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Seroprevalence of Aujeszky's disease in the domestic pig population in the Republic of Croatia from 2013 to 2021 2013年至2021年克罗地亚共和国家养猪群中奥耶斯基病的血清患病率
Q3 Veterinary Pub Date : 2023-02-06 DOI: 10.46419/vs.54.5.9
Ž. Acinger-Rogić, Marina Pavlak, L. Jemeršić
Aujeszky's disease is a viral disease of animals, primarily pigs, which are considered to be the natural hosts. It is endemic in many parts of the world, but there are areas and countries that have successfully eradicated this disease. Due to mandatory control measures and trade barriers, Aujeszky's disease causes significant economic losses. Although, it has been eradicated in most European Union Member States, there are still areas and countries where it persists. In the Republic of Croatia, the disease has not yet been eradicated from the domestic pig population, although surveillance and eradication has been carried out since 2013. This paper presents the results of serological testing of domestic pigs for Aujeszky's disease in the period from 2013 to 2021. The prevalence of Aujeszky's disease at the farm level by year in the study period ranged from 4.66% (CI 95%, 4.39-4.95) to 0.67% (CI 95%, 0.35-1.28). Since 2016, prevalence was at an average level of 1.26% (CI 95%, 0.67-1.95%). The highest prevalence 10.28% (CI 95%, 9.15-11.53) was found in Varaždin County, and the lowest 0.14 % (CI 95%, 0.07-0.31) in Krapina-Zagorje County.
奥杰斯基病是一种动物病毒性疾病,主要是猪,被认为是自然宿主。它在世界许多地方流行,但有些地区和国家已经成功地根除了这种疾病。由于强制性控制措施和贸易壁垒,奥杰斯基病造成了重大的经济损失。尽管在大多数欧洲联盟成员国已经根除了该病,但仍有一些地区和国家存在该病。在克罗地亚共和国,尽管自2013年以来开展了监测和根除工作,但该疾病尚未从家养猪群中根除。本文介绍了2013 - 2021年家猪奥耶斯基病血清学检测结果。在研究期间,农场水平上的奥耶斯基病的年患病率从4.66% (CI 95%, 4.39-4.95)到0.67% (CI 95%, 0.35-1.28)不等。2016年以来,患病率平均为1.26% (CI 95%, 0.67-1.95%)。Varaždin县患病率最高,为10.28% (CI 95%, 9.15 ~ 11.53), Krapina-Zagorje县患病率最低,为0.14% (CI 95%, 0.07 ~ 0.31)。
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引用次数: 0
Učinci radiofrekvencijskog elektromagnetskog zračenja na mušku plodnost
Q3 Veterinary Pub Date : 2023-02-06 DOI: 10.46419/vs.54.5.6
Ivona Žura Žaja, Ivan Folnožić, Jadranka Pejaković Hlede, Marko Samardžija, Anamaria Sluganović, Nikolino Žura, Silvijo Vince, Suzana Milinković-Tur, Marinko Vilić, Iva Butković, Paulina Martinec
Elektromagnetsko se zračenje emitira iz prirodnog okruženja, ali i uporabom industrijskih i svakodnevnih uređaja za bežičnu komunikaciju, stoga su ljudski i životinjski organizmi stalno izloženi zračenju. Tijekom posljednjih godina, zbog brzog tehnološkog napretka, elektromagnetsko je zračenje iz umjetnih izvora premašilo je vrijednosti zračenja prirodnog podrijetla. Opća zabrinutost svih nas, zbog svevećeg broja uređaja (mobilni telefoni, prijenosna računala, Wi-Fi-ja i mikrovalnih pećnica), koji koriste radiofrekvencijsko elektromagnetsko zračenje (RF-EMZ) opravdana je zbog sve brojnijih