Lyda C. Caballero Méndez, Luisa María González Londoño, Julio Cesar Gómez Ruíz, María José Escobar Herrera, Margarita María Mazo, Luz Natalia Franco-Montoya
Leptospirosis is considered a zoonotic disease with a substantial impact on animal and human health. It is distributed in tropical and subtropical climates, which improves the survival of these bacteria for a long time, affecting domestic and wild animals that act as a reservoir. Canine leptospirosis has obtained great clinical relevance, due to the susceptibility of this species to infection and the frequent exposure to leptospirosis from the environment. Canines from the street and animal shelters constitute a high-risk population due to the proximity to sources of infection. This paper describes a cross-sectional study that involves sampling dogs from shelters located in the departments of Risaralda, Valle del Cauca, and Caldas, known as the Coffee Region, located in the centre-west of Colombia. Blood samples were taken from 140 canines and analysed in the laboratory of the Veterinary Medicine and Animal Science, Technological University of Pereira. DNA was extracted, and the LipL32 gene was amplified by conventional PCR. A 15% prevalence for Leptospira sp. was found in dogs from the Coffee region. No correlation was found between the variables such as sex, age, origin, and socioeconomic status. However, a tendency for infection was observed with several cases of diagnosis in female dogs older than six years in low strata. This study constitutes the first report of canine leptospirosis in this region of Colombia, which will allow the design of strategies aiming to mitigate the disease in this region.
{"title":"Molekularno određivanje Leptospira spp. u uličnim psima i psima iz skloništa u kolumbijskoj Regiji kave","authors":"Lyda C. Caballero Méndez, Luisa María González Londoño, Julio Cesar Gómez Ruíz, María José Escobar Herrera, Margarita María Mazo, Luz Natalia Franco-Montoya","doi":"10.46419/vs.55.5.2","DOIUrl":"https://doi.org/10.46419/vs.55.5.2","url":null,"abstract":"Leptospirosis is considered a zoonotic disease with a substantial impact on animal and human health. It is distributed in tropical and subtropical climates, which improves the survival of these bacteria for a long time, affecting domestic and wild animals that act as a reservoir. Canine leptospirosis has obtained great clinical relevance, due to the susceptibility of this species to infection and the frequent exposure to leptospirosis from the environment. Canines from the street and animal shelters constitute a high-risk population due to the proximity to sources of infection. This paper describes a cross-sectional study that involves sampling dogs from shelters located in the departments of Risaralda, Valle del Cauca, and Caldas, known as the Coffee Region, located in the centre-west of Colombia. Blood samples were taken from 140 canines and analysed in the laboratory of the Veterinary Medicine and Animal Science, Technological University of Pereira. DNA was extracted, and the LipL32 gene was amplified by conventional PCR. A 15% prevalence for Leptospira sp. was found in dogs from the Coffee region. No correlation was found between the variables such as sex, age, origin, and socioeconomic status. However, a tendency for infection was observed with several cases of diagnosis in female dogs older than six years in low strata. This study constitutes the first report of canine leptospirosis in this region of Colombia, which will allow the design of strategies aiming to mitigate the disease in this region.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139779939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lyda C. Caballero Méndez, Luisa María González Londoño, Julio Cesar Gómez Ruíz, María José Escobar Herrera, Margarita María Mazo, Luz Natalia Franco-Montoya
Leptospirosis is considered a zoonotic disease with a substantial impact on animal and human health. It is distributed in tropical and subtropical climates, which improves the survival of these bacteria for a long time, affecting domestic and wild animals that act as a reservoir. Canine leptospirosis has obtained great clinical relevance, due to the susceptibility of this species to infection and the frequent exposure to leptospirosis from the environment. Canines from the street and animal shelters constitute a high-risk population due to the proximity to sources of infection. This paper describes a cross-sectional study that involves sampling dogs from shelters located in the departments of Risaralda, Valle del Cauca, and Caldas, known as the Coffee Region, located in the centre-west of Colombia. Blood samples were taken from 140 canines and analysed in the laboratory of the Veterinary Medicine and Animal Science, Technological University of Pereira. DNA was extracted, and the LipL32 gene was amplified by conventional PCR. A 15% prevalence for Leptospira sp. was found in dogs from the Coffee region. No correlation was found between the variables such as sex, age, origin, and socioeconomic status. However, a tendency for infection was observed with several cases of diagnosis in female dogs older than six years in low strata. This study constitutes the first report of canine leptospirosis in this region of Colombia, which will allow the design of strategies aiming to mitigate the disease in this region.
