src and ERK signaling, indicating that P2Y2R may serve as a new molecular target for GC prevention and treatment.

背景：胃癌的防治一直是一个难以解决的问题。因此，挖掘与胃癌进展相关的分子基因，预测胃癌的进展具有重要的临床意义。因此，本研究探讨P2Y2受体（P2Y2R）是否对GC有一定影响。方法：收集公共数据库中P2Y2R与胃癌组织的相关数据，分析P2Y2R与胃癌患者生存及预后的关系。Western-blotting检测GC细胞AGS、MGC803、HGC27和正常GES-1中P2Y2R的表达。采用细胞划痕法、Transwell侵袭法和YF phalloidin法研究P2Y2R对胃癌细胞迁移和侵袭的影响。结果：P2Y2R在胃癌组织中高表达，与胃癌患者生存不良及预后呈负相关。UTP激活P2Y2R可促进GC细胞的迁移和侵袭。然而，p2y2r特异性拮抗剂AR-C118925XX抑制了GC细胞的迁移和侵袭。此外，P2Y2R的激活增强了GC细胞的细胞骨架应激变化，促进了GC运动，而抑制其活性则产生相反的效果。此外，P2Y2R的激活增加了p-PKC、p-Src和p-ERK1/2的表达水平，而AR-C118925XX处理显著降低了p-PKC、p-Src和p-ERK1/2的表达水平。结论：P2Y2R高表达与胃癌患者的生存和预后呈负相关。P2Y2R激活促进GC进展可能与PKC/Src和ERK信号通路有关，提示P2Y2R可能作为防治GC的新分子靶点。

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引用次数: 0

Corrigendum to “Targeting YBX1: A novel therapeutic strategy for gastric cancer through regulation of cellular senescence and mTOR signaling” [Tissue and Cell, 97 (2025) 103089] “靶向YBX1：通过调节细胞衰老和mTOR信号传导治疗胃癌的新策略”[组织和细胞，97(2025)103089]。

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell

Pub Date : 2026-04-01 Epub Date: 2026-01-15 DOI: 10.1016/j.tice.2026.103328

Wenze Zhang , Yanjuan Jia , Anqi Wang , Rui Guo , Zhuomin Fu , Wanxia Wang

引用次数: 0

Hepatoprotective effect of chitosan-vitamin C based hydrogel on alpha-Naphthylisothiocyanate-induced cholestasis in infant rats 壳聚糖-维生素C水凝胶对α -萘基异硫氰酸酯诱导的幼鼠胆汁淤积的肝保护作用。

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell

Pub Date : 2026-04-01 Epub Date: 2025-12-31 DOI: 10.1016/j.tice.2025.103307

Sarah Ali Qutb , Dalia Y. Saad , Ayman Saber Mohamed

Background

Infant cholestasis is characterized by the buildup of biliary substances in blood and extrahepatic tissues because of impaired canalicular biliary flow, which harm the liver and bile ducts. Vitamin C (Vit C) is antioxidant which has demonstrated encouraging outcomes in the treatment of different liver disorders, including fibrosis.

Aim

The present study seeks to assess the synergistic protective effect of chitosan and vitamin C based hydrogel (CSVC hydrogel) on alpha-Naphthylisothiocyanate (ANIT)-induced cholestasis in infant rats.

Method

Thirty male Wister rats were divided into five groups: control, ANIT, and cholestatic rats treated with chitosan hydrogel (60 mg/kg body weight, orally), Vit C (40 mg/kg body weight, orally), and CSVC hydrogel (60 mg/kg body weight, orally). Liver functions, oxidative stress, inflammatory, and apoptotic markers were investigated.

