Ali Abbas Nikvand, Seyedeh Missagh Jalali, Somayeh Bahrami, Hassan Rahij Torfi
Trypanosoma evansi (T. evansi) is a hemoprotozoan parasite affecting camels and equids, such as horses, mules, and donkeys, and is known to cause surra disease in these animals. Despite the worldwide distribution of T. evansi infections in equids, surra has not been reported in Arabian horses in Khuzestan Province for over 60 years. In September 2018, a 7-year-old Arabian mare was referred from a 10-horse farm in the suburbs of Ahvaz City. The mare presented with a history of weight loss, poor appetite, and proximity to a camel herd. Physical examination revealed a poor body condition score (BCS ≤ 3/9), pale conjunctival and oral mucosal membranes, dehydration, tachycardia, and fever. Other horses in the herd also exhibited gradual weight loss and hyporexia, with two fatalities reported. A hematologic examination of the referred mare showed leukopenia, severe anemia, and thrombocytopenia. Microscopic examination of a whole blood smear confirmed severe infection with Trypanosoma. PCR assessment of a blood sample further confirmed the infection with T. evansi. The referred mare and seven nonreferred patients from the herd received treatment with diminazene diaceturate (5 mg/kg, a single dose) and penicillin for 4 days. Unfortunately, the referred mare died despite the treatment, while the remaining nonreferred horses recovered. This report marks the first molecular diagnosis of surra in an Arabian horse in Khuzestan Province, highlighting the need for further investigation into surra as a re-emerging disease in this significant population of Arabian horses in the region.
{"title":"Clinical occurrence of trypanosomiasis in Arabian horses from Ahvaz","authors":"Ali Abbas Nikvand, Seyedeh Missagh Jalali, Somayeh Bahrami, Hassan Rahij Torfi","doi":"10.1111/vcp.70003","DOIUrl":"10.1111/vcp.70003","url":null,"abstract":"<p><i>Trypanosoma evansi</i> (<i>T. evansi</i>) is a hemoprotozoan parasite affecting camels and equids, such as horses, mules, and donkeys, and is known to cause surra disease in these animals. Despite the worldwide distribution of <i>T. evansi</i> infections in equids, surra has not been reported in Arabian horses in Khuzestan Province for over 60 years. In September 2018, a 7-year-old Arabian mare was referred from a 10-horse farm in the suburbs of Ahvaz City. The mare presented with a history of weight loss, poor appetite, and proximity to a camel herd. Physical examination revealed a poor body condition score (BCS ≤ 3/9), pale conjunctival and oral mucosal membranes, dehydration, tachycardia, and fever. Other horses in the herd also exhibited gradual weight loss and hyporexia, with two fatalities reported. A hematologic examination of the referred mare showed leukopenia, severe anemia, and thrombocytopenia. Microscopic examination of a whole blood smear confirmed severe infection with <i>Trypanosoma</i>. PCR assessment of a blood sample further confirmed the infection with <i>T. evansi</i>. The referred mare and seven nonreferred patients from the herd received treatment with diminazene diaceturate (5 mg/kg, a single dose) and penicillin for 4 days. Unfortunately, the referred mare died despite the treatment, while the remaining nonreferred horses recovered. This report marks the first molecular diagnosis of surra in an Arabian horse in Khuzestan Province, highlighting the need for further investigation into surra as a re-emerging disease in this significant population of Arabian horses in the region.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"186-190"},"PeriodicalIF":1.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Erin E Farrell, Dae Young Kim, Samantha D Crist, Gayle C Johnson, Wendy E Karnia
{"title":"What is your diagnosis? Right frontal lobe mass in a dog.","authors":"Erin E Farrell, Dae Young Kim, Samantha D Crist, Gayle C Johnson, Wendy E Karnia","doi":"10.1111/vcp.70005","DOIUrl":"https://doi.org/10.1111/vcp.70005","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Em Adam, Craig Thompson, Andrea Pires Dos Santos, Joanne Messick
{"title":"What's your diagnosis? Globular to amorphous material in peripheral blood smears.","authors":"Em Adam, Craig Thompson, Andrea Pires Dos Santos, Joanne Messick","doi":"10.1111/vcp.70008","DOIUrl":"https://doi.org/10.1111/vcp.70008","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ioannis L. Oikonomidis, Paul Rees, Jorge Hernando Sanz, Glynn Woods
Background
Hypophosphatemia is commonly observed in unselected human intensive care unit (ICU) patients, and it has been associated, although inconsistently, with worse outcomes and longer duration of hospitalization (DOH). The incidence of hypophosphatemia and its association with mortality and DOH in ICU canine patients is unknown.
