Veterinary parasitology, regional studies and reports最新文献

{"title":"Trypanosoma cruzi infection in American black bears (Ursus americanus): A case report in a cub from California and serologic survey for exposure in wild black bears from several states","authors":"Reece Hughes ,&nbsp;Raquel Francisco ,&nbsp;Kayla Garrett ,&nbsp;Kevin Willitts ,&nbsp;Brandon Munk ,&nbsp;Justin Brown ,&nbsp;Carlos Rodriguez ,&nbsp;Alexa Rosypal von Dohlen ,&nbsp;Sterling McCarrall ,&nbsp;T’Keyah Dennard ,&nbsp;Timothy Champion ,&nbsp;Tracy Brown-Fox ,&nbsp;Jennifer Strules ,&nbsp;Colleen Olfenbuttel ,&nbsp;Christopher DePerno ,&nbsp;Sarah A. Hamer ,&nbsp;Michael J. Yabsley","doi":"10.1016/j.vprsr.2024.101129","DOIUrl":"10.1016/j.vprsr.2024.101129","url":null,"abstract":"<div><div><em>Trypanosoma cruzi</em> is an important cause of disease and death in humans and dogs, and although wildlife infections are common, less is known about disease manifestations. A 12-week-old male American black bear (<em>Ursus americanus</em>) cub with mild lethargy and anorexia presented to a wildlife rehabilitation center in Lake Tahoe, California. The cub continued to become increasingly weak and showed decreasing interest in play and other activities. The cub was anemic and had increased γ-glutamyltransferase (GGT) liver enzymes. A large number of trypanosomes were noted on a thin blood smear. <em>Trypanosoma cruzi</em> was isolated in culture from a subsequent blood collection. Proliferative bony lesions were noted on radiographs, but this finding was considered unrelated to the <em>T. cruzi</em> infection. The number of parasites observed in thin blood smears dramatically dropped over time, but it remained PCR positive until at least nine months. The cub continued to gain weight and became increasingly active. Serum samples from the cub were positive with three different serologic assays (IFA, ELISA, and ICT). The bear was not treated because of the decreasing parasitemia and the improvement in activity and appetite. Although the bear could not be released due to issues unrelated to <em>T. cruzi</em>, it remains healthy in a captive facility. Sequence analysis of the DHFR-TS and COII-ND1 gene sequences confirmed the bear was infected with DTC TcIV. Following the detection of this clinical case, a serologic survey was conducted to determine the prevalence of <em>T. cruzi</em> exposure of black bears in California, North Carolina, and Pennsylvania. Because no serologic assay has been validated for use in bears, three different assays were used. Marked differences in apparent seroprevalence range from 1% (requiring all three assays to be positive) to ∼20.7% (requiring only one assay to be positive). Black bears are naturally exposed to <em>T. cruzi</em> across the United States. Future studies using PCR testing of tissues or blood would be needed to better understand the prevalence of <em>T. cruzi</em> in wild black bears, lineages most commonly associated with infection, and if <em>T. cruzi</em> represents a health threat to bears.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"56 ","pages":"Article 101129"},"PeriodicalIF":1.4,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142419731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gray fox (Urocyon cinereoargenteus) identified as a new intermediate host for Sarcocystis neurona","authors":"Jitender P. Dubey ,&nbsp;Aditya Gupta ,&nbsp;Rafael Calero-Bernal ,&nbsp;Larissa S. de Araujo ,&nbsp;María L. García-Gil ,&nbsp;Jaquin Battle ,&nbsp;Ankrah Ankarah ,&nbsp;Kyle Van Why ,&nbsp;Justin D. Brown ,&nbsp;Benjamin M. Rosenthal","doi":"10.1016/j.vprsr.2024.101122","DOIUrl":"10.1016/j.vprsr.2024.101122","url":null,"abstract":"<div><div>The protozoan <em>Sarcocystis neurona</em> can cause severe disease in horses, marine mammals, and several other animal species in North America. The Virginia opossum (<em>Didelphis virginiana</em>) is its definitive host, and the raccoon (<em>Procyon lotor</em>) has been considered its primary intermediate host in the USA. Although canids have not previously been identified as important intermediate hosts for this parasite, we here report several natural cases in the gray fox (<em>Urocyon cinereoargenteus</em>). We identified muscular infections in 11 (23.9 %) of 46 gray foxes from Pennsylvania, USA. In hematoxylin and eosin (HE)-stained sections of tongue and limb muscles, only 13 sarcocysts were detected in 7 of 46 foxes, in limb muscle of 4 and in tongue of 4. In HE-stained sections, the sarcocyst wall was up to 2.7 μm-thick and contained finger-like villar protrusions. In unstained muscle squashes, 44 sarcocysts were detected; they were up to 1200 μm long and 69 μm wide. From each infected fox, only one morphologic type of sarcocyst was found. By transmission electron microscopy, these sarcocysts appeared identical to those in animals experimentally infected with <em>S. neurona</em>. Molecularly, sarcocysts were characterized using <em>18S rRNA, 28S rRNA, cox1, ITS1, rpoB, RON1, RON2, RON3, GAPDH1, ROP20, ROP21, ROP39, SnSRS21</em> and <em>TUBA1</em>; results confirmed the presence of <em>S. neurona</em> in the gray fox. This is the first report of muscular sarcocysts in the gray fox.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"56 ","pages":"Article 101122"},"PeriodicalIF":1.4,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Amblyomma maculatum and Rickettsia parkeri in prairies of Central Missouri","authors":"Jaylon Vaughn ,&nbsp;Haylie J. Brown ,&nbsp;Anthony Ogunbadewa ,&nbsp;Solomon Odemuyiwa ,&nbsp;Deborah M. Anderson ,&nbsp;Samniqueka J. Halsey","doi":"10.1016/j.vprsr.2024.101126","DOIUrl":"10.1016/j.vprsr.2024.101126","url":null,"abstract":"<div><div>Prairie habitats are complex ecosystems experiencing destruction and decline due to landscape repurposing, thus resulting in significant changes to plant, animal, and insect wildlife diversity. In the Central United States, the reconstruction of prairie habitats from farmland is a widely applied strategy to raise diversity and recreate a healthy, complex ecosystem. In Central Missouri, we are examining the consequences of reconstruction efforts on the prevalence of zoonotic diseases and their associated pathogens and have observed large populations of Dog ticks, <em>Dermacentor variabilis</em>, and Lone Star ticks, <em>Amblyomma americanum</em>, as well as the first recorded instance of the Gulf Coast tick, <em>Amblyomma maculatum,</em> in Callaway County. The Gulf Coast tick is traditionally found mainly along the Atlantic and Gulf Coast regions, but has been undergoing geographic expansion in the last decade. Since detection in 2020, the number of Gulf Coast ticks has increased,. Upon screening 193 Gulf Coast ticks in 105 pools, we found that 6.67 % of ticks were positive for the pathogenic <em>Rickettsia parkeri,</em> which causes spotted fever rickettsiosis in animals and humans, with most ticks coming from the reconstructed prairie site. These observations suggest that reconstruction of prairie habitat has the potential to support the sylvatic cycle of <em>Rickettsia parkeri</em>. Collectively, our observations show that the reconstructed prairie are capable of harboring large tick populations as compared to remnant prairies and demonstrates a potential increase in disease risk as a result. This works highlights the importance of proactive surveillance of ticks, especially with land-use change.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"56 ","pages":"Article 101126"},"PeriodicalIF":1.4,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142419732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “First record of Hydatigera taeniaeformis in Geoffroy's cat (Leopardus geoffroyi) in Brazil” [Veterinary Parasitology: Regional Studies and Reports 54 (2024) 101100 1–5]","authors":"Julia Somavilla Lignon ,&nbsp;Tamires Silva dos Santos ,&nbsp;Giulia Ribeiro Meireles ,&nbsp;Kauê Rodriguez Martins ,&nbsp;Wesley Aquino Zoia ,&nbsp;Mauro Pereira Soares ,&nbsp;Fabiane de Holleben Camozzato Fadrique ,&nbsp;Rodrigo Casquero Cunha ,&nbsp;Silvia Gonzalez Monteiro ,&nbsp;Felipe Geraldo Pappen ,&nbsp;Diego Moscarelli Pinto ,&nbsp;Fábio Raphael Pascoti Bruhn","doi":"10.1016/j.vprsr.2024.101120","DOIUrl":"10.1016/j.vprsr.2024.101120","url":null,"abstract":"","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"56 ","pages":"Article 