{"title":"Molekularno određivanje Leptospira spp. u uličnim psima i psima iz skloništa u kolumbijskoj Regiji kave","authors":"Lyda C. Caballero Méndez, Luisa María González Londoño, Julio Cesar Gómez Ruíz, María José Escobar Herrera, Margarita María Mazo, Luz Natalia Franco-Montoya","doi":"10.46419/vs.55.5.2","DOIUrl":"https://doi.org/10.46419/vs.55.5.2","url":null,"abstract":"Leptospirosis is considered a zoonotic disease with a substantial impact on animal and human health. It is distributed in tropical and subtropical climates, which improves the survival of these bacteria for a long time, affecting domestic and wild animals that act as a reservoir. Canine leptospirosis has obtained great clinical relevance, due to the susceptibility of this species to infection and the frequent exposure to leptospirosis from the environment. Canines from the street and animal shelters constitute a high-risk population due to the proximity to sources of infection. This paper describes a cross-sectional study that involves sampling dogs from shelters located in the departments of Risaralda, Valle del Cauca, and Caldas, known as the Coffee Region, located in the centre-west of Colombia. Blood samples were taken from 140 canines and analysed in the laboratory of the Veterinary Medicine and Animal Science, Technological University of Pereira. DNA was extracted, and the LipL32 gene was amplified by conventional PCR. A 15% prevalence for Leptospira sp. was found in dogs from the Coffee region. No correlation was found between the variables such as sex, age, origin, and socioeconomic status. However, a tendency for infection was observed with several cases of diagnosis in female dogs older than six years in low strata. This study constitutes the first report of canine leptospirosis in this region of Colombia, which will allow the design of strategies aiming to mitigate the disease in this region.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139839708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In veterinary medicine, liver biopsy is a standard diagnostic procedure for studying liver disease in animal species, including cows. Some common indications for liver biopsy in cattle include clinical signs of liver disease (e.g., anorexia, jaundice, lethargy), unexplained weight loss, and monitoring of health and nutritional status. This technique comes with certain risks, such as haemorrhage, infections, and lesions of other organs, so it shouldonly be carried out by a trained and experienced veterinarian. The Clinical Skills Laboratory is a structured teaching laboratory that provides hands-on experience to veterinary medicine students in a safe, controlled environment. The laboratory focuses on developing practical competencies in various veterinary procedures. This paper presents the development of a model for performing liver biopsy in cattle and discusses the importance of the skills lab in veterinary medicine learning. This model enables students to learn how to perform a liver biopsy in cattle and the basic rules of sampling and fixation of the specimen for histopathological examination. It is accompanied by detailed work instructions and illustrations to assist the student in performing the procedure. This kind of laboratory is indispensable in veterinary medical training, as it helps students strengthen their skills and confidence, preparing them for a successful career in veterinary medicine. In addition, it allows for the execution of techniques that would not be practised on a live animal during classes.
