B. Ozturk, H. Vatansev, Alpaslan Taner, S. Yılmaz, M. Dağlı, A. Unlu
Objective: Behcet's disease (BD) is a chronic, inflammatory, multisystem vasculitis and the etiology is not yet fully understood. Nitric Oxide (NO) is an important molecule for the vascular system which synthesised by the Nitric Oxide Synthase (NOS) enzyme. Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of NOS. Therefore, we aimed to investigate levels of the ADMA and its relation with exacerbations in BD patients. Methods: The subjects enrolled in this study were recruited from 34 patients with BD and 34 healthy controls. Serum levels of ADMA, arginine and citrulline were measured by HPLC with fluorescence detection. The ESR and CRP levels analyzed by routinely used methods. Results: In contrast to information in the literature, ADMA levels were found to be decreased in BD patients when compared to control. Inversely, Arginine and Citrulline levels were significantly increased in BD patients. Increased Arginine/ADMA ratios were found in the patient group. There was an inverse relation between ADMA and CRP levels. Arginine values were also correlated CRP and Citrulline levels. Conclusion: One of the reasons of high number of BD attacks in young age may be low levels of ADMA. Our study suggests that the reduced levels of ADMA in patients group may impose a possible preventive role to ADMA through decrease of NO-mediated inflammation and exacerbations of BD with aging.
{"title":"Asymmetric dimethylarginine levels in Behçet's Disease","authors":"B. Ozturk, H. Vatansev, Alpaslan Taner, S. Yılmaz, M. Dağlı, A. Unlu","doi":"10.5505/TJB.2014.74436","DOIUrl":"https://doi.org/10.5505/TJB.2014.74436","url":null,"abstract":"Objective: Behcet's disease (BD) is a chronic, inflammatory, multisystem vasculitis and the etiology is not yet fully understood. Nitric Oxide (NO) is an important molecule for the vascular system which synthesised by the Nitric Oxide Synthase (NOS) enzyme. Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of NOS. Therefore, we aimed to investigate levels of the ADMA and its relation with exacerbations in BD patients. Methods: The subjects enrolled in this study were recruited from 34 patients with BD and 34 healthy controls. Serum levels of ADMA, arginine and citrulline were measured by HPLC with fluorescence detection. The ESR and CRP levels analyzed by routinely used methods. Results: In contrast to information in the literature, ADMA levels were found to be decreased in BD patients when compared to control. Inversely, Arginine and Citrulline levels were significantly increased in BD patients. Increased Arginine/ADMA ratios were found in the patient group. There was an inverse relation between ADMA and CRP levels. Arginine values were also correlated CRP and Citrulline levels. Conclusion: One of the reasons of high number of BD attacks in young age may be low levels of ADMA. Our study suggests that the reduced levels of ADMA in patients group may impose a possible preventive role to ADMA through decrease of NO-mediated inflammation and exacerbations of BD with aging.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76681079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: In this study, the fatty acid compositions of 32 different almond (Prunus dulcis (Mill.) D.A. Webb) genotypes seeds that collected from South and South East Anatolia regions in Turkey were studied. Methods: For lipid extraction of almond genotypes Hara and Radin (1978) method were used. Fatty acids content were determined using gas chromatographic (GC) analysis. The datas were evalulated with SPSS 17.0 statistical program. Results: In the gas chromatographic analysis, palmitic acid (16:0), palmitoleic acid (16:1), stearic acid (18:0), oleic acid (18:1), linoleic acid (18:2), and linolenic acid (18:3) were determined to be 5.34%, 0.70%, 0.85%, 74.46%, 17.89%, and 0.75% respectively. Saturated fatty acids (SFA) 6.19%, unsaturated fatty acids (USFA) 93.81% and a rate of USFA/SFA of 15.40, monounsaturated fatty acids (MUFA) 75.16%, polyunsaturated fatty acids (PUFA) 18.65% and a MUFA/PUFA ratio of 4.32 were found. In the correlation analysis, the highest correlation coefficient (r=-0.988) was detected between oleic acid and linoleic acid. Relationships among almond samples were partially identified with the cluster analysis. Conclusion: In this study, the variations were found between almond genotypes collected from different locations in terms of fatty acids compositions. Besides, the almond genotypes that have high quality unsaturates fatty acids such as high oleic acids and low linoleik acids content