Objectives
The aims of this study were to determine the occurrence of hypophosphatemia in unselected ICU canine patients and its association with mortality and DOH.
Methods
The medical records of all dogs admitted to the Teaching Hospital ICU between January 2019 and December 2023 were retrospectively reviewed. Dogs with hypophosphatemia (serum phosphate < 0.9 mmol/L) were identified. For every hypophosphataemic dog included in this study, two age-matched control, non-hypophosphataemic dogs, closely admitted to the ICU in time, were included.
Results
In total, 3233 medical records were reviewed. Hypophosphatemia was noted in ≥ 1 day of hospitalization in 32 dogs (0.99%). The age-matched case and control groups had a median (range) age of 8.0 (1.0–14.0) years. The survival to discharge rates of both hypophosphataemic and control groups were 78% (25/32 and 50/64, respectively), and their DOH (median, 3.5 days; range 1.0–9.0 and median 3.0 days; range 1.0–25.0, respectively) were not significantly different (p = 0.557). Serum phosphate concentration was not correlated with the DOH (p = 0.649).
Conclusions
Hypophosphatemia was noted in only 1% in this canine ICU patient population and was not associated with the survival to discharge and DOH.
{"title":"Hypophosphatemia in Intensive Care Unit Canine Patients: Occurrence and Association With Mortality and Duration of Hospitalization","authors":"Ioannis L. Oikonomidis, Paul Rees, Jorge Hernando Sanz, Glynn Woods","doi":"10.1111/vcp.70013","DOIUrl":"10.1111/vcp.70013","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Hypophosphatemia is commonly observed in unselected human intensive care unit (ICU) patients, and it has been associated, although inconsistently, with worse outcomes and longer duration of hospitalization (DOH). The incidence of hypophosphatemia and its association with mortality and DOH in ICU canine patients is unknown.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The aims of this study were to determine the occurrence of hypophosphatemia in unselected ICU canine patients and its association with mortality and DOH.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The medical records of all dogs admitted to the Teaching Hospital ICU between January 2019 and December 2023 were retrospectively reviewed. Dogs with hypophosphatemia (serum phosphate < 0.9 mmol/L) were identified. For every hypophosphataemic dog included in this study, two age-matched control, non-hypophosphataemic dogs, closely admitted to the ICU in time, were included.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In total, 3233 medical records were reviewed. Hypophosphatemia was noted in ≥ 1 day of hospitalization in 32 dogs (0.99%). The age-matched case and control groups had a median (range) age of 8.0 (1.0–14.0) years. The survival to discharge rates of both hypophosphataemic and control groups were 78% (25/32 and 50/64, respectively), and their DOH (median, 3.5 days; range 1.0–9.0 and median 3.0 days; range 1.0–25.0, respectively) were not significantly different (<i>p</i> = 0.557). Serum phosphate concentration was not correlated with the DOH (<i>p</i> = 0.649).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Hypophosphatemia was noted in only 1% in this canine ICU patient population and was not associated with the survival to discharge and DOH.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"167-170"},"PeriodicalIF":1.2,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lina C. Bilhalva, Leah N. Zebovitz, Felicia B. Nutter, Amanda Higgs, Dewayne Fox, Carolyn Cray, Francisco O. Conrado
Background
The Atlantic sturgeon has faced severe population declines due to overfishing and environmental changes, resulting in its critical conservation status. There is concern today for the survival of this species, leading to the initiation of studies focused on its conservation.
Objectives
The aims of this study were to establish reference intervals (RIs) for plasma protein fractions in Atlantic sturgeon using agarose gel electrophoresis (AGE), to evaluate whether changes occur during the spawning season, and to compare results with those obtained using capillary zone electrophoresis (CZE).
Methods
Between late May and June 2022, blood samples were collected from 81 spawning Atlantic sturgeon. Plasma from all individuals was analyzed using AGE, with a subset of 16 paired samples further evaluated using CZE for comparative analysis.