dokaza o njihovoj štetnosti na žive organizme. Suvremeni uređaji moderne tehnologije emitiraju radiofrekvencijske elektromagnetske valove malih frekvencija koje ljudsko i životinjsko tijelo apsorbira što može imati potencijalne štetne učinke na: mozak, srce, endokrini sustav i reproduktivnu funkciju. Muški je reproduktivni sustav jedno od najosjetljivijih tkiva na RF-EMZ-e. Tako je primjerice, iz trenutno dostupnih studija provedenih in vitro i in vivo, jasno da RF-EMZ-e ima štetne učinke na spermatogenezu, odnosno kakvoću ejakulata ljudi i životinja – broj spermija u ejakulatu, preživljavanje, morfologiju i gibljivost spermija - utječu na stanični metabolizam i endokrini sustav i može prouzročiti genotoksičnost, genomsku nestabilnost i oksidativni stres, a to može prouzročiti neplodnost. Štetni učinci RFEMZ-a dijele se na: toplinske i netoplinske. Većinanegativnih bioloških učinaka pripisuje se netoplinskim učincima, a toplinski se učinci nastali RF-EMZ mobilnog telefona, smatraju manje štetnima. Zbog stvaranja prevelike količine reaktivnih kisikovih spojeva u muškom spolnom sustavu hipertermija skrotuma i povećani oksidativni stres mogu biti ključni mehanizmi putem kojih RF-EMZ-e utječe na plodnost muškaraca. Navedeni su i negativni učinci povezani s vremenom korištenja, ponajprije mobilnog telefona. Stoga je cilj ovoga preglednog rada opisati neke od učinaka RF-EMZ-a na muški spolni sustav.
电磁辐射来自自然环境,也来自工业和日常无线通信设备,因此人类和动物生物体不断暴露在辐射中。在过去的几年里,由于技术的快速进步,来自人工源的电磁辐射已经超过了自然源辐射的价值。由于大量使用射频电磁辐射(RF-EMZ)的设备(手机、便携式电脑、Wi-Fi和微波炉),我们所有人的整体担忧是有理由的,因为有更多证据表明它们对生物体有害。现代技术的发展方向是:mozak、srce、endokrini sustav i reproduktivnu funkciju。雄性生殖系统是RF-EMZ中最敏感的组织之一。例如,在体外和体内,RF-EMZ对精子发生有不利影响,包括人类和动物的射精类型——射精中的精子数量、精子的存活率、形态和生育能力——影响细胞代谢和内分泌系统,并可能导致遗传毒性、遗传不稳定和氧化应激。这可能会导致失败。RFEMZ的不良反应分为:发热和奈托普林。大多数负面生物效应归因于netoplin效应,而RF-EMZ手机的热效应被认为危害较小。鉴于男性性体温过高和氧化应激增加会产生过量的人类活性氧化合物,RF-EMZ可用于影响男性生育能力的关键机制。使用时间的负面影响也被指出,至少在使用手机之前。因此,本综述的目的是描述RF-EMZ对男性性别系统的一些影响。
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引用次数: 0
Radiološki pregled zuba kunića 兔牙齿的放射学检查
Q3 Veterinary Pub Date : 2023-02-06 DOI: 10.46419/vs.54.5.5
Marko Mitrovic, Ana U. Todorović, Zoran Vrbanac, Aleksandar Janevski, M. Lazarević Macanović
Patološka su stanja zuba kunića u svakodnevnoj kliničkoj praksi vrlo česta i često rezultiraju poremećajem u funkciji organa digestivnog i respiratornog sustava, a mogu imati i letalan ishod. Da bi se omogućila pravovremena primena terapije i spriječila pojava komplikacija, posebnu pažnju treba usmjeriti na ranu dijagnostiku. Radiološka dijagnostika predstavlja jednostavan, brz i nezaobilazan postupak u procjeni stanja zubi. Za razliku od drugih vrsta sisavaca, radiološki pregled zuba kunića se odlikuje određenim osobitostima koje se, prije svega, očituju u tehnikama snimanja i interpretaciji rendgenskih snimaka koji su detaljno prikazani u ovom radu.
