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Razotkrivanje stanja: istraživanje prevalencije virusa mačje imunodeficijencije i virusa mačje leukemije među mačkama iz skloništa u Portugalu 了解情况:调查葡萄牙猫科动物中猫科免疫缺陷病毒和猫白血病病毒的流行情况
Q3 Veterinary Pub Date : 2024-02-13 DOI: 10.46419/vs.55.5.1
Paulo Afonso, Luís Cardoso, Hélder Quintas, Ana Cláudia Coelho
Feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) are important pathogens affecting feline hosts worldwide, compromising the health, well-being, quality of life, and lifespan of infected cats. This study explores the prevalence of these viral agents among shelter cats in Portugal, aiming to unravel their complexities and implications. The prevalence of FIV and FeLV was assessed by collecting blood samples (n = 326) from cats during medical routine check-ups at shelter admission and testing them with an immunochromatographic kit. The overall seroprevalence of FIV and FeLV was 15.3% (50/326, 95% confidence interval [CI]: 11.6–19.7). The proportion of cats positive to FIV, FeLV, and both FIV and FeLV was 9.8% (32/326, 95% CI: 6.8-13.6), 2.1% (7/326, 95% CI: 0.9-4.4) and 3.4% (11/326, 95% CI: 1.7-6.0), respectively. The prevalence of FIV and both FeLV and FIV appeared to be higher in the adult population. No statistical association was found between sex, breed, hair, or municipality, and FeLV, FIV and both FeLV and FIV prevalence. This is the first report on retroviral prevalence in shelter cats in Portugal, which provides important information regarding FIV and FeLV among stray cats in Portugal, highlighting the need for surveillance of those viruses, improved compliance with vaccination programmes, and vigilant management strategies to limit and control these viruses in the felinepopulation.
猫免疫缺陷病毒(FIV)和猫白血病病毒(FeLV)是影响全球猫科动物宿主的重要病原体,会损害受感染猫的健康、福祉、生活质量和寿命。本研究探讨了这些病毒病原体在葡萄牙收容所猫咪中的流行情况,旨在揭示其复杂性和影响。在收容所收容的猫咪进行常规体检时,收集它们的血液样本(n = 326),然后用免疫层析试剂盒进行检测,从而评估 FIV 和 FeLV 的流行率。FIV和FeLV的总体血清阳性率为15.3%(50/326,95%置信区间[CI]:11.6-19.7)。FIV、FeLV以及FIV和FeLV均呈阳性的猫咪比例分别为9.8%(32/326,95% CI:6.8-13.6)、2.1%(7/326,95% CI:0.9-4.4)和3.4%(11/326,95% CI:1.7-6.0)。FIV以及FeLV和FIV在成年人群中的流行率似乎较高。性别、品种、毛发或城市与 FeLV、FIV 以及 FeLV 和 FIV 的流行率之间没有统计学关联。这是第一份关于葡萄牙收容猫中逆转录病毒流行情况的报告,它提供了有关葡萄牙流浪猫中 FIV 和 FeLV 的重要信息,强调了对这些病毒进行监测、提高对疫苗接种计划的依从性以及采取警惕性管理策略以限制和控制猫群中这些病毒的必要性。
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引用次数: 0
Procjena reproduktivne uspješnosti u koza – pregled odabranih čimbenika koji na to utječu 山羊的繁殖成功率--影响山羊繁殖成功率的特定化学品综述
Q3 Veterinary Pub Date : 2024-02-13 DOI: 10.46419/vs.55.5.8
J. Šavorić, V. Stevanović, S. Vince, Ivona Matić, Juraj Grizelj, Martina Lojkić, Nino Maćešić, M. Samardžija, Tugomir Karadjole, Branimira Špoljarić
Dairy goats are a very successful form of livestock farming in developing countries, with functional products for human consumption, which makes their breeding attractive. Breeding efficiency is determined by successful reproductive parameters. Several parameters can be used as indicators of reproductive success, such as the number of kids born, age at first kidding, kidding interval, percentage of kids born and weaned, abortion rate, body weight of kids at birth and at weaning, and the length of the goat's reproductive life. Due to the seasonality of reproduction and multiple possibilities of controlling the sexual cycle in goats (hormonal and non-hormonal methods), it is difficult to standardise parameters that could be systematically used to detect suboptimal production, and whose improvement would increase reproductive success. Adequate housing conditions and feeding are inseparable from good breeding and productivity of animals. By meeting these measures with constant observation of reproductive success parameters, high fertility, large litters (2 kids per pregnancy) with a survival rate of 90% until weaning can be expected in goat breeding.