Results

CSVC hydrogel had remarkable antioxidant and anti-inflammatory properties in vitro. The administration of CSVC hydrogel significantly improved liver function, evidenced by decreased hepatic enzyme activity such as AST (28.11 ± 0.83^###), ALT (16.56 ± 0.95^###), ALP (126.49 ± 3.06^###), GGT (0.11 ± 0.02^###), total bilirubin (1.71 ± 0.05^###) and a rise in albumin levels to 3.11 ± 0.10^###. Moreover, CSVC hydrogel resulted in an increase in reduced glutathione and the activity of antioxidant enzymes (GST, SOD, GPx, and catalase), while simultaneously decreasing the production of malondialdehyde and nitric oxide. CSVC hydrogel decreased DNA damage in cholestatic rats and partially corrected the abnormal structure of hepatic tissues in ANIT rats. CSVC hydrogel decreased inflammation by decreasing concentrations of interleukin 6 (IL-6) and interleukin 1β (IL-1β) while enhancing the antiapoptotic protein BCL2. The immunohistochemistry analysis revealed a reduction in inflammation and apoptosis, through the decreased production of tumor necrosis factor alpha (TNF-α) and Caspase 3.

Conclusion

CSVC hydrogel mitigated liver fibrosis via its anti-inflammatory, anti-apoptotic, antioxidant properties, and its DNA protective effects.

背景：婴儿胆汁淤积症的特点是由于小管胆道流动受损，导致血液和肝外组织中胆道物质积聚，从而损害肝脏和胆管。维生素C （Vit C）是一种抗氧化剂，在治疗包括纤维化在内的各种肝脏疾病方面显示出令人鼓舞的结果。目的：探讨壳聚糖和维生素C基水凝胶（CSVC水凝胶）对α -萘基异硫氰酸酯（ANIT）诱导的幼鼠胆汁淤积的协同保护作用。方法：将30只雄性Wister大鼠分为5组：对照组、ANIT组和胆汁淤积大鼠，分别给予壳聚糖水凝胶（60 mg/kg体重，口服）、Vit C（40 mg/kg体重，口服）和CSVC水凝胶（60 mg/kg体重，口服）。研究肝功能、氧化应激、炎症和凋亡标志物。结果：CSVC水凝胶具有明显的体外抗氧化和抗炎作用。管理CSVC水凝胶明显改善肝功能,证明通过减少肝酶活性,如AST(28.11 ± 0.83 # # #)、ALT(16.56 ± 0.95 # # #),高山(126.49 ± 3.06 # # #)、GGT(0.11 ± 0.02 # # #)、总胆红素(1.71 ± 0.05 # # #)和白蛋白水平上升到3.11 ± 0.10 # # #。此外，CSVC水凝胶导致还原性谷胱甘肽和抗氧化酶（GST、SOD、GPx和过氧化氢酶）活性的增加，同时减少丙二醛和一氧化氮的产生。CSVC水凝胶能减轻胆汁淤积大鼠DNA损伤，部分纠正ANIT大鼠肝脏组织结构异常。CSVC水凝胶通过降低白细胞介素6 （IL-6）和白细胞介素1β (IL-1β)浓度，增强抗凋亡蛋白BCL2来减轻炎症反应。免疫组化分析显示，通过降低肿瘤坏死因子α (TNF-α)和Caspase 3的产生，炎症和细胞凋亡减少。结论：CSVC水凝胶通过抗炎、抗凋亡、抗氧化及DNA保护作用减轻肝纤维化。

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引用次数: 0

Analysis of WNT5A as a key regulator of intramuscular fat deposition in muscle-adipocyte co-cultures 分析WNT5A作为肌肉-脂肪细胞共培养中肌内脂肪沉积的关键调节因子

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell

Pub Date : 2026-04-01 Epub Date: 2025-11-24 DOI: 10.1016/j.tice.2025.103245

Hongfang Gong , Yaping Song , Dongmei Yang , Chao Jiang , Ruopu Jiao , Yilun Ma , Sayed Haidar Abbas Raza , Jiupan Zhang , Dawei Wei

