I. Vlahek, Hrvoje Kabalin, S. Faraguna, J. Šavorić, A. Piplica, M. Maurić Maljković, V. Sušić
Reproductive traits are of paramountimportance in an efficient lamb production system. In Croatia, values of reproductive traits of Romanov sheep are below the average expected for this breed, indicating the need for improvement of these traits to exploit the breed’s full reproductive potential. This study was conducted on 260 Romanov ewes located at a commercial Romanov sheep farm in Croatia. All ewes were kept under similar conditions, and reproductive management was based on accelerated lambing with continuous mating. Linear models with fixed effects were used to estimate the influence of year of birth/lambing, season of birth/lambing, ram, parity and litter type on litter size (LS), age at first lambing (AFL) and lambing interval (LI). Average LS was 2.11 ± 0.71, while the year of lambing and parity were the most important factors affecting this trait. The smallest litters were recorded after 1st parity (1.77 ± 0.06), and the largest after 5th parity (2.21 ± 0.08). AFL averaged 388.5 ± 72.4 days. All investigated non-genetic factors had a significant (P<0.05) influence on AFL, with year of birth as most important. The average LI was 241.2 ± 70.8 days. A significant (P<0.05) influence of all factors on LI was observed. Ewes lambing in spring (200.2 ± 7.9 days) or summer (190.5 ±6.6 days) had a significantly (P<0.05) shorter LI than ewes lambing in autumn (227.7 ± 8.3 days) or winter (237.2 ± 6.7 days). The longest LI was observed after the first parity (284.0 ±5.5 days). Average values for reproductive traits in the examined population of Romanov sheep were higher than those reported in the official Croatian database for breeding sheep. Non-genetic factors have a significant influence on the variation of reproductive traits of Romanov sheep, and therefore should be considered when assessing ewes’ reproductive performance.
{"title":"Non-genetic factors affecting litter size, age at first lambing and lambing interval of Romanov sheep in Croatia","authors":"I. Vlahek, Hrvoje Kabalin, S. Faraguna, J. Šavorić, A. Piplica, M. Maurić Maljković, V. Sušić","doi":"10.46419/vs.54.3.6","DOIUrl":"https://doi.org/10.46419/vs.54.3.6","url":null,"abstract":"Reproductive traits are of paramountimportance in an efficient lamb production system. In Croatia, values of reproductive traits of Romanov sheep are below the average expected for this breed, indicating the need for improvement of these traits to exploit the breed’s full reproductive potential. This study was conducted on 260 Romanov ewes located at a commercial Romanov sheep farm in Croatia. All ewes were kept under similar conditions, and reproductive management was based on accelerated lambing with continuous mating. Linear models with fixed effects were used to estimate the influence of year of birth/lambing, season of birth/lambing, ram, parity and litter type on litter size (LS), age at first lambing (AFL) and lambing interval (LI). Average LS was 2.11 ± 0.71, while the year of lambing and parity were the most important factors affecting this trait. The smallest litters were recorded after 1st parity (1.77 ± 0.06), and the largest after 5th parity (2.21 ± 0.08). AFL averaged 388.5 ± 72.4 days. All investigated non-genetic factors had a significant (P<0.05) influence on AFL, with year of birth as most important. The average LI was 241.2 ± 70.8 days. A significant (P<0.05) influence of all factors on LI was observed. Ewes lambing in spring (200.2 ± 7.9 days) or summer (190.5 ±6.6 days) had a significantly (P<0.05) shorter LI than ewes lambing in autumn (227.7 ± 8.3 days) or winter (237.2 ± 6.7 days). The longest LI was observed after the first parity (284.0 ±5.5 days). Average values for reproductive traits in the examined population of Romanov sheep were higher than those reported in the official Croatian database for breeding sheep. Non-genetic factors have a significant influence on the variation of reproductive traits of Romanov sheep, and therefore should be considered when assessing ewes’ reproductive performance.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46663216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to establish an experimental protocol for the artificial reproduction of royal carp ‘Cyprinus carpio’ with the hormonal induction of spawning. For this purpose, we conducted this study at an aquaculture farm in Khemis Meliana (Ain Defla, Algeria). The study included six royal carp broodstock, 4 females and 2 males. Hormone injection was performed in the dorsal muscle under the fin. The induction of carp was done successively in females by GnRH at a dose of 3 mg/kg and in males by hCG at a dose of 500 IU per kg weight. Fertilisation was done artificially by the dry method. After incubation of eggs, a binocular magnifying glass was used to check the condition of eggs and the development of the embryos over time. The results obtained show that the artificial reproduction of this species was possible, with success of ovulation and fertilisation, and hatching and growth of the larvae. Stripping was done after 24 h of hormonal stimulation at a temperature of 21°C. Disaggregation of eggs was performed efficiently with whole milk. The total number of eggs was 552,000 eggs with a weight of 1104 g. The average latency time was 22 hours. The hatching rate after 3 days of incubation on artificial spawning grounds at 21±1°C was 441,600 larvae (80%). Larvae food follow-up started from the 3rd day post-hatching with a combination natural food (rotifers) and artificial food. At the end of this experiment, we can conclude that it is possible to improve reproduction through the proper use of hormonal stimulation techniques and by improving feeding and abiotic factors that are dominant in fish farming.
