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Contribution of hepatitis B virus X protein-induced aberrant microRNA expression to hepatocellular carcinoma pathogenesis 乙型肝炎病毒X蛋白诱导的微小RNA表达异常对肝细胞癌发病机制的影响
IF 2.2 4区 生物学 Q1 Agricultural and Biological Sciences Pub Date : 2019-04-01 DOI: 10.3906/biy-1807-196
Zhiyuan Wei, Xiaohe Shen, B. Ni, G. Luo, Yi Tian, Yi Sun
The hepatitis B virus-encoded X (HBX) protein plays important roles in Hepatocellular carcinoma (HCC). Previous studies have demonstrated that HBX can induce alterations in the expression of numerous microRNAs (miRNAs) involved in the carcinogenesis of various tumors. However, the global profile of liver miRNA changes induced by HBX has not been characterized. In this study, we conducted a miRNA microarray analysis to investigate the influence of HBX on the expression of total miRNAs in liver in relation to HCC. Comparative analysis of the data from human normal liver cells (L02) and human HCC cells (HepG2), with or without HBX, identified 19 differentially expressed miRNAs, including 5 with known association to HBX. Target gene prediction for the aberrantly expressed miRNAs identified a total of 304 potential target genes, involved in sundry pathways. Finally, pathway analysis of the HBXinduced miRNAs pathway showed that 5 of the total miRNAs formed an internetwork, suggesting that HBX might exert its pathological effects on hepatic cells through functional synergy with miRNAs that regulated common pathways in liver cells. Therefore, this work provides new insights into the mechanisms of HCC as well as potential diagnostic markers or therapeutic targets for use in clinical management of HCC.
乙型肝炎病毒编码的X (HBX)蛋白在肝细胞癌(HCC)中起重要作用。先前的研究表明,HBX可以诱导多种参与各种肿瘤癌变的microrna (mirna)的表达改变。然而,HBX诱导的肝脏miRNA变化的全局概况尚未表征。在本研究中,我们通过miRNA微阵列分析来研究HBX对肝细胞癌相关肝脏中总miRNA表达的影响。对人正常肝细胞(L02)和人HCC细胞(HepG2)的数据进行比较分析,发现了19个差异表达的mirna,其中5个已知与HBX相关。靶基因预测异常表达的mirna共鉴定出304个潜在靶基因,涉及各种途径。最后,对hbxinducducedmirnas通路进行通路分析,发现其中5个miRNAs形成了一个网络,提示HBX可能通过与调节肝细胞常见通路的miRNAs的功能协同作用,对肝细胞产生病理作用。因此,这项工作为HCC的机制以及HCC临床管理中潜在的诊断标志物或治疗靶点提供了新的见解。
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引用次数: 3
Effects of various preculture, pretreatment, and recovery conditions on the morphogenetic response of cryopreserved Lady Orange chrysanthemum shoot tips 不同预培养、预处理和恢复条件对冬青菊花茎尖形态发生反应的影响
IF 2.2 4区 生物学 Q1 Agricultural and Biological Sciences Pub Date : 2018-02-15 DOI: 10.3906/BIY-1711-47
D. Kulus
The aim of this research was to optimize the encapsulation-dehydration cryopreservation protocol of Lady Orange chrysanthemum explants on the preculture, pretreatment, and post-rewarming recovery steps. Shoot tips were precultured on MS medium with various abscisic acid concentrations (0-30 µM). Next, the encapsulated explants were osmotically dehydrated for 3 or 5 days and desiccated for 3-4 h and, after rewarming, they were subcultured on recovery media of various compositions (control and cytokinin/auxin-supplemented). A high explant survival rate, even up to 100%, was observed. The value of this parameter, however, changed depending on the post-rewarming culture duration. Moreover, not all the viable explants were capable of forming shoots. A lower ABA concentration (15 µM) during preculture and the presence of 4.65 µM kinetin in the post-rewarming recovery medium enhanced cryopreservation efficiency with a high survival rate and typical microshoot formation. A higher ABA concentration and the presence of 6-benzylaminopurine in the recovery medium resulted in shoot multiplication, abundant callus formation, and root formation inhibition.
