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Silver nanoparticles induced genotoxicity and oxidative stress in tomato plants 银纳米粒子诱导番茄植株的遗传毒性和氧化应激
IF 2.2 4区 生物学 Q3 BIOLOGY Pub Date : 2017-10-01 DOI: 10.3906/BIY-1608-36
F. Çekiç, Sefa Ekinci, Muslum S. Inal, D. Ozakca
Among nanoparticles, silver nanoparticles (AgNPs) are intensively used in many materials owing to their antibacterial effects. In the present study different concentrations of AgNPs in Hoagland solution were applied to tomato seedlings. Total chlorophyll content, relative water content (RWC), antioxidant enzyme activities, and malondialdehyde content (MDA) as well as the genomic template stability (GTS) were analyzed. The intersimple sequence repeat polymerase chain reaction assay (ISSR-PCR) was used to determine the genotoxic effects of AgNPs on DNA. RWC did not change under AgNPs treatments; however, total chlorophyll content was significantly reduced by AgNPs applications. ISSR profiles demonstrated a consistent increase in polymorphic bands by the increase in the concentration of AgNPs. GTS value was also reduced depending on the concentration of AgNPs. SOD and APX activities were increased under low AgNPs treatments; however, these activities were decreased under high concentrations of AgNPs treatments. Tomato plants could be sensitive to AgNPs within the increase in MDA content in all of the AgNPs treatments. AgNPs nanotoxicity could be quite dose-dependent. AgNPs could also have negative effects on tomato plants by enhancing DNA damage and lipid peroxidation.
在纳米颗粒中,银纳米颗粒由于其抗菌作用而被广泛应用于许多材料中。在本研究中,将Hoagland溶液中不同浓度的AgNPs应用于番茄幼苗。分析了总叶绿素含量、相对含水量(RWC)、抗氧化酶活性、丙二醛含量(MDA)以及基因组模板稳定性(GTS)。单序列间重复聚合酶链式反应(ISSR-PCR)用于测定AgNPs对DNA的遗传毒性作用。在AgNPs处理下RWC没有变化;然而,施用AgNPs显著降低了总叶绿素含量。ISSR图谱显示,随着AgNPs浓度的增加,多态性条带持续增加。GTS值也根据AgNPs的浓度而降低。在低AgNPs处理下,SOD和APX活性增加;然而,在高浓度的AgNPs处理下,这些活性降低。在所有AgNPs处理中,番茄植株对MDA含量的增加可能对AgNPs敏感。AgNPs的纳米毒性可能具有相当的剂量依赖性。AgNPs也可能通过增强DNA损伤和脂质过氧化对番茄植株产生负面影响。
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引用次数: 34
Interaction of adipose-derived mesenchymal stem cells with MCF-7 cells in vitro:a study emphasizing signaling molecule expression and transcriptional changes 脂肪源性间充质干细胞与MCF-7细胞的相互作用:一项强调信号分子表达和转录变化的研究
IF 2.2 4区 生物学 Q3 BIOLOGY Pub Date : 2017-10-01 DOI: 10.3906/BIY-1701-23
Qiong Wu, Chang-xin Jin, Hui Chen, Xueyang Li, Yue Li
Adipose-derived stem cells (ADSCs) promote metastasis of breast cancer cells that can differentiate into carcinoma-associated cells in tumor microenvironments. However, the precise mechanism is poorly understood. This study shows that interaction of ADSCs with breast cancer MCF-7 cells changes the level of metastasis-related functional proteins in MCF-7 cells, as well as that of oncogenes in ADSCs. ADSCs were isolated from adipose tissues of patients. The interaction of ADSCs with MCF-7 cells was performed by coculturing ADSCs (or MCF-7 cells) with exosomes derived from MCF-7 cells (or ADSCs). Exosomes were labeled by DiI. In cocultures, migration-related regulators in MCF-7 cells were significantly enhanced in both protein (Smad, Slug, Snail1/2, Twist1, N-cadherin, vimentin) and mRNA (SMAD, SLUG, SNAIL1/2, TWIST1, N-cadherin, and vimentin) levels. The expression levels of oncogenes (RAS and HER-2) and an antioncogene gene (P53) in ADSCs were upregulated and downregulated, respectively. Interestingly, variation tendencies of the molecules were more conspicuous in inflammatory circumstances than without. In conclusion, interaction of MCF-7 cells with ADSCs increases the levels of a series of metastasis-related functional proteins in MCF-7 cells and enhances the expression of oncogenes in ADSCs.