{"title":"Artificial reproduction of royal carp (Cyprinus carpio)","authors":"A. Rouabah, N. Mimoune, D. Khelef, R. Kaidi","doi":"10.46419/vs.54.3.4","DOIUrl":"https://doi.org/10.46419/vs.54.3.4","url":null,"abstract":"The aim of this study was to establish an experimental protocol for the artificial reproduction of royal carp ‘Cyprinus carpio’ with the hormonal induction of spawning. For this purpose, we conducted this study at an aquaculture farm in Khemis Meliana (Ain Defla, Algeria). The study included six royal carp broodstock, 4 females and 2 males. Hormone injection was performed in the dorsal muscle under the fin. The induction of carp was done successively in females by GnRH at a dose of 3 mg/kg and in males by hCG at a dose of 500 IU per kg weight. Fertilisation was done artificially by the dry method. After incubation of eggs, a binocular magnifying glass was used to check the condition of eggs and the development of the embryos over time. The results obtained show that the artificial reproduction of this species was possible, with success of ovulation and fertilisation, and hatching and growth of the larvae. Stripping was done after 24 h of hormonal stimulation at a temperature of 21°C. Disaggregation of eggs was performed efficiently with whole milk. The total number of eggs was 552,000 eggs with a weight of 1104 g. The average latency time was 22 hours. The hatching rate after 3 days of incubation on artificial spawning grounds at 21±1°C was 441,600 larvae (80%). Larvae food follow-up started from the 3rd day post-hatching with a combination natural food (rotifers) and artificial food. At the end of this experiment, we can conclude that it is possible to improve reproduction through the proper use of hormonal stimulation techniques and by improving feeding and abiotic factors that are dominant in fish farming.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41355357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kristina Matković, Mario Ostović, Marija Batinjan, Ivana Sabolek, Sven Menčik, Željko Pavičić
Cilj je ovog našeg istraživanja bio pratiti, analizirati i usporediti mikroklimatske uvjete u stajama za goveda. Vrijednosti mikroklimatskih pokazatelja praćene su u ožujku i travnju tijekom četiri godine (2018. -2021.) u staji za junad u tovu i staji za mliječne krave. Rezultati istraživanja pokazali su smjer rasta temperature zraka i međupovezanost mikroklimatskih pokazatelja u objema stajama, a i veći broj bakterija u zraku u staji za junad u tovu, nego li u staji za mliječne krave. Dobiveni rezultati potvrđuju važnost analize mikroklimatskih uvjeta u stajamaza goveda te upućuju na moguću buduću potrebu za prilagodbom u tehnologiji njihova držanja.