101120"},"PeriodicalIF":1.4,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142327071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of Fasciola hepatica obtained from cattle and horse in Central Chile","authors":"Gonzalo Cabrera ,&nbsp;Carolina Cabezas ,&nbsp;Daniela Estay-Olea ,&nbsp;Caroll Stoore ,&nbsp;María Soledad Baquedano ,&nbsp;Rodolfo Paredes ,&nbsp;Christian Hidalgo","doi":"10.1016/j.vprsr.2024.101130","DOIUrl":"10.1016/j.vprsr.2024.101130","url":null,"abstract":"<div><div>Liver fluke infection, caused by the trematode <em>Fasciola hepatica</em>, is a parasitic zoonotic disease affecting various mammals, including humans, and has significant implications for public, animal, and ecosystem health. This study provides the first genetic characterization of <em>F. hepatica</em> in Chile, focusing on the complete mitochondrial gene <em>cox1</em>. Samples were collected from two different host species: cattle and horses. Our findings revealed that 70 % of detected haplotypes were found in either cattle or horses, which coincides with their geographical origin. Interestingly, the use of full-length sequences resulted in the identification of 80 % unique sequences, whereas this reduced to 45 % when analyzing the traditionally used short sequences. This underestimation of genetic diversity suggests that broader sequencing efforts might be essential for a more accurate understanding of <em>F. hepatica</em> genetic landscape. This research underscores the importance of understanding the genetic variability in parasites to improve strategies for disease control and treatment.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"56 ","pages":"Article 101130"},"PeriodicalIF":1.4,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142419826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First morphological and molecular characterisation of Spirometra mansoni (Cestoda, Diphyllobothriidae) in a domestic cat from Veracruz, Mexico","authors":"Enrique Salazar-Grosskelwing ,&nbsp;Roger I. Rodriguez-Vivas ,&nbsp;Manuel E. Bolio-González ,&nbsp;Dora Romero-Salas ,&nbsp;Rodolfo Ramos-Beltrán ,&nbsp;Alberto Solano-Barquero ,&nbsp;Alicia Rojas","doi":"10.1016/j.vprsr.2024.101127","DOIUrl":"10.1016/j.vprsr.2024.101127","url":null,"abstract":"<div><div>Cestodes of the genus <em>Spirometra</em> are multi-host parasites that are the causative agents of spirometrosis in domestic and wild carnivores and sparganosis in humans, endemic diseases in tropical and subtropical regions. In domestic animals, the infection is usually asymptomatic or produces gastrointestinal signs such as vomiting and chronic diarrhea. In humans, an incidental parasitosis develops where the larva can lodge in tissues and cause a variety of symptoms, including neuropathies, blindness, paralysis, and death. Described here is a case of spirometrosis in a domestic cat treated in a veterinary clinic in Veracruz, Mexico. The cat presented with an approximate 50 cm section of the strobila protruding from the anus. The proglottids and eggs of the cestode were described via morphological analyses. DNA was obtained from the proglottids for the amplification and sequencing of a fragment of the cytochrome oxidase subunit 1 (<em>cox</em>1) gene. Genetic analysis demonstrated 100 % similarity to <em>Spirometra mansoni</em>. The cat was treated orally with ivermectin/praziquantel (2 mg/50 mg), and the dose was repeated 24 h later. The cat was observed daily for seven days, and no adverse effects occurred. On days 8 and 68 post-treatment, coprological examination revealed no evidence of the parasite. This is the first report of spirometrosis in cats from Mexico.