本研究的目的是在预培养、预处理和复温后恢复步骤上优化Lady Orange菊花外植体的包埋脱水冷冻保存方案。茎尖在具有不同脱落酸浓度(0-30µM)的MS培养基上预培养。接下来,将包埋的外植体渗透脱水3或5天并干燥3-4小时,并且在复温后,将它们在各种组成的回收培养基(补充对照和细胞分裂素/生长素)上传代培养。观察到高的外植体存活率,甚至高达100%。然而,此参数的值会根据复温后培养的持续时间而变化。此外,并非所有有活力的外植体都能形成芽。预培养过程中较低的ABA浓度(15µM)和复温后恢复培养基中4.65µM激动素的存在提高了冷冻保存效率,具有高存活率和典型的微芽形成。较高的ABA浓度和回收培养基中6-苄基氨基嘌呤的存在导致芽增殖、大量愈伤组织形成和根形成抑制。
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引用次数: 10
Comparative proteomic analysis of Bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis 苏云金芽孢杆菌野生型和两个多磷酸盐稳态紊乱突变株的蛋白质组学比较分析
IF 2.2 4区 生物学 Q1 Agricultural and Biological Sciences Pub Date : 2018-02-15 DOI: 10.3906/BIY-1711-9
Filiz Yesilirmak, Tuğrul Doruk, S. Yilmaz, Sedef Tunca Gedik
The Scientific and Technological Research Council of Turkey [TBAG-107T812, TBAG-111T047, KBAG-113Z898]
土耳其科学技术研究委员会[TBAG-107T812,TBAG-111T047,KBAG-113Z898]
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引用次数: 2
In vitro transcription and validation of human pancreatic transcription factors' mRNAs 人胰腺转录因子mrna的体外转录及验证
IF 2.2 4区 生物学 Q1 Agricultural and Biological Sciences Pub Date : 2017-11-10 DOI: 10.3906/BIY-1610-29
Ersin Akinci, M. Yildiz, P. Unal, Gamze Badakul
* Correspondence: ersinakinci@akdeniz.edu.tr
*通信:ersinakinci@akdeniz.edu.tr
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引用次数: 0
N-Acetylglucoseamine modified alginate sponges as scaffolds for skin tissue engineering n -乙酰氨基葡萄糖修饰海藻酸盐海绵作为皮肤组织工程支架
IF 2.2 4区 生物学 Q1 Agricultural and Biological Sciences Pub Date : 2017-10-01 DOI: 10.3906/BIY-1704-31
M. Demirbilek, N. Türkoğlu, S. Aktürk
In the treatment of dermal wounds, wound-dressing materials prepared from natural mucopolysaccharides are widely used because of their advantages such as nonirritation, nontoxicity, and ease in topical application. In the present study, alginate hydrogels modified with N-acetyl glucose amine (NAG) were prepared as wound-dressing material. Physical, chemical, thermal, and mechanical properties of the hydrogels were studied. Cytotoxicity of the hydrogels on endothelial (HUVEC) and keratinocyte (HaCaT) cells were examined. Anti- and proinflammatory cytokine levels of human monocyte-macrophage cells (THP-1) stimulated with hydrogels were determined. According to the results, increasing the NAG concentration led to an increase in the swelling and nitrogen ratios in the hydrogels. Additionally, increasing the NAG concentration decreased elastic modulus and degradation time. Hydrogels were not cytotoxic on HaCaT and HUVEC cells. It stimulated IL-10 and TNF-alpha levels at a small rate.