{"title":"Analiza mikroklimatskih uvjeta u stajama za goveda","authors":"Kristina Matković, Mario Ostović, Marija Batinjan, Ivana Sabolek, Sven Menčik, Željko Pavičić","doi":"10.46419/vs.54.3.7","DOIUrl":"https://doi.org/10.46419/vs.54.3.7","url":null,"abstract":"Cilj je ovog našeg istraživanja bio pratiti, analizirati i usporediti mikroklimatske uvjete u stajama za goveda. Vrijednosti mikroklimatskih pokazatelja praćene su u ožujku i travnju tijekom četiri godine (2018. -2021.) u staji za junad u tovu i staji za mliječne krave. Rezultati istraživanja pokazali su smjer rasta temperature zraka i međupovezanost mikroklimatskih pokazatelja u objema stajama, a i veći broj bakterija u zraku u staji za junad u tovu, nego li u staji za mliječne krave. Dobiveni rezultati potvrđuju važnost analize mikroklimatskih uvjeta u stajamaza goveda te upućuju na moguću buduću potrebu za prilagodbom u tehnologiji njihova držanja.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42163607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Serhii Boianovskiy, L. Vygovska, V. Melnyk, A. Ushkalov, K. Rudnieva, L. Ishchenko, T. Mazur, V. Ushkalov
A significant number of microorganisms in natural and artificial environments exist in a structured formation – biofilm. This formation attaches to a certain surface, particularly the epithelium. The ability to form a similar structure has been observed in Pasteurella multocida, the causative agent of anthropozoonoses that affect domestic and wild animals, birds, companion animals and humans. The spectrum of pathogenetic action of P. multocida is wide and associated with the development of respiratory and multisystemic pathology, bacteraemia and other manifestations. Timely detection of P. multocida and treatment of the diseases it causes in farm and domestic animals is important to limit economic losses and improve social security. The main objective of this study was to determine the pathogenicity of P. multocida, its ability to form a biofilm, its resistance to antibiotics, and to identify the genes responsible for the formation of dermonecrotic toxin and biofilm formation. The paper presents the results of a study of 11 isolates of P. multocida: six isolates (54.5%) from rabbits, two isolates (18.2%) from dogs, two isolates (18.2%) from cats, and one isolate from pigs (9.2%). In all isolates, the gene ptfA was detected. This gene encodes the formation of type 4 fimbriae and participates in the formation of the biofilm, and the studied cultures in vitro formed a biofilm of different densities. The genome of eight isolates (72.7%) included the toxA gene (provides the formation of dermonecrotic toxin), while 45.4% of isolates had a complete set of the studied signs of pathogenicity, both in phenotypic (biofilm formation, mortality for laboratory animals) and genotypic (presence of toxA, ptfA) traits, and three isolates (27.3%) showed signs of multidrug resistance. The virulence of the toxA-negative isolates of P. multocida was lower than in toxA-positive isolates. The culture with the highest virulence (0.5x 101 CFU) and extreme resistance to antibiotics formed a biofilm of the highest density. The association of the gene in the biofilm-producing mechanism needs further evaluation, and further research is needed to identify the relationships between pathogens in Pasteurella multocida isolates from different species of animals and humans.
{"title":"Studija znakova patogenosti kod Pasteurella multocida, izolirane iz životinja različitih vrsta","authors":"Serhii Boianovskiy, L. Vygovska, V. Melnyk, A. Ushkalov, K. Rudnieva, L. Ishchenko, T. Mazur, V. Ushkalov","doi":"10.46419/vs.54.3.5","DOIUrl":"https://doi.org/10.46419/vs.54.3.5","url":null,"abstract":"A significant number of microorganisms in natural and artificial environments exist in a structured formation – biofilm. This formation attaches to a certain surface, particularly the epithelium. The ability to form a similar structure has been observed in Pasteurella multocida, the causative agent of anthropozoonoses that affect domestic and wild animals, birds, companion animals and humans. The spectrum of pathogenetic action of P. multocida is wide and associated with the development of respiratory and multisystemic pathology, bacteraemia and other manifestations. Timely detection of P. multocida and treatment of the diseases it causes in farm and domestic animals is important to limit economic losses and improve social security. The main objective of this study was to determine the pathogenicity of P. multocida, its ability to form a biofilm, its resistance to antibiotics, and to identify the genes responsible for the formation of dermonecrotic toxin