脂肪源性干细胞(ADSCs)促进乳腺癌细胞的转移,并能在肿瘤微环境中分化为癌相关细胞。然而,人们对其确切机制知之甚少。本研究表明,ADSCs与乳腺癌MCF-7细胞的相互作用改变了MCF-7细胞中转移相关功能蛋白的水平,也改变了ADSCs中癌基因的水平。从患者脂肪组织中分离ADSCs。通过将ADSCs(或MCF-7细胞)与MCF-7细胞(或ADSCs)衍生的外泌体共培养,ADSCs与MCF-7细胞相互作用。外泌体用DiI标记。在共培养中,MCF-7细胞中迁移相关调节因子的蛋白(Smad、Slug、Snail1/2、Twist1、N-cadherin、vimentin)和mRNA (Smad、Slug、Snail1/2、Twist1、N-cadherin和vimentin)水平均显著增强。癌基因(RAS和HER-2)和抗癌基因(P53)在ADSCs中的表达水平分别上调和下调。有趣的是,分子的变异趋势在炎症环境中比在没有炎症的情况下更为明显。综上所述,MCF-7细胞与ADSCs的相互作用增加了MCF-7细胞中一系列转移相关功能蛋白的水平,并增强了ADSCs中癌基因的表达。
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引用次数: 1
Effect of nutrient media and initial biomass on growth rate and nutrient uptake of Sargassum spinuligerum (Sargassaceae, Phaeophyta) 营养介质和初始生物量对刺马尾藻生长速率和养分吸收的影响
IF 2.2 4区 生物学 Q3 BIOLOGY Pub Date : 2017-10-01 DOI: 10.3906/BIY-1703-49
Tin C. Hoang, R. Fotedar, M. O’Leary
The Sargassum species are prospective candidates for marine culture, but there are a limited number of reports on their nutrient requirements and optimum initial stocking biomass, and nothing is published for Sargassum spinuligerum. This study investigated the effects of three commercially available fertilizers (Hortico, Seasol, and Aquasol) and four initial stocking biomass levels of S. spinuligerum on the growth rate and nutrient uptake capacities for 7 weeks. The results showed that S. spinuligerum could be grown under outdoor conditions with the optimum initial stocking biomass of 15.35 g per 113 L. The different commercial fertilizers significantly influenced the specific growth rate and nutrient uptake rate of S. spinuligerum. Aquasol resulted in a higher specific growth rate than the other commercial fertilizers, with the relative growth rate fluctuating between 0.42 and 1.70 (% per day). Aquasol is recommended as a nutrient supplement to enhance the specific growth rate of S. spinuligerum.
马尾藻是海洋养殖的潜在候选者,但关于其营养需求和最佳初始放养生物量的报道数量有限,关于刺马尾藻的报道也没有发表。本研究调查了三种市售肥料(Hortico、Seasol和Aquasol)和刺五加四种初始放养生物量水平对生长速率和养分吸收能力的影响,为期7周。结果表明,在室外条件下可以生长刺五加,最适初始放养生物量为15.35g/113L。不同的商品肥料对刺五加的比生长速率和养分吸收速率有显著影响。Aquasol的比生长率高于其他商业肥料,相对生长率在0.42至1.70之间波动(每天%)。Aquasol被推荐作为一种营养补充剂,以提高S.spinuligerum的特定生长速率。
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引用次数: 0
Effects of celecoxib and L-NAME on apoptosis and cell cycle ofMCF-7 CD44+/CD24-/low subpopulation 塞来昔布和L-NAME对MCF-7 CD44+/CD24-/低亚群细胞凋亡和细胞周期的影响
IF 2.2 4区 生物学 Q3 BIOLOGY Pub Date : 2017-10-01 DOI: 10.3906/BIY-1703-101
M. Majdzadeh, Shima Aliebrahimi, Melody Vatankhah, S. Ostad
Recent studies have reported that cancer stem cells (CSCs) play a pivotal role in treatment failure, causing cancer recurrence. Here, we investigated the effects of L-NAME (an iNOS inhibitor) and celecoxib (a selective COX-2 inhibitor) on CSC-like cells (CSC-LCs) and their parental cells. Breast CSC-LCs derived from the MCF-7 cell line were sorted and characterized with the CD44+/CD24-/low phenotype. After isolation, the percentage of the subpopulation expressing CD44+/CD24-/low biomarkers increased considerably from 0.96% to 28.6%. Use of L-NAME and celecoxib showed antiproliferative activity towards both MCF-7 and CSC-LCs. Although celecoxib enhanced apoptotic cell death, the CSC-LC population was more resistant than parental cells. Moreover, L-NAME was less effective at inducing apoptosis, suggesting an involvement of different mechanisms of cell death. L-NAME caused cell cycle arrest in the S-phase in CSC-LCs, while celecoxib induced G0/G1 arrest in CSC-LCs and their parental cells. Immunocytochemistry results demonstrated that L-NAME had a similar potency to attenuate iNOS expression in MCF-7 and CSC-LCs; however, celecoxib reduced COX-2 expression in MCF-7 cells. The results show the crucial role of NOS and COX-2 in the maintenance of CD44+/CD24-/low breast CSC-LCs and suggest that L-NAME and celecoxib could have clinical implication in combination therapy.