{"title":"Development of a bovine liver biopsy training model","authors":"Filipe Silva, Isabel Pires","doi":"10.46419/vs.55.5.9","DOIUrl":"https://doi.org/10.46419/vs.55.5.9","url":null,"abstract":"In veterinary medicine, liver biopsy is a standard diagnostic procedure for studying liver disease in animal species, including cows. Some common indications for liver biopsy in cattle include clinical signs of liver disease (e.g., anorexia, jaundice, lethargy), unexplained weight loss, and monitoring of health and nutritional status. This technique comes with certain risks, such as haemorrhage, infections, and lesions of other organs, so it shouldonly be carried out by a trained and experienced veterinarian. The Clinical Skills Laboratory is a structured teaching laboratory that provides hands-on experience to veterinary medicine students in a safe, controlled environment. The laboratory focuses on developing practical competencies in various veterinary procedures. This paper presents the development of a model for performing liver biopsy in cattle and discusses the importance of the skills lab in veterinary medicine learning. This model enables students to learn how to perform a liver biopsy in cattle and the basic rules of sampling and fixation of the specimen for histopathological examination. It is accompanied by detailed work instructions and illustrations to assist the student in performing the procedure. This kind of laboratory is indispensable in veterinary medical training, as it helps students strengthen their skills and confidence, preparing them for a successful career in veterinary medicine. In addition, it allows for the execution of techniques that would not be practised on a live animal during classes.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139780169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Petar Dodovski, Talija Hristovska, Natasa Pejcinovska, P. Dameski, Nikola Karabolovski, Mimi Ristevski
The Ovcepolian type of Pramenka sheep is most prevalent in Pelagonia region. It is considered a valuable source of genetic diversity, characterised with a number of beneficial traits. Haematology and biochemistry measurements is important for health assessment of animals. The aim of this study was to determine the reference ranges for selected haematology and biochemistry parameters in the Pelagonian Pramenka sheep breed. The study was carried out on 55 clinically healthy, non-pregnant sheep, 1 to 3 years old, during spring season, from three different locations in the Pelagonia region, North Macedonia. Blood samples were collected from the jugular vein into vacuum tubes with an anticoagulant for haematological analysis and vacuum tubes free of anticoagulant for biochemical analysis. Reference values may be more adequate as it is later, for biochemistry parameters. Reference values for hematology parameters were: leukocytes, lymphocytes, midsize cells, cells correlating to monocytes, eosinophils, basophils, blasts and other precursor white cells, granulocytes, haemoglobin, haematocrit, erythrocytes, erythrocyte indices (average volume per litre, average haemoglobin in erythrocytes, average haemoglobin concentration per litre, and platelets). Reference values for biochemistry parameters were: total proteins, albumins, total bilirubin, total cholesterol, creatinine, glucose, HDL-cholesterol LDL-cholesterol, triglycerides, urea, aspartate aminotransferase, alanine amino transferase, alkaline phosphatase, glutamyl transferase, lactate dehydrogenase, and the concentrations of calcium, magnesium and inorganic phosphate. Most obtained values were comparable to other strains of Pramenka sheep. There was a clear deviation of certain reference values compared to the literature. Further analysis should be conducted to determine the reference values in rams and lambs.
{"title":"Referentne vrijednosti za odabrane hematološke i biokemijske parametre u pelagonijske pramenka ovce","authors":"Petar Dodovski, Talija Hristovska, Natasa Pejcinovska, P. Dameski, Nikola Karabolovski, Mimi Ristevski","doi":"10.46419/vs.55.5.4","DOIUrl":"https://doi.org/10.46419/vs.55.5.4","url":null,"abstract":"The Ovcepolian type of Pramenka sheep is most prevalent in Pelagonia region. It is considered a valuable source of genetic diversity, characterised with a number of beneficial traits. Haematology and biochemistry measurements is important for health assessment of animals. The aim of this study was to determine the reference ranges for selected haematology and biochemistry parameters in the Pelagonian Pramenka sheep breed. The study was carried out on 55 clinically healthy, non-pregnant sheep, 1 to 3 years old, during spring season, from three different locations in the Pelagonia region, North Macedonia. Blood samples were collected from the jugular vein into vacuum tubes with an anticoagulant for haematological analysis and vacuum tubes free of anticoagulant for biochemical analysis. Reference values may be more adequate as it is later, for biochemistry parameters. Reference values for hematology parameters were: leukocytes, lymphocytes, midsize cells, cells correlating to monocytes, eosinophils, basophils, blasts and other precursor white cells, granulocytes, haemoglobin, haematocrit, erythrocytes, erythrocyte indices (average volume per litre, average haemoglobin in erythrocytes, average haemoglobin concentration per litre, and platelets). Reference values for biochemistry parameters were: total proteins, albumins, total bilirubin, total cholesterol, creatinine, glucose, HDL-cholesterol LDL-cholesterol, triglycerides, urea, aspartate aminotransferase, alanine amino transferase, alkaline phosphatase, glutamyl transferase, lactate dehydrogenase, and the concentrations of calcium, magnesium and inorganic phosphate. Most obtained values were comparable to other strains of Pramenka sheep. There was a clear deviation of certain reference values compared to the literature. Further analysis should be conducted to determine the reference values in rams and lambs.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139781113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Šaljić, A. Hrković-Porobija, S. Šerić-Haračić, F. Tandir, Maja Varatanović, A. Ćutuk, B. Čengić