and biofilm formation. The paper presents the results of a study of 11 isolates of P. multocida: six isolates (54.5%) from rabbits, two isolates (18.2%) from dogs, two isolates (18.2%) from cats, and one isolate from pigs (9.2%). In all isolates, the gene ptfA was detected. This gene encodes the formation of type 4 fimbriae and participates in the formation of the biofilm, and the studied cultures in vitro formed a biofilm of different densities. The genome of eight isolates (72.7%) included the toxA gene (provides the formation of dermonecrotic toxin), while 45.4% of isolates had a complete set of the studied signs of pathogenicity, both in phenotypic (biofilm formation, mortality for laboratory animals) and genotypic (presence of toxA, ptfA) traits, and three isolates (27.3%) showed signs of multidrug resistance. The virulence of the toxA-negative isolates of P. multocida was lower than in toxA-positive isolates. The culture with the highest virulence (0.5x 101 CFU) and extreme resistance to antibiotics formed a biofilm of the highest density. The association of the gene in the biofilm-producing mechanism needs further evaluation, and further research is needed to identify the relationships between pathogens in Pasteurella multocida isolates from different species of animals and humans.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48024756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ksenija Vlahović, Marko Samardžija, Gordana Gregurić Gračner, Marko Pećin, Hrvoje Capak, Nino Maćešić
Uspješnost osposobljavanja budućih doktora veterinarske medicine u izravnoj je vezi s kompetencijama nastavnika, odnosno svih sudionika u osposobljavanju doktorica/ doktora veterinarske medicine iz raznih područja, a koje obuhvaćaju znanje i vještine koje nastoje prenijeti, kao i njihove stavove. Stoga je važno usuglasiti zahtjeve struke u odnosu na kompetencije sudionika u profesionalnom osposobljavanju studenata veterinarske medicine. To se posebno odnosi na vještine koje studenti moraju usvojiti, a odnose se na specifične i generičke kompetencije za obavljanje profesionalnih dužnosti u širem europskom prostoru. Cilj ovog rada je predstaviti „Kompetencije prvoga dana“ (engl. Day One Competences, DOC), odnosno zacrtane nužne profesionalne vještine novih doktorica/doktora veterinarske medicine, kao i pobliže objasniti i olakšati razumijevanje temeljnih odrednica navedenog koncepta. DOC je usvojio Europski koordinacijski odbor za veterinarsku edukaciju (engl. European Coordination Committee on Veterinary Training, ECCVT), koji su osnovali Europska udruga ustanova za veterinarsku naobrazbu (engl. European Association of Establishments for Veterinary Education, EAEVE), Europski odbor za veterinarsku specijalizaciju (engl. European Board of Veterinary Specialisation,EBVS®) i Federacija europskih veterinara (engl. Federation of Veterinarians of Europe, FVE). DOC su detaljno opisane u dokumentu Standardni operativni postupak (SOP, 2019.) Europskog sustava evaluacije veterinarske izobrazbe (engl. European System of Evaluation of Veterinary Training, ESEVT) koji također djeluje unutar EAEVE-a.
{"title":"Temeljne odrednice okvira za stjecanje kompetencija i unaprjeđenje profesionalnih vještina na Veterinarskom fakultetu Sveučilišta u Zagrebu, Hrvatska","authors":"Ksenija Vlahović, Marko Samardžija, Gordana Gregurić Gračner, Marko Pećin, Hrvoje Capak, Nino Maćešić","doi":"10.46419/vs.54.3.9","DOIUrl":"https://doi.org/10.46419/vs.54.3.9","url":null,"abstract":"Uspješnost osposobljavanja budućih doktora veterinarske medicine u izravnoj je vezi s kompetencijama nastavnika, odnosno svih sudionika u osposobljavanju doktorica/ doktora veterinarske medicine iz raznih područja, a koje obuhvaćaju znanje i vještine koje nastoje prenijeti, kao i njihove stavove. Stoga je važno usuglasiti zahtjeve struke u odnosu na kompetencije sudionika u profesionalnom osposobljavanju studenata veterinarske medicine. To se posebno odnosi na vještine koje studenti moraju usvojiti, a odnose se na specifične i generičke kompetencije za obavljanje profesionalnih dužnosti u širem europskom prostoru. Cilj ovog rada je predstaviti „Kompetencije prvoga dana“ (engl. Day One Competences, DOC), odnosno zacrtane nužne profesionalne vještine novih doktorica/doktora veterinarske medicine, kao i pobliže objasniti i olakšati razumijevanje temeljnih odrednica navedenog koncepta. DOC je usvojio Europski koordinacijski odbor za veterinarsku edukaciju (engl. European Coordination Committee on Veterinary Training, ECCVT), koji su osnovali Europska udruga ustanova za veterinarsku naobrazbu (engl. European Association of Establishments for Veterinary Education, EAEVE), Europski odbor za veterinarsku specijalizaciju (engl. European Board of Veterinary Specialisation,EBVS®) i Federacija europskih veterinara (engl. Federation of Veterinarians of Europe, FVE). DOC su detaljno opisane u dokumentu Standardni operativni postupak (SOP, 2019.) Europskog sustava evaluacije veterinarske izobrazbe (engl. European System of Evaluation of Veterinary Training, ESEVT) koji također djeluje unutar EAEVE-a.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43893388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ksenija Šandor, S. Terzić, Ž. Mihaljević, M. Benič, I. Žarković, Miroslav Andrišić, Eleonora Perak Junaković