在真皮伤口的治疗中,由天然粘多糖制备的伤口敷料因其无刺激性、无毒性和易于局部应用等优点而得到广泛应用。本研究制备了N-乙酰葡糖胺(NAG)修饰的海藻酸盐水凝胶作为伤口敷料材料。研究了水凝胶的物理、化学、热学和力学性能。研究了水凝胶对内皮细胞(HUVEC)和角质形成细胞(HaCaT)的细胞毒性。测定了水凝胶刺激的人单核细胞-巨噬细胞(THP-1)的抗炎和促炎细胞因子水平。根据结果,增加NAG浓度导致水凝胶中溶胀率和氮比增加。此外,增加NAG浓度降低了弹性模量和降解时间。水凝胶对HaCaT和HUVEC细胞没有细胞毒性。它以较小的速率刺激IL-10和TNF-α水平。
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引用次数: 6
Proteomics analysis of mitochondrial dysfunction triggered by complex specific electron transport chain inhibitors reveals common pathways involving protein misfolding in an SH-SY5Y in vitro cell model 复杂特异性电子传递链抑制剂引发线粒体功能障碍的蛋白质组学分析揭示了SH-SY5Y体外细胞模型中涉及蛋白质错误折叠的常见途径
IF 2.2 4区 生物学 Q1 Agricultural and Biological Sciences Pub Date : 2017-10-01 DOI: 10.3906/BIY-1702-44
B. Sahin, A. Baykal
Mitochondrial dysfunction has been previously identified in neurodegenerative diseases such as Alzheimer disease, Huntington disease, and Parkinson disease. Chemical inhibition of the mitochondrial electron transport chain (ETC) was shown to trigger symptoms in animal models similar to those observed in human neurodegenerative diseases. In order to understand the effect of mitochondrial dysfunction on the proteome level, LC-MSE-based bottom-up, label-free differential proteomics expression analysis was used to monitor protein level changes in SH-SY5Y neuroblastoma cells induced by ETC-specific inhibitors (MPTP, 3-NP, sodium azide, antimycin A, and oligomycin). A total of 379 proteins were identified across the sample set and 75 of them were found to be differentially expressed (>30% fold change). Complex-specific inhibition of the five ETS complexes were expected to result in the aberrant regulation of different molecular pathways, but the bioinformatics analysis of the LC-MSMS data showed that the differentially expressed proteins were mostly involved in similar metabolic processes. The findings suggest that the complex-specific alterations may not be directly linked to neurodegenerative pathways, but could be considered contributors. Moreover, the proteins that showed the highest protein expression difference (>60% fold change) are involved in pathways regarding protein-folding and response to unfolded proteins. The results indicate that protein misfolding pathways might have a central role in the genesis and progression of neurodegenerative diseases and that label-free LC-MSMS proteomics analysis is an invaluable approach for studying of molecular pathways in neurodegeneration.
线粒体功能障碍以前在神经退行性疾病如阿尔茨海默病、亨廷顿病和帕金森病中被发现。在动物模型中,线粒体电子传递链(ETC)的化学抑制被证明会引发类似于在人类神经退行性疾病中观察到的症状。为了了解线粒体功能障碍对蛋白质组水平的影响,采用lc - mse自底向上、无标记的差异蛋白质组学表达分析,监测etc特异性抑制剂(MPTP、3-NP、叠氮化钠、抗霉素A和寡霉素)诱导SH-SY5Y神经母细胞瘤细胞的蛋白质水平变化。在整个样本集中共鉴定出379个蛋白,其中75个蛋白被发现是差异表达(bbb30倍变化)。五种ETS复合物的特异性抑制被认为会导致不同分子通路的异常调节,但LC-MSMS数据的生物信息学分析显示,差异表达的蛋白大多参与类似的代谢过程。研究结果表明,复杂特异性的改变可能与神经退行性通路没有直接联系,但可以被认为是贡献者。此外,显示最高蛋白表达差异(bbb60 %折叠变化)的蛋白参与蛋白质折叠和对未折叠蛋白的反应途径。结果表明,蛋白质错误折叠途径可能在神经退行性疾病的发生和发展中起核心作用,无标记LC-MSMS蛋白质组学分析是研究神经退行性疾病分子途径的宝贵方法。
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引用次数: 1
Extracellular presence/release of galectins from HTR-8/SVneo extravillous trophoblast cells* HTR-8/SVneo滋养细胞胞外存在/释放凝集素*
IF 2.2 4区 生物学 Q1 Agricultural and Biological Sciences Pub Date : 2017-10-01 DOI: 10.3906/BIY-1704-11
D. Ćujić, M. Kosanović, M. J. Krivokuća, L. Vićovac, M. Janković