巡逻异常在日常临床实践中非常常见,通常会导致消化和呼吸系统器官功能紊乱,也可能导致致命后果。Da bi se omogućila pravovremena terapije i spriječila pojava komplikacija,posebnu pažnju treba usmjeriti na ranu dijagnostiku。放射学诊断是牙齿状态评估中一个简单、快速且不可避免的过程。与其他哺乳动物不同,兔子牙齿的放射学检查是由本工作中详细介绍的视频和X射线解释技术中首次读取的某些特征决定的。
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引用次数: 0
Entropy and its significance in transport of ions through the cell membrane 熵及其在离子通过细胞膜运输中的意义
Q3 Veterinary Pub Date : 2023-02-06 DOI: 10.46419/vs.54.5.8
Selim Pašić, Nato Popara, Antea Klobučar, D. Cvitković, M. Vilić
The aim of this paper is to present the concept of entropy in a simple way and to show its key importance in the transport processes of ions through the cell membrane using the latest discoveries in biophysics. Using a real-life example, we show how processes within a system lead to an increase in entropy. We also show how this entropy increase is directly related to the irreversibility of the process, and how it defines the arrow of time (direction of the flow of time). Using an abstract example, we clarify the meaning of the concept of disorder in a system, which is often used in defining entropy by connecting it with the number of microstates that realise a macroscopic state of a system. The importance of entropy in transport processes of ions through the cell membrane is considered. We show that passive transport processes through the cell membrane are the result of an entropy increase in the cell membrane-transported substance system. A model of active ion transport through the cell membrane following Rubi et al. (2017) is presented. The force that transports ions through the channel in the transport protein arises due to the entropy gradient formed along the transport channel, which is a consequence of the funnel shape of the channel. The entropic force is proportional to the ratio of the ion-available cross-sections of the exit and entrance surface of the channel. That means that only a very funnel-shaped channel can produce a sufficiently large force on the ions to overcome the concentration gradient of the substance. We analyse the final result for the force of entropy in the limits of a very wide and very narrow channel and find that the entropic force is proportional to the ratio of the areas of the exit to entrance surfaces of the channel, i.e., when the channel is very wide, while it becomes high as the width of the channel tends to the ion diameter, i.e., when the channel is very narrow. We explicitly explain how the presented model solves several fundamental questions about the active transport of substances: how is energy, a scalar quantity, converted into the directional motion of the ion (a vector quantity), how does energy drive ions considering that the point of release of energy is far from the point of binding of an ion in a transport protein and finally, how does energy, which is released in a very limited space, transport the ions over a very large spatial scale.
本文的目的是用一种简单的方式来介绍熵的概念,并利用生物物理学的最新发现来说明熵在离子通过细胞膜的运输过程中的关键重要性。通过一个现实生活中的例子,我们展示了系统中的过程是如何导致熵的增加的。我们还展示了熵的增加如何与过程的不可逆性直接相关,以及它如何定义时间之箭(时间流动的方向)。通过一个抽象的例子,我们澄清了系统中无序概念的含义,该概念通常用于通过将熵与实现系统宏观状态的微观状态的数量联系起来来定义熵。考虑了离子在细胞膜上的传递过程中熵的重要性。我们表明,通过细胞膜的被动运输过程是细胞膜运输物质系统熵增加的结果。Rubi et al.(2017)提出了一个通过细胞膜的活性离子运输模型。在运输蛋白中,由于沿着运输通道形成的熵梯度而产生通过通道运输离子的力,这是通道漏斗形状的结果。熵力与通道出口和入口表面的离子可用横截面的比例成正比。这意味着只有一个漏斗状的通道才能对离子产生足够大的力来克服物质的浓度梯度。我们分析了在非常宽和非常窄的通道极限下的熵力的最终结果,发现熵力与通道出口和入口表面的面积之比成正比,即当通道非常宽时,而当通道宽度趋向于离子直径时,即当通道非常窄时,熵力变得很高。我们明确地解释了模型解决了几个基本问题的主动运输物质:能源,如何一个标量,转化成离子的定向运动(矢量),如何考虑到能源驱动离子的能量释放点远从绑定的离子运输蛋白质和最后,能源,如何发布在一个非常有限的空间,运输离子在一个规模非常大的空间。
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引用次数: 0
Pasteurellosis: A significant bacterial disease in rabbit production 巴氏杆菌病:兔生产中的一种重要细菌性疾病
Q3 Veterinary Pub Date : 2023-02-06 DOI: 10.46419/vs.54.5.1
W. A. Abd El-Ghany
Rabbits are susceptible to various respiratory affections. Pasteurellosis, caused by P. multocida, is regarded as one of the most important bacterial diseases of rabbits. The disease is characterised by chronic mucopurulent respiratory affection (snuffles) or more acute and subacute bronchopneumonia leading to high mortality and severe devastating losses in rabbit production. Moreover, pasteurellosis is associated with septicaemia, abscesses, otitis media, and nervous and reproductive disorders. P. multocida is widely distributed worldwide and mostly affects rabbits from 4 to 8 weeks of life. Infection with P. multocida usually occurs by indirect or direct contact, chiefly via aerosol. Moreover, the presence of other stressors can aggravate the severity of infection. The gold standard for the diagnosis of pasteurellosis is traditional isolation and identification methods. However, molecular techniques are used now for rapid detection of P. multocida and its virulence genes. Prevention is based on the application of hygienic methods, vaccination, and treatment using various antimicrobials. This review article gives an overview of pasteurellosis in rabbits regarding disease incidence, susceptibility and transmission, signs and lesions, laboratory diagnosis, and the prevention and control methods.