High performance liquid chromatography (HPLC) has proven to be an effective tool for examining the disposition kinetics of florfenicol (FF), a synthetic broad-spectrum antibiotic used to treat infectious diseases in veterinary medicine. Modification and optimisation of the protein precipitation (PPT) sample preparation procedure and HPLC with diode array detector (DAD) instrumental method were carried out to ensure conditions suitable for FF analyses in pig plasma samples. Stable supernatants with good plasma mean recoveries of FF (99.8% ± 0.7%RSD) were achieved using 1% v/v phosphoric acid solution in methanol and 10% w/v sodium chloride in aqueous solution. The PPT-HPLCDAD method’s detection limit of 0.004 μg/mL and quantification limit of 0.013 μg/mL provides high sensitivity for analyses of FF in plasma samples. In addition, the optimisation of method conditions resulted in shorter extraction and analysis time and less solvent consumption, which stresses the sustainability of this method in analytical chemistry. The optimised and validated PPT-HPLC-DAD method was applied in a comparative study of FF in pig plasma after administration of veterinary medicinal products. The study was conducted on fattening pigs following repeated intramuscular administration of two similar solutions for injection at a dose of 20 mg FF/kg bodyweight (test groups 1 and 2). The solutions for injection contained the same FF concentration, i.e., 300 mg/mL, but differed in excipients. The aim was to examine the influence of administrated solutions for injection on the extent of exposure to FF in pig plasma. The dynamics of kinetic profiles of FF in pig plasma from both treatments correspond to the FF kinetic profiles published in similar studies. However, differences were observed in the concentrations of FF, which were constant throughout the study, and statistical differences between the test groups were confirmed (P<0.05). Though these findings suggest the possible influence of excipients, a full comprehensive conclusion on the influence of administrated solutions for injection on FF exposure in pig plasma requires additional research.
{"title":"Analysis of florfenicol in pig plasma using a validated PPT-HPLC-DAD method","authors":"Ksenija Šandor, S. Terzić, Ž. Mihaljević, M. Benič, I. Žarković, Miroslav Andrišić, Eleonora Perak Junaković","doi":"10.46419/vs.54.3.8","DOIUrl":"https://doi.org/10.46419/vs.54.3.8","url":null,"abstract":"High performance liquid chromatography (HPLC) has proven to be an effective tool for examining the disposition kinetics of florfenicol (FF), a synthetic broad-spectrum antibiotic used to treat infectious diseases in veterinary medicine. Modification and optimisation of the protein precipitation (PPT) sample preparation procedure and HPLC with diode array detector (DAD) instrumental method were carried out to ensure conditions suitable for FF analyses in pig plasma samples. Stable supernatants with good plasma mean recoveries of FF (99.8% ± 0.7%RSD) were achieved using 1% v/v phosphoric acid solution in methanol and 10% w/v sodium chloride in aqueous solution. The PPT-HPLCDAD method’s detection limit of 0.004 μg/mL and quantification limit of 0.013 μg/mL provides high sensitivity for analyses of FF in plasma samples. In addition, the optimisation of method conditions resulted in shorter extraction and analysis time and less solvent consumption, which stresses the sustainability of this method in analytical chemistry. The optimised and validated PPT-HPLC-DAD method was applied in a comparative study of FF in pig plasma after administration of veterinary medicinal products. The study was conducted on fattening pigs following repeated intramuscular administration of two similar solutions for injection at a dose of 20 mg FF/kg bodyweight (test groups 1 and 2). The solutions for injection contained the same FF concentration, i.e., 300 mg/mL, but differed in excipients. The aim was to examine the influence of administrated solutions for injection on the extent of exposure to FF in pig plasma. The dynamics of kinetic profiles of FF in pig plasma from both treatments correspond to the FF kinetic profiles published in similar studies. However, differences were observed in the concentrations of FF, which were constant throughout the study, and statistical differences between the test groups were confirmed (P<0.05). Though these findings suggest the possible influence of excipients, a full comprehensive conclusion on the influence of administrated solutions for injection on FF exposure in pig plasma requires additional research.