Veterinary microbiology最新文献_第8页

Genetic characteristics of three duck-original H1N1 influenza A viruses isolated in China 中国分离的三种鸭源甲型H1N1流感病毒的遗传特征

IF 2.7 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology

Pub Date : 2025-11-19 DOI: 10.1016/j.vetmic.2025.110813

Qiuqi Kan , Xuefeng Yin , Xingyao Guo , Xinran Chu , Jianjun Sang , Xudong Cao , Jixiang Wang , Wenjie Jiang , Zhehong Zhao , Quan Xie , Tuofan Li , Hongxia Shao , Aijian Qin , Zhimin Wan , Jianqiang Ye

Waterfowl play a pivotal role in the evolution of the influenza A virus (IAV), which harbor an extensive repertoire of IAV and act as long-term reservoirs in which low-pathogenic strains can reassort and acquire new genomic segments. In this study, three H1N1 IAVs, named as A/duck/Jiangxi/DP771/2021(H1N1) (DP771), A/duck/DP1433/2021(H1N1) (DP1433), and A/duck/Jiangxi/DP1467/2021(H1N1) (DP1467), were isolated from wild ducks and characterized. Phylogenetic analysis revealed that all these three viruses belonged to Eurasian lineages. In vitro, all these three isolates replicated efficiently in both A549 and MDCK cells, and demonstrated dual receptor binding properties (α2,3- and α2,6-linked sialic acids). Most strikingly, the mouse study showed that two of these three H1N1 viruses, DP1433 and DP1467, replicated efficiently in lungs without pre-adaptation, and DP1433 caused about 20 % bodyweight loss in mice. All these data provide valuable insight into the molecular epidemiology and pathogenicity of duck-original H1N1 viruses circulating in China, and continued surveillance to monitor the diversity of IAVs in ducks is critical to understand the natural history of IAVs and develop efficient strategies against IAVs.

水禽在甲型流感病毒（IAV）的进化中起着关键作用，它拥有广泛的IAV库，并作为低致病性毒株可以重新组合并获得新的基因组片段的长期宿主。本研究从野鸭中分离出3株H1N1病毒，分别命名为A/duck/江西/DP771/2021(H1N1) （DP771）、A/duck/DP1433/2021(H1N1) （DP1433）和A/duck/江西/DP1467/2021(H1N1) （DP1467）。系统发育分析显示这三种病毒都属于欧亚谱系。在体外，这三种分离株均能在A549和MDCK细胞中高效复制，并表现出双受体结合特性（α2,3-和α2,6-链唾液酸）。最引人注目的是，小鼠研究表明，这三种H1N1病毒中的两种，DP1433和DP1467，在没有预适应的情况下在肺部有效地复制，DP1433导致小鼠体重减轻约20% %。这些数据为了解在中国流行的鸭源H1N1病毒的分子流行病学和致病性提供了有价值的见解，对鸭源禽流感病毒多样性的持续监测对于了解禽流感病毒的自然历史和制定有效的应对策略至关重要。

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引用次数: 0

Antimicrobial susceptibility and genomic determinants of resistance and virulence in Mycoplasma cynos and Mycoplasma felis cynos支原体和felis支原体的抗菌素敏感性和耐药性和毒力的基因组决定因素

IF 2.7 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology

Pub Date : 2025-11-18 DOI: 10.1016/j.vetmic.2025.110808

Isaac Framst , Michael L. Beeton , Shelley W. Peterson , Irene Martin , Jeff L. Caswell , Grazieli Maboni