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"56 ","pages":"Article 101127"},"PeriodicalIF":1.4,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of Toxocara vitulorum infection in a dairy calf in Tennessee","authors":"Heidi Wyrosdick,&nbsp;Alexis Johnson,&nbsp;Kathleen Riese,&nbsp;Debra Miller,&nbsp;Richard Gerhold","doi":"10.1016/j.vprsr.2024.101128","DOIUrl":"10.1016/j.vprsr.2024.101128","url":null,"abstract":"<div><div>The ascarid nematode, <em>Toxocara vitulorum</em> is a destructive parasite of ruminants with a world-wide distribution that causes mortality in calves, especially those with poor nutrition. Reports of <em>Toxocara vitulorum</em> are scarce in the continental United States and Canada. A single gravid female <em>T. vitulorum</em> was discovered on the necropsy of a 2.5-month-old mini jersey calf from a dairy farm in Sparta, TN. Genetic analysis of the 18S and COI genes revealed a 100 % match to <em>T. vitulorum</em> samples isolated from beef calves in Iowa and a 96.73 % match to <em>T. vitulorum</em> isolated from a yak in Austria, respectively. Although perforation of the small intestine was seen in the calf and is the typical cause of death in calves with high burdens of this worm, it is unknown if this single nematode infection contributed to the cause of death in this case. The source of the infection in this calf also remains unknown. Fecal centrifugal flotations on the rest of the herd (<em>n</em> = 8) did not produce any <em>T. vitulorum</em> eggs, even in the 2 calves that were under 6 months old on the farm. We were unable to obtain milk to test for <em>T. vitulorum</em> larvae from the calf's mother. This is the first report of <em>T. vitulorum</em> in dairy calves in the US and the first report of this parasite in Tennessee. Given the devastation caused by this parasite in cattle and bison herds in other countries, further studies to determine the prevalence and pathogenicity of this parasite in the US are vital to prevent spread of this parasite to other vulnerable species and to prevent economic losses in the animal industry.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"56 ","pages":"Article 101128"},"PeriodicalIF":1.4,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First record of feline urinary bladder worm (Capillaria syn. Pearsonema) in Sri Lanka: Clinical, histopathological and molecular characterization","authors":"Tilini K. De Silva ,&nbsp;Shobhath Wijeratne ,&nbsp;Piyumali K. Perera ,&nbsp;Kavindra Wijesundera ,&nbsp;Waruni R. Jayaweera ,&nbsp;Vindya Perera ,&nbsp;Rupika S. Rajakaruna","doi":"10.1016/j.vprsr.2024.101117","DOIUrl":"10.1016/j.vprsr.2024.101117","url":null,"abstract":"<div><div>Nematode infections in the urinary tract of domestic cats and dogs are rare, although common in wild predatory animals. Here we report a case of a urinary bladder capillariosis in a cat from Sri Lanka. A three-year-old domestic male cat from the Kalutara District was brought to a private veterinary hospital due to frequent urination, vomiting, and loss of appetite. The physical examinations reported severe emaciation, gasping and abdominal breathing and retarded growth. Successive to urinary sedimentation, <em>Capillaria</em>-type eggs were detected. Blood Urea Nitrogen level of 28.02 Mmol/L and serum creatinine level of 0.42 mmol/L were higher than the normal ranges. Necropsy revealed an enlarged left kidney with oedematous renal parenchyma. Following the histopathological examination, multifocal areas of tubular degeneration and necrosis with multifocal fibroblast proliferation were observed in the renal cortex. The lungs showed diffuse pulmonary oedema and chronic interstitial pneumonia. Molecular analysis with PCR and DNA sequencing of the <em>Capillaria</em>-type eggs in sedimented urine, using an amplicon (<em>18S rRNA</em>) for <em>Capillaria</em> (563 bp) followed by phylogenetic analysis revealed a 100 % identity to <em>Pearsonema</em> collected from the urinary bladder of a raccoon from Japan. Cases of <em>Capillaria</em> in cats are rarely reported because most infected animals do not show clinical signs. This is the first case report of <em>Capillaria</em> syn. <em>Pearsonema</em> sp. identifies and characterizes the species using molecular tools and describes the infection's clinical symptoms.