在发展中国家,奶山羊是一种非常成功的畜牧业形式,其功能性产品可供人类消费,这使得奶山羊的育种很有吸引力。繁殖效率由成功的繁殖参数决定。有几个参数可作为繁殖成功的指标,如产仔数、初产日龄、产仔间隔、产仔和断奶比例、流产率、出生和断奶时的体重以及山羊的生育期。由于山羊的繁殖具有季节性,而且有多种控制其性周期的方法(激素和非激素方法),因此很难对一些参数进行标准化,这些参数可以系统地用来检测山羊是否达到最佳生产状态,而且改善这些参数可以提高繁殖成功率。适当的饲养条件和饲喂与动物的良好繁殖和生产率密不可分。通过采取这些措施并持续观察繁殖成功率参数,可望在山羊饲养中实现高繁殖率、大产仔数(每胎 2 仔)和 90%的断奶前成活率。
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引用次数: 0
Testing the potential antifungal activity of Origanum vulgare against Aspergillus fumigatus, Aspergillus Niger and Talaromyces marneffei isolated from pets 测试牛至对从宠物身上分离出的烟曲霉、尼日尔曲霉和马内菲他拉菌的潜在抗真菌活性
Q3 Veterinary Pub Date : 2024-02-13 DOI: 10.46419/vs.55.5.6
João Filipe Rama, Ana Cláudia Coelho, Fernanda Maria Leal
Origanum vulgare (oregano) is an aromatic herb commonly used in the Mediterranean, widely known for its medicinal properties. In this experiment, we tested its antifungal properties against two species of Aspergillus (A. niger and A. fumigatus) and the emerging Talaromyces marneffei. We tested the plant’s antifungal activity in a range of concentrations (5, 10, 20 and 30 mg/mL) in which the plant was dissolved and mixed with growth medium (PDA) and then inoculated with the fungi. The percentage of inhibition was measured over 7 days with data being collected on the 3rd, 5th and 7th days. Fungal activity inhibition ranging from 30 to 100% was achieved against Aspergillus niger and Aspergillus fumigatus and from 50 to 100% against Talaromyces marneffei. This multi-disciplinary study brought the use of plants into the veterinary sciences by using samples isolated from pets, with the aim of researching potential alternatives to traditional antifungal treatments and the ultimate goal ofincreasing animal wellbeing.
牛至(Origanum vulgare)是地中海地区常用的芳香草本植物,因其药用特性而广为人知。在本实验中,我们测试了牛至对两种曲霉(黑曲霉和烟曲霉)以及新出现的马内菲酵母菌(Talaromyces marneffei)的抗真菌特性。我们测试了该植物在不同浓度(5、10、20 和 30 mg/mL)下的抗真菌活性,将该植物溶解并与生长培养基(PDA)混合,然后接种真菌。在第 3、5 和 7 天收集数据,在 7 天内测量抑制率。对黑曲霉和烟曲霉的抑制率从 30%到 100%不等,对马拉色菌的抑制率从 50%到 100%不等。这项多学科研究通过使用从宠物身上分离出的样本,将植物的使用带入了兽医学领域,目的是研究传统抗真菌治疗的潜在替代品,最终目标是提高动物的健康水平。
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引用次数: 0
Procjena reproduktivne uspješnosti u koza – pregled odabranih čimbenika koji na to utječu 山羊的繁殖成功率--影响山羊繁殖成功率的特定化学品综述
Q3 Veterinary Pub Date : 2024-02-13 DOI: 10.46419/vs.55.5.8
J. Šavorić, V. Stevanović, S. Vince, Ivona Matić, Juraj Grizelj, Martina Lojkić, Nino Maćešić, M. Samardžija, Tugomir Karadjole, Branimira Špoljarić
Dairy goats are a very successful form of livestock farming in developing countries, with functional products for human consumption, which makes their breeding attractive. Breeding efficiency is determined by successful reproductive parameters. Several parameters can be used as indicators of reproductive success, such as the number of kids born, age at first kidding, kidding interval, percentage of kids born and weaned, abortion rate, body weight of kids at birth and at weaning, and the length of the goat's reproductive life. Due to the seasonality of reproduction and multiple possibilities of controlling the sexual cycle in goats (hormonal and non-hormonal methods), it is difficult to standardise parameters that could be systematically used to detect suboptimal production, and whose improvement would increase reproductive success. Adequate housing conditions and feeding are inseparable from good breeding and productivity of animals. By meeting these measures with constant observation of reproductive success parameters, high fertility, large litters (2 kids per pregnancy) with a survival rate of 90% until weaning can be expected in goat breeding.