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49459118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ana Cristina Matos, Daniela Alves, Ana Cláudia Coelho, Manuela Matos, Luís Cardoso, Luís Figueira, Ana Sofia Soares, Sofia Saraiva
Dermatophytosis is a common dermatological infection, affecting humans, pets, domestic ruminants and wild animals. It is a cause for concern as it represents a risk to public and animal health. The aim of this study was to describe the occurrence of dermatophyte infection in wild mammals (road-killed and hunted) in Portugal. Fungal isolation was performed on the fur and scales of 101 wild mammals. Based on the observation of microstructures and colony morphology, fungal isolates were identified to the genus level. Dermatophytes were found in two of the 10 studied species: 3/51 (5.9%; 95% confidence interval [CI]: 0.0–12.3%) red fox (Vulpes vulpes), and 5/19 (2.6%; 95% CI: 0.0–9.8%) Egyptian mongoose (Herpestes ichneumon). These species represent different taxonomic families: Canidae and Viverridae and it is therefore important to infer the role of infection in these animals studied for dermatophytosis.
{"title":"Crvena lisica (Vulpes vulpes) i egipatski mungos (Herpestes ichneumon) kao prijenosnici dermatofita u Portugalu","authors":"Ana Cristina Matos, Daniela Alves, Ana Cláudia Coelho, Manuela Matos, Luís Cardoso, Luís Figueira, Ana Sofia Soares, Sofia Saraiva","doi":"10.46419/vs.54.3.2","DOIUrl":"https://doi.org/10.46419/vs.54.3.2","url":null,"abstract":"Dermatophytosis is a common dermatological infection, affecting humans, pets, domestic ruminants and wild animals. It is a cause for concern as it represents a risk to public and animal health. The aim of this study was to describe the occurrence of dermatophyte infection in wild mammals (road-killed and hunted) in Portugal. Fungal isolation was performed on the fur and scales of 101 wild mammals. Based on the observation of microstructures and colony morphology, fungal isolates were identified to the genus level. Dermatophytes were found in two of the 10 studied species: 3/51 (5.9%; 95% confidence interval [CI]: 0.0–12.3%) red fox (Vulpes vulpes), and 5/19 (2.6%; 95% CI: 0.0–9.8%) Egyptian mongoose (Herpestes ichneumon). These species represent different taxonomic families: Canidae and Viverridae and it is therefore important to infer the role of infection in these animals studied for dermatophytosis.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42709972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Daniela Herrera Vargas, Maricela Castaño Escobar, Henry Cardona Cadavid, Walter D. Cardona-Maya, Jose Manuel Mayorga-Torres, Jorge López Pérez, Daniela Arcila Davila
Evaluating the reproductive indicators in sheep breeding in Colombia, particularly males, is essential to achieve optimal production parameters. This study aimed to evaluate the semen quality of male goats through both conventional and functional seminal analysis. Semen samples from eight goats were collected and conventional (volume, appearance, colour, motility, vigour, and morphology) and functional (plasma membrane integrity, mitochondrial membrane potential, DNA integrity, lipid peroxidation, and reactive oxygen species production) seminal parameters were evaluated. The results showed an average scrotal circumference of 25.69 cm, seminal volume of 0.51 mL, and concentration of 1936 x 106 sperm/mL. The average individual motility was 63.37%, vigour was four, and the percentage of abnormalities was 7.75%. Individual motility was correlated with the mitochondrial membrane potential (r=0.840, P=0.009) and reactive oxygen species production (r=-0.91, P=0.001). The average of cells with high mitochondrial membrane potential was 52.94%, while 39.29% were necrotic cells, the DNA fragmentation index average was 12.5%, reactive oxygen species production was 38.68%, lipoperoxidation analysis was 7.33% on average, and the integrity of the membrane was 54.77%. The results establish the parameters for the semen from Antioquia goats and confirm that goat semen is of good quality and could be used during artificial insemination programs.