Mycoplasma cynos and Mycoplasma felis are important respiratory pathogens in dogs and cats. Due to the challenges of culturing these fastidious bacteria, little is known about their antimicrobial susceptibility or mechanisms of pathogenicity. Treatment is typically empirical, as in vitro antimicrobial activity has not been evaluated, and therapeutic efficacy remains unclear. This study aimed to assess in vitro susceptibility and identify genetic markers of antimicrobial resistance (AMR) and virulence in M. cynos and M. felis clinical isolates. Minimum inhibitory concentrations (MICs) for doxycycline, tetracycline, minocycline, enrofloxacin, marbofloxacin, and azithromycin were determined using a broth microdilution assay developed for this study. Hybrid genomes were generated using Oxford Nanopore and Illumina sequencing. AMR-associated single-nucleotide polymorphisms (SNPs) in the gyrA gene correlated with high MICs to enrofloxacin and marbofloxacin in both species. Mutations in 23S rRNA were associated with reduced susceptibility to azithromycin. In M. felis, novel variants in gyrA and the 50S ribosomal protein L4 were linked to decreased susceptibility to fluoroquinolones and azithromycin, respectively. The data also suggest potential intrinsic resistance to azithromycin in M. felis. Low MICs were observed for tetracyclines, and resistance mutations were not identified in the 16S rRNA gene, supporting tetracyclines as effective first-line treatment options. Virulence genes, particularly those associated with adhesion and immune evasion, were detected in both M. cynos and M. felis. This study presents the first comprehensive genomic and phenotypic analysis of AMR and virulence in M. cynos and M. felis, providing new insights into their pathogenicity and informing evidence-based therapeutic strategies.

犬支原体和猫支原体是犬和猫重要的呼吸道病原体。由于培养这些挑剔的细菌的挑战，对它们的抗菌敏感性或致病性机制知之甚少。治疗通常是经验性的，因为体外抗菌活性尚未得到评估，治疗效果仍不清楚。本研究旨在评估cynos和M. felis临床分离株的体外敏感性，并鉴定其抗微生物药物耐药性和毒力的遗传标记。对强力霉素、四环素、米诺环素、恩诺沙星、马布沙星和阿奇霉素的最低抑制浓度（mic）采用为本研究开发的肉汤微量稀释法测定。使用Oxford Nanopore和Illumina测序生成杂交基因组。gyrA基因中amr相关的单核苷酸多态性（snp）与两个物种对恩诺沙星和马布沙星的高mic相关。23S rRNA突变与阿奇霉素易感性降低有关。在猫科动物中，gyrA和50S核糖体蛋白L4的新变异分别与氟喹诺酮类药物和阿奇霉素的易感性降低有关。这些数据还表明，猫分枝杆菌对阿奇霉素有潜在的内在耐药性。观察到四环素类药物的低mic，并且在16S rRNA基因中未发现耐药突变，支持四环素类药物作为有效的一线治疗选择。毒力基因，特别是与黏附和免疫逃避相关的基因，在犬和猫中都被检测到。本研究首次对cynos和M. felis的AMR和毒力进行了全面的基因组和表型分析，为它们的致病性提供了新的见解，并为循证治疗策略提供了信息。

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引用次数: 0

Identification and phylogenetic analysis of a Brucella canis Isolate from an aborted canine fetus 犬流产胎儿分离布鲁氏菌的鉴定及系统发育分析。

IF 2.7 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology

Pub Date : 2025-11-17 DOI: 10.1016/j.vetmic.2025.110810

Fengzekuan Zhao , Qiuju Yang , Zhiguo Liu , Zhenjun Li , Xiaoan Cao

Brucella canis is an important veterinary pathogen with growing public health concerns, but it remains neglected. In this study, a B. canis strain (BMNM19) was characterized using bacteriological culture, biotyping, whole-genome sequencing single nucleotide polymorphism analysis (WGS-SNP). Bacteriological examination revealed rough, grayish-white, sticky, dry, and opaque colonies, and conventional biotyping confirmed it as B. canis, which posing risks to humans in contact with infected dogs. Genomic analysis showed that BMNM19 shares 99.99 % average nucleotide identity (ANI) with the reference strain B. canis ATCC 23365. The strain was found to carry an mprF resistance gene and 69 virulence-associated genes, shared 2829 core genes with other reference strains, and displayed no unique genes. In silico MLST assigned BMNM19 to sequence type ST21. Phylogenetic based on core genome SNPs revealed that BMNM19 is closely related to B. canis strains from Zhejiang, Jiangsu Province (China), Japan and South Korea. These results expand our understanding of the genetic diversity of B. canis in northern, China and emphasize its persistent zoonotic threat to dogs and humans in close contact. This study provides critical molecular insights into the epidemiology of B. canis, to underscore the need for strengthened public health surveillance of this emerging zoonotic pathogen.