在发展中国家,奶山羊是一种非常成功的畜牧业形式,其功能性产品可供人类消费,这使得奶山羊的育种很有吸引力。繁殖效率由成功的繁殖参数决定。有几个参数可作为繁殖成功的指标,如产仔数、初产日龄、产仔间隔、产仔和断奶比例、流产率、出生和断奶时的体重以及山羊的生育期。由于山羊繁殖的季节性和控制性周期的多种可能性(激素和非激素方法),很难对参数进行标准化,这些参数可以系统地用于检测次优生产,改善这些参数可以提高繁殖成功率。适当的饲养条件和饲喂与动物的良好繁殖和生产率密不可分。通过采取这些措施并持续观察繁殖成功率参数,可望在山羊饲养中实现高繁殖率、大产仔数(每胎 2 仔)和 90%的断奶前成活率。
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引用次数: 0
Reproduction mode and Strain effects on Rabbit Breeding Performances 繁殖模式和品系对家兔繁殖性能的影响
Q3 Veterinary Pub Date : 2024-02-13 DOI: 10.46419/vs.55.5.3
Khouloud Dekkiche, Hania Hannani, Achour Menanni, Nassima Ait Issad, D. Khelef, N. Mimoune
This study was carried out to determine the impact of the mode of reproduction and the strain (male and female) of rabbits on some zootechnical and reproduction parameters. For that, monitoring was carried out in 2019 in the region of Setif (North Eastern Algeria) on a semi-intensive breeding of 25 crossbreed rabbits. The statistical analysis showed a very highly significant effect of the mode of reproduction on the number of mating per fertilizing mating (P= 0.0001) with an average of 1 ± 0 in AI (Fertility rate of 80%), while it was of 1.45 ± 0.51 in natural breeding with a fertility rate of 61%. The weight of the rabbits at weaning was higher in AI than in natural mating (587.25 ± 338.19 g vs. 575.0 ± 375.44 g, respectively). The results relating to the number of total born, born alive, weaned rabbits, the farrowing-mating interval and the farrowing-fertilizing mating interval in natural mode were respectively of (7.4 ± 3.12), (4.55 ± 3.12), (4.55 ± 3.12), (12.65 ± 2.30), (19.15 ± 3.58); and in AI: (6.90 ± 4.12), (5.75 ± 4 .12), (5.75 ± 4.12), (11.65 ± 2.05), (18.99 ± 3.2) (P>0.05). Furthermore, and regarding the strains, data revealed that the females did not have any significant effect on the variables studied. On the contrary, the male had a very highly significant effect on the number of mating per fertilizing mating (p = 0.000), while it had no impact on the other parameters (P>0.05). To conclude, the control and the management of reproduction is the key to success in rabbit breeding, thus the combination of rearing condition and genetic effects is the main tool for making rabbit farming successful.