评估哥伦比亚绵羊养殖的繁殖指标，特别是雄性绵羊，对于实现最佳生产参数至关重要。本研究旨在通过常规精液分析和功能性精液分析来评价雄山羊的精液质量。采集了8只山羊的精液样本，并评估了常规（体积、外观、颜色、运动性、活力和形态）和功能（质膜完整性、线粒体膜电位、DNA完整性、脂质过氧化和活性氧产生）精液参数。结果显示，平均阴囊周长为25.69厘米，精液体积为0.51毫升，精子浓度为1936 x 106个/mL。平均个体活力为63.37%，活力为4，异常率为7.75%。个体活力与线粒体膜电位（r=0.840，P=0.009）和活性氧产生（r=-0.91，P=0.001）相关。线粒体膜电位高的细胞平均为52.94%，而坏死细胞为39.29%，DNA断裂指数平均为12.5%，活性氧产生率为38.68%，脂质过氧化物分析平均为7.33%，膜完整性为54.77%。
{"title":"Konvencionalna i funkcionalna procjena sjemena mužjaka mliječnih koza","authors":"Daniela Herrera Vargas, Maricela Castaño Escobar, Henry Cardona Cadavid, Walter D. Cardona-Maya, Jose Manuel Mayorga-Torres, Jorge López Pérez, Daniela Arcila Davila","doi":"10.46419/vs.54.2.1","DOIUrl":"https://doi.org/10.46419/vs.54.2.1","url":null,"abstract":"Evaluating the reproductive indicators in sheep breeding in Colombia, particularly males, is essential to achieve optimal production parameters. This study aimed to evaluate the semen quality of male goats through both conventional and functional seminal analysis. Semen samples from eight goats were collected and conventional (volume, appearance, colour, motility, vigour, and morphology) and functional (plasma membrane integrity, mitochondrial membrane potential, DNA integrity, lipid peroxidation, and reactive oxygen species production) seminal parameters were evaluated. The results showed an average scrotal circumference of 25.69 cm, seminal volume of 0.51 mL, and concentration of 1936 x 106 sperm/mL. The average individual motility was 63.37%, vigour was four, and the percentage of abnormalities was 7.75%. Individual motility was correlated with the mitochondrial membrane potential (r=0.840, P=0.009) and reactive oxygen species production (r=-0.91, P=0.001). The average of cells with high mitochondrial membrane potential was 52.94%, while 39.29% were necrotic cells, the DNA fragmentation index average was 12.5%, reactive oxygen species production was 38.68%, lipoperoxidation analysis was 7.33% on average, and the integrity of the membrane was 54.77%. The results establish the parameters for the semen from Antioquia goats and confirm that goat semen is of good quality and could be used during artificial insemination programs.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":"18 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41271290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. R. Meklati, A. Meribai, M. Ben-Mahdi, M. Abou-Mustapha, N. Yezli-Meklati, T. Lyazidi, Y. Titouche
The uncontrolled use of antimicrobials in veterinary medicine may result in the presence of their residues in food matrices of animal origin such as milk. The aim of this study was to examine the physicochemical quality and to detect antibiotic residues (β-lactams and tetracyclines) in milk, as their presence has resulted in the rejection of significant quantities of milk for marketing. The study was conducted on 274 milk samples delivered to three milk-processing units located in the suburbs of Algiers and surrounding cities (Blida and Boumerdes). Antibiotic residue detection was performed using a fast screening test (BetaStar® Combo), with a reading result within five minutes. The results indicated that the milk processing industry of Blida had the highest rejected milk volumes (43.4%), followed by Algiers (24%) and Boumerdes (19.1%). Non-compliance of milk acidity was the first cause for milk rejection (47.6 %), followed by the presence of antibiotic residues (26.8%), and incompliance with requirements for density (13.4%) and fat levels (12.2%). The study revealed 22 positive cases (8.03%) of antibiotic residues. Among these, 90.91% were positive for β-lactams, and only 9.09% for tetracyclines. All tetracyclines cases and half of the β-lactams cases were found in the Blida milk processing company, indicating that the good practice of milk production and collection must be strengthened in that company.