犬布鲁氏菌是一种重要的兽医病原体，引起了越来越多的公共卫生关注，但它仍然被忽视。本研究采用细菌学培养、生物分型、全基因组测序单核苷酸多态性分析（WGS-SNP）对一株犬B. bbmm19菌株进行了鉴定。细菌学检查显示菌落粗糙、灰白色、粘稠、干燥和不透明，常规生物分型证实为犬b型，对接触受感染犬的人类构成风险。基因组分析表明，BMNM19与参考菌株ATCC 23365具有99.99 %的平均核苷酸同源性（ANI）。该菌株携带1个mprF抗性基因和69个毒力相关基因，与其他参比菌株共有2829个核心基因，无独特基因。在硅中，MLST将BMNM19分配给序列类型ST21。基于核心基因组snp的系统发育分析显示，BMNM19与浙江、江苏、日本和韩国的犬B. canis菌株亲缘关系较近。这些结果扩大了我们对中国北方犬B. canis遗传多样性的认识，并强调了其对密切接触的狗和人的持续人畜共患威胁。本研究为犬双头绦虫的流行病学提供了重要的分子见解，强调了加强对这种新出现的人畜共患病原体的公共卫生监测的必要性。

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引用次数: 0

CRISPR/Cas9-generated CD46-knockout spermatogonial stem cells reveal mechanisms of BVDV-induced reproductive dysfunction in male livestock CRISPR/ cas9产生的敲除cd46的精原干细胞揭示了bvdv诱导雄性牲畜生殖功能障碍的机制

IF 2.7 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology

Pub Date : 2025-11-16 DOI: 10.1016/j.vetmic.2025.110807

Jiannan Li , Xueting Li , Xiao Li , Wenjie Xu , Liming Yuan , Hongzhao Shi , Zuo Lei , Na Li , Yulei Wei , Jinlian Hua

Bovine viral diarrhea virus (BVDV) is a major viral pathogen that affects ruminants, resulting in significant economic losses due to issues such as immunosuppression, reproductive disorders, and growth retardation. Bulls infected with this virus may become infertile within a few months and can transmit the virus to susceptible cattle during mating. However, the mechanism of BVDV impairing the reproductive function of male livestock is not clear, as there is no suitable cell model. This study used spermatogonial stem cells(SSCs) from cattle and goats as research materials to explore the mechanism by which BVDV affects the reproductive function of male livestock. The results of this study indicate that both cytopathic (cp) and noncytopathic (ncp) BVDV can replicate in SSCs and that SSCs are capable of producing infectious BVDV. Giemsa staining showed significant changes in the morphology of SSCs after BVDV infection. Western blot and mRNA analysis showed that proliferation-related genes (PCNA, CCND1, CDK2) and SSC functional genes (Lin28A, OCT4, SOX2) were down regulated after infection. In addition, BVDV infection can induce ferroptosis in SSCs. Furthermore, CRISPR-Cas9 mediated editing of CD46 in goat SSCs resulted in a decrease in BVDV infection rate and alleviated the negative impact of the virus on cell survival and proliferation. This study provides new insights into the mechanism of reduced reproductive function in male livestock infected with BVDV, and lays the foundation for developing targeted disease resistant breeding strategies.

牛病毒性腹泻病毒（Bovine viral diarrhea virus， BVDV）是一种影响反刍动物的主要病毒性病原体，由于免疫抑制、生殖障碍和生长迟缓等问题而造成重大经济损失。感染这种病毒的公牛可能在几个月内不育，并可在交配期间将病毒传染给易感的牛。然而，由于没有合适的细胞模型，BVDV损害雄性牲畜生殖功能的机制尚不清楚。本研究以牛、山羊精原干细胞（SSCs）为研究材料，探讨BVDV对雄性牲畜生殖功能的影响机制。本研究结果表明，细胞病变（cp）和非细胞病变（ncp） BVDV都可以在SSCs中复制，并且SSCs能够产生感染性BVDV。吉姆萨染色显示BVDV感染后SSCs形态发生显著变化。Western blot和mRNA分析显示，感染后增殖相关基因PCNA、CCND1、CDK2和SSC功能基因Lin28A、OCT4、SOX2表达下调。此外，BVDV感染可诱导ssc铁下垂。此外，CRISPR-Cas9介导的山羊ssc中CD46的编辑导致BVDV感染率下降，减轻了病毒对细胞存活和增殖的负面影响。本研究为研究BVDV感染雄性家畜生殖功能下降的机制提供了新的思路，为制定针对性的抗病育种策略奠定了基础。