本研究旨在确定兔子的繁殖模式和品系(公兔和母兔)对一些动物技术和繁殖参数的影响。为此,2019 年在塞提夫地区(阿尔及利亚东北部)对 25 只杂交兔的半密集繁殖进行了监测。统计分析显示,繁殖方式对每次受精交配次数的影响非常显著(P= 0.0001),人工授精平均为 1 ± 0(受精率为 80%),而自然繁殖为 1.45 ± 0.51(受精率为 61%)。人工授精兔断奶时的体重高于自然交配兔(分别为 587.25 ± 338.19 g 对 575.0 ± 375.44 g)。自然交配模式下的总出生兔数、活产兔数、断奶兔数、产仔-交配间隔时间和产仔-受精-交配间隔时间分别为(7.4±3.12)、(4.55±3.12)、(4.55±3.12)、(12.65±2.30)、(19.15±3.58);人工授精:(6.90±4.12)、(5.75±4 .12)、(5.75±4.12)、(11.65±2.05)、(18.99±3.2)(P>0.05)。此外,关于菌株,数据显示雌性对所研究的变量没有任何显著影响。相反,雄性对每次受精交配的次数有非常显著的影响(P = 0.000),而对其他参数没有影响(P>0.05)。总之,繁殖的控制和管理是养兔成功的关键,因此,饲养条件和遗传效应的结合是养兔成功的主要工具。
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引用次数: 0
Reproduction mode and Strain effects on Rabbit Breeding Performances 繁殖模式和品系对家兔繁殖性能的影响
Q3 Veterinary Pub Date : 2024-02-13 DOI: 10.46419/vs.55.5.3
Khouloud Dekkiche, Hania Hannani, Achour Menanni, Nassima Ait Issad, D. Khelef, N. Mimoune
This study was carried out to determine the impact of the mode of reproduction and the strain (male and female) of rabbits on some zootechnical and reproduction parameters. For that, monitoring was carried out in 2019 in the region of Setif (North Eastern Algeria) on a semi-intensive breeding of 25 crossbreed rabbits. The statistical analysis showed a very highly significant effect of the mode of reproduction on the number of mating per fertilizing mating (P= 0.0001) with an average of 1 ± 0 in AI (Fertility rate of 80%), while it was of 1.45 ± 0.51 in natural breeding with a fertility rate of 61%. The weight of the rabbits at weaning was higher in AI than in natural mating (587.25 ± 338.19 g vs. 575.0 ± 375.44 g, respectively). The results relating to the number of total born, born alive, weaned rabbits, the farrowing-mating interval and the farrowing-fertilizing mating interval in natural mode were respectively of (7.4 ± 3.12), (4.55 ± 3.12), (4.55 ± 3.12), (12.65 ± 2.30), (19.15 ± 3.58); and in AI: (6.90 ± 4.12), (5.75 ± 4 .12), (5.75 ± 4.12), (11.65 ± 2.05), (18.99 ± 3.2) (P>0.05). Furthermore, and regarding the strains, data revealed that the females did not have any significant effect on the variables studied. On the contrary, the male had a very highly significant effect on the number of mating per fertilizing mating (p = 0.000), while it had no impact on the other parameters (P>0.05). To conclude, the control and the management of reproduction is the key to success in rabbit breeding, thus the combination of rearing condition and genetic effects is the main tool for making rabbit farming successful.
本研究旨在确定兔子的繁殖模式和品系(公兔和母兔)对一些动物技术和繁殖参数的影响。为此,2019 年在塞提夫地区(阿尔及利亚东北部)对 25 只杂交兔的半密集繁殖进行了监测。统计分析显示,繁殖方式对每次受精交配次数的影响非常显著(P= 0.0001),人工授精平均为 1 ± 0(受精率为 80%),而自然繁殖为 1.45 ± 0.51(受精率为 61%)。人工授精兔断奶时的体重高于自然交配兔(分别为 587.25 ± 338.19 g 对 575.0 ± 375.44 g)。自然交配模式下的总出生兔数、活产兔数、断奶兔数、产仔-交配间隔时间和产仔-受精-交配间隔时间分别为(7.4±3.12)、(4.55±3.12)、(4.55±3.12)、(12.65±2.30)、(19.15±3.58);人工授精:(6.90±4.12)、(5.75±4 .12)、(5.75±4.12)、(11.65±2.05)、(18.99±3.2)(P>0.05)。此外,关于菌株,数据显示雌性对所研究的变量没有任何显著影响。相反,雄性对每次受精交配的次数有非常显著的影响(P = 0.000),而对其他参数没有影响(P>0.05)。总之,繁殖的控制和管理是养兔成功的关键,因此,饲养条件和遗传效应的结合是养兔成功的主要工具。
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引用次数: 0
Razotkrivanje stanja: istraživanje prevalencije virusa mačje imunodeficijencije i virusa mačje leukemije među mačkama iz skloništa u Portugalu 了解情况:调查葡萄牙猫科动物中猫科免疫缺陷病毒和猫白血病病毒的流行情况
Q3 Veterinary Pub Date : 2024-02-13 DOI: 10.46419/vs.55.5.1
Paulo Afonso, Luís Cardoso, Hélder Quintas, Ana Cláudia Coelho
Feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) are important pathogens affecting feline hosts worldwide, compromising the health, well-being, quality of life, and lifespan of infected cats. This study explores the prevalence of these viral agents among shelter cats in Portugal, aiming to unravel their complexities and implications. The prevalence of FIV and FeLV was assessed by collecting blood samples (n = 326) from cats during medical routine check-ups at shelter admission and testing them with an immunochromatographic kit. The overall seroprevalence of FIV and FeLV was 15.3% (50/326, 95% confidence interval [CI]: 11.6–19.7). The proportion of cats positive to FIV, FeLV, and both FIV and FeLV was 9.8% (32/326, 95% CI: 6.8-13.6), 2.1% (7/326, 95% CI: 0.9-4.4) and 3.4% (11/326, 95% CI: 1.7-6.0), respectively. The prevalence of FIV and both FeLV and FIV appeared to be higher in the adult population. No statistical association was found between sex, breed, hair, or municipality, and FeLV, FIV and both FeLV and FIV prevalence. This is the first report on retroviral prevalence in shelter cats in Portugal, which provides important information regarding FIV and FeLV among stray cats in Portugal, highlighting the need for surveillance of those viruses, improved compliance with vaccination programmes, and vigilant management strategies to limit and control these viruses in the felinepopulation.