{"title":"Fizikalno-kemijske karakteristike i detekcija rezidua antibiotika u sirovom kravljem mlijeku isporučenom u tri industrije za preradu mlijeka u Alžiru","authors":"F. R. Meklati, A. Meribai, M. Ben-Mahdi, M. Abou-Mustapha, N. Yezli-Meklati, T. Lyazidi, Y. Titouche","doi":"10.46419/vs.54.2.2","DOIUrl":"https://doi.org/10.46419/vs.54.2.2","url":null,"abstract":"The uncontrolled use of antimicrobials in veterinary medicine may result in the presence of their residues in food matrices of animal origin such as milk. The aim of this study was to examine the physicochemical quality and to detect antibiotic residues (β-lactams and tetracyclines) in milk, as their presence has resulted in the rejection of significant quantities of milk for marketing. The study was conducted on 274 milk samples delivered to three milk-processing units located in the suburbs of Algiers and surrounding cities (Blida and Boumerdes). Antibiotic residue detection was performed using a fast screening test (BetaStar® Combo), with a reading result within five minutes. The results indicated that the milk processing industry of Blida had the highest rejected milk volumes (43.4%), followed by Algiers (24%) and Boumerdes (19.1%). Non-compliance of milk acidity was the first cause for milk rejection (47.6 %), followed by the presence of antibiotic residues (26.8%), and incompliance with requirements for density (13.4%) and fat levels (12.2%). The study revealed 22 positive cases (8.03%) of antibiotic residues. Among these, 90.91% were positive for β-lactams, and only 9.09% for tetracyclines. All tetracyclines cases and half of the β-lactams cases were found in the Blida milk processing company, indicating that the good practice of milk production and collection must be strengthened in that company.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42149394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Noufel Ouail Marouf, N. Mimoune, Aymène Benchabane, D. Khelef, R. Kaidi
Three-dimensional printing, which appeared in the 1980s and has been steadily improving ever since, is a new and very promising technology. Thanks to its unique process of depositing material layer by layer, it differentiates itself from the rest of the traditional methods of modeling by molding and removing material. Modeling by adding material allows 3D printing to create parts with very complex geometries, and even with unprecedented precision. This last characteristic allows it to be used in many sectors including aviation, automotive, production but also science, education and medicine. With regard to these latter fields of application, printing by adding material is for some authors a real revolution. For modeling learning and training mockups, manufacturing custom prostheses, or printing biological and functional organs, the range of possible uses for 3D printing seems immense and very promising. In this study, we invite you to discover the main applications of the 3D printer in veterinary medicine
{"title":"3d printer uses in veterinary medicine","authors":"Noufel Ouail Marouf, N. Mimoune, Aymène Benchabane, D. Khelef, R. Kaidi","doi":"10.46419/vs.54.2.9","DOIUrl":"https://doi.org/10.46419/vs.54.2.9","url":null,"abstract":"Three-dimensional printing, which appeared in the 1980s and has been steadily improving ever since, is a new and very promising technology. Thanks to its unique process of depositing material layer by layer, it differentiates itself from the rest of the traditional methods of modeling by molding and removing material. Modeling by adding material allows 3D printing to create parts with very complex geometries, and even with unprecedented precision. This last characteristic allows it to be used in many sectors including aviation, automotive, production but also science, education and medicine. With regard to these latter fields of application, printing by adding material is for some authors a real revolution. For modeling learning and training mockups, manufacturing custom prostheses, or printing biological and functional organs, the range of possible uses for 3D printing seems immense and very promising. In this study, we invite you to discover the main applications of the 3D printer in veterinary medicine","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49037489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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