最近的研究表明,癌症干细胞(CSCs)在治疗失败中起着关键作用,导致癌症复发。在此,我们研究了L-NAME(一种iNOS抑制剂)和塞来昔布(一种选择性COX-2抑制剂)对CSC样细胞(CSC-LCs)及其亲代细胞的影响。对来源于MCF-7细胞系的乳腺CSC LC进行分类,并以CD44+/CD24-/低表型进行表征。分离后,表达CD44+/CD24-/低生物标志物的亚群的百分比从0.96%显著增加到28.6%。L-NAME和塞来昔布的使用显示出对MCF-7和CSC-LCs的抗增殖活性。尽管塞来昔布增强了细胞凋亡,但CSC-LC群体比亲代细胞更具耐药性。此外,L-NAME在诱导细胞凋亡方面的效果较差,这表明其参与了不同的细胞死亡机制。L-NAME导致CSC-LCs的细胞周期停滞在S期,而塞来昔布诱导CSC-LCs及其亲代细胞的G0/G1停滞。免疫细胞化学结果表明,L-NAME在MCF-7和CSC-LCs中具有类似的减弱iNOS表达的效力;然而,塞来昔布降低了MCF-7细胞中COX-2的表达。结果显示NOS和COX-2在维持CD44+/CD24-/低乳腺CSC-LCs中的关键作用,并表明L-NAME和塞来昔布在联合治疗中可能具有临床意义。
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引用次数: 5
Effect of mutation in active site residue Trp209 to Val, Leu, Ile and Pro on the catalytic activity and affinity for some benzenesulfonamides of human carbonic anhydrase II 活性位点Trp209突变为Val、Leu、Ile和Pro对人碳酸酐酶II对某些苯磺酰胺的催化活性和亲和力的影响
IF 2.2 4区 生物学 Q3 BIOLOGY Pub Date : 2017-10-01 DOI: 10.3906/BIY-1705-37
Deryanur Kılıç, O. Erdoğan, Ö. Küfrevioğlu
Human carbonic anhydrase II (hCA II) enzyme was firstly expressed using a pET-SUMO expression vector in Escherichia coli and the recombinant enzyme was purified using nickel (Ni2+) affinity chromatography. The substitutions of Trp 209 with four amino acid (Val, Leu, Ile, and Pro) in the hydrophobic pocket of hCA II were conducted using site-directed mutagenesis. The p-nitrophenyl esterase activity of hCA II variants correlates with the hydrophobicity and size of residue, suggesting that the hydrophobic character of this residue is important for catalysis. The Trp 209 was forecast as an important residue and was exposed to computational mutagenesis. This forecast was confirmed experimentally by producing hCA II mutants and determining the resulting affinities towards some benzenesulfonamides. These mutations in the hydrophobic pocket of the enzyme active site decreased the protein expression of hCA II in E. coli, causing the formation of insoluble protein aggregates in many cases. Our findings demonstrated that the Trp 209 in hCA II plays an important role in the folding process and the valine residues are very compatible for the hydrophobic region in the active cavity of this isoenzyme. These mutant proteins will lead to a better understanding of structural functions and drug-based studies in the future.