猫免疫缺陷病毒(FIV)和猫白血病病毒(FeLV)是影响全球猫科动物宿主的重要病原体,会损害受感染猫的健康、福祉、生活质量和寿命。本研究探讨了这些病毒病原体在葡萄牙收容所猫咪中的流行情况,旨在揭示其复杂性和影响。在收容所收容的猫咪进行常规体检时,收集它们的血液样本(n = 326),然后用免疫层析试剂盒进行检测,从而评估 FIV 和 FeLV 的流行率。FIV和FeLV的总体血清阳性率为15.3%(50/326,95%置信区间[CI]:11.6-19.7)。FIV、FeLV以及FIV和FeLV均呈阳性的猫咪比例分别为9.8%(32/326,95% CI:6.8-13.6)、2.1%(7/326,95% CI:0.9-4.4)和3.4%(11/326,95% CI:1.7-6.0)。FIV以及FeLV和FIV在成年人群中的流行率似乎较高。性别、品种、毛发或城市与 FeLV、FIV 以及 FeLV 和 FIV 的流行率之间没有统计学关联。这是第一份关于葡萄牙收容猫中逆转录病毒流行情况的报告,它提供了有关葡萄牙流浪猫中 FIV 和 FeLV 的重要信息,强调了对这些病毒进行监测、提高对疫苗接种计划的依从性以及采取警惕性管理策略以限制和控制猫群中这些病毒的必要性。
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引用次数: 0
The influence of sunflower oil in meals on the blood mineral profile of dairy cows 膳食中的葵花籽油对奶牛血液中矿物质含量的影响
Q3 Veterinary Pub Date : 2024-02-13 DOI: 10.46419/vs.55.5.7
E. Šaljić, A. Hrković-Porobija, S. Šerić-Haračić, F. Tandir, Maja Varatanović, A. Ćutuk, B. Čengić
Minerals are essential substances with an important function in livestock, and their disbalance negatively affects the health and production, especially of ruminants. The addition of fat to the rations of dairy cows impacts the overall and mineral metabolism, and thus on health and production. The aim of the research was to examine the correlative relationships between balanced production meals and the mineral status of lactating cows with different ration compositions with or without the presence of vegetable oil. The research was conducted on 30 dairy cows of the Holstein breed at the Butmir farm, Sarajevo, Bosnia and Herzegovina. Three groups of 10 animals were formed based on productivity (A, 13-15 kg of milk/day, B, 19-21 kg of milk/day and C, 25-27 kg of milk/day). Samples of feed, blood plasma and milk of animals were collected on three occasions a 3-week intervals. According to the feed analysis results, the rations were standardized for each group, given for three weeks without oil,and then for three weeks with the addition of sunflower oil in the amount of 2.5% of the ration dry matter. After determining the content of basic nutrients, dry matter and ash in the rations, the concentrations of Ca, Mg, Na and K were determined by atomic absorption spectrophotometry,while the colorimetric method was used to analyse P according to Woy and Eggertz-Finkener. Analysis of blood mineral parameters was performed spectrophotometrically. The content of fat, proteins, lactose, and non-fat dry matter in milk samples was determined by infrared spectrometry using an automatic analyser. Based on the results, we observed a similarity in changes of values and the statistical significance of the differences in plasma Ca, P and Mg between the study groups and at sampling intervals. Milk production was positively correlated with the concentration of Mg and Ca with addition of the sunflower oil to the meal, while no correlation coefficient was established for P in any of the samplings. Balanced meals with and without the addition of oil did not significantly affect the content of Ca, P or Mg in the blood plasma of the tested animals, nor were significant differences found between the groups. However, by determining the correlations between milk parameters and blood biochemical parameters, a significant positive correlation was established between the amount of milk and Ca and Mg levels with a diet supplemented by oil. Given that the addition of sunflower oil in the amount of 2.5% of dry matter of the balanced rations for cows had no negative effects on the mineral profile and the parameters of the tested animals, we conclude that the introduction of this oil could be useful both from the economic point and improving nutritional composition of milk as an animal food present in the daily human diet.