兔子容易受到各种呼吸道疾病的影响。由多杀性巴氏杆菌引起的巴氏杆菌病被认为是家兔最重要的细菌性疾病之一。该疾病的特点是慢性黏液脓性呼吸道感染(鼻塞)或更严重的急性和亚急性支气管肺炎,导致高死亡率和严重的兔子生产损失。此外,巴氏杆菌病与败血症、脓肿、中耳炎、神经和生殖障碍有关。多杀性巴氏杆菌广泛分布于世界各地,主要影响4至8周的兔子。多杀性巴氏杆菌的感染通常通过间接或直接接触发生,主要是通过气溶胶。此外,其他压力源的存在会加重感染的严重程度。巴氏杆菌病诊断的金标准是传统的分离鉴定方法。然而,分子技术现在被用于快速检测多杀性巴氏杆菌及其毒力基因。预防是基于卫生方法的应用、疫苗接种和使用各种抗菌药物的治疗。本文综述了兔巴氏杆菌病的发病率、易感性和传播、体征和病变、实验室诊断以及预防和控制方法。
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引用次数: 0
A comparative study of the arthropod fauna in stork, barn swallow and common pigeon nests 鹳、燕和鸽子巢中节肢动物区系的比较研究
Q3 Veterinary Pub Date : 2023-02-06 DOI: 10.46419/vs.54.5.7
Rima Sadeddine, S. Righi, Karim Daghri, K. Saidani, Ahmed Benakhala
An analysis was conducted on the arthropod fauna collected from stork, swallow, and pigeon nests during summer 2018 in three regions of north-eastern Algeria (Guelma, El-Tarf, and Annaba). A total of 40 nests was examined, of which 58% were infested with arthropods. Arthropods were less abundant and species rich in swallow nests than in pigeon or stork nests, where a greater dominance of mesostigmatic mites (78.71% and 72.41%, respectively) was found. Dermanyssus gallinae was the most abundant (33.05%) and most frequent (73.33%) mesostigmatic mite in pigeon nests, while uropodina mites were dominant (41.37%) in white stork nests with a frequency of 76.92% of the nests analysed. In swallow nests, the following arthropods were found: Psocodea, Hemiptera, Hymenoptera and Coleoptera belonging to the class Insecta and Isopoda belonging to the class Malacostraca. This is the first comparative survey of arthropods occupying the nests of these bird species in northern Algeria. It is anticipated that these data, that highlight the richness of the arthropod fauna in the nests of these bird species, will motivate further research aimed at characterising the arthropod community in different bird nests and determining the nature of the relationships between them.