首先用pET-SUMO表达载体在大肠杆菌中表达人碳酸酐酶II(hCAII),并用镍(Ni2+)亲和层析纯化重组酶。在hCA II的疏水口袋中用四个氨基酸(Val、Leu、Ile和Pro)取代Trp 209是使用定点突变进行的。hCA II变体的对硝基苯基酯酶活性与残基的疏水性和大小相关,表明该残基的亲水性对催化作用很重要。Trp 209被预测为一个重要的残基,并暴露于计算诱变。通过产生hCA II突变体并测定由此产生的对一些苯磺酰胺的亲和力,实验证实了这一预测。酶活性位点疏水口袋中的这些突变降低了大肠杆菌中hCA II的蛋白质表达,在许多情况下导致不溶性蛋白质聚集体的形成。我们的研究结果表明,hCA II中的Trp 209在折叠过程中起着重要作用,缬氨酸残基与该同工酶活性腔中的疏水区非常相容。这些突变蛋白将在未来更好地理解结构功能和基于药物的研究。
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引用次数: 1
Hybrid nanomaterial: biocolloidals 纳米材料混合物:生物胶体
IF 2.2 4区 生物学 Q3 BIOLOGY Pub Date : 2017-10-01 DOI: 10.3906/BIY-1705-31
Numan Gozubenli, Emir Yasun, N. Dilsiz
Nanomaterials-based diagnostics have tremendous advantages over other conventional diagnostic systems in terms of sensitivity, specificity, and portability owing to the unique intrinsic properties of nanomaterials. Thus, there is a substantial need to develop and employ a broad spectrum of nanomaterials for biological and diagnostic applications. In this review, we focus on nanomaterials-assisted disease diagnosis utilizing different techniques such as photoluminescence, colorimetry, fluorescence, electrochemistry, microarray methods, surface plasmon resonance, and surface-enhanced Raman spectroscopy. In these techniques, different nanomaterials, including but not limited to gold and silver materials, metal oxides, and semiconductors, were employed according to their all-purpose chemical and physical properties. As the new properties of nanomaterials are discovered, their contributions to nanobiotechnology applications are vastly increased. In this review, the features of the selected nanobiomaterials, biological tag-modified nanomaterials, and their outstanding applications for the detection of specific biotargets have been summarized according to the latest studies. Nanomaterials contribute to the fields of photo/chemotherapy and biomedical imaging in particular, since all the biological events happen within this size range and beneficiary roles of nanoparticles (NPs) are countless in biomedical applications due to their unique physical and chemical properties. However, due to the necessity of specificity and selectivity, there is a clear ambition to study bioconjugation of NPs to increase the efficiency of the targeted biological applications. Thus, the combination of the specificity and the intrinsic properties of biohybrid NPs facilitate diagnostic and therapeutic applications.
由于纳米材料独特的固有特性,基于纳米材料的诊断在灵敏度、特异性和便携性方面比其他传统诊断系统具有巨大优势。因此,迫切需要开发和使用广泛的纳米材料用于生物和诊断应用。在这篇综述中,我们重点关注纳米材料辅助疾病诊断,利用不同的技术,如光致发光、比色法、荧光、电化学、微阵列方法、表面等离子体共振和表面增强拉曼光谱。在这些技术中,根据其通用的化学和物理特性,使用了不同的纳米材料,包括但不限于金和银材料、金属氧化物和半导体。随着纳米材料的新特性被发现,它们对纳米生物技术应用的贡献大大增加。本文根据最新研究综述了所选纳米生物材料、生物标签修饰纳米材料的特点及其在检测特定生物靶标方面的突出应用。纳米材料特别有助于光/化学疗法和生物医学成像领域,因为所有的生物事件都发生在这个尺寸范围内,并且由于其独特的物理和化学性质,纳米颗粒在生物医学应用中的受益作用数不胜数。然而,由于特异性和选择性的必要性,研究NP的生物偶联以提高靶向生物应用的效率是一个明确的目标。因此,生物杂交NP的特异性和固有特性的结合促进了诊断和治疗应用。
{"title":"Hybrid nanomaterial: biocolloidals","authors":"Numan Gozubenli, Emir Yasun, N. Dilsiz","doi":"10.3906/BIY-1705-31","DOIUrl":"https://doi.org/10.3906/BIY-1705-31","url":null,"abstract":"Nanomaterials-based diagnostics have tremendous advantages over other conventional diagnostic systems in terms of sensitivity, specificity, and portability owing to the unique intrinsic properties of nanomaterials. Thus, there is a substantial need to develop and employ a broad spectrum of nanomaterials for biological and diagnostic applications. In this review, we focus on nanomaterials-assisted disease diagnosis utilizing different techniques such as photoluminescence, colorimetry, fluorescence, electrochemistry, microarray methods, surface plasmon resonance, and surface-enhanced Raman spectroscopy. In these techniques, different nanomaterials, including but not limited to gold and silver materials, metal oxides, and semiconductors, were employed according to their all-purpose chemical and physical properties. As the new properties of nanomaterials are discovered, their contributions to nanobiotechnology applications are vastly increased. In this review, the features of the selected nanobiomaterials, biological tag-modified nanomaterials, and