矿物质是牲畜体内的必需物质,具有重要功能,其失衡会对牲畜的健康和生产造成负面影响,尤其是反刍动物。在奶牛日粮中添加脂肪会影响整体和矿物质代谢,从而影响健康和生产。这项研究的目的是探讨在添加或不添加植物油的不同日粮成分下,泌乳奶牛的平衡生产膳食与矿物质状况之间的相关关系。研究对象是波斯尼亚和黑塞哥维那萨拉热窝布特米尔农场的 30 头荷斯坦奶牛。根据生产率将 10 头奶牛分成三组(A 组,每天产奶 13-15 公斤;B 组,每天产奶 19-21 公斤;C 组,每天产奶 25-27 公斤)。每隔 3 周收集三次动物饲料、血浆和牛奶样本。根据饲料分析结果,对各组的日粮进行了标准化,在不添加葵花籽油的情况下饲喂三周,然后添加日粮干物质 2.5% 的葵花籽油饲喂三周。在确定了日粮中基本营养成分、干物质和灰分的含量后,用原子吸收分光光度法测定了 Ca、Mg、Na 和 K 的浓度,并根据 Woy 和 Eggertz-Finkener 的方法用比色法分析了 P 的含量。血液矿物质参数分析采用分光光度法。牛奶样本中的脂肪、蛋白质、乳糖和非脂干物质的含量是通过红外光谱自动分析仪测定的。根据结果,我们观察到各研究组之间和各采样间隔之间血浆中钙、磷和镁的数值变化相似,差异具有统计学意义。在膳食中添加葵花籽油后,牛奶产量与镁和钙的浓度呈正相关,而在任何采样中,钙和磷都没有相关系数。添加葵花籽油和不添加葵花籽油的平衡膳食对受试动物血浆中钙、磷或镁的含量没有显著影响,组间也没有发现明显差异。然而,通过测定牛奶参数与血液生化参数之间的相关性,发现添加油脂的日粮中牛奶量与钙、镁含量之间存在明显的正相关。鉴于在奶牛平衡日粮中添加干物质 2.5% 的葵花籽油对矿物质概况和受测动物的参数没有负面影响,我们得出结论,作为人类日常饮食中的一种动物食品,添加这种油从经济角度和改善牛奶的营养成分角度来看都是有益的。
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引用次数: 0
Testing the potential antifungal activity of Origanum vulgare against Aspergillus fumigatus, Aspergillus Niger and Talaromyces marneffei isolated from pets 测试牛至对从宠物身上分离出的烟曲霉、尼日尔曲霉和马内菲他拉菌的潜在抗真菌活性
Q3 Veterinary Pub Date : 2024-02-13 DOI: 10.46419/vs.55.5.6
João Filipe Rama, Ana Cláudia Coelho, Fernanda Maria Leal
Origanum vulgare (oregano) is an aromatic herb commonly used in the Mediterranean, widely known for its medicinal properties. In this experiment, we tested its antifungal properties against two species of Aspergillus (A. niger and A. fumigatus) and the emerging Talaromyces marneffei. We tested the plant’s antifungal activity in a range of concentrations (5, 10, 20 and 30 mg/mL) in which the plant was dissolved and mixed with growth medium (PDA) and then inoculated with the fungi. The percentage of inhibition was measured over 7 days with data being collected on the 3rd, 5th and 7th days. Fungal activity inhibition ranging from 30 to 100% was achieved against Aspergillus niger and Aspergillus fumigatus and from 50 to 100% against Talaromyces marneffei. This multi-disciplinary study brought the use of plants into the veterinary sciences by using samples isolated from pets, with the aim of researching potential alternatives to traditional antifungal treatments and the ultimate goal ofincreasing animal wellbeing.