对2018年夏季在阿尔及利亚东北部三个地区(Guelma、El Tarf和Annaba)从鹳、燕子和鸽子窝中采集的节肢动物群进行了分析。共检查了40个巢穴,其中58%的巢穴受到节肢动物的侵扰。燕窝中的节肢动物数量较少,物种丰富,而鸽子窝或鹳窝中的中蜱螨占更大的优势(分别为78.71%和72.41%)。在鸽巢中,鸡鳞螨数量最多(33.05%),最常见(73.33%),而在白鹳巢中,尿足螨占主导地位(41.37%),频率为76.92%。在燕窝中,发现了以下节肢动物:昆虫纲的Psocodea、半翅目、膜翅目和鞘翅目,以及马六甲纲的Isopoda。这是阿尔及利亚北部首次对占据这些鸟类巢穴的节肢动物进行比较调查。预计这些数据突出了这些鸟类巢穴中节肢动物区系的丰富性,将推动进一步的研究,以表征不同鸟巢中的节肢动物群落,并确定它们之间关系的性质。
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引用次数: 0
First report of a cutaneous and shell mycosis in an Ouachita Map Turtle (Graptemys ouachitensis, Cagle, 1953) by Fusarium solani 首次报道了由梭氏镰刀菌引起的瓦奇塔龟皮肤和壳真菌病
Q3 Veterinary Pub Date : 2023-02-06 DOI: 10.46419/vs.54.5.3
Andreia Garcês, P. Brilhante-Simões, D. Duque, Filipe Sampaio, Augusto Silva, Ricardo Lopes, Delsy Salinas, Helder Fernandes
An adult Ouachita Map Turtle (Graptemys ouachitensis) was presented for consultation with multifocal, erosive and ulcerative lesions, multiple fissures on the plastron and shell, and dermatitis on the members and head. Samples of shell scrapings were collected for routine microbial culturing and Fusarium solani was isolated from the samples. To the author’s knowledge, this is the first reported case of F. solani associated with cutaneous and shell mycosis in this turtle species.
一只成年的Ouachita地图龟(Graptemys ouachitensis)因多灶性、侵蚀性和溃疡性病变、质体和外壳上的多处裂缝以及成员和头部的皮炎而接受会诊。收集壳屑样品用于常规微生物培养,并从样品中分离出镰刀菌。据作者所知,这是第一例报道的龙葵与该龟种的皮肤和外壳真菌病相关的病例。
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引用次数: 1
Diversity and prevalence of Salmonella spp. in gulls caught at a landfill, Zagreb, Croatia 克罗地亚萨格勒布垃圾填埋场捕获的海鸥中沙门氏菌的多样性和流行率
Q3 Veterinary Pub Date : 2023-02-06 DOI: 10.46419/vs.54.5.2
B. Ječmenica, A. Humski, Louie Thomas Taylor, B. Šimpraga, F. Krstulović, Tajana Amšel Zelenika, L. Jurinović
Gulls are a group of seabirds distributed worldwide that are an important reservoir of Salmonella spp. Salmonellosis is the second most commonly reported gastrointestinal infection in humans, and understanding the role wild birds have in spreading Salmonella can help to improve the health of humans and domestic animals. The mobility and migration capacity of gulls makes them an interesting group for research given their potential role in spreading pathogens. This paper presents the diversity and prevalence of Salmonella spp. in different gull species caught at a landfill in Zagreb in the winter months over a nine-year period from 2014-2022. In total, 1083 cloacal swabs were sampled from six gull species: Black-headed Gull (Larus ridibundus), Yellow-legged Gull (L. michahellis), Caspian Gull (L. cachinnans), Common Gull (L. canus), Lesser Black-back Gull (L. fuscus) and Herring Gull (L. argentatus). The prevalence of Salmonella was 5.82%, and 16 Salmonella serotypes were identified; S. Typhimurium had the highest prevalence (47.62%) followed by S. Enteritidis (12.69%) and S. Infantis (9.52%). To date, 82 Salmonella serotypes have been isolated in research on gulls in Europe, with S. Typhimurium as the most common, followed by S. Agona and S. Enteritidis. In this study, we found three serotypes not previously reported in gulls, S. Yalding, S. Reading and one with the antigenic formula O:17; H:z10; H:e,n,x,z15 (IIIb).