{"title":"Major fatty acids composition of 32 almond (Prunus dulcis (Mill.) D.A. Webb) genotypes distributed in East and Southeast of Anatolia","authors":"H. Karatay, A. Aslan","doi":"10.5505/TJB.2014.55477","DOIUrl":"https://doi.org/10.5505/TJB.2014.55477","url":null,"abstract":"Objective: In this study, the fatty acid compositions of 32 different almond (Prunus dulcis (Mill.) D.A. Webb) genotypes seeds that collected from South and South East Anatolia regions in Turkey were studied. Methods: For lipid extraction of almond genotypes Hara and Radin (1978) method were used. Fatty acids content were determined using gas chromatographic (GC) analysis. The datas were evalulated with SPSS 17.0 statistical program. Results: In the gas chromatographic analysis, palmitic acid (16:0), palmitoleic acid (16:1), stearic acid (18:0), oleic acid (18:1), linoleic acid (18:2), and linolenic acid (18:3) were determined to be 5.34%, 0.70%, 0.85%, 74.46%, 17.89%, and 0.75% respectively. Saturated fatty acids (SFA) 6.19%, unsaturated fatty acids (USFA) 93.81% and a rate of USFA/SFA of 15.40, monounsaturated fatty acids (MUFA) 75.16%, polyunsaturated fatty acids (PUFA) 18.65% and a MUFA/PUFA ratio of 4.32 were found. In the correlation analysis, the highest correlation coefficient (r=-0.988) was detected between oleic acid and linoleic acid. Relationships among almond samples were partially identified with the cluster analysis. Conclusion: In this study, the variations were found between almond genotypes collected from different locations in terms of fatty acids compositions. Besides, the almond genotypes that have high quality unsaturates fatty acids such as high oleic acids and low linoleik acids content","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84917798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Determınatıon Of The Medıan Values Of Trıple Test Screening Parametres İn Yozgat Regıon","authors":"Gokhan Dogukan Akarsu","doi":"10.5505/TJB.2014.25338","DOIUrl":"https://doi.org/10.5505/TJB.2014.25338","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85936231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim:To screen various yeast cultures and agro-industrial by-products and optimization of fermentation conditions for the microbial production of extracellular lipase under solid state fermentation technique. Material and Methods: Various yeast cultures including Candida lipolytica NRRL-Y-1095, Candida utilis NRRL-Y-900, Candida tropicalis NRRL-Y-1552, Saccharomyces cerevisiae IIB-1 were screened by culturing on agro-industrial by-products under batch culture using solid state fermentation in 250 mL Erlenmeyer flasks. The medium was supplemented with various nitrogen sources (both organic and inorganic) and metal ions. Results: Candida utilis NRRL-Y-900 showed the highest enzyme production on soybean meal. Various particle sizes of substrate and moistening agents were also optimized for the maximum lipase synthesis. The optimum temperature and pH for the accumulation of enz- yme by Candida utilis NRRL-Y-900 was 30oC and 6.5, respectively. The fermentation time of 60h was suitable for the maximum enzyme production by using 7.5% inoculum of 24h old yeast culture. The optimal medium composition consisted of 2% (w/v) meat extract, 0.4% (w/v) ammonium sulphate and 5mM Fe+2. The maximum extracellular lipase production was 3.96±0.09 U. Conclusion: The results obtained during the study are significant for, to our knowledge, it is the first report regarding the utilization of soybean meal by C. utilis NRRL-Y-900 to accu- mulate lipase under optimized conditions and solid state fermentation.