Results
AGE revealed six distinct protein fractions, while CZE identified seven fractions. Significant differences in the proportions of fraction 3 (traditionally referred to as alpha-1 globulins) were observed between early and late spawning seasons.
Conclusion
Comparison of AGE and CZE results showed that while both methods provided useful data, CZE offered greater resolution and detection sensitivity. Our results provide important data for sturgeon health assessment; however, discrepancies between the two methods underscore the need for method-specific RI.
{"title":"Protein Electrophoresis in the Atlantic Sturgeon (Acipenser oxyrinchus oxyrinchus): Agarose Gel Electrophoresis Reference Intervals, Method Comparison With Capillary Zone Electrophoresis, and Initial Assessment of the Effects of Spawning Season on Protein Fractions","authors":"Lina C. Bilhalva, Leah N. Zebovitz, Felicia B. Nutter, Amanda Higgs, Dewayne Fox, Carolyn Cray, Francisco O. Conrado","doi":"10.1111/vcp.70020","DOIUrl":"10.1111/vcp.70020","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The Atlantic sturgeon has faced severe population declines due to overfishing and environmental changes, resulting in its critical conservation status. There is concern today for the survival of this species, leading to the initiation of studies focused on its conservation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The aims of this study were to establish reference intervals (RIs) for plasma protein fractions in Atlantic sturgeon using agarose gel electrophoresis (AGE), to evaluate whether changes occur during the spawning season, and to compare results with those obtained using capillary zone electrophoresis (CZE).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Between late May and June 2022, blood samples were collected from 81 spawning Atlantic sturgeon. Plasma from all individuals was analyzed using AGE, with a subset of 16 paired samples further evaluated using CZE for comparative analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>AGE revealed six distinct protein fractions, while CZE identified seven fractions. Significant differences in the proportions of fraction 3 (traditionally referred to as alpha-1 globulins) were observed between early and late spawning seasons.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Comparison of AGE and CZE results showed that while both methods provided useful data, CZE offered greater resolution and detection sensitivity. Our results provide important data for sturgeon health assessment; however, discrepancies between the two methods underscore the need for method-specific RI.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"158-166"},"PeriodicalIF":1.2,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Verónica Mato-Martín, Julia Ginders, Barbara Riond, Regina Hofmann-Lehmann, Marilisa Novacco
Scattergram evaluation is an important part of nonstatistical quality control in Veterinary Hematology. In this case report, we present two feline hematological cases with abnormal Sysmex XN-V scattergrams. Case 1 involved a 16-year-old neutered female domestic shorthair cat referred for the investigation of a suspected abdominal mass, which revealed marked mastocythemia on blood smear evaluation. Case 2 involved a 9-year-old castrated male domestic shorthair cat that was presented for follow-up after cystostomy and perineal urethrostomy, which revealed marked basophilia on blood smear evaluation. In both cases, an abnormal population was observed on the white cell differential channel by fluorescence (WDF) scattergram using a Sysmex XN-V hematology analyzer. The abnormal population appeared as an oval cloud of dots between monocytes and eosinophils. Manual re-gating of these unusual populations on the WDF channel correlates with manually determined counts. Mast cells appeared as a lysis-resistant population on the white count and nucleated red blood cell (WNR) scattergram, whereas basophils did not show this feature. Therefore, the appearance of an unexpected population between the eosinophil and monocyte area on the WDF scattergram may indicate mastocythemia or basophilia in cats. Further assessment of the WNR scattergram, focusing on identifying lysis-resistant populations, can help differentiate between basophilia and mastocythemia. However, blood smear evaluation remains mandatory for the accurate enumeration of basophils and/or determination of mastocythemia. Manufacturer software adjustments within the WDF and WNR channels may improve the accuracy of basophil counts in veterinary hematology.