海鸥是分布在世界各地的一群海鸟,是沙门氏菌的重要宿主。沙门氏菌病是人类第二常见的胃肠道感染,了解野生鸟类在传播沙门氏菌方面的作用有助于改善人类和家畜的健康。海鸥的机动性和迁徙能力使其成为一个有趣的研究群体,因为它们在传播病原体方面具有潜在作用。本文介绍了2014年至2022年九年期间,萨格勒布垃圾填埋场冬季捕获的不同海鸥中沙门氏菌的多样性和流行率。总共从六种海鸥中采集了1083个泄殖腔拭子:黑头海鸥(Larus ridibundus)、黄腿海鸥(L.michahellis)、里海海鸥(L.cachinnans)、普通海鸥(L.canus)、小黑背海鸥(L.fuscus)和鲱鱼海鸥(L.argentatus)。沙门氏菌的患病率为5.82%,共鉴定出16种沙门氏菌血清型;鼠伤寒沙门氏菌的患病率最高(47.62%),其次是肠炎沙门氏菌(12.69%)和因凡特氏菌(9.52%)。迄今为止,在欧洲对海鸥的研究中已分离出82种沙门氏菌血清型,其中鼠伤寒沙门氏菌最常见,其次是阿戈纳沙门氏菌和肠炎沙门氏杆菌。在这项研究中,我们发现了三种以前没有在海鸥中报道过的血清型,即雅尔丁S.Yalding、雷丁S.Reading和一种抗原式为O:17的血清型;H: z10;H: e,n,x,z15(IIIb)。
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引用次数: 0
Pathogen presence in cottage cheese sold for public consumption at Sarajevo Canton markets 在萨拉热窝坎顿市场出售供公众食用的白干酪中发现病原体
Q3 Veterinary Pub Date : 2022-12-30 DOI: 10.46419/vs.54.4.7
Velma Rebić, M. Aljičević, V. Landeka, Džanela Mešinović, Amina Karić
Cottage cheese is the largest segment of the dairy market and is most often consumed as a fresh food. The microbiological quality of domestic cottage cheese can pose a problem for public health. Cottage cheese belongs to a group of foods having a potential public health risk. The aim of this study was to conduct microbiological research to determine the level of sanitary safety of cottage cheese types acquired from the most frequents markets of Sarajevo Canton (Bosnia and Herzegovina). Of the total (n=40) analysed cheese types, 22 samples (55%) proved to be meet sanitary requirements, while 18 samples (45%) did not meet microbiological quality, pursuant to the microbiological criteria for cheese stipulated by the National Regulation of Microbiological Criteria B&H and Guidelines for Microbiological Food Criteria B&H. The study included 24 samples of pasteurised and 16 samples of unpasteurised milk. However, five cheese samples (20.8%) from pasteurised milk, and 13 samples (81.3%) from unpasteurised milk were non-compliant. Microbiological analysis was conducted for compulsory and several recommended microorganisms: Salmonella spp., Listeria monocytogenes, coagulase positive staphylococci and other species (Staphylococcus aureus), Escherichia coli, and yeasts and moulds. Our findings confirm that the most common bacteria were S. aureus and E. coli. Also, yeasts and moulds were detected but were within the permissible concentrations. Salmonella spp. and L. monocytogenes were not detected in any of the examined samples.