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"56 ","pages":"Article 101117"},"PeriodicalIF":1.4,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection of Plasmodium huffi (Apicomplexa: Haemosporida) in birds from Mato Grosso state, midwestern Brazil with new host data","authors":"Morgana Maira Hennig ,&nbsp;Luiz Gustavo Magalhães Alves ,&nbsp;Victória Luiza de Barros Silva ,&nbsp;Rosa Helena dos Santos Ferraz ,&nbsp;Thais Oliveira Morgado ,&nbsp;Érika Martins Braga ,&nbsp;Richard de Campos Pacheco","doi":"10.1016/j.vprsr.2024.101118","DOIUrl":"10.1016/j.vprsr.2024.101118","url":null,"abstract":"<div><div>Haemosporidians are heteroxenous, cosmopolitan protozoa that infect birds, using hematophagous dipterans as vectors. The genus <em>Plasmodium</em> has a moderate species diversity and is known for great flexibility in alternating host species, mild to severe pathogenicity, and generally low prevalence. <em>Plasmodium huffi</em> was first described in 1950 and considered insufficiently characterized, however, in 2021, this species was re-described as infecting two different Brazilian toucans, <em>Ramphastos toco</em> and <em>Pteroglossus aracari.</em> Herein, we describe four new potential avian host species for <em>P. huffi</em>: <em>Pavo cristatus, Cariama cristata, Rhea americana,</em> and <em>Porphyrio martinica</em> by employing molecular diagnostics and conducting phylogenetic analysis, providing the detection of the RTCE206 and PACE173 lineages of <em>P. huffi</em>, previously described in Toco Toucan and Black-necked Araçari, respectively. Our study reinforces the data in the literature on the great flexibility of the genus <em>Plasmodium</em> in alternating host species. In addition, it highlights the current knowledge about <em>P. huffi</em> encompassing its underestimated potential for host diversity. Further research is essential, particularly involving bird groups frequently excluded from haemosporidian studies in Brazil, such as non-passeriform birds, to accurately elucidate the <em>P</em>. <em>huffi</em> host-parasite relationships.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"56 ","pages":"Article 101118"},"PeriodicalIF":1.4,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142318764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of mutations in the voltage-gated sodium channel gene of Varroa destructor (Acari: Varroidae) populations","authors":"İsmail Karakaya,&nbsp;Batuhan Aşkım Arslanhan,&nbsp;Zuhal Önder","doi":"10.1016/j.vprsr.2024.101121","DOIUrl":"10.1016/j.vprsr.2024.101121","url":null,"abstract":"<div><div>The honeybee (<em>Apis mellifera</em>) ectoparasite, <em>Varroa destructor,</em> is one of the most important honeybee pests worldwide. Acaricides, including the pyrethroids (tau-fluvalinate, flumethrin), and organophosphate (coumaphos) have been applied to control this mite within apiaries, still the long-term, constant, and excessive use of these products has led to the development of resistance in many populations. Three different mutations (L925V, L925I, L925M) at position 925 and one mutation (M918L) at 918 position of the <em>V</em>. <em>destructor</em> voltage-gated sodium channel (VGSC) have been associated with the resistance to these compounds. In the present study, we examined the presence of resistance mutations in the VGSC gene, encoding the target of pyrethroids, in the <em>V. destructor</em> population collected from the Kayseri and Sivas provinces of Central Anatolia Region of Türkiye. A total of 200 <em>V. destructor</em> samples were collected from 20 apiaries in two provinces throughout 2023. To investigate the mutations in <em>Varroa</em> samples, the domain II region of the VGSC gene was amplified using PCR and sequenced. The nucleotide sequencing of the IIS4-IIS5 linker region of the VGSC gene revealed one amino acid change at position 925: a leucine to isoleucine substitution (L925I). No mutations at other positions were identified. Homozygous resistant alleles were detected in 20 (40 %) of the sequenced 50 samples in the study areas. However, we detected the homozygous sensitive allele (wild-type allele, L925) in the remaining samples (50/30, 60 %). The result shows that this status may indicate a problem for <em>Varroa</em> control in the future. Thus, alternative acaricide with a mode of action of different pyrethroids should be considered in the control of <em>V. destructor</em> populations in these provinces.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"56 ","pages":"Article 101121"},"PeriodicalIF":1.4,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}