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引用次数: 0

Identification and evaluation of novel antigens PykA, CPE1060 and Mbp as G-type Clostridium perfringens subunit vaccines 新型抗原PykA、CPE1060和Mbp作为g型产气荚膜梭菌亚单位疫苗的鉴定与评价。

IF 2.7 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology

Pub Date : 2025-11-14 DOI: 10.1016/j.vetmic.2025.110806

Yifei Chen , Zewei Li , Haiping Xie , Quan Li , Huoying Shi

Necrotic enteritis (NE) is a multifactorial intestinal disease in broilers caused by Clostridium perfringens (C. perfringens) and poses a substantial economic threat to the global poultry industry. There is an urgent need for effective control methods. Vaccination is an effective method for controlling NE infections. Screening and identification of new protective antigen candidates are of significant importance. In this study, three new C. perfringens candidate antigens， pyruvate kinase (PykA), hypothetical protein CPE1060 (CPE1060), and maltose ABC transporter substrate-binding protein (Mbp), which were identified based on immunoproteomics in the previous study, were evaluated for antigenicity, immunogenicity, and induced immune protection efficiency. The results showed that all three candidate antigens possessed good immunogenicity and antigenicity, could induce high levels of humoral and cellular immune responses, and could significantly reduce intestinal damage caused by Clostridium perfringens infections in chickens. Among them, the protective effects of CPE1060 and Mbp proteins as subunit vaccines were superior to those of PykA proteins. This study may provide new insights into the prevention and control of NE.

坏死性肠炎（NE）是由产气荚膜梭菌（C. perfringens）引起的肉鸡多因子肠道疾病，对全球家禽业构成重大经济威胁。迫切需要有效的控制方法。疫苗接种是控制NE感染的有效方法。筛选和鉴定新的保护性抗原候选物具有重要意义。本研究利用免疫蛋白质组学方法鉴定了3种新的产气荚膜荚膜菌候选抗原pyruvate kinase （PykA）、假设蛋白CPE1060 （CPE1060）和麦芽糖ABC转运蛋白底物结合蛋白（Mbp），并对其抗原性、免疫原性和诱导免疫保护效率进行了评价。结果表明，3种候选抗原均具有良好的免疫原性和抗原性，能诱导高水平的体液免疫和细胞免疫反应，并能显著减轻产气荚膜梭菌感染对鸡肠道的损伤。其中，CPE1060和Mbp蛋白作为亚单位疫苗的保护作用优于PykA蛋白。本研究为NE的防治提供了新的思路。

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引用次数: 0

Porcine reproductive and respiratory syndrome virus nsp6 hijacks ATP1B1 antagonizing TRAF6 mediated antiviral innate immunity 猪繁殖与呼吸综合征病毒nsp6劫持ATP1B1，拮抗TRAF6介导的抗病毒先天免疫。

IF 2.7 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology

Pub Date : 2025-11-13 DOI: 10.1016/j.vetmic.2025.110803

Haotian Yang , Han Gu , He Qiu , Bicheng Li , Jidong Xu , Tong Cao , Du Liuyang , Xiaoliang Li , Fang He