{"title":"Production of an extracellular lipase by Candida utilis NRRL-Y-900 using agro-industrial by-products","authors":"A. Rehman, Sunniya Rasool, H. Mukhtar, I. Haq","doi":"10.5505/TJB.2014.96977","DOIUrl":"https://doi.org/10.5505/TJB.2014.96977","url":null,"abstract":"Aim:To screen various yeast cultures and agro-industrial by-products and optimization of fermentation conditions for the microbial production of extracellular lipase under solid state fermentation technique. Material and Methods: Various yeast cultures including Candida lipolytica NRRL-Y-1095, Candida utilis NRRL-Y-900, Candida tropicalis NRRL-Y-1552, Saccharomyces cerevisiae IIB-1 were screened by culturing on agro-industrial by-products under batch culture using solid state fermentation in 250 mL Erlenmeyer flasks. The medium was supplemented with various nitrogen sources (both organic and inorganic) and metal ions. Results: Candida utilis NRRL-Y-900 showed the highest enzyme production on soybean meal. Various particle sizes of substrate and moistening agents were also optimized for the maximum lipase synthesis. The optimum temperature and pH for the accumulation of enz- yme by Candida utilis NRRL-Y-900 was 30oC and 6.5, respectively. The fermentation time of 60h was suitable for the maximum enzyme production by using 7.5% inoculum of 24h old yeast culture. The optimal medium composition consisted of 2% (w/v) meat extract, 0.4% (w/v) ammonium sulphate and 5mM Fe+2. The maximum extracellular lipase production was 3.96±0.09 U. Conclusion: The results obtained during the study are significant for, to our knowledge, it is the first report regarding the utilization of soybean meal by C. utilis NRRL-Y-900 to accu- mulate lipase under optimized conditions and solid state fermentation.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88248277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Econazole, an azole compound widely used as an antifungal drug, is currently investigated for additional therapeutic effects. In fact, the antitumoral properties of econa- zole have been recently demonstrated, both in vivo and at the cellular level. However, the precise mechanism of action behind its effects is still unclear. Aim: To examine the effect of econazole on intracellular Ca2+ signaling pathways in a human adenocarcinoma cell line.
{"title":"Effect of econazole on Ca2+ signaling in human colorectal adenocarcinoma cells","authors":"Celia Carrillo, M. M. Cavia, S. Alonso-Torre","doi":"10.5505/TJB.2013.08370","DOIUrl":"https://doi.org/10.5505/TJB.2013.08370","url":null,"abstract":"Introduction: Econazole, an azole compound widely used as an antifungal drug, is currently investigated for additional therapeutic effects. In fact, the antitumoral properties of econa- zole have been recently demonstrated, both in vivo and at the cellular level. However, the precise mechanism of action behind its effects is still unclear. Aim: To examine the effect of econazole on intracellular Ca2+ signaling pathways in a human adenocarcinoma cell line.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84428529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nabil Abdul, Aleem Sidek, Adyani Azizah, A. Halim, H. Kadir, Saad Tayyab
Aim: To study the conformational stability of commercial ficin (CF) in the presence of guanidine hydrochloride (GdnHCl), urea, ethanol or at acidic pH and compare it with that reported for the major ficin fraction (MFF) obtained by purification of CF. Methods: Far-UV and near-UV CD spectral signals, intrinsic fluorescence, acrylamide quenching and enzymatic activity were used to study the effects of chemical denaturants and acidic pH on CF. The data were analyzed using two-state hypothesis, if required. Results: GdnHCl produced complete loss of secondary and tertiary structures of the protein. Loss of all enzymatic activity was observed at 4 M GdnHCl. CF showed structural resistance against 9 M urea and 50 % ethanol. Significant differences in emission maximum, acrylamide quenching, denaturation transition and enzymatic activity were noted between CF and MFF treated with different denaturants. CF showed greater stability at acidic pH than MFF. Conclusion: We conclude that CF is more structurally resistant than MFF against chemical and acid denaturations.