{"title":"Sysmex XN-V scattergrams in feline mastocythemia and basophilia: Two similar but different cases","authors":"Verónica Mato-Martín, Julia Ginders, Barbara Riond, Regina Hofmann-Lehmann, Marilisa Novacco","doi":"10.1111/vcp.70006","DOIUrl":"10.1111/vcp.70006","url":null,"abstract":"<p>Scattergram evaluation is an important part of nonstatistical quality control in Veterinary Hematology. In this case report, we present two feline hematological cases with abnormal Sysmex XN-V scattergrams. Case 1 involved a 16-year-old neutered female domestic shorthair cat referred for the investigation of a suspected abdominal mass, which revealed marked mastocythemia on blood smear evaluation. Case 2 involved a 9-year-old castrated male domestic shorthair cat that was presented for follow-up after cystostomy and perineal urethrostomy, which revealed marked basophilia on blood smear evaluation. In both cases, an abnormal population was observed on the white cell differential channel by fluorescence (WDF) scattergram using a Sysmex XN-V hematology analyzer. The abnormal population appeared as an oval cloud of dots between monocytes and eosinophils. Manual re-gating of these unusual populations on the WDF channel correlates with manually determined counts. Mast cells appeared as a lysis-resistant population on the white count and nucleated red blood cell (WNR) scattergram, whereas basophils did not show this feature. Therefore, the appearance of an unexpected population between the eosinophil and monocyte area on the WDF scattergram may indicate mastocythemia or basophilia in cats. Further assessment of the WNR scattergram, focusing on identifying lysis-resistant populations, can help differentiate between basophilia and mastocythemia. However, blood smear evaluation remains mandatory for the accurate enumeration of basophils and/or determination of mastocythemia. Manufacturer software adjustments within the WDF and WNR channels may improve the accuracy of basophil counts in veterinary hematology.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"87-94"},"PeriodicalIF":1.2,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A closer look at model optimization concepts in artificial intelligence.","authors":"Daniel Castillo","doi":"10.1111/vcp.70000","DOIUrl":"https://doi.org/10.1111/vcp.70000","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jeffrey Brandon, Heather Reider, Kristy L. Pabilonia, A. Russell Moore
Background
The bromocresol green albumin assay (ALBBCG) has been used in birds and reportedly is noncomparable with electrophoretic albumin (ALBPE) in many species. It is accepted for use in some species and rejected in others.
Objectives
We aimed to compare the performance of ALBPE and ALBBCG methods within backyard chickens and compare the performance of ALBBCG in chickens with other veterinary species where the ALBBCG method is accepted and used clinically.
Methods
Chicken plasma collected during reference interval development and samples submitted for diagnostic biochemistry profile were evaluated using the ALBBCG and ALBPE assays. Method comparison was performed according to current recommendations, including the use of Passing–Bablok and Bland–Altman analysis. ALBBCG and ALBPE were also measured in other avian species, dogs, cats, horses, and domestic ruminants. Method comparison was evaluated within and between species, including clinical utility based on the percentage of cases discordantly interpreted as hypo-, normo-, or hyperalbuminemic by ALBBCG and ALBPE.
Results
In chickens, ALBBCG and ALBPE were not comparable, having a constant bias (−0.4 g/dL) and proportional bias. Similarly, the methods were not comparable in other species; > 10% of samples had > TEA (15%) difference in all species. The clinical utility of albumin interpretation in chickens did not differ significantly from that in dogs and horses, as determined by ANOVA.
Conclusions
The data suggest that ALBBCG is not comparable with ALBPE and performs similarly across all tested species. There is no evidence to support the continued rejection of the ALBBCG in chicken and other avians and acceptance in some mammals.