Minerals are essential substances with an important function in livestock, and their disbalance negatively affects the health and production, especially of ruminants. The addition of fat to the rations of dairy cows impacts the overall and mineral metabolism, and thus on health and production. The aim of the research was to examine the correlative relationships between balanced production meals and the mineral status of lactating cows with different ration compositions with or without the presence of vegetable oil. The research was conducted on 30 dairy cows of the Holstein breed at the Butmir farm, Sarajevo, Bosnia and Herzegovina. Three groups of 10 animals were formed based on productivity (A, 13-15 kg of milk/day, B, 19-21 kg of milk/day and C, 25-27 kg of milk/day). Samples of feed, blood plasma and milk of animals were collected on three occasions a 3-week intervals. According to the feed analysis results, the rations were standardized for each group, given for three weeks without oil,and then for three weeks with the addition of sunflower oil in the amount of 2.5% of the ration dry matter. After determining the content of basic nutrients, dry matter and ash in the rations, the concentrations of Ca, Mg, Na and K were determined by atomic absorption spectrophotometry,while the colorimetric method was used to analyse P according to Woy and Eggertz-Finkener. Analysis of blood mineral parameters was performed spectrophotometrically. The content of fat, proteins, lactose, and non-fat dry matter in milk samples was determined by infrared spectrometry using an automatic analyser. Based on the results, we observed a similarity in changes of values and the statistical significance of the differences in plasma Ca, P and Mg between the study groups and at sampling intervals. Milk production was positively correlated with the concentration of Mg and Ca with addition of the sunflower oil to the meal, while no correlation coefficient was established for P in any of the samplings. Balanced meals with and without the addition of oil did not significantly affect the content of Ca, P or Mg in the blood plasma of the tested animals, nor were significant differences found between the groups. However, by determining the correlations between milk parameters and blood biochemical parameters, a significant positive correlation was established between the amount of milk and Ca and Mg levels with a diet supplemented by oil. Given that the addition of sunflower oil in the amount of 2.5% of dry matter of the balanced rations for cows had no negative effects on the mineral profile and the parameters of the tested animals, we conclude that the introduction of this oil could be useful both from the economic point and improving nutritional composition of milk as an animal food present in the daily human diet.
{"title":"The influence of sunflower oil in meals on the blood mineral profile of dairy cows","authors":"E. Šaljić, A. Hrković-Porobija, S. Šerić-Haračić, F. Tandir, Maja Varatanović, A. Ćutuk, B. Čengić","doi":"10.46419/vs.55.5.7","DOIUrl":"https://doi.org/10.46419/vs.55.5.7","url":null,"abstract":"Minerals are essential substances with an important function in livestock, and their disbalance negatively affects the health and production, especially of ruminants. The addition of fat to the rations of dairy cows impacts the overall and mineral metabolism, and thus on health and production. The aim of the research was to examine the correlative relationships between balanced production meals and the mineral status of lactating cows with different ration compositions with or without the presence of vegetable oil. The research was conducted on 30 dairy cows of the Holstein breed at the Butmir farm, Sarajevo, Bosnia and Herzegovina. Three groups of 10 animals were formed based on productivity (A, 13-15 kg of milk/day, B, 19-21 kg of milk/day and C, 25-27 kg of milk/day). Samples of feed, blood plasma and milk of animals were collected on three occasions a 3-week intervals. According to the feed analysis results, the rations were standardized for each group, given for three weeks without oil,and then for three weeks with the addition of sunflower oil in the amount of 2.5% of the ration dry matter. After determining the content of basic nutrients, dry matter and ash in the rations, the concentrations of Ca, Mg, Na and K were determined by atomic absorption spectrophotometry,while the colorimetric method was used to analyse P according to Woy and Eggertz-Finkener. Analysis of blood mineral parameters was performed spectrophotometrically. The content of fat, proteins, lactose, and non-fat dry matter in milk samples was determined by infrared spectrometry using an automatic analyser. Based on the results, we observed a similarity in changes of values