their outstanding applications for the detection of specific biotargets have been summarized according to the latest studies. Nanomaterials contribute to the fields of photo/chemotherapy and biomedical imaging in particular, since all the biological events happen within this size range and beneficiary roles of nanoparticles (NPs) are countless in biomedical applications due to their unique physical and chemical properties. However, due to the necessity of specificity and selectivity, there is a clear ambition to study bioconjugation of NPs to increase the efficiency of the targeted biological applications. Thus, the combination of the specificity and the intrinsic properties of biohybrid NPs facilitate diagnostic and therapeutic applications.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":"41 1","pages":"673-699"},"PeriodicalIF":2.2,"publicationDate":"2017-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/BIY-1705-31","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49237549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Targeted delivery of etoposide to osteosarcoma cells using poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanoparticles 使用聚(3-羟基丁酸酯-co-3-羟基戊酸酯)(PHBV)纳米颗粒向骨肉瘤细胞靶向递送依托泊苷
IF 2.2 4区 生物学 Q3 BIOLOGY Pub Date : 2017-10-01 DOI: 10.3906/BIY-1612-17
E. Alp, T. Çırak, M. Demirbilek, M. Türk, Eylem Güven
Folic acid (FA)-functionalized poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanoparticles were prepared to enhance the delivery efficiency of the anticancer drug etoposide for the clinical treatment of osteosarcoma. PHBV nanoparticles were synthesized by emulsification/solvent evaporation technique and obtained in the size range of 200-250 nm and zeta potential range of -21 and -27 mV. Encapsulation efficiency and in vitro drug release were studied. The cytotoxic, apoptotic, and necrotic effects of PHBV nanoparticles were also investigated using Saos-2 osteosarcoma cells. The results obtained in this study demonstrate that etoposide-loaded and FA-functionalized PHBV nanoparticles can be successfully used for targeted treatment of osteosarcoma.
制备了叶酸(FA)功能化聚(3-羟基丁酸-co-3-羟基戊酸)(PHBV)纳米颗粒,以提高抗癌药物依托泊苷的递送效率,用于骨肉瘤的临床治疗。采用乳化/溶剂蒸发法制备了PHBV纳米颗粒,粒径范围为200 ~ 250 nm, zeta电位范围为-21和-27 mV。考察其包封率和体外释药效果。我们还利用Saos-2骨肉瘤细胞研究了PHBV纳米颗粒的细胞毒性、凋亡和坏死作用。本研究结果表明,依托泊苷负载和fa功能化的PHBV纳米颗粒可以成功用于骨肉瘤的靶向治疗。
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引用次数: 7
Accumulation of phenolics in natural and micropropagated plantlets of Thymus pseudopulegioides Klokov & Des.-Shost. with their antioxidant potentials 人工和微繁殖胸腺植株中酚类物质的积累-Shost。具有抗氧化潜力
IF 2.2 4区 生物学 Q3 BIOLOGY Pub Date : 2017-10-01 DOI: 10.3906/BIY-1704-9
M. Günaydin, A. Laghari, E. Bektaş, M. Sökmen, A. Sokmen
Thymus pseudopulegioides plantlets were propagated in vitro via direct organogenesis by using Murashige and Skoog (MS) media containing kinetin, thidiazuron, and 6-benzyladenine (BA) individually. Methanol extracts obtained both from plantlets and wild plants were analyzed for their total phenolics and flavonoid contents, then quantified by HPLC. The highest total phenolic (8.83 mg/g as gallic acid equivalent) and total flavonoid (0.92 mg/mL as rutin equivalent) values were from the MS media supplemented with 1.0 mg/L kinetin and 0.5 mg/L BA, respectively. The plantlets grown in those media also showed remarkable antioxidant activities with an IC50 value of 4.77 mug/mL in DPPH and 100% inhibition in s-carotene assays, respectively. HPLC analysis proved the production of protocatechuic, caffeic, vanillic, rosmarinic, ferulic, and o-coumaric acids and rutin. Rosmarinic acid production was predominant in natural samples (115.2 mg/100 g dry weight), while the aforesaid phenolic acids were prevalent in plantlets grown on MS media supplemented with KIN or BA at various concentrations. Rutin production was the highest (50.74 mg/100 g dry weight) in the plantlets grown on MS medium containing kinetin (1.0 mg/L). As an economically important chemical, rosmarinic acid was selected as the target chemical and a novel method was introduced to achieve its selective isolation.