Porcine reproductive and respiratory syndrome virus (PRRSV) is an immune-suppressive pathogen that poses a significant challenge to the global swine industry. The mechanism by which PRRSV regulating host inflammation to evade innate immunity remains unclear. Here, Na⁺ /K⁺-ATPase beta1 subunit (ATP1B1), a pivotal antiviral protein, was shown to interact with PRRSV nsp6, a tiny viral protein encoded by ORF1a. ATP1B1 stabilized the protein level of TRAF6 by downregulating K48-linked ubiquitination of TRAF6, thus triggering NF-κB signaling and inflammatory response. Moreover, PRRSV nsp6 competetively interacted with ATP1B1 via the site of Leu 3 and impaired the formation of ATP1B1-TRAF6 complex, leading to TRAF6 proteasomal degradation and compromised inflammatory response. PRRSV with the corresponding mutation in nsp6 L3S was successfully rescued but presented defective virus growth in the late stage of infection, especially under the inflammation condition induced by either ATP1B1 overexpression or poly (I:C) stimulation. In addition, the halt in PRRSV replication was induced by treatment with autophagy inhibitor BafA1 during virus passage. L3S mutant virus impaired the recovery of virus growth even after the removal of BafA1, indicating the key role of nsp6 in sustaining virus vitality under innate immunity. Taken together, these results elucidate the functional mechanism by which PRRSV alleviates the inflammatory response to promote successful virus proliferation and growth recovery from the host innate immune response.

猪繁殖与呼吸综合征病毒（PRRSV）是一种免疫抑制病原体，对全球养猪业构成了重大挑战。PRRSV调节宿主炎症以逃避先天免疫的机制尚不清楚。在这里，Na+ /K+-ATPase beta1亚基（ATP1B1）是一种关键的抗病毒蛋白，被证明与PRRSV nsp6（一种由ORF1a编码的微小病毒蛋白）相互作用。ATP1B1通过下调k48相关的TRAF6泛素化来稳定TRAF6蛋白水平，从而触发NF-κB信号传导和炎症反应。此外，PRRSV nsp6通过Leu 3位点与ATP1B1竞争性相互作用，破坏ATP1B1-TRAF6复合物的形成，导致TRAF6蛋白酶体降解和炎症反应受损。具有相应nsp6 L3S突变的PRRSV成功获救，但在感染后期出现病毒生长缺陷，特别是在ATP1B1过表达或poly （I:C）刺激诱导的炎症条件下。此外，在病毒传代过程中，用自噬抑制剂BafA1处理可诱导PRRSV复制停止。L3S突变病毒即使在去除BafA1后也破坏了病毒生长的恢复，这表明nsp6在先天免疫下维持病毒活力的关键作用。综上所述，这些结果阐明了PRRSV通过减轻炎症反应来促进宿主先天免疫反应中病毒增殖和生长恢复的功能机制。

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引用次数: 0

Neuromedin B and its receptor NMBR inhibit H9N2 infection 神经毒素B及其受体NMBR抑制H9N2感染

IF 2.7 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology

Pub Date : 2025-11-13 DOI: 10.1016/j.vetmic.2025.110790

Guihong Yang , Shimao Tian , Jinyu Yang , Yubing Tang , Ke Tian , Song Wang , Yinli Bao

Neuromedin B (NMB) and its receptor NMBR constitute a neuropeptide system implicated in various physiological processes. While previously associated with innate immunity, their precise antiviral action against influenza A virus (IAV) infection have remained poorly defined. Here, we elucidate the function of the NMB/NMBR axis in the host defense against H9N2 influenza virus. We demonstrate that NMB treatment and NMBR overexpression potentiate IFN-β production and restrict viral replication in H9N2-infected A549 cells and mouse lungs. Conversely, NMBR knockdown compromises the antiviral response, diminishing IFN-β expression and enhancing viral propagation. We further show that NMB/NMBR signaling targets the viral non-structural protein 1 (NS1) by upregulating the E3 ubiquitin ligase TRIM25. Mechanistically, NMB/NMBR activation engages a positive feedback loop with the retinoic acid-inducible gene I (RIG-I) pathway, reinforcing RIG-I activation through enhanced K63-linked ubiquitination while transcriptionally repressing the deubiquitinase CYLD. Consequently, this augmented signaling potentiates the JAK-STAT1 pathway, leading to increased STAT1 phosphorylation and elevated expression of interferon-stimulated gene 15 (ISG15). Our findings establish that the NMB/NMBR axis confers protection against H9N2 IAV by amplifying RIG-I-mediated innate immunity and facilitating NS1 suppression, revealing a pivotal neuroimmune mechanism and suggesting a promising target for developing broad-spectrum, host-directed therapeutics against IAV.