and the statistical significance of the differences in plasma Ca, P and Mg between the study groups and at sampling intervals. Milk production was positively correlated with the concentration of Mg and Ca with addition of the sunflower oil to the meal, while no correlation coefficient was established for P in any of the samplings. Balanced meals with and without the addition of oil did not significantly affect the content of Ca, P or Mg in the blood plasma of the tested animals, nor were significant differences found between the groups. However, by determining the correlations between milk parameters and blood biochemical parameters, a significant positive correlation was established between the amount of milk and Ca and Mg levels with a diet supplemented by oil. Given that the addition of sunflower oil in the amount of 2.5% of dry matter of the balanced rations for cows had no negative effects on the mineral profile and the parameters of the tested animals, we conclude that the introduction of this oil could be useful both from the economic point and improving nutritional composition of milk as an animal food present in the daily human diet.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139839452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In veterinary medicine, liver biopsy is a standard diagnostic procedure for studying liver disease in animal species, including cows. Some common indications for liver biopsy in cattle include clinical signs of liver disease (e.g., anorexia, jaundice, lethargy), unexplained weight loss, and monitoring of health and nutritional status. This technique comes with certain risks, such as haemorrhage, infections, and lesions of other organs, so it shouldonly be carried out by a trained and experienced veterinarian. The Clinical Skills Laboratory is a structured teaching laboratory that provides hands-on experience to veterinary medicine students in a safe, controlled environment. The laboratory focuses on developing practical competencies in various veterinary procedures. This paper presents the development of a model for performing liver biopsy in cattle and discusses the importance of the skills lab in veterinary medicine learning. This model enables students to learn how to perform a liver biopsy in cattle and the basic rules of sampling and fixation of the specimen for histopathological examination. It is accompanied by detailed work instructions and illustrations to assist the student in performing the procedure. This kind of laboratory is indispensable in veterinary medical training, as it helps students strengthen their skills and confidence, preparing them for a successful career in veterinary medicine. In addition, it allows for the execution of techniques that would not be practised on a live animal during classes.
{"title":"Development of a bovine liver biopsy training model","authors":"Filipe Silva, Isabel Pires","doi":"10.46419/vs.55.5.9","DOIUrl":"https://doi.org/10.46419/vs.55.5.9","url":null,"abstract":"In veterinary medicine, liver biopsy is a standard diagnostic procedure for studying liver disease in animal species, including cows. Some common indications for liver biopsy in cattle include clinical signs of liver disease (e.g., anorexia, jaundice, lethargy), unexplained weight loss, and monitoring of health and nutritional status. This technique comes with certain risks, such as haemorrhage, infections, and lesions of other organs, so it shouldonly be carried out by a trained and experienced veterinarian. The Clinical Skills Laboratory is a structured teaching laboratory that provides hands-on experience to veterinary medicine students in a safe, controlled environment. The laboratory focuses on developing practical competencies in various veterinary procedures. This paper presents the development of a model for performing liver biopsy in cattle and discusses the importance of the skills lab in veterinary medicine learning. This model enables students to learn how to perform a liver biopsy in cattle and the basic rules of sampling and fixation of the specimen for histopathological examination. It is accompanied by detailed work instructions and illustrations to assist the student in performing the procedure. This kind of laboratory is indispensable in veterinary medical training, as it helps students strengthen their skills and confidence, preparing them for a successful career in veterinary medicine. In addition, it allows for the execution of techniques that would not be practised on a live animal during classes.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139839992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Petar Dodovski, Talija Hristovska, Natasa Pejcinovska, P. Dameski, Nikola Karabolovski, Mimi Ristevski
