Pub Date : 2015-04-01DOI: 10.17533/UDEA.VITAE.V22N1A04
Yenny Bueno-Duarte, S. Mendez-Sánchez
Bacground: The linalool (2,6-dimethyl-2 ,7-octadiene-6-ol) is a monoterpene present in more than 200 types of plants, and it is the major component of several essential oils. The linalool has a wide range of potential pharmacological properties, such as antiseptic, anxiolytic, anti-inflammatory, antifungal, antimicrobial and proapoptotic in different tumor cell lines. However, its primary use is associated as an odorant molecule or fragrance in topical products, in cosmetics products and fragrance industry. Objective: In this study the effect of linalool on the bioenergetics of isolated rat liver mitochondria was evaluated. Methods: Linalool in concentrations between (5-100μg/mL) was evaluated in isolated mitochondria of male albino Wistar rats, according to the method described by Voss et al (1961). Initially, the oxygen consumption by intact mitochondria was determined by polarographic method using an oxygraph. Subsequently, the effect of the compound on mitochondrial respiratory chain enzymes was evaluated, by using polarographic and spectrophotometric methods. Results: The results obtained indicated that linalool, at concentrations of 5, 10, 50 and 100μg/mL, affects the rate of oxygen consumption in state 3 and 4 of mitochondria energized with glutamate-malate and succinate. However, the inhibitory effect was greater when using glutamate-malate as substrate. For its part, the analysis of the enzyme activities of the mitochondrial respiratory chain showed that linalool (50μg/mL) inhibits complex I, III, and ATPase activity. Conclusions: linalool inhibits complex I, complex III and ATPase, this inhibition could increase the generation of reactive oxygen species.
背景:芳樟醇(2,6-二甲基- 2,7 -辛二烯-6-醇)是一种单萜，存在于200多种植物中，是几种精油的主要成分。芳樟醇在不同肿瘤细胞系中具有抗菌、抗焦虑、抗炎、抗真菌、抗菌和促凋亡等潜在药理作用。然而，它的主要用途是作为一种气味分子或香水在局部产品，化妆品和香水行业。目的:研究芳樟醇对离体大鼠肝脏线粒体生物能量学的影响。方法:根据Voss et al(1961)的方法，测定雄性白化Wistar大鼠离体线粒体中浓度为(5 ~ 100μg/mL)的芳樟醇。最初，完整线粒体的耗氧量是用极谱法测定的。随后，用极谱法和分光光度法评价了该化合物对线粒体呼吸链酶的影响。结果:5、10、50和100μg/mL浓度的芳樟醇对谷氨酸-苹果酸和琥珀酸激活线粒体状态3和4的耗氧速率有影响。而以谷氨酸-苹果酸盐为底物时，抑制作用更大。对线粒体呼吸链酶活性分析表明，芳樟醇(50μg/mL)抑制复合体I、III和atp酶活性。结论:芳樟醇抑制复合体I、复合体III和atp酶，这种抑制作用可增加活性氧的生成。
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Pub Date : 2015-01-01DOI: 10.17533/UDEA.VITAE.V22N3A01
P. Mastroianni, F. Varallo
The prevalence of hospital admissions because of possible adverse drug events (ADEs) vary from 0.56% to 54.5% in Brazil. In addition, nearly 43% of inpatients may develop adverse drug reactions (ADRs) during their hospital stays. Clinical outcomes arising from drug-related problems are almost never identified by health professionals because the signs and symptoms are nonspecific and may mimic pathologies.
{"title":"Risk factors for early prediction of adverse drug events related to hospitalizations","authors":"P. Mastroianni, F. Varallo","doi":"10.17533/UDEA.VITAE.V22N3A01","DOIUrl":"https://doi.org/10.17533/UDEA.VITAE.V22N3A01","url":null,"abstract":"The prevalence of hospital admissions because of possible adverse drug events (ADEs) vary from 0.56% to 54.5% in Brazil. In addition, nearly 43% of inpatients may develop adverse drug reactions (ADRs) during their hospital stays. Clinical outcomes arising from drug-related problems are almost never identified by health professionals because the signs and symptoms are nonspecific and may mimic pathologies.","PeriodicalId":23515,"journal":{"name":"Vitae-revista De La Facultad De Quimica Farmaceutica","volume":"265 1","pages":"175-176"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90766396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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