目的:研究商品无花果素(CF)在盐酸胍(GdnHCl)、尿素、乙醇和酸性pH条件下的构象稳定性，并与报道的纯化商品无花果素的主要组分(MFF)的构象稳定性进行比较。利用远紫外和近紫外CD光谱信号、本征荧光、丙烯酰胺猝灭和酶活性研究化学变性剂和酸性pH对CF的影响，如果需要，使用双态假设对数据进行分析。结果:GdnHCl使该蛋白的二级和三级结构完全丧失。在4 M GdnHCl下观察到所有酶活性的丧失。CF对9 M尿素和50%乙醇具有结构抗性。不同变性剂处理的CF和MFF在最大排放量、丙烯酰胺猝灭、变性转变和酶活性方面存在显著差异。CF在酸性pH下的稳定性优于MFF。结论:CF在结构上比MFF更耐化学和酸变性。
{"title":"Structural stability of commercial ficin under different denaturing conditions","authors":"Nabil Abdul, Aleem Sidek, Adyani Azizah, A. Halim, H. Kadir, Saad Tayyab","doi":"10.5505/TJB.2013.33043","DOIUrl":"https://doi.org/10.5505/TJB.2013.33043","url":null,"abstract":"Aim: To study the conformational stability of commercial ficin (CF) in the presence of guanidine hydrochloride (GdnHCl), urea, ethanol or at acidic pH and compare it with that reported for the major ficin fraction (MFF) obtained by purification of CF. Methods: Far-UV and near-UV CD spectral signals, intrinsic fluorescence, acrylamide quenching and enzymatic activity were used to study the effects of chemical denaturants and acidic pH on CF. The data were analyzed using two-state hypothesis, if required. Results: GdnHCl produced complete loss of secondary and tertiary structures of the protein. Loss of all enzymatic activity was observed at 4 M GdnHCl. CF showed structural resistance against 9 M urea and 50 % ethanol. Significant differences in emission maximum, acrylamide quenching, denaturation transition and enzymatic activity were noted between CF and MFF treated with different denaturants. CF showed greater stability at acidic pH than MFF. Conclusion: We conclude that CF is more structurally resistant than MFF against chemical and acid denaturations.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90946532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Özmeriç, H. Burç, Nevres Hürriyet, Y. Baykal, T. Atay
Objectives: The routine laboratory parameters for detection of early infection could be increased after surgical trauma in endoprosthesis surgery. The aim of this study was to compare the early infective complication marker Procalcitonin with routine markers. Methods: Twenty patients with primary total hip prosthesis and 30 knee prosthesis were enrolled. The changes in procalcitonin, C-reactive protein levels, white blood cell count, and erythrocyte sedimentation rate were evaluated preoperatively, at postoperative first day, postoperative fifth day and on the day of discharge. Results: Procalcitonin values of patients who developed superficial infection were statistically high in comparison with uncomplicated patients at post-op Day 1 and Day 5 (p < 0.05). The level of C-reactive protein, white blood cell count, and erythrocyte sedimentation rate peaked on postoperative Day 1. These levels decreased by postoperative Day 5 and on the day of discharge but did not reach preoperative mean values. Conclusion: Procalcitonin is a more selective parameter to predict early infection status following total endoprosthetic surgery. When factors that cause an inflammatory response were eradicated, procalcitonin levels dropped more rapidly and followed a standard postoperative kinetic pathway.
{"title":"Is procalcitonin a more sensitive parameter than other acute phase reactants for early infection in arthroplasty","authors":"A. Özmeriç, H. Burç, Nevres Hürriyet, Y. Baykal, T. Atay","doi":"10.5505/TJB.2013.83584","DOIUrl":"https://doi.org/10.5505/TJB.2013.83584","url":null,"abstract":"Objectives: The routine laboratory parameters for detection of early infection could be increased after surgical trauma in endoprosthesis surgery. The aim of this study was to compare the early infective complication marker Procalcitonin with routine markers. Methods: Twenty patients with primary total hip prosthesis and 30 knee prosthesis were enrolled. The changes in procalcitonin, C-reactive protein levels, white blood cell count, and erythrocyte sedimentation rate were evaluated preoperatively, at postoperative first day, postoperative fifth day and on the day of discharge. Results: Procalcitonin values of patients who developed superficial infection were statistically high in comparison with uncomplicated patients at post-op Day 1 and Day 5 (p < 0.05). The level of C-reactive protein, white blood cell count, and erythrocyte sedimentation rate peaked on postoperative Day 1. These levels decreased by postoperative Day 5 and on the day of discharge but did not reach preoperative mean values. Conclusion: Procalcitonin is a more selective parameter to predict early infection status following total endoprosthetic surgery. When factors that cause an inflammatory response were eradicated, procalcitonin levels dropped more rapidly and followed a standard postoperative kinetic pathway.