神经肽B （Neuromedin B， NMB）及其受体NMBR构成了一个参与多种生理过程的神经肽系统。虽然以前与先天免疫有关，但它们对甲型流感病毒（IAV）感染的确切抗病毒作用仍然不明确。在此，我们阐明了NMB/NMBR轴在宿主防御H9N2流感病毒中的作用。我们证明NMB处理和NMBR过表达增强了h9n2感染的A549细胞和小鼠肺中IFN-β的产生并限制了病毒的复制。相反，NMBR敲低会损害抗病毒反应，降低IFN-β表达并增强病毒传播。我们进一步发现NMB/NMBR信号通过上调E3泛素连接酶TRIM25靶向病毒非结构蛋白1 （NS1）。在机制上，NMB/NMBR激活与维甲酸诱导基因I （RIG-I）通路形成正反馈回路，通过增强k63相关的泛素化而增强RIG-I激活，同时转录抑制去泛素化酶CYLD。因此，这种增强的信号通路增强了JAK-STAT1通路，导致STAT1磷酸化增加和干扰素刺激基因15 （ISG15）的表达升高。我们的研究结果表明，NMB/NMBR轴通过放大rig - i介导的先天免疫和促进NS1抑制来保护H9N2 IAV，揭示了一个关键的神经免疫机制，并为开发广谱、宿主导向的IAV治疗提供了一个有希望的靶点。

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引用次数: 0

Construction and immunological characterization of two chimeric proteins built from the Mycoplasma bovis genome 牛支原体基因组两种嵌合蛋白的构建及免疫学特性研究

IF 2.7 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology

Pub Date : 2025-11-11 DOI: 10.1016/j.vetmic.2025.110793

Camila Pachêco Gomes , Lucas Santana Coelho da Silva , Bruna Carolina de Brito Guimarães , Manoel Neres Santos Júnior , Maysa Santos Barbosa , Beatriz Almeida Sampaio , Jorge Timenetsky , Bruno Lopes Bastos , Lucas Miranda Marques

Mycoplasma bovis is an important pathogen that affects cattle worldwide. This study aimed to construct and evaluate the antigenicity and immunogenicity of recombinant chimeric proteins containing exclusive M. bovis epitopes. The non-redundant proteomes of M. bovis in UniProt were analyzed using the programs PsortB, TopCons, Cello2GO, BepiPred, LbTope, and IEDB, which resulted in the selection of B-cell epitopes. For the selection of T-cell epitopes, bovine alleles present in IPD were analyzed in NetMHCIIPan with previously selected M. bovis proteins. Using the chosen epitopes, two chimeric proteins (PQ1Mb and PQ2Mb) were constructed, which were expressed in E. coli BL21 Tuner (DE3) induced by Isopropyl β-D-1-tiogalactopiranosídeo (IPTG) at 18°C (PQ1Mb) and in E. coli BL21 (DE3) in auto-inducing medium at 25°C (PQ2Mb), using the pET-28a(+) vector. Antigenicity was confirmed through a Dot blot. Subsequently, Gir breed cows were immunized with the purified proteins and Montanide ISA 61 VG adjuvant, in two doses 30 days apart. The results demonstrate that the proteins induced antibody production. The avidity of the antibodies was also assessed, where the amount required to dissociate 50 % of the antibody in animals after vaccination ranged from 2.5 to 5.4 M of ammonium thiocyanate. Thus, the high specificity of these chimeric antigens suggests their potential for developing an effective vaccine against M. bovis and for improving immunodiagnostic testing.