Galectins (gals) are s-galactoside binding lectins, involved in many processes at the fetomaternal interface where they can exert their roles both in and out of cells. The aim of this work was to explore the extracellular presence/release of HTR-8/SVneo extravillous trophoblast cell line galectins. To that end, conditioned medium (CM) from HTR-8/SVneo cell culture was fractionated into a high molecular mass fraction (HMF) and low molecular mass fraction (LMF) using 100 kDa cut-off concentrators. In addition, extracellular vesicles (EVs) were isolated from CM by ultracentrifugation. Size and shape of the EVs were analyzed by transmission electron microscopy and their galectin mRNA content was determined by real-time PCR. The presence of galectins in fractions of HTR-8/SVneo CM and EVs was detected by western blot. All three galectins expressed by HTR-8/SVneo cells (gal-1, gal-3, and gal-8) were detected in the CM, HMF, and EVs, while only gal-1 was found in the LMF. In addition, EVs contained all three galectin mRNAs. These results reveal that free, complexed, and EV-associated forms of galectins were released from extravillous trophoblast cells, suggesting their potential to exert extracellular functions both in their immediate vicinity at the fetomaternal interface and distant locations.
半乳糖凝集素(gals)是s-半乳糖苷结合凝集素,参与胎儿与母体界面的许多过程,它们可以在细胞内外发挥作用。本工作的目的是探索HTR-8/SVneo绒毛外滋养层细胞系半乳糖凝集素在细胞外的存在/释放。为此,使用100kDa截止浓缩器将来自HTR-8/SVneo细胞培养物的条件培养基(CM)分级为高分子量部分(HMF)和低分子量部分(LMF)。此外,通过超速离心从CM中分离出细胞外小泡(EVs)。通过透射电子显微镜分析EVs的大小和形状,并通过实时PCR测定其半乳糖凝集素mRNA含量。通过蛋白质印迹检测HTR-8/SVneo-CM和EVs的级分中半乳糖凝集素的存在。HTR-8/SVneo细胞表达的所有三种半乳糖凝集素(gal-1、gal-3和gal-8)均在CM、HMF和EVs中检测到,而在LMF中仅发现gal-1。此外,EVs含有所有三种半乳糖凝集素mRNA。这些结果表明,绒毛外滋养层细胞释放出游离的、复合的和EV相关形式的半乳糖凝集素,这表明它们有可能在母胎界面附近和远处发挥细胞外功能。
{"title":"Extracellular presence/release of galectins from HTR-8/SVneo extravillous trophoblast cells*","authors":"D. Ćujić, M. Kosanović, M. J. Krivokuća, L. Vićovac, M. Janković","doi":"10.3906/BIY-1704-11","DOIUrl":"https://doi.org/10.3906/BIY-1704-11","url":null,"abstract":"Galectins (gals) are s-galactoside binding lectins, involved in many processes at the fetomaternal interface where they can exert their roles both in and out of cells. The aim of this work was to explore the extracellular presence/release of HTR-8/SVneo extravillous trophoblast cell line galectins. To that end, conditioned medium (CM) from HTR-8/SVneo cell culture was fractionated into a high molecular mass fraction (HMF) and low molecular mass fraction (LMF) using 100 kDa cut-off concentrators. In addition, extracellular vesicles (EVs) were isolated from CM by ultracentrifugation. Size and shape of the EVs were analyzed by transmission electron microscopy and their galectin mRNA content was determined by real-time PCR. The presence of galectins in fractions of HTR-8/SVneo CM and EVs was detected by western blot. All three galectins expressed by HTR-8/SVneo cells (gal-1, gal-3, and gal-8) were detected in the CM, HMF, and EVs, while only gal-1 was found in the LMF. In addition, EVs contained all three galectin mRNAs. These results reveal that free, complexed, and EV-associated forms of galectins were released from extravillous trophoblast cells, suggesting their potential to exert extracellular functions both in their immediate vicinity at the fetomaternal interface and distant locations.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2017-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1704-11","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48104352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Silver nanoparticles induced genotoxicity and oxidative stress in tomato plants 银纳米粒子诱导番茄植株的遗传毒性和氧化应激
IF 2.2 4区 生物学 Q1 Agricultural and Biological Sciences Pub Date : 2017-10-01 DOI: 10.3906/BIY-1608-36