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73885484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Epigenetic modulation of gene expression by DNA promoter methylation may contribute to acquired resistance to chemotherapy in cancer cells. Decitabine (5-aza-2'- deoxycytidine), a demethylating agent, may act synergistically with standard chemotherapy regimens to activate epigenetically silenced genes. In the present in vitro study, it was investigated the effect of gene methylation level after treatment with decitabine and combination of decitabine with anthracycline-based therapeutics (5-fluorouracil plus epirubicine plus cyclophosphamide; FEC) on breast cancer cells (MCF-7 and MDA-MB-231). Methods: The effect of decitabine and its combination with FEC on different genes methylation level has been tested in MDA-MB-231 and MCF-7 human breast cancer cell lines. The effect of decitabine on the cell viability was assayed by MTT assay. Methylight real-time PCR and methylation specific PCR were carried out to determine the methylation status of certain genes: DAPK, TMS1, MGMT and the global methylation marker LINE-1. Results: The LINE-1 methylation status significantly decreased in both cell lines after treatment with the combination of decitabine with FEC. In MDA-MB-231 cells, methylation of the TMS1 and the MGMT gene promoter was significantly reduced by FEC plus decitabine while no effect was observed in MCF-7 cells. Conclusion: Anthracycline-based therapy regimens in combination with demethylating agents such as decitabine may affect chemotherapy outcome by modulation of apoptosis- relevant genes by methylation. More importantly, this modulation seems to be dependent on
{"title":"Changes in Gene Methylation Following Chemotherapy in Breast Cancer Cell Lines","authors":"F. Ari, R. Napieralski, E. Ulukaya","doi":"10.5505/TJB.2013.46320","DOIUrl":"https://doi.org/10.5505/TJB.2013.46320","url":null,"abstract":"Objective: Epigenetic modulation of gene expression by DNA promoter methylation may contribute to acquired resistance to chemotherapy in cancer cells. Decitabine (5-aza-2'- deoxycytidine), a demethylating agent, may act synergistically with standard chemotherapy regimens to activate epigenetically silenced genes. In the present in vitro study, it was investigated the effect of gene methylation level after treatment with decitabine and combination of decitabine with anthracycline-based therapeutics (5-fluorouracil plus epirubicine plus cyclophosphamide; FEC) on breast cancer cells (MCF-7 and MDA-MB-231). Methods: The effect of decitabine and its combination with FEC on different genes methylation level has been tested in MDA-MB-231 and MCF-7 human breast cancer cell lines. The effect of decitabine on the cell viability was assayed by MTT assay. Methylight real-time PCR and methylation specific PCR were carried out to determine the methylation status of certain genes: DAPK, TMS1, MGMT and the global methylation marker LINE-1. Results: The LINE-1 methylation status significantly decreased in both cell lines after treatment with the combination of decitabine with FEC. In MDA-MB-231 cells, methylation of the TMS1 and the MGMT gene promoter was significantly reduced by FEC plus decitabine while no effect was observed in MCF-7 cells. Conclusion: Anthracycline-based therapy regimens in combination with demethylating agents such as decitabine may affect chemotherapy outcome by modulation of apoptosis- relevant genes by methylation. More importantly, this modulation seems to be dependent on","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73990593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Ozakpinar, A. Maurer, C. Adiguzel, O. T. Cilingir, M. Demi̇r, F. Uras
Objective: Thrombocytopenia remains a serious problem in patients treated with highdose chemotherapy and bone marrow transplantation. In recent years, infusion of ex vivo expanded megakaryocytes (Mk) progenitors into patients has been proposed as a strategy for shortening the time of platelet engraftment. The development of in vitro culture methods to obtain sufficient numbers of Mks from haematopoietic stem cells (HSC) is an important target in basic and clinical research projects. The aim of this study was to develop a two-step ex vivo expansion culture system of Mk progenitors from peripheral blood stem cells (PBSC). Methods: PBSC were harvested from three healthy adult donors. CD34+ cells were isolated and cultured in serum free media supplemented with thrombopoietin (TPO) (50 ng/ml), Interleukin 3 (IL-3) (20 ng/ml) and Interleukin 6 (IL-6) (20 ng/ml) for 12 days followed by an incubation with IL-6 (20 ng/ml) and TPO (50 ng/ml) for another 9 days. The differentiation of Mks was monitored by flow cytometry (% of CD34+/41+ cells). The morphology of the cells was studied by light, electron and fluorescence microscopy. Results: Morphological analysis of cells generated after 7 days of culture showed typical aspects of developing Mks. The percentage of CD41+ cells was higher than 70 on day 21. Conclusion: The results obtained in this study demonstrated that this two-step culture system is an effective method to obtain high rates of megakaryocytes. It is obvious that this promising method needs further development for clinical applications.