牛支原体是影响全世界牛的一种重要病原体。本研究旨在构建和评价含有牛牛支原体表位的重组嵌合蛋白的抗原性和免疫原性。使用PsortB、TopCons、Cello2GO、BepiPred、LbTope和IEDB等程序分析牛支原体在UniProt中的非冗余蛋白质组，选择b细胞表位。为了选择t细胞表位，在NetMHCIIPan中使用先前选择的牛支原体蛋白分析了IPD中存在的牛等位基因。利用选择的表位构建两个嵌合蛋白（PQ1Mb和PQ2Mb），分别在异丙基β-D-1-tiogalactopiranosídeo （IPTG）诱导的大肠杆菌BL21 Tuner （DE3）中18°C （PQ1Mb）和在自动诱导培养基中25°C （PQ2Mb）表达，载体为pET-28a（+）。通过Dot blot证实抗原性。随后，用纯化蛋白和Montanide ISA 61 VG佐剂分别接种Gir奶牛，接种时间间隔为30 d。结果表明，这些蛋白诱导了抗体的产生。还对抗体的亲和力进行了评估，在接种疫苗后，动物体内分离50% %抗体所需的量为2.5至5.4 M硫氰酸铵。因此，这些嵌合抗原的高特异性表明它们具有开发有效的牛支原体疫苗和改进免疫诊断测试的潜力。

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引用次数: 0

Fatal septicemia caused by human-associated NDM-5-producing Klebsiella pneumoniae ST323 clone in a dog 由人类相关的产生ndm -5的肺炎克雷伯菌ST323克隆引起的狗致死性败血症。

IF 2.7 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology

Pub Date : 2025-11-11 DOI: 10.1016/j.vetmic.2025.110805

Jéssica Taina Bordin , João Pedro Rueda Furlan , Guilherme E. Paiva , Elder Sano , Bruna Fuga , Rubens R. Sousa-Carmo , Thais Martins-Gonçalves , Andrey Guimarães Sacramento , Nilton Lincopan , Fábio Parra Sellera

Carbapenemase-producing Enterobacterales (CPE), particularly those harboring New Delhi Metallo-β-lactamase (NDM), represent a growing global health threat due to their resistance to carbapenems and other β-lactam antimicrobials. While the presence of NDM-producing Klebsiella pneumoniae is well-documented in human healthcare settings, its emergence in companion animals remains a critical concern. This study reports the identification and genomic characterization of an NDM-5-producing K. pneumoniae strain (ICBKPJB) isolated from an infected dog in Brazil. Strain ICBKPJB exhibited a multidrug resistance profile, including to carbapenems, and carried multiple antimicrobial resistance genes, highlighting bla_NDM-5 and bla_CTX-M-15. Whole-genome sequencing revealed that ICBKPJB belongs to the human healthcare-associated clone of the sequence type (ST) 323, whereas phylogenomic analysis grouped the ICBKPJB strain with human and environmental ST323 strains (SNP counts ranging from 95 to 147). The presence of an IncX3 plasmid harboring the bla_NDM-5 gene was confirmed, whereas in silico plasmid analysis demonstrated that it was closely related to plasmids found in K. pneumoniae strains from both humans and animals worldwide. These findings underscore the risk of interspecies transmission of CPE and emphasize the need to strengthen alliances between human and veterinary medicine to address the emergence, spread, and circulation of carbapenem resistance across both sectors.

产碳青霉烯酶肠杆菌（CPE），特别是那些含有新德里金属β-内酰胺酶（NDM）的肠杆菌，由于对碳青霉烯类和其他β-内酰胺类抗菌剂的耐药性，代表着日益严重的全球健康威胁。虽然在人类卫生保健机构中存在产生ndm的肺炎克雷伯菌，但其在伴侣动物中的出现仍然是一个严重问题。本研究报道了从巴西一只受感染的狗身上分离的一株产ndm -5肺炎克雷伯菌（ICBKPJB）的鉴定和基因组特征。菌株ICBKPJB表现出包括碳青霉烯类在内的多药耐药特征，并携带多种耐药基因，主要是blaNDM-5和blaCTX-M-15。全基因组测序显示，ICBKPJB属于序列型（ST） 323的人类医疗保健相关克隆，而系统基因组分析将ICBKPJB菌株与人类和环境ST323菌株分组（SNP计数从95到147）。证实了含有blaNDM-5基因的IncX3质粒的存在，而硅质粒分析表明，它与全球人类和动物肺炎克雷伯菌菌株中发现的质粒密切相关。这些发现强调了CPE的种间传播风险，并强调需要加强人类和兽医之间的联盟，以解决碳青霉烯类耐药性在这两个部门的出现、传播和循环。