The Ovcepolian type of Pramenka sheep is most prevalent in Pelagonia region. It is considered a valuable source of genetic diversity, characterised with a number of beneficial traits. Haematology and biochemistry measurements is important for health assessment of animals. The aim of this study was to determine the reference ranges for selected haematology and biochemistry parameters in the Pelagonian Pramenka sheep breed. The study was carried out on 55 clinically healthy, non-pregnant sheep, 1 to 3 years old, during spring season, from three different locations in the Pelagonia region, North Macedonia. Blood samples were collected from the jugular vein into vacuum tubes with an anticoagulant for haematological analysis and vacuum tubes free of anticoagulant for biochemical analysis. Reference values may be more adequate as it is later, for biochemistry parameters. Reference values for hematology parameters were: leukocytes, lymphocytes, midsize cells, cells correlating to monocytes, eosinophils, basophils, blasts and other precursor white cells, granulocytes, haemoglobin, haematocrit, erythrocytes, erythrocyte indices (average volume per litre, average haemoglobin in erythrocytes, average haemoglobin concentration per litre, and platelets). Reference values for biochemistry parameters were: total proteins, albumins, total bilirubin, total cholesterol, creatinine, glucose, HDL-cholesterol LDL-cholesterol, triglycerides, urea, aspartate aminotransferase, alanine amino transferase, alkaline phosphatase, glutamyl transferase, lactate dehydrogenase, and the concentrations of calcium, magnesium and inorganic phosphate. Most obtained values were comparable to other strains of Pramenka sheep. There was a clear deviation of certain reference values compared to the literature. Further analysis should be conducted to determine the reference values in rams and lambs.
{"title":"Referentne vrijednosti za odabrane hematološke i biokemijske parametre u pelagonijske pramenka ovce","authors":"Petar Dodovski, Talija Hristovska, Natasa Pejcinovska, P. Dameski, Nikola Karabolovski, Mimi Ristevski","doi":"10.46419/vs.55.5.4","DOIUrl":"https://doi.org/10.46419/vs.55.5.4","url":null,"abstract":"The Ovcepolian type of Pramenka sheep is most prevalent in Pelagonia region. It is considered a valuable source of genetic diversity, characterised with a number of beneficial traits. Haematology and biochemistry measurements is important for health assessment of animals. The aim of this study was to determine the reference ranges for selected haematology and biochemistry parameters in the Pelagonian Pramenka sheep breed. The study was carried out on 55 clinically healthy, non-pregnant sheep, 1 to 3 years old, during spring season, from three different locations in the Pelagonia region, North Macedonia. Blood samples were collected from the jugular vein into vacuum tubes with an anticoagulant for haematological analysis and vacuum tubes free of anticoagulant for biochemical analysis. Reference values may be more adequate as it is later, for biochemistry parameters. Reference values for hematology parameters were: leukocytes, lymphocytes, midsize cells, cells correlating to monocytes, eosinophils, basophils, blasts and other precursor white cells, granulocytes, haemoglobin, haematocrit, erythrocytes, erythrocyte indices (average volume per litre, average haemoglobin in erythrocytes, average haemoglobin concentration per litre, and platelets). Reference values for biochemistry parameters were: total proteins, albumins, total bilirubin, total cholesterol, creatinine, glucose, HDL-cholesterol LDL-cholesterol, triglycerides, urea, aspartate aminotransferase, alanine amino transferase, alkaline phosphatase, glutamyl transferase, lactate dehydrogenase, and the concentrations of calcium, magnesium and inorganic phosphate. Most obtained values were comparable to other strains of Pramenka sheep. There was a clear deviation of certain reference values compared to the literature. Further analysis should be conducted to determine the reference values in rams and lambs.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139840949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Enrique Pardo-Pérez, Teodora Cavadía-Martínez, Luis Causil-Vargas
The domestic cat (Felis catus) is a small carnivorous mammal belonging to the feline family. It is characterised by significant variability in terms of breeds, with females commonly smaller than males. Cats are one of the most popular pet species worldwide, though there is growing evidence that their large populations are having negative effects on the environment. Cats exhibit noticeable polymorphisms, particularly related to the colour, pattern, and texture of their coat, which can be easily recognised by visual inspection. This makes data collection a straightforward procedure. Cats are an ideal species for population studies because they are cosmopolitan animals and form a panmictic population. Genetic population analyses of cats are essential for understanding the history of their evolution and for developing phylogenetic hypotheses about allele relationships. However, despite the importance of these studies, global information availability is limited and, in some areas, completely absent. The aim of this study wasto determine the level of genetic diversity and structure in domestic cat populations using coat markers in Valledupar, Cesar. Random sampling was conducted between February and September 2022, and 365 adult animals were phenotypically identified in twelve neighbourhoods of the locality. The genes studied included Orange, Agouti, Dilution, Tabby, Long Hair, Siamese, Manx, Spotting White, and Dominant White. The Non-agoutimarker showed the highest frequency (0.740), while the Manx gene exhibited the lowest value. Most of the genetic diversity was found within populations and was limited between populations. Additionally, high gene flow, an excess of heterozygotes, and reduced genetic differentiation between populations were observed. The Spotting White locus showed a departure from Hardy-Weinberg equilibrium. Genetic distance between populations and the obtained dendrogram revealed low significant values, indicating that the populations as a whole are closely related.