采用Murashige和Skoog(MS)培养基,分别含有激动素、噻二唑仑和6-苄基腺嘌呤(BA),通过直接器官发生法在体外繁殖拟蒲胸腺植株。对从植株和野生植物中获得的甲醇提取物进行总酚和类黄酮含量分析,然后用HPLC定量。最高的总酚(以没食子酸当量计为8.83mg/g)和总黄酮(以芦丁当量计为0.92mg/mL)值分别来自补充1.0mg/L激动素和0.5mg/L BA的MS培养基。在这些培养基中生长的植株也表现出显著的抗氧化活性,在DPPH中的IC50值分别为4.77μg/mL,在s-胡萝卜素测定中的抑制率分别为100%。高效液相色谱分析证实了原儿茶酸、咖啡酸、香兰素、迷迭香酸、阿魏酸、邻香豆酸和芦丁的产生。迷迭香酸的产生在天然样品中占主导地位(115.2mg/100g干重),而上述酚酸在用不同浓度的KIN或BA补充的MS培养基上生长的植株中普遍存在。在含有激动素(1.0mg/L)的MS培养基上生长的植株中,芦丁产量最高(50.74mg/100g干重)。作为一种经济上重要的化学物质,迷迭香酸被选为目标化学物质,并介绍了一种新的方法来实现其选择性分离。
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引用次数: 4
In vitro evaluation of PLLA/PBS sponges as a promisingbiodegradable scaffold for neural tissue engineering 聚乳酸/PBS海绵作为神经组织工程生物降解支架的体外评价
IF 2.2 4区 生物学 Q3 BIOLOGY Pub Date : 2017-10-01 DOI: 10.3906/BIY-1701-6
İrem Ayşe Kanneci Altinişik, F. N. Kok, D. Yucel, G. Kose
In tissue engineering, the use of poly-L-lactic acid (PLLA)/polybutylene succinate (PBS) blend for the construction of scaffold is very limited. Moreover, polymeric sponges fabricated from PLLA/PBS have not been studied for neural tissue engineering. In the present study, the potential of the utility of PLLA/PBS polymeric sponges seeded with Schwann cells was investigated. PLLA and PBS were blended in order to increase the processability and tune the crystallinity, porosity, and degradation rate of the resulted polymeric sponges. These sponges were then seeded with Schwann cells. Porosity analysis showed that there were no significant differences between different compositions of PLLA/PBS blends; however, the porosity was slightly higher in PLLA/PBS (3%, w/v, 2:1) scaffold. Degradation profiles were also investigated for 120 days and almost 25% weight of PLLA/PBS (6%, 4%, 2%, w/v, 1:1) scaffolds and 18% weight of PLLA/PBS (3%, w/v, 2:1) scaffolds were lost at the end of 120 days. In vitro cell culture studies were also performed and the results proved that all PLLA/PBS blended scaffolds were biocompatible. The highest cell proliferation was observed for PLLA/PBS (3%, w/v, 2:1) scaffolds and this construct can be considered a promising biodegradable scaffold for neural tissue engineering.