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引用次数: 0

Pathogenicity comparison of NADC34-like porcine reproductive and respiratory syndrome virus in 4-week-old weaned pigs versus 10-week-old growing pigs nadc34样猪繁殖与呼吸综合征病毒对4周龄断奶猪与10周龄生长猪致病性的比较

IF 2.7 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology

Pub Date : 2025-11-11 DOI: 10.1016/j.vetmic.2025.110804

Haemin Jeong , Duri Lee , Kyeng-Cheol Min , An Kook Choi , Hokeun Won , Jieun Chae , Sang-Myeong Lee , Guehwan Jang , Changhee Lee

Porcine reproductive and respiratory syndrome virus (PRRSV) is a globally distributed and financially significant viral pathogen affecting the swine industry. Its extensive genotypic and pathotypic heterogeneity among strains makes disease control challenging. Since first reported in 2022, NADC34-like (lineage 1A, L1A) strains have become predominant in South Korea, attracting substantial attention in the domestic swine industry due to their high pathogenicity and considerable economic impact. This study aimed to explore age-dependent disease severity by comparing the pathogenicity of the Korean NADC34-like PRRSV strain GNU-2353 in 4-week-old (4WO) weaned and 10-week-old (10WO) growing pigs. The 4WO pigs exhibited most of the clinical indicators of virulence, including a high mortality rate (25 %), hyperthermia, reduced average daily weight gain (ADWG), elevated viral loads in various tissues, thymic atrophy, and extensive interstitial pneumonia. Although the 10WO challenge group showed some criteria of virulent infection, such as high fever, low ADWG, and apparent lung lesions, other indicators (viremia, nasal shedding, viral load without mortality, and thymus atrophy) were less severe than those in the 4WO challenge group. Further meta-analysis confirmed that excretion indicators were significantly higher and clinical signs tended to be more severe in 4WO pigs. GNU-2353 infection induced faster and stronger antibody responses in 10WO pigs than in 4WO pigs, while inflammatory cytokine responses were more evident in 4WO pigs. These results showed that pig age influences the outcomes of GNU-2353 infection, mirroring antibody and cytokine responses. These findings provide insights into the age-dependent pathogenesis of NADC34-like viruses for the establishment of better control measures.

猪繁殖与呼吸综合征病毒（PRRSV）是一种影响养猪业的全球分布和经济意义重大的病毒性病原体。其广泛的基因型和病型异质性使疾病控制具有挑战性。自2022年首次报道以来，nadc34样（谱系1A， L1A）菌株已在韩国占主导地位，由于其高致病性和可观的经济影响，引起了国内养猪业的广泛关注。本研究旨在通过比较韩国nadc34样PRRSV菌株GNU-2353在4周龄（4WO）断奶猪和10周龄（10WO）生长猪中的致病性，探讨年龄依赖性疾病严重程度。4WO猪表现出大部分的临床毒力指标，包括高死亡率（25% %）、高热、平均日增重降低（ADWG）、各组织病毒载量升高、胸腺萎缩和广泛间质性肺炎。虽然10WO攻毒组表现出一些毒性感染的标准，如高热、低ADWG和明显的肺部病变，但其他指标（病毒血症、鼻脱落、病毒载量无死亡、胸腺萎缩）没有4WO攻毒组严重。进一步的荟萃分析证实，4WO猪的排泄指标明显更高，临床症状更严重。GNU-2353感染后，10WO猪的抗体反应比4WO猪更快、更强，炎症细胞因子反应在4WO猪中更明显。上述结果表明，猪龄影响GNU-2353感染结局、镜像抗体和细胞因子反应。这些发现为建立更好的控制措施提供了对nadc34样病毒的年龄依赖性发病机制的见解。

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引用次数: 0