牛至(Origanum vulgare)是地中海地区常用的芳香草本植物,因其药用特性而广为人知。在本实验中,我们测试了牛至对两种曲霉(黑曲霉和烟曲霉)以及新出现的马内菲酵母菌(Talaromyces marneffei)的抗真菌特性。我们测试了该植物在不同浓度(5、10、20 和 30 mg/mL)下的抗真菌活性,将该植物溶解并与生长培养基(PDA)混合,然后接种真菌。在第 3、5 和 7 天收集数据,在 7 天内测量抑制率。对黑曲霉和烟曲霉的抑制率从 30%到 100%不等,对马拉色菌的抑制率从 50%到 100%不等。这项多学科研究通过使用从宠物身上分离出的样本,将植物的使用带入了兽医学领域,目的是研究传统抗真菌治疗的潜在替代品,最终目标是提高动物的健康水平。
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引用次数: 0
Genetic characterisation of the domestic cat population (Felis catus) in Valledupar – Cesar using coat markers 利用皮毛标记分析巴耶杜帕尔-塞萨尔地区家猫(Felis catus)种群的遗传特征
Q3 Veterinary Pub Date : 2024-02-13 DOI: 10.46419/vs.55.5.5
Enrique Pardo-Pérez, Teodora Cavadía-Martínez, Luis Causil-Vargas
The domestic cat (Felis catus) is a small carnivorous mammal belonging to the feline family. It is characterised by significant variability in terms of breeds, with females commonly smaller than males. Cats are one of the most popular pet species worldwide, though there is growing evidence that their large populations are having negative effects on the environment. Cats exhibit noticeable polymorphisms, particularly related to the colour, pattern, and texture of their coat, which can be easily recognised by visual inspection. This makes data collection a straightforward procedure. Cats are an ideal species for population studies because they are cosmopolitan animals and form a panmictic population. Genetic population analyses of cats are essential for understanding the history of their evolution and for developing phylogenetic hypotheses about allele relationships. However, despite the importance of these studies, global information availability is limited and, in some areas, completely absent. The aim of this study wasto determine the level of genetic diversity and structure in domestic cat populations using coat markers in Valledupar, Cesar. Random sampling was conducted between February and September 2022, and 365 adult animals were phenotypically identified in twelve neighbourhoods of the locality. The genes studied included Orange, Agouti, Dilution, Tabby, Long Hair, Siamese, Manx, Spotting White, and Dominant White. The Non-agoutimarker showed the highest frequency (0.740), while the Manx gene exhibited the lowest value. Most of the genetic diversity was found within populations and was limited between populations. Additionally, high gene flow, an excess of heterozygotes, and reduced genetic differentiation between populations were observed. The Spotting White locus showed a departure from Hardy-Weinberg equilibrium. Genetic distance between populations and the obtained dendrogram revealed low significant values, indicating that the populations as a whole are closely related.
家猫(Felis catus)是猫科的一种小型食肉哺乳动物。其特点是品种繁多,雌猫通常比雄猫小。猫是全球最受欢迎的宠物物种之一,但越来越多的证据表明,猫的大量繁殖对环境造成了负面影响。猫表现出明显的多态性,尤其是在毛色、花纹和质地方面,这很容易通过肉眼识别。这使得数据收集变得简单易行。猫是一种理想的种群研究物种,因为它们是世界性动物,可形成泛种群。猫的种群遗传分析对于了解猫的进化史和提出等位基因关系的系统发育假设至关重要。然而,尽管这些研究非常重要,但全球可用的信息却很有限,在某些地区甚至完全没有。本研究旨在利用毛皮标记确定塞萨尔巴耶杜帕尔地区家猫种群的遗传多样性和结构水平。研究在 2022 年 2 月至 9 月期间进行了随机取样,在当地的 12 个社区对 365 只成年猫进行了表型鉴定。研究的基因包括橙色、阿古提、稀释、虎斑、长毛、暹罗、曼克斯、斑点白和显性白。非阿古提基因的频率最高(0.740),而曼克斯基因的频率最低。大部分遗传多样性出现在种群内部,而在种群之间则很有限。此外,还观察到种群间基因流动频繁、杂合子过多和遗传分化减少。斑白基因座偏离了哈代-温伯格平衡。种群间的遗传距离和所获得的树枝图显示出较低的显著值,表明种群作为一个整体具有密切的亲缘关系。
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Veterinarska stanica
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