{"title":"Comparison of Electrophoretic and Bromocresol Green Albumin Methods in Chickens and Other Veterinary Species","authors":"Jeffrey Brandon, Heather Reider, Kristy L. Pabilonia, A. Russell Moore","doi":"10.1111/vcp.70015","DOIUrl":"10.1111/vcp.70015","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The bromocresol green albumin assay (ALB<sub>BCG</sub>) has been used in birds and reportedly is noncomparable with electrophoretic albumin (ALB<sub>PE</sub>) in many species. It is accepted for use in some species and rejected in others.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>We aimed to compare the performance of ALB<sub>PE</sub> and ALB<sub>BCG</sub> methods within backyard chickens and compare the performance of ALB<sub>BCG</sub> in chickens with other veterinary species where the ALB<sub>BCG</sub> method is accepted and used clinically.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Chicken plasma collected during reference interval development and samples submitted for diagnostic biochemistry profile were evaluated using the ALB<sub>BCG</sub> and ALB<sub>PE</sub> assays. Method comparison was performed according to current recommendations, including the use of Passing–Bablok and Bland–Altman analysis. ALB<sub>BCG</sub> and ALB<sub>PE</sub> were also measured in other avian species, dogs, cats, horses, and domestic ruminants. Method comparison was evaluated within and between species, including clinical utility based on the percentage of cases discordantly interpreted as hypo-, normo-, or hyperalbuminemic by ALB<sub>BCG</sub> and ALB<sub>PE</sub>.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In chickens, ALB<sub>BCG</sub> and ALB<sub>PE</sub> were not comparable, having a constant bias (−0.4 g/dL) and proportional bias. Similarly, the methods were not comparable in other species; > 10% of samples had > TE<sub>A</sub> (15%) difference in all species. The clinical utility of albumin interpretation in chickens did not differ significantly from that in dogs and horses, as determined by ANOVA.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The data suggest that ALB<sub>BCG</sub> is not comparable with ALB<sub>PE</sub> and performs similarly across all tested species. There is no evidence to support the continued rejection of the ALB<sub>BCG</sub> in chicken and other avians and acceptance in some mammals.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"171-181"},"PeriodicalIF":1.2,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pancreatic lipase assays are commonly utilized in the diagnostic approach to suspected pancreatitis in cats. The Vcheck fPL 2.0 assay is commercially available; however, analytical validation has yet to be reported in the peer-reviewed literature.
Objective
To evaluate the analytic validity of a fluorescent immunoassay for the point-of-care quantification of feline pancreatic lipase immunoreactivity (Vcheck fPL 2.0 assay).
Methods
Linearity was assessed via dilutional parallelism. Intra- and interassay variability was assessed by calculating coefficients of variation (%CV) across replicates on the same day and across multiple days, respectively. Bilirubin, hemoglobin, and intralipid (intralipid 30% IV emulsion, VWR, Radnor, PA, USA) were utilized to evaluate the potential effects of interfering substances on the assay.
Results
Linearity was sub-optimal, with 12/15 diluted runs having an O/E ratio outside of the 80–120% target range. The mean %CV was 10.2% for intra-assay variability and 16.3% for interassay variability. Interfering substances had no significant effect on the results of the Vcheck fPL 2.0 assay (P = 0.888).
Conclusions
The Vcheck fPL 2.0 appears to have poor linearity and suboptimal precision, limiting the accurate interpretation of results. Additional assay optimization should be considered prior to routine clinical use.
{"title":"Analytical Validation of an Automated Point-of-Care Immunoassay for the Measurement of Feline Pancreatic Lipase Immunoreactivity Concentration (Vcheck fPL 2.0)","authors":"Lisa Wetzel, Joerg M. Steiner, Harry Cridge","doi":"10.1111/vcp.70018","DOIUrl":"10.1111/vcp.70018","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Pancreatic lipase assays are commonly utilized in the diagnostic approach to suspected pancreatitis in cats. The Vcheck fPL 2.0 assay is commercially available; however, analytical validation has yet to be reported in the peer-reviewed literature.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To evaluate the analytic validity of a fluorescent immunoassay for the point-of-care quantification of feline pancreatic lipase immunoreactivity (Vcheck fPL 2.0 assay).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Linearity was assessed via dilutional parallelism. Intra- and interassay variability was assessed by calculating coefficients of variation (%CV) across replicates on the same day and across multiple days, respectively. Bilirubin, hemoglobin, and intralipid (intralipid 30% IV emulsion, VWR, Radnor, PA, USA) were utilized to evaluate the potential effects of interfering substances on the assay.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Linearity was sub-optimal, with 12/15 diluted runs having an O/E ratio outside of the 80–120% target range. The mean %CV was 10.2% for intra-assay variability and 16.3% for interassay variability. Interfering substances had no significant effect on the results of the Vcheck fPL 2.0 assay (<i>P</i> = 0.888).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The Vcheck fPL 2.0 appears to have poor linearity and suboptimal precision, limiting the accurate interpretation of results. Additional assay optimization should be considered prior to routine clinical use.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"154-157"},"PeriodicalIF":1.2,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"More than just a “pretty face”","authors":"Robert M. Lukacs","doi":"10.1111/vcp.70007","DOIUrl":"10.1111/vcp.70007","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 1","pages":"5-6"},"PeriodicalIF":1.2,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143804116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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