在组织工程中,聚-L-乳酸(PLLA)/聚丁二酸丁二醇酯(PBS)共混物用于构建支架的用途非常有限。此外,由PLLA/PBS制备的聚合物海绵还没有被研究用于神经组织工程。在本研究中,研究了植入施旺细胞的PLLA/PBS聚合物海绵的应用潜力。将PLLA和PBS共混以提高可加工性并调节所得聚合物海绵的结晶度、孔隙率和降解率。然后将这些海绵接种施旺细胞。孔隙率分析表明,PLLA/PBS共混物的不同组成之间没有显著差异;然而,PLLA/PBS(3%,w/v,2:1)支架中的孔隙率略高。还研究了120天的降解情况,在120天结束时,几乎25%重量的PLLA/PBS(6%,4%,2%,w/v,1:1)支架和18%重量的PLLA/PBS(3%,w/v:2:1)支架损失。还进行了体外细胞培养研究,结果证明所有PLLA/PBS混合支架都具有生物相容性。在PLLA/PBS(3%,w/v,2:1)支架中观察到最高的细胞增殖,并且该构建体可以被认为是用于神经组织工程的有前途的可生物降解支架。
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引用次数: 4
Role of alternative phosphorylation and O-glycosylation of erythropoietinreceptor in modulating its function: an in silico study 促红细胞生成素受体的选择性磷酸化和O-糖基化在调节其功能中的作用:一项计算机研究
IF 2.2 4区 生物学 Q3 BIOLOGY Pub Date : 2017-08-02 DOI: 10.3906/biy-1704-3
H. Kausar, Sana Gull, Waqar Ahmad, S. Awan, M. Sarwar, B. Ijaz, M. Ansar, S. Asad, S. Hassan
Detailed knowledge of the three-dimensional (3D) structure of a protein is essential for the proper understanding of its function(s) that could be modified through posttranslational modifications (PTMs). Among these PTMs, alterations of serine/threonine residues of a protein through phosphorylation and O-glycosylation are extremely dynamic and could modulate the functions of a protein by affecting their 3D structure. Potential of a protein for certain PTMs could be evaluated through computer-based methods. Erythropoietin (EPO) is a multifunctional protein that primarily regulates red blood cell production and is also involved in other nonhematopoietic functions; for example, EPO also has cardioprotective and neuroprotective effects. In this study, multifunctional EPO behavior has been revealed based on transient modifications of its receptor. In this study, PTMs of erythropoietin receptor (EPO-R) were predicted using neural network tools, and the possible effects of these modifications are suggested. Phosphorylation and O-glycosylation at serine 380 and 444 of the cytoplasmic domain of EPO-R seem to have an antagonistic role in controlling signaling events induced by EPO. O-glycosylation at threonine 423 might hinder beta-TrCP (a ubiquitin ligase) binding, which ubiquitinates at K 428, and ultimately results in the recycling of EPO-R, thus increasing EPO sensitivity. In contrast, the phosphorylated form of the same residue inhibits the recycling of EPO-R and thereby decreases the EPO sensitivity. Additionally, the interplay of O-glycosylation modification at serine 478 and phosphorylation at tyrosine 479 might help in controlling the duration of EPO-induced signaling.
对蛋白质三维(3D)结构的详细了解对于正确理解其可以通过翻译后修饰(PTM)修饰的功能至关重要。在这些PTM中,蛋白质的丝氨酸/苏氨酸残基通过磷酸化和O-糖基化的改变是非常动态的,并且可以通过影响其3D结构来调节蛋白质的功能。蛋白质对某些PTM的潜力可以通过基于计算机的方法进行评估。促红细胞生成素(EPO)是一种多功能蛋白,主要调节红细胞的产生,也参与其他非造血功能;例如EPO也具有心脏保护和神经保护作用。在这项研究中,基于其受体的瞬时修饰,揭示了多功能EPO的行为。在本研究中,使用神经网络工具预测了红细胞生成素受体(EPO-R)的PTM,并提出了这些修饰的可能效果。EPO-R细胞质结构域丝氨酸380和444的磷酸化和O-糖基化似乎在控制EPO诱导的信号事件中具有拮抗作用。苏氨酸423处的O-糖基化可能阻碍β-TrCP(一种泛素连接酶)结合,其在K 428处泛素化,并最终导致EPO-R的再循环,从而增加EPO的敏感性。相反,相同残基的磷酸化形式抑制EPO-R的再循环,从而降低EPO的敏感性。此外,丝氨酸478处的O-糖基化修饰和酪氨酸479处的磷酸化的相互作用可能有助于控制EPO诱导的信号传导的持续时间。
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引用次数: 1
期刊
Turkish Journal of Biology
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