{"title":"Genetic characterisation of the domestic cat population (Felis catus) in Valledupar – Cesar using coat markers","authors":"Enrique Pardo-Pérez, Teodora Cavadía-Martínez, Luis Causil-Vargas","doi":"10.46419/vs.55.5.5","DOIUrl":"https://doi.org/10.46419/vs.55.5.5","url":null,"abstract":"The domestic cat (Felis catus) is a small carnivorous mammal belonging to the feline family. It is characterised by significant variability in terms of breeds, with females commonly smaller than males. Cats are one of the most popular pet species worldwide, though there is growing evidence that their large populations are having negative effects on the environment. Cats exhibit noticeable polymorphisms, particularly related to the colour, pattern, and texture of their coat, which can be easily recognised by visual inspection. This makes data collection a straightforward procedure. Cats are an ideal species for population studies because they are cosmopolitan animals and form a panmictic population. Genetic population analyses of cats are essential for understanding the history of their evolution and for developing phylogenetic hypotheses about allele relationships. However, despite the importance of these studies, global information availability is limited and, in some areas, completely absent. The aim of this study wasto determine the level of genetic diversity and structure in domestic cat populations using coat markers in Valledupar, Cesar. Random sampling was conducted between February and September 2022, and 365 adult animals were phenotypically identified in twelve neighbourhoods of the locality. The genes studied included Orange, Agouti, Dilution, Tabby, Long Hair, Siamese, Manx, Spotting White, and Dominant White. The Non-agoutimarker showed the highest frequency (0.740), while the Manx gene exhibited the lowest value. Most of the genetic diversity was found within populations and was limited between populations. Additionally, high gene flow, an excess of heterozygotes, and reduced genetic differentiation between populations were observed. The Spotting White locus showed a departure from Hardy-Weinberg equilibrium. Genetic distance between populations and the obtained dendrogram revealed low significant values, indicating that the populations as a whole are closely related.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139841564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, a database was created by monitoring 3000 Ouled Djellal ewes, belonging to ten breeding farms and representing two modes of reproduction management. The data collected make it possible to measure the reproductive performance of females and to determine the influence of non-genetic factors on different parameters. The Body Condition Score (BCS) was calculated to compare performance in relation with breeding conditions. The results showed that fertility and prolificacy accounted for 91.1±0.1% and 116.4±0.2% in the reproduction mode respectively based on one lambing/year with exclusive spring control, and of 53.5±0.2% and 101.2±0.1% respectively in the traditional lambing system spread overthe year (with the ram permanently present in the flock) (P<0.05). The BCS varied according to mating season and feeding system. Indeed, as the BCS at mating increased, fertility, prolificacy and productivity improved. Ewes whose BCS at mating was equal to or greater than 3 obtained the best performance, while those with a lower BCS were less fertile, less prolific, and less productive. The average productivity of Ouled Djellal sheep was 107.0±0.1%, and 54.8±0.3%, respectively, in the first and second mode of reproduction management (P<0.05). This study highlights the main sources of variations and their interactions.