The coordinated development of skeletal muscle and intramuscular adipose tissue in animals essentially determines meat yield and quality, a process co-regulated by multiple genes. Using a co-culture model of bovine skeletal muscle cells (SMCs) and intramuscular adipocytes (IMAs), integrated with transcriptomic sequencing and bioinformatic analyses, key candidate genes coregulating muscle development and fat deposition were identified. Three potential coregulators—WNT5A, APOE, and BDKRB2—were selected. Protein-protein interaction (PPI) network analysis, along with tissue and cellular expression profiling, indicates that WNT5A potentially interacts with key protein markers of adipogenesis and myogenesis. Furthermore, it is highly expressed in both adipose and muscle tissues. Pathway enrichment analysis revealed significant enrichment of WNT5A in the Wnt signaling pathway. These findings suggest that WNT5A plays a dual regulatory role in the development of both skeletal muscle and intramuscular fat (IMF). This finding lays a solid theoretical foundation for deciphering the molecular mechanisms of muscle-fat deposition in beef cattle and for improving meat quality.

动物骨骼肌和肌内脂肪组织的协调发育从本质上决定了肉的产量和品质，这一过程由多个基因共同调控。利用牛骨骼肌细胞（SMCs）和肌内脂肪细胞（IMAs）共培养模型，结合转录组测序和生物信息学分析，确定了协同调节肌肉发育和脂肪沉积的关键候选基因。三个潜在的协同调节因子- wnt5a， APOE和bdkrb2 -被选中。蛋白-蛋白相互作用（PPI）网络分析以及组织和细胞表达谱表明，WNT5A可能与脂肪形成和肌肉形成的关键蛋白标志物相互作用。此外，它在脂肪和肌肉组织中都高度表达。通路富集分析显示Wnt信号通路中WNT5A显著富集。这些发现表明WNT5A在骨骼肌和肌内脂肪（IMF）的发育中起双重调节作用。这一发现为破解肉牛肌肉脂肪沉积的分子机制，提高肉质品质奠定了坚实的理论基础。

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引用次数: 0

Oroxindin promotes angiogenesis in pressure ulcers through activating PI3K/AKT signaling pathway by PTEN suppression Oroxindin通过抑制PTEN激活PI3K/AKT信号通路促进压疮血管生成。

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell

Pub Date : 2026-04-01 Epub Date: 2025-12-21 DOI: 10.1016/j.tice.2025.103291

Tengyan Zhu , Shuzhen Fang , Xiaoqin Shang

Pressure ulcer (PU) is defined as localized tissue damage caused by prolonged pressure on any part of the body. Oroxindin (Oro) exhibits significant anti-inflammatory and anti-cancer effects. This study aimed to investigate the effect and underlying mechanism of Oro on PU. Angiogenesis was detected by CD31 immunohistochemistry and CD31/α-SMA immunofluorescence staining in vivo. Cell scratch assay, Transwell assay, and tube formation assay were performed to assess cell migration and angiogenesis in vitro. Phospho-kinase array was used to identify the pathway in Oro-treated human umbilical vein endothelial cells (HUVECs), and the mechanism was investigated by Western blot, luciferase reporter gene assay, biolayer interferometry assay, and molecular docking. Results showed that Oro accelerated wound healing and angiogenesis in the PU mouse model. Moreover, Oro promoted cell migration and tube formation in HUVECs. In addition, Oro activated the PI3K/AKT signaling pathway through the suppression of PTEN. PTEN overexpression reversed the effects of Oro on cell migration and tube formation in HUVECs. In conclusion, we demonstrated that Oro promoted PU wound healing in vivo and facilitated angiogenesis in HUVECs through activation of the PI3K/AKT signaling pathway via PTEN suppression, indicating the potential of Oro as an effective treatment for PU.