F. Çekiç, Sefa Ekinci, Muslum S. Inal, D. Ozakca
Among nanoparticles, silver nanoparticles (AgNPs) are intensively used in many materials owing to their antibacterial effects. In the present study different concentrations of AgNPs in Hoagland solution were applied to tomato seedlings. Total chlorophyll content, relative water content (RWC), antioxidant enzyme activities, and malondialdehyde content (MDA) as well as the genomic template stability (GTS) were analyzed. The intersimple sequence repeat polymerase chain reaction assay (ISSR-PCR) was used to determine the genotoxic effects of AgNPs on DNA. RWC did not change under AgNPs treatments; however, total chlorophyll content was significantly reduced by AgNPs applications. ISSR profiles demonstrated a consistent increase in polymorphic bands by the increase in the concentration of AgNPs. GTS value was also reduced depending on the concentration of AgNPs. SOD and APX activities were increased under low AgNPs treatments; however, these activities were decreased under high concentrations of AgNPs treatments. Tomato plants could be sensitive to AgNPs within the increase in MDA content in all of the AgNPs treatments. AgNPs nanotoxicity could be quite dose-dependent. AgNPs could also have negative effects on tomato plants by enhancing DNA damage and lipid peroxidation.
在纳米颗粒中,银纳米颗粒由于其抗菌作用而被广泛应用于许多材料中。在本研究中,将Hoagland溶液中不同浓度的AgNPs应用于番茄幼苗。分析了总叶绿素含量、相对含水量(RWC)、抗氧化酶活性、丙二醛含量(MDA)以及基因组模板稳定性(GTS)。单序列间重复聚合酶链式反应(ISSR-PCR)用于测定AgNPs对DNA的遗传毒性作用。在AgNPs处理下RWC没有变化;然而,施用AgNPs显著降低了总叶绿素含量。ISSR图谱显示,随着AgNPs浓度的增加,多态性条带持续增加。GTS值也根据AgNPs的浓度而降低。在低AgNPs处理下,SOD和APX活性增加;然而,在高浓度的AgNPs处理下,这些活性降低。在所有AgNPs处理中,番茄植株对MDA含量的增加可能对AgNPs敏感。AgNPs的纳米毒性可能具有相当的剂量依赖性。AgNPs也可能通过增强DNA损伤和脂质过氧化对番茄植株产生负面影响。
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引用次数: 34
Interaction of adipose-derived mesenchymal stem cells with MCF-7 cells in vitro:a study emphasizing signaling molecule expression and transcriptional changes 脂肪源性间充质干细胞与MCF-7细胞的相互作用:一项强调信号分子表达和转录变化的研究
IF 2.2 4区 生物学 Q1 Agricultural and Biological Sciences Pub Date : 2017-10-01 DOI: 10.3906/BIY-1701-23
Qiong Wu, Chang-xin Jin, Hui Chen, Xueyang Li, Yue Li
Adipose-derived stem cells (ADSCs) promote metastasis of breast cancer cells that can differentiate into carcinoma-associated cells in tumor microenvironments. However, the precise mechanism is poorly understood. This study shows that interaction of ADSCs with breast cancer MCF-7 cells changes the level of metastasis-related functional proteins in MCF-7 cells, as well as that of oncogenes in ADSCs. ADSCs were isolated from adipose tissues of patients. The interaction of ADSCs with MCF-7 cells was performed by coculturing ADSCs (or MCF-7 cells) with exosomes derived from MCF-7 cells (or ADSCs). Exosomes were labeled by DiI. In cocultures, migration-related regulators in MCF-7 cells were significantly enhanced in both protein (Smad, Slug, Snail1/2, Twist1, N-cadherin, vimentin) and mRNA (SMAD, SLUG, SNAIL1/2, TWIST1, N-cadherin, and vimentin) levels. The expression levels of oncogenes (RAS and HER-2) and an antioncogene gene (P53) in ADSCs were upregulated and downregulated, respectively. Interestingly, variation tendencies of the molecules were more conspicuous in inflammatory circumstances than without. In conclusion, interaction of MCF-7 cells with ADSCs increases the levels of a series of metastasis-related functional proteins in MCF-7 cells and enhances the expression of oncogenes in ADSCs.