{"title":"Utjecaj sezone parenja i stanja tijela na zootehničke parametre ouled djellal ovaca u polupustinjskim područjima","authors":"Mourad Taherti, Nassima Ait Isad, Nora Mimoune","doi":"10.46419/vs.55.5.10","DOIUrl":"https://doi.org/10.46419/vs.55.5.10","url":null,"abstract":"In this study, a database was created by monitoring 3000 Ouled Djellal ewes, belonging to ten breeding farms and representing two modes of reproduction management. The data collected make it possible to measure the reproductive performance of females and to determine the influence of non-genetic factors on different parameters. The Body Condition Score (BCS) was calculated to compare performance in relation with breeding conditions. The results showed that fertility and prolificacy accounted for 91.1±0.1% and 116.4±0.2% in the reproduction mode respectively based on one lambing/year with exclusive spring control, and of 53.5±0.2% and 101.2±0.1% respectively in the traditional lambing system spread overthe year (with the ram permanently present in the flock) (P<0.05). The BCS varied according to mating season and feeding system. Indeed, as the BCS at mating increased, fertility, prolificacy and productivity improved. Ewes whose BCS at mating was equal to or greater than 3 obtained the best performance, while those with a lower BCS were less fertile, less prolific, and less productive. The average productivity of Ouled Djellal sheep was 107.0±0.1%, and 54.8±0.3%, respectively, in the first and second mode of reproduction management (P<0.05). This study highlights the main sources of variations and their interactions.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139783323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, a database was created by monitoring 3000 Ouled Djellal ewes, belonging to ten breeding farms and representing two modes of reproduction management. The data collected make it possible to measure the reproductive performance of females and to determine the influence of non-genetic factors on different parameters. The Body Condition Score (BCS) was calculated to compare performance in relation with breeding conditions. The results showed that fertility and prolificacy accounted for 91.1±0.1% and 116.4±0.2% in the reproduction mode respectively based on one lambing/year with exclusive spring control, and of 53.5±0.2% and 101.2±0.1% respectively in the traditional lambing system spread overthe year (with the ram permanently present in the flock) (P<0.05). The BCS varied according to mating season and feeding system. Indeed, as the BCS at mating increased, fertility, prolificacy and productivity improved. Ewes whose BCS at mating was equal to or greater than 3 obtained the best performance, while those with a lower BCS were less fertile, less prolific, and less productive. The average productivity of Ouled Djellal sheep was 107.0±0.1%, and 54.8±0.3%, respectively, in the first and second mode of reproduction management (P<0.05). This study highlights the main sources of variations and their interactions.
{"title":"Utjecaj sezone parenja i stanja tijela na zootehničke parametre ouled djellal ovaca u polupustinjskim područjima","authors":"Mourad Taherti, Nassima Ait Isad, Nora Mimoune","doi":"10.46419/vs.55.5.10","DOIUrl":"https://doi.org/10.46419/vs.55.5.10","url":null,"abstract":"In this study, a database was created by monitoring 3000 Ouled Djellal ewes, belonging to ten breeding farms and representing two modes of reproduction management. The data collected make it possible to measure the reproductive performance of females and to determine the influence of non-genetic factors on different parameters. The Body Condition Score (BCS) was calculated to compare performance in relation with breeding conditions. The results showed that fertility and prolificacy accounted for 91.1±0.1% and 116.4±0.2% in the reproduction mode respectively based on one lambing/year with exclusive spring control, and of 53.5±0.2% and 101.2±0.1% respectively in the traditional lambing system spread overthe year (with the ram permanently present in the flock) (P<0.05). The BCS varied according to mating season and feeding system. Indeed, as the BCS at mating increased, fertility, prolificacy and productivity improved. Ewes whose BCS at mating was equal to or greater than 3 obtained the best performance, while those with a lower BCS were less fertile, less prolific, and less productive. The average productivity of Ouled Djellal sheep was 107.0±0.1%, and 54.8±0.3%, respectively, in the first and second mode of reproduction management (P<0.05). This study highlights the main sources of variations and their interactions.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139843311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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