白软干酪是乳制品市场中最大的一部分,通常作为新鲜食品食用。国产白软干酪的微生物质量可能对公众健康构成问题。白软干酪属于一组对公众健康有潜在风险的食物。本研究的目的是进行微生物学研究,以确定从萨拉热窝州(波斯尼亚和黑塞哥维那)最常见的市场获得的白干酪类型的卫生安全水平。根据《国家微生物标准规范(B&H)》和《微生物食品标准指南(B&H)》规定的奶酪微生物标准,在分析的40种奶酪中,22种样品(55%)符合卫生要求,18种样品(45%)不符合微生物质量要求。该研究包括24份巴氏消毒牛奶样本和16份未经巴氏消毒牛奶样本。然而,来自巴氏消毒牛奶的5个奶酪样品(20.8%)和来自未巴氏消毒牛奶的13个样品(81.3%)不符合要求。对强制性微生物和几种推荐微生物进行微生物学分析:沙门氏菌、单核增生李斯特菌、凝固酶阳性葡萄球菌等种(金黄色葡萄球菌)、大肠杆菌、酵母和霉菌。我们的研究结果证实,最常见的细菌是金黄色葡萄球菌和大肠杆菌。此外,检测到酵母和霉菌,但在允许的浓度范围内。在所有检测样品中均未检出沙门氏菌和单核增生乳杆菌。
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引用次数: 1
Retrospektivna procjena učinka nintedaniba u mišjem modelu plućne fibroze
Q3 Veterinary Pub Date : 2022-12-30 DOI: 10.46419/vs.54.4.4
Željka Anzulović Šanta, Anja Ognjenović, F. Božić, Boška Hrvačić, Ines Glojnarić, Snježana Čužić, Maja Antolić
Pulmonary function tests (PFTs) routinely implemented in clinics are the first step in the diagnosis of idiopathic pulmonary fibrosis. Evaluation of PFTs in the mouse model of pulmonary fibrosis accompanied by histological readouts may improve the clinical predictability of new therapeutic candidates. Forced vital capacity (FVC) is considered the most predictive of restrictive pulmonary disorders. This study aimed to test the improvement of PFT in mice lung fibrosis induced by treatment with an approved substance nintedanib, considered the gold standard. The hypothesis that treatment in animal models will demonstrate similar effects as in humans in the most relevant clinical outcomes was tested. Two experimental designs were enrolled in this study, a preventive regimen, with treatment initiation from the day of the challenge; and a therapeutic regimen, starting on day 7 postchallenge when fibrotic changes are present in the lungs. Experiments were terminated at two different time points, at 14 and 21 days postchallenge. C57BL/6 mice were administered with bleomycin (BLM) intranasally and treated with nintedanib from day 0 to day 14 or from day 7 until day 21. Fourteen or 21 days after the BLM challenge, PFTs were assessed using the in vivo invasive lung function measurement system Buxco® Pulmonary Function Test (PFT) (DSI™, New Brighton, USA). Histological evaluation was performed as a modified Ashcroft score. The bleomycin challenge induced a significant decrease of FVC in both experiments. However, nintedanib treatment given in both regimens significantly improved lung functionality. These findings were confirmed with histological analysis of the Ashcroft scoring system, modified by Matsuse. In conclusion, a good correlation between functional test parameters and the clinical effect of nintedanib was shown in both experiments: the preventive regimen was sampled 14 days post-challenge and the therapeutic regimen 21 days post-challenge. Based on these findings, the implementation of PFTs could be a good platform to increase the translational value of the model and potential new treatments.
临床常规肺功能测试是诊断特发性肺纤维化的第一步。在伴有组织学读数的肺纤维化小鼠模型中评估PFTs可以提高新的候选治疗药物的临床可预测性。强迫肺活量(FVC)被认为是最能预测限制性肺部疾病的指标。本研究旨在测试PFT对经批准的物质茚替达尼治疗诱导的小鼠肺纤维化的改善作用,该物质被认为是金标准。动物模型中的治疗将在最相关的临床结果中表现出与人类相似的效果,这一假设得到了检验。本研究采用了两种实验设计,一种预防方案,从激发当天开始治疗;以及治疗方案,从激发后第7天开始,此时肺部出现纤维化变化。实验在两个不同的时间点终止,即激发后14天和21天。C57BL/6小鼠从第0天至第14天或从第7天至第21天用博来霉素(BLM)鼻内给药并用茚替达尼处理。BLM激发后14或21天,使用体内侵入性肺功能测量系统Buxco®肺功能测试(PFT)(DSI)评估PFT™, 美国新布莱顿)。组织学评估作为改良的Ashcroft评分进行。博来霉素激发在两个实验中都诱导了FVC的显著降低。然而,两种方案中给予的宁替达尼治疗显著改善了肺功能。这些发现通过Matsuse修改的Ashcroft评分系统的组织学分析得到了证实。总之,在两个实验中,功能测试参数与宁替达尼的临床效果之间显示出良好的相关性:预防方案在激发后14天取样,治疗方案在激发前21天取样。基于这些发现,PFTs的实施可能是一个很好的平台,可以提高该模型的转化价值和潜在的新治疗方法。
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Veterinarska stanica
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