脂肪源性干细胞(ADSCs)促进乳腺癌细胞的转移,并能在肿瘤微环境中分化为癌相关细胞。然而,人们对其确切机制知之甚少。本研究表明,ADSCs与乳腺癌MCF-7细胞的相互作用改变了MCF-7细胞中转移相关功能蛋白的水平,也改变了ADSCs中癌基因的水平。从患者脂肪组织中分离ADSCs。通过将ADSCs(或MCF-7细胞)与MCF-7细胞(或ADSCs)衍生的外泌体共培养,ADSCs与MCF-7细胞相互作用。外泌体用DiI标记。在共培养中,MCF-7细胞中迁移相关调节因子的蛋白(Smad、Slug、Snail1/2、Twist1、N-cadherin、vimentin)和mRNA (Smad、Slug、Snail1/2、Twist1、N-cadherin和vimentin)水平均显著增强。癌基因(RAS和HER-2)和抗癌基因(P53)在ADSCs中的表达水平分别上调和下调。有趣的是,分子的变异趋势在炎症环境中比在没有炎症的情况下更为明显。综上所述,MCF-7细胞与ADSCs的相互作用增加了MCF-7细胞中一系列转移相关功能蛋白的水平,并增强了ADSCs中癌基因的表达。
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引用次数: 1
Effect of nutrient media and initial biomass on growth rate and nutrient uptake of Sargassum spinuligerum (Sargassaceae, Phaeophyta) 营养介质和初始生物量对刺马尾藻生长速率和养分吸收的影响
IF 2.2 4区 生物学 Q1 Agricultural and Biological Sciences Pub Date : 2017-10-01 DOI: 10.3906/BIY-1703-49
Tin C. Hoang, R. Fotedar, M. O’Leary
The Sargassum species are prospective candidates for marine culture, but there are a limited number of reports on their nutrient requirements and optimum initial stocking biomass, and nothing is published for Sargassum spinuligerum. This study investigated the effects of three commercially available fertilizers (Hortico, Seasol, and Aquasol) and four initial stocking biomass levels of S. spinuligerum on the growth rate and nutrient uptake capacities for 7 weeks. The results showed that S. spinuligerum could be grown under outdoor conditions with the optimum initial stocking biomass of 15.35 g per 113 L. The different commercial fertilizers significantly influenced the specific growth rate and nutrient uptake rate of S. spinuligerum. Aquasol resulted in a higher specific growth rate than the other commercial fertilizers, with the relative growth rate fluctuating between 0.42 and 1.70 (% per day). Aquasol is recommended as a nutrient supplement to enhance the specific growth rate of S. spinuligerum.
马尾藻是海洋养殖的潜在候选者,但关于其营养需求和最佳初始放养生物量的报道数量有限,关于刺马尾藻的报道也没有发表。本研究调查了三种市售肥料(Hortico、Seasol和Aquasol)和刺五加四种初始放养生物量水平对生长速率和养分吸收能力的影响,为期7周。结果表明,在室外条件下可以生长刺五加,最适初始放养生物量为15.35g/113L。不同的商品肥料对刺五加的比生长速率和养分吸收速率有显著影响。Aquasol的比生长率高于其他商业肥料,相对生长率在0.42至1.70之间波动(每天%)。Aquasol被推荐作为一种营养补充剂,以提高S.spinuligerum的特定生长速率。
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引用次数: 0
期刊
Turkish Journal of Biology
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