{"title":"Differentiation of hematopoietic stem cells isolated from peripheral blood to megakaryocyte","authors":"O. Ozakpinar, A. Maurer, C. Adiguzel, O. T. Cilingir, M. Demi̇r, F. Uras","doi":"10.5505/TJB.2013.52714","DOIUrl":"https://doi.org/10.5505/TJB.2013.52714","url":null,"abstract":"Objective: Thrombocytopenia remains a serious problem in patients treated with highdose chemotherapy and bone marrow transplantation. In recent years, infusion of ex vivo expanded megakaryocytes (Mk) progenitors into patients has been proposed as a strategy for shortening the time of platelet engraftment. The development of in vitro culture methods to obtain sufficient numbers of Mks from haematopoietic stem cells (HSC) is an important target in basic and clinical research projects. The aim of this study was to develop a two-step ex vivo expansion culture system of Mk progenitors from peripheral blood stem cells (PBSC). Methods: PBSC were harvested from three healthy adult donors. CD34+ cells were isolated and cultured in serum free media supplemented with thrombopoietin (TPO) (50 ng/ml), Interleukin 3 (IL-3) (20 ng/ml) and Interleukin 6 (IL-6) (20 ng/ml) for 12 days followed by an incubation with IL-6 (20 ng/ml) and TPO (50 ng/ml) for another 9 days. The differentiation of Mks was monitored by flow cytometry (% of CD34+/41+ cells). The morphology of the cells was studied by light, electron and fluorescence microscopy. Results: Morphological analysis of cells generated after 7 days of culture showed typical aspects of developing Mks. The percentage of CD41+ cells was higher than 70 on day 21. Conclusion: The results obtained in this study demonstrated that this two-step culture system is an effective method to obtain high rates of megakaryocytes. It is obvious that this promising method needs further development for clinical applications.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5505/TJB.2013.52714","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72508886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Ç. Madenci, N. Yucel, Z. Arıkan, M. Sargin, Derya Akbaba, O. Kaya, A. Kaptanagasi
Objective: The relationship of asymmetrical dimethylarginine (ADMA) and glycemic control in diabetes is not yet fully enlightened. We aim to investigate the association of ADMA and hemoglobin A1c (HbA1c) in normal glomerular filtration rate (GFR) diabetic patients. Methods: This cross sectional study included 88 diabetic patients whose GFRs were in reference range. 2 different HbA1c values; current (cHbA1c) and mean of 4 successive measurements with 3 months intervals (mHbA1c) were used. The association of ADMA with HbA1c levels and other clinical characteristics of patients were evaluated. Results: We found significant inverse correlations between ADMA and both current (r =-0,354, p=0,001) and mean HbA1c(r=-0,377, p=0,000) levels. In multiple lineer regression analyses mHbA1c, glucose and duration of diabetes ( R²=0,343, p=0,000) or cHbA1c, glucose and duration of diabetes (R²=0,318 p<0,001)were predictive variables for ADMA
{"title":"The Relationship Between Glycemic Control And Asymmetrical Dimethylarginine Levels","authors":"O. Ç. Madenci, N. Yucel, Z. Arıkan, M. Sargin, Derya Akbaba, O. Kaya, A. Kaptanagasi","doi":"10.5505/TJB.2013.43153","DOIUrl":"https://doi.org/10.5505/TJB.2013.43153","url":null,"abstract":"Objective: The relationship of asymmetrical dimethylarginine (ADMA) and glycemic control in diabetes is not yet fully enlightened. We aim to investigate the association of ADMA and hemoglobin A1c (HbA1c) in normal glomerular filtration rate (GFR) diabetic patients. Methods: This cross sectional study included 88 diabetic patients whose GFRs were in reference range. 2 different HbA1c values; current (cHbA1c) and mean of 4 successive measurements with 3 months intervals (mHbA1c) were used. The association of ADMA with HbA1c levels and other clinical characteristics of patients were evaluated. Results: We found significant inverse correlations between ADMA and both current (r =-0,354, p=0,001) and mean HbA1c(r=-0,377, p=0,000) levels. In multiple lineer regression analyses mHbA1c, glucose and duration of diabetes ( R²=0,343, p=0,000) or cHbA1c, glucose and duration of diabetes (R²=0,318 p<0,001)were predictive variables for ADMA","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77443516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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