压疮（PU）被定义为由于长期压迫身体任何部位而引起的局部组织损伤。Oroxindin （Oro）具有显著的抗炎和抗癌作用。本研究旨在探讨Oro对PU的影响及其机制。采用CD31免疫组化和CD31/α-SMA免疫荧光染色检测血管新生。采用细胞划痕实验、Transwell实验和试管形成实验评估细胞在体外的迁移和血管生成。采用磷酸激酶阵列技术在oro处理的人脐静脉内皮细胞（HUVECs）中鉴定该通路，并通过Western blot、荧光素酶报告基因检测、生物层干涉检测和分子对接等方法研究其作用机制。结果表明，Oro能促进PU小鼠模型的创面愈合和血管生成。此外，Oro还能促进huvec细胞的迁移和管的形成。此外，Oro通过抑制PTEN激活PI3K/AKT信号通路。PTEN过表达逆转了Oro对huvec细胞迁移和管形成的影响。综上所述，我们证明Oro通过抑制PTEN激活PI3K/AKT信号通路，在体内促进PU伤口愈合，促进HUVECs血管生成，表明Oro可能是一种有效的PU治疗方法。

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引用次数: 0

Ultrastructural configuration of porcine liver preserved by machine perfusion versus static cold storage donated cardiac after death 机器灌注保存的猪肝与静态冷藏的死后捐献心脏超微结构的比较

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell

Pub Date : 2026-04-01 Epub Date: 2025-12-05 DOI: 10.1016/j.tice.2025.103262

Hiroki Bochimoto , Nur Khatijah Mohd Zin , Yo Ishihara , Tetsuya Nakajo , Hiromichi Obara , Naoto Matsuno , Daisuke Kondoh

In liver transplantation, it is known that machine perfusion (MP) preservation of liver grafts donated after cardiac death is superior to static cold storage (SCS). However, hepatocyte ultrastructure appears to be normal after SCS, while MP induces significant ultrastructural changes of hepatocytes such as mitochondrial swelling. The differences between these ultrastructural findings and physiological/clinical findings should be evaluated in detail. Here, we examined porcine liver grafts after SCS 4°C or MP at 8°C by osmium-maceration scanning (OM-SEM) and transmission (TEM) electron microscopic analyses. The overall appearance of hepatocytes in the SCS group was relatively normal, while the cytoplasm in the MP group was abnormal with swollen mitochondria and heavily-foamy endoplasmic reticulum. However, bile canaliculi of the liver grafts after SCS were filled with disintegrated materials which represent the exfoliated microvilli, while those after MP had a normal microvilli structure. Liver sinusoidal endothelial cells (LSECs) after SCS showed a heavy cytopathic change accompanied by large vacuoles, whereas LSECs after MP had no or significantly smaller vacuoles that appeared to be autophagosomes. These findings indicated that functional ultrastructure of liver grafts was better preserved in MP condition than in SCS. This is contrary to the impression given by the overall image of hepatocytes, and thus ultrastructural evaluation focusing on specific functional structures like bile canaliculi and LSECs have advantages to of liver grafts. (222/250 words)

在肝移植中，已知心脏死亡后捐献的肝移植物的机器灌注（MP）保存优于静态冷储存（SCS）。然而，SCS后肝细胞超微结构似乎正常，而MP诱导肝细胞超微结构发生明显变化，如线粒体肿胀。应详细评估这些超微结构表现与生理/临床表现之间的差异。在这里，我们通过锇浸渍扫描（OM-SEM）和透射电镜（TEM）分析了4°C SCS或8°C MP后的猪肝移植物。SCS组肝细胞整体外观相对正常，而MP组细胞质异常，线粒体肿胀，内质网大量泡沫。然而，SCS后肝移植物的胆管内充满了崩解的物质，代表脱落的微绒毛，而MP后的肝移植物的微绒毛结构正常。肝窦内皮细胞（LSECs）在SCS后表现出严重的细胞病变，伴有大空泡，而MP后的LSECs没有或明显更小的空泡，似乎是自噬体。这些结果表明，与SCS相比，MP条件下肝移植的功能超微结构得到了更好的保存。这与肝细胞整体图像给人的印象相反，因此关注特定功能结构（如胆管和LSECs）的超微结构评估